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Small footprint liquid chromatography-mass spectrometry for pharmaceutical reaction monitoring and automated process analysis.

J Chromatogr A 2021 Sep 10;1656:462545. Epub 2021 Sep 10.

ARC Training Centre for Portable Analytical Separation Technologies (ASTech), School of Natural Sciences, University of Tasmania, Private Bag 75, Hobart, Tasmania 7001, Australia; Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences, University of Tasmania, Private Bag 75, Hobart, Tasmania 7001, Australia. Electronic address:

Liquid chromatography (LC) has broad applicability in the pharmaceutical industry, from the early stages of drug discovery to reaction monitoring and process control. However, small footprint, truly portable LC systems have not yet been demonstrated and fully evaluated practically for on-line, in-line or at-line pharmaceutical analysis. Herein, a portable, briefcase-sized capillary LC fitted with a miniature multi-deep UV-LED detector has been developed and interfaced with a portable mass spectrometer for on-site pharmaceutical analysis. Read More

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September 2021

Single Genome Sequencing of Expressed and Proviral HIV-1 Envelope Glycoprotein 120 () and Genes.

Bio Protoc 2017 Jun 20;7(12):e2334. Epub 2017 Jun 20.

Departments of Laboratory Medicine, Pathology, and Medicine, University of California at San Francisco, San Francisco, California, USA.

The current study provides detailed protocols utilized to amplify the complete HIV-1 and genes from single copies of expressed or integrated HIV present in fresh-frozen autopsy tissues of patients who died while on combined antiretroviral therapy (cART) with no detectable plasma viral load (pVL) at death ( Lamers , 2016a and 2016b; Rose , 2016 ). This method optimizes protocols from previous publications ( Palmer , 2005 ; Norström , 2012 ; Lamers , 2015 ; 2016a and 2016b; Rife , 2016 ) to produce single distinct PCR products that can be directly sequenced and includes several cost-saving and time-efficient modifications. Read More

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Impact of the COVID-19 Pandemic on Higher Education: Characterizing the Psychosocial Context of the Positive and Negative Affective States Using Classification and Regression Trees.

Front Psychol 2021 1;12:714397. Epub 2021 Sep 1.

Department of Clinical Psychology and Psychobiology, Universitat de Barcelona, Barcelona, Spain.

Our aim is to analyze the extent to which the psychosocial aspects can characterize the affective states of the teachers, administrative staff, and undergraduate and postgraduate students during the quarantine. A questionnaire was answered by 1,328 people from the community of the (UB), Spain. The survey was partially designed , collecting indicators related to sociodemographic variables, the impact of COVID on the subjects or in their personal context, the psychosocial context of coexistence and perceived social support, characteristics related to the physical context during the quarantine, and labor conditions. Read More

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September 2021

Sterility Assurance-Current & Future State.

PDA J Pharm Sci Technol 2021 Sep 16. Epub 2021 Sep 16.

United States Pharmacopiea.

Manufacture by aseptic processing accompanied by end product compendial sterility testing has been the predominant means of production and disposition of therapeutics requiring the critical quality attribute of sterility. Despite significant advancements in microbiology, epidemiology, innovations in therapeutic products, and engineering there have been minimal advancement in the standards and regulations governing the assurance of sterility. Furthermore, the assurance of sterility of current and future therapies are not well served in a single fashion rather that therapies occupy optimal locations in a sterility assurance design space within which parametric release is the default and expected mode of product disposition. Read More

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September 2021

Neurological manifestations in people living with HIV/AIDS in the late cART era: a prospective observational study at a tertiary healthcare center in São Paulo, Brazil.

HIV Res Clin Pract 2021 Sep 16:1-9. Epub 2021 Sep 16.

Departamento de Infectologia, Instituto de Infectologia Emílio Ribas, São Paulo, SP, Brazil.

The aim of this study was to evaluate the frequency, spectrum, in-hospital mortality rate, and factors associated with death in people living with HIV/AIDS (PLWHA) presenting with neurological diseases from a middle-income country, as well as estimate its one-year global death rate.This prospective observational cohort study was conducted at a Brazilian tertiary health center between January and July 2017. HIV-infected patients above 18 years of age who were admitted due to neurological complaints were consecutively included. Read More

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September 2021

Genotypic HIV-1 tropism determination might help to identify people with exhausted treatment options and advanced disease.

J Antimicrob Chemother 2021 Sep 16. Epub 2021 Sep 16.

Department of Experimental Medicine, University of Rome 'Tor Vergata', Rome, Italy.

Objectives: To evaluate HIV-1 tropism in 1382 combined antiretroviral therapy (cART)-experienced patients failing therapy to characterize those with exhausted therapeutic options.

Methods: HIV-1 genotypic tropism was inferred through Geno2Pheno by estimating the false-positive-rate (FPR) values. Cumulative resistance and drug activity were evaluated by Stanford algorithm. Read More

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September 2021

Comparison of the Performance of Decision Tree (DT) Algorithms and ELM model in the Prediction of Water Quality of the Upper Green River watershed.

Water Environ Res 2021 Sep 15. Epub 2021 Sep 15.

Department of Civil Engineering, Birla Institute of Technology and Science, Pilani, Hyderabad Campus, Jawahar Nagar, Kapra (Mandal), Medchal District, Hyderabad, Telangana, India.

Stream waters play a crucial role in catering to the world's needs with the required quality of water. Due to the discharges of wastewater from the various point and nonpoint sources, most of the watersheds are contaminated easily. The Upper Green River watershed in Kentucky, USA, is one such watershed which is contaminated over the years due to the runoff from rural areas and agricultural lands and combined sewer overflows (CSOs) from urban areas. Read More

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September 2021

The Safety of Bridging Radiation with Anti-BCMA CAR T-Cell Therapy for Multiple Myeloma.

Clin Cancer Res 2021 Sep 15. Epub 2021 Sep 15.

Radiation Oncology, University of Pennsylvania.

Purpose: B-cell maturation antigen (BCMA)-targeted chimeric antigen receptor (CAR) T-cells (CART-BCMA) are a promising treatment for relapsed/refractory multiple myeloma (r/rMM). We evaluated the safety and feasibility of bridging radiation (RT) in subjects treated on a phase 1 trial of CART-BCMA.

Experimental Design: Twenty-five r/rMM subjects were treated in 3 cohorts with two doses of CART-BCMA cells +/- cyclophosphamide. Read More

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September 2021

Leveraging Google Earth Engine platform to characterize and map small seasonal wetlands in the semi-arid environments of South Africa.

Sci Total Environ 2021 Sep 4;803:150139. Epub 2021 Sep 4.

Institute for Water Studies, Department of Earth Science, University of the Western Cape, Private Bag X17, Bellville, 7535 Cape Town, South Africa.

Although significant scientific research strides have been made in mapping the spatial extents and ecohydrological dynamics of wetlands in semi-arid environments, the focus on small wetlands remains a challenge. This is due to the sensing characteristics of remote sensing platforms and lack of robust data processing techniques. Advancements in data analytic tools, such as the introduction of Google Earth Engine (GEE) platform provides unique opportunities for improved assessment of small and scattered wetlands. Read More

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September 2021

Legacy effect on neuropsychological function in HIV-infected men on combination antiretroviral therapy.

AIDS 2021 Sep 13. Epub 2021 Sep 13.

Departments of Statistics, University of Pittsburgh Departments of Psychiatry, University of Pittsburgh Departments of Biostatistics, University of Pittsburgh Departments of Epidemiology, Graduate School of Public Health, University of Pittsburgh Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh Department of Neurology, David Geffen School of Medicine, UCLA Department of Psychiatry, Rush University School of Medicine Departments of Psychiatry, The Johns Hopkins University School of Medicine Department of Radiology, Northwestern University Neurology, The Johns Hopkins University School of Medicine Department of Epidemiology, Bloomberg School of Public Health, The Johns Hopkins University Departments of Neurology, University of Pittsburgh Departments of Psychology, University of Pittsburgh.

Objective: To determine whether cART initiation alters the trajectory of cognitive performance in HIV+ men, and whether cognition prior to cART predicts post-cART function.

Design: Longitudinal cohort study. Multicenter AIDS Cohort Study. Read More

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September 2021

Stratifying the risk of NAFLD in patients with HIV under combination antiretroviral therapy (cART).

EClinicalMedicine 2021 Oct 5;40:101116. Epub 2021 Sep 5.

Department of Internal Medicine I, University Hospital Frankfurt, Germany.

Background: De novo steatosis is the main criteria for non-alcoholic fatty liver disease (NAFLD), which is becoming a clinically relevant comorbidity in HIV-infected patients. This may be due to the HIV virus itself, as well as long-term toxicities deriving from antiretroviral therapy. Therefore, HIV infected patients require prevention and monitoring regarding NAFLD. Read More

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October 2021

MR spectroscopy and diffusion imaging in people with human immunodeficiency virus: Relationships to clinical and immunologic findings.

J Neuroimaging 2021 Sep 14. Epub 2021 Sep 14.

Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DiNOGMI), University of Genoa, Genoa, Italy.

Background And Purpose: People with human immunodeficiency virus (HIV; PWH) present a complex array of immunologic and medical disorders that impact brain structure and metabolism, complicating the interpretation of neuroimaging. This pilot study of well-characterized multi-morbid PWH examined how medical and immunologic factors predicted brain characteristics on proton MR spectroscopy (1H-MRS) and diffusion-weighted imaging (DWI).

Methods: Eighteen individuals on combination antiretroviral therapy (cART), with mean age of 56 years, underwent medical history review, neuroimaging, and on the day of imaging, blood draw for assay of 20 plasma cytokines and flow cytometric characterization of peripheral blood mononuclear cell subsets. Read More

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September 2021

Chimeric antigen receptor T cells self-neutralizing IL6 storm in patients with hematologic malignancy.

Cell Discov 2021 Sep 14;7(1):84. Epub 2021 Sep 14.

The First Affiliated Hospital of University of Science and Technology of China (USTC), Hefei, Anhui, China.

IL6 is one of the most elevated cytokines during chimeric antigen receptor (CAR) T cell cytokine release syndrome (CRS), and IL6R blockade by Tocilizumab has successfully relieved the most life-threatening aspects of CRS in patients. In addition, latest studies demonstrated the essential role of IL1 in driving CART induced neurotoxicity in mouse models. Here we present a clinical investigation (ChiCTR2000032124; ChiCTR2000031868) of anti-CD19 and anti-BCMA CART (41BBζ) secreting an anti-IL6 scFv and IL1 receptor antagonist (IL1RA) in treating patients with hematologic malignancy. Read More

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September 2021

Overfeeding during development induces temporally-dependent changes in areas controlling food intake in the brains of male Wistar rats.

Life Sci 2021 Sep 11;285:119951. Epub 2021 Sep 11.

Neuropsychiatry and Behavior Science Graduate Program, Universidade Federal de Pernambuco -UFPE-Recife, PE, Brazil; Laboratory of Biochemistry and Exercise Biochemistry, Universidade Federal de Pernambuco - UFPE, Recife, PE, Brazil. Electronic address:

Aims: We sought to evaluate the effects of overfeeding during lactation on the feeding behavior and expression of specific regulatory genes in brain areas associated with food intake in 22- and 60-day old male rats.

Methods: We evaluated body weight, food intake of standard and palatable diet, and mRNA expression of dopamine receptor D1 (DDR1), dopamine receptor (DDR2), melanocortin 4 receptor (MC4R), the μ-opioid receptor (MOR), neuropeptide Y (NPY), agouti-related protein (AGRP), proopiomelanocortin (POMC), cocaine-and amphetamine-regulated transcript (CART), serotonin (5-hydroxytryptamine; 5-HT) transporter (SERT), 5-hydroxytryptamine receptor 1B (5-HT1B), 5-hydroxytryptamine receptor 2C receptor (5-HT2C), Clock (CLOK), cryptochrome protein 1 (Cry1) and period circadian protein homolog 2 (Per2) in the striatum, hypothalamus and brainstem of male rats at post-natal days (PND) 22 and 60.

Key Findings: Overfeeding resulted in significantly increased body weight through PND60, and a 2-fold increase in palatable food intake at PND22, but not at PND60. Read More

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September 2021

A Default Option to Improve Nutrition for Adults With Low Income Using a Prefilled Online Grocery Shopping Cart.

J Nutr Educ Behav 2021 Sep;53(9):759-769

Department of Psychology, University at Albany, State University of New York, Albany, NY.

Objective: To determine whether a prefilled online grocery shopping (default) cart improves the nutritional quality of groceries purchased compared with receiving nutrition education (NE).

Design: Longitudinal study.

Setting: Three food pantries in the US. Read More

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September 2021

The impact of the 2014 Ebola epidemic on HIV disease burden and outcomes in Liberia West Africa.

PLoS One 2021 10;16(9):e0257049. Epub 2021 Sep 10.

Division of Biostatistics, School of Public Health, University of Minnesota, Minneapolis, Minnesota, United States of America.

Background: Detailed longitudinal studies of HIV-positive individuals in West Africa are lacking. Here the HIV prevalence, incidence, all-cause mortality, and the proportion of individuals receiving treatment with cART in two cohorts of participants in Ebola-related studies are described.

Setting: Individuals of all ages were enrolled and followed at four sites in the area of Monrovia, Liberia. Read More

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September 2021

3- or 1-Month DAPT in Patients at High Bleeding Risk Undergoing Everolimus-Eluting Stent Implantation.

JACC Cardiovasc Interv 2021 Sep;14(17):1870-1883

Cardiocentro Ticino, Lugano and Bern University Hospital, Bern, Switzerland.

Objectives: The aim of this study was to evaluate 2 abbreviated dual-antiplatelet therapy (DAPT) regimens in patients at high bleeding risk (HBR) undergoing percutaneous coronary intervention (PCI).

Background: Current-generation drug-eluting stents are preferred over bare-metal stents for HBR patients, but their optimal DAPT management remains unknown.

Methods: The XIENCE Short DAPT program included 3 prospective, multicenter, single-arm studies enrolling HBR patients who underwent successful PCI with a cobalt-chromium everolimus-eluting stent. Read More

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September 2021

Anti-HIV aptamers Challenges and Prospects.

Curr HIV Res 2021 Sep 7. Epub 2021 Sep 7.

Department of Virology, National Health Laboratory Service/University of KwaZulu-Natal, c/o Inkosi Albert Luthuli Central Hospital, 5th Floor Laboratory Building, 800 Bellair Road, Mayville, Durban 4091. South Africa.

Human immunodeficiency virus (HIV) infection continues to be a significant health burden in many countries around the world. Current HIV treatment through a combination of different antiretroviral drugs (cART) effectively suppresses viral replication, but drug resistance and cross-resistance are significant challenges. This has prompted the search for novel targets and agents, such as nucleic acid aptamers. Read More

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September 2021

Hodgkin Lymphoma in People Living with HIV.

Cancers (Basel) 2021 Aug 29;13(17). Epub 2021 Aug 29.

Department of Hematology, Institute Català d'Oncologia-Germans Trias i Pujol Hospital, Carretera de Canyet s/n, 08916 Barcelona, Spain.

Despite widespread use of combined antiretroviral therapy (cART) and increased life expectancy in people living with HIV (PLWH), HIV-related lymphomas (HRL) remain a leading cause of cancer morbidity and mortality for PLWH, even in patients optimally treated with cART. While the incidence of aggressive forms of non-Hodgkin lymphoma decreased after the advent of cART, incidence of Hodgkin lymphoma (HL) has increased among PLWH in recent decades. The coinfection of Epstein-Barr virus plays a crucial role in the pathogenesis of HL in the HIV setting. Read More

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Nocturnal Oxygen Saturation Parameters as Independent Risk Factors for Type 2 Diabetes Mellitus among Obstructive Sleep Apnea Patients.

J Clin Med 2021 Aug 24;10(17). Epub 2021 Aug 24.

Department of Sleep Medicine and Metabolic Disorders, Medical University of Lodz, 92-215 Lodz, Poland.

Obstructive sleep apnea (OSA) is a recognized independent risk factor for metabolic disorders, type 2 diabetes mellites (DM2) in particular. Therefore, the study aimed to assess the influence of nocturnal oxygen saturation parameters on the onset of DM2 among OSA patients. The study consisted of 549 participants, who underwent polysomnography examination. Read More

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Visual and somatosensory feedback mechanisms of precision manual motor control in autism spectrum disorder.

J Neurodev Disord 2021 09 8;13(1):32. Epub 2021 Sep 8.

Life Span Institute, University of Kansas, Lawrence, KS, USA.

Background: Individuals with autism spectrum disorder (ASD) show deficits processing sensory feedback to reactively adjust ongoing motor behaviors. Atypical reliance on visual and somatosensory feedback each have been reported during motor behaviors in ASD suggesting that impairments are not specific to one sensory domain but may instead reflect a deficit in multisensory processing, resulting in reliance on unimodal feedback. The present study tested this hypothesis by examining motor behavior across different visual and somatosensory feedback conditions during a visually guided precision grip force test. Read More

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September 2021

Extracellular vesicles derived from CAR-T Cells: A potential therapy for cancer.

Hum Gene Ther 2021 Sep 8. Epub 2021 Sep 8.

Tel Aviv Sourasky Medical Center, 26738, 6th Weizmann st, Tel Aviv, Tel Aviv, Israel, 64239;

Chimeric antigen receptor (CAR)-T cells are genetically engineered T cells, directed against a tumor associated antigen (TAA). Extracellular-vesicles (EVs) derived from CAR-T cells (CAR-T EVs) may preserve CAR-T activity and overcome one of the major obstacles responsible for CAR-T cell failure in patients with solid tumors. The study aimed to compare CAR-T EVs to their parental cells and explore their cell penetration and cytotoxic activity. Read More

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September 2021

Role of environmental surfaces and hands of healthcare workers in perpetuating multi-drug-resistant pathogens in a neonatal intensive care unit.

Eur J Pediatr 2021 Sep 6. Epub 2021 Sep 6.

Department of Microbiology, High Institute of Public Health, Alexandria University, El-Ibrahimia, 165 El-Horreya Avenue, Alexandria, Egypt.

Neonates admitted to neonatal intensive care units are at a risk of developing healthcare-associated infections, leading to increased risk of mortality. This study aimed to identify organisms causing such late-onset infections in neonates and determine whether these isolates were genetically identical to those from the surrounding environmental surfaces and hands of healthcare workers (HCWs). A cross-sectional study was carried out over a period of 4 months in a university neonatal intensive care unit (NICU). Read More

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September 2021

The Ciji-Hua'ai-Baosheng II Formula Attenuates Chemotherapy-Induced Anorexia in Mice With H Hepatocellular Carcinoma.

Front Pharmacol 2021 19;12:715824. Epub 2021 Aug 19.

Department of Traditional Chinese Medicine, School of Medicine, Xiamen University, Xiamen, China.

Ciji-Hua'ai-Baosheng II Formula (CHB-II-F) is a traditional Chinese medicine formula, which specifically targets different aspects of chemotherapy-induced adverse effects in patients with cancer. In our clinical application, CHB-II-F significantly alleviated chemotherapy-induced anorexia (loss of appetite) and improved the quality of life for patients with tumor during and after chemotherapy. However, the mechanism of CHB-II-F in alleviation of chemotherapy-induced anorexia remains to be further investigated. Read More

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Development of a clinical prediction rule for patients with cervical spinal cord injury who have difficulty in obtaining independent living.

Spine J 2021 Sep 3. Epub 2021 Sep 3.

Graduate School of Humanities and Social Sciences, Hiroshima University, Hiroshima, Japan.

Background Context: A simple and easy to use clinical prediction rule (CPR) to detect patients with a cervical spinal cord injury (SCI) who would have difficulty in obtaining independent living status is vital for providing the optimal rehabilitation and education in both care recipients and caregivers. A machine learning approach was recently applied to the field of rehabilitation and has the possibility to develop an accurate and useful CPR.

Purpose: The aim of this study was to develop and assess a CPR using a decision tree algorithm for predicting which patients with a cervical SCI would have difficulty in obtaining an independent living. Read More

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September 2021

Leptin Chronic Effect on Differentiation, Ion Currents and Protein Expression in N1E-115 Neuroblastoma Cells.

Pak J Biol Sci 2021 Jan;24(3):297-309

<b>Background and Objective:</b> Arcuate nucleus (ARC), a component of appetite-regulatory factors, contains populations of both orexigenic and anorexigenic neurons and one of the fundamental components of its system is leptin. Studies have evidenced the critical neurotrophic role in the development of ARC. To determine such effects on neuron development, N1E-115 neuroblastoma cells were used as an ARC model. Read More

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January 2021

Polymyalgia rheumatica: A case series from Colombia and analysis of Latin America.

J Transl Autoimmun 2021 21;4:100115. Epub 2021 Aug 21.

Center for Autoimmune Diseases Research (CREA), School of Medicine and Health Sciences, Universidad del Rosario, Bogota, Colombia.

Objective: Polymyalgia rheumatica (PMR) is the most common inflammatory disease in patients over 50 years. Information about the disease in Latin America (LATAM) is scarce. We aimed to evaluate a group of Colombian patients with PMR and to conduct a systematic review of PMR in LATAM. Read More

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Eco-environmental assessment model of the mining area in Gongyi, China.

Sci Rep 2021 Sep 2;11(1):17549. Epub 2021 Sep 2.

Institute of Geophysics and Geomatics, China University of Geosciences, Wuhan, 430074, China.

The ecological environment directly affects human life. One of the ecological environmental issues that China is presently facing is deterioration of the ecological environment due to mining. The pollution produced by mining causes the destruction of land, water bodies, the atmosphere, and vegetation resources and new geological problems that seriously impact human civilization and life. Read More

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September 2021

Incidence and associated factors of type 2 diabetes mellitus onset in the Brazilian HIV/AIDS cohort study.

Braz J Infect Dis 2021 Aug 31;25(4):101608. Epub 2021 Aug 31.

Department and Division of Infectious and Parasitic Diseases, Hospital das Clinicas, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil. Electronic address:

Background: People living with HIV (PLH) under combined antiretroviral therapy (cART) are at risk of developing type 2 diabetes mellitus (T2DM).

Objective: We examined the incidence of T2DM, associated factors and mean time to outcome in PLH under cART.

Method: Data for this multicenter cohort study were obtained from PLH aged over 18, who started cART in 13 Brazilian sites from 2003 to 2013. Read More

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Hematological cancers in individuals infected by HIV.

Blood 2021 Sep 1. Epub 2021 Sep 1.

Fondazione IRCCS, Istituto Nazionale Tumori, Milano, Italy.

HIV infection increases cancer risk and is linked to cancers associated to infectious agents classified as carcinogenic to humans by the International Agency for Research on Cancer. Lymphomas represent one of the most frequent malignancies among individuals infected by HIV. Diffuse large B-cell lymphoma remains a leading cancer after the introduction of combined antiretroviral therapy (cART). Read More

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September 2021