Sci Rep 2021 Mar 18;11(1):6298. Epub 2021 Mar 18.
Laboratorio de Biotecnología, Estación Experimental Agropecuaria (EEA) San Pedro, INTA, 2930, San Pedro, Argentina.
The advance of Next Generation Sequencing (NGS) technologies allows high-throughput genotyping at a reasonable cost, although, in the case of peach, this technology has been scarcely developed. To date, only a standard Genotyping by Sequencing approach (GBS), based on a single restriction with ApeKI to reduce genome complexity, has been applied in peach. In this work, we assessed the performance of the double-digest RADseq approach (ddRADseq), by testing 6 double restrictions with the restriction profile generated with ApeKI. Read More