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Performance of cervical cancer screening and triage strategies among women living with HIV in China.

Cancer Med 2021 Aug 2. Epub 2021 Aug 2.

Department of Cancer Epidemiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Objectives: To evaluate the clinical performance of liquid-based cytology (LBC), HPV tests and visual inspections with acetic acid or Lugol's iodine (VIA/VILI) as primary screening and triage strategies among Chinese women living with HIV (WLHIV).

Methods: WLHIV aged 18 years and older were recruited from HIV/AIDS treatment clinic in Yunnan, China from 2019 to 2020. Women were screened with self- and physician-sampling for HPV tests, LBC, and VIA/VILI. Read More

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Validation of a Chin Retrusion Scale for Chinese Subjects.

J Craniofac Surg 2021 Jul 13. Epub 2021 Jul 13.

Peking University International Hospital Tsinghua University Affiliated Beijing Tsinghua Changgung Hospital Beijing Hospital Allergan Aesthetics, an AbbVie Company, Beijing, China Allergan Aesthetics, an AbbVie Company, Irvine, CA Beijing University Third Hospital, Beijing Shi, China.

Abstract: Chin augmentation procedures are gaining in popularity. The purpose of this study was to validate the China (Allergan) Chin Retrusion Scale (CACRS) and to evaluate the reliability of the scale. A team of 10 physicians based in Beijing, China, consisting of 1 principal investigator and 9 independent raters, assessed a pool of subject images. Read More

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Incident detection of human papillomavirus - a prospective follow-up study among Tanzanian women with focus on HIV status.

Int J Infect Dis 2021 Jul 9. Epub 2021 Jul 9.

Department of Virus, Lifestyle and Genes, Danish Cancer Society Research Center, Copenhagen, Denmark; Department of Gynaecology, Rigshospitalet, Copenhagen University Hospital, Denmark.

Background: The causative role of human papillomavirus (HPV) in cervical carcinogenesis is well established. Despite this, prospective studies examining high-risk (HR) HPV acquisition among adult women in HIV prevalent settings are limited.

Methods: We conducted a prospective study among women (25-60 years) attending cervical cancer screening in Tanzania. Read More

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Predictors of HPV Seropositivity in Appalachian Women age 18-26 Years.

Sex Transm Dis 2021 Mar 23. Epub 2021 Mar 23.

1The Department of Family and Community Medicine, Penn State Hershey Medical Center, Hershey, PA 17033 2The Center for Biostatistics, Department of Biomedical Informatics, College of Medicine, The Ohio State University, 320G, 1800 Cannon Drive, Columbus, OH 43210 USA; 3Department of Family Medicine, The Ohio State University Wexner Medical Center, Columbus, OH 4The Institute for Behavioral Medicine Research, The Ohio State University Wexner Medical Center, Room 112, 460 Medical Center Drive, Columbus, OH, 43210 USA; 5Department of Psychiatry & Behavioral Health., College of Medicine. The Ohio State University, Columbus, OH 6Department of Obstetrics and Gynecology, The Ohio State University Wexner Medical Center, Columbus, OH 7Departemnt of Internal Medicine, College of Medicine. The Ohio State University, Columbus Ohio 8Department of Family Medicine, Obstetrics and Gynecology, Women's Studies, Bioengineering, University of Michigan, Ann Arbor, MI.

Background: Key informants of the Appalachian community questioned whether their unique environmental stressors would alter their immune response to HPV infections. The primary aim of this study is to determine predictors of HPV seroprevalence to at least one of the four vaccine related HPV types prior to vaccination using a psychoneuroimmunologic model in Appalachian women.

Method: Women ages 18-26 years old (n=185) who had not received HPV vaccination provided cervical HPV DNA and blood samples. Read More

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Long-term cervical precancer outcomes after a negative DNA- or RNA-based human papillomavirus test result.

Am J Obstet Gynecol 2021 Jun 1. Epub 2021 Jun 1.

Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada; British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada; Lower Mainland Laboratories, Vancouver, British Columbia, Canada. Electronic address:

Background: Cervical cancer, a preventable disease associated with the human papillomavirus, is responsible for significant morbidity and mortality globally. Primary human papillomavirus testing is more sensitive in detecting precancerous cervical lesions than cytologic screening and can be conducted using either DNA- or RNA-based assays. Screening programs must select the most appropriate assay from several available assays for their population. Read More

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A Novel Thyroid Ultrasound Proficiency Metric Designed Through a Multidisciplinary Delphi Approach.

Am Surg 2021 Apr 28:31348211011151. Epub 2021 Apr 28.

Section of Endocrine Surgery, Division of General Surgery, Department of Surgery, 2358University of Virginia, Charlottesville, VA, USA.

Background: Thyroid ultrasounds extend surgeons' outpatient capabilities and are essential for operative planning. However, most residents are not formally trained in thyroid ultrasound. The purpose of this study was to create a novel thyroid ultrasound proficiency metric through a collaborative Delphi approach. Read More

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Long term results of follow-up after HPV self-sampling with devices Qvintip and HerSwab in women non-attending cervical screening programme.

Radiol Oncol 2021 01 6;55(2):187-195. Epub 2021 Jan 6.

University Medical Centre Maribor, Maribor, Slovenia.

Background: We are presenting the results of the Slovenian human papillomaviruses (HPV) self-sampling pilot study in colposcopy population of National Cervical Cancer Screening Programme ZORA for the first time. One-year and four-year follow-up results are presented for two different self-sampling devices.

Participants And Methods: A total of 209 women were enrolled in the study at colposcopy clinic. Read More

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January 2021

The treatment strategy of patients with positive margins after cervical cold knife conization-A 7-year retrospective study in China.

Int J Gynaecol Obstet 2021 Mar 23. Epub 2021 Mar 23.

Department of Gynecology, Xiangya Hospital, Central South University, Changsha, China.

Objective: To explore treatment strategies for patients with positive margins after cervical cold knife conization (CKC) by estimating the risk of residual or recurrent CIN2 or worse (CIN2+).

Methods: A retrospective study included 569 patients receiving CKC for CIN3 in Xiangya Hospital from January 2013 to December 2017. Demographic characteristics and test results were obtained before CKC, after CKC, at 6, 12, and 24 months, then annually thereafter. Read More

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Clinical and Analytical Evaluation of the Alinity m HR HPV Assay within the VALGENT-3 Framework.

J Clin Microbiol 2021 05 19;59(6). Epub 2021 May 19.

Unit of Cancer Epidemiology/Belgian Cancer Centre, Scientific Institute of Public Health, Brussels, Belgium

Only clinically validated human papillomavirus (HPV) tests should be used in cervical cancer screening. VALGENT provides a framework to validate new HPV tests. In the VALGENT-3 study, the clinical accuracy of the recently launched Abbott Alinity m HR HPV assay (Alinity m) to detect cervical precancerous lesions was assessed against the standard comparator test (Hybrid Capture 2; HC2) and against two previously validated alternative comparator tests (Abbott RealTie HR HPV and Roche cobas 4800 assays). Read More

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Detection of sexually transmitted pathogens and co-infection with human papillomavirus in women residing in rural Eastern Cape, South Africa.

PeerJ 2021 3;9:e10793. Epub 2021 Mar 3.

Department of Pathology, Faculty of health sciences, University of Cape Town, Cape Town, South Africa.

Background: South African women of reproductive age have a high burden of sexually transmitted infections (STIs), including human papillomavirus (HPV) infection. However, there is limited information on the prevalence of sexually transmitted pathogens in women from rural Eastern Cape Province, South Africa. The study aims at determining the prevalence of sexually transmitted pathogens and co-infection with high-risk (HR) HPV among women from rural Eastern Cape Province, South Africa. Read More

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Diagnostic accuracy of cervical cancer screening and screening-triage strategies among women living with HIV-1 in Burkina Faso and South Africa: A cohort study.

PLoS Med 2021 03 4;18(3):e1003528. Epub 2021 Mar 4.

London School of Hygiene & Tropical Medicine, London, United Kingdom.

Background: Cervical cancer screening strategies using visual inspection or cytology may have suboptimal diagnostic accuracy for detection of precancer in women living with HIV (WLHIV). The optimal screen and screen-triage strategy, age to initiate, and frequency of screening for WLHIV remain unclear. This study evaluated the sensitivity, specificity, and positive predictive value of different cervical cancer strategies in WLHIV in Africa. Read More

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Comparison of Aptima and hybrid capture-2 HPV tests and Pap test in the referral population in Japan.

J Med Virol 2021 Aug 25;93(8):5076-5083. Epub 2021 Mar 25.

Department of Obstetrics and Gynecology, Kanazawa Medical University, Uchinada, Japan.

The Aptima human papillomavirus (HPV) test (APTIMA) detects E6-E7 mRNA in abnormal cells in the uterine cervix. To investigate the accuracy of APTIMA for cervical cancer screening in Japan, 423 subjects, mostly referrals with abnormal cytology or being followed up for cervical intraepithelial neoplasia (CIN)1, were screened using two HPV tests, hybrid capture 2 (HC2) and APTIMA, and by the Pap test. Colposcopy was conducted in all subjects with a positive result in either test type. Read More

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Hybrid capture 2 and cobas® 4800: Comparison of performance of two clinically validated tests for human papillomavirus primary screening of cervical cancer.

J Med Screen 2021 Feb 10:969141321992820. Epub 2021 Feb 10.

Regional Cancer Prevention Laboratory - Institute for Cancer Research, Prevention and Clinical Network (ISPRO), Florence, Italy.

Objective: To compare, in a primary human papillomavirus screening setting, two different validated human papillomavirus tests, considering their analytical and clinical screening performances.

Methods: In Tuscany, a human papillomavirus screening program was implemented in 2013. Hybrid capture 2 (Qiagen) was used for testing until May 2016, when it was replaced by the cobas 4800 human papillomavirus test (Cobas; Roche). Read More

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February 2021

Anyplex II HPV test in detection and follow-up after surgical treatment of CIN2+ lesions.

J Med Virol 2021 Feb 10. Epub 2021 Feb 10.

Division of Laboratory Medicine, European Institute of Oncology IRCCS, Milan, Italy.

Human papillomavirus (HPV) tests differ for technology, targets, and information on the genotype and viral load. In this study, we evaluated the performance of the Seegene Anyplex II HPV HR (Anyplex) assay in the detection of cervical intraepithelial lesions (CIN) and as a test-of-cure in the follow-up after surgical treatment. One hundred and sixty-seven women referred to the European Institute of Oncology, Milan, for surgical treatment of CIN2+ were enrolled. Read More

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February 2021

Split-type electrochemiluminescent gene assay platform based on gold nanocluster probe for human papillomavirus diagnosis.

Biosens Bioelectron 2021 Apr 28;178:113044. Epub 2021 Jan 28.

Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou, 350004, China. Electronic address:

Persistent high-risk human papillomavirus (HPV) infection is the leading cause of cervical cancer. Efficient detection of HPV16 E7 is necessary for early diagnosis and cure of the disease. Here, a novel and high-performance Au nanocluster (AuNC) probe-based split-type electrochemiluminescent (ECL) assay platform has been established to detect these oncogenes, in which the nucleic acid hybridization assay and the ECL measurements are performed independently. Read More

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Development of a Novel Multiple Cross-Linking Spiral Amplification for Rapid and Sensitive Detection of HPV16 DNA.

J Microbiol Biotechnol 2021 Apr;31(4):610-620

Department of Otolaryngology Head and Neck Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, P.R. China.

PCRThere has been increasing interest in the head and neck squamous cell carcinoma (HNSCC) that is caused by high-risk human papillomavirus (HR-HPV) and has posed a significant challenge to Otolaryngologists. A rapid, sensitive, and reliable method is required for the detection of HR-HPV in clinical specimens to prevent and treat HPV-induced diseases. In this study, a multiple cross-linking spiral amplification (MCLSA) assay was developed for the visual detection of HPV-16. Read More

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Agreement between careHPV and hybrid capture 2 in detecting high-risk HPV in women in Tanzania.

Acta Obstet Gynecol Scand 2021 04 13;100(4):786-793. Epub 2021 Feb 13.

Department of Gynecology and Obstetrics, Odense University Hospital, Odense, Denmark.

Introduction: Visual inspection of the cervix with acetic acid is used to control the burden of cervical cancer in low- and middle-income countries. This method has some limitations and HPV DNA testing may be an alternative, but it is expensive and requires a laboratory setup. Cheaper and faster HPV tests have been developed. Read More

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Acceptability of self- collection for human papillomavirus detection in the Eastern Cape, South Africa.

PLoS One 2020 10;15(11):e0241781. Epub 2020 Nov 10.

Division of Medical Virology, Department of Pathology, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa.

Human papillomavirus (HPV) testing on vaginal self-collected and cervical clinician-collected specimens shows comparable performance. Self-sampling on FTA cards is suitable for women residing in rural settings or not attending regular screening and increases participation rate in the cervical cancer screening programme. We aimed to investigate and compare high-risk (HR)-HPV prevalence in clinician-collected and self-collected genital specimens as well as two different HPV tests on the clinician collected samples. Read More

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January 2021

Efficient Sequencing, Assembly, and Annotation of Human KIR Haplotypes.

Front Immunol 2020 9;11:582927. Epub 2020 Oct 9.

Center for International Blood and Marrow Transplant Research, Minneapolis, MN, United States.

The homology, recombination, variation, and repetitive elements in the natural killer-cell immunoglobulin-like receptor (KIR) region has made full haplotype DNA interpretation impossible in a high-throughput workflow. Here, we present a new approach using long-read sequencing to efficiently capture, sequence, and assemble diploid human KIR haplotypes. Probes were designed to capture KIR fragments efficiently by leveraging the repeating homology of the region. Read More

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Design and feasibility of a novel program of cervical screening in Nigeria: self-sampled HPV testing paired with visual triage.

Infect Agent Cancer 2020 14;15:60. Epub 2020 Oct 14.

Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, Rockville, USA.

Background: Accelerated global control of cervical cancer would require primary prevention with human papillomavirus (HPV) vaccination in addition to novel screening program strategies that are simple, inexpensive, and effective. We present the feasibility and outcome of a community-based HPV self-sampled screening program.

Methods: In Ile Ife, Nigeria, 9406 women aged 30-49 years collected vaginal self-samples, which were tested for HPV in the local study laboratory using Hybrid Capture-2 (HC2) (Qiagen). Read More

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October 2020

Comprehensive Cervical Cancer Prevention in Tanzania (CONCEPT) study: Cohort profile.

BMJ Open 2020 09 18;10(9):e038531. Epub 2020 Sep 18.

Department of Clinical Research, University of Southern Denmark, Odense, Denmark

Purpose: Cervical cancer is a major cause of death among women in Eastern Africa, and the distribution of human papillomavirus (HPV) according to HIV status is inadequately characterised in this region. In order to guide future cervical cancer preventive strategies that involve HPV testing, the Comprehensive Cervical Cancer Prevention in Tanzania (CONCEPT) study was established in 2015. The CONCEPT cohort aims to investigate the natural history of HPV and determine acquisition and persistence patterns of high-risk (HR) HPV among HIV-positive and HIV-negative women. Read More

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September 2020

Screening strategies for the detection of anal high-grade squamous intraepithelial lesions in women living with HIV.

AIDS 2020 12;34(15):2249-2258

Department of Obstetrics and Gynecology, Boston University School of Medicine, Boston, Massachusetts, USA.

Objective: HIV-infected women (WLHIV) have more than 10-fold higher risk for squamous cell cancer of the anus. Experts suggest cytology-based strategies developed for cervical cancer screening may prevent anal cancer by detecting anal cytologic or histological high-grade squamous intraepithelial lesion (hHSIL) for treatment. Currently, there is no consensus on anal-hHSIL screening strategies for WLHIV. Read More

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December 2020

Permanent Bilateral Carotid Filters for Stroke Prevention in Atrial Fibrillation.

Curr Cardiol Rep 2020 09 10;22(11):144. Epub 2020 Sep 10.

Population Health Research Institute, McMaster University, Hamilton, Ontario, Canada.

Purpose Of Review: A novel permanent carotid filter device for percutaneous implantation was developed for the purpose of stroke prevention. In this review, we cover rationale, existing preclinical and clinical data, and potential future directions for research using such a device.

Recent Findings: The Vine™ filter was assessed for safety in sheep and in 2 observational human studies, the completed CAPTURE 1 (n = 25) and the ongoing CAPTURE 2 (planned n = 100). Read More

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September 2020

Negative Roche cobas HPV testing in cases of biopsy-proven invasive cervical carcinoma, compared with Hybrid Capture 2 and liquid-based cytology.

J Am Soc Cytopathol 2021 Mar-Apr;10(2):128-134. Epub 2020 Aug 13.

Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, New York.

Introduction: The objective of this study was to conduct a retrospective analysis of results of cytology and Roche cobas (RC) and Hybrid Capture 2 (HC2) human papillomavirus (HPV) screening tests in cases of biopsy-proven invasive cervical carcinoma.

Materials And Methods: The clinical data were obtained at a university hospital in New York, NY, between 2004 and 2017. Results of cytology, reported per Bethesda classification system, and HPV screening in 177 identified cases with cytology and biopsy-proven diagnosis of cervical carcinoma were included in the analysis. Read More

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Evaluation of a Chip-Based, Point-of-Care, Portable, Real-Time Micro PCR Analyzer for the Detection of High-Risk Human Papillomavirus in Uterine Cervix in India.

JCO Glob Oncol 2020 07;6:1147-1154

Indian Council of Medical Research-Indian Cancer Research Consortium, New Delhi, India.

Purpose: Currently available human papillomavirus (HPV) detection devices are expensive, requiring a continuous power supply, high-priced reagents, skilled laboratory personnel, and infrastructure. These make it difficult to implement primary HPV screening in high-risk (HR) populations, particularly in low-income settings such as in India. The objective of our study was to evaluate the diagnostic performance of a point-of-care, portable, battery-operated device called Truenat, which detects 4 HR HPV genotypes (16, 18, 31, and 45), as a potentially cost-effective alternative to conventional HPV diagnostic tests. Read More

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Immediate histologic correlation in women with atypical squamous cells of undetermined significance cytology and positive high-risk HPV: A retrospective review of 6000 cases in a large academic women's hospital.

Cancer Cytopathol 2020 11 8;128(11):852-859. Epub 2020 Jul 8.

Department of Pathology, Magee-Womens Hospital, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania.

Background: Previous studies of the histologic correlation in women who have atypical squamous cells of undetermined significance cytology (ASC-US) and are positive for high-risk human papillomavirus (hrHPV+) have predominantly utilized the Hybrid Capture 2 (HC2) test, whereas the use of other US Food and Drug Administration (FDA)-approved methods is relatively limited.

Methods: Cases of ASC-US/hrHPV+ that were tested using HC2, Cervista, or Aptima were retrieved, and the immediate histologic correlations were analyzed.

Results: Overall, 53. Read More

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November 2020

High-risk Human Papillomavirus Testing for Triage of Women with Previous Cytological Abnormalities from the Vale do Ribeira Region.

Rev Bras Ginecol Obstet 2020 Jun 30;42(6):340-348. Epub 2020 Jun 30.

Departamente of Pathological Anatomy, Instituto Adolfo Lutz, São Paulo, SP, Brazil.

Objective:  To evaluate the performance of the hybrid capture 2 (HC2) high-risk papillomavirus (hrHPV) assay and cytological test in women with previous abnormalities, to detect cervical intraepithelial neoplasia grade 2 or worse (≥ CIN 2).

Methods:  A cytological test and HC2 (Qiagen, Gaithersburg, Maryland, EUA) for hrHPV were conducted in 359 liquid-based (Sure Path, Becton Dickinson, TriPath Imaging, Burlington, NC, USA) samples collected from women from the Vale do Ribeira Region, during July 2013 and September 2015 with previous cytology classified as atypical squamous cells of undetermined significance (ASC-US), low-grade squamous intraepithelial lesion (LSIL), atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesions (ASC-H), and atypical glandular cells (AGC). The histopathological examination was conducted in 179 women. Read More

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Prevalence of high-risk human papillomavirus after HPV-vaccination in Denmark.

Int J Cancer 2020 12 29;147(12):3446-3452. Epub 2020 Jun 29.

Department of Public Health Programmes, Randers Regional Hospital, Randers, Denmark.

Vaccination against human papillomavirus (HPV) has been introduced as a public health initiative in many countries, including Denmark since October 2008. It is important to monitor postimplementation effectiveness of HPV-vaccination at the population-level. We studied HPV-prevalence after first invitation to screening at age 23 years in women offered the quadrivalent HPV-vaccine at the age of 14 years. Read More

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December 2020

A study of type-specific HPV natural history and implications for contemporary cervical cancer screening programs.

EClinicalMedicine 2020 May 25;22:100293. Epub 2020 Apr 25.

Division of Cancer Epidemiology and Genetics, National Cancer Institute, Rockville, MD, United States.

Background: HPV testing is replacing cytology for cervical cancer screening because of greater sensitivity and superior reassurance following negative tests for the dozen HPV genotypes that cause cervical cancer. Management of women testing positive is unresolved. The need for identification of individual HPV genotypes for clinical use is debated. Read More

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HPV types, cervical high-grade lesions and risk factors for oncogenic human papillomavirus infection among 3416 Tanzanian women.

Sex Transm Infect 2021 02 8;97(1):56-62. Epub 2020 Apr 8.

Department of Obstetric and Gynaecology, Odense University Hospital, Odense, Denmark.

Objective: The objective of the present study was to assess the prevalence and type-specific distribution of cervical high-risk (HR) human papillomavirus (HPV) among women with normal and abnormal cytology, and to describe risk factors for HR HPV among HIV-positive and HIV-negative women in Tanzania.

Methodology: A cross-sectional study was conducted in existing cervical cancer screening clinics in Kilimanjaro and Dar es Salaam. Cervical specimens were obtained from women aged 25-60 years. Read More

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February 2021