156,029 results match your criteria bone grafting

Single-Cell Analysis of Ploidy and Transcriptome Reveals Functional and Spatial Divergency in Murine Megakaryopoiesis.

Blood 2021 Jun 11. Epub 2021 Jun 11.

Beijing Institute of Genomics, Chinese Academy of Sciences, China National Center for Bioinformation, Beijing, China.

Megakaryocytes (MKs), the platelet progenitor cell, play important roles in hematopoietic stem cell (HSC) maintenance and immunity. However, it is not known whether these diverse programs are executed by a single population or by distinct subsets of cells. Here, we manually-isolated primary CD41+ MKs from the bone marrow (BM) of mice and human donors based on ploidy (2N-32N), performed single-cell RNA sequencing analysis. Read More

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Recent Trends in Multipotent Human Mesenchymal Stem/Stromal Cells: Learning from History and Advancing Clinical Applications.

Kevin Dzobo

OMICS 2021 Jun 25;25(6):342-357. Epub 2021 May 25.

International Center for Genetic Engineering and Biotechnology (ICGEB), Cape Town Component, Cape Town, South Africa.

Early cell biology reports demonstrated the presence of cells with stem-like properties in bone marrow, with both hematopoietic and mesenchymal lineages. Over the years, various investigations have purified and characterized mesenchymal stromal/stem cells (MSCs) from different human tissues as cells with multilineage differentiation potential under the appropriate conditions. Due to their appealing characteristics and versatile potentials, MSCs are leveraged in many applications in medicine such as oncology, bioprinting, and as recent as therapeutics discovery and innovation for COVID-19. Read More

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Treatment of High-Energy Intra-Articular Fractures of Tibia with Ilizarov External Fixator in Adults: A Tertiary Center Experience.

Int J Clin Pract 2021 Jun 11:e14488. Epub 2021 Jun 11.

Dow University of Health Sciences, Karachi, Pakistan.

Objective: To evaluate the clinical and radiological outcomes of the Ilizarov fixator in the proximal and distal fractures of the tibia.

Materials And Methodology: This retrospective study reviewed 59 patients having high-energy intra-articular proximal and distal tibia fractures associated with severe soft-tissue injury, who were managed surgically with an Ilizarov fixator. The functional outcome was evaluated by using the American Orthopaedic Foot and Ankle Society scoring system (AOFAS) and the Association for the Study and Application of the Method of Ilizarov (ASAMI) Italy scoring system for tibial plateau and plafond fractures respectively. Read More

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Oral mucositis in pediatric cancer patients undergoing allogenic hematopoietic stem cell transplantation preventively treated with professional dental care and photobiomodulation: Incidence and risk factors.

Int J Paediatr Dent 2021 Jun 11. Epub 2021 Jun 11.

Instituto de Ensino e Pesquisa, Hospital Sírio-Libanês, São Paulo, Brazil.

Background: Oral mucositis (OM) is an important side effect related to allogeneic hematopoietic stem cell transplantation (allo-HSCT) and it has been associated with a significative reduction of quality of life. A negative impact of this toxicity in pediatric patients could result in an increased use of parenteral feeding and opioids, longer periods of hospitalization and higher risk of systemic infection.

Aim: To investigate the clinical features, and clinical outcomes associated with OM development and severity in hematologic cancer pediatric patients undergoing allo-HSCT who underwent professional dental care (PDC) and photobiomodulation (PBM) as prophylactic treatment. Read More

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Combination of a Hydraulic Device and Nanohydroxylapatite Paste for Minimally Invasive Transcrestal Sinus Floor Elevation: Procedure and 4-Year Results.

Int J Oral Maxillofac Implants 2021 May-Jun;36(3):587-597

Purpose: The objectives of this retrospective study were to describe a transcrestal sinus floor elevation technique combining the use of a hydraulic device and a nanohydroxylapatite paste and to report on 4-year clinical and radiographic outcomes.

Materials And Methods: The sinus floor elevation procedure used a specially designed drill (SinusJet) to start sinus membrane unsticking and a nanohydroxylapatite paste (Ostim) for further sinus membrane elevation and bone augmentation. It was performed as a one-step procedure with immediate implant placement or a two-step procedure with delayed implant placement 9 months later. Read More

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Clinical and Radiographic Evaluation of Modified Transalveolar Two-Step Osteotome-Mediated Localized Maxillary Sinus Elevation: A Retrospective Computed Tomography Study with a 3-Year Follow-up.

Int J Oral Maxillofac Implants 2021 May-Jun;36(3):553-560

Purpose: This study aimed to report a practicable and noninvasive two-stage technique for sinus elevation and delayed implant insertion in the augmented site with residual bone height down to 3 mm or even lower.

Materials And Methods: The surgical technique employed a two-stage process for rehabilitation of posterior maxillary single-tooth edentulous areas, involving, in the first step, transcrestal maxillary sinus floor augmentation with a collagen sponge to fill the intrabony cavity resulting from the detachment of the sinus membrane; the second step consisted of another indirect sinus floor elevation using magnetoelectric surgery with immediate implant placement and no grafting material. Changes in bone height were evaluated by a comparison of the computed tomography scans acquired before treatment and after surgery (at 3 months and 5 years of the survey). Read More

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A Comparison of the Autogenous Bone Collection Capacity of Two Bur Drill Systems Used in Implant Surgery with Different Bur Diameters and Lengths at Different Drilling Speeds: An In Vitro Study.

Int J Oral Maxillofac Implants 2021 May-Jun;36(3):468-473

Purpose: The aim of this study was to compare the bone collection capacity of bur drill systems used in implant surgery with different diameters, lengths, and drilling speeds.

Materials And Methods: This study was performed on bovine ribs. Two bur drill systems were studied: Implantium (Dentium) and Straumann (Institut Straumann). Read More

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Magnetic Resonance Imaging for the Planning, Execution, and Follow-up of Implant-Based Oral Rehabilitation: Systematic Review.

Int J Oral Maxillofac Implants 2021 May-Jun;36(3):432-441

Purpose: To undertake a systematic literature review of magnetic resonance imaging (MRI) employed in the three phases of implant-based oral rehabilitation: planning, execution, and follow-up.

Materials And Methods: MEDLINE (PubMed) and EMBASE bibliographic databases were searched up to January 2020 for studies assessing the use of MRI alone or in connection with CT and/or CBCT in the planning, execution, or follow-up of dental implant placement and/or bone grafting procedures in the maxilla or the mandible. Included studies were also assessed according to the diagnostic imaging efficacy scale presented by Fryback and Thornbury (F&T). Read More

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Comparative in vitro study of commercially available products for alveolar ridge preservation.

J Periodontol 2021 Jun 11. Epub 2021 Jun 11.

Naval Medical Research Unit San Antonio, JBSA Fort Sam Houston, Texas.

Background: Ridge preservation is performed by placing a biocompatible product, following tooth extraction, to maintain bone volume. However, current ridge preservation therapies do not always maintain the volume required for future implant placement. Variations in surgical technique and material selection contribute to determining clinical outcomes. Read More

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Characteristics of Patients ≥ 10 Years of Age with Diffuse Intrinsic Pontine Glioma: A Report from the International DIPG Registry.

Neuro Oncol 2021 Jun 11. Epub 2021 Jun 11.

The Ohio State University College of Medicine, Columbus, OH.

Background: DIPG generally occurs in young school-age children, although can occur in adolescents and young adults. The purpose of this study was to describe clinical, radiological, pathologic, and molecular characteristics in patients ≥10 years of age with DIPG enrolled in the International DIPG Registry (IDIPGR).

Methods: Patients ≥10 years of age at diagnosis enrolled in the IDIPGR with imaging confirmed DIPG diagnosis were included. Read More

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A retrospective preliminary histomorphometric and clinical investigation on sinus augmentation using enzyme-deantigenic, collagen-preserving equine bone granules and plasma rich in growth factors.

Int J Implant Dent 2021 Jun 11;7(1):60. Epub 2021 Jun 11.

Dental School, Vita-Salute University IRCCS San Raffaele, Milan, Italy.

Background: Enzyme-deantigenic equine bone (EDEB) is a substitute of autogenous bone. Mixing it with plasma rich in growth factors (PRGF) seems a viable option to achieve enhanced bone formation in alveolar bone augmentation surgeries. This retrospective study aims to first report the histomorphometric and clinical outcomes achieved when using the EDEB/PRGF mixture for performing sinus augmentation procedures followed by delayed implant placement. Read More

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CD38 deficiency alleviates Ang II-induced vascular remodeling by inhibiting small extracellular vesicle-mediated vascular smooth muscle cell senescence in mice.

Signal Transduct Target Ther 2021 Jun 11;6(1):223. Epub 2021 Jun 11.

The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University, Nanchang, People's Republic of China.

CD38 is the main enzyme for nicotinamide adenine dinucleotide (NAD) degradation in mammalian cells. Decreased NAD levels are closely related to metabolic syndromes and aging-related diseases. Our study showed that CD38 deficiency significantly alleviated angiotensin II (Ang II)-induced vascular remodeling in mice, as shown by decreased blood pressures; reduced vascular media thickness, media-to-lumen ratio, and collagen deposition; and restored elastin expression. Read More

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Protective Effects of Bone Marrow Mesenchymal Stem Cells (BMMSCS) Combined with Normothermic Machine Perfusion on Liver Grafts Donated After Circulatory Death via Reducing the Ferroptosis of Hepatocytes.

Med Sci Monit 2021 Jun 11;27:e930258. Epub 2021 Jun 11.

Department of Organ Transplantation, Tianjin First Central Hospital, Tianjin, China (mainland).

BACKGROUND To improve the quality of liver grafts from extended-criteria donors donated after circulatory death (DCD), this study explored whether bone marrow mesenchymal stem cells (BMMSCs) combined with normothermic machine perfusion (NMP) have protective effects on DCD donor livers and the effects of ferroptosis in this procedure. MATERIAL AND METHODS Twenty-four male rat DCD donor livers were randomly and averagely divided into normal, static cold storage (SCS), NMP, and NMP combined with BMMSCs groups. Liver function, bile secretion, and pathological features of DCD donor livers were detected to evaluate the protective effects of NMP and BMMSCs on DCD donor livers. Read More

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Pretransplant Short-Term Exposure of Donor Graft Cells to ITK Selective Inhibitor Ameliorates Acute Graft-versus-Host Disease by Inhibiting Effector T Cell Differentiation while Sparing Regulatory T Cells.

Immunohorizons 2021 Jun 10;5(6):424-437. Epub 2021 Jun 10.

Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan

Graft-versus-host disease (GVHD) remains to be a significant cause of morbidity and mortality after allogeneic hematopoietic stem cell transplantation (HSCT). IL-2-inducible T cell kinase (ITK), a TEC cytoplasmic tyrosine kinase, has an essential role in T cell development and receptor signaling. The ITK/Bruton tyrosine kinase inhibitor ibrutinib has been shown to improve chronic GVHD symptoms; however, the effect of ITK selective inhibition on acute GVHD remains unclear. Read More

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Human endometrium-derived stem cell improves cardiac function after myocardial ischemic injury by enhancing angiogenesis and myocardial metabolism.

Stem Cell Res Ther 2021 Jun 10;12(1):344. Epub 2021 Jun 10.

The Laboratory of Stem Cell Regenerative Medicine Research, Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Key Laboratory of Cell Physiology of Ministry of Education, Shanxi Medical University, Taiyuan, China.

Background: The human endometrium in premenopausal women is an active site of physiological angiogenesis, with regenerative cells present, suggesting that the endometrium contains adult angiogenic stem cells. In the context of cardiac repair after ischemic injury, angiogenesis is a crucial process to rescue cardiomyocytes. We therefore investigated whether human endometrium-derived stem cells (hEMSCs) can be used for cardiac repair after ischemic injury and their possible underlying mechanisms. Read More

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Intra-iliac bone marrow injection as a novel alternative to intra-tibial inoculation in rat model.

Stem Cell Res Ther 2021 Jun 10;12(1):336. Epub 2021 Jun 10.

Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.

Background: Intra-bone marrow injection (IBMI) in rats is adopted in many studies for stem cell and hematopoietic cell transplantation. IBMI in the tibia or the femur results in severe distress to the animal. Therefore, this study aims to evaluate intra-iliac injections as an alternative approach for IBMI. Read More

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Rat perichondrium transplanted to articular cartilage defects forms articular-like, hyaline cartilage.

Bone 2021 Jun 7:116035. Epub 2021 Jun 7.

Division of Pediatric Endocrinology and Center for Molecular Medicine, L8:01, Department of Women's and Children's Health, Karolinska Institutet and University Hospital, Stockholm, Sweden; School of Medical Sciences, Örebro University and University Hospital, Örebro, Sweden. Electronic address:

Objective: Perichondrium autotransplants have been used to reconstruct articular surfaces destroyed by infection or trauma. However, the role of the transplanted perichondrium in the healing of resurfaced joints have not been investigated.

Design: Perichondrial and periosteal tissues were harvested from rats hemizygous for a ubiquitously expressed enhanced green fluorescent protein (EGFP) transgene and transplanted into full-thickness articular cartilage defects at the trochlear groove of distal femur in wild-type littermates. Read More

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Infection related mortality in adults and children undergoing allogeneic hematopoietic cell transplantation: An Australian registry report.

Transplant Cell Ther 2021 Jun 7. Epub 2021 Jun 7.

National Centre for Infection in Cancer, Peter MacCallum Cancer Centre, Melbourne, Australia; Department of Infectious Diseases, Peter MacCallum Cancer Centre, Melbourne, Australia; Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Australia.

Background: Infection related mortality (IRM) is the most common non-relapse mortality reported post allogeneic hematopoietic cell transplantation (HCT). Information on the incidence and timing of specific infective organisms and the risk factors for IRM is essential to developing prevention strategies.

Objective: This report provides the first account of IRM in adults and children undergoing HCT in Australia. Read More

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Therapeutic options for CTLA-4 Insufficiency.

J Allergy Clin Immunol 2021 Jun 7. Epub 2021 Jun 7.

The Children's Hospital of Philadelphia, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Background: Heterozygous germline mutations in cytotoxic T-lymphocyte-associated antigen-4 (CTLA4) impair the immunomodulatory function of regulatory T cells. Affected individuals are prone to life-threatening autoimmune and lymphoproliferative complications. A number of therapeutic options are currently used with variable effectiveness. Read More

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The Effect of Neutropenia and Filgrastim (G-CSF) in Cancer Patients With COVID-19 Infection.

Clin Infect Dis 2021 Jun 10. Epub 2021 Jun 10.

Infectious Disease, Department of Medicine, Memorial Sloan Kettering Cancer Center, NY, NY, USA.

Background: Neutropenia is commonly encountered in cancer patients. Recombinant human granulocyte colony-stimulating factor (G-CSF, filgrastim), a cytokine that initiates proliferation and differentiation of mature granulocytes, is widely given to oncology patients to counteract neutropenia, reducing susceptibility to infection. However, the clinical impact of neutropenia and G-CSF use in cancer patients with COVID-19 remains unknown. Read More

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Investigation into the effects of leukemia inhibitory factor on the bone repair capacity of BMSCs-loaded BCP scaffolds in the mouse calvarial bone defect model.

J Bioenerg Biomembr 2021 Jun 10. Epub 2021 Jun 10.

Department of Stomatology, Southern University of Science and Technology Yantian Hospital, Shenzhen, China.

Leukemia inhibitory factor (LIF) is known to play a major role in bone physiology. In the present study, we examined the in vitro effects of LIF on osteoblast differentiation of bone marrow stem cells (BMSCs) and explored in vivo effects of LIF on the bone repair capacity of BMSCs-loaded biphasic calcium phosphate (BCP) scaffolds in mouse calvarial bone defect model. The mRNA and protein expression levels in the BMSCs were determined by quantitative real-time PCR and western blot, respectively; the in vitro osteoblast differentiation of the BMSCs was evaluated by using Alizarin Red S staining. Read More

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Patients with hematologic cancers are more vulnerable to COVID-19 compared to patients with solid cancers.

Intern Emerg Med 2021 Jun 10. Epub 2021 Jun 10.

Deputy Minister of Health, Republic of Turkey, Ankara, Turkey.

Previous studies reported that COVID-19 patients with cancer had higher rates of severe events such as intensive care unit (ICU) admission, mechanical ventilation (MV) assistance, and death during the COVID-19 course compared to the general population. However, no randomized study compared the clinical course of COVID-19 in patients with hematologic cancers to patients with solid cancers. Thus, in this study, we intend to reveal the outcome of COVID-19 in hematologic cancer patients and compare their outcomes with COVID-19 patients with solid cancers. Read More

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BCL-2 Inhibition as Treatment for Chronic Lymphocytic Leukemia.

Curr Treat Options Oncol 2021 Jun 10;22(8):66. Epub 2021 Jun 10.

Bone Marrow Transplantation Unit, Hospital Israelita Albert Einstein, Av. Albert Einstein (SP), São Paulo, 627/520, Brazil.

Opinion Statement: At the end of the 1990s, with the advent of imatinib for chronic myeloid leukemia and rituximab for B cell lymphoproliferative diseases with CD20 expression, there was a great conceptual evolution in the treatment of onco-hematological diseases. Researchers from around the world and the pharmaceutical industry began to focus their efforts on the so-called target therapy used alone or associated with classic chemotherapeutic drugs. In chronic lymphocytic leukemia, the development of second-generation anti-CD20 antibodies, biosimilars, PI3K (phosphatidylinositol 3-kinases) inhibitors, BTK (Bruton's tyrosine kinase) inhibitors, and anti-bcl 2 drugs represented mainly by venetoclax brought new, broader, and more effective opportunities in the treatment of this disease. Read More

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Extended loci histocompatibility matching in HSCT-Going beyond classical HLA.

Int J Immunogenet 2021 Jun 9. Epub 2021 Jun 9.

ZKRD - Zentrales Knochenmarkspender-Register für Deutschland, German National Bone Marrow Donor Registry, Ulm, Germany.

Unrelated haematopoietic stem cell transplantation (HSCT) has evolved from an experimental protocol to a potentially curative first-line treatment in a variety of haematologic malignancies. The continuous refinement of treatment protocols and supportive care paired with ongoing achievements in the technological field of histocompatibility testing enabled this transformation. Without a doubt, HLA matching is still the foremost criterion for donor selection in unrelated HSCT. Read More

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[The relationships between different surgical approaches for maxillary sinus augmentation and implant failure and complications: a retrospective cohort study].

Shanghai Kou Qiang Yi Xue 2021 Apr;30(2):214-218

Department of Oral and Maxillofacial Surgery, Hefei Stomatology Hospital. Hefei 230001, Anhui Province, China.

Purpose: To investigate the 5-year survival and complication rates of implants placed in grafted sinuses with different surgical approaches and analyze the causes for failure.

Methods: This study retrospectively observed the prognosis of patients who underwent maxillary sinus augmentation by means of lateral window technique(LWT) or transalveolar osteotomy technique (TOT) and simultaneously installed implants performed, in Hefei Stomatological Hospital. The primary predictor variables were surgical approaches, including LWT and TOT. Read More

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Preliminary Report of Arthroscopically Assisted Sauvé-Kapandji Procedure for Distal Radioulnar Joint Arthritis.

J Wrist Surg 2021 Jun 5;10(3):262-267. Epub 2021 Jan 5.

Department of Orthopaedic Surgery, Saiseikai Shimonoseki General Hospital, Shimonoseki, Japan.

 The arthroscopically assisted Sauvé-Kapandji (S-K) procedure has been described as a safe and promising technique for distal radioulnar joint (DRUJ) arthrodesis. Our purpose was to investigate the advantages and disadvantages of the arthroscopically assisted S-K procedure.  Eight patients underwent an arthroscopically assisted S-K procedure. Read More

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Volar Plating versus Headless Compression Screw Fixation of Scaphoid Nonunions: A Meta-analysis of Outcomes.

J Wrist Surg 2021 Jun 3;10(3):255-261. Epub 2021 Jan 3.

Department of Orthopaedic Surgery, The Rothman Institute at Thomas Jefferson University, Philadelphia, Pennsylvania.

 Headless compression screw fixation with bone grafting has been the mainstay of treatment for scaphoid nonunion for the past several decades. Recently, locked volar plate fixation has gained popularity as a technique for scaphoid fixation, especially for recalcitrant or secondary nonunions.  The purpose of this meta-analysis was to compare union rates and clinical outcomes between locked volar plate fixation and headless compression screw fixation for the treatment of scaphoid nonunions. Read More

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Use of high-dose mesna and hyperhydration leads to lower incidence of hemorrhagic cystitis after posttransplant cyclophosphamide-based allogeneic transplantation.

Bone Marrow Transplant 2021 Jun 9. Epub 2021 Jun 9.

Department of Hematology and Hematopoietic Cell Transplantation, City of Hope, Duarte, CA, USA.

Currently, there is no consensus on best practices to prevent hemorrhagic cystitis (HC) in patients receiving posttransplant cyclophosphamide (PTCy). We retrospectively reviewed 194 patients undergoing their first hematopoietic cell transplant (HCT) who received PTCy from 2014 to 2018 to describe the incidence and severity of HC, identify potential risk factors, and impact of HC on HCT outcomes. Standard HC prophylaxis was hyperhydration with forced diuresis and mesna at 320% the daily dose of PTCy. Read More

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Efficacy and cost analysis of eltrombopag in thrombocytopenia and poor graft function post allogeneic hematopoietic cell transplantation.

Bone Marrow Transplant 2021 Jun 9. Epub 2021 Jun 9.

Hans Messner Allogeneic Blood and Marrow Transplant Program, Princess Margaret Cancer Center, Toronto, ON, Canada.

Eltrombopag has shown efficacy in the treatment of thrombocytopenia and poor graft function (PGF) after allogeneic hematopoietic cell transplantation (HCT) in retrospective observational studies, but is not approved for this indication. The cost of this drug is also a major concern in publicly funded health care systems. We collected data about patients who received eltrombopag for thrombocytopenia or PGF after HCT. Read More

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