8,483 results match your criteria bpti

Real-time adsorption of optical brightening agents on cellulose thin films.

Carbohydr Polym 2021 Jun 16;261:117826. Epub 2021 Feb 16.

Graz University of Technology, Institute of Bioproducts and Paper Technology (BPTI), Inffeldgasse 23, 8010 Graz, Austria; CD-Laboratory for Fibre Swelling and Paper Performance, Inffeldgasse 23, 8010 Graz, Austria.

Optical brightening agents (OBAs) are commonly used in textile and paper industry to adjust product brightness and color appearence. Continuous production processes lead to short residence time of the dyes in the fiber suspension, making it necessary to understand the kinetics of adsorption. The interaction mechanisms of OBAs with cellulose are challenging to establish as the fibrous nature of cellulosic substrates complicates acquisition of real-time data. Read More

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Renal effects of the serine protease inhibitor aprotinin in healthy conscious mice.

Acta Pharmacol Sin 2021 Mar 23. Epub 2021 Mar 23.

Division of Endocrinology, Diabetology and Nephrology, Department of Internal Medicine, University Hospital Tübingen, Tübingen, Germany.

Treatment with aprotinin, a broad-spectrum serine protease inhibitor with a molecular weight of 6512 Da, was associated with acute kidney injury, which was one of the reasons for withdrawal from the market in 2007. Inhibition of renal serine proteases regulating the epithelial sodium channel ENaC could be a possible mechanism. Herein, we studied the effect of aprotinin in wild-type 129S1/SvImJ mice on sodium handling, tubular function, and integrity under a control and low-salt diet. Read More

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Cardiothoracic Anesthesia and Critical Care in the United Kingdom (UK) Part 1: Some Insights Into the History and Development.

J Cardiothorac Vasc Anesth 2021 Feb 13. Epub 2021 Feb 13.

Department of Cardiothoracic Anaesthesia, Critical Care and Pain Management, Royal Infirmary of Edinburgh, Edinburgh, Scotland.

This review is intended to highlight some of the historic events that contributed to the development of thoracic and cardiac anesthesia and surgery in Great Britain and Northern Ireland (UK). The aim of this first of two parts is to concentrate on the development of techniques, facilities, and pharmacology that allowed progress and advancement in patient management that were developed primarily in the UK. However, progress usually requires input from a wide variety of sources of knowledge, and cardiothoracic practice is no exception. Read More

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February 2021

Synergistic Effect of β-alanine and Aprotinin on Mesenteric Ischemia.

J Surg Res 2021 Feb 24;263:78-88. Epub 2021 Feb 24.

Institut für Physiologische Chemie, Universitätsklinikum Essen, Universität Duisburg-Essen, Essen, Germany. Electronic address:

Background: Acute mesenteric ischemia arises through sudden interruption of mesenteric blood flow, mostly due to an occlusion of the superior mesenteric artery and is associated with a high mortality of approximately 50% to 90%. In previous studies, the single application of β-alanine or aprotinin caused an ameliorated intestinal damage but without any systemic effects.

Methods: To analyze the combined effect of β-alanine and aprotinin on acute ischemia and reperfusion of the small intestine, a model with anesthetized rats was used. Read More

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February 2021

Sialotranscriptomics of the argasid tick Ornithodoros moubata along the trophogonic cycle.

PLoS Negl Trop Dis 2021 Feb 5;15(2):e0009105. Epub 2021 Feb 5.

Parasitology Laboratory, Institute of Natural Resources and Agrobiology (IRNASA, CSIC), Cordel de Merinas, Salamanca, Spain.

The argasid tick Ornithodoros moubata is the main vector of human relapsing fever (HRF) and African swine fever (ASF) in Africa. Salivary proteins are part of the host-tick interface and play vital roles in the tick feeding process and the host infection by tick-borne pathogens; they represent interesting targets for immune interventions aimed at tick control. The present work describes the transcriptome profile of salivary glands of O. Read More

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February 2021

Circulating Chromogranin A Is Cleaved Into Vasoregulatory Fragments in Patients With Pancreatic Ductal Adenocarcinoma.

Front Oncol 2020 23;10:613582. Epub 2020 Dec 23.

Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Milan, Italy.

Chromogranin A (CgA), a secretory protein released in the blood by the neuroendocrine system, consists of a mixture of full-length molecules and fragments endowed of vasoregulatory activity. The extent and the role of CgA fragmentation were investigated in patients with locally advanced or metastatic pancreatic ductal adenocarcinoma (PDAC, n=172). Multivariate analysis showed that full-length CgA was associated with better progression free and overall survival, whereas CgA C-terminal fragmentation was associated with worse prognosis. Read More

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December 2020

Structural modelling and thermostability of a serine protease inhibitor belonging to the Kunitz-BPTI family from the Rhipicephalus microplus tick.

Biochimie 2021 Feb 24;181:226-233. Epub 2020 Dec 24.

Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, São Bernardo do Campo, São Paulo, Brazil. Electronic address:

rBmTI-A is a recombinant serine protease inhibitor that belongs to the Kunitz-BPTI family and that was cloned from Rhipicephalus microplus tick. rBmTI-A has inhibitory activities on bovine trypsin, human plasma kallikrein, human neutrophil elastase and plasmin with dissociation constants in nM range. It is characterized by two inhibitory domains and each domain presents six cysteines that form three disulfide bonds, which contribute to the high stability of its structure. Read More

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February 2021

Protein repellent anti-coagulative mixed-charged cellulose derivative coatings.

Carbohydr Polym 2021 Feb 25;254:117437. Epub 2020 Nov 25.

Institute for Chemistry and Technology of Biobased Systems, Graz University of Technology, Stremayrgasse 9, A-8010, Graz, Austria.

This study describes the formation of cellulose based polyelectrolyte charge complexes on the surface of biodegradable polycaprolactone (PCL) thin films. Anionic sulphated cellulose (CS) and protonated cationic amino cellulose (AC) were used to form these complexes with a layer-by-layer coating technique. Both polyelectrolytes were analyzed by charge titration methods to elucidate their pH-value dependent protonation behavior. Read More

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February 2021

Establishing reference ranges of cord blood: point-of-care hemostatic function assessment in preterm and term neonates.

Pediatr Res 2020 Dec 18. Epub 2020 Dec 18.

Department of Anesthesia, Critical Care and Pain Medicine, Division of General Anesthesia and Intensive Care Medicine, Medical University of Vienna, 1090, Vienna, Austria.

Background: Thrombelastometry, allowing timely assessment of global hemostatic function, is increasingly used to guide hemostatic interventions in bleeding patients. Reference values are available for adults and children, including infants but not neonates immediately after birth.

Methods: Neonates were grouped as preterm (30 + 0 to 36 + 6 weeks/days) and term (37 + 0 to 39 + 6 weeks/days). Read More

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December 2020

A randomized, open-label, adaptive, proof-of-concept clinical trial of modulation of host thromboinflammatory response in patients with COVID-19: the DAWn-Antico study.

Trials 2020 Dec 9;21(1):1005. Epub 2020 Dec 9.

Center for Molecular and Vascular Biology, KU Leuven Department of Cardiovascular Sciences, KU Leuven, Leuven, Belgium.

Background: The peak of the global COVID-19 pandemic has not yet been reached, and many countries face the prospect of a second wave of infections before effective vaccinations will be available. After an initial phase of viral replication, some patients develop a second illness phase in which the host thrombotic and inflammatory responses seem to drive complications. Severe COVID-19 disease is linked to high mortality, hyperinflammation, and a remarkably high incidence of thrombotic events. Read More

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December 2020

Overhydration Measured by Bioimpedance Spectroscopy and Urinary Serine Protease Activity Are Risk Factors for Progression of Chronic Kidney Disease.

Kidney Blood Press Res 2020 2;45(6):955-968. Epub 2020 Dec 2.

Department of Internal Medicine IV, Division of Diabetology, Endocrinology and Nephrology, University Hospital Tübingen, Tübingen, Germany.

Background: Overhydration (OH) is common in chronic kidney disease (CKD) and might be related to the excretion of urinary serine proteases. Progression of CKD is associated with proteinuria; however, the interrelations of urinary serine proteases, OH, and progression of CKD remain unclear.

Methods: In n = 179 patients with stable nondialysis-dependent CKD of all stages, OH was measured using bioimpedance spectroscopy (Body Composition Monitor; Fresenius), and urinary serine protease activity was determined using the peptide substrate S-2302. Read More

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December 2020

A hybrid scaffold of gelatin glycosaminoglycan matrix and fibrin as a carrier of human corneal fibroblast cells.

Mater Sci Eng C Mater Biol Appl 2021 Jan 22;118:111430. Epub 2020 Aug 22.

Department of Chemical Engineering, Ryerson University, Toronto, Canada.

A hybrid scaffold of gelatin-glycosaminoglycan matrix and fibrin (FGG) has been synthesized to improve the mechanical properties, degradation time and cell response of fibrin-like scaffolds. The FGG scaffold was fabricated by optimizing some properties of fibrin-only gel and gelatin-glycosaminoglycan (GG) scaffolds. Mechanical analysis of optimized fibrin-only gel showed the Young module and tensile strength of up to 72 and 121 KPa, respectively. Read More

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January 2021

Methylene blue may have a role in the treatment of COVID-19.

Med Hypotheses 2020 Nov 6;144:110163. Epub 2020 Aug 6.

Shahid Beheshti University of Medical Sciences(SBMU), Tehran, Iran.

In this paper, we raise the hypothesis that Methylene Blue may be a treatment option for Corona Virus Disease of 2019 specially when combined with Non Steroid Anti-Inflammatory Drugs. In previous publications including ours, the role of kininogen system has been postulated. A correlation between clinical findings of the disease and this mechanism has been drawn to denote a pivotal role of kininogen-kallikrein system in pathophysiology of the disease. Read More

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November 2020

Enhanced Antifibrinolytic Efficacy of a Plasmin-Specific Kunitz-Inhibitor (60-Residue Y11T/L17R with C-Terminal IEK) of Human Tissue Factor Pathway Inhibitor Type-2 Domain1.

J Clin Med 2020 Nov 17;9(11). Epub 2020 Nov 17.

Department of Orthopedic Surgery, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.

Current antifibrinolytic agents reduce blood loss by inhibiting plasmin active sites (e.g., aprotinin) or by preventing plasminogen/tissue plasminogen activator (tPA) binding to fibrin clots (e. Read More

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November 2020

Revisiting the Formation of a Native Disulfide Bond: Consequences for Protein Regeneration and Beyond.

Mahesh Narayan

Molecules 2020 Nov 16;25(22). Epub 2020 Nov 16.

The Department of Chemistry and Biochemistry, The University of Texas as El Paso, El Paso, TX 79968, USA.

Oxidative protein folding involves the formation of disulfide bonds and the regeneration of native structure (N) from the fully reduced and unfolded protein (R). Oxidative protein folding studies have provided a wealth of information on underlying physico-chemical reactions by which disulfide-bond-containing proteins acquire their catalytically active form. Initially, we review key events underlying oxidative protein folding using bovine pancreatic ribonuclease A (RNase A), bovine pancreatic trypsin inhibitor (BPTI) and hen-egg white lysozyme (HEWL) as model disulfide bond-containing folders and discuss consequential outcomes with regard to their folding trajectories. Read More

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November 2020

Kunitz-Type Peptides from the Sea Anemone Demonstrate Potassium Channel Blocking and Anti-Inflammatory Activities.

Biomedicines 2020 Nov 4;8(11). Epub 2020 Nov 4.

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159, Pr. 100 let Vladivostoku, 690022 Vladivostok, Russian.

The Kunitz/BPTI peptide family includes unique representatives demonstrating various biological activities. Electrophysiological screening of peptides HCRG1 and HCRG2 from the sea anemone on six Kv1.x channel isoforms and insect IR channel expressed in oocytes revealed their potassium channels blocking activity. Read More

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November 2020

Injured tissues favor cancer cell implantation via fibrin deposits on scar zones.

Neoplasia 2020 12 3;22(12):809-819. Epub 2020 Nov 3.

CAP-Paris Tech, INSERM U1275, Université de Paris, Lariboisière Hospital, 2 rue Ambroise Paré, 75010 Paris, France. Electronic address:

Aim: Evaluation of fibrin role on cancer cells implantation in injured tissues and studying the molecular mechanism of cancer cell interaction with the peritoneal damage.

Material And Methods: Mouse colon cancer (CT26) and human mesothelial cells (HMCs) were used. CT26 cells were implanted on injured peritoneal zones. Read More

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December 2020

Aprotinin Inhibits SARS-CoV-2 Replication.

Cells 2020 10 30;9(11). Epub 2020 Oct 30.

Institute for Medical Virology, University Hospital, Goethe University, 60596 Frankfurt am Main, Germany.

Severe acute respiratory syndrome virus 2 (SARS-CoV-2) is the cause of the current coronavirus disease 19 (COVID-19) pandemic. Protease inhibitors are under consideration as virus entry inhibitors that prevent the cleavage of the coronavirus spike (S) protein by cellular proteases. Herein, we showed that the protease inhibitor aprotinin (but not the protease inhibitor SERPINA1/alpha-1 antitrypsin) inhibited SARS-CoV-2 replication in therapeutically achievable concentrations. Read More

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October 2020

A new decellularized tendon scaffold for rotator cuff tears - evaluation in rabbits.

BMC Musculoskelet Disord 2020 Oct 17;21(1):689. Epub 2020 Oct 17.

Department of Orthopedic and Traumatology, EPM - Federal University from São Paulo, 715 Napoleão de Barros, São Paulo, SP, 04038-000, Brazil.

Background: Scaffolds have considerably advanced in recent years. In orthopaedic surgery, scaffolds have been used as grafts in procedures involving tendon and ligament reconstruction. This paper aimed to produce and evaluate decellularized tendon scaffolds (DTSs) from biomechanical, microscopic, macroscopic and in vivo perspectives. Read More

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October 2020

Multicenter International Survey on Cardiopulmonary Bypass Perfusion Practices in Adult Cardiac Surgery.

J Cardiothorac Vasc Anesth 2021 Apr 24;35(4):1115-1124. Epub 2020 Aug 24.

King's College Hospital NHS Foundation Trust, London, United Kingdom; King's College London British Heart Foundation Centre of Excellence, London, United Kingdom. Electronic address:

Objectives: To assess current practice in adult cardiac surgery during cardiopulmonary bypass (CPB) across European and non-European countries.

Design: International, multicenter, web-based survey including 28 multiple choice questions addressing hemodynamic and tissue oxygenation parameters, organ protection measures, and the monitoring and usage of anesthetic drugs as part of the anesthetic and perfusion practice during CPB.

Setting: Online survey endorsed by the European Association of Cardiothoracic Anesthesiologists. Read More

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A systematic mutational analysis identifies a 5-residue proline tag that enhances the in vivo immunogenicity of a non-immunogenic model protein.

FEBS Open Bio 2020 Oct 30;10(10):1947-1956. Epub 2020 Aug 30.

Department of Biotechnology and Life Sciences, Graduate School of Engineering, Tokyo University of Agriculture and Technology, Tokyo, Japan.

Poor immunogenicity of small proteins is a major hurdle in developing vaccines or producing antibodies for biopharmaceutical usage. Here, we systematically analyzed the effects of 10 solubility controlling peptide tags (SCP-tags) on the immunogenicity of a non-immunogenic model protein, bovine pancreatic trypsin inhibitor (BPTI-19A; 6 kDa). CD, fluorescence, DLS, SLS, and AUC measurements indicated that the SCP-tags did not change the secondary structure content nor the tertiary structures of the protein nor its monomeric state. Read More

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October 2020

In vitro effect of a novel protease inhibitor cocktail on Toxoplasma gondii tachyzoites.

Exp Parasitol 2020 Dec 29;219:108010. Epub 2020 Sep 29.

Department of Medical Parasitology, Faculty of Medicine, Alexandria University, Egypt. Electronic address:

Toxoplasmosis is a zoonotic disease and a global food and water-borne infection. The disease is caused by the parasite Toxoplasma gondii, which is a highly successful and remarkable pathogen because of its ability to infect almost any nucleated cell in warm-blooded animals. The present study was done to demonstrate the effect of protease inhibitors cocktail (PIC), which inhibit both cysteine and serine proteases, on in vitro cultured T. Read More

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December 2020

Targeting Host Cell Proteases to Prevent SARS-CoV-2 Invasion.

Curr Drug Targets 2021;22(2):192-201

Department of Physiology and Pharmacology, Sapienza University of Rome, Rome, India.

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has spread worldwide and caused widespread devastation. In the absence of definitive therapy, symptomatic management remains the standard of care. Repurposing of many existing drugs, including several anti-viral drugs, is being attempted to tackle the COVID-19 pandemic. Read More

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Proteasuria in nephrotic syndrome-quantification and proteomic profiling.

J Proteomics 2021 01 12;230:103981. Epub 2020 Sep 12.

Department of Internal Medicine, Division of Endocrinology, Diabetology and Nephrology, University Hospital Tübingen, Germany; Institute of Diabetes Research and Metabolic Diseases (IDM), Helmholtz Center Munich, University Tübingen, Germany; German Center for Diabetes Research (DZD), University Tübingen, Germany. Electronic address:

Nephrotic syndrome is characterized by urinary excretion of plasma proteases or proteasuria. There is a lack of data on the quantity, activity status and identity of these aberrantly filtered proteases. We established a fluorescence-based substrate assay to quantify protease activity in urine samples from healthy and nephrotic humans and mice. Read More

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January 2021

Reversible Oligomerization and Reverse Hydrophobic Effect Induced by Isoleucine Tags Attached at the C-Terminus of a Simplified BPTI Variant.

Biochemistry 2020 10 22;59(39):3660-3668. Epub 2020 Sep 22.

Department of Bioengineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan.

Protein amorphous aggregation has become the focus of great attention, as it can impair the ability of cells to function properly. Here, we evaluated the effects of three peptide tags, consisting of one, three, and five consecutive isoleucines attached at the C-terminus end of a simplified bovine pancreatic trypsin inhibitor (BPTI) variant, BPTI-19A, on the thermal stability and oligomerization by circular dichroism spectrometry and differential scanning calorimetry in detail. All of the BPTI-19A variants exhibited a reversible and apparently two-state thermal transition like BPTI-19A at pH 4. Read More

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October 2020

Sapphire-Based Clustering.

J Chem Theory Comput 2020 Oct 24;16(10):6383-6396. Epub 2020 Sep 24.

Biochemistry Department, University of Zurich, Zurich CH-8057, Switzerland.

Molecular dynamics simulations are a popular means to study biomolecules, but it is often difficult to gain insights from the trajectories due to their large size, in both time and number of features. The Sapphire (States And Pathways Projected with HIgh REsolution) plot allows a direct visual inference of the dominant states visited by high-dimensional systems and how they are interconnected in time. Here, we extend this visual inference into a clustering algorithm. Read More

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October 2020

Crystal structures of full length DENV4 NS2B-NS3 reveal the dynamic interaction between NS2B and NS3.

Antiviral Res 2020 10 5;182:104900. Epub 2020 Aug 5.

Lee Kong Chian School of Medicine, Nanyang Technological University, EMB 03-07, 59 Nanyang Drive, 636921, Singapore; NTU Institute of Structural Biology, Nanyang Technological University, EMB 06-01, 59 Nanyang Drive, 636921, Singapore; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, 636921, Singapore. Electronic address:

Flavivirus is a genus of the Flaviviridae family which includes significant emerging and re-emerging human disease-causing arboviruses such as dengue and Zika viruses. Flaviviral non-structural protein 3 (NS3) protease-helicase plays essential roles in viral replication and is an attractive antiviral target. A construct which connects the cytoplasmic cofactor region of NS2B and NS3 protease with an artificial glycine-rich flexible linker has been widely used for structural, biochemical and drug-screening studies. Read More

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October 2020

Protein-Protein Binding as a Two-Step Mechanism: Preselection of Encounter Poses during the Binding of BPTI and Trypsin.

Biophys J 2020 08 10;119(3):652-666. Epub 2020 Jul 10.

Institute of General, Inorganic and Theoretical Chemistry, University of Innsbruck, Innsbruck, Austria. Electronic address:

Biomolecular recognition between proteins follows complex mechanisms, the understanding of which can substantially advance drug discovery efforts. Here, we track each step of the binding process in atomistic detail with molecular dynamics simulations using trypsin and its inhibitor bovine pancreatic trypsin inhibitor (BPTI) as a model system. We use umbrella sampling to cover a range of unbinding pathways. Read More

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A role for mast cells and mast cell tryptase in driving neutrophil recruitment in LPS-induced lung inflammation via protease-activated receptor 2 in mice.

Inflamm Res 2020 Oct 6;69(10):1059-1070. Epub 2020 Jul 6.

Laboratory of Pain and Inflammation, Department of Pharmacology, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Av. Presidente Antônio Carlos, 6627, Pampulha, Belo Horizonte, Minas Gerais, 31270-010, Brazil.

Objective: This study aims to investigate the role of protease-activated receptor (PAR) 2 and mast cell (MC) tryptase in LPS-induced lung inflammation and neutrophil recruitment in the lungs of C57BL/6 mice.

Methods: C57BL/6 mice were pretreated with the PAR2 antagonist ENMD-1068, compound 48/80 or aprotinin prior to intranasal instillation of MC tryptase or LPS. Blood leukocytes, C-X-C motif chemokine ligand (CXCL) 1 production leukocytes recovered from bronchoalveolar lavage fluid (BALF), and histopathological analysis of the lung were evaluated 4 h later. Read More

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October 2020

Expression of ectopic trypsin in atherosclerotic plaques and the effects of aprotinin on plaque stability.

Arch Biochem Biophys 2020 09 27;690:108460. Epub 2020 Jun 27.

Department of Cardiology, Nantong Third People's Hospital, Nantong, Jiangsu, 226001, China.

Background: Our previous research revealed that trypsin is abundantly expressed in atherosclerotic plaques and its distribution overlaps with that of matrix metalloproteinase-9 (MMP-9). This study was performed to explore the possible roles of trypsin in vulnerable atherosclerotic plaque formation.

Methods And Results: Twenty-four rabbits were randomly assigned to a normal (control) group, an atherosclerosis (experimental) group and a trypsin inhibitor (aprotinin) group. Read More

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September 2020