37 results match your criteria caspases mcs

Maize metacaspases modulate the defense response mediated by the NLR protein Rp1-D21 likely by affecting its subcellular localization.

Plant J 2021 01 20;105(1):151-166. Epub 2020 Nov 20.

The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, Shandong, 266237, PR China.

Plants usually employ resistance (R) genes to defend against the infection of pathogens, and most R genes encode intracellular nucleotide-binding, leucine-rich repeat (NLR) proteins. The recognition between R proteins and their cognate pathogens often triggers a rapid localized cell death at the pathogen infection sites, termed the hypersensitive response (HR). Metacaspases (MCs) belong to a cysteine protease family, structurally related to metazoan caspases. Read More

View Article and Full-Text PDF
January 2021

Characterization of microcystin-induced apoptosis in HepG2 hepatoma cells.

Toxicon 2020 Jan 14;173:20-26. Epub 2019 Nov 14.

Biological Science Department, Kent State University, Kent, OH 44242, United States. Electronic address:

Microcystins (MCs) are a class of hepatotoxins that are commonly produced by freshwater cyanobacteria. MCs harm liver cells through inhibiting protein phosphatases 1 and 2A (PP1 and PP2A) and can produce dualistic effects, i.e. Read More

View Article and Full-Text PDF
January 2020

Type-II Metacaspases Mediate the Processing of Plant Elicitor Peptides in Arabidopsis.

Mol Plant 2019 11 24;12(11):1524-1533. Epub 2019 Aug 24.

State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. Electronic address:

Plants can produce animal cytokine-like immune peptides, among which plant elicitor peptides (Peps) derive from the C termini of their precursors (PROPEPs). Recently, the functions of Peps have been expanded beyond plant immunity. However, a long-standing enigma is how PROPEPs are processed into Peps. Read More

View Article and Full-Text PDF
November 2019

Genome-Wide Identification and Expression Analysis of the Metacaspase Gene Family in Species.

Genes (Basel) 2019 07 12;10(7). Epub 2019 Jul 12.

State Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton, The Ministry of Agriculture, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China.

Metacaspases (MCs) are cysteine proteases that are important for programmed cell death (PCD) in plants. In this study, we identified 89 genes in the genomes of four species (, , , and ), and classified them as type-I or type-II genes. All of the type-I and type-II genes contain a sequence encoding the peptidase C14 domain. Read More

View Article and Full-Text PDF

Genome-wide identification of barley MCs (metacaspases) and their possible roles in boron-induced programmed cell death.

Mol Biol Rep 2018 Jun 5;45(3):211-225. Epub 2018 Feb 5.

Department of Genetics Research, Institute for Research and Medical Consultations, Imam Abdulrahman Bin Faisal University, P. O. Box 1982, Dammam, 31441, Saudi Arabia.

Developmental processes and stress-induction activate many key proteins in plants such as metacaspase which regulate programmed cell death (PCD). In this study, identification of barley metacaspases and their possible roles upon boron (B)-induction was investigated by using in silico and wet-lab methods. Genome-wide analysis revealed that barley genome harbor ten metacaspases which divided into three groups: Type-I, -I* and -II. Read More

View Article and Full-Text PDF

The function of two type II metacaspases in woody tissues of Populus trees.

New Phytol 2018 03 15;217(4):1551-1565. Epub 2017 Dec 15.

Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, 90187, Umeå, Sweden.

Metacaspases (MCs) are cysteine proteases that are implicated in programmed cell death of plants. AtMC9 (Arabidopsis thaliana Metacaspase9) is a member of the Arabidopsis MC family that controls the rapid autolysis of the xylem vessel elements, but its downstream targets in xylem remain uncharacterized. PttMC13 and PttMC14 were identified as AtMC9 homologs in hybrid aspen (Populus tremula × tremuloides). Read More

View Article and Full-Text PDF

Decreased Poly(ADP-Ribose) Polymerase 1 Expression Attenuates Glucose Oxidase-Induced Damage in Rat Cochlear Marginal Strial Cells.

Mol Neurobiol 2016 11 2;53(9):5971-5984. Epub 2015 Nov 2.

Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan, 430022, China.

Oxidative damage to the inner ear is responsible for several types of sensorineural deafness. Cochlear stria marginal cells (MCs) are thought to be vulnerable to such oxidative stress. Activated poly(ADP-ribose) polymerase 1 (PARP1) has been implicated in several diseases, but the effect of PARP1 on MCs subjected to oxidative stress remains elusive. Read More

View Article and Full-Text PDF
November 2016

Contribution of mast cells to injury mechanisms in a mouse model of pediatric traumatic brain injury.

J Neurosci Res 2016 12 10;94(12):1546-1560. Epub 2016 Sep 10.

PROTECT, INSERM, Unversité Paris Diderot, Sorbonne Paris Cité, Paris, France.

The cognitive and behavioral deficits caused by traumatic brain injury (TBI) to the immature brain are more severe and persistent than injuries to the adult brain. Understanding this developmental sensitivity is critical because children under 4 years of age of sustain TBI more frequently than any other age group. One of the first events after TBI is the infiltration and degranulation of mast cells (MCs) in the brain, releasing a range of immunomodulatory substances; inhibition of these cells is neuroprotective in other types of neonatal brain injury. Read More

View Article and Full-Text PDF
December 2016

Microcystin-LR Induced Apoptosis in Rat Sertoli Cells via the Mitochondrial Caspase-Dependent Pathway: Role of Reactive Oxygen Species.

Front Physiol 2016 9;7:397. Epub 2016 Sep 9.

Department of Environmental Health, School of Public Health, Zhengzhou University Zhengzhou, China.

Microcystins (MCs), the secondary metabolites of blue-green algae, are ubiquitous and major cyanotoxin contaminants. Besides the hepatopancreas/liver, the reproductive system is regarded as the most important target organ for MCs. Although reactive oxygen species (ROS) have been implicated in MCs-induced reproductive toxicity, the role of MCs in this pathway remains unclear. Read More

View Article and Full-Text PDF
September 2016

PEDF counteracts DL-α-aminoadipate toxicity and rescues gliotoxic damages in RPE-free chicken retinal explants.

Exp Eye Res 2015 May 14;134:111-22. Epub 2015 Feb 14.

Technische Universität Darmstadt, Entwicklungsbiologie und Neurogenetik, Schnittspahnstrasse 13, D-64287 Darmstadt, Germany. Electronic address:

Gliotoxic responses complicate human eye diseases, the causes of which often remain obscure. Here, we activated Müller cells (MCs) by the gliotoxin DL-α-aminoadipate (AAA) and assayed possible protective effects by pigment epithelium-derived factor (PEDF) in RPE-free retinal explants of the E6 chick embryo. These models are suited to analyze gliotoxic reactions in vitro, since the avian retina contains only Müller cells (MCs) as glial components, and the RPE-free explants are devoid of a major PEDF source. Read More

View Article and Full-Text PDF

Comparison of 2D- and 3D-culture models as drug-testing platforms in breast cancer.

Oncol Rep 2015 Apr 29;33(4):1837-43. Epub 2015 Jan 29.

Division of Medical Oncology/Hematology, Department of Medicine, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan.

It is becoming recognized that screening of oncology drugs on a platform using two-dimensionally (2D)-cultured cell lines is unable to precisely select clinically active drugs; therefore three-dimensional (3D)-culture systems are emerging and show potential for better simulating the in vivo tumor microenvironment. The purpose of this study was to reveal the differential effects of chemotherapeutic drugs between 2D- and 3D-cultures and to explore their underlying mechanisms. We evaluated differences between 2D- and 3D-cultured breast cancer cell lines by assessing drug sensitivity, oxygen status and expression of Ki-67 and caspases. Read More

View Article and Full-Text PDF

CD39 is a negative regulator of P2X7-mediated inflammatory cell death in mast cells.

Cell Commun Signal 2014 Jul 16;12:40. Epub 2014 Jul 16.

Background: Mast cells (MCs) are major contributors to an inflammatory milieu. One of the most potent drivers of inflammation is the cytokine IL-1β, which is produced in the cytoplasm in response to danger signals like LPS. Several controlling mechanisms have been reported which limit the release of IL-1β. Read More

View Article and Full-Text PDF

Genomewide survey and characterization of metacaspase gene family in rice (Oryza sativa).

J Genet 2014 Apr;93(1):93-102

National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070 Wuhan, People's Republic of China.

Metacaspases (MCs), which are cysteine-dependent proteases found in plants, fungi, and protozoa, may be involved in programmed cell death processes, being distant relatives of metazoan caspases. In this study, we analysed the structures, phylogenetic relationship, genome localizations, expression patterns and domestic selections of eight MC genes identified in rice (OsMC). Alignment analysis of the corresponding protein sequences suggested OsMC proteins can be classified into two subtypes. Read More

View Article and Full-Text PDF

Secretory lysosomes of mouse mast cells store and exocytose active caspase-3 in a strictly granzyme B dependent manner.

Eur J Immunol 2013 Dec;43(12):3209-18

Institute of Biochemistry and Molecular Immunology, University Clinic, RWTH Aachen University, Aachen, Germany.

In this study, we report that cytoplasmic granules from in vivo and in vitro derived mouse mast cells (MCs) contain active granzyme B (gzmB) and caspase-3, which is consistent with recent findings. Studying WT and gzmB-deficient mice, we observed that BM-derived MCs (BMMCs) from both strains contain cytosolic pro-caspase-3, but only WT BMMCs expressed active caspase-3 limited to their secretory lysosomes. Confocal microscopy revealed colocalization of active caspase-3 and gzmB in these cytoplasmic granules. Read More

View Article and Full-Text PDF
December 2013

Female zebrafish (Danio rerio) are more vulnerable than males to microcystin-LR exposure, without exhibiting estrogenic effects.

Aquat Toxicol 2013 Oct 15;142-143:272-82. Epub 2013 Jul 15.

Fisheries College of Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, People's Republic of China.

Microcystins (MCs) released during cyanobacterial blooms exert varied toxicity on fish. Up to now, the reproductive toxicity of MCs on fish has rarely been reported. The present study investigated the reproductive toxicity of microcystin-LR (MC-LR) on male and female zebrafish (Danio rerio) by subchronic immersion in 1, 5, 20 μg/L for 30 d. Read More

View Article and Full-Text PDF
October 2013

Spironolactone inhibits apoptosis in rat mesangial cells under hyperglycaemic conditions via the Wnt signalling pathway.

Mol Cell Biochem 2013 Aug 29;380(1-2):185-93. Epub 2013 Apr 29.

Department of Nephrology, The First Affiliated Hospital of Harbin Medical University, Heilongjiang 150001, China.

Mesangial cells (MCs) play a crucial role in maintaining structure and function of glomerular tufts, providing structural support for capillary loops and modulating glomerular filtration by their contractility. MCs apoptosis occurs in experimental diabetic nephropathy, and this correlates with worsening albuminuria. Accumulating evidence suggests that mineralocorticoid receptor (MR) blockade effectively reduces proteinuria in diabetic nephropathy; however, it is rarely known whether spironolactone (SPI), a nonspecific MR antagonist, inhibits apoptosis in MCs under hyperglycaemic conditions. Read More

View Article and Full-Text PDF

Oxidant stress and mitochondrial signaling regulate reversible changes of ERα expression and apoptosis in aging mouse glomeruli and mesangial cells.

Endocrinology 2012 Nov 1;153(11):5491-9. Epub 2012 Oct 1.

Laboratory on Sex and Gender Differences in Health and Disease, Department of Surgery, Miller School of Medicine, University of Miami, Florida 33136, USA.

Estrogen actions are largely dependent on the intracellular estrogen receptor (ER) levels. During aging the decline of estrogens or ER leads to a loss in antiinflammatory protection and an increase in oxidant stress due to changes in mitochondrial function. Estrogens/ER may also coordinate signaling between the nucleus and mitochondria through ERK activation, which paradoxically decreases ER expression. Read More

View Article and Full-Text PDF
November 2012

Critical role for mast cells in interleukin-1β-driven skin inflammation associated with an activating mutation in the nlrp3 protein.

Immunity 2012 Jul 19;37(1):85-95. Epub 2012 Jul 19.

Department of Pathology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Cryopyrin-associated periodic syndromes (CAPS) are caused by aberrant interleukin-1β (IL-1β) production induced by mutations in the NLRP3 protein in humans, but the mechanisms involved remain poorly understood. Using a mouse model, we show a role for the indigenous microbiota and mast cells (MCs) in skin disease associated with mutant Nlrp3 protein. Unlike normal cells, MCs expressing mutant Nlrp3 produced IL-1β in response to lipopolysaccharide or tumor necrosis factor-α (TNF-α). Read More

View Article and Full-Text PDF

Toxic effects of microcystin-LR on the HepG2 cell line under hypoxic and normoxic conditions.

J Appl Toxicol 2013 Oct 27;33(10):1180-6. Epub 2012 Apr 27.

Fisheries College of Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.

Microcystins (MCs) are highly liver-specific and evidenced as a liver tumor promoter. Oxidative stress is one of the most important toxicity mechanisms of MCs, which is tightly related to oxygen concentration. The effects of MCs on animals and cell lines in normoxia and the mechanisms have been well studied, but such effects in different oxygen conditions were still unclear. Read More

View Article and Full-Text PDF
October 2013

Apoptotic responses of zebrafish (Danio rerio) after exposure with microcystin-LR under different ambient temperatures.

J Appl Toxicol 2013 Aug 9;33(8):799-806. Epub 2012 Mar 9.

Fisheries College of Huazhong Agricultural University, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Wuhan, 430070, PR China.

Microcystins (MCs) can cause evident hepatic apoptosis. In vitro studies indicated that uptake of MC by isolated hepatocytes was dramatically reduced as ambient temperature dropped, and some studies presented a hypothesis that differences in core body temperatures in animals result in diverse uptake of MC, as well as different toxic effects. Thus far, however, few in vivo studies have been conducted to investigate the effects of temperature on MC-induced hepatocyte apoptosis in fish, a typical poikilotherm. Read More

View Article and Full-Text PDF

Microcystin-LR causes cytotoxicity effects in rat testicular Sertoli cells.

Yan Li Xiaodong Han

Environ Toxicol Pharmacol 2012 Mar 29;33(2):318-26. Epub 2011 Dec 29.

Life Science School, Nanjing University, Nanjing, Jiangsu 210093, PR China.

Microcystins (MCs) are produced by cyanobacteria. The most toxic and widely distributed MC is microcystin-LR (MC-LR). The aim of this study was to investigate whether exposure to MC-LR could induce oxidative stress, leading the further toxicity effects on Sertoli cells in vitro. Read More

View Article and Full-Text PDF

Increased glyoxalase I levels inhibit accumulation of oxidative stress and an advanced glycation end product in mouse mesangial cells cultured in high glucose.

Exp Cell Res 2012 Jan 21;318(2):152-9. Epub 2011 Oct 21.

Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine (KIOM), 483 Exporo, Yuseong-gu, Daejeon 305-811, South Korea.

Chronic high glucose levels lead to the formation of advanced glycation end-products (AGEs) as well as AGE precursors, such as methylglyoxal (MG) and glyoxal, via non-enzymatic glycation reactions in patients with diabetic mellitus. Glyoxalase 1 (GLO-1) detoxifies reactive dicarbonyls that form AGEs. To investigate the interaction between AGEs and GLO-1 in mesangial cells (MCs) under diabetic conditions, AGE levels and markers of oxidative stress were measured in GLO-1-overexpressing MCs (GLO-1-MCs) cultured in high glucose. Read More

View Article and Full-Text PDF
January 2012

[The recombinant adeno-associated viruses of the serotypes 2 mediated overexpression of MnSOD protects against oxidation-induced apoptosis in stria marginal cells of rat cochlea].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2011 May;25(10):463-8

Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 400022, China.

Objective: To investigate the influence of overexpression of manganese superoxide dismutase (MnSOD) of stria marginal cells (MCs) of the rat cochlea by the recombinant adeno-associated viruses of the serotypes 2 (AAV2) mediated gene-delivery for hydrogen peroxide-induced oxidative stress in vitro.

Method: Primary cultures of MCs were infected using rAAV2-MnSOD-EGFP at dosage of multiplicity of infection (MOI) 10(1)v x /cell and using rAAV2-EGFP as control. The expression of MnSOD in MCs was examined using western blot and the activity of MnSOD was determinated by colorimetric assays. Read More

View Article and Full-Text PDF

Cell cycle arrest and cytochrome c-mediated apoptotic induction by MCS-5A is associated with up-regulation of p16(INK4a) in HL-60 cells.

Bioorg Med Chem Lett 2010 Jul 15;20(13):3880-4. Epub 2010 May 15.

Department of Molecular Biology & Pharmacology, Kyunggi-Do, Republic of Korea.

MCS-5A, an analog of sangivamycin, selectively inhibits the cyclin-dependent kinases CDK1 and 4 in HL-60 cells in vitro (IC(50): 9.6 and 8.8 1V, respectively), while weakly inhibiting other housekeeping protein kinases. Read More

View Article and Full-Text PDF

H1-receptor antagonists terfenadine and loratadine inhibit spontaneous growth of neoplastic mast cells.

Exp Hematol 2010 Oct 1;38(10):896-907. Epub 2010 Jun 1.

Department for Companion Animals and Horses, Clinic for Internal Medicine and Infectious Diseases, University of Veterinary Medicine Vienna, Vienna, Austria.

Objective: In mast cell (MC) neoplasms, clinical problems requiring therapy include local aggressive and sometimes devastating growth of MCs and mediator-related symptoms. A key mediator of MCs responsible for clinical symptoms is histamine. Therefore, use of histamine receptor (HR) antagonists is an established approach to block histamine effects in these patients. Read More

View Article and Full-Text PDF
October 2010

Involvement of Fas/FasL system in apoptotic signaling in testicular germ cells of male Wistar rats injected i.v. with microcystins.

Toxicon 2009 Jul;54(1):1-7

Donghu Experimental Station of Lake Ecosystems, State Key Laboratory for Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, People's Republic of China.

Previous studies have shown that gonads were the second target organ of microcystins (MCs), and that MCs exposure exerted obvious toxic effects on male reproductive system of mammals. However, relevant molecular evidences are still lacking. Fas-signaling pathway plays a key role in toxicant-induced germ cell apoptosis. Read More

View Article and Full-Text PDF

Mast cells are early responders after hypoxia-ischemia in immature rat brain.

Stroke 2009 Sep 11;40(9):3107-12. Epub 2009 Jun 11.

Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY, USA.

Background And Purpose: Perinatal hypoxia-ischemia (HI) produces acute and prolonged inflammation of the brain. Mast cells (MCs), numerous in the pia and CNS of neonatal rats, can initiate inflammation attributable to preformed mediators. MCs contribute to HI brain damage in the neonatal rat; MC stabilization protects through 48 hours of reperfusion. Read More

View Article and Full-Text PDF
September 2009

Mast cells mediate neutrophil recruitment and vascular leakage through the NLRP3 inflammasome in histamine-independent urticaria.

J Exp Med 2009 May 13;206(5):1037-46. Epub 2009 Apr 13.

Department of Dermatology, Chiba University Graduate School of Medicine, Chuo-ku, Chiba 260-8670, Japan.

Urticarial rash observed in cryopyrin-associated periodic syndrome (CAPS) caused by nucleotide-binding oligomerization domain-leucine-rich repeats containing pyrin domain 3 (NLRP3) mutations is effectively suppressed by anti-interleukin (IL)-1 treatment, suggesting a pathophysiological role of IL-1beta in the skin. However, the cellular mechanisms regulating IL-1beta production in the skin of CAPS patients remain unclear. We identified mast cells (MCs) as the main cell population responsible for IL-1beta production in the skin of CAPS patients. Read More

View Article and Full-Text PDF

Proteomic and phosphoproteomic analysis of cellular responses in medaka fish (Oryzias latipes) following oral gavage with microcystin-LR.

Toxicon 2008 Jun 25;51(8):1431-9. Epub 2008 Mar 25.

USM 505/EA 4105, Département de Régulations, Développement et Diversité Moléculaire, Ecosystèmes et Interactions Toxiques, Muséum National d'Histoire Naturelle, 12 Rue Buffon, Paris Cedex 05, France.

Chronic and subchronic toxicity resulting from exposure to microcystins (MCs) receives increasing attention due to the risk of bioaccumulation of these toxins by aquatic animals, including fish. The mechanisms of action of MCs that target the liver, involve modifications of protein phosphorylation resulting from phosphatases 1 and 2A inhibition. Therefore, studying phosphoprotein modifications by using a specific phosphoprotein stain Pro-Q Diamond in fish liver contaminated with MC-leucine-arginine (MC-LR), the most toxic MC, should help dissecting disturbed signaling and metabolic networks. Read More

View Article and Full-Text PDF