17,152 results match your criteria ca2+ signal

Inhibition of Shear-Induced Platelet Aggregation by Xueshuantong via Targeting Piezo1 Channel-Mediated Ca Signaling Pathway.

Front Pharmacol 2021 22;12:606245. Epub 2021 Mar 22.

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.

XueShuanTong (XST) comprising therapeutically active ginsenosides, a lyophilized extract of roots, is extensively used in traditional Chinese medicine to treat ischemic heart and cerebrovascular diseases. Our recent study shows that treatment with XST inhibits shear-induced thrombosis formation but the underlying mechanism remained unclear. This study aimed to investigate the hypothesis that XST inhibited shear-induced platelet aggregation via targeting the mechanosensitive Ca-permeable Piezo1 channel by performing platelet aggregation assay, Ca imaging and Western blotting analysis. Read More

View Article and Full-Text PDF

Regulation of the tubulin polymerization-promoting protein by Ca/S100 proteins.

Cell Calcium 2021 Mar 27;96:102404. Epub 2021 Mar 27.

Applied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama 700-8530, Japan. Electronic address:

To elucidate S100 protein-mediated signaling pathways, we attempted to identify novel binding partners for S100A2 by screening protein arrays carrying 19,676 recombinant glutathione S-transferase (GST)-fused human proteins with biotinylated S100A2. Among newly discovered putative S100A2 interactants, including TMLHE, TRH, RPL36, MRPS34, CDR2L, OIP5, and MED29, we identified and characterized the tubulin polymerization-promoting protein (TPPP) as a novel S100A2-binding protein. We confirmed the interaction of TPPP with Ca/S100A2 by multiple independent methods, including the protein array method, S100A2 overlay, and pulldown assay in vitro and in transfected COS-7 cells. Read More

View Article and Full-Text PDF

Dual-Reporting Transcriptionally Linked Genetically Encoded Fluorescent Indicators Resolve the Spatiotemporal Coordination of Cytosolic Abscisic Acid and Second Messenger Dynamics in Arabidopsis.

Plant Cell 2020 Aug;32(8):2582-2601

Entwicklungsbiologie der Pflanzen, Centre for Organismal Studies, Ruprecht-Karls-Universität Heidelberg, 69120 Heidelberg, Germany.

Deciphering signal transduction processes is crucial for understanding how plants sense and respond to environmental changes. Various chemical compounds function as central messengers within deeply intertwined signaling networks. How such compounds act in concert remains to be elucidated. Read More

View Article and Full-Text PDF

Possible effects of chemokine-like factor-like MARVEL transmembrane domain-containing family on antiphospholipid syndrome.

Chin Med J (Engl) 2021 Apr 1. Epub 2021 Apr 1.

Department of Rheumatology and Immunology, Peking University Third Hospital, Beijing 100191, China.

Abstract: Antiphospholipid syndrome (APS) is a systemic autoimmune disease defined by thrombotic or obstetrical events and persistent antiphospholipid antibodies (aPLs). Chemokine-like factor-like MARVEL transmembrane domain-containing family (CMTM) is widely expressed in the immune system and may closely related to APS. This review aimed to systematically summarize the possible effects of CMTM on APS. Read More

View Article and Full-Text PDF

Ryanodine receptor-mediated Ca release and atlastin-2 GTPase activity contribute to IP-induced dendritic Ca signals in primary hippocampal neurons.

Cell Calcium 2021 Mar 23;96:102399. Epub 2021 Mar 23.

Biomedical Neuroscience Institute, Universidad de Chile, Independencia 1027, Santiago, 8380453, Chile; Programa de Fisiología y Biofísica, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile; Department of Neurosciences and Center for Exercise, Metabolism and Cancer Studies, Facultad de Medicina, Universidad de Chile, Santiago, Chile. Electronic address:

Neuronal Ca signals are fundamental for synaptic transmission and activity-dependent changes in gene expression. Voltage-gated Ca channels and N-methyl-d-aspartate receptors play major roles in mediating external Ca entry during action potential firing and glutamatergic activity. Additionally, the inositol-1,4,5-trisphosphate receptor (IPR) and the ryanodine receptor (RyR) channels expressed in the endoplasmic reticulum (ER) also contribute to the generation of Ca signals in response to neuronal activity. Read More

View Article and Full-Text PDF

The MAMs Structure and Its Role in Cell Death.

Cells 2021 Mar 16;10(3). Epub 2021 Mar 16.

China Japan Union Hospital, Jilin University, Changchun 130031, China.

The maintenance of cellular homeostasis involves the participation of multiple organelles. These organelles are associated in space and time, and either cooperate or antagonize each other with regards to cell function. Crosstalk between organelles has become a significant topic in research over recent decades. Read More

View Article and Full-Text PDF

Identification and Biochemical Characterization of High Mobility Group Protein 20A as a Novel Ca/S100A6 Target.

Biomolecules 2021 Mar 30;11(4). Epub 2021 Mar 30.

Applied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama 700-8530, Japan.

During screening of protein-protein interactions, using human protein arrays carrying 19,676 recombinant glutathione s-transferase (GST)-fused human proteins, we identified the high-mobility protein group 20A (HMG20A) as a novel S100A6 binding partner. We confirmed the Ca-dependent interaction of HMG20A with S100A6 by the protein array method, biotinylated S100A6 overlay, and GST-pulldown assay in vitro and in transfected COS-7 cells. Co-immunoprecipitation of S100A6 with HMG20A from HeLa cells in a Ca-dependent manner revealed the physiological relevance of the S100A6/HMG20A interaction. Read More

View Article and Full-Text PDF

Regulation of Neutrophil Functions by Hv1/VSOP Voltage-Gated Proton Channels.

Int J Mol Sci 2021 Mar 5;22(5). Epub 2021 Mar 5.

Integrative Physiology, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita 5650871, Osaka, Japan.

The voltage-gated proton channel, Hv1, also termed VSOP, was discovered in 2006. It has long been suggested that proton transport through voltage-gated proton channels regulate reactive oxygen species (ROS) production in phagocytes by counteracting the charge imbalance caused by the activation of NADPH oxidase. Discovery of Hv1/VSOP not only confirmed this process in phagocytes, but also led to the elucidation of novel functions in phagocytes. Read More

View Article and Full-Text PDF

Ca Signalling Induced by NGF Identifies a Subset of Capsaicin-Excitable Neurons Displaying Enhanced Chemo-Nociception in Dorsal Root Ganglion Explants from Adult Mouse.

Int J Mol Sci 2021 Mar 4;22(5). Epub 2021 Mar 4.

International Centre for Neurotherapeutics, Dublin City University, Collins Avenue, D09 V209 Dublin, Ireland.

Nociceptors sense hazards via plasmalemmal cation channels, including transient receptor potential vanilloid 1 (TRPV1). Nerve growth factor (NGF) sensitises TRPV1 to capsaicin (CAPS), modulates nociceptor excitability and induces thermal hyperalgesia, but cellular mechanisms remain unclear. Confocal microscopy was used to image changes in intracellular Ca concentration ([Ca]) across neuronal populations in dorsal root ganglia (DRG) explants from adult mice, which express a fluorescent reporter in their sensory neurons. Read More

View Article and Full-Text PDF

Neuroprotective Effect of Gallocatechin Gallate on Glutamate-Induced Oxidative Stress in Hippocampal HT22 Cells.

Molecules 2021 Mar 4;26(5). Epub 2021 Mar 4.

College of Korean Medicine, Gachon University, Seongnam 13120, Korea.

Oxidative stress leads to protein degeneration or mitochondrial dysfunction, causing neuronal cell death. Glutamate is a neurotransmitter that nerve cells use to send signals. However, the excess accumulation of glutamate can cause excitotoxicity in the central nervous system. Read More

View Article and Full-Text PDF

Oxytocin Downregulates the Ca1.2 L-Type Ca Channel via Gi/cAMP/PKA/CREB Signaling Pathway in Cardiomyocytes.

Membranes (Basel) 2021 Mar 25;11(4). Epub 2021 Mar 25.

Department of Pathophysiology, Oita University School of Medicine, Oita 879-5593, Japan.

Oxytocin (OT) and its receptor (OTR) are expressed in the heart and are involved in the physiological cardiovascular functional system. Although it is known that OT/OTR signaling is cardioprotective by reducing the inflammatory response and improving cardiovascular function, the role of OT in the cardiac electrical excitation modulation has not been clarified. This study investigates the molecular mechanism of the action of OT on cardiomyocyte membrane excitation focusing on the L-type Ca channel. Read More

View Article and Full-Text PDF

The Role of CaMKII and ERK Signaling in Addiction.

Int J Mol Sci 2021 Mar 20;22(6). Epub 2021 Mar 20.

Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan.

Nicotine is the predominant addictive compound of tobacco and causes the acquisition of dependence through its interactions with nicotinic acetylcholine receptors and various neurotransmitter releases in the central nervous system. The Ca/calmodulin-dependent protein kinase II (CaMKII) and extracellular signal-regulated kinase (ERK) play a pivotal role in synaptic plasticity in the hippocampus. CaMKII is involved in long-term potentiation induction, which underlies the consolidation of learning and memory; however, the roles of CaMKII in nicotine and other psychostimulant-induced addiction still require further investigation. Read More

View Article and Full-Text PDF

Crosstalk among Calcium ATPases: PMCA, SERCA and SPCA in Mental Diseases.

Int J Mol Sci 2021 Mar 10;22(6). Epub 2021 Mar 10.

Department of Molecular Neurochemistry, Medical University of Lodz, 92215 Lodz, Poland.

Calcium in mammalian neurons is essential for developmental processes, neurotransmitter release, apoptosis, and signal transduction. Incorrectly processed Ca signal is well-known to trigger a cascade of events leading to altered response to variety of stimuli and persistent accumulation of pathological changes at the molecular level. To counterbalance potentially detrimental consequences of Ca, neurons are equipped with sophisticated mechanisms that function to keep its concentration in a tightly regulated range. Read More

View Article and Full-Text PDF

Long-term tracking and dynamically quantifying of reversible changes of extracellular Ca2+ in multiple brain regions of freely moving animals.

Angew Chem Int Ed Engl 2021 Apr 1. Epub 2021 Apr 1.

East China Normal University, Dept. of Chemistry, Dongchuan Road 500, 200062, Shanghai, CHINA.

Understanding physiological and pathological processes in the brain requires tracking the reversible changes in chemical signals with long-term stability. We developed a new anti-biofouling microfiber array to real-time quantify extracellular Ca 2+ concentrations together with neuron activity across many regions in the mammalian brain for 60 days, in which the signal degradation was < ∼8%. The developed microarray with high tempo-spatial resolution (∼10 μm, ∼1. Read More

View Article and Full-Text PDF

Ca entry at the plasma membrane and uptake by acidic stores is regulated by the activity of the V-H -ATPase in Toxoplasma gondii.

Mol Microbiol 2021 Apr 1. Epub 2021 Apr 1.

Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, GA, 30602-7400, USA.

Ca is a universal intracellular signal that regulates many cellular functions. In Toxoplasma gondii, the controlled influx of extracellular and intracellular Ca into the cytosol initiates a signaling cascade that promotes pathogenic processes like tissue destruction and dissemination. In this work we studied the role of proton transport in cytosolic Ca homeostasis and the initiation of Ca signaling. Read More

View Article and Full-Text PDF

Synergistic Effect and Mechanism of Apoptosis Induction by Morphine and the HIV-1gp120V3 Loop in Hippocampal Neurons.

J Neuroimmune Pharmacol 2021 Mar 31. Epub 2021 Mar 31.

Department of Pathophysiology, Key Laboratory of the State Administration of Traditional Chinese Medicine, school of Basic medical science and Public health, Jinan University, Guangzhou, 510632, Guangdong Province, China.

HIV-associated neurocognitive disorders (HAND) are a collective name for neurological disorders associated with HIV-1 infection. The incidence and severity of HAND are increased by concomitant opioid use disorder, such as heroin and morphine abuse. Our previous study showed that the HIV-1 envelope protein gp120 and morphine synergistically induce apoptosis in rat hippocampal neurons. Read More

View Article and Full-Text PDF

Interactive Roles of CaMKII/Ryanodine Receptor Signaling and Inflammation in Lung Diseases.

Adv Exp Med Biol 2021 ;1303:305-317

Department of Molecular and Cellular Physiology, Albany Medical College, Albany, NY, USA.

Ca/calmodulin-dependent protein kinase II (CaMKII) is a multifunctional protein kinase and has been recently recognized to play a vital role in pathological events in the pulmonary system. CaMKII has diverse downstream targets that promote vascular disease, asthma, and cancer, so improved understanding of CaMKII signaling has the potential to lead to new therapies for lung diseases. Multiple studies have demonstrated that CaMKII is involved in redox modulation of ryanodine receptors (RyRs). Read More

View Article and Full-Text PDF

Role of Airway Smooth Muscle in Inflammation Related to Asthma and COPD.

Hiroaki Kume

Adv Exp Med Biol 2021 ;1303:139-172

Department of Infectious Diseases and Respiratory Medicine, Fukushima Medical University Aizu Medical Center, Aizuwakamatsu, Japan.

Airway smooth muscle contributes to both contractility and inflammation in the pathophysiology of asthma and COPD. Airway smooth muscle cells can change the degree of a variety of functions, including contraction, proliferation, migration, and the secretion of inflammatory mediators (phenotype plasticity). Airflow limitation, airway hyperresponsiveness, β-adrenergic desensitization, and airway remodeling, which are fundamental characteristic features of these diseases, are caused by phenotype changes in airway smooth muscle cells. Read More

View Article and Full-Text PDF

Inositol 1,4,5-trisphosphate 3-kinase B promotes Ca mobilization and the inflammatory activity of dendritic cells.

Sci Signal 2021 Mar 30;14(676). Epub 2021 Mar 30.

Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.

Innate immune responses to Gram-negative bacteria depend on the recognition of lipopolysaccharide (LPS) by a receptor complex that includes CD14 and TLR4. In dendritic cells (DCs), CD14 enhances the activation not only of TLR4 but also that of the NFAT family of transcription factors, which suppresses cell survival and promotes the production of inflammatory mediators. NFAT activation requires Ca mobilization. Read More

View Article and Full-Text PDF

Transcriptional regulation of intermolecular Ca signaling in hibernating ground squirrel cardiomyocytes: The myocardin-junctophilin axis.

Proc Natl Acad Sci U S A 2021 Apr;118(14)

State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing 100871, China;

The contraction of heart cells is controlled by the intermolecular signaling between L-type Ca channels (LCCs) and ryanodine receptors (RyRs), and the nanodistance between them depends on the interaction between junctophilin-2 (JPH2) in the sarcoplasmic reticulum (SR) and caveolin-3 (CAV3) in the transversal tubule (TT). In heart failure, decreased expression of JPH2 compromises LCC-RyR communication leading to deficient blood-pumping power. In the present study, we found that JPH2 and CAV3 transcription was concurrently regulated by serum response factor (SRF) and myocardin. Read More

View Article and Full-Text PDF

Crocin Improves Endothelial Mitochondrial Dysfunction GPx1/ROS/KCa3.1 Signal Axis in Diabetes.

Front Cell Dev Biol 2021 12;9:651434. Epub 2021 Mar 12.

Department of Anatomy, Harbin Medical University, Harbin, China.

Mitochondrial dysfunction contributes to excessive reactive oxygen species (ROS) generation, which is a dramatic cause to promote endothelial dysfunction in diabetes. It was previously demonstrated that crocin protected the endothelium based on its diverse medicinal properties, but its effect on the mitochondrion and the potential mechanism are not fully understood. In this study, mitochondrial function was analyzed during the process of excessive ROS generation in high glucose (HG)-cultured human umbilical vein endothelial cells (HUVECs). Read More

View Article and Full-Text PDF

HN1L/JPT2: A signaling protein that connects NAADP generation to Ca microdomain formation.

Sci Signal 2021 Mar 23;14(675). Epub 2021 Mar 23.

The Ca Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.

NAADP-evoked Ca release through type 1 ryanodine receptors (RYR1) is a major mechanism underlying the earliest signals in T cell activation, which are the formation of Ca microdomains. In our characterization of the molecular machinery underlying NAADP action, we identified an NAADP-binding protein, called hematological and neurological expressed 1-like protein (HN1L) [also known as Jupiter microtubule-associated homolog 2 (JPT2)]. Gene deletion of in human Jurkat and primary rat T cells resulted in decreased numbers of initial Ca microdomains and delayed the onset and decreased the amplitude of global Ca signaling. Read More

View Article and Full-Text PDF

Essential requirement for JPT2 in NAADP-evoked Ca signaling.

Sci Signal 2021 03 23;14(675). Epub 2021 Mar 23.

Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.

Nicotinic acid adenine dinucleotide phosphate (NAADP) is a second messenger that releases Ca from acidic organelles through the activation of two-pore channels (TPCs) to regulate endolysosomal trafficking events. NAADP action is mediated by NAADP-binding protein(s) of unknown identity that confer NAADP sensitivity to TPCs. Here, we used a "clickable" NAADP-based photoprobe to isolate human NAADP-binding proteins and identified Jupiter microtubule-associated homolog 2 (JPT2) as a TPC accessory protein required for endogenous NAADP-evoked Ca signaling. Read More

View Article and Full-Text PDF

Insulinotropic and antidiabetic properties of Eucalyptus citriodora leaves and isolation of bioactive phytomolecules.

J Pharm Pharmacol 2021 Mar 23. Epub 2021 Mar 23.

School of Biomedical Sciences, Ulster University, Coleraine, Northern Ireland, UK.

Objective: The aim of this study was to delineate the mechanisms of action of the plant Eucalyptus citriodora used traditionally for the treatment of type 2 diabetes.

Methods: Insulin secretion and signal transduction were measured using clonal pancreatic β-cells and mouse islets. Glucose uptake was assessed using 3T3-L1 adipocytes and in vitro systems assessed additional glucose-lowering actions. Read More

View Article and Full-Text PDF

Spontaneous Ca Fluctuations Arise in Thin Astrocytic Processes With Real 3D Geometry.

Front Cell Neurosci 2021 1;15:617989. Epub 2021 Mar 1.

Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences (MTA), Budapest, Hungary.

Fluctuations of cytosolic Ca concentration in astrocytes are regarded as a critical non-neuronal signal to regulate neuronal functions. Although such fluctuations can be evoked by neuronal activity, rhythmic astrocytic Ca oscillations may also spontaneously arise. Experimental studies hint that these spontaneous astrocytic Ca oscillations may lie behind different kinds of emerging neuronal synchronized activities, like epileptogenic bursts or slow-wave rhythms. Read More

View Article and Full-Text PDF

Gastrodin ameliorates learning and memory impairment in rats with vascular dementia by promoting autophagy flux via inhibition of the Ca/CaMKII signal pathway.

Aging (Albany NY) 2021 Mar 10;13. Epub 2021 Mar 10.

The High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province and The High Educational Key Laboratory of Guizhou Province for Natural Medicinal Pharmacology and Druggability, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550025, P.R. China.

Vascular dementia (VD) is a common disease that occurs during human aging. Gastrodin (GAS) has potential benefits for the prevention and treatment of VD. In the present study, we investigated the effects of GAS on cognitive dysfunction in rats with VD induced by permanent middle cerebral artery occlusion (pMCAO) and explored the underlying mechanism. Read More

View Article and Full-Text PDF

Catalyzing Initial Responses to Environmental Stresses.

Trends Plant Sci 2021 Mar 8. Epub 2021 Mar 8.

Department of Chemistry, Seoul National University, Seoul 08826, Korea; Plant Genomics and Breeding Institute, Seoul National University, Seoul 08826, Korea. Electronic address:

Plants have evolved stress-sensing machineries that initiate rapid adaptive environmental stress responses. Cytosolic calcium ion (Ca) is the most prominent second messenger that couples extracellular signals with specific intracellular responses. Essential early events that generate a cytosolic Ca spike in response to environmental stress are starting to emerge. Read More

View Article and Full-Text PDF