24,639 results match your criteria c-type lectin


A novel C-type lectin from Crassostrea gigas involved in the innate defense against Vibrio alginolyticus.

Biochem Biophys Res Commun 2021 Jun 11;566:155-163. Epub 2021 Jun 11.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Ocean College, Beibu Gulf University, Qinzhou, 535011, China; College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China. Electronic address:

C-type lectins (CTLs) are important immune molecules that participate in invertebrate defense response. In the present work, a novel structural CTL (CgLec-4E) was identified from Crassostrea gigas, which encodes 237 amino acids (aa) with an extra long chain of aa and in the C-type CRD domain with EPA, QPG and WHD mutated motifs respectively. rCgLec-4E could agglutinate and inhibit the growth of Vibrio alginolyticus, except Chlorella, which might be relevant to three mutated motifs. Read More

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MelLec Exacerbates the Pathogenesis of -Induced Allergic Inflammation in Mice.

Front Immunol 2021 28;12:675702. Epub 2021 May 28.

Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom.

Environmental factors, particularly fungi, influence the pathogenesis of allergic airway inflammation, but the mechanisms underlying these effects are still unclear. Melanin is one fungal component which is thought to modulate pulmonary inflammation. We recently identified a novel C-type lectin receptor, MelLec (Clec1a), which recognizes fungal 1,8-dihydroxynaphthalene (DHN)-melanin and is able to regulate inflammatory responses. Read More

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C-type lectins and extracellular vesicles in virus-induced NETosis.

J Biomed Sci 2021 Jun 11;28(1):46. Epub 2021 Jun 11.

Genomics Research Center, Academia Sinica, 128, Academia Road, Sec. 2, Nankang District, Taipei, 115, Taiwan.

Dysregulated formation of neutrophil extracellular traps (NETs) is observed in acute viral infections. Moreover, NETs contribute to the pathogenesis of acute viral infections, including those caused by the dengue virus (DV) and severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Furthermore, excessive NET formation (NETosis) is associated with disease severity in patients suffering from SARS-CoV-2-induced multiple organ injuries. Read More

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Distinct antigen uptake receptors route to the same storage compartments for cross-presentation in dendritic cells.

Immunology 2021 Jun 10. Epub 2021 Jun 10.

Department of Immunology, Leiden University Medical Center, Postbus, 9600, 2300 RC, Leiden, The Netherlands.

An exclusive feature of dendritic cells (DCs) is their capacity to present exogenous antigens by MHC class I molecules, called cross-presentation. Here we show that protein antigen can be conserved in mature murine DCs for several days in a lysosome-like storage compartment, distinct from MHC class II and early endosomal compartments, as an internal source for the supply of MHC class I ligands. Using two different uptake routes via Fcγ receptors and C-type lectin receptors, we could show that antigens were routed towards the same endolysosomal compartments after 48 hours. Read More

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Chitinase 3-like-1 contributes to acetaminophen-induced liver injury by promoting hepatic platelet recruitment.

Elife 2021 Jun 10;10. Epub 2021 Jun 10.

Department of Anesthesiology, UTHealth McGovern Medical School, Houston, United States.

Hepatic platelet accumulation contributes to acetaminophen (APAP)-induced liver injury (AILI). However, little is known about the molecular pathways involved in platelet recruitment to the liver and whether targeting such pathways could attenuate AILI.

Mice were fasted overnight before injected with APAP at a dose of 210 mg/kg for male mice and 325 mg/kg for female mice. Read More

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CLEC14A protects against podocyte injury in mice with adriamycin nephropathy.

FASEB J 2021 Jul;35(7):e21711

Department of Pharmacology, School of Basic Medical Sciences, Shandong University, Jinan, China.

Podocyte injury is a major determinant of focal segmental glomerular sclerosis (FSGS) and the identification of potential therapeutic targets for preventing podocyte injury has clinical importance for the treatment of FSGS. CLEC14A is a single-pass transmembrane glycoprotein belonging to the vascular expressed C-type lectin family. CLEC14A is found to be expressed in vascular endothelial cells during embryogenesis and is also implicated in tumor angiogenesis. Read More

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Identification of Tumor Necrosis Factor-Alpha (TNF-α) Inhibitor in Rheumatoid Arthritis Using Network Pharmacology and Molecular Docking.

Front Pharmacol 2021 21;12:690118. Epub 2021 May 21.

The Fourth Affiliated Hospital, Anhui Medical University, Hefei, China.

This study aimed to investigate the molecular mechanism of Paeoniae Alba (white peony, WP) in treating immune inflammatory diseases of rheumatoid arthritis (RA) and tumor necrosis factor-alpha (TNF-α) inhibitors (TNFis) by using network pharmacology and molecular docking. In this study, the ingredient of WP and the potential inflammatory targets of RA were obtained from the Traditional Chinese Medicine Systematic Pharmacology Database, GeneCard, and OMIM databases, respectively. The establishment of the RA-WP-potential inflammatory target gene interaction network was accomplished using the STRING database. Read More

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Tumor cells express pauci- and oligomannosidic N-glycans in glycoproteins recognized by the mannose receptor (CD206).

Cell Mol Life Sci 2021 Jun 5. Epub 2021 Jun 5.

Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, CLS 11087 - 3 Blackfan Circle, Boston, MA, 02115, USA.

The macrophage mannose receptor (CD206, MR) is an endocytic lectin receptor which plays an important role in homeostasis and innate immunity, however, the endogenous glycan and glycoprotein ligands recognized by its C-type lectin domains (CTLD) have not been well studied. Here we used the murine MR CTLD4-7 coupled to the Fc-portion of human IgG (MR-Fc) to investigate the MR glycan and glycoprotein recognition. We probed 16 different cancer and control tissues using the MR-Fc, and observed cell- and tissue-specific binding with varying intensity. Read More

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A holistic QBD approach to design galactose conjugated PLGA polymer and nanoparticles to catch macrophages during intestinal inflammation.

Mater Sci Eng C Mater Biol Appl 2021 Jul 14;126:112183. Epub 2021 May 14.

Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.

Recruited macrophages in inflammation attract various ligand-receptor drug delivery approaches. Galactose bound nanocarriers are promising to catch macrophages because of surface-expressed macrophage galactose type-lectin-C (MGL-2) receptor. The present study reported fabrication of galactose conjugated PLGA (GAL-PLGA) polymer and nanoparticles under quality by design (QBD) approach to investigate macrophages targeting potential at inflamed intestine. Read More

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Oxidized low-density lipoprotein stimulates dendritic cells maturation via LOX-1-mediated MAPK/NF-κB pathway.

Braz J Med Biol Res 2021 31;54(9):e11062. Epub 2021 May 31.

Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, P.R. China.

Dendritic cells (DCs) play a crucial role as central orchestrators of immune system response in atherosclerosis initiation and progression. Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is involved in the immune maturation of DCs, but the underlying mechanisms remain unclear. We isolated mouse bone marrow progenitors and stimulated them with granulocyte-macrophage colony-stimulating factor and interleukin (IL)-4 to induce immature DCs. Read More

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Reviving lost binding sites: Exploring calcium binding-site transitions between human and murine CD23.

FEBS Open Bio 2021 Jun 2. Epub 2021 Jun 2.

King's College London, Randall Centre for Cell & Molecular Biophysics, London, SE1 1UL, United Kingdom.

Immunoglobulin E (IgE) is a central regulatory and triggering molecule of allergic immune responses.IgE's interaction with CD23 modulates both IgE production and functional activities.CD23 is a non-canonical immunoglobulin receptor, unrelated to receptors of other antibody isotypes. Read More

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Can Plant Lectins Help to Elucidate Insect Lectin-Mediated Immune Response?

Insects 2021 May 27;12(6). Epub 2021 May 27.

Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.

Lectins are carbohydrate-binding proteins that recognize and selectively bind to specific sugar structures. This group of proteins is widespread in plants, animals, and microorganisms, and exerts a broad range of functions. Many plant lectins were identified as exogenous stimuli of vertebrate immunity. Read More

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A C-Type Lectin Highly Expressed in Intestine Functions in AMP Regulation and Prophenoloxidase Activation.

Antibiotics (Basel) 2021 May 7;10(5). Epub 2021 May 7.

School of Marine Science, Ningbo University, Ningbo 315211, China.

A C-type lectin (PtCLec2) from was identified for characterization of its role in defense and innate immunity. PtCLec2 contains a single carbohydrate-recognition domain (CRD) with a conserved QPD motif, which was predicted to have galactose specificity. The mRNA expression of PtCLec2 was predominantly detected in intestine and increased rapidly and significantly upon pathogen challenge. Read More

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Identification of the Ricin-B-Lectin LdRBLk in the Colorado Potato Beetle and an Analysis of Its Expression in Response to Fungal Infections.

J Fungi (Basel) 2021 May 6;7(5). Epub 2021 May 6.

Institute of Systematics and Ecology of Animals of Siberian Branch of the Russian Academy of Science, Frunze Str. 11, 630091 Novosibirsk, Russia.

Ricin-B-lectins (RBLs) have been identified in many groups of organisms, including coleopterans insects, particularly the Colorado potato beetle (LdRBLs). We hypothesized that one of these LdRBLs (LdRBLk) may be involved in the immune response to fungal infections. We performed a theoretical analysis of the structure of this protein. Read More

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Duvernoy's Gland Transcriptomics of the Plains Black-Headed Snake, (Squamata, Colubridae): Unearthing the Venom of Small Rear-Fanged Snakes.

Toxins (Basel) 2021 05 6;13(5). Epub 2021 May 6.

Department of Biological Sciences, Clemson University, Clemson, SC 29634, USA.

The venoms of small rear-fanged snakes (RFS) remain largely unexplored, despite increased recognition of their importance in understanding venom evolution more broadly. Sequencing the transcriptome of venom-producing glands has greatly increased the ability of researchers to examine and characterize the toxin repertoire of small taxa with low venom yields. Here, we use RNA-seq to characterize the Duvernoy's gland transcriptome of the Plains Black-headed Snake, , a small, semi-fossorial colubrid that feeds on a variety of potentially dangerous arthropods including centipedes and spiders. Read More

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Trefoil Factor Family (TFF) Peptides and Their Links to Inflammation: A Re-evaluation and New Medical Perspectives.

Authors:
Werner Hoffmann

Int J Mol Sci 2021 May 6;22(9). Epub 2021 May 6.

Institute of Molecular Biology and Medicinal Chemistry, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120 Magdeburg, Germany.

Trefoil factor family peptides (TFF1, TFF2, TFF3), together with mucins, are typical exocrine products of mucous epithelia. Here, they act as a gastric tumor suppressor (TFF1) or they play different roles in mucosal innate immune defense (TFF2, TFF3). Minute amounts are also secreted as endocrine, e. Read More

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The C-type lectin CD93 controls endothelial cell migration via activation of the Rho family of small GTPases.

Matrix Biol 2021 May 29. Epub 2021 May 29.

Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Italy. Electronic address:

Endothelial cell migration is essential to angiogenesis, enabling the outgrowth of new blood vessels both in physiological and pathological contexts. Migration requires the activation of several signaling pathways, the elucidation of which expands the opportunity to develop new drugs to be used in antiangiogenic therapy. In the proliferating endothelium, the interaction between the transmembrane glycoprotein CD93 and the extracellular matrix activates signaling pathways that regulate cell adhesion, migration, and vascular maturation. Read More

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Identification and characterization of a C-type lectin in turbot (Scophthalmus maximus) which functioning as a pattern recognition receptor that binds and agglutinates various bacteria.

Fish Shellfish Immunol 2021 May 29;115:104-111. Epub 2021 May 29.

School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong Province, 266109, China. Electronic address:

C-type lectins (CTLs) are important pathogen pattern recognition receptors that recognize carbohydrate structures. In present study, a C-type lectin domain family 4 member E-like gene from turbot, which tentatively named SmCLEC4E-like (SmCLEC4EL), was identified, and the expressional and functional analyses were performed. In our results, SmCLEC4EL showed conserved synteny with CLEC4E-like genes from several fish species in genome, and possessed a typical type II transmembrane CTL architecture: an N-terminal intracellular region, a transmembrane domain and a C-terminal extracellular region which contained a predicted carbohydrate recognition domain (CRD). Read More

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Poly-L-lysine Glycoconjugates Inhibit DC-SIGN-mediated Attachment of Pandemic Viruses.

ChemMedChem 2021 Jun 1. Epub 2021 Jun 1.

University of Basel: Universitat Basel, Pharmaceutical Sciences, 4056, Basel, SWITZERLAND.

The C-type lectin receptor DC-SIGN mediates interactions with envelope glycoproteins of many viruses such as SARS-CoV-2, ebola, and HIV and contributes to virus internalization and dissemination. In the context of the recent SARS-CoV-2 pandemic, involvement of DC-SIGN has been linked to severe cases of COVID-19. Inhibition of the interaction between DC-SIGN and viral glycoproteins has the potential to generate broad spectrum antiviral agents. Read More

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Role of host factors in SARS-CoV-2 entry.

J Biol Chem 2021 May 28:100847. Epub 2021 May 28.

Center for Retrovirus Research, The Ohio State University, Columbus, Ohio, USA; Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio, USA; Department of Microbial Infection and Immunity, The Ohio State University, Columbus, Ohio, USA; Viruses and Emerging Pathogens Program, Infectious Diseases Institute, The Ohio State University, Columbus, Ohio, USA. Electronic address:

The zoonotic transmission of highly pathogenic coronaviruses into the human population is a pressing concern highlighted by the ongoing SARS-CoV-2 pandemic. Recent work has helped to illuminate much about the mechanisms of SARS-CoV-2 entry into the cell, which determines host- and tissue-specific tropism, pathogenicity, and zoonotic transmission. Here we discuss current findings on the factors governing SARS-CoV-2 entry. Read More

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Human Surfactant Protein D Binds Spike Protein and Acts as an Entry Inhibitor of SARS-CoV-2 Pseudotyped Viral Particles.

Front Immunol 2021 14;12:641360. Epub 2021 May 14.

Center for Allergy & Clinical Immunology Research (ACIR), National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.

Human SP-D is a potent innate immune molecule whose presence at pulmonary mucosal surfaces allows its role in immune surveillance against pathogens. Higher levels of serum SP-D have been reported in the patients with severe acute respiratory syndrome coronavirus (SARS-CoV). Studies have suggested the ability of human SP-D to recognise spike glycoprotein of SARS-CoV; its interaction with HCoV-229E strain leads to viral inhibition in human bronchial epithelial (16HBE) cells. Read More

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Carbohydrates in allergy: from disease to novel immunotherapies.

Trends Immunol 2021 May 27. Epub 2021 May 27.

Department of Experimental Immunology, Amsterdam UMC, location Academic Medical Center (AMC), Amsterdam, The Netherlands; Amsterdam Infection and Immunity Institute, Amsterdam, The Netherlands. Electronic address:

Respiratory allergic disorders are a global public health problem that are responsible for substantial morbidity and healthcare expenditure. Despite the availability of allergen immunotherapy (AIT), its efficacy is suboptimal and regimens are lengthy, with a significant risk of potentially severe side effects. Studies on the recognition of allergens by immune cells through carbohydrate-lectin interactions, which play a crucial role in immune modulation and pathogenesis of allergy, have paved the way for improvements in AIT. Read More

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SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2.

Immunity 2021 06 9;54(6):1304-1319.e9. Epub 2021 May 9.

Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06519, USA.

Despite mounting evidence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) engagement with immune cells, most express little, if any, of the canonical receptor of SARS-CoV-2, angiotensin-converting enzyme 2 (ACE2). Here, using a myeloid cell receptor-focused ectopic expression screen, we identified several C-type lectins (DC-SIGN, L-SIGN, LSECtin, ASGR1, and CLEC10A) and Tweety family member 2 (TTYH2) as glycan-dependent binding partners of the SARS-CoV-2 spike. Except for TTYH2, these molecules primarily interacted with spike via regions outside of the receptor-binding domain. Read More

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CLEC-1 Acts as a Negative Regulator of Dectin-1 Induced Host Inflammatory Response Signature in Aspergillus fumigatus Keratitis.

Invest Ophthalmol Vis Sci 2021 May;62(6):28

Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao, China.

Purpose: C-type lectin-like receptor-1 (CLEC-1) is a member of the Dectin-1 cluster of pattern recognition receptors (PRRs). It is involved in host immunity, has immunoregulatory function, and supports allograft tolerance. Our study aimed to describe the role of CLEC-1 in response to fungal keratitis, in situ, in vivo, and in vitro. Read More

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Identifying potential drug targets and candidate drugs for COVID-19: biological networks and structural modeling approaches.

F1000Res 2021;10:127. Epub 2021 Feb 18.

Centre of Interdisciplinary Science-Computational Life Sciences, College of Chemistry and Chemical Engineering,, Henan University of Technology, Zhengzhou, Henan, 450001, China.

Coronavirus (CoV) is an emerging human pathogen causing severe acute respiratory syndrome (SARS) around the world. Earlier identification of biomarkers for SARS can facilitate detection and reduce the mortality rate of the disease. Thus, by integrated network analysis and structural modeling approach, we aimed to explore the potential drug targets and the candidate drugs for coronavirus medicated SARS. Read More

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Expression of the human or porcine C-type lectins DC-SIGN/L-SIGN confers susceptibility to porcine epidemic diarrhea virus entry and infection in otherwise refractory cell lines.

Microb Pathog 2021 Aug 19;157:104956. Epub 2021 May 19.

Institute of Preventive Veterinary Sciences, Department of Veterinary Medicine, Zhejiang University, Hangzhou 310058, China; Zhaoqing Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Zhaoqing 526238, China. Electronic address:

Porcine epidemic diarrhea virus (PEDV) is an alphacoronavirus that causes great economic losses in the porcine industry. Although the functional receptor for the virus has not been identified, multiple isolates are able to infect different cell lines. Recently, it has been shown that the human C-type lectin DC-SIGN/L-SIGN (hDC-SIGN/L-SIGN) can promote entry of several coronaviruses. Read More

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A humoral factor, hemolymph proteinase 8, elicits a cellular defense response of nodule formation in Bombyx mori larvae in association with recognition by C-type lectins.

J Insect Physiol 2021 May 20;132:104252. Epub 2021 May 20.

Graduate School of Bio-Application and Systems Engineering, Tokyo University of Agriculture and Technology, Naka-cho 2-24-16, Koganei, Tokyo 184-8588, Japan. Electronic address:

Previously, we found that nodule formation, a cellular defense response in insects, is regulated by humoral factors called C-type lectins in the hemolymph. To elucidate the factors that elicit nodule formation following the recognition of microorganisms by C-type lectins, a reproducible quantitative in vitro assay system was constructed. Then, using this system, the inhibitory activities of antisera raised against hemolymph proteases (HPs), serine protease homologues (SPHs), and pathogen-associated molecular pattern (PAMP)-recognition proteins were assessed. Read More

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Selective expression of a C-type lectin receptor, Clec12b, on skin mast cells.

Biochem Biophys Res Commun 2021 Jul 18;561:101-105. Epub 2021 May 18.

Department of Immunology, Faculty of Medicine, University of Tsukuba, Japan; Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Japan; R&D Center for Innovative Drug Discovery, University of Tsukuba, Japan. Electronic address:

Mast cells (MCs) are present in various organs including the skin, peritoneal cavity, lung, and intestine and involved in the development of allergic diseases and host defense against infection. However, the regulatory mechanism of mast cell activation remains incompletely understood. We found in a database that Clec12b encoding a C-type lectin receptor Clec12b is preferentially expressed in skin MCs in mice. Read More

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