24,247 results match your criteria calcium fluorescence

Building Your Own Neuroscience Equipment: A Precision Micromanipulator and an Epi-fluorescence Microscope for Calcium Imaging.

J Undergrad Neurosci Educ 2020 31;19(1):A134-A140. Epub 2020 Dec 31.

Department of Neurobiology and Behavior, Cornell University, Ithaca, NY 14853.

A faculty member's ability to develop meaningful research-oriented laboratories in neurobiology is often hampered by the rapid pace of new technologies and the increasing cost of equipment. To help undergraduate neuroscience faculty meet these challenges, we introduce two important neuroscience research tools we designed and built. The first is a precision micromanipulator for neurophysiology applications costing less than $40 USD. Read More

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December 2020

Single-cell visualization and quantification of trace metals in lysosome-related organelles.

Proc Natl Acad Sci U S A 2021 Apr;118(16)

California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720;

The acidocalcisome is an acidic organelle in the cytosol of eukaryotes, defined by its low pH and high calcium and polyphosphate content. It is visualized as an electron-dense object by transmission electron microscopy (TEM) or described with mass spectrometry (MS)-based imaging techniques or multimodal X-ray fluorescence microscopy (XFM) based on its unique elemental composition. Compared with MS-based imaging techniques, XFM offers the additional advantage of absolute quantification of trace metal content, since sectioning of the cell is not required and metabolic states can be preserved rapidly by either vitrification or chemical fixation. Read More

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Nimodipine inhibits intestinal and aortic smooth muscle contraction by regulating Ca-activated Cl channels.

Toxicol Appl Pharmacol 2021 Apr 16:115543. Epub 2021 Apr 16.

Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, School of Life Sciences, Liaoning Normal University, Dalian 116000, PR China. Electronic address:

Nimodipine is a clinically used dihydropyridine L-type calcium channel antagonist that effectively inhibits transmembrane Ca influx following the depolarization of smooth muscle cells, but the detailed effect on smooth muscle contraction is not fully understood. Ca-activated Cl channels (CaCCs) in vascular smooth muscle cells (VSMCs) may regulate vascular contractility. We found that nimodipine can inhibit transmembrane protein 16A (TMEM16A) activity in a concentration-dependent manner by cell-based fluorescence-quenching assay and short-circuit current analysis, with an IC value of ~5 μM. Read More

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3,3'-Diindolylmethane induces gastric cancer cells death via STIM1 mediated store-operated calcium entry.

Int J Biol Sci 2021 19;17(5):1217-1233. Epub 2021 Mar 19.

Department of Health Care, Zhenjiang Fourth Peoples Hospital, Zhenjiang, China.

3,3'-Diindolylmethane (DIM), a natural phytochemicals isolated from cruciferous vegetables, has been reported to inhibit human gastric cancer cells proliferation and induce cells apoptosis as well as autophagy, but its mechanisms are still unclear. Store-operated calcium entry (SOCE) is a main Ca influx pathway in various of cancers, which is activated by the depletion of endoplasmic reticulum (ER) Ca store. Stromal interaction molecular 1 (STIM1) is the necessary component of SOCE. Read More

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Serca2a-Phospholamban Interaction Monitored by an Interposed Circularly Permutated Green Fluorescent Protein.

Am J Physiol Heart Circ Physiol 2021 Apr 16. Epub 2021 Apr 16.

Lillehei Heart Institute, University of Minnesota College of Medicine, Department of Medicine, Minneapolis, MN 55455, United States.

Background: The interaction of phospholamban (PLB) and the sarcoplasmic reticulum Ca-ATPase (Serca2a) is a key regulator of cardiac contractility and a therapeutic target in heart failure (HF). PLB mediated increases in Serca2a activity improve cardiac function and HF. Clinically this mechanism can only be exploited by a general activation of the proteinkinase A (PKA) which is associated with side effects and adverse clinical outcomes. Read More

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Attenuated β-adrenergic response in calcium/calmodulin-dependent protein kinase IV-knockout mice.

PLoS One 2021 15;16(4):e0249932. Epub 2021 Apr 15.

Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Japan.

In the present study, we examined the importance of Ca2+/calmodulin-dependent protein kinase IV (CaMKIV) in the regulation of cardiac function using genetically modified CaMKIV-null mice. RT-PCR analysis revealed decreased expression of voltage-dependent calcium channels in the cardiac myocytes of CaMKIV-null mice compared with wild-type mice. CaMKIV-null mice showed shortened QT time on electrocardiograms. Read More

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In-situ datasets of important physical and bio-chemical parameters in the continental shelf of the northern Bay of Bengal.

Data Brief 2021 Apr 15;35:106947. Epub 2021 Mar 15.

Bangladesh Oceanographic Research Institute, Ramu, Cox's bazar, Bangladesh.

Data equipped with this article were collected from Northern Bay of Bengal (NBoB) wrapping both the eastern and western coast for CTD and sediment samples and only the eastern coast for water sampling. In-situ data of physical parameters, heavy metals, elements, Total Organic Carbon (TOC), nutrients, chlorophyll-a and phaeopigment were sampled across the shallow continental shelf. These data were assembled from 15 CTD points, 76 water samples, and 10 surface sediment samples adjacent to Bangladesh coast. Read More

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Nicotine induces morphological and functional changes in astrocytes via nicotinic receptor activity.

Glia 2021 Apr 14. Epub 2021 Apr 14.

Department of Chemistry, University of Kentucky, Lexington, Kentucky, USA.

Nicotine is a highly addictive compound present in tobacco, which causes the release of dopamine in different regions of the brain. Recent studies have shown that astrocytes express nicotinic acetylcholine receptors (nAChRs) and mediate calcium signaling. In this study, we examine the morphological and functional adaptations of astrocytes due to nicotine exposure. Read More

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Int J Pharm 2021 Apr 9:120588. Epub 2021 Apr 9.

Experimental Cancer Medicine, Clinical Research Center, Karolinska Institutet, Stockholm Sweden; Clinical Research Center (KFC) and Center for Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital Huddinge, Stockholm, Sweden. Electronic address:

Aim: The study was designed to evaluate the ability of the calcium sulfate based NanoZolid® drug delivery technology to locally release the epidermal growth factor (EGF) protein while maintaining its biological activity.

Methods: NanoZolid-formulated EGF protein labelled with a near infrared dye (EGF-NIR) depots or EGF-NIR dissolved in PBS were injected subcutaneously into mice bearing EGF receptor (EGFR) positive human A549 lung cancer tumors inoculated subcutaneously. The release and biodistribution of the EGF-NIR were investigated in vivo longitudinally up to 96 hours post administration, utilizing whole body fluorescence imaging. Read More

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[Levels of 25 hydroxy vitamin D of serum and broncho-alveolar lavage in patients with pulmonary tuberculosis].

Rev Chilena Infectol 2021 Feb;38(1):37-44

Departamento de Medicina Interna y Neumología, Universidad Surcolombiana, Neiva, Colombia.

Background: Alteration of vitamin D is a risk factor for tuberculosis (TB).

Aim: To evaluate the pulmonary and serum levels of 25-hydroxy vitamin D (25OHD) in patients with and without pulmonary TB.

Methods: Two-stage study: the first part was retrospective cross-sectional and the second prospective. Read More

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February 2021

Calcium Imaging Analysis of Cellular Responses to Hypercapnia and Hypoxia in the NTS of Newborn Rat Brainstem Preparation.

Front Physiol 2021 25;12:645904. Epub 2021 Mar 25.

Clinical Research Center, Murayama Medical Center, Musashimurayama, Japan.

It is supposed that the nucleus of the solitary tract (NTS) in the dorsal medulla includes gas sensor cells responsive to hypercapnia or hypoxia in the central nervous system. In the present study, we analyzed cellular responses to hypercapnia and hypoxia in the NTS region of newborn rat preparation. The brainstem and spinal cord were isolated from newborn rat (P0-P4) and were transversely cut at the level of the rostral area postrema. Read More

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IRE1α-XBP1 Affects the Mitochondrial Function of Aβ25-35-Treated SH-SY5Y Cells by Regulating Mitochondria-Associated Endoplasmic Reticulum Membranes.

Front Cell Neurosci 2021 25;15:614556. Epub 2021 Mar 25.

Department of Neurology, Second Hospital of Shandong University, Jinan, China.

Neurotoxicity induced by the amyloid beta (Aβ) peptide is one of the most important pathological mechanisms of Alzheimer's disease (AD). Activation of the adaptive IRE1α-XBP1 pathway contributes to the pathogenesis of AD, making it a potential target for AD therapeutics. However, the mechanism of IRE1α-XBP1 pathway involvement in AD is unclear. Read More

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Comparative Effects of Event Detection Methods on the Analysis and Interpretation of Ca Imaging Data.

Front Neurosci 2021 26;15:620869. Epub 2021 Mar 26.

Department of Neuroscience, School of Medicine, University of Kentucky, Lexington, KY, United States.

Calcium imaging has gained substantial popularity as a tool to profile the activity of multiple simultaneously active cells at high spatiotemporal resolution. Among the diverse approaches to processing of Ca imaging data is an often subjective decision of how to quantify baseline fluorescence or . We examine the effect of popular determination methods on the interpretation of neuronal and astrocyte activity in a single dataset of rats trained to self-administer intravenous infusions of cocaine and compare them with an -independent wavelet ridgewalking event detection approach. Read More

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Efficacy of short-synthetic antifungal peptides on pathogenic Aspergillus flavus.

Pestic Biochem Physiol 2021 May 27;174:104810. Epub 2021 Feb 27.

Department of Biochemistry, University College of Science, Osmania University, Hyderabad 500007, Telangana State, India. Electronic address:

The efficacies of three short synthetic antifungal peptides were tested for their inhibitory action on pathogenic fungi, Aspergillus flavus. The sequences of the short synthetic peptides are PPD1- FRLHF, 66-10-FRLKFH, 77-3- FRLKFHF, respectively. These test peptides inhibited fungal growth and showed a membranolytic activity. Read More

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Simultaneous removal of calcium, fluoride, nickel, and nitrate using microbial induced calcium precipitation in a biological immobilization reactor.

J Hazard Mater 2021 Mar 27;416:125776. Epub 2021 Mar 27.

School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.

In this research, an immobilized biofilm reactor was established for the simultaneous removal of calcium (Ca), fluoride (F), nickel (Ni), and nitrate (NO-N) by microbial induced calcium precipitation (MICP). The operating parameters of the reactor, hydraulic retention time (HRT: 4, 8, and 12 h), influent Ca concentration (36.0, 108. Read More

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Performance and mechanism of oxidation, and removal of trace SeO in flue gas utilizing a HO, NaClO, and Ca slurry.

Environ Sci Pollut Res Int 2021 Apr 8. Epub 2021 Apr 8.

Hebei Key Lab of Power Plant Flue Gas Multi-Pollutants Control, Department of Environmental Science and Engineering, North China Electric Power University, Baoding, 071003, People's Republic of China.

Selenium (Se) is one of the toxic trace elements in flue gas emitted from coal-fired power plants. A method for oxidation and absorption of selenium dioxide (SeO) by hydrogen peroxide (HO) and sodium chlorite (NaClO) in a calcium-based slurry is proposed, in which hydrated lime (Ca(OH)) and calcium carbonate (CaCO) were selected to be absorbents, and HO and NaClO were used to oxidize SeO and selenite ion to selenate ion to reduce their toxicities. The effects of reaction temperature, HO and NaClO concentrations, solution pH, and SeO concentration were investigated, from which the optimal reaction conditions were established. Read More

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A ratiometric fluorescent probe based on sulfur quantum dots and calcium ion for sensitive and visual detection of doxycycline in food.

Food Chem 2021 Mar 30;356:129720. Epub 2021 Mar 30.

School of Chemistry, Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, South China Normal University, Guangzhou 510006, China.

The residue of doxycycline in food can cause harm to human. Therefore, the detection of doxycycline residue is necessary. Herein, a ratiometric fluorescent probe was designed based on sulfur quantum dots (S dots) and Ca. Read More

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Magnesium and calcium over-accumulate in the leaves of a schengen3 mutant of Brassica rapa.

Plant Physiol 2021 Apr 8. Epub 2021 Apr 8.

School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.

Magnesium (Mg) and calcium (Ca) are essential mineral nutrients poorly supplied in many human food systems. In grazing livestock, Mg and Ca deficiencies are costly welfare issues. Here, we report a Brassica rapa loss-of-function schengen3 (sgn3) mutant, braA. Read More

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Miniaturized head-mounted microscope for whole-cortex mesoscale imaging in freely behaving mice.

Nat Methods 2021 Apr 5;18(4):417-425. Epub 2021 Apr 5.

Department of Biomedical Engineering, University of Minnesota, Twin Cities, Minneapolis, MN, USA.

The advent of genetically encoded calcium indicators, along with surgical preparations such as thinned skulls or refractive-index-matched skulls, has enabled mesoscale cortical activity imaging in head-fixed mice. However, neural activity during unrestrained behavior substantially differs from neural activity in head-fixed animals. For whole-cortex imaging in freely behaving mice, we present the 'mini-mScope', a widefield, miniaturized, head-mounted fluorescence microscope that is compatible with transparent polymer skull preparations. Read More

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The Tolerance of to Soil Contamination with Arsenic.

Plants (Basel) 2021 Mar 25;10(4). Epub 2021 Mar 25.

GeoBioTec, Departamento de Ciências da Terra, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Campus da Caparica, 2829-516 Caparica, Portugal.

The contamination of abandoned mining areas is a problem worldwide that needs urgent attention. Phytoremediation emerges as a successful method to extract different contaminants from the soil. In this context, plants growing in soils artificial contaminated with arsenic (As) were used to access its phytoremediation capabilities. Read More

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Cytotoxic Activity of LLO Y406A Is Targeted to the Plasma Membrane of Cancer Urothelial Cells.

Int J Mol Sci 2021 Mar 24;22(7). Epub 2021 Mar 24.

Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, SI-1000 Ljubljana, Slovenia.

Identification of novel agents for bladder cancer treatment is highly desirable due to the high incidence of tumor recurrence and the risk of progression to muscle-invasive disease. The key feature of the cholesterol-dependent toxin listeriolysin O mutant (LLO Y406A) is its preferential activity at pH 5.7, which could be exploited either directly for selective targeting of cancer cells or the release of accumulated therapeutics from acidic endosomes. Read More

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Effect of MgCl and GdCl on ORAI1 Expression and Store-Operated Ca Entry in Megakaryocytes.

Int J Mol Sci 2021 Mar 24;22(7). Epub 2021 Mar 24.

Department of Vegetative and Clinical Physiology, Eberhard Karls University, 72074 Tübingen, Germany.

In chronic kidney disease, hyperphosphatemia upregulates the Ca channel ORAI and its activating Ca sensor STIM in megakaryocytes and platelets. ORAI1 and STIM1 accomplish store-operated Ca entry (SOCE) and play a key role in platelet activation. Signaling linking phosphate to upregulation of ORAI1 and STIM1 includes transcription factor NFAT5 and serum and glucocorticoid-inducible kinase SGK1. Read More

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Epitope Binning of Novel Monoclonal Anti F1 and Anti LcrV Antibodies and Their Application in a Simple, Short, HTRF Test for Clinical Plague Detection.

Pathogens 2021 Mar 2;10(3). Epub 2021 Mar 2.

The Department of Infectious Diseases, Israel Institute for Biological Research, Ness-Ziona 7410001, Israel.

Mouse monoclonal antibodies were raised against plague disease biomarkers: the bacterial capsular protein fraction 1 (F1) and the low-calcium response-LcrV virulence factor (Vag). A novel tandem assay, employing BioLayer Interferometry (BLI), enabled the isolation of antibodies against four different epitopes on Vag. The tandem assay was carried out with hybridoma supernatants, circumventing the need for antibody purification. Read More

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The HaloTag as a general scaffold for far-red tunable chemigenetic indicators.

Nat Chem Biol 2021 Apr 1. Epub 2021 Apr 1.

Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.

Functional imaging using fluorescent indicators has revolutionized biology, but additional sensor scaffolds are needed to access properties such as bright, far-red emission. Here, we introduce a new platform for 'chemigenetic' fluorescent indicators, utilizing the self-labeling HaloTag protein conjugated to environmentally sensitive synthetic fluorophores. We solve a crystal structure of HaloTag bound to a rhodamine dye ligand to guide engineering efforts to modulate the dye environment. Read More

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Calcium-Sensing Receptor Contributes to Hyperoxia Effects on Human Fetal Airway Smooth Muscle.

Front Physiol 2021 15;12:585895. Epub 2021 Mar 15.

Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, Rochester, MN, United States.

Supplemental O (hyperoxia), necessary for maintenance of oxygenation in premature infants, contributes to neonatal and pediatric airway diseases including asthma. Airway smooth muscle (ASM) is a key resident cell type, responding to hyperoxia with increased contractility and remodeling [proliferation, extracellular matrix (ECM) production], making the mechanisms underlying hyperoxia effects on ASM significant. Recognizing that fetal lungs experience a higher extracellular Ca ([Ca]) environment, we previously reported that the calcium sensing receptor (CaSR) is expressed and functional in human fetal ASM (fASM). Read More

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Simultaneous readout of multiple FRET pairs using photochromism.

Nat Commun 2021 03 31;12(1):2005. Epub 2021 Mar 31.

Department of Chemistry, KU Leuven, Leuven, Belgium.

Förster resonant energy transfer (FRET) is a powerful mechanism to probe associations in situ. Simultaneously performing more than one FRET measurement can be challenging due to the spectral bandwidth required for the donor and acceptor fluorophores. We present an approach to distinguish overlapping FRET pairs based on the photochromism of the donor fluorophores, even if the involved fluorophores display essentially identical absorption and emission spectra. Read More

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TRPV4 - Rho signaling drives cytoskeletal and focal adhesion remodeling in trabecular meshwork cells.

Am J Physiol Cell Physiol 2021 Mar 31. Epub 2021 Mar 31.

Departments of Ophthalmology & Visual Sciences; Bioengineering; Neurobiology & Anatomy, University of Utah, Salt Lake City, UT, United States.

Intraocular pressure (IOP) is dynamically regulated by the trabecular meshwork (TM), a mechanosensitive tissue that protects the eye from injury through dynamic regulation of aqueous humor outflow from the anterior chamber of the eye. In response to chronic IOP elevations, TM compensates for mechanical stress through increased actin polymerization, tissue stiffness and contractility. This process has been associated with open angle glaucoma, however, the mechanisms that link mechanical stress to pathological cytoskeletal remodeling downstream from the mechanotransducers remain poorly understood. Read More

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Costunolide attenuates oxygen‑glucose deprivation/reperfusion‑induced mitochondrial‑mediated apoptosis in PC12 cells.

Mol Med Rep 2021 Jun 31;23(6). Epub 2021 Mar 31.

Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China.

The present study investigated the effect of costunolide (CT), a compound extracted from A Decne, to attenuate oxygen‑glucose deprivation/reperfusion (OGD/R)‑induced mitochondrial‑mediated apoptosis in PC12 cells. The present study used molecular docking technology to detect the binding of CT with mitochondrial apoptotic protein targets. A model of oxygen‑glucose deprivation for 2 h and reperfusion for 24 h in PC12 cells was used to mimic cerebral ischemic injury. Read More

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Functional characterization of N-octyl 4-methylamphetamine variants and related bivalent compounds at the dopamine and serotonin transporters using Ca channels as sensors.

Toxicol Appl Pharmacol 2021 May 27;419:115513. Epub 2021 Mar 27.

Department of Physiology and Biophysics, School of Medicine, Virginia Commonwealth University, United States of America. Electronic address:

The early characterization of ligands at the dopamine and serotonin transporters, DAT and SERT, respectively, is important for drug discovery, forensic sciences, and drug abuse research. 4-Methyl amphetamine (4-MA) is a good example of an abused drug whose overdose can be fatal. It is a potent substrate at DAT and SERT where its simplest secondary amine (N-methyl 4-MA) retains substrate activity at them. Read More

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Anti-arrhythmic and inotropic effects of empagliflozin following myocardial ischemia.

Life Sci 2021 Mar 27;276:119440. Epub 2021 Mar 27.

The Hull Family Cardiac Fibrillation Management Laboratory, Toronto General Hospital, Canada. Electronic address:

Background: Empagliflozin (EMPA) reduces heart failure hospitalization and mortality. The benefit in terms of ventricular arrhythmia and contractility has not been explored.

Objective: To determine the direct effects of EMPA on ventricular arrhythmia and cardiac contractility in an ex-vivo model of global ischemia-reperfusion (I/R). Read More

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