616 results match your criteria cd103- cd4+


The molecular basis and cellular effects of distinct CD103 expression on CD4 and CD8 T cells.

Cell Mol Life Sci 2021 Jun 15. Epub 2021 Jun 15.

Experimental Immunology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD, 20892, USA.

Integrin CD103 mediates the adhesion and tissue retention of T cells by binding to E-cadherin which is abundant on epithelial cells. Notably, CD103 is highly expressed on CD8 T cells but conspicuously absent on most CD4 T cells. The mechanism controlling such lineage-specific expression of CD103 remains unclear. Read More

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Type 17 immunity promotes the exhaustion of CD8 T cells in cancer.

J Immunother Cancer 2021 Jun;9(6)

Lab of Immune Regulation, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, South Korea

Background: Multiple types of immune cells producing IL-17 are found in the tumor microenvironment. However, their roles in tumor progression and exhaustion of CD8 tumor-infiltrating lymphocytes (TILs) remain unclear.

Methods: To determine the role of type 17 immunity in tumor, we investigated the growth of B16F10 melanoma and the exhaustion of CD8 TILs in mice, mice, RORγt inhibitor-treated mice, or their respective control mice. Read More

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Signatures and Specificity of Tissue-resident Lymphocytes Identified in Human Renal Peri-tumor and Tumor Tissue.

J Am Soc Nephrol 2021 Jun 1. Epub 2021 Jun 1.

K Kotsch, Department for General-, Visceral- and Vascular Surgery, Charite Medical Faculty Berlin, Berlin, Germany

Background: Tissue-resident memory (T) T cells are known to be important for the first line of defense in mucosa-associated tissues. However, the composition, localization, effector function, and specificity of T T cells in the human kidney and their relevance for renal pathology have not been investigated.

Methods: Lymphocytes derived from blood, renal peri-tumor samples, and tumor samples were phenotypically and functionally assessed by applying flow cytometry and highly advanced histology (Multi-Epitope Ligand Cartography) methods. Read More

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Involvement of Dendritic Cells and Th17 Cells in Induced Tertiary Lymphoid Structures in a Chronic Beryllium Disease Mouse Model.

Mediators Inflamm 2021 6;2021:8845966. Epub 2021 May 6.

Department of Pulmonary Medicine, Erasmus University Medical Center (Erasmus MC), Dr. Molewaterplein 50, 3015 GE Rotterdam, Netherlands.

Objective: To study airway pathophysiology and the role of dendritic cells (DCs) and IL-17 receptor (IL-17R) signals in a mouse model for CBD.

Methods: Here, we present a CBD mouse model in which mice were exposed to beryllium during three weeks. We also exposed IL-17R-deficient mice and mice in which DCs were depleted. Read More

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Expression pattern of tissue-resident memory T cells in cutaneous lupus erythematosus.

Lupus 2021 May 20:9612033211017218. Epub 2021 May 20.

Department of Dermatology, Gachon University Gil Medical Center, Gachon University College of Medicine, Incheon, Korea.

Background: Tissue resident memory T cells (TRMs) persist long-term in peripheral tissues without recirculation, triggering an immediate protective inflammatory state upon the re-recognition of the antigen. Despite evidence incriminating the dysregulation of TRMs in autoimmune diseases, few studies have examined their expression in cutaneous lupus erythematosus (CLE).

Objectives: We aimed to examine whether there are differences among TRM populations in CLE depending on different clinical conditions, such as the CLE subtype or association with systemic lupus erythematosus, and to determine the effect of type I interferon (IFN) on the development of TRMs in CLE. Read More

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CD31, CD38, CD44, and CD103 lymphocytes in peripheral blood, bronchoalveolar lavage fluid and lung biopsy tissue in sarcoid patients and controls.

J Thorac Dis 2021 Apr;13(4):2300-2318

Clinic of Chest Diseases, Immunology and Allergology of Faculty of Medicine, Vilnius University, Vilnius, Lithuania.

Background: The mechanisms driving the transition from inflammation to fibrosis in sarcoidosis patients are poorly understood; prognostic features are lacking. Immune cell profiling may provide insights into pathogenesis and prognostic factors of the disease. This study aimed to establish associations in simultaneous of lymphocyte subset profiles in the blood, bronchoalveolar lavage fluid (BALF), and lung biopsy tissue in the patients with newly diagnosed sarcoidosis. Read More

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The parasite cytokine mimic Hp-TGM potently replicates the regulatory effects of TGF-β on murine CD4 T cells.

Immunol Cell Biol 2021 May 14. Epub 2021 May 14.

Wellcome Centre for Integrative Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, UK.

Transforming growth factor-beta (TGF-β) family proteins mediate many vital biological functions in growth, development and regulation of the immune system. TGF-β itself controls immune homeostasis and inflammation, including conversion of naïve CD4 T cells into Foxp3 regulatory T cells (Tregs) in the presence of IL-2 and T cell receptor ligands. The helminth parasite Heligmosomoides polygyrus exploits this pathway through a structurally novel TGF-β mimic (Hp-TGM), which binds to mammalian TGF-β receptors and induces Tregs. Read More

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Tissue-Resident Memory-Like CD8 T Cells Exhibit Heterogeneous Characteristics in Tuberculous Pleural Effusion.

J Immunol Res 2021 22;2021:6643808. Epub 2021 Apr 22.

Institute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, China.

Tissue-resident memory T (T) cells are well known to play critical roles in peripheral tissues during virus infection and tumor immunology. Our previous studies indicated that CD69CD4 and CD69CD8 T cells in tuberculous pleural effusion (TPE) were antigen-specific memory T cells. However, the phenotypical and functional characteristics of CD8 T cells in tuberculosis remain unknown. Read More

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The Role of CD4 Resident Memory T Cells in Local Immunity in the Mucosal Tissue - Protection Versus Pathology.

Front Immunol 2021 21;12:616309. Epub 2021 Apr 21.

Department of Immunology, Graduate School of Medicine, Chiba University, Chiba, Japan.

Memory T cells are crucial for both local and systemic protection against pathogens over a long period of time. Three major subsets of memory T cells; effector memory T (T) cells, central memory T (T) cells, and tissue-resident memory T (T) cells have been identified. The most recently identified subset, T cells, is characterized by the expression of the C-type lectin CD69 and/or the integrin CD103. Read More

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Fli1 deficiency suppresses RALDH1 activity of dermal dendritic cells and related induction of regulatory T cells: a possible role in scleroderma.

Arthritis Res Ther 2021 05 8;23(1):137. Epub 2021 May 8.

Department of Dermatology, University of Tokyo Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.

Background: Aldehyde dehydrogenase 1 family member A1 (RALDH1)-producing dermal dendritic cells (DCs), a conventional DC subset regulating skin fibrosis, are decreased in the involved skin of patients with systemic sclerosis (SSc). In this study, we investigated the contribution of Fli1 deficiency, a potential predisposing factor of SSc, to the phenotypical alteration of RALDH1-producing dermal DCs by using SSc model mice and SSc skin samples.

Methods: Bleomycin (BLM)-induced skin fibrosis was generated with Fli1 and wild-type mice. Read More

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Single-cell Profiles and Prognostic Impact of Tumor-Infiltrating Lymphocytes Coexpressing CD39, CD103, and PD-1 in Ovarian Cancer.

Clin Cancer Res 2021 May 7. Epub 2021 May 7.

Deeley Research Centre, BC Cancer, Victoria, British Columbia, Canada.

Purpose: Tumor-infiltrating lymphocytes (TIL) are strongly associated with survival in most cancers; however, the tumor-reactive subset that drives this prognostic effect remains poorly defined. CD39, CD103, and PD-1 have been independently proposed as markers of tumor-reactive CD8 TIL in various cancers. We evaluated the phenotype, clonality, and prognostic significance of TIL expressing various combinations of these markers in high-grade serous ovarian cancer (HGSC), a malignancy in need of more effective immunotherapeutic approaches. Read More

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The human memory T cell compartment changes across tissues of the female reproductive tract.

Mucosal Immunol 2021 May 5. Epub 2021 May 5.

Fred Hutchinson Cancer Research Center, Vaccine and Infectious Disease Division, Seattle, WA, USA.

Memory CD4 T cells in tissues fulfill numerous functions that are critical for local immune homeostasis and protection against pathogens. Previous studies have highlighted the phenotypic and functional heterogeneity of circulating and tissue-resident memory CD4 T cells across different human tissues such as skin, lung, liver, and colon. Comparatively little is known in regard to memory CD4 T cells across tissues of the female reproductive tract (FRT). Read More

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CCR2 Regulates Vaccine-Induced Mucosal T-Cell Memory to Influenza A Virus.

J Virol 2021 May 5. Epub 2021 May 5.

Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, 53706, WI, USA

Elicitation of lung tissue-resident memory CD8 T cells (Ts) is a goal of T-cell based vaccines against respiratory viral pathogens, such as influenza A virus (IAV). Chemokine receptor 2 (CCR2)-dependent monocyte trafficking plays an essential role in the establishment of CD8 Ts in lungs of IAV-infected mice. Here, we used a combination adjuvant-based subunit vaccine strategy that evokes multifaceted (T1/T17/T1/T17) IAV nucleoprotein-specific lung Ts, to determine whether CCR2 and monocyte infiltration are essential for vaccine-induced T development and protective immunity to IAV in lungs. Read More

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Age-dependency of terminal ileum tissue resident memory T cell responsiveness profiles to S. Typhi following oral Ty21a immunization in humans.

Immun Ageing 2021 Apr 19;18(1):19. Epub 2021 Apr 19.

Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, 21201, USA.

Background: The impact of aging on the immune system is unequivocal and results in an altered immune status termed immunosenescence. In humans, the mechanisms of immunosenescence have been examined almost exclusively in blood. However, most immune cells are present in tissue compartments and exhibit differential cell (e. Read More

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Antigen-Specific Tissue-Resident Memory T Cells in the Respiratory System Were Generated following Intranasal Vaccination of Mice with BCG.

J Immunol Res 2021 27;2021:6660379. Epub 2021 Mar 27.

Institute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.

Tissue-resident memory T cells (T) are different from effector memory T cells (T) and central memory T cells (T) and contribute to the protective immunity against local challenges. Currently, we found that CD4 and CD8 T cells in the nasal mucosa, trachea, lungs, and lavage fluids were heterogeneous on the expression of CD69 and CD103 as well as the production of cytokines including IFN-, IL-2, and TNF-. After intranasal vaccination of mice with BCG, respiratory tissues expressed higher levels of the chemokine CXCL16 and T cells expressed CXCR6 to CXCL16. Read More

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PD-1 Expression Defines Epidermal CD8CD103 T Cells Preferentially Producing IL-17A and Using Skewed TCR Repertoire in Psoriasis.

J Invest Dermatol 2021 Apr 9. Epub 2021 Apr 9.

Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu, Japan; Allergic Disease Research Center, Chutoen General Medical Center, Kakegawa, Japan. Electronic address:

In psoriasis, CD8CD103 memory T cells residing in the epidermis represent an effector population capable of maintaining the condition and driving a recurrence of the disease. Tissue-infiltrating CD8 T cells expressing PD-1 are regarded as antigen-primed effector cells in others chronic inflammatory diseases. However, the expression and significance of PD-1 on skin-infiltrating CD8 T cells in human psoriasis is not known. Read More

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CCR2 Regulates Vaccine-Induced Mucosal T-Cell Memory to Influenza A Virus.

bioRxiv 2021 Mar 25. Epub 2021 Mar 25.

Elicitation of lung tissue-resident memory CD8 T cells (T s) is a goal of T-cell based vaccines against respiratory viral pathogens such as influenza A virus (IAV). Chemokine receptor 2 (CCR2)-dependent monocyte trafficking plays an essential role in the establishment of CD8 T s in lungs of IAV-infected mice. Here, we used a combination adjuvant-based subunit vaccine strategy that evokes multifaceted (T 1/T 17/T 1/T 17) IAV nucleoprotein-specific lung T s, to determine whether CCR2 and monocyte infiltration are essential for vaccine-induced T development and protective immunity to IAV in lungs. Read More

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Tertiary lymphoid structures show infiltration of effective tumor-resident T cells in gastric cancer.

Cancer Sci 2021 May 1;112(5):1746-1757. Epub 2021 Apr 1.

Department of Gastroenterological Surgery, Osaka City University Graduate School of Medicine, Osaka, Japan.

Several studies have reported that tissue-resident memory T cells (TRM cells) or tertiary lymphoid structures (TLSs) are associated with a good prognosis. The aim of this study was to clarify the association of TRM cells and TLSs in the tumor immune microenvironment in gastric cancer (GC). We performed immunohistochemical and immunofluorescence staining to detect the presence of CD103 T cells and to assess the association between CD103 T cells and TLSs. Read More

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Expression of MAdCAM-1 and gut-homing T-cells in inflamed pouch mucosa.

J Crohns Colitis 2021 Mar 2. Epub 2021 Mar 2.

Department of Gastroenterology and Hepatology, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands.

Background And Aims: Pouchitis is a common complication following formation of an ileal pouch-anal anastomosis (IPAA) after proctocolectomy for ulcerative colitis (UC). Gut-specific lymphocyte trafficking mechanisms have been identified as players in the pathogenesis of UC. In the present study, we aimed to characterize the presence of lymphocyte subsets expressing gut-homing molecules in pouches and peripheral blood of UC patients with and without pouchitis. Read More

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γδ T Cells in Merkel Cell Carcinomas Have a Proinflammatory Profile Prognostic of Patient Survival.

Cancer Immunol Res 2021 Mar 5. Epub 2021 Mar 5.

Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia.

Merkel cell carcinomas (MCC) are immunogenic skin cancers associated with viral infection or UV mutagenesis. To study T-cell infiltrates in MCC, we analyzed 58 MCC lesions from 39 patients using multiplex-IHC/immunofluorescence (m-IHC/IF). CD4 or CD8 T cells comprised the majority of infiltrating T lymphocytes in most tumors. Read More

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Circulating CD103 γδ and CD8 T cells are clonally shared with tissue-resident intraepithelial lymphocytes in celiac disease.

Mucosal Immunol 2021 Mar 2. Epub 2021 Mar 2.

K. G. Jebsen Centre for Coeliac Disease Research, University of Oslo, Oslo, Norway.

Gut intraepithelial γδ and CD8 αβ T lymphocytes have been connected to celiac disease (CeD) pathogenesis. Based on the previous observation that activated (CD38), gut-homing (CD103) γδ and CD8 αβ T cells increase in blood upon oral gluten challenge, we wanted to shed light on the pathogenic involvement of these T cells by examining the clonal relationship between cells of blood and gut during gluten exposure. Of 20 gluten-challenged CeD patients, 8 and 10 had increase in (CD38CD103) γδ and CD8 αβ T cells, respectively, while 16 had increase in gluten-specific CD4 T cells. Read More

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Longitudinal Immune Profiling Reveals Unique Myeloid and T-cell Phenotypes Associated with Spontaneous Immunoediting in a Prostate Tumor Model.

Cancer Immunol Res 2021 May 26;9(5):529-541. Epub 2021 Feb 26.

Columbia Center for Translational Immunology (CCTI), Columbia University Irving Medical Center, New York, New York.

The theory of cancer immunoediting, which describes the dynamic interactions between tumors and host immune cells that shape the character of each compartment, is foundational for understanding cancer immunotherapy. Few models exist that facilitate in-depth study of each of the three canonical phases of immunoediting: elimination, equilibrium, and escape. Here, we utilized NPK-C1, a transplantable prostate tumor model that we found recapitulated the three phases of immunoediting spontaneously in immunocompetent animals. Read More

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Neoadjuvant anti-OX40 (MEDI6469) therapy in patients with head and neck squamous cell carcinoma activates and expands antigen-specific tumor-infiltrating T cells.

Nat Commun 2021 02 16;12(1):1047. Epub 2021 Feb 16.

Earle A. Chiles Research Institute, Providence Cancer Institute, Portland, OR, USA.

Despite the success of checkpoint blockade in some cancer patients, there is an unmet need to improve outcomes. Targeting alternative pathways, such as costimulatory molecules (e.g. Read More

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February 2021

Modulation of gut immune response by probiotic fermented milk consumption to control IgE in a respiratory allergy model.

Benef Microbes 2021 Apr 12;12(2):175-186. Epub 2021 Feb 12.

Cátedra de Inmunología, Instituto de Microbiología, Facultad de Bioquímica, Química y Farmacia, UNT, Ayacucho 471, 4000 Tucumán, Argentina.

Allergies are a world increasing health issue and most treatments are oriented to alleviate symptoms. Probiotics have several health benefits including the improvement of the immune system. In previous work we found that consumption of commercial probiotic fermented milk (PFM) significantly reduced specific-immunoglobulin (Ig) E in serum and lungs by increasing specific-IgG and controlled allergic response to ovalbumin (OVA) in an adult mouse respiratory allergy model. Read More

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Sublingual Immunotherapy: How Sublingual Allergen Administration Heals Allergic Diseases; Current Perspective about the Mode of Action.

Pathogens 2021 Feb 2;10(2). Epub 2021 Feb 2.

Laboratory of Allergy and Immunology, The Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan.

Owing to the successful application of sublingual immunotherapy (SLIT), allergen immunotherapy (AIT) has become one of the leading treatments for allergic diseases. Similar to the case with other AITs, such as subcutaneous and oral immunotherapies, not only the alleviation of allergic symptoms, but also the curing of the diseases can be expected in patients undergoing SLIT. However, how and why such strong efficacy is obtained by SLIT, in which allergens are simply administered under the tongue, is not clearly known. Read More

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February 2021

CD8 and CD4 T Cell Populations in Human Kidneys.

Cells 2021 Feb 1;10(2). Epub 2021 Feb 1.

Department of Experimental Immunology, Amsterdam institute for Infection & Immunity, Amsterdam UMC, University of Amsterdam, 1105AZ Amsterdam, The Netherlands.

Background: At border sites, and in internal organs, tissue resident memory T cells (T contribute to the immune barrier against pathogens like viruses, bacteria, fungi, and cancer. However, information on the presence and function of these cells in the human kidney is scant. In order to better understand the T cell-mediated immunological defense in this organ, we aimed to determine phenotypic and functional aspects of CD8 and CD4 T cells present in healthy and allograft kidney tissue. Read More

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February 2021

Natural Protection From Type 1 Diabetes in NOD Mice Is Characterized by a Unique Pancreatic Islet Phenotype.

Diabetes 2021 Apr 2;70(4):955-965. Epub 2021 Feb 2.

Division of Infection and Immunity, Cardiff University School of Medicine, Cardiff, U.K.

The NOD mouse develops spontaneous type 1 diabetes, with some features of disease that are very similar to the human disease. However, a proportion of NOD mice are naturally protected from developing diabetes, and currently, studies characterizing this cohort are very limited. Here, using both immunofluorescence and multiparameter flow cytometry, we focus on the pancreatic islet morphology and immune infiltrate observed in naturally protected NOD mice. Read More

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Differential Changes in Inflammatory Mononuclear Phagocyte and T-Cell Profiles within Psoriatic Skin during Treatment with Guselkumab vs. Secukinumab.

J Invest Dermatol 2021 Jan 30. Epub 2021 Jan 30.

Immunoregulation Laboratory, Research Centre of University of Montreal Hospital, Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montreal, Quebec, Canada. Electronic address:

Cellular sources of IL-23 and IL-17A driving skin inflammation in psoriasis remain unclear. Using high-dimensional unsupervised flow cytometry analysis, mononuclear phagocytes and T cells were examined in the same lesions of patients before and during guselkumab (IL-23p19 blocker) or secukinumab (IL-17A blocker) treatment. Among CD11cHLA-DR mononuclear phagocytes, CD64CD163CD14CD1cCD1a inflammatory monocyte‒like cells were the predominant IL-23-producing cells and, together with CD64CD163CD14IL-23p19TNF-α inflammatory dendritic cell‒like cells, were increased in lesional compared with those in nonlesional skin taken from the same patient. Read More

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January 2021

Human intestinal tissue-resident memory T cells comprise transcriptionally and functionally distinct subsets.

Cell Rep 2021 Jan;34(3):108661

Translational Gastroenterology Unit, Nuffield Department of Medicine, University of Oxford, Oxford OX3 9DU, UK; Oxford Transplant Centre, Churchill Hospital, Oxford University Hospitals NHS Foundation Trust, Oxford OX3 7LE, UK; NIHR Biomedical Research Centre, John Radcliffe Hospital, Oxford University Hospitals NHS Foundation Trust, Oxford OX3 9DU, UK.

Tissue-resident memory T (T) cells provide key adaptive immune responses in infection, cancer, and autoimmunity. However, transcriptional heterogeneity of human intestinal T cells remains undefined. Here, we investigate transcriptional and functional heterogeneity of human T cells through study of donor-derived T cells from intestinal transplant recipients. Read More

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January 2021

Tissue-Resident Memory CD8+ T Cells From Skin Differentiate Psoriatic Arthritis From Psoriasis.

Arthritis Rheumatol 2021 Jan 16. Epub 2021 Jan 16.

University Medical Center Utrecht, Utrecht, The Netherlands.

Objective: To compare immune cell phenotype and function in psoriatic arthritis (PsA) versus psoriasis in order to better understand the pathogenesis of PsA.

Methods: In-depth immunophenotyping of different T cell and dendritic cell subsets was performed in patients with PsA, psoriasis, or axial spondyloarthritis and healthy controls. Subsequently, we analyzed cells from peripheral blood, synovial fluid (SF), and skin biopsy specimens using flow cytometry, along with high-throughput transcriptome analyses and functional assays on the specific cell populations that appeared to differentiate PsA from psoriasis. Read More

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January 2021