380 results match your criteria bullfrog skin

Sustainable aquaculture side-streams derived hybrid biocomposite for bone tissue engineering.

Mater Sci Eng C Mater Biol Appl 2021 Jul 17;126:112104. Epub 2021 Apr 17.

School of Materials Science and Engineering, Nanyang Technological University Singapore, N4.1, 50 Nanyang Avenue, Singapore 639798, Singapore; Environmental Chemistry and Materials Centre, Nanyang Environment & Water Research Institute, 1 CleanTech Loop, CleanTech One, Singapore 637141, Singapore; School of Biological Sciences, Nanyang Technological University Singapore, 60 Nanyang Drive, Singapore 637551, Singapore; Energy Research Institute, Nanyang Technological University Singapore, 50 Nanyang Drive, Singapore 637553, Singapore. Electronic address:

Despite being a rich source of bioactive compounds, the current exploitation of aquatic biomass is insufficient. Majority of the aquaculture industry side-streams are currently used for low-value purposes such as animal feed or composting material, with low economical returns. To maximize resource reuse and minimize waste generation, valorization efforts should be augmented with the aim to produce high-value products. Read More

View Article and Full-Text PDF

Antineoplastic Effects and Mechanisms of a New RGD Chimeric Peptide from Bullfrog Skin on the Proliferation and Apoptosis of B16F10 Cells.

Protein J 2021 Apr 20. Epub 2021 Apr 20.

Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.

Malignant melanoma, an increasingly common form of skin cancer, poses a significant threat to public health, especially when the disease progresses past skin lesions to the stage of advanced metastasis. In this work, a new anti-tumor peptide, temporin La (T-La), was selected from a cDNA library generated from bullfrog skin. Two new derivative antitumor peptides, T-La (S) and T-La (FS), were designed by bioinformatics analysis and coupled with the RGD small molecule peptide to create chimeric RGD peptides, (RGD-T-La [S] and RGD-T-La [FS]). Read More

View Article and Full-Text PDF

Sucralose Affects Thyroid Hormone Signaling in American Bullfrog [Rana (Lithobates) catesbeiana] Tadpoles.

Arch Environ Contam Toxicol 2021 May 31;80(4):735-744. Epub 2021 Mar 31.

Department of Biochemistry and Microbiology, University of Victoria, 3800 Finnerty Road, Victoria, BC, V8P 5C2, Canada.

Nonnutritive sweeteners used in food and beverage products are widespread, persistent aquatic pollutants. Despite this, their impact on aquatic organisms, particularly vertebrates, is not well-studied. Recent findings in rodents suggest sucralose, a chlorinated disaccharide, alters thyroid hormone (TH) metabolism. Read More

View Article and Full-Text PDF

Genotoxic and melanic alterations in Lithobates catesbeianus (anura) tadpoles exposed to fipronil insecticide.

Environ Sci Pollut Res Int 2021 Apr 6;28(16):20072-20081. Epub 2021 Jan 6.

Laboratório de Patologia Experimental, Universidade Federal de Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.

The aim of this study was to evaluate the genotoxic and morphological systemic effects of both an acute and a chronic exposure of bullfrog tadpoles to fipronil. Lithobates catesbeianus tadpoles had morphological biomarkers (skin, liver, and blood) analyzed at Gosner stages 36-38, when exposed to four different concentrations of Regent® 800 WG (80% fipronil): 0.00 (control), 0. Read More

View Article and Full-Text PDF

Molecular design and anti-melanoma activity of a novel bullfrog antibacterial peptide RGD-chimera.

Oncol Lett 2021 Feb 15;21(2):115. Epub 2020 Dec 15.

Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, P.R. China.

Melanoma is a common malignant skin tumor, which is the only fatal skin tumor at present. Melanoma has a high degree of malignancy and metastasis. The activity of modified Temporin-La (T-La) peptides from bullfrog skin were evaluated for antitumor activity and improved targeting in melanoma cells. Read More

View Article and Full-Text PDF
February 2021

Hepatotoxicity of the anionic surfactant linear alkylbenzene sulphonate (LAS) in bullfrog tadpoles.

Chemosphere 2021 Mar 18;266:129014. Epub 2020 Nov 18.

Departamento de Biologia, Universidade Estadual Paulista (UNESP), São José do Rio Preto, São Paulo, Brazil.

The liver of anurans play an important role in metabolism, including detoxification, the biotransformation of molecules, and the storage of metabolites. Surfactants are part of domestic and industrial effluents. The effects of linear alkylbenzene sulfonate (LAS) on anuran liver remain unknown, however, some studies have evaluated the effects of LAS on the skin, gills, heart, testes, and liver of fishes. Read More

View Article and Full-Text PDF

Evaluation of the potential teratogenic and toxic effect of the herbicide 2,4-D (DMA® 806) in bullfrog embryos and tadpoles (Lithobates catesbeianus).

Chemosphere 2021 Mar 19;266:129018. Epub 2020 Nov 19.

Fisheries Institute - APTA - SAA, 455 Francisco Matarazzo Ave., 05001-900, São Paulo, SP, Brazil.

The objective of this work was to evaluate the potential teratogenic and toxic effect of the herbicide 2,4-D (DMA® 806) on bullfrog embryos and tadpoles (Lithobates catesbeianus). We used the FETAX (Frog Embryo Teratogenesis Assay Xenopus) assay for embryos, and for tadpoles, we used acute (96 h) and chronic (49 days) toxicity tests and evaluated aspects of healthiness, hematology, and histopathology. The LC (Median Lethal Concentration), EC (Median Effective Concentration), MCIG (Minimum Concentration to Inhibit Growth) and TI (Teratogenic Index) for embryos were 792 mg/L, 593 mg/L, 150 mg/L of 2,4-D (DMA) and 1. Read More

View Article and Full-Text PDF

Asian bullfrog (Rana tigerina) skin gelatin extracted by ultrasound-assisted process: Characteristics and in-vitro cytotoxicity.

Int J Biol Macromol 2020 Apr 16;148:391-400. Epub 2020 Jan 16.

Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.

Impact of ultrasound-assisted process (UAP) on yield and characteristics of Asian bullfrog (Rana tigerina) skin gelatin was studied and the in-vitro cytotoxicity of the resulting frog skin gelatin was evaluated using CaCo-2, Raw264.7 and L929 cell lines. Different ultrasonication modes (5 min on-time with 25 min off-time (5/25) and 10 min on-time with 50 min off-times (10/50)) were implemented for different total ultrasonication times (10-60 min) during gelatin extraction at 55 °C. Read More

View Article and Full-Text PDF

Sera/Organ Lysates of Selected Animals Living in Polluted Environments Exhibit Cytotoxicity against Cancer Cell Lines.

Anticancer Agents Med Chem 2019 ;19(18):2251-2268

Department of Biology, Chemistry and Environmental Sciences, College of Arts and Sciences, American University of Sharjah, University City, Sharjah, United Arab Emirates.

Background: Species of crocodiles and cockroaches can withstand high radiation, reside in unsanitary conditions, thrive on germ-infested feed, and are exposed to heavy metals, yet they are not reported to develop cancer. It has been postulated that such species have mechanisms to defend themselves against developing cancer. Here, selected species have been tested for potential cytotoxicity against selected cancer cell lines. Read More

View Article and Full-Text PDF
September 2020

Skin-associated lactic acid bacteria from North American bullfrogs as potential control agents of Batrachochytrium dendrobatidis.

PLoS One 2019 27;14(9):e0223020. Epub 2019 Sep 27.

Instituto Superior de Investigaciones Biológicas (INSIBIO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Instituto de Biología "Dr. Francisco D. Barbieri", Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán (UNT), San Miguel de Tucumán, Argentina.

The fungal pathogen Batrachochytrium dendrobatidis (Bd) is the causative agent of chytridiomycosis and has been a key driver in the catastrophic decline of amphibians globally. While many strategies have been proposed to mitigate Bd outbreaks, few have been successful. In recent years, the use of probiotic formulations that protect an amphibian host by killing or inhibiting Bd have shown promise as an effective chytridiomycosis control strategy. Read More

View Article and Full-Text PDF

Replacement of l-Amino Acids by d-Amino Acids in the Antimicrobial Peptide Ranalexin and Its Consequences for Antimicrobial Activity and Biodistribution.

Molecules 2019 Aug 17;24(16). Epub 2019 Aug 17.

Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, 69120 Heidelberg, Germany.

Infections caused by multidrug-resistant bacteria are a global emerging problem. New antibiotics that rely on innovative modes of action are urgently needed. Ranalexin is a potent antimicrobial peptide (AMP) produced in the skin of the American bullfrog . Read More

View Article and Full-Text PDF

Effects of 2,4-D-based herbicide (DMA 806) on sensitivity, respiration rates, energy reserves and behavior of tadpoles.

Ecotoxicol Environ Saf 2019 Oct 16;182:109446. Epub 2019 Jul 16.

NEEA/CRHEA/SHS, São Carlos Engineering School, University of São Paulo, São Carlos, SP, Brazil.

Increased use of sugarcane pesticides and their destination to non-target environments in Brazil has generated concerns related to the conservation of more vulnerable groups, such as amphibians. Besides the high skin permeability, tadpoles are constantly restricted to small and ephemeral ponds, where exposure to high concentrations of pesticides in agricultural areas is inevitable. This study evaluated chronic effects caused by sub-lethal concentrations of 2,4-dichlorophenoxyacetic acid herbicide on energy storage, development, respiration rates, swimming performance and avoidance behavior of bullfrog tadpoles (Lithobates catesbeianus). Read More

View Article and Full-Text PDF
October 2019

Discovery of Wild Amphibians Infected with Ranavirus in Brazil.

J Wildl Dis 2019 10 13;55(4):897-902. Epub 2019 May 13.

Laboratório de História Natural de Anfíbios Brasileiros, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil, CEP 13083-862.

Ranavirus is a double-stranded DNA virus associated with amphibian, fish and reptile die-offs worldwide. International trade of live animals farmed for human consumption, such as the American bullfrog (), plays a key role in spreading the pathogen. In Brazil, ranavirus has only been reported in captive bullfrog farms. Read More

View Article and Full-Text PDF
October 2019

Tadpole serum activity ( a) in caspase-3 as a marker of the role of apoptosis and total cytotoxic T lymphocytes in albino rats' epithelial cells induced by neoplasia.

Vet World 2019 Jan 10;12(1):63-67. Epub 2019 Jan 10.

Department of Veterinary Pathology, Faculty of Veterinary Medicine, Universitas Airlangga, Mulyorejo Campus C Surabaya, East Java 60115, Indonesia.

Aim: This study was conducted to examine the tadpole's serum activity () in caspase-3 as a marker of the role of apoptosis and total cytotoxic T lymphocyte (CTL) in albino rats' epithelial cells induced by neoplasia. Tadpole serumcontains thyroxine hormone that may cause the metamorphosis process and control cell proliferation.

Materials And Methods: Male rats were induced by 7,12-dimethylbenz (α)anthracene (DMBA) 20 mg/rats twice every week over 5 weeks to stimulate skin neoplasia. Read More

View Article and Full-Text PDF
January 2019

Antimicrobial peptides from Rana [Lithobates] catesbeiana: Gene structure and bioinformatic identification of novel forms from tadpoles.

Sci Rep 2019 02 6;9(1):1529. Epub 2019 Feb 6.

Canada's Michael Smith Genome Sciences Centre, BC Cancer Agency, Vancouver, BC, V5Z 4S6, Canada.

Antimicrobial peptides (AMPs) exhibit broad-spectrum antimicrobial activity, and have promise as new therapeutic agents. While the adult North American bullfrog (Rana [Lithobates] catesbeiana) is a prolific source of high-potency AMPs, the aquatic tadpole represents a relatively untapped source for new AMP discovery. The recent publication of the bullfrog genome and transcriptomic resources provides an opportune bridge between known AMPs and bioinformatics-based AMP discovery. Read More

View Article and Full-Text PDF
February 2019

Pathogen Risk Analysis for Wild Amphibian Populations Following the First Report of a Ranavirus Outbreak in Farmed American Bullfrogs () from Northern Mexico.

Viruses 2019 01 3;11(1). Epub 2019 Jan 3.

Department of Aquaculture, Instituto Politécnico, Nacional-CIIDIR Unidad Sinaloa, Guasave Sinaloa 81101, Mexico.

Ranaviruses are the second deadliest pathogens for amphibian populations throughout the world. Despite their wide distribution in America, these viruses have never been reported in Mexico, the country with the fifth highest amphibian diversity in the world. This paper is the first to address an outbreak of ranavirus in captive American bullfrogs () from Sinaloa, Mexico. Read More

View Article and Full-Text PDF
January 2019

Opening the file drawer: Unexpected insights from a chytrid infection experiment.

PLoS One 2018 9;13(5):e0196851. Epub 2018 May 9.

Department of Environmental Science, Policy & Management, University of California, Berkeley, California, United States of America.

Infection experiments are critical for understanding wildlife disease dynamics. Although infection experiments are typically designed to reduce complexity, disease outcomes still result from complex interactions between host, pathogen, and environmental factors. Cryptic variation across factors can lead to decreased repeatability of infection experiments within and between research groups and hinder research progress. Read More

View Article and Full-Text PDF

Study of biological activity and targeted distribution of catesbeianalectin in mouse tissue.

Pak J Pharm Sci 2018 May;31(3(Supplementary)):1013-1020

College of Animal Science and Veterinary Medicine, Tianjin Agriculture University, Tianjin, China.

Lectin has attracted attention because of its ability to serve as a carrier for targeted drug delivery. Large lectins isolated from marine invertebrates and crustaceans have strong immunogenicity and adverse effects, which limit their usefulness. This study reports the identification of catesbeianalectin via screening a bullfrog skin cDNA library. Read More

View Article and Full-Text PDF

Introduced bullfrog facilitates pathogen invasion in the western United States.

PLoS One 2018 16;13(4):e0188384. Epub 2018 Apr 16.

Museum of Vertebrate Zoology, University of California, Berkeley, California, United States of America.

Batrachochytrium dendrobatidis (Bd), a causal agent of the amphibian fungal skin disease chytridiomycosis, has been implicated in the decline and extinction of over 200 species worldwide since the 1970s. Despite almost two decades of research, the history of Bd and its global spread is not well understood. However, the spread of the Global Panzootic Lineage of Bd (Bd-GPL), the lineage associated with amphibian die-offs, has been linked with the American bullfrog (Rana [Aqurana] catesbeiana) and global trade. Read More

View Article and Full-Text PDF

A novel tool against multiresistant bacterial pathogens: lipopeptide modification of the natural antimicrobial peptide ranalexin for enhanced antimicrobial activity and improved pharmacokinetics.

Int J Antimicrob Agents 2018 Jul 9;52(1):52-62. Epub 2018 Apr 9.

Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, 69120 Heidelberg, Germany. Electronic address:

As evolutionarily optimised defence compounds, antimicrobial peptides (AMPs) represent a powerful tool against bacterial infections. Ranalexin, an AMP found in the skin of the American bullfrog (Rana catesbeiana), is primarily active against Gram-positive bacteria, with minimum inhibitory concentrations (MICs) of 8-16 mg/L, but shows weaker activity against Gram-negative bacteria (MICs > 64 mg/L). By substitution of six N-terminal amino acids by saturated fatty acids [decanoic acid (C10:0) to myristic acid (C14:0)], lipopeptide derivatives with enhanced antimicrobial activity were developed. Read More

View Article and Full-Text PDF

Gene expression differs in susceptible and resistant amphibians exposed to .

R Soc Open Sci 2018 Feb 28;5(2):170910. Epub 2018 Feb 28.

Department of Wildlife, Fish, and Conservation Biology, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.

Chytridiomycosis, the disease caused by the fungal pathogen (), has devastated global amphibian biodiversity. Nevertheless, some hosts avoid disease after exposure even as others experience near-complete extirpation. It remains unclear whether the amphibian adaptive immune system plays a role in defence. Read More

View Article and Full-Text PDF
February 2018

UV-B affects the immune system and promotes nuclear abnormalities in pigmented and non-pigmented bullfrog tadpoles.

J Photochem Photobiol B 2018 Mar 31;180:109-117. Epub 2018 Jan 31.

Department of Biology, São Paulo State University (UNESP), São José do Rio Preto, São Paulo 15054-000, Brazil.

Ultra-Violet (UV) radiation is a stressor of the immune system and causes DNA damage. Leukocytes can change in response to environmental changes in anurans, making them an important biomarker of stressful situations. The initial barrier against UV in ectothermic animals is melanin-containing cells in skin and in their internal organs. Read More

View Article and Full-Text PDF

Effect of ultra-high pressure on molecular structure and properties of bullfrog skin collagen.

Int J Biol Macromol 2018 May 4;111:200-207. Epub 2018 Jan 4.

School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Changqing Garden, Wuhan, Hubei, China.

Ultra-high pressure technology has attracted a great deal of attention in recent years, and has been widely used in food science, medicine, and other fields. This study aimed to determine the effect of ultra-high pressure on the structure and properties of collagen. Native collagen extracted from bullfrog skin was processed under different ultra-high pressure treatment conditions (300, 400, and 500MPa). Read More

View Article and Full-Text PDF

Ranatensin-HL: A Bombesin-Related Tridecapeptide from the Skin Secretion of the Broad-Folded Frog, Hylarana latouchii.

Molecules 2017 Jul 4;22(7). Epub 2017 Jul 4.

Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK.

Bombesin-related peptides are a family of peptides whose prototype was discovered in amphibian skin and which exhibit a wide range of biological activities. Since the initial isolation of bombesin from skin, diverse forms of bombesin-related peptides have been found in the skins across Anura. In this study, a novel bombesin-related peptide of the ranatensin subfamily, named ranatensin-HL, was structurally-characterised from the skin secretion of the broad-folded frog, , through combination of molecular cloning and mass spectrometric methodologies. Read More

View Article and Full-Text PDF

Dominance-function relationships in the amphibian skin microbiome.

Environ Microbiol 2017 08 26;19(8):3387-3397. Epub 2017 Jul 26.

Department of Biological Sciences, Virginia Tech, Blacksburg, VA, USA.

Some amphibian skin bacteria inhibit growth of a fungal amphibian pathogen, Batrachochytrium dendrobatidis (Bd), but it is unclear how dominant these anti-Bd bacteria are in skin communities. Using in vitro co-culture challenge assays, we quantified Bd inhibition by bacterial isolates collected from the skin of four amphibian species: bullfrogs, Eastern newts, spring peepers and American toads. The 16S rRNA sequences for each isolate were matched to culture-independent amplicon sequences from the same individuals to assess inhibitory function versus relative abundance. Read More

View Article and Full-Text PDF

Current knowledge of ghrelin in amphibians.

Endocr J 2017 ;64(Suppl.):S15-S19

Department of Biochemistry, National Cerebral and Cardiovascular Center Research Institute, Suita 565-8565, Japan.

We are exploring physiological importance of the ghrelin system in vertebrates. This review summarizes current knowledge of the ghrelin system in amphibians. Our study on ghrelin precursor in various amphibians revealed that the third amino acid with acyl modification has changed to threonine (Thr-3) instead of serine (Ser-3) only in the genus, Rana. Read More

View Article and Full-Text PDF

The genotoxicity and cytotoxicity of tannery effluent in bullfrog (Lithobates catesbeianus).

Chemosphere 2017 Sep 16;183:491-502. Epub 2017 May 16.

Departamento de Ciências Biológicas, Laboratório de Pesquisas Biológicas and Programa de Pós-Graduação em Conservação de Recursos Naturais do Cerrado, Instituto Federal Goiano - Campus Urutaí, GO, Brazil; Programa de Pós-Graduação em Biodiversidade Animal, Universidade Federal de Goiás - Campus Samambaia, GO, Brazil. Electronic address:

Some of the most polluting activities occur in bovine skin processing. Tannery generates effluents containing high concentrations of heavy metals and organic compounds. The phases composing the leather production process generate a large volume of tannery effluents that are often discarded in aquatic environments without any previous treatment. Read More

View Article and Full-Text PDF
September 2017

Enantioselective degradation of alpha-cypermethrin and detection of its metabolites in bullfrog (rana catesbeiana).

Ecotoxicol Environ Saf 2017 Jul 19;141:93-97. Epub 2017 Mar 19.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Department of Applied Chemistry, China Agricultural University, Beijing 100193, PR China. Electronic address:

Bullfrog, as a kind of amphibians, can be easily exposed to varied pollutants in the environment for the reason of its habitats and highly permeable skin. We investigated the degradation kinetics and residues of α-cypermethrin in bullfrog by two different methods of administration for the environmental monitoring the behavior of one of the most used pesticides in the amphibians. The oral administration and water exposure of α-cypermethrin on bullfrog was studied in this work. Read More

View Article and Full-Text PDF

Catesbeianin-1, a novel antimicrobial peptide isolated from the skin of Lithobates catesbeianus (American bullfrog).

Biotechnol Lett 2017 Jun 3;39(6):897-903. Epub 2017 Mar 3.

College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, Changchun, 130062, People's Republic of China.

Objectives: To identify and characterize a novel antimicrobial peptide, catesbeianin-1.

Results: Catesbeianin-1 is 25 amino acids long and is α-helical, cationic and amphipathic. It had antimicrobial activity against Gram-positive and Gram-negative bacteria. Read More

View Article and Full-Text PDF

Purification, Conformational Analysis, and Properties of a Family of Tigerinin Peptides from Skin Secretions of the Crowned Bullfrog Hoplobatrachus occipitalis.

J Nat Prod 2016 09 25;79(9):2350-6. Epub 2016 Aug 25.

SAAD Centre for Pharmacy and Diabetes, School of Biomedical Sciences, University of Ulster , Coleraine, U.K.

Four host-defense peptides belonging to the tigerinin family (tigerinin-1O: RICTPIPFPMCY; tigerinin-2O: RTCIPIPLVMC; tigerinin-3O: RICTAIPLPMCL; and tigerinin-4O: RTCIPIPPVCF) were isolated from skin secretions of the African crowned bullfrog Hoplobatrachus occipitalis. In aqueous solution at pH 4.8, the cyclic domain of tigerinin-2O adopts a rigid amphipathic conformation that incorporates a flexible N-terminal tail. Read More

View Article and Full-Text PDF
September 2016