29,446 results match your criteria capillary electrophoresis

Quantitative microcapillary electrophoresis immunoassay (mCE IA) for end-to-end analysis of pertactin within in-process samples and Quadracel® vaccine.

J Pharm Biomed Anal 2021 Jul 26;204:114284. Epub 2021 Jul 26.

Analytical Sciences Toronto, Sanofi Pasteur Ltd., Toronto, ON, M2R 3T4, Canada. Electronic address:

Protein concentration is an important attribute in the production of subunit or component-based vaccine antigens. Rigorous monitoring of protein concentration is required to identify potential areas for yield improvement. The current GMP method for quantitation is the plate-based ELISA which requires numerous hands-on steps and has low sensitivity in comparison to new microfluidic systems. Read More

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PolyG-DS: An ultrasensitive polyguanine tract-profiling method to detect clonal expansions and trace cell lineage.

Proc Natl Acad Sci U S A 2021 Aug;118(31)

Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98195;

Polyguanine tracts (PolyGs) are short guanine homopolymer repeats that are prone to accumulating mutations when cells divide. This feature makes them especially suitable for cell lineage tracing, which has been exploited to detect and characterize precancerous and cancerous somatic evolution. PolyG genotyping, however, is challenging because of the inherent biochemical difficulties in amplifying and sequencing repetitive regions. Read More

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Immunotyping Provides Equivalent Results to Immunofixation in a Population with a High Prevalence of Monoclonal Gammopathies.

J Appl Lab Med 2021 Jul 30. Epub 2021 Jul 30.

Clinical Chemistry Service, Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, NY.

Background: Serum immunofixation (IF) is a common laboratory test used to diagnose and monitor patients with monoclonal gammopathies. Similarly, immunotyping (IT) by capillary electrophoresis can confirm the presence of a monoclonal protein (M-protein) and determine its isotype. The goal of this study was to compare the ability of IT and IF to detect M-proteins. Read More

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Non-targeted LC-MS and CE-MS for biomarker discovery in bioreactors: Influence of separation, mass spectrometry and data processing tools.

Sci Total Environ 2021 Jul 17;798:149012. Epub 2021 Jul 17.

Department of Chemistry, Aalen University, D-73430 Aalen, Germany. Electronic address:

Liquid separation coupled to mass spectrometry is often used for non-targeted analyses in various fields, such as metabolomics. However, the combination of non-standardized methods, various mass spectrometers (MS) and processing tools for data evaluation affect biomarker discovery potentially. Here, we present a comprehensive study of these factors based on non-targeted liquid chromatography coupled to time-of-flight (TOF) and Orbitrap MS and capillary zone electrophoresis to Orbitrap analyses of the same bioreactor samples, describing the correlation of its gas yield with changing feature signal intensity. Read More

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Poly(carboxyethyl acrylate-co-ethylene glycol dimethacrylate) precursor monolith with bonded octadecyl ligands for use in reversed-phase capillary electrochromatography.

Electrophoresis 2021 Jul 29. Epub 2021 Jul 29.

Department of Chemistry, Oklahoma State University, Stillwater, OK, 74078-3071, USA.

A carboxy precursor monolithic column, namely poly(carboxy ethyl acrylate-co-ethylene glycol dimethacrylate was first produced in a 100 μm i.d. fused-silica capillary and subsequently surface bonded with n-octadecyl (C ) ligands by a post-polymerization functionalization process with octadecylamine in the presence of N,N´-dicyclohexylcarbodiimide. Read More

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Microchip Electrophoresis Assay for Calmodulin Binding Proteins.

J Sep Sci 2021 Feb 7;44(4):895-902. Epub 2020 Dec 7.

Department of Chemistry, University of Kansas, Lawrence, Kansas, USA.

The calcium signaling protein calmodulin regulates numerous intracellular processes. We introduce a sensitive microchip assay to separate and detect calmodulin binding proteins. The assay utilizes an optimized microchip electrophoresis protein separation platform with laser-induced fluorescence detection. Read More

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February 2021

Analysis of amino acids in cell culture supernatant using capillary electrophoresis with contactless conductivity detection.

Electrophoresis 2021 Jul 26. Epub 2021 Jul 26.

Division of Applied Physical Chemistry, Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Teknikringen 36, Stockholm, SE-100 44, Sweden.

CE-C D methods for the analysis of amino acids (AAs) are presented. Combining the results from two methods with acetic acid and cyclodextrin based BGEs, 20 proteinogenic AAs could be analysed using CE. CE-C D was also, for the first time, applied to analyse free AAs in samples of mammalian cell culture supernatant. Read More

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A Novel α-Globin Chain Variant, Hb Nanchang [: c.46G>A, Codon 15 (GT>GT) (Gly→Ser)], Detected by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.

Hemoglobin 2021 Jul 26:1-4. Epub 2021 Jul 26.

Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, People's Republic of China.

Here we report a new α chain variant accidentally discovered during Hb A measurement by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) that revealed the presence of a variant α chain with a mass of 15155 Da. However, this hemoglobin (Hb) variant cannot be detected by the first-line methods such as cation exchange high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Sanger sequencing confirmed the presence of a heterozygous missense mutation [: c. Read More

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Hemoglobin Fukuoka caused unexpected hemoglobin A results: A case report.

World J Clin Cases 2021 Jul;9(20):5568-5574

Department of Clinical Medical Laboratory, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen 518107, Guangdong Province, China.

Background: Glycated hemoglobin (Hb) (HbA) is an indicator that is used to diagnose and monitor the treatment of diabetes. Many factors can affect the detection of HbA. One of the most important of these factors is the Hb variant. Read More

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Recent Applications of Capillary Electrophoresis in the Determination of Active Compounds in Medicinal Plants and Pharmaceutical Formulations.

Molecules 2021 Jul 7;26(14). Epub 2021 Jul 7.

Department of Otolaryngology and Oncology, Faculty of Medicine, L. Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Skłodowskiej Curie 9 Street, PL-85094 Bydgoszcz, Poland.

The present review summarizes scientific reports from between 2010 and 2019 on the use of capillary electrophoresis to quantify active constituents (i.e., phenolic compounds, coumarins, protoberberines, curcuminoids, iridoid glycosides, alkaloids, triterpene acids) in medicinal plants and herbal formulations. Read More

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Endpoint PCR coupled with capillary electrophoresis (celPCR) provides sensitive and quantitative measures of environmental DNA in singleplex and multiplex reactions.

PLoS One 2021 23;16(7):e0254356. Epub 2021 Jul 23.

Department of Zoology, University of Innsbruck, Innsbruck, Austria.

The use of sensitive methods is key for the detection of target taxa from trace amounts of environmental DNA (eDNA) in a sample. In this context, digital PCR (dPCR) enables direct quantification and is commonly perceived as more sensitive than endpoint PCR. However, endpoint PCR coupled with capillary electrophoresis (celPCR) potentially embodies a viable alternative as it quantitatively measures signal strength after PCR in Relative Fluorescence Units (RFU). Read More

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Separation of short and medium-chain fatty acids using capillary electrophoresis with indirect photometric detection: Part I: Identification of fatty acids in rat feces.

Electrophoresis 2021 Jul 20. Epub 2021 Jul 20.

Neuroscience Institute and Department of Biology, Georgia State University, Atlanta, USA.

Short and medium-chain fatty acids (SMCFA) are known as essential metabolites found in gut microbiota that function as modulators in the development and progression of many inflammatory conditions as well as in the regulation of cell metabolism. Currently, there are few simple and low-cost analytical methods available for the determination of SMCFA. This report focuses on SMCFA analysis utilizing capillary electrophoresis with indirect photometric detection (CE-IPD). Read More

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Capillary electrophoresis method for analysis of inorganic and organic anions related to habitability and the search for life.

Electrophoresis 2021 Jul 20. Epub 2021 Jul 20.

Jet Propulsion Laboratory, California Institute of Technology, 4800 Oak Grove Drive, Pasadena, California, 91109, USA.

In situ missions of exploration require analytical methods that are capable of detecting a wide range of molecular targets in complex matrices without a priori assumptions of sample composition. Furthermore, these methods should also minimize the number of reagents needed and any sample preparation steps. We have developed a method for the detection of metabolically relevant inorganic and organic anions that is suitable for implementation on in situ spaceflight missions. Read More

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A Novel Urinary Proteomics Classifier for Non-Invasive Evaluation of Interstitial Fibrosis and Tubular Atrophy in Chronic Kidney Disease.

Proteomes 2021 Jul 13;9(3). Epub 2021 Jul 13.

Department of Nephrology, Angiology and Rheumatology, Klinikum Bayreuth GmbH, 95447 Bayreuth, Germany.

Non-invasive urinary peptide biomarkers are able to detect and predict chronic kidney disease (CKD). Moreover, specific urinary peptides enable discrimination of different CKD etiologies and offer an interesting alternative to invasive kidney biopsy, which cannot always be performed. The aim of this study was to define a urinary peptide classifier using mass spectrometry technology to predict the degree of renal interstitial fibrosis and tubular atrophy (IFTA) in CKD patients. Read More

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Experimental and computational study on the enantioseparation of four chiral fluoroquinolones by capillary electrophoresis with sulfated-β-cyclodextrin as chiral selector.

Chirality 2021 Jul 18. Epub 2021 Jul 18.

School of Pharmacy, Binzhou Medical University, Yantai, China.

In this work, enantioseparation of four chiral fluoroquinolones (FQs), namely, ofloxacin, gemifloxacin, lomefloxacin, and gatifloxacin, was achieved by capillary electrophoresis with sulfated-β-cyclodextrin (S-β-CD) as chiral selector. Factors affecting the enantiomeric resolution, such as the concentrations of S-β-CD, BGE pH conditions, and the buffer types and concentrations, were optimized and discussed. A BGE consisting of 30 g/L S-β-CD and 30-mM phosphate at pH 4. Read More

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Glycoprotein analysis using lectin microcolumns and capillary electrophoresis: Characterization of alpha-acid glycoprotein by combined separation methods.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Jul 9;1179:122855. Epub 2021 Jul 9.

Department of Chemistry, University of Nebraska, Lincoln, NE 68588, USA. Electronic address:

Separations based on combinations of 2.1 mm I.D. Read More

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Structural model and functional properties of an exo-polygalacturonase from Neosartorya glabra.

Int J Biol Macromol 2021 Jul 15;186:909-918. Epub 2021 Jul 15.

Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil; Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo 14040-901, Brazil. Electronic address:

A purified exo-polygalacturonase of Neosartorya glabra (EplNg) was successfully characterized. EplNg native presented 68.2 kDa, with 32% carbohydrate content. Read More

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Biosynthesis of UDP-2-acetamido-4-formamido-2,4,6-trideoxy-hexose by WekG, WekE, WekF, and WekD: Enzymes in the Wek pathway responsible for O-antigen Assembly in Escherichia coli O119.

Carbohydr Res 2021 Sep 7;507:108388. Epub 2021 Jul 7.

TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, China; Key Laboratory of Microbial Functional Genomics, Tianjin, China; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin, China. Electronic address:

Considering the importance of bacterial glycoconjugates on virulence and host mimicry, there is a need to better understand the biosynthetic pathways of these unusual sugars to identify critical targets involved in bacterial pathogenesis. In this report, we describe the cloning, overexpression, purification, and biochemical characterization of the four central enzymes in the biosynthesis pathway for UDP-2-acetamido-4-formamido-2,4,6-trideoxy-hexose, WekG, WekE, WekF, and WekD. Product peaks from enzyme-substrate reactions were detected by using a combination of capillary electrophoresis (CE) and electrospray ionization-mass spectrometry (ESI-MS). Read More

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September 2021

Long-term intake of Lactobacillus paracasei KW3110 prevents age-related circadian locomotor activity and changes in gut metabolism in physiologically aged mice.

Exp Gerontol 2021 Jul 13;153:111477. Epub 2021 Jul 13.

Department of Inflammation and Immunosenescence, Geroscience Research Center, Research Institute, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8511, Japan.; Department of Aging Research, Nagoya University Graduate School of Medicine, Nagoya, Aichi, 466-8550, Japan. Electronic address:

Aging involves age-progressive loss of physiological functions in organs and tissues. We previously showed that Lactobacillus paracasei KW3110 suppressed age-related inflammation and prevented age-related retinal ganglion cell (RGC) loss. As RGCs mediate biological behaviors associated with responses to ambient light, we assessed whether L. Read More

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Potential and applications of capillary electrophoresis for analyzing traditional Chinese medicine: a critical review.

Analyst 2021 Aug 16;146(15):4724-4736. Epub 2021 Jul 16.

Academician Workstation, Changsha Medical University, Changsha 410219, China.

Capillary electrophoresis (CE) presents a promising possibility for analyzing traditional Chinese medicine (TCM) due to its low reagent consumption, high analysis speed, and enhanced efficiency. Herein we review the employment of CE for analyzing the effective components in TCM and identifying TCM via a fingerprint. Furthermore, we discuss the application of state-of-the-art capillary electrophoresis modes for screening enzyme inhibitors and investigating the interactions between TCM and plasma proteins. Read More

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Polyhalogenated Bicyclo[1.1.1]pentane-1,3-dicarboxylic Acids.

J Org Chem 2021 Jul 16. Epub 2021 Jul 16.

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo nám. 2, 160 00 Prague 6, Czech Republic.

Herein we report the highly selective radical chlorination of 2,2-difluorobicyclo[1.1.1]pentane-1,3-dicarboxylic acid. Read More

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Rapid and comprehensive monoclonal antibody Characterization using microfluidic CE-MS.

J Pharm Biomed Anal 2021 Jul 7;204:114251. Epub 2021 Jul 7.

CMC Analytical, GlaxoSmithKline, 1250 S. Collegeville Road, UP 1400, Collegeville, PA, 19426, USA.

The identification and control of monoclonal antibody (mAb) critical quality attributes (CQAs) is a key component of quality by design (QbD). In this work, rapid peptide mapping and native intact charge variants analysis have been developed to comprehensively characterize and monitor mAb CQAs using a microfluidic capillary electrophoresis - mass spectrometry (CE-MS) platform. The ultrafast peptide mapping simultaneously analyzed multiple CQAs, including protein primary structure, oxidation, deamidation, succinimide, C-terminal lysine (Lys) clipping, N-terminal cyclization, and glycosylation. Read More

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The evaluation of the chemical quality and UV overall components dissolution consistency of preparation.

Anal Methods 2021 Jul 15. Epub 2021 Jul 15.

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110032, China.

In recent years, new chemical technology and chemical analysis methods have been widely used for the quality control of medicine to provide security for human life. However, for the increasingly popular traditional Chinese medicine (TCM) preparations, the traditional quality control technology has been challenged due the presence of multi-components. To solve this problem, this study proposed a comprehensive evaluation strategy from two aspects: chemical quality and in vitro dissolution consistency. Read More

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Sensitivity of Capillary Electrophoresis to Detect Monoclonal Immunoglobulins: a Report of Two Cases and a Systematic Review.

Clin Lab 2021 Jul;67(7)

Background: The detection of monoclonal immunoglobulins (MIg) is a key element in the diagnosis of monoclonal gammopathies.

Methods: Here we report two cases of high concentration serum IgM-kappa MIgs (6.4 g/L and 6. Read More

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Comparison of Four Methods for Streptococcus pneumoniae Capsular Typing.

Clin Lab 2021 Jul;67(7)

Background: Pneumococcal capsular-type identification is essential for monitoring the epidemiology of pneumococcal infections and for establishing the effectiveness of pneumococcal vaccines. The objective of this work was to compare the accuracy of four methods of Streptococcus pneumoniae capsular typing.

Methods: A prospective blind study was carried out at Donostia University Hospital (northern Spain) to determine the capsular types of 50 pneumococcal clinical isolates using four techniques: a) S. Read More

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Application of Multiple Short Tandem Repeat Loci for Rapid Diagnosis of Down Syndrome and Edward Syndrome.

Clin Lab 2021 Jul;67(7)

Background: Chromosomal diseases with chromosomal abnormalities are one of the most common genetic diseases in humans, including abnormal numbers and structural abnormalities. Patau syndrome (also known as trisomy 13), Edward syndrome (also called trisomy 18), and Down syndrome (also known as trisomy 21) are all clinically fatal diseases caused by abnormal numbers of autosomes. However, there is no reliable and effective cure for chromosomal diseases, mainly relying on fast and accurate prenatal diagnosis technology to reduce the rate of birth defects. Read More

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Comparison of Capillary Zone Electrophoresis in Greater Flamingos () and American Flamingos ().

J Avian Med Surg 2021 Jul;35(2):180-186

Laboklin GmbH & Co KG, 97688 Bad Kissingen, Germany.

Electrophoresis can be used to aid in the diagnosis of infectious diseases (eg, aspergillosis) in avian species. Reference intervals for blood plasma proteins of 2 different flamingo species ( and ) and their hybrids were calculated by capillary zone electrophoresis (CZE) and differences between these species, sexes, and age groups were evaluated. Lithium-heparinized plasma samples from 111 animals from a zoological collection were analyzed by CZE and statistically evaluated. Read More

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Online preconcentration of lysozyme in hen egg white using responsive polymer coating in CE.

J Sep Sci 2021 Jul 13. Epub 2021 Jul 13.

CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, P. R. China.

A mixed polymer brushes material based on poly (2-methyl-2-oxazoline)- and poly (acrylic acid)-coated capillary with switchable protein adsorption/desorption properties was applied for online preconcentration of lysozyme in hen egg white during capillary electrophoresis performance. First, lysozyme in simulated egg white was successfully online preconcentrated and the detection signal of lysozyme was amplified. Ovalbumin, ovomucoid, and conalbumin in egg white were verified show negligible interference on the online preconcentration of lysozyme according to the study on electroosmotic flow mobility. Read More

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The assessment of Pseudomonas aeruginosa lectin LecA binding characteristics of divalent galactosides using multiple techniques.

Glycobiology 2021 Jul 10. Epub 2021 Jul 10.

Department of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.

Pseudomonas aeruginosa is a widespread opportunistic pathogen that is capable of colonizing various human tissues and is resistant to many antibiotics. LecA is a galactose binding tetrameric lectin involved in adhesion, infection and biofilm formation. This study reports on the binding characteristics of mono- and divalent (chelating) ligands to LecA using different techniques. Read More

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Rapid chiral discrimination of oncometabolite dl-2-hydroxyglutaric acid using derivatization and field asymmetric waveform ion mobility spectrometry/mass spectrometry.

J Sep Sci 2021 Jul 13. Epub 2021 Jul 13.

Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.

2-Hydroxyglutaric acid is a chiral metabolite whose enantiomers specifically accumulate in different diseases. An enantiomeric excess of the d-form in biological specimens reflects the existence of various pathogenic mutations in cancer patients, however, conventional methods using gas or liquid chromatography and capillary electrophoresis had not been used for large clinical studies because they require multiple analytical instruments and a long run time to separate the enantiomers. Here, we present a rapid separation method for dl-2-hydroxyglutaric acid using a chiral derivatizing reagent and field asymmetric waveform ion mobility spectrometry/mass spectrometry, which requires a single analytical instrument and <1 s for the separation. Read More

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