10,276 results match your criteria caspase-3 caspase-9

Tannic acid repair of zearalenone-induced damage by regulating the death receptor and mitochondrial apoptosis signaling pathway in mice.

Environ Pollut 2021 Jun 9;287:117557. Epub 2021 Jun 9.

Colleges of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China; Hunan Co-innovation Center of Animal Production Safety, Changsha, 410128, China. Electronic address:

Zearalenone (ZEA) is an estrogenic toxin produced by Fusarium strains, that is widely present in crops, and endangers the reproductive system of animals. Tannic acid (TA) is a natural polyphenolic substance that is widespread in the roots, stems, and leaves of plants, and has special pharmacological activity. This study was designed to investigate the therapeutic effect of TA on ZEA-induced ovarian damage in mice and to explore the molecular mechanism involved. Read More

View Article and Full-Text PDF

Luteolin-7-O-glucoside inhibits cell proliferation and modulates apoptosis through the AKT signaling pathway in human nasopharyngeal carcinoma.

Environ Toxicol 2021 Jun 24. Epub 2021 Jun 24.

Department of Otorhinolaryngology, Head and Neck Surgery, Changhua Christian Hospital, Changhua, Taiwan.

Nasopharyngeal carcinoma (NPC) is an unnoticeable malignant tumor with a high potential of lymphatic metastasis, and its prevalence is high in Asia. Ionizing radiation is the mainstay of treatment for patients with NPC without metastasis. However, patients with metastatic lesions require advanced treatments such as chemotherapy. Read More

View Article and Full-Text PDF

Bupivacaine inhibits the malignant biological behavior of oral squamous cell carcinoma cells by inhibiting the activation of ERK1/2 and STAT3.

Ann Transl Med 2021 May;9(10):839

Oral & Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou, China.

Background: Oral squamous cell carcinoma (OSCC) is an aggressive malignant tumor. Bupivacaine (Bupi), a local anesthetic drug, has been shown to display anti-tumor activity against a variety of tumors.

Methods: We selected OSCC CAL-27 cells as the model. Read More

View Article and Full-Text PDF

SOX4-induced upregulation of ARHGAP9 promotes the progression of acute myeloid leukemia.

Drug Dev Res 2021 Jun 22. Epub 2021 Jun 22.

Department of Pediatrics, Zibo Central Hospital, Zibo, China.

Acute myeloid leukemia (AML) is the most common acute leukemia. Rho GTPase activating protein 9 (ARHGAP9) has been reported to be positively correlated with overall survival of AML patients, but the specific molecular function remains unclear. This study aims to further explore the functional role and the molecular mechanism of ARHGAP9 in AML cells. Read More

View Article and Full-Text PDF

N-acetylcysteine ameliorate cytotoxic injury in piglets sertoli cells induced by zearalenone and deoxynivalenol.

Environ Sci Pollut Res Int 2021 Jun 22. Epub 2021 Jun 22.

College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei, 230036, China.

Zearalenone (ZEA) and Deoxynivalenol (DON) are two mycotoxins highly detected in agricultural products and feed. Both mycotoxins produce reproductive toxicity and pose a serious threat to human and animal health, among which pigs are the most sensitive animals. Sertoli cells (SCs) play an important role in spermatogenesis; however, the combined toxicity of ZEA and DON and the screening of effective protective agents remains to be determined. Read More

View Article and Full-Text PDF

Apoptotic mechanisms of myricitrin isolated from Madhuca longifolia leaves in HL-60 leukemia cells.

Mol Biol Rep 2021 Jun 22. Epub 2021 Jun 22.

Medicinal Chemistry and Immunology Lab (ASK-II-406), School of Chemical and Biotechnology (SCBT), SASTRA Deemed University, Thanjavur, India.

Myricitrin, a naturally occurring flavonoid in Madhuca longifolia, possesses several medicinal properties. Even though our earlier work revealed its role against the proliferation of acute myelogenous leukemia cells (HL-60), its molecular mechanisms have not yet been revealed. The current study aims to explore the molecular mechanisms of myricitrin (isolated from an ethnomedicinal drug Madhuca longifolia) to induce apoptosis in HL-60 cells. Read More

View Article and Full-Text PDF

Grape seed proanthocyanidin extract ameliorates cisplatin-induced testicular apoptosis via PI3K/Akt/mTOR and endoplasmic reticulum stress pathways in rats.

J Food Biochem 2021 Jun 21:e13825. Epub 2021 Jun 21.

Department of Toxicology, School of Public Health, Lanzhou University, Lanzhou, China.

Testicular toxicity is an adverse reaction of the effective chemotherapy drug cisplatin (CIS). Our previous study found that grape seed proanthocyanidin extract (GSPE) had a protective effect on CIS-induced testicular toxicity. However, the protective mechanism of GSPE against CIS-induced testicular toxicity remains unknown. Read More

View Article and Full-Text PDF

Regulation of glial cell-derived neurotrophic factor in sevoflurane-induced neuronal apoptosis by long non-coding RNA CDKN2B-AS1 as a ceRNA to adsorb miR-133.

Am J Transl Res 2021 15;13(5):4760-4770. Epub 2021 May 15.

Department of Anesthesiology, Yanbian University Hospital (Yanbian Hospital) Yanji, Jilin Province, China.

Objective: To investigate the regulatory mechanism of sevoflurane-induced neuronal apoptosis through analyzing the expression of glial cell-derived neurotrophic factor (GDNF) mediated by miR-133, sponged by long non-coding RNA (lncRNA) CDKN2B-AS1.

Methods: An in vitro cell injury model was established by using different concentrations of sevoflurane and primary hippocampal neurons. Cell proliferation was detected by Cell Counting Kit-8 (CCK-8); caspase-3 and caspase-9 activities were detected by colorimetry, and apoptotic cells were determined by Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Read More

View Article and Full-Text PDF

Compatibility of ingredients of Danshen (Radix ) and Honghua () and their protective effects on cerebral ischemia-reperfusion injury in rats.

Exp Ther Med 2021 Aug 8;22(2):849. Epub 2021 Jun 8.

College of Life Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China.

Danshen (Radix ) and Honghua () (Danhong) are two drugs commonly prescribed together, which are often used in the treatment of cerebrovascular diseases in China. Due to the complexity of the ingredients of Danhong, the present study focused on performing the orthogonal compatibility method on the primary effective molecules of this drug: Tanshinol, salvianolic acid A, salvianolic acid B and hydroxysafflor yellow A. These four molecules were studied to determine their protective effects and to screen for the most compatible ingredients to improve cerebral ischemia-reperfusion injury (IR) in rats. Read More

View Article and Full-Text PDF

Exposure to the herbicide butachlor activates hepatic stress signals and disturbs lipid metabolism in mice.

Chemosphere 2021 Jun 15;283:131226. Epub 2021 Jun 15.

College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China. Electronic address:

Butachlor is a systemic herbicide widely applied on wheat, rice, beans, and different other crops, and is frequently detected in groundwater, surface water, and soil. Therefore, it is necessary to investigate the potential adverse health risks and the underlying mechanisms of hepatotoxicity caused by exposure to butachlor in invertebrates, other nontarget animals, and public health. For this reason, a total of 20 mice were obtained and randomly divided into two groups. Read More

View Article and Full-Text PDF

Tanshinol alleviates ulcerative colitis by promoting the expression of VLDLR.

Drug Dev Res 2021 Jun 18. Epub 2021 Jun 18.

Department of Anorectal Surgery, Wuxi Traditional Chinese Medicine Hospital, Wuxi, PR China.

Tanshinol (TAN) is a widely used Chinese medicine ingredient with anti-inflammatory activity. The therapeutic effect of TAN in ulcerative colitis (UC) deserves further investigation. DSS induced UC model for mice, and TAN of different concentrations was used for in vivo therapy. Read More

View Article and Full-Text PDF

Cellular Communication Network Factor 1 (CCN1) Knockdown Exerts a Protective Effect for Hepatic Ischemia/Reperfusion Injury by Deactivating the MEK/ERK Pathway.

Clin Res Hepatol Gastroenterol 2021 Jun 15:101737. Epub 2021 Jun 15.

Department of Anesthesiology, the First Hospital of Jilin University, Changchun, Jilin 130021, China. Electronic address:

Objective: Hepatic ischemia/reperfusion injury (IRI) is an unsettled and intractable conundrum in clinical treatment after liver transplantation and resection. Cellular communication network factor 1 (CCN1) is upregulated in liver IRI and may play a key role in this process. The objective of this study is to investigate the regulatory mechanism of CCN1 in liver IRI, which may provide new insight into liver IRI clinical treatment. Read More

View Article and Full-Text PDF

Near infrared II laser controlled free radical releasing nanogenerator for synergistic nitric oxide and alkyl radical therapy of breast cancer.

Nanoscale 2021 Jun 17. Epub 2021 Jun 17.

Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Guangdong Provincial Engineering and Technological Research Center for Drug Carrier Development, Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China.

Recently, alkyl radicals have attracted much attention in cancer therapy due to their oxygen-independent generation property. For the first time, alkyl radical and nitric oxide (NO) combined therapy is demonstrated as an effective strategy for tumor inhibition. As a proof of concept, a biocompatible free radical nanogenerator with near-infrared (NIR) II laser-induced simultaneous NO and alkyl radical release property was elaborately fabricated. Read More

View Article and Full-Text PDF

High-Dose Dexmedetomidine Promotes Apoptosis in Fetal Rat Hippocampal Neurons.

Drug Des Devel Ther 2021 8;15:2433-2444. Epub 2021 Jun 8.

Department of Anesthesiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, People's Republic of China.

Objective: Dexmedetomidine (DEX) is a potent a2-adrenoceptor agonist that has sedative, analgesic, and anxiolytic effects. Its primary clinical use is as an adjunct to general anesthesia to reduce anesthetic doses, provide analgesia and sedation in the preoperative and postoperative periods, it also used in intensive care units (ICUs). However, high concentrations of DEX may have toxic effects on neurons and cause neuronal apoptosis. Read More

View Article and Full-Text PDF

[Curcumin induces human lens epithelial cell apoptosis and cell cycle arrest by inhibiting Wnt/β-catenin signaling pathway].

Nan Fang Yi Ke Da Xue Xue Bao 2021 May;41(5):722-728

Department of Ophthalmology, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China.

Objective: To investigate the effect of curcumin on cell cycle and apoptosis of human lens epithelial cells and the possible molecular mechanism.

Objective: Cultured human lens epithelial cell line HLEC-SRA01/04 was treated with 20, 40 and 60 μmol/L curcumin for 24 or 48 h. The cell proliferation inhibition rate was determined using MTT assay, and the changes in cell cycle, mitochondrial membrane potential and apoptosis rate were analyzed with flow cytometry. Read More

View Article and Full-Text PDF

Selenium deficiency causes apoptosis through endoplasmic reticulum stress in swine small intestine.

Biofactors 2021 Jun 15. Epub 2021 Jun 15.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

Selenium (Se) plays a crucial role in intestinal health. However, the specific mechanism by which deficiency of Se causes intestinal damage remains unclear. This study was to explore whether Se deficiency can cause ER stress and induce apoptosis in swine small intestine. Read More

View Article and Full-Text PDF

Reproductive toxicity of cadmium in pubertal male rats induced by cell apoptosis.

Toxicol Ind Health 2021 Jun 15:7482337211022615. Epub 2021 Jun 15.

School of Public Health, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, 481115Wuhan University of Science and Technology, Wuhan, China.

Cadmium (Cd) is a heavy metal that is widely present in modern industrial production. It is a known, highly toxic environmental endocrine disruptor. Long-term exposure to Cd can cause varying degrees of damage to the liver, kidney, and reproductive system of organisms, especially the male reproductive system. Read More

View Article and Full-Text PDF

Protein Arginine Methyltransferase 5 Promotes Esophageal Squamous Cell Carcinoma Proliferation and Metastasis via LKB1/AMPK/mTOR Signaling Pathway.

Front Bioeng Biotechnol 2021 28;9:645375. Epub 2021 May 28.

Department of Oncology, Heze Municipal Hospital, Heze, China.

Esophageal squamous cell carcinoma (ESCC) is the eighth most common cancer in the world. Protein arginine methyltransferase 5 (PRMT5), an enzyme that catalyzes symmetric and asymmetric methylation on arginine residues of histone and non-histone proteins, is overexpressed in many cancers. However, whether or not PRMT5 participates in the regulation of ESCC remains largely unclear. Read More

View Article and Full-Text PDF

Effects of Water Extract of (Bge.) Kitag. on Different Breast Cancer Cell Lines.

Evid Based Complement Alternat Med 2021 19;2021:6665949. Epub 2021 May 19.

Department of Applied Chemistry, Chaoyang University of Technology, 168 Jifeng East Road, Taichung, Taiwan.

(Bge.) Kitag. (CP) is an important medicinal herb used in Chinese herbal medicine, with a variety of biological activities including anticancer property. Read More

View Article and Full-Text PDF

ADAM7 promotes the proliferation and invasion in trophoblast cells.

Exp Mol Pathol 2021 Jun 8;121:104659. Epub 2021 Jun 8.

Department of Motherhood, Jinan Maternity and Child Care Hospital, Jinan, Shandong, China. Electronic address:

ADAMs are members of the metzincin family of zinc-dependent metalloproteinases, which play a key role in the proteolytic degradation of the extracellular matrix to invade cells. It is well known that ADAMs are involved in regulating the invasion of trophoblast cells. But the function and underlying mechanism of ADAM7 in trophoblast cells is still unknown. Read More

View Article and Full-Text PDF

Renoprotective effects of levosimendan on acute kidney injury following cardiac arrest via anti-inflammation, anti-apoptosis and ERK activation.

FEBS Open Bio 2021 Jun 11. Epub 2021 Jun 11.

Department of Emergency, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

ATP-sensitive potassium channels (KATPs) have protective effects in ischemia-reperfusion-induced injuries and can be activated by levosimendan. This study investigated the effects of levosimendan on renal injury, inflammation, apoptosis and survival in a rat model of acute kidney injury (AKI) following cardiac arrest (CA) and cardiopulmonary resuscitation (CPR). Rats underwent a 5-minute asphyxia-based CA and resuscitation. Read More

View Article and Full-Text PDF

Celastrol induces caspase-dependent apoptosis of hepatocellular carcinoma cells by suppression of mammalian target of rapamycin.

J Tradit Chin Med 2021 Jun;41(3):381-389

The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China.

Objective: To investigate the efficacy of celastrol treatment of hepatocellular carcinoma (HCC) cells in vitro and in vivo and to propose a mechanism of action.

Methods: A human HepG2 liver cancer cell line and a xenograft tumor model were used to investigate the effects of celastrol on HCC in vitro and in vivo. A CCK-8 kit was used to detect cell viability. Read More

View Article and Full-Text PDF

Elucidation of rutin's role in inducing caspase-dependent apoptosis via HPV-E6 and E7 down-regulation in cervical cancer HeLa cells.

Biosci Rep 2021 Jun;41(6)

Department of Zoology, University of Lucknow, Lucknow 226007, India.

Over the recent few years rutin has gained wider attention in exhibiting inhibitory potential against several oncotargets for inducing apoptotic and antiproliferative activity in several human cancer cells. Several deregulated signaling pathways are implicated in cancer pathogenesis. Therefore we have inclined our research towards exploring the anticancerous efficacy of a very potent phytocompound for modulating the incontinent expression of these two crucial E6 and E7 oncogenes. Read More

View Article and Full-Text PDF

Pseudo-ginsenoside Rh2 Induces Protective Autophagy in Hepatocellular Carcinoma HepG2 Cells.

Recent Pat Anticancer Drug Discov 2021 Jun 6. Epub 2021 Jun 6.

Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun, 130021, Jilin Province, China.

Background: Pseudo-ginsenoside-Rh2 (pseudo-G-Rh2), a novel derivative of ginsenoside Rh2, is reported to exert a pro-apoptotic effect on various malignancies. However, whether this anti-cancer action of pseudo-G-Rh2 involves autophagy remains to be determined and explored.

Objectives: Investigation of pseudo-G-Rh2-induced apoptosis and autophagy and the underlying mechanism. Read More

View Article and Full-Text PDF

[The Inhibiting Effect of Autophagy Inhibitor ROC-325 on Multiple Myeloma].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Jun;29(3):797-804

Institute of Hematology, Xuzhou Medical University; Department of Hematology, Xuzhou Medical University Affiliated Hospital, Xuzhou 221002, Jiangsu Province, China,E-mail:

Objective: To investigate the effects of autophagy inhibitor ROC-325 and its combination with bortezomib on the proliferation, apoptosis and autophagy of multiple myeloma cell lines.

Methods: Multiple myeloma cells were treated with ROC-325 at different concentration. The cell proliferation was detected by CCK-8. Read More

View Article and Full-Text PDF

LncRNA TP73-AS1 promotes oxidized low-density lipoprotein-induced apoptosis of endothelial cells in atherosclerosis by targeting the miR-654-3p/AKT3 axis.

Cell Mol Biol Lett 2021 Jun 8;26(1):27. Epub 2021 Jun 8.

Stomatological Hospital, Southern Medical University, No. 366 Jiangnan Avenue South, Haizhu District, Guangzhou City, Guangdong Province, People's Republic of China.

Background: Although lncRNA TP73-AS1 has been shown to play important roles in various human diseases, its function in atherosclerosis (AS) remains unclear.

Methods: Human aortic endothelial cells (HAECs) were treated with 50 μg/ml oxidized low-density lipoprotein (ox-LDL) to establish an atherosclerotic cell model. The expression of TP73-AS1, miR-654-3p and AKT3 was detected by qRT-PCR. Read More

View Article and Full-Text PDF

Incaspitolide A extracted from Carpesium cernuum induces apoptosis in vitro via the PI3K/AKT pathway in benign prostatic hyperplasia.

Biosci Rep 2021 Jun;41(6)

Department of Biomedicine, Guizhou University, Guiyang 550002, P.R. China.

Benign prostatic hyperplasia (BPH) is a common disease that occurs mainly in older men. The pathogenesis of BPH is complex and patients face a prolonged treatment course, and novel drugs with better selectivity and lower toxicity are required. Incaspitolide A (compound TMJ-12) is a germacrane-type sesquiterpenoid compound extracted from the plant Carpesium carnuum. Read More

View Article and Full-Text PDF

Acute cadmium exposure induces GSDME-mediated pyroptosis in triple-negative breast cancer cells through ROS generation and NLRP3 inflammasome pathway activation.

Environ Toxicol Pharmacol 2021 Jun 5;87:103686. Epub 2021 Jun 5.

Department of Pathology, and the Key-Innovative Discipline of Molecular Diagnostics, Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing University, Jiaxing, 314001, ZJ, China. Electronic address:

Cadmium (Cd) exposure can exert an impact on carcinogenicity of breast cancer, however, the mechanism is not fully understood in triple-negative breast cancer (TNBC). We performed a TNBC MDA-MB-231 cell model and assessed the toxic effect of Cd exposure (0, 10, 20, 50, 60, 80 μM). Cd reduced cell viability in a time- and dose-dependent manner, followed by cell cycle arrest in S phase with alterations of cyclin 1A1, cyclin 1D1 and CDK2. Read More

View Article and Full-Text PDF

Thiamine Alleviates High-Concentrate-Diet-Induced Oxidative Stress, Apoptosis, and Protects the Rumen Epithelial Barrier Function in Goats.

Front Vet Sci 2021 20;8:663698. Epub 2021 May 20.

Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.

High-concentrate diets are continually used in ruminants to meet the needs of milk yield, which can lead to the occurrence of subacute rumen acidosis in ruminants. This study investigated the protective effects of dietary thiamine supplementation on the damage of the ruminal epithelium barrier function in goats fed a high-concentrate diet. Twenty-four healthy Boer goats (live weight of 35. Read More

View Article and Full-Text PDF

Role of lincRNA-Cox2 targeting miR-150 in regulating the viability of chondrocytes in osteoarthritis.

Exp Ther Med 2021 Aug 25;22(2):800. Epub 2021 May 25.

Department of Anesthesiology, Qingdao No. 6 People's Hospital, Qingdao, Shandong 266033, P.R. China.

Osteoarthritis (OA) is a joint disease characterised by progressive cartilage degradation and inflammation, but the detailed pathogenesis of OA remains unclear. The present study aimed to investigate the role of long intergenic non-coding RNA (lincRNA)-Cox2 in OA progression and the potential mechanism. An OA mouse model was used for experiments, and IL-1β-induced injury of mouse chondrocytes was conducted for experiments. Read More

View Article and Full-Text PDF