553 results match your criteria callus volume

Characterization of the Developing Lacunocanalicular Network During Fracture Repair.

JBMR Plus 2021 Sep 12;5(9):e10525. Epub 2021 Jul 12.

Institute for Biomechanics ETH Zurich Zurich Switzerland.

Fracture repair is a normal physiological response to bone injury. During the process of bony callus formation, a lacunocanalicular network (LCN) is formed de novo that evolves with callus remodeling. Our aim was the longitudinal assessment of the development and evolution of the LCN during fracture repair. Read More

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September 2021

Bone Union Assessment with Computed Tomography (CT) and Statistical Associations with Mechanical or Histological Testing: A Systematic Review of Animal Studies.

Calcif Tissue Int 2021 Aug 21. Epub 2021 Aug 21.

Department of Orthopaedics and Sports Medicine, Erasmus MC University Medical Centre, Doctor Molewaterplein 40, 3015GD, Rotterdam, The Netherlands.

Objective and accurate assessment of bone union after a fracture, arthrodesis, or osteotomy is relevant for scientific and clinical purposes. Bone union is most accurately imaged with computed tomography (CT), but no consensus exists about objective assessment of bone union from CT images. It is unclear which CT-generated parameters are most suitable for bone union assessment. Read More

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Biomimetic sponges improve functional muscle recovery following composite trauma.

J Orthop Res 2021 Jul 21. Epub 2021 Jul 21.

Department of Biomedical Engineering, Parks College of Engineering, Aviation, and Technology, Saint Louis University, St. Louis, Missouri, USA.

There is a dearth of therapies that are safe and effective for the treatment of volumetric muscle loss (VML), defined as the surgical or traumatic loss of muscle tissue, resulting in functional impairment. To address this gap in orthopedic care, we developed a porous sponge-like scaffold composed of extracellular matrix (ECM) proteins (e.g. Read More

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Strategies to Improve Bone Healing: Innovative Surgical Implants Meet Nano-/Micro-Topography of Bone Scaffolds.

Biomedicines 2021 Jun 28;9(7). Epub 2021 Jun 28.

Forschungsverbund BioMedizin Bielefeld/OWL FBMB e.V., Maraweg 21, 33617 Bielefeld, Germany.

Successful fracture healing is dependent on an optimal mechanical and biological environment at the fracture site. Disturbances in fracture healing (non-union) or even critical size bone defects, where void volume is larger than the self-healing capacity of bone tissue, are great challenges for orthopedic surgeons. To address these challenges, new surgical implant concepts have been recently developed to optimize mechanical conditions. Read More

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Effects of different vardenafil doses on bone healing in a rat fracture model.

Jt Dis Relat Surg 2021 11;32(2):313-322. Epub 2021 Jun 11.

SBÜ Bağcılar Eğitim ve Araştırma Hastanesi Ortopedi ve Travmatoloji Kliniği, 34200 Bağcılar, İstanbul, Türkiye.

Objectives: We aimed to investigate the radiological, biomechanical, histopathological, histomorphometric, and immunohistochemical effects of different doses of vardenafil on fracture healing.

Materials And Methods: Fifty-one rats were divided into three groups. Group V5 was given 5 mg/kg/day of vardenafil; Group V10 was given 10 mg/kg/day of vardenafil; and the control group was given the same volume of saline. Read More

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The effects of genotypes and media composition on callogenesis, regeneration and cell suspension culture of chamomile ( L.).

PeerJ 2021 24;9:e11464. Epub 2021 May 24.

Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture Faisalabad, Faisalabad, Pakistan.

Background: Chamomile is an important herb being used widely for medicinal purposes. Its multitherapeutic, cosmetic, and nutritional values have been established through years of traditional and scientific use and research. Increased use of medicinal plants necessitates rational use as well as sustainable production of such genetic resources. Read More

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Impaired soft and hard callus formation during fracture healing in diet-induced obese mice as revealed by 3D contrast-enhanced computed tomography imaging.

Bone 2021 09 14;150:116008. Epub 2021 May 14.

Prometheus - Division of Skeletal Tissue Engineering Leuven, KU Leuven, Leuven, Belgium; Department of Material Science and Engineering, KU Leuven, Leuven, Belgium; Biomechanics lab, Institute of Mechanics, Materials, and Civil Engineering, UCLouvain, Louvain-la-Neuve, Belgium; Institute for Experimental and Clinical Research, UCLouvain, Woluwe, Belgium. Electronic address:

The impact of diabetes mellitus on bone fracture healing is clinically relevant as the patients experience delayed fracture healing. Even though efforts have been made to understand the detrimental effects of type 2 diabetes mellitus (T2DM) on the fracture healing process, the exact mechanisms causing the pathophysiological outcomes remain unclear. The aim of this study was to assess alterations in bone fracture healing (tibial fracture surgery, intramedullary pinning) of diet-induced obese (DIO) mice, and to investigate the in vitro properties of osteochondroprogenitors derived from the diabetic micro-environment. Read More

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September 2021

Estrogen receptor α in mature osteoblasts regulates the late stage of bone regeneration.

Biochem Biophys Res Commun 2021 06 5;559:238-244. Epub 2021 May 5.

Division of Integrative Pathophysiology, Proteo-Science Center, Ehime University, Ehime, Japan; Department of Pathophysiology, Ehime University Graduate School of Medicine, Ehime, Japan. Electronic address:

Estrogen deficiency impairs fracture healing and homeostasis of bone tissue. OVX-induced estrogen deficiency in mice attenuates fracture healing and changes the expression ratio of estrogen receptor (ER) α and ERβ in callus during the process of fracture healing. Therefore, ERs may be involved in the regulation of fracture healing. Read More

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Chronic Intermittent Hypobaric Hypoxia Enhances Bone Fracture Healing.

Front Endocrinol (Lausanne) 2020 16;11:582670. Epub 2021 Feb 16.

Department of Physiology, Hebei Medical University, Shijiazhuang, China.

The effect of chronic intermittent hypobaric hypoxia (CIHH) on bone fracture healing is not elucidated. The present study aimed to investigate the role of CIHH on bone fracture healing and the mechanism. The Sprague-Dawley rats were randomly divided into the CIHH group and control group and monitored for 2, 4, or 8 weeks after femoral fracture surgery. Read More

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Effect of ovariectomy induced osteoporosis on metaphysis and diaphysis repair process.

Injury 2021 Jun 14;52(6):1300-1309. Epub 2021 Feb 14.

Department of Oral Anatomy and Developmental Biology, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan. Electronic address:

The fracture repair process is known to be delayed in postmenopausal women, under estrogen-deficient status. Osteoporotic fracture mainly occurs in the metaphyseal region of the long bone; however, most studies on fracture healing have focused on the diaphyseal region. In this study, we compared the repair process between metaphysis and diaphysis of ovariectomized (OVX) and Sham mice, and analyzed the effects of short-term estrogen administration in OVX mice. Read More

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Domain-independent simulation of physiologically relevant callus shape in mechanoregulated models of fracture healing.

J Biomech 2021 03 6;118:110300. Epub 2021 Feb 6.

Department of Mechanical Engineering and Mechanics, Lehigh University, Bethlehem, PA, USA.

Mechanoregulatory models have been used to predict the progression of bone fracture healing for more than two decades. However, many published studies share the same fundamental limitation: callus development proceeds within a pre-defined domain that both restricts and directs healing and leads to some non-physiologic healing patterns. To address this limitation, we added two spatial proximity functions to an existing mechanoregulatory model of fracture healing to control the localization of callus within the healing domain. Read More

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Repurposing denosumab for recalcitrant bone healing.

BMJ Case Rep 2021 Feb 8;14(2). Epub 2021 Feb 8.

Orthopedics, PD Hinduja National Hospital and Medical Research Centre, Mumbai, Maharashtra, India.

Fracture healing has four phases: haematoma formation, soft callus, hard callus and remodelling. Often, non-healing fractures have an arrest of one of these phases, which need resurgery. We have repurposed denosumab for impaired fracture healing cases to avoid surgical intervention. Read More

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February 2021

Axial Micromotion Locking Plate Construct Can Promote Faster and Stronger Bone Healing in an Ovine Osteotomy Model.

Front Bioeng Biotechnol 2020 15;8:593448. Epub 2021 Jan 15.

Trauma Center, Department of Orthopaedics and Traumatology, Shanghai General Hospital, Shanghai Jiaotong University, Shanghai, China.

Fixing bone fractures with controlled axial interfragmentary micromotion improves bone healing; however, the optimal type of implant construct for this purpose is still lacking. The present study describes a novel axial micromotion locking plate (AMLP) construct that allows axial interfragmentary micromotion of 0.3 or 0. Read More

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January 2021

Evaluation of Bone Healing Using Contrast-Enhanced Ultrasonography in Non-Operative Treatment of Tibial Fracture in a Puppy Dog.

Animals (Basel) 2021 Jan 23;11(2). Epub 2021 Jan 23.

Department of Veterinary Sciences, University of Messina, Viale Palatucci, 98168 Messina, Italy.

A 10-month-old mixed-breed male dog was presented with an oblique tibial fracture. The dog was treated with a Robert Jones-like bandage as a conservative approach, and was subjected to X-ray, B-mode, Color Doppler and contrast-enhanced ultrasound (CEUS) examinations during the fracture healing, in order to assess bone hemodynamic changes. B-mode, Power Doppler and CEUS examinations of the fracture gap were performed at 7, 20, 35, and 50 days post-trauma. Read More

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January 2021

Synthesis and Characterization of a Biocomposite Bone Bandage for Controlled Delivery of Bone-Active Drugs in Fracture Nonunions.

ACS Biomater Sci Eng 2020 05 6;6(5):2867-2878. Epub 2020 Apr 6.

Faculty of Medicine, Department of Clinical Sciences Lund, Orthopedics, Lund University, Lund 221 85, Sweden.

Fracture nonunions are common in orthopedics and their treatment often involves multiple surgical interventions. The aim of this study was to fabricate and characterize a gelatin-nano-hydroxyapatite membrane (GM)-based bone bandage for controlled delivery of bio-active molecules; recombinant human bone morphogenic protein-2 (rhBMP-2) and zoledronic acid (ZA) to promote osteoinduction and prevent callus resorption, respectively. In vitro cell-material interaction experiments using MC3T3 cells seeded on the GM indicated good biocompatibility. Read More

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Local injections of β-NGF accelerates endochondral fracture repair by promoting cartilage to bone conversion.

Sci Rep 2020 12 17;10(1):22241. Epub 2020 Dec 17.

Graduate Program in Oral and Craniofacial Sciences, School of Dentistry, University of California, San Francisco (UCSF), San Francisco, CA, USA.

There are currently no pharmacological approaches in fracture healing designed to therapeutically stimulate endochondral ossification. In this study, we test nerve growth factor (NGF) as an understudied therapeutic for fracture repair. We first characterized endogenous expression of Ngf and its receptor tropomyosin receptor kinase A (TrkA) during tibial fracture repair, finding that they peak during the cartilaginous phase. Read More

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December 2020

Once-weekly parathyroid hormone combined with ongoing long-term alendronate treatment promotes osteoporotic fracture healing in ovariectomized rats.

J Orthop Res 2021 Oct 25;39(10):2103-2115. Epub 2020 Dec 25.

Department of Orthopedics, Peking University Third Hospital, Beijing, China.

This study examined the effect of once-weekly parathyroid hormone (PTH) combined with alendronate upon osteoporotic fracture healing after long-term alendronate anti-osteoporosis therapy. Seventy-six 12-week-old female Sprague-Dawley rats were either sham operated or bilaterally ovariectomized (OVX). Following confirmation of osteoporosis 3 months after OVX, the remaining 64 animals received alendronate therapy. Read More

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October 2021

Reverse Dynamization Accelerates Bone-Healing in a Large-Animal Osteotomy Model.

J Bone Joint Surg Am 2021 Feb;103(3):257-263

Center for Limb Lengthening and Reconstruction, Nationwide Children's Hospital, Columbus, Ohio.

Background: Reverse dynamization is a mechanical manipulation regimen designed to accelerate bone-healing and remodeling. It is based on the hypothesis that a fracture that is initially stabilized less rigidly allows micromotion to encourage initial cartilaginous callus formation. Once substantial callus has formed, the stabilization should then be converted to a rigid configuration to prevent the disruption of neovascularization. Read More

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February 2021

Interleukin-6 (IL-6) deficiency enhances intramembranous osteogenesis following stress fracture in mice.

Bone 2021 02 10;143:115737. Epub 2020 Nov 10.

Department of Orthopaedic Surgery, Washington University in St. Louis, MO, United States of America; Department of Biomedical Engineering, Washington University in St. Louis, MO, United States of America.

Interleukin-6 (IL-6) is highly upregulated in response to skeletal injury, suggesting it plays a role in the inflammatory phase of fracture repair. However, the impact of IL-6 on successful repair remains incompletely defined. Therefore, we investigated the role of IL-6 in two models of fracture repair (full fracture and stress fracture) using 12-week old IL-6 global knockout mice (IL-6 KO) and wild type (WT) littermate controls. Read More

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February 2021

Guided Bone Regeneration with Ammoniomethacrylate-Based Barrier Membranes in a Radial Defect Model.

Biomed Res Int 2020 13;2020:5905740. Epub 2020 Oct 13.

Department of Orthopedic Surgery, Hadassah-Hebrew University Medical Center, Jerusalem 91120, Israel.

Large bone defects pose an unsolved challenge for orthopedic surgeons. Our group has previously reported the construction of a barrier membrane made of ammoniomethacrylate copolymer USP (AMCA), which supports the adhesion, proliferation, and osteoblastic differentiation of human mesenchymal stem cells (hMSCs). In this study, we report the use of AMCA membranes to seclude critical segmental defect (~1. Read More

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Mechanobiology of Bone Consolidation During Distraction Osteogenesis: Bone Lengthening Vs. Bone Transport.

Ann Biomed Eng 2021 Apr 27;49(4):1209-1221. Epub 2020 Oct 27.

Escuela Técnica Superior de Ingeniería, Universidad de Sevilla, Avenida Camino de los Descubrimientos s/n, 41092, Seville, Spain.

Bone lengthening and bone transport are regeneration processes that commonly rely on distraction osteogenesis, a widely accepted surgical procedure to deal with numerous bony pathologies. Despite the extensive study in the literature of the influence of biomechanical factors, a lack of knowledge about their mechanobiological differences prevents a clinical particularization. Bone lengthening treatments were performed on sheep metatarsus by reproducing the surgical and biomechanical protocol of previous bone transport experiments. Read More

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Heterogeneity of the osteocyte lacuno-canalicular network architecture and material characteristics across different tissue types in healing bone.

J Struct Biol 2020 11 11;212(2):107616. Epub 2020 Sep 11.

Max Planck Institute of Colloids and Interfaces, Department of Biomaterials, Am Mühlenberg 1, 14476 Potsdam, Germany. Electronic address:

Various tissue types, including fibrous connective tissue, bone marrow, cartilage, woven and lamellar bone, coexist in healing bone. Similar to most bone tissue type, healing bone contains a lacuno-canalicular network (LCN) housing osteocytes. These cells are known to orchestrate bone remodeling in healthy bone by sensing mechanical strains and translating them into biochemical signals. Read More

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November 2020

Genistein Improves Bone Healing via Triggering Estrogen Receptor Alpha-Mediated Expressions of Osteogenesis-Associated Genes and Consequent Maturation of Osteoblasts.

J Agric Food Chem 2020 Sep 8;68(39):10639-10650. Epub 2020 Sep 8.

Anesthesiology and Health Policy Research Center, Taipei Medical University, Taipei 11031, Taiwan.

Osteoporosis-associated fractures may cause higher morbidity and mortality. Our previous study showed the effects of genistein, a phytoestrogen, on the induction of estrogen receptor alpha (ERα) gene expression and stimulation of osteoblast mineralization. In this study, rat calvarial osteoblasts and an animal bone defect model were used to investigate the effects of genistein on bone healing. Read More

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September 2020

Effect of Selective Androgen Receptor Modulator Enobosarm on Bone Healing in a Rat Model for Aged Male Osteoporosis.

Calcif Tissue Int 2020 12 2;107(6):593-602. Epub 2020 Sep 2.

Department of Trauma Surgery, Orthopaedics and Plastic Surgery, University Medical Center Goettingen, Robert-Koch Str. 40, 37075, Goettingen, Germany.

Enobosarm (ostarine, MK-2866, or GTx-024) is a non-steroidal selective androgen receptor modulator. This study evaluated the effect of various regimens of enobosarm (EN) on bone healing in an orchiectomized rat model for aged male osteoporosis and compared it to testosterone (T) treatment. Ninety eight-month-old male Sprague Dawley rats were either orchiectomized (Orx) or left intact (Non-Orx) and divided into groups (n = 15/group): (1) Non-Orx; (2) Orx; (3) Orx+T-th; (4) Orx+EN-th; (5) Orx+T-pr; and (6) Orx+EN-pr. Read More

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December 2020

Differences in the microarchitectural features of the lateral collapsed lesion between osteonecrosis and subchondral insufficiency fracture of the femoral head.

Bone 2020 12 12;141:115585. Epub 2020 Aug 12.

Department of Orthopaedic Surgery, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Electronic address:

Background: Like osteonecrosis of the femoral head (ONFH), subchondral insufficiency fracture of the femoral head (SIF) causes femoral head collapse. However, little is known about the differences between the two diseases regarding the morphological features of the collapsed lesion. We tested the hypothesis that the morphological features of the lateral collapsed lesion would differ between ONFH and SIF. Read More

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December 2020

Assessment of bone healing using (Ti,Mg)N thin film coated plates and screws: Rabbit femur model.

J Biomed Mater Res B Appl Biomater 2021 02 7;109(2):227-237. Epub 2020 Aug 7.

Department of Biomedical Engineering, Yildiz Technical University, Istanbul, Turkey.

Magnesium (Mg) based implants such as plates and screws are often preferred to treat bone defects because of the positive effects of magnesium in bone growth and healing. Their low corrosion resistance, however, leads to fast degradation and consequently failure before healing was completed. Previously, we developed Mg doped titanium nitrate (TiN) thin film coatings to address these limitations and demonstrated that <10 at% Mg doping led to enhanced mineralization in vitro. Read More

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February 2021

Percutaneous CO2 Treatment Accelerates Bone Generation During Distraction Osteogenesis in Rabbits.

Clin Orthop Relat Res 2020 08;478(8):1922-1935

Y. Kumabe, T. Fukui, S. Takahara, Y. Kuroiwa, M. Arakura, K. Oe, T. Oda, K. Sawauchi, T. Matsushita, T. Matsumoto, S. Hayashi, R. Kuroda, T. Niikura, Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.

Background: Distraction osteogenesis has been broadly used to treat various structural bone deformities and defects. However, prolonged healing time remains a major problem. Various approaches including the use of low-intensity pulsed ultrasound, parathyroid hormone, and bone morphogenetic proteins (BMPs) have been studied to shorten the treatment period with limited success. Read More

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Gender differences in tibial fractures healing in normal and muscular dystrophic mice.

Am J Transl Res 2020 15;12(6):2640-2651. Epub 2020 Jun 15.

Department of Orthopaedic Surgery, McGovern Medical School, University of Texas Health Science Center at Houston Houston, TX 77054, USA.

Duchenne muscular dystrophy (DMD) patients have a high fracture risk and poor fracture healing. The dystrophin () mouse is a murine model of DMD and exhibits delayed bone fracture healing. Since our research team has shown that adult stem cells, such as muscle-derived stem cells, display a gender difference in their osteogenic potential with the male cells being more osteogenic, we hypothesize that a potential gender differences may exist during bone healing in normal and mice. Read More

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Effect of kirenol on the interaction between the WNT/β-Catenin and RUNX2/TCF/LEF1 pathways in fracture healing in vivo.

Acta Orthop Traumatol Turc 2020 May;54(3):320-329

Department of Orthopedics and Traumatology, Erciyes University, School of Medicine, Kayseri, Turkey.

Objective: This study aimed to determine the effects of a natural diterpenoid, kirenol, on fracture healing in vivo in an experimental rat model of femur fracture and investigate its potential mechanism of action via the Wnt/β-catenin pathway.

Methods: In this study, 64 male Wistar albino rats aged 5-7 weeks and weighing 261-348 g were randomly divided into 8 groups from A to L, with eight rats in each group. Standardized fractures were created in the right femurs of the rats and then fixed with an intramedullary Kirschner wire. Read More

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Osteoking downregulates Mgp in an osteoporotic fracture rat model.

J Tradit Chin Med 2020 06;40(3):422-431

Key Laboratory of Bio-active Peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, Kunming Institute of Zoology, Kunming Yunnan, 650032, China.

Objective: To investigate the effectiveness of osteoking, a Traditional Chinese Medicine originating from Yi nationality, against osteoporosis (OP) and osteoporotic fracture (OPF), and to elucidate its mechanism of action.

Methods: An osteoporotic fracture rat model was established; animals were divided into three treatment groups: parathyroid hormone, osteoking and 0.9%NaCl. Read More

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