292,865 results match your criteria bone marrow

An enhanced periosteum structure/function dual mimicking membrane for in-situ restorations of periosteum and bone.

Biofabrication 2021 Apr 20. Epub 2021 Apr 20.

Key Laboratory of Textile Science and Technology of Ministry of Education and College of Textiles, Donghua University, 2999 Renmin North Road, Songjiang District, Shanghai, Shanghai, 201620, CHINA.

Periosteum plays a pivotal role in bone formation and reconstruction. The ideal repair process for critical-size bone defects with periosteum damage is to induce regeneration of periosteum tissue and the subsequent bone regeneration derived by the periosteum. Inspired by the bilayer structure of the natural periosteum, we develop a periosteum structure/function dual mimicking membrane for the in-situ restoration of periosteum and bone tissue. Read More

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Quantifying the bone marrow composition of the healthy adult wrist with dual-energy CT.

Eur J Radiol 2021 Apr 16;139:109725. Epub 2021 Apr 16.

Department of Radiology, Bispebjerg and Frederiksberg Hospital, Bispebjerg Bakke 23, 2400, København, Denmark.

Purpose: Purpose of this study was to investigate Dual-energy CT (DECT) derived virtual non-calcium (VNCa) values for absolute quantification of the bone marrow composition in the wrist.

Materials And Methods: We prospectively included consecutive adult participants and examined their wrists with DECT. Ranges of VNCa and calcium values were measured in the carpal bones, radius and ulna using a semi-automatic method. Read More

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Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia.

Acta Biomater 2021 Apr 17. Epub 2021 Apr 17.

College of Life Sciences, Key Laboratory of Bioactive Materials (Ministry of Education), State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, China. Electronic address:

The therapeutic effectiveness of cell transplantation in treatment of diseases and injuries is often limited by low cell retention, survivability, and engraftment. Extracellular matrix (ECM)-derived scaffolds are capable of controlling cell responses, thereby offering potential solutions to current challenges associated with cell therapy. However, it remains a technical challenge to produce ECM scaffolds with highly interconnected porous structure specifically required for cell transplantation. Read More

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Screening for acute disseminated histoplasmosis in HIV disease using urinary antigen detection enzyme immunoassay: A pilot study in Cameroon.

J Microbiol Methods 2021 Apr 17:106226. Epub 2021 Apr 17.

Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, United Kingdom; The Global Action Fund for Fungal Infections, Genève, Switzerland.

Acute disseminated histoplasmosis (ADH) is an AIDS-defining illness and reported in Cameroon, but there are few data about its incidence. Between June and August 2019, we conducted a descriptive cross-sectional study to screen for histoplasmosis in a population of adults with HIV infection, irrespective of their CD4 T-cell counts, using Histoplasma urine antigen detection enzyme immunoassay (EIA) and histoplasmin skin test. Of the 138 participants screened, 36 (26%) had detectable antigen in urine, using an OD cut off of 0. Read More

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Human hematopoietic microenvironments.

PLoS One 2021 20;16(4):e0250081. Epub 2021 Apr 20.

Department of Clinical Cell Biology, Institute of Regional Health Science, University of Southern Denmark, Lillebaelt Hospital, Vejle, Denmark.

Dormancy of hematopoietic stem cells and formation of progenitors are directed by signals that come from the bone marrow microenvironment. Considerable knowledge has been gained on the murine hematopoietic stem cell microenvironment, while less so on the murine progenitor microenvironment and even less so on these microenvironments in humans. Characterization of these microenvironments is decisive for understanding hematopoiesis and finding new treatment modalities against bone marrow malignancies in the clinic. Read More

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National Institutes of Health Consensus Development Project on Criteria for Clinical Trials in Chronic Graft-versus-Host Disease: I. The 2020 Etiology and Prevention Working Group Report.

Transplant Cell Ther 2021 Mar 2. Epub 2021 Mar 2.

Division of Hematological Malignancies and Cellular Therapy, Department of Medicine, Duke Cancer Institute, Durham, North Carolina. Electronic address:

Preventing chronic graft-versus-host disease (GVHD) remains challenging because the unique cellular and molecular pathways that incite chronic GVHD are poorly understood. One major point of intervention for potential prevention of chronic GVHD occurs at the time of transplantation when acute donor anti-recipient immune responses first set the events in motion that result in chronic GVHD. After transplantation, additional insults causing tissue injury can incite aberrant immune responses and loss of tolerance, further contributing to chronic GVHD. Read More

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Analysis of the expression changes of IL-17+ γδ T cells and Treg cells in bone marrow mesenchymal stem cells targeted therapy for allergic rhinitis.

Eur Rev Med Pharmacol Sci 2021 Apr;25(7):2858-2865

Department of Otorhinolaryngology, Children's Hospital of Chongqing Medical University, Chongqing, China; National Clinical Research Center for Child Health and Diseases, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China; Chongqing Key Laboratory of Pediatrics, Chongqing, China.

Objective: Bone marrow mesenchymal stem cells (BMSCs) have immunomodulatory and therapeutic effects on immune system diseases. This study intends to assess the regulatory effect of BMSC targeted therapy on the IL-17+ γδ T cells and Treg cells in allergic rhinitis (AR).

Materials And Methods: BALB/c mice were sensitized by ovalbumin (OVA), while BMSCs were injected intravenously before sensitization and followed by an analysis of nasal symptoms, inflammation, cytokines, and immunoglobulins. Read More

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IL-1β-pre-conditioned mesenchymal stem/stromal cells' secretome modulates the inflammatory response and aggrecan deposition in intervertebral disc.

Eur Cell Mater 2021 Apr 20;41:431-453. Epub 2021 Apr 20.

I3S - Rua Alfredo Allen 208, 4200-135 Porto,

Mesenchymal stem/stromal cells (MSCs) have been increasingly used in clinical trials for low-back pain (LBP) and intervertebral disc (IVD) degeneration with promising results. Their action mechanisms are not fully understood, but they reduce IVD pro-inflammatory markers in a pro-inflammatory/degenerative IVD microenvironment. In this study the therapeutic potential of the MSC secretome, as an alternative cell-free approach for treating degenerated IVDs, was examined. Read More

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Diffusion-weighted magnetic resonance imaging of mandibular bone marrow: do apparent diffusion coefficient values of the cervical vertebrae and mandible correlate with age?

Oral Radiol 2021 Apr 20. Epub 2021 Apr 20.

Department of Stomatology, School of Dentistry, University of São Paulo, 2227 Lineu Prestes Avenue, São Paulo, SP, 05508-000, Brazil.

Objectives: The objective of this investigation was to assess the correlation between the mandible and cervical vertebrae bone marrow apparent diffusion coefficient (ADC), obtained by diffusion-weighted magnetic resonance imaging (DWI), with age; to verify the correlation between ADC values from the mandible and the cervical vertebrae; to describe and assess the differences between ADC values obtained from DWI examinations of distinct mandible areas as well as cervical vertebrae.

Methods: Thirty imaging examinations with DWI for that included the mandible and C1, C2, C3, and C4 vertebrae in the same examination were included. ADC values were collected from 7 distinct areas in the mandible and the cervical vertebrae. Read More

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The impact of prolonged frozen storage on the preparation quality of bird skins and skeletons in zoological collections.

Naturwissenschaften 2021 Apr 20;108(3):18. Epub 2021 Apr 20.

Department of Vertebrates, Museu de Ciències Naturals de Barcelona - Natural Science Museum of Barcelona, Passeig Picasso s/n, 08003, Barcelona, Catalonia, Spain.

Specimens from zoological collections play a pivotal role in improving scientific knowledge in many natural science disciplines. To guarantee an optimum state of conservation and ensure their usefulness, the preparation process employed is crucial. Skins and skeletons are key elements in vertebrate scientific collections and, ideally, are prepared from recently deceased animals; however, specimens are often stored in a frozen state for a long time (years) prior to preparation. Read More

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Transplant-associated thrombotic microangiopathy in pediatric patients: pre-HSCT risk stratification and prophylaxis.

Blood Adv 2021 Apr;5(8):2106-2114

Division of Pediatric Allergy, Immunology, and Bone Marrow Transplant.

Transplant-associated thrombotic microangiopathy (TA-TMA) is an endothelial injury syndrome that complicates hematopoietic stem cell transplant (HSCT). Morbidity and mortality from TA-TMA remain high, making prevention critical. We describe our retrospective single-center experience of TA-TMA after pediatric allogeneic HSCT and present a novel pre-HSCT risk-stratification system and prophylaxis regimen. Read More

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Minimally Invasive Necrotic Bone Washing Improves Bone Healing After Femoral Head Ischemic Osteonecrosis: An Experimental Investigation in Immature Pigs.

J Bone Joint Surg Am 2021 Mar 11. Epub 2021 Mar 11.

Center for Excellence in Hip, Scottish Rite for Children, Dallas, Texas.

Background: Ischemic osteonecrosis of the femoral head produces necrotic cell debris and inflammatory molecules in the marrow space, which elicit a chronic inflammatory repair response. The purpose of this study was to determine the effects of flushing out the necrotic cell debris and inflammatory proteins on bone repair in a piglet model of ischemic osteonecrosis.

Methods: Osteonecrosis of the femoral head of the right hindlimb was induced in 12 piglets by tying a ligature tightly around the femoral neck. Read More

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DECT in Detection of Vertebral Fracture-associated Bone Marrow Edema: A Systematic Review and Meta-Analysis with Emphasis on Technical and Imaging Interpretation Parameters.

Radiology 2021 Apr 20:203624. Epub 2021 Apr 20.

From the Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University, 601 N Caroline St, JHOC 5165, Baltimore, MD 21287 (F.G.S., H.I.S., S.D.); Department of Radiology and Biomedical Imaging, Yale University School of Medicine, New Haven, Conn (D.S., M.H.J.); Department of Radiology, NYU Grossman School of Medicine, New York, NY (J.F.); and Department of Neuroradiology, School of Medicine, Technical University of Munich, Munich, Germany (B.J.S.).

Background Dual-energy CT (DECT) shows promising performance in detecting bone marrow edema (BME) associated with vertebral body fractures. However, the optimal technical and image interpretation parameters are not well described. Purpose To conduct a systematic review and meta-analysis to determine the diagnostic performance of DECT in detecting BME associated with vertebral fractures (VFs), using different technical and image interpretation parameters, compared with MRI as the reference standard. Read More

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How much imatinib is enough?

David M Ross

Br J Haematol 2021 Apr 19. Epub 2021 Apr 19.

Department of Haematology and Bone Marrow Transplantation, Royal Adelaide Hospital, Adelaide, Australia.

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Chromatin remodeler CHD8 governs hematopoietic stem/progenitor survival by regulating ATM-mediated P53 protein stability.

Blood 2021 Apr 19. Epub 2021 Apr 19.

Children's Hospital Research Foundation, Cincinnati, Ohio, United States.

The Chd8 gene encodes a member of chromodomain helicase DNA-binding (CHD) family of SNF2H-like ATP-dependent chromatin remodeler, mutations of which define a subtype of Autism spectrum disorders. Increasing evidence from recent studies indicates that ATP-dependent chromatin-remodeling genes are involved in the control of crucial gene-expression programs in hematopoietic stem/progenitor cell (HSPC) regulation. Here we identify CHD8 as a specific and essential regulator of normal hematopoiesis. Read More

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14q32 rearrangements deregulating BCL11B mark a distinct subgroup of T and myeloid immature acute leukemia.

Blood 2021 Apr 19. Epub 2021 Apr 19.

University of Perugia, Perugia, Italy.

Acute leukemias (AL) of ambiguous lineage are a heterogeneous group of high-risk leukemias characterized by co-expression of myeloid and lymphoid markers. In this study, we identified a distinct subgroup of immature acute leukemias characterized by a broadly variable phenotype, covering acute myeloid leukemia (AML M0 or M1), T/myeloid mixed phenotype acute leukemia (T/M MPAL), and early T-cell precursor acute lymphoblastic leukemia (ETP-ALL). Rearrangements at 14q32/BCL11B are the cytogenetic hallmark of this entity. Read More

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A comprehensive transcriptome signature of murine hematopoietic stem cell aging.

Blood 2021 Apr 19. Epub 2021 Apr 19.

UMCG / University of Groningen, Groningen, Netherlands.

We surveyed 16 published and unpublished data sets to determine whether a consistent pattern of transcriptional deregulation in aging murine hematopoietic stem cells (HSC) exists. Despite substantial heterogeneity between individual studies, we uncovered a core and robust HSC aging signature. We detected increased transcriptional activation in aged HSCs, further confirmed by chromatin accessibility analysis. Read More

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Novel approaches to target the microenvironment of bone metastasis.

Nat Rev Clin Oncol 2021 Apr 19. Epub 2021 Apr 19.

Department of Tumor Biology, Center of Experimental Medicine, University Cancer Center Hamburg (UCCH), University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Bone metastases are a frequent and severe complication of advanced-stage cancers. Breast and prostate cancers, the most common malignancies in women and men, respectively, have a particularly high propensity to metastasize to bone. Conceptually, circulating tumour cells (CTCs) in the bloodstream and disseminated tumour cells (DTCs) in the bone marrow provide a snapshot of the dissemination and colonization process en route to clinically apparent bone metastases. Read More

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Correction to: PD1 inhibitor in combination with 5-azacytidine and low-dose DLI for the successful treatment of AML patients who relapsed after transplantation.

Bone Marrow Transplant 2021 Apr 19. Epub 2021 Apr 19.

National Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.

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Severe acute graft-versus-host disease increases the incidence of blood stream infection and mortality after allogeneic hematopoietic cell transplantation: Japanese transplant registry study.

Bone Marrow Transplant 2021 Apr 19. Epub 2021 Apr 19.

Hematopoietic Stem Cell Transplantation Division, National Cancer Center Hospital, Tokyo, Japan.

This study aimed to clarify the risk factors and prognosis associated with blood stream infection (BSI) in allogeneic hematopoietic cell transplantation (allo-HCT), and the relationship between BSI and acute graft-versus-host disease (aGVHD). This retrospective analysis included 11,098 patients in the Japanese national transplant registry. A total of 2172 patients developed BSI after allo-HCT, with 2332 identified pathogens. Read More

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Transplant-associated thrombotic microangiopathy: theoretical considerations and a practical approach to an unrefined diagnosis.

Bone Marrow Transplant 2021 Apr 19. Epub 2021 Apr 19.

Levine Cancer Institute, Atrium Health, Charlotte, NC, USA.

Transplant-associated thrombotic microangiopathy (TA-TMA) is an increasingly recognized complication of hematopoietic stem cell transplant (HSCT) with high morbidity and mortality. The triad of endothelial cell activation, complement dysregulation, and microvascular hemolytic anemia has the potential to cause end organ dysfunction, multiple organ dysfunction syndrome and death, but clinical features mimic other disorders following HSCT, delaying diagnosis. Recent advances have implicated complement as a major contributor and the therapeutic potential of complement inhibition has been explored. Read More

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Stoss therapy is safe for treatment of vitamin D deficiency in pediatric patients undergoing HSCT.

Bone Marrow Transplant 2021 Apr 19. Epub 2021 Apr 19.

Phoenix Children's Hospital, Phoenix, AZ, USA.

Vitamin D deficiency remains common among pediatric patients undergoing hematopoietic stem cell transplant (HSCT) despite both aggressive and standard of care strategies. This study examined the safety and efficacy of single high-dose oral vitamin D therapy (Stoss therapy) for treatment of vitamin D deficiency in HSCT recipients. Patients ages 1-21 years presenting for HSCT were randomized to receive either Stoss regimen plus weekly/daily supplementation or standard of care, per US Endocrine Society guidelines. Read More

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Changes in Stem Cell Transplant activity and procedures during SARS-CoV2 pandemic in Italy: an Italian Bone Marrow Transplant Group (GITMO) nationwide analysis (TransCOVID-19 Survey).

Bone Marrow Transplant 2021 Apr 19. Epub 2021 Apr 19.

San Raffaele Scientific Institute, Milan, Italy.

The Transplant Centers belonging to Gruppo Italiano per il Trapianto di Midollo Osseo (GITMO) conducted a survey with the aim of evaluating the effect of SARS-CoV2 pandemic on the allogeneic transplant activity in Italy. The pandemic period from 1/3/2020 to 31/7/2020 was compared with the same period in 2019. Overall, in 2020 there was a 2. Read More

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Ubiquitin specific peptidase Usp53 regulates osteoblast versus adipocyte lineage commitment.

Sci Rep 2021 Apr 19;11(1):8418. Epub 2021 Apr 19.

Research Centre, Shriners Hospital for Children - Canada, 1003 Decarie Boulevard, Montreal, QC, H4A 0A9, Canada.

We have previously shown that parathyroid hormone (PTH) induces the phosphorylation of the DNA-binding protein Nascent polypeptide associated complex And Coregulator alpha (NACA), leading to nuclear translocation of NACA and activation of target genes. Using ChIP-Seq against NACA in parallel with RNA-sequencing, we report the identification of Ubiquitin Specific Peptidase 53 (Usp53) as a target gene of PTH-activated NACA in osteoblasts. A binding site for NACA within the ChIP fragment from the Usp53 promoter was confirmed by electrophoretic mobility shift assay. Read More

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Selenomethionine protects hematopoietic stem/progenitor cells against cobalt nanoparticles by stimulating antioxidant actions and DNA repair functions.

Aging (Albany NY) 2021 Apr 19;13. Epub 2021 Apr 19.

Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.

Hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) can differentiate into all blood lineages to maintain hematopoiesis, wound healing, and immune functions. Recently, cobalt-chromium alloy casting implants have been used extensively in total hip replacements; however, cobalt nanoparticles (CoNPs) released from the alloy were toxic to HSCs and HPCs. We aimed to investigate the mechanism underlying the toxic effect of CoNPs on HSCs/HPCs and to determine the protective effect of selenomethionine (SeMet) against CoNPs and . Read More

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GPR182 is an endothelium-specific atypical chemokine receptor that maintains hematopoietic stem cell homeostasis.

Proc Natl Acad Sci U S A 2021 Apr;118(17)

Department of Pharmacology, Max Planck Institute for Heart and Lung Research, Bad Nauheim, 61231, Germany;

G protein-coupled receptor 182 (GPR182) has been shown to be expressed in endothelial cells; however, its ligand and physiological role has remained elusive. We found GPR182 to be expressed in microvascular and lymphatic endothelial cells of most organs and to bind with nanomolar affinity the chemokines CXCL10, CXCL12, and CXCL13. In contrast to conventional chemokine receptors, binding of chemokines to GPR182 did not induce typical downstream signaling processes, including G- and G-mediated signaling or β-arrestin recruitment. Read More

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Systemic light-chain amyloidosis incidentally diagnosed after subtotal parathyroidectomy and thyroid lobectomy.

BMJ Case Rep 2021 Apr 19;14(4). Epub 2021 Apr 19.

Division of Endocrinology, Diabetes, and Metabolism, VA Greater Los Angeles Healthcare System, Los Angeles, California, USA.

A 74-year-old woman with a history of primary hyperparathyroidism, thyroid nodules, atrial fibrillation and pacemaker placement for sick sinus syndrome presented with fatigue, constipation and persistent lower extremity oedema. She underwent subtotal parathyroidectomy and left thyroid lobectomy. Histopathology revealed amyloidosis affecting the thyroidand parathyroids confirmed by Congo Red Staining with Mayo Clinic subtyping of light chain kappa-type amyloidosis. Read More

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