188 results match your criteria cd4+t


Immune profiles and DNA methylation alterations related with non-muscle-invasive bladder cancer outcomes.

Clin Epigenetics 2022 Jan 21;14(1):14. Epub 2022 Jan 21.

Department of Epidemiology, Geisel School of Medicine, Dartmouth College, Lebanon, NH, 03766, USA.

Background: Non-muscle-invasive bladder cancer (NMIBC) patients receive frequent monitoring because ≥ 70% will have recurrent disease. However, screening is invasive, expensive, and associated with significant morbidity making bladder cancer the most expensive cancer to treat per capita. There is an urgent need to expand the understanding of markers related to recurrence and survival outcomes of NMIBC. Read More

View Article and Full-Text PDF
January 2022

[Pathogenesis of ANCA-associated vasculitides in 2021: An update].

Rev Med Interne 2022 Jan 12. Epub 2022 Jan 12.

Service de médecine interne, CHU de Nantes, Nantes, France; Inserm, centre de recherche en transplantation et immunologie, UMR 1064, université de Nantes, Nantes, France.

Anticytoplasmic neutrophil antibodies (ANCA)-associated vasculitis (AAV) are rare systemic immune-mediated diseases characterized by small vessel necrotizing vasculitis and/or respiratory tract inflammation. Over the last 2 decades, anti-MPO vasculitis mouse model has enlightened the role of ANCA, neutrophils, complement activation, T helper cells (Th1, Th17) and microbial agents. In humans, CD4T cells have been extensively studied, while the dramatic efficacy of rituximab demonstrated the key role of B cells. Read More

View Article and Full-Text PDF
January 2022

The Cultured T Model of HIV Latency.

Authors:
Alberto Bosque

Methods Mol Biol 2022 ;2407:45-55

Department of Microbiology, Immunology and Tropical Medicine, George Washington University, Washington, DC, USA.

Models to study HIV latency have improved our understanding of the mechanisms involved in this process and have helped in the discovery and development of therapeutic strategies to eradicate HIV. Primary cell models are based on the in vitro generation of latently infected cells using CD4T cells isolated from blood, lymph nodes or other lymphoid organs. In this chapter, we describe the generation of HIV latently infected memory CD4T cells using blood naïve CD4T cells from peripheral blood with a phenotype resembling that of central memory CD4T cells. Read More

View Article and Full-Text PDF
January 2022

Apolipoprotein D as a Potential Biomarker and Construction of a Transcriptional Regulatory-Immune Network Associated with Osteoarthritis by Weighted Gene Coexpression Network Analysis.

Cartilage 2021 12 31;13(1_suppl):1702S-1717S. Epub 2021 Oct 31.

Department of Orthopedics Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

Objective: Synovial inflammation influences the progression of osteoarthritis (OA). Herein, we aimed to identify potential biomarkers and analyze transcriptional regulatory-immune mechanism of synovitis in OA using weighted gene coexpression network analysis (WGCNA).

Design: A data set of OA synovium samples (GSE55235) was analyzed based on WGCNA. Read More

View Article and Full-Text PDF
December 2021

The association between serum CD4 T lymphocyte counts and surgical outcomes in HIV/AIDS patients in Guangxi, China: a retrospective cohort study.

PeerJ 2021 20;9:e12023. Epub 2021 Sep 20.

Longtan Hospital of Guangxi Zhuang Autonomous Region, Liuzhou, Guangxi, China.

Background: HIV/AIDS is a chronic disease leading to complications in infected individuals that often require surgical intervention. These patients' serum CD4 T lymphocyte (CD4) counts represent one of the most important indicators of their ability to tolerate surgical treatment. Previous studies have demonstrated that CD4 cell count (CD4-CC) < 200 cells/μl may increase the risk of surgical complications in these patients, limiting their ability to undergo surgery, which may negatively affect their quality of life. Read More

View Article and Full-Text PDF
September 2021

In vivo kinetics of peripheral cellular immune responses in Mycobacterium avium subspecies paratuberculosis (MAP) infected and vaccinated goats.

Comp Immunol Microbiol Infect Dis 2021 Dec 20;79:101710. Epub 2021 Sep 20.

Division of Animal Health, Central institute for Research on Goats (CIRG), Makhdoom, Farah, Mathura, UP 281122, India.

Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of paratuberculosis (ParaTB) also known as Johne's disease (JD) in ruminants, which is characterized by chronic intestinal inflammation. A similar counterpart has been observed in the form of Crohn's disease in humans. The present study is the first trail in goats to understand the peripheral cellular immune responses following experimental MAP infection and vaccination. Read More

View Article and Full-Text PDF
December 2021

Characterisation of peri-implantation endometrial Treg and identification of an altered phenotype in recurrent pregnancy loss.

Mucosal Immunol 2022 Jan 22;15(1):120-129. Epub 2021 Sep 22.

Nuffield Department of Women's and Reproductive Health, L3 Women's Centre, John Radcliffe Hospital, University of Oxford, Oxford, UK.

Recurrent Pregnancy Loss (RPL) affects 2-4% of couples, and with increasing numbers of pregnancy losses the risk of miscarrying a euploid pregnancy is increased, suggesting RPL is a pathology distinct from sporadic miscarriage that is due largely to lethal embryonic aneuploidy. There are a number of conditions associated with RPL including unspecified "immune" pathologies; one of the strongest candidates for dysregulation remains T regulatory cells as depletion in the very early stages of pregnancy in mice leads to pregnancy loss. Human endometrial Treg and conventional CD4T cells were isolated during the peri-implantation period of the menstrual cycle in normal women. Read More

View Article and Full-Text PDF
January 2022

Association between frailty phenotype, quantification of plasma HIV-1 RNA, CD4 cell count and HAART in HIV-positive subjects: a systematic review and meta-analysis of observational studies.

AIDS Care 2021 Jul 22:1-10. Epub 2021 Jul 22.

Investigative Pathology Laboratory, Federal University of Sergipe, Aracaju, Brazil.

HIV infection causes a constant activation of the immune system and contributes to an enhanced systemic pro-inflammatory cytokine milieu, which has been associated with premature aging and frailty. We performed a systematic review and meta-analysis to analyze whether the HIV-1 RNA load, CD4+ T-lymphocyte counts and exposure to HAART in HIV-positive subjects are associated with frailty phenotype. Searches were performed in PubMed, SCOPUS, Lilacs, Web of Science, Google Scholar, and OpenThesis databases. Read More

View Article and Full-Text PDF

Immune characteristics of human immunodeficiency virus/severe acute respiratory syndrome coronavirus 2 coinfection: A case report and mini-review.

SAGE Open Med Case Rep 2021 16;9:2050313X211014958. Epub 2021 Jun 16.

Department of Infectious Diseases, Longtan Hospital of Guangxi Zhuang Autonomous Region, Liuzhou, China.

Since its first appearance in Wuhan, China, severe acute respiratory syndrome coronavirus 2 has rapidly spread throughout the world and has become a global pandemic. It remains unclear whether people living with human immunodeficiency virus are at an increased risk of coronavirus disease 2019 and severe disease manifestation; until now, the evidence regarding the outcomes from severe acute respiratory syndrome coronavirus 2 infection in people living with human immunodeficiency virus is still limited and conflicting. The clinical characteristics of seven patients of family cluster-onset coronavirus disease 2019 were reported, including the immune characteristics of one patient of human immunodeficiency virus/severe acute respiratory syndrome coronavirus 2 coinfection. Read More

View Article and Full-Text PDF

Efficacy and Safety of Triple versus Dolutegravir-based Dual Therapy in Patients with HIV-1 Infection: A Meta-analysis of Randomized Controlled Trials.

AIDS Rev 2021 06 3;23(3):133-142. Epub 2021 Jun 3.

Department of Evidence-based Medicine and Clinical Center; Department of Clinical Pharmacy; The First People's Hospital of Huaihua, University of South China, Huaihua, China.

A new strategy of simplification therapy shown the unique benefits in clinical treatment, by reducing pill burden and avoid drug exposure. To provide more evidence for the strategy, we compared the efficacy and safety of dolutegravir (DTG)-containing simplified dual combination antiretroviral therapy (cART) and traditional triple cART for people living with HIV/AIDS. The meta-analysis of randomized controlled trials compared DTG-containing dual therapy with triple cART. Read More

View Article and Full-Text PDF

Alternative Splicing Events in Tumor Immune Infiltration in Colorectal Cancer.

Front Oncol 2021 29;11:583547. Epub 2021 Apr 29.

Clinical Evaluation Center, Chinese Academy of Chinese Medical Sciences, Beijing, China.

Despite extensive research, the exact mechanisms involved in colorectal cancer (CRC) etiology and pathogenesis remain unclear. This study aimed to examine the correlation between tumor-associated alternative splicing (AS) events and tumor immune infiltration (TII) in CRC. We analyzed transcriptome profiling and clinical CRC data from The Cancer Genome Atlas (TCGA) database and lists of AS-related and immune-related signatures from the SpliceSeq and Innate databases, respectively to develop and validate a risk model of differential AS events and subsequently a TII risk model. Read More

View Article and Full-Text PDF

Diagnostic value of serum human Galactomannan aspergillus antigen and 1,3-beta-D-glucan in immunocompromised patient suspected fungal infection.

J Clin Lab Anal 2021 Jun 4;35(6):e23806. Epub 2021 May 4.

Pulmonology and Respiratory Department of Faculty Medicine Brawijaya University, Dr. Saiful Anwar General Hospital, Malang, Indonesia.

Background: The prevalence of fungal infection (FI) in developing countries is high, but the diagnosis of FI is still challenging to determine, so it is needed evaluation of biomarkers other than microbiological culture, because the culture has low sensitivity, high cost, not available in every laboratory and needs a long time. The detection of human galactomannan Aspergillus antigen (GAL) and 1,3-beta-D-glucan (BDG) on the fungal cell wall could be the promising biomarkers for fungal infection. Neutropenia, lymphopenia and CD4T cells in the immunocompromised patients are essential factors, but these cell associations with BDG and GAL levels have not been evaluated yet. Read More

View Article and Full-Text PDF

IL-21-Deficient T Follicular Helper Cells Support B Cell Responses Through IL-27 in Patients With Chronic Hepatitis B.

Front Immunol 2020 28;11:599648. Epub 2021 Jan 28.

Division of Clinical Care and Research, Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD, United States.

Chronic Hepatitis B (CHB) affects over 350 million people worldwide. Current treatment does result in reduced complications; however, a cure (development of antibodies to the S antigen) is not achieved, requiring life-long therapy. Humoral responses contribute to viral elimination by secreting neutralizing antibodies; though, effective induction of humoral immunity require CD4T cell differentiation into T follicular helper (T) cells that support B cell response through interleukin-21 (IL-21). Read More

View Article and Full-Text PDF

A Longitudinal Epigenetic Aging and Leukocyte Analysis of Simulated Space Travel: The Mars-500 Mission.

Cell Rep 2020 12 25;33(10):108406. Epub 2020 Nov 25.

Division of Environmental Health Sciences, School of Public Health and Center for Computational Biology, University of California, Berkeley, Berkeley, CA 94720, USA.

Astronauts undertaking long-duration space missions may be vulnerable to unique stressors that can impact human aging. Nevertheless, few studies have examined the relationship of mission duration with DNA-methylation-based biomarkers of aging in astronauts. Using data from the six participants of the Mars-500 mission, a high-fidelity 520-day ground simulation experiment, we tested relationships of mission duration with five longitudinally measured blood DNA-methylation-based metrics: DNAmGrimAge, DNAmPhenoAge, DNA-methylation-based estimator of telomere length (DNAmTL), mitotic divisions (epigenetic mitotic clock [epiTOC2]), and pace of aging (PoA). Read More

View Article and Full-Text PDF
December 2020

Rescuing ESAT-6 Specific CD4 T Cells From Terminal Differentiation Is Critical for Long-Term Control of Murine Mtb Infection.

Front Immunol 2020 6;11:585359. Epub 2020 Nov 6.

Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark.

In most cases, (Mtb) causes life-long chronic infections, which poses unique challenges for the immune system. Most of the current tuberculosis (TB) subunit vaccines incorporate immunodominant antigens and at this point, it is poorly understood how the CD4 T cell subsets recognizing these antigens are affected during long-term infection. Very little is known about the requirements for sustainable vaccine protection against TB. Read More

View Article and Full-Text PDF

Alterations of Specific Lymphocytic Subsets with Aging and Age-Related Metabolic and Cardiovascular Diseases.

Life (Basel) 2020 Oct 17;10(10). Epub 2020 Oct 17.

School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan.

To investigate the association of immunosenescence with aged-related morbidity in the elderly, a clinical study was conducted to analyze and compare the alterations in peripheral blood (PB) T-cell subsets among young healthy (YH) controls, elderly healthy (EH) controls, and age-matched elderly patients with metabolic diseases (E-MDs), with cardiovascular diseases (E-CVDs) or with both (E-MDs/E-CVDs). The frequencies of CD3T, CD8T and invariant natural killer T (iNKT) cells were decreased in the EH, E-MD and E-CVD cohorts, indicating a decline in defense function. Although CD4T and regulatory T (Treg) cell frequencies tended to increase with aging, they were lower in patients with E-MDs and E-CVDs. Read More

View Article and Full-Text PDF
October 2020

The metabolic enzyme arginase-2 is a potential target for novel immune modulatory vaccines.

Oncoimmunology 2020 06 1;9(1):1771142. Epub 2020 Jun 1.

National Center for Cancer Immune Therapy (CCIT-DK), Department of Oncology, Copenhagen University Hospital Herlev, Copenhagen, Denmark.

One way that tumors evade immune destruction is through tumor and stromal cell expression of arginine-degrading enzyme arginase-2 (ARG2). Here we describe the existence of pro-inflammatory effector T-cells that recognize ARG2 and can directly target tumor and tumor-infiltrating cells. Using a library of 34 peptides covering the entire ARG2 sequence, we examined reactivity toward these peptides in peripheral blood mononuclear cells from cancer patients and healthy individuals. Read More

View Article and Full-Text PDF

Role of the adaptive immune system in atherosclerosis.

Authors:
Klaus Ley

Biochem Soc Trans 2020 10;48(5):2273-2281

Center for Autoimmunity and Inflammation, Inflammation Biology Laboratory, La Jolla Institute for Immunology, 9420 Athena Circle Drive, La Jolla, CA 92037, U.S.A.

Atherosclerosis, the pathology underlying heart attacks, strokes and peripheral artery disease, is a chronic inflammatory disease of the artery wall initiated by elevated low-density lipoprotein (LDL) cholesterol levels. LDL accumulates in the artery wall, where it can become oxidized to oxLDL. T cell responses to ApoB, a core protein found in LDL and other lipoproteins, are detectable in healthy mice and people. Read More

View Article and Full-Text PDF
October 2020

Systematic Immunophenotyping Reveals Sex-Specific Responses After Painful Injury in Mice.

Front Immunol 2020 29;11:1652. Epub 2020 Jul 29.

Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, United States.

Many diseases display unequal prevalence between sexes. The sex-specific immune response to both injury and persistent pain remains underexplored and would inform treatment paradigms. We utilized high-dimensional mass cytometry to perform a comprehensive analysis of phenotypic and functional immune system differences between male and female mice after orthopedic injury. Read More

View Article and Full-Text PDF

Genome-wide DNA methylation differences and polychlorinated biphenyl (PCB) exposure in a US population.

Epigenetics 2021 03 22;16(3):338-352. Epub 2020 Jul 22.

Departments of Gynecology and Obstetrics & Psychiatry and Behavioral Science, Emory University School of Medicine , Atlanta, GA, USA.

Exposure to polychlorinated biphenyls (PCBs), an endocrine-disrupting compound, is ubiquitous despite decades-old bans on the manufacture and use of PCBs. Increased exposure to PCBs is associated with adverse health consequences throughout life, including type 2 diabetes and cancer. PCB exposure is also associated with alterations in epigenetic marks and gene transcription, which could lead to adverse health outcomes, but many of these are population-specific. Read More

View Article and Full-Text PDF

Effects of Adenovirus-Mediated Overexpression of JAZF1 on Chronic Inflammation: An In Vitro and In Vivo Study.

Med Sci Monit Basic Res 2020 Jul 13;26:e924124. Epub 2020 Jul 13.

Gene Diagnostics Center of Medical Laboratory, Chongqing Three Gorges Central Hospital, Chongqing, China (mainland).

BACKGROUND Insulin sensitivity and inflammation can be affected by juxtaposition with another zinc finger gene 1 (JAZF1), but its precise role in chronic inflammation is unclear. In this study, JAZF1-overexpression adenovirus plasmids were transfected into macrophages, CD4⁺ T cells, and C57BL/6J mice to assess the role of JAZF1 in chronic inflammation. MATERIAL AND METHODS JAZF1 was cloned into an adenovirus skeleton plasmid and transfected in HEK293 cells to package and enrich the virus particles. Read More

View Article and Full-Text PDF

MiR-1165-3p Suppresses Th2 Differentiation via Targeting IL-13 and PPM1A in a Mouse Model of Allergic Airway Inflammation.

Allergy Asthma Immunol Res 2020 Sep;12(5):859-876

NHC Key Laboratory of Antibody Technique, Nanjing Medical University, Nanjing, Jiangsu, China.

Purpose: CD4⁺T cells are essential in the pathogenesis of allergic asthma. We have previously demonstrated that microRNA-1165-3p (miR-1165-3p) was significantly reduced in T-helper type (Th) 2 cells and that miR-1165-3p was a surrogate marker for atopic asthma. Little is known about the mechanisms of miR-1165-3p in the regulation of Th2-dominated allergic inflammation. Read More

View Article and Full-Text PDF
September 2020

TLR7 Polymorphism (rs179008 and rs179009) in HIV-Infected Individual Naïve to ART.

Mediators Inflamm 2020 20;2020:6702169. Epub 2020 May 20.

Division of ECD, Indian Council of Medical Research-HQ, New Delhi 110029, India.

Toll-like receptors (TLRs) play an important role in the innate immune response to HIV infection. Single nucleotide polymorphism (SNP) in (Gln11Leu) gene has been associated with a rapid decline of CD4T cell count. Hence, we assessed the (rs179008, Gln11Leu (A/T) and rs179009, IVS2-151 (A/G)) polymorphism in 150 HIV-infected individuals naïve to ART and 158 healthy controls. Read More

View Article and Full-Text PDF

Critical Role of TSLP Receptor on CD4 T Cells for Exacerbation of Skin Inflammation.

J Immunol 2020 07 22;205(1):27-35. Epub 2020 May 22.

Department of Immunology and Pathology, Research Institute, National Center for Global Health and Medicine, Ichikawa-shi, Chiba 272-8516, Japan;

Thymic stromal lymphopoietin (TSLP) is a key cytokine that initiates and promotes allergic inflammation both in humans and mice. It is well known that TSLP is important in initial step of inflammation by stimulating dendritic cells to promote Th2 differentiation of naive T cells. However, TSLP is abundantly produced in the late phase of inflammation, as well; therefore, we focused on the function of TSLP in chronic Th2-type inflammation. Read More

View Article and Full-Text PDF

Correction to: Cellular immunotherapy using irradiated lung cancer cell vaccine co-expressing GM-CSF and IL-18 can induce significant antitumor effects.

BMC Cancer 2020 01 17;20(1):42. Epub 2020 Jan 17.

State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, The People's Republic of China.

Following publication of the original article [1], the authors reported an error in Fig 5 of this article, graphs presenting FCM and immunofluorescent for CD4T, CD8T and NK cell of the Control Groups (LL2, LL2-irradation, MCS-irradiation) were inadvertently duplicated from another parallel experiment. Read More

View Article and Full-Text PDF
January 2020

Immune profile at HIV infection diagnosis: Evolution in the French Alps area over the last 20 years.

Med Mal Infect 2020 Aug 19;50(5):428-432. Epub 2019 Nov 19.

Comité régional de coordination de la lutte contre le VIH de l'Arc Alpin, Grenoble, France; Fédération d'infectiologie multidisciplinaire de l'Arc Alpin, université Grenoble-Alpes, Grenoble, France; Service des maladies infectieuses, CHU Grenoble Alpes, Grenoble, France.

Objectives: Health of HIV-infected people relies on early antiretroviral therapy, i.e. early diagnosis. Read More

View Article and Full-Text PDF

High rates of antiretroviral coverage and virological suppression in HIV-1-infected children and adolescents.

Med Mal Infect 2020 May 10;50(3):269-273. Epub 2019 Nov 10.

Unité d'immunologie, hématologie et rhumatologie pédiatrique, hôpital Necker-Enfants malades, AP-HP, 149, rue de Sèvres, 75015 Paris, France; Laboratoire de microbiologie clinique, hôpital Necker-Enfants malades, AP-HP, 149, rue de Sèvres, 75015 Paris, France; EHU 7328, institut Imagine, Sorbonne Paris Cité, université Paris Descartes, 149, rue de Sèvres, 75015 Paris, France. Electronic address:

Objectives: To assess the outcome of HIV-infected individuals attending one of the largest French pediatric HIV centers in 2016-2017 and to compare the rates of antiretroviral coverage and virological suppression with the UNAIDS targets.

Patients And Methods: The clinical and immuno-virological status of 163 HIV-1-infected children and adolescents attending Necker Hospital in Paris, France, were investigated. Virological suppression was defined as an HIV-1 viral load<50 copies/mL for at least six months. Read More

View Article and Full-Text PDF

Longitudinal Intra- and Inter-individual variation in T-cell subsets of HIV-infected and uninfected men participating in the LA Multi-Center AIDS Cohort Study.

Medicine (Baltimore) 2019 Oct;98(41):e17525

Department of Epidemiology, UCLA Fielding School of Public Health.

To assess the intra-individual and inter-individuals biological variation and the effect of aging on lymphocyte T-cells subsets.We assessed lymphocyte phenotypes (CD3, CD4, and CD8 T-cells) in 89 HIV-1-infected and 88 uninfected white non-Hispanic men every 6 months, to examine the biological variation for those measurements, and the average change in lymphocyte phenotype over 34 years.The markers showed significant intra-individuality in HIV-infected and uninfected individuals with index of individuality of <1. Read More

View Article and Full-Text PDF
October 2019

PD-1 Expression and Function of T-Cell Subsets in TILs From Human Lung Cancer.

J Immunother 2019 10;42(8):297-308

Key Laboratory of Tropical Disease Control of Sun Yat-Sen University, Ministry of Education, The Institute of Immunology of Zhong Shan Medical School, Sun Yat-Sen University, Guangzhou, Guangdong Province, China.

On the basis of the autologous tumor-infiltrating lymphocytes (TILs) or genetically modified TILs for adoptive cell therapy have received more attention. Programmed cell death protein 1 (PD-1) expression on the T cells exert complex response during the tumor immune response. But the composition and function of PD-1T-cell subsets in TILs from human lung cancer still limited. Read More

View Article and Full-Text PDF
October 2019

Strength training improves body composition, muscle strength and increases CD4+ T lymphocyte levels in people living with HIV/AIDS.

Infect Dis Rep 2019 Feb 23;11(1):7925. Epub 2019 May 23.

Department of Biomedical Sciences, University of Rio Grande do Norte State, Mossoró.

The establishment of physical training programs for people living with HIV/AIDS (PLWHA) has several benefits. The study aimed to analyze the effect of resistance training using prediction of intensity by subjective perception of effort (SPE) on body composition, muscle strength, and TCD4 lymphocyte levels in PLWHA. This a randomized controlled trial study. Read More

View Article and Full-Text PDF
February 2019