26,171 results match your criteria cardiac differentiation

Mechanism of Baihe Decoction in the treatment of coronary heart disease based on network pharmacology and molecular docking.

Ann Palliat Med 2021 Mar;10(3):3205-3218

Henan University of Chinese Medicine, Zhengzhou, China.

Background: Network pharmacology is widely used in mechanistic studies of traditional Chinese medicines (TCMs). The present study aimed to predict the target and signaling pathway of Baihe Decoction in the intervention of coronary heart disease (CHD) based on a network pharmacology approach and molecular docking.

Methods: The active ingredients of Baihe Decoction were screened by the Traditional Chinese Medicine Systems Pharmacology (TCMSP), and their potential target genes and proteins in CHD were predicted. Read More

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Defining Optimal Enzyme and Matrix Combination for Replating of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes at Different Levels of Maturity.

Exp Cell Res 2021 Apr 10:112599. Epub 2021 Apr 10.

Regenerative and Restorative Medicine Research Center (REMER), Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, Istanbul, Turkey; Department of Medical Biology, School of Medicine, Istanbul Medipol University, Istanbul, Turkey; Medical Biology and Genetics Graduate Program, Health Sciences Institute, Istanbul Medipol University, Istanbul, Turkey. Electronic address:

Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) create an unlimited cell source for basic and translational research. Depending on the maturity of cardiac cultures and the intended downstream application, obtaining hiPSC-CMs as a single-cell, monolayer or three-dimensional clusters can be challenging. Here, we defined strategies to replate hiPSC-CMs on early days (D15-30) or later more mature (D60-150) differentiation cultures. Read More

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Donor cell memory confers a metastable state of directly converted cells.

Cell Stem Cell 2021 Apr 7. Epub 2021 Apr 7.

Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster 48149, Germany; Faculty of Medicine, University of Münster, Münster 48149, Germany. Electronic address:

Generation of induced oligodendrocyte progenitor cells (iOPCs) from somatic fibroblasts is a strategy for cell-based therapy of myelin diseases. However, iOPC generation is inefficient, and the resulting iOPCs exhibit limited expansion and differentiation competence. Here we overcome these limitations by transducing an optimized transcription factor combination into a permissive donor phenotype, the pericyte. Read More

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Geometric parameters on MRA source images to differentiate small Proximal Posterior communicating artery aneurysms from Infundibular dilation.

J Neuroimaging 2021 Apr 13. Epub 2021 Apr 13.

Department of Radiology, Kangdong Seong-Sim Hospital, Hallym University College of Medicine, Seoul, South Korea.

Background And Purpose: We aimed to assess the accuracy of magnetic resonance angiography (MRA) in the differentiation of small aneurysms versus infundibular dilations (IDs) at the internal carotid artery-posterior communicating artery (ICA-PComA) junction, emphasizing the role of MRA axial source images.

Methods: This retrospective study consisted of 83 focal arterial protrusions at ICA-PComA junction in 76 patients who underwent both MRA and digital subtraction angiography (DSA)/3-dimensional rotational angiography (3DRA). The diagnostic performance of MRA for differential diagnosis of aneurysm from ID was calculated using DSA/3DRA interpretation as the standard of reference. Read More

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Colchicine acts selectively in the liver to induce hepatokines that inhibit myeloid cell activation.

Nat Metab 2021 Apr 12. Epub 2021 Apr 12.

Department of Systems Biology, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.

Colchicine has served as a traditional medicine for millennia and remains widely used to treat inflammatory and other disorders. Colchicine binds tubulin and depolymerizes microtubules, but it remains unclear how this mechanism blocks myeloid cell recruitment to inflamed tissues. Here we show that colchicine inhibits myeloid cell activation via an indirect mechanism involving the release of hepatokines. Read More

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Homeodomain only protein X (HOPX) has an oncogenic activity during squamous skin carcinogenesis.

J Invest Dermatol 2021 Apr 9. Epub 2021 Apr 9.

Department of Dermatology and Venereology, University Hospital Centre (CHUV) and University of Lausanne (UNIL), Lausanne, Switzerland. Electronic address:

Cutaneous squamous cell carcinomas (cSCC) are frequent heterogeneous tumours, arising from sun-exposed regions of the skin and characterized by complex pathogenesis. HOPX is a member of the homeodomain-containing superfamily of proteins, holding an atypical homeodomain unable to bind DNA. First discovered in the heart as a regulator of cardiac development, in skin HOPX modulates terminal differentiation of keratinocytes. Read More

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Medical Education and Training Within Congenital Cardiology: Current Global Status and Future Directions in A Post COVID-19 World.

Cardiol Young 2021 Apr 12:1-40. Epub 2021 Apr 12.

Department of Cardiology, Mazankowski Heart Institute, University of Alberta, Edmonton, Alberta, Canada.

Despite enormous strides in our field with respect to patient care, there has been surprisingly limited dialogue on how to train and educate the next generation of congenital cardiologists. This paper reviews the current status of training and evolving developments in medical education pertinent to congenital cardiology. The adoption of competency-based medical education has been lauded as a robust framework for contemporary medical education over the last two decades. Read More

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Rational, unbiased selection of reference genes for pluripotent stem cell-derived cardiomyocytes.

Tissue Eng Part C Methods 2021 Apr 11. Epub 2021 Apr 11.

University of Wisconsin-Madison, Chemical and Biological Engineering, 1415 Engineering Drive, Madison, Wisconsin, United States, 53593;

RT-qPCR is a powerful technique to quantify gene expression via transcript abundance. Expression of target genes is normalized to expression of stable reference genes to account for sample preparation variability. Thus, the identification and validation of stably-expressed reference genes is crucial to making accurate, quantitative, statistical conclusions in gene expression studies. Read More

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Biologically derived, 3-dimensional, embryonic scaffolds for long-term cardiomyocyte culture.

Stem Cells Dev 2021 Apr 11. Epub 2021 Apr 11.

University of Minnesota, Internal Medicine/Cardiology, Minneapolis, Minnesota, United States;

The ability to maintain viable cultures of mature, primary cardiomyocytes is challenging. The lack of viable cardiomyocyte cultures severely limits in vitro biochemical assays, toxicology assays, drug screening assays and other analyses. Here, we describe a novel 3-dimensional (3D) embryonic scaffold, which supports the culture of postnatal day 7 (P7) murine cardiomyocytes within the embryonic heart for, at least, 28 days. Read More

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Sudden Cardiac Death and Sudden Cardiac Arrest in Patients with Human Immunodeficiency Virus: A Systematic Review.

Cureus 2021 Mar 8;13(3):e13764. Epub 2021 Mar 8.

Internal Medicine, McLaren Flint, Flint, USA.

The importance of this review lies in its study of the risk of sudden cardiac death (SCD) and sudden cardiac arrest (SCA) in people living with the human immunodeficiency virus (PLWH). To the best of our knowledge, this is the first review investigating the effect of the human immunodeficiency virus (HIV) on SCD and SCA. The review's objective was to determine the risk of SCD and SCA in PLWH. Read More

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Unstable angina complicated with dynamic left ventricular outflow tract obstruction.

J Cardiol Cases 2021 Apr 12;23(4):181-188. Epub 2021 Feb 12.

Department of Cardiovascular Biology and Medicine, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachidori, Chuo-ku, Niigata 951-8510, Japan.

Left ventricular outflow tract obstruction (LVOTO) complicated with unstable angina (uAP) has not been described widely, but patients with these two conditions have several problems. Differentiation of the two conditions is also often difficult because the chest symptoms are similar. Moreover, nitrates are commonly used for ischemic heart disease, but have the effect of worsening LVOTO. Read More

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Direct and Indirect Somatic Embryogenesis Induction in Abel.

Front Plant Sci 2021 26;12:644389. Epub 2021 Mar 26.

Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, The College of Forestry, Beijing Forestry University, Beijing, China.

Abel. is an important woody oil species; however, the shortage of rapid and industrialized seedling culture is a large constraint on the development of the tea oil industry. Somatic embryogenesis (SE) is one of the main powerful biotechnological tools for plant mass regeneration, but the largely unknown SE in limits the scale production of clonal plants. Read More

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Non-coding RNAs in Cardiac Regeneration.

Front Physiol 2021 24;12:650566. Epub 2021 Mar 24.

Institute of Cardiovascular Regeneration, Center for Molecular Medicine, Goethe University Frankfurt, Frankfurt am Main, Germany.

The adult heart has a limited capacity to replace or regenerate damaged cardiac tissue following severe myocardial injury. Thus, therapies facilitating the induction of cardiac regeneration holds great promise for the treatment of end-stage heart failure, and for pathologies invoking severe cardiac dysfunction as a result of cardiomyocyte death. Recently, a number of studies have demonstrated that cardiac regeneration can be achieved through modulation and/or reprogramming of cardiomyocyte proliferation, differentiation, and survival signaling. Read More

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Aryl hydrocarbon receptor is essential for the pathogenesis of pulmonary arterial hypertension.

Proc Natl Acad Sci U S A 2021 Mar;118(11)

Department of Vascular Physiology, National Cerebral and Cardiovascular Center Research Institute, 564-8565 Suita, Japan;

Pulmonary arterial hypertension (PAH) is a devastating disease characterized by arteriopathy in the small to medium-sized distal pulmonary arteries, often accompanied by infiltration of inflammatory cells. Aryl hydrocarbon receptor (AHR), a nuclear receptor/transcription factor, detoxifies xenobiotics and regulates the differentiation and function of various immune cells. However, the role of AHR in the pathogenesis of PAH is largely unknown. Read More

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Genetic deletion of Nox4 enhances cancerogen-induced formation of solid tumors.

Proc Natl Acad Sci U S A 2021 Mar;118(11)

Institute for Cardiovascular Physiology, Goethe University, 60590 Frankfurt am Main, Germany;

Reactive oxygen species (ROS) can cause cellular damage and promote cancer development. Besides such harmful consequences of overproduction of ROS, all cells utilize ROS for signaling purposes and stabilization of cell homeostasis. In particular, the latter is supported by the NADPH oxidase 4 (Nox4) that constitutively produces low amounts of HO By that mechanism, Nox4 forces differentiation of cells and prevents inflammation. Read More

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