Determination of 11 Cannabinoids in Biomass and Extracts of Different Varieties of Cannabis Using High-Performance Liquid Chromatography.
- Waseem Gul,
- Shahbaz W Gul,
- Mohamed M Radwan,
- Amira S Wanas,
- Zlatko Mehmedic,
- Ikhlas I Khan,
- Maged H M Sharaf,
- Mahmoud A ElSohly
J AOAC Int 2015 Nov-Dec;98(6):1523-8
ElSohly Laboratories, Inc., 5 Industrial Park Dr, Oxford, MS 38655, USA.
An HPLC single-laboratory validation was performed for the detection and quantification of the 11 major cannabinoids in most cannabis varieties, namely, cannabidiolic acid (CBDA), cannabigerolic acid (CBGA), cannabigerol (CBG), cannabidiol (CBD), tetrahydrocannabivarin (THCV), cannabinol (CBN), Δ9-trans-tetrahydrocannabinol (Δ9-THC), Δ8-trans-tetrahydrocannabinol (Δ8-THC), cannabicyclol (CBL), cannabichromene (CBC), and Δ9-tetrahydrocannabinolic acid-A (THCAA). The analysis was carried out on the biomass and extracts of these varieties. Methanol-chloroform (9:1, v/v) was used for extraction, 4-androstene-3,17-dione was used as the internal standard, and separation was achieved in 22. Read More