135 results match your criteria bonds trehalose


Trehalose Restrains the Fibril Load towards α-Lactalbumin Aggregation and Halts Fibrillation in a Concentration-Dependent Manner.

Biomolecules 2021 Mar 11;11(3). Epub 2021 Mar 11.

Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.

Protein aggregation and misfolding are some of the most challenging obstacles, customarily studied for their association with amyloid pathologies. The mechanism of amyloid fibrillation development is a dynamic phenomenon involving various factors such as the intrinsic properties of protein and the physical and chemical environmental conditions. The purpose of this study was to see the thermal aggregation profile of alpha-lactalbumin (α-LA) and to delineate the effect of trehalose on its aggregation profile. Read More

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Improved viability of Lactobacillus plantarum embedded in whey protein concentrate/pullulan/trehalose hydrogel during freeze drying.

Carbohydr Polym 2021 May 20;260:117843. Epub 2021 Feb 20.

College of Food Science and Engineering, Jilin Agricultural University, Changchun, China; Jilin Province Innovation Center for Food Biological Manufacture, Jilin Agricultural University, Changchun, China; National Processing Laboratory for Soybean Industry and Technology, Changchun, China; National Engineering Laboratory for Wheat and Corn Deep Processing, Changchun, China. Electronic address:

In this study, trehalose (TRE) was added to prepare whey protein concentrate (WPC)/pullulan (PUL)/TRE hydrogel and the hydrogel was used as the wall material to improve the viability of encapsulated L. plantarum during freeze drying and storage. The optimum conditions were 5. Read More

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Neutron total scattering investigation on the dissolution mechanism of trehalose in NaOH/urea aqueous solution.

Struct Dyn 2021 Jan 10;8(1):014901. Epub 2021 Feb 10.

Institute for Advanced Study, Shenzhen University, Shenzhen 508060, China.

Trehalose is chosen as a model molecule to investigate the dissolution mechanism of cellulose in NaOH/urea aqueous solution. The combination of neutron total scattering and empirical potential structure refinement yields the most probable all-atom positions in the complex fluid and reveals the cooperative dynamic effects of NaOH, urea, and water molecules in the dissolution process. NaOH directly interacts with glucose rings by breaking the inter- and intra-molecular hydrogen bonding. Read More

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January 2021

Cunninghamella echinulata PA3S12MM invertase: Biochemical characterization of a promiscuous enzyme.

J Food Biochem 2021 Apr 17;45(4):e13654. Epub 2021 Feb 17.

Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, Cascavel, Brazil.

The Cunninghamella echinulata PA3S12MM fungus is a great producer of invertases in a growth medium supplemented by apple peels. The enzyme was purified 4.5 times after two chromatographic processes, and it presented a relative molecular mass of 89. Read More

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An improved strategy of TGFβ3 expression in Escherichia coli: Exploiting folding modulators for a switch from misfolded to folded form.

Int J Biol Macromol 2021 Jan 2;167:787-795. Epub 2020 Dec 2.

Department of Chemistry, Indian Institute of Technology, Delhi, India. Electronic address:

Transforming growth factor beta 3 (TGFβ3) exhibits a complex native structure featuring the presence of multiple disulfide bonds forming the active dimer. Consequently, its heterologous expression in microbial system invariably leads to inclusion body (IB) formation. In this study, we observed an interesting phenomenon of switching a significant fraction of misfolded TGFβ3 to folded form by modulating the cellular protein folding machinery. Read More

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January 2021

Anomeric Selectivity of Trehalose Transferase with Rare l-Sugars.

ACS Catal 2020 Aug 22;10(15):8835-8839. Epub 2020 Jul 22.

Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands.

Retaining LeLoir glycosyltransferases catalyze the formation of glycosidic bonds between nucleotide sugar donors and carbohydrate acceptors. The anomeric selectivity of trehalose transferase from was investigated for both d- and l-glycopyranose acceptors. The enzyme couples a wide range of carbohydrates, yielding trehalose analogues with conversion and enantioselectivity of >98%. Read More

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Stabilization of proteins embedded in sugars and water as studied by dielectric spectroscopy.

Phys Chem Chem Phys 2020 Sep;22(37):21197-21207

Department of Physics, Chalmers University of Technology, SE-412 96 Göteborg, Sweden.

In many products proteins have become an important component, and the long-term properties of these products are directly dependent on the stability of their proteins. To enhance this stability it has become common to add disaccharides in general, and trehalose in particular. However, the mechanisms by which disaccharides stabilize proteins and other biological materials are still not fully understood, and therefore we have here used broadband dielectric spectroscopy to investigate the stabilizing effect of the disaccharides trehalose and sucrose on myoglobin, with the aim to enhance this understanding in general and to obtain specific insights into why trehalose exhibits extraordinary stabilizing properties. Read More

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September 2020

Purification, isolation, and structure characterization of water soluble and insoluble polysaccharides from Maitake fruiting body.

Int J Biol Macromol 2020 Dec 10;164:1879-1888. Epub 2020 Aug 10.

State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business, China; School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. Electronic address:

The crude polysaccharides (GFP) were isolated from the Maitake fruiting body (Grifola frondosa) and purified by DEAE Cellulose-52 ionic-exchange chromatography and Sephadex G-25 gel filtration chromatography in that order. Five main fractions, GFP-1 to GFP-5 were obtained through the isolation and purification steps. Free sugars were isolated by G-25 gel filtration chromatography and identified glucose and (α,α)-trehalose by nuclear magnetic resonance (NMR). Read More

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December 2020

Structural Analysis of Binding Determinants of Trehalose-6-phosphate Phosphatase Using Ground-State Complexes.

Biochemistry 2020 09 17;59(35):3247-3257. Epub 2020 Aug 17.

Department of Chemistry, Boston University, Boston, Massachusetts 02215, United States.

Trehalose-6-phosphate phosphatase (T6PP) catalyzes the dephosphorylation of trehalose 6-phosphate (T6P) to the disaccharide trehalose. The enzyme is not present in mammals but is essential to the viability of multiple lower organisms as trehalose is a critical metabolite, and T6P accumulation is toxic. Hence, T6PP is a target for therapeutics of human pathologies caused by bacteria, fungi, and parasitic nematodes. Read More

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September 2020

Interaction between tau and water during the induced aggregation revealed by near-infrared spectroscopy.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Apr 8;230:118046. Epub 2020 Jan 8.

Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, PR China; Tianjin Key Laboratory of Biosensing and Molecular Recognition, Tianjin 300071, PR China; State Key Laboratory of Medicinal Chemical Biology, Tianjin 300071, PR China. Electronic address:

Near-infrared (NIR) spectra are sensitive to the variation of water structure. In this work, NIR spectroscopy was used to investigate the variation of hydration water during the aggregation of R2/wt induced by heparin. The osmolytes, urea and trehalose, were used to slow down and speed up the aggregation. Read More

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Properties of Aqueous Trehalose Mixtures: Glass Transition and Hydrogen Bonding.

J Chem Theory Comput 2020 Feb 24;16(2):1249-1262. Epub 2020 Jan 24.

Institute of Chemistry, the Fritz Haber Research Center, and the Harvey M. Kruger Center for Nanoscience & Nanotechnology , The Hebrew University , Jerusalem 9190401 , Israel.

Trehalose is a naturally occurring disaccharide known to remarkably stabilize biomacromolecules in the biologically active state. The stabilizing effect is typically observed over a large concentration range and affects many macromolecules including proteins, lipids, and DNA. Of special interest is the transition from aqueous solution to the dense and highly concentrated glassy state of trehalose that has been implicated in bioadaptation of different organisms toward desiccation stress. Read More

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February 2020

Hydration Dynamics in Solutions of Cyclic Polyhydroxyl Osmolytes.

J Phys Chem B 2019 10 1;123(40):8472-8479. Epub 2019 Oct 1.

Department of Physics , Ripon College , Ripon , Wisconsin 54971 , United States.

Simple sugars are remarkably effective at preserving protein and enzymatic structures against thermal and hydrostatic stress. Here, we investigate the hydrodynamic and biopreservative properties of three small cyclic molecules: glucose, -inositol, and methyl-α-d-glucopyranoside using circular dichroism spectroscopy and isothermal calorimetry. Using ultrafast fluorescence frequency upconversion spectroscopy, we measure the dynamical retardation of hydration dynamics in cosolute solutions. Read More

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October 2019

Characterization of hydration water in supercooled water-trehalose solutions: The role of the hydrogen bonds network.

J Chem Phys 2019 Jul;151(4):044507

Dipartimento di Matematica e Fisica, Università Roma Tre, Via della Vasca Navale 84, 00146 Rome, Italy.

The structural and dynamical properties of hydration water in aqueous solutions of trehalose are studied with molecular dynamics simulation. We simulate the systems in the supercooled region to investigate how the interaction with the trehalose molecules modifies the hydrogen bond network, the structural relaxation, and the diffusion properties of hydration water. The analysis is performed by considering the radial distribution functions, the residence time of water molecules in the hydration shell, the two body excess entropy, and the hydrogen bond water-water and water-trehalose correlations of the hydration water. Read More

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Excipient selection for thermally stable enveloped and non-enveloped viral vaccine platforms in dry powders.

Int J Pharm 2019 Apr 28;561:66-73. Epub 2019 Feb 28.

Department of Chemical Engineering, McMaster University, Hamilton, Ontario, Canada. Electronic address:

Two enveloped viral vectors, vesicular stomatitis virus and influenza virus, and a non-enveloped viral vector, human adenovirus type 5, were encapsulated by spray drying to enhance thermal stability.Results with these candidates led to the hypothesis that stability performance of chosen excipients may be less virus-specific, as previously postulated in the literature, and more differentiated based on whether the virus has a lipid envelope. Spray dried samples were characterized for their thermal properties, RNA viability and in vitro viral activity after storage at 37 °C for up to 30 days or at 45 °C for up to 3 days. Read More

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Artificial Fusion of mCherry Enhances Trehalose Transferase Solubility and Stability.

Appl Environ Microbiol 2019 04 4;85(8). Epub 2019 Apr 4.

Biocatalysis, Department of Biotechnology, Delft University of Technology, Delft, The Netherlands

LeLoir glycosyltransferases are important biocatalysts for the production of glycosidic bonds in natural products, chiral building blocks, and pharmaceuticals. Trehalose transferase (TreT) is of particular interest since it catalyzes the stereo- and enantioselective α,α-(1→1) coupling of a nucleotide sugar donor and monosaccharide acceptor for the synthesis of disaccharide derivatives. Heterologously expressed thermophilic trehalose transferases were found to be intrinsically aggregation prone and are mainly expressed as catalytically active inclusion bodies in To disfavor protein aggregation, the thermostable protein mCherry was explored as a fluorescent protein tag. Read More

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Insights into ice-growth inhibition by trehalose and alginate oligosaccharides in peeled Pacific white shrimp (Litopenaeus vannamei) during frozen storage.

Food Chem 2019 Apr 19;278:482-490. Epub 2018 Nov 19.

College of Food Science and Engineering, Qingdao Agriculture University, PR China.

Cryoprotective saccharides are widely accepted antifreeze additives that reduce thawing loss, maintain texture, and retard protein denaturation in frozen seafood. In this study, the inhibition effects of trehalose and alginate oligosaccharides on ice growth were investigated and compared with sodium pyrophosphate (NaPO) treatment in peeled shrimp during frozen storage, especially the interactions between saccharide molecules and ice crystals. The microstructural results demonstrated that the pre-soaking of trehalose and alginate oligosaccharides before freezing exhibited marked effects on stability of muscle tissue structures and slowed the damage caused to the myofibrils by large ice crystals. Read More

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Portulaca elatior root contains a trehalose-binding lectin with antibacterial and antifungal activities.

Int J Biol Macromol 2019 Apr 22;126:291-297. Epub 2018 Dec 22.

Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil. Electronic address:

Lectins are carbohydrate-binding proteins broadly distributed in plants and have several biological functions, including antimicrobial action. Portulaca elatior is a Caatinga plant whose chemical composition and biotechnological potential have not been extensively studied. In this work, a lectin was isolated from P. Read More

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High-efficiency expression of Sulfolobus acidocaldarius maltooligosyl trehalose trehalohydrolase in Escherichia coli through host strain and induction strategy optimization.

Bioprocess Biosyst Eng 2019 Mar 6;42(3):345-354. Epub 2018 Dec 6.

State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.

Maltooligosyl trehalose trehalohydrolase (MTHase, EC 3.2.1. Read More

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Trehalose in Water Revisited.

J Phys Chem B 2018 07 17;122(29):7365-7374. Epub 2018 Jul 17.

Department of Biochemistry , University of Oxford , South Parks Road , Oxford OX1 3QU , U.K.

Trehalose, commonly found in living organisms, is believed to help them survive severe environmental conditions, such as drought or extreme temperatures. With the aim of trying to understand these properties, two recent neutron scattering studies investigate the structure of trehalose water solutions but come to seemingly opposite conclusions. In the first study, which looks at two concentrations of trehalose-water mole ratios of 1:100 and 1:25, the conclusion is that trehalose hydrogen-bonds to water rather weakly and has a relatively minor impact on the structure of water in solution compared to bulk water. Read More

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Unraveling the molecular mechanism of the effects of sodium dodecyl sulfate, salts, and sugars on amyloid fibril formation in camel IgG.

Colloids Surf B Biointerfaces 2018 Oct 19;170:430-437. Epub 2018 Jun 19.

Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.

Sodium dodecyl sulfate (SDS) is an anionic surfactant that can be used to stimulate protein fibrillation in vitro. Here, we investigated the effects of SDS on camel IgG aggregation at pH 3.5 and 7. Read More

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October 2018

Hydrogen Bond Length as a Key To Understanding Sweetness.

J Phys Chem Lett 2018 Jul 22;9(13):3667-3672. Epub 2018 Jun 22.

Dipartimento di Scienze, Sezione di Nanoscienze , Università degli Studi "Roma Tre" , Via della Vasca Navale 84 , 00146 Roma , Italy.

Neutron diffraction experiments have been performed to investigate and compare the structure of the hydration shell of three monosaccharides, namely, fructose, glucose, and mannose. It is found that despite their differences with respect to many thermodynamical quantities, bioprotective properties against environmental stresses, and taste, the influence of these monosaccharides on the bulk water solvent structure is virtually identical. Conversely, these sugars interact with the neighboring water molecules by forming H bonds of different length and strength. Read More

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The effect of light and temperature on the dynamic state of Rhodobacter sphaeroides reaction centers proteins determined from changes in tryptophan fluorescence lifetime and PQ recombination kinetics.

J Photochem Photobiol B 2018 Mar 6;180:140-148. Epub 2018 Feb 6.

Department of Biophysics, Biological Faculty of the M.V. Lomonosov Moscow State University, 119991 Moscow, Russia.

The temperature dependencies of the rate of dark recombination of separated charges between the photoactive bacteriochlorophyll and the primary quinone acceptor (Q) in photosynthetic reaction centers (RCs) of the purple bacteria Rhodobacter sphaeroides (Rb. sphaeroides) were investigated. Measurements were performed in water-glycerol and trehalose environments after freezing to -180 °C in the dark and under actinic light with subsequent heating. Read More

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Functional characterization of Helicoverpa armigera trehalase and investigation of physiological effects caused due to its inhibition by Validamycin A formulation.

Int J Biol Macromol 2018 Jun 26;112:638-647. Epub 2018 Feb 26.

Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Ganeshkhind, Pune 411007, Maharashtra, India. Electronic address:

Trehalase catalyzes hydrolysis of trehalose and plays a crucial role in insect metabolism. In the present study, phylogenetic analysis and multiple sequence alignment suggested that H. armigera trehalase-1 (HaTre-1) is closely related to other soluble trehalases with conserved signature features and functional sites. Read More

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Modelling studies determing the mode of action of anthelmintics inhibiting in vitro trehalose-6-phosphate phosphatase (TPP) of Anisakis simplex s.l.

Exp Parasitol 2018 Jan 21;184:46-56. Epub 2017 Nov 21.

Department of Botany and Nature Protection, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Poland.

The trehalose-6-phosphate phosphatase (TPP) enzyme is involved in the synthesis of trehalose, the main sugar in the energy metabolism of nematodes. TPP is a member of the HAD-like hydrolase superfamily and shows a robust and specific phosphatase activity for the substrate trehalose-6-phosphate. The presence of conserved active sites of TPP in closely related nematodes and its absence in humans makes it a promising target for antiparasitic drugs. Read More

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January 2018

Molecular Packing, Hydrogen Bonding, and Fast Dynamics in Lysozyme/Trehalose/Glycerol and Trehalose/Glycerol Glasses at Low Hydration.

J Phys Chem B 2017 10 3;121(40):9437-9451. Epub 2017 Oct 3.

Univ. Lille, CNRS, UMR 8207, UMET, Unité Matériaux Et Transformations, F-59000 Lille, France.

Water and glycerol are well-known to facilitate the structural relaxation of amorphous protein matrices. However, several studies evidenced that they may also limit fast (∼picosecond-nanosecond, ps-ns) and small-amplitude (∼Å) motions of proteins, which govern their stability in freeze-dried sugar mixtures. To determine how they interact with proteins and sugars in glassy matrices and, thereby, modulate their fast dynamics, we performed molecular dynamics (MD) simulations of lysozyme/trehalose/glycerol (LTG) and trehalose/glycerol (TG) mixtures at low glycerol and water concentrations. Read More

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October 2017

Influence of a Small Amount of Glycerol on the Trehalose Bioprotective Action Analyzed In Situ During Freeze-Drying of Lyzozyme Formulations by Micro-Raman Spectroscopy.

J Pharm Sci 2017 10 15;106(10):2988-2997. Epub 2017 Jun 15.

Unité Matériaux Et Transformations, UMR CNRS 8207, Université de Lille 1, Villeneuve d'Ascq Cédex 59655, France. Electronic address:

Micro-Raman spectroscopy gives the original opportunity to monitor simultaneously the operating process and the protein structure from in situ investigations along the 3 stages of the freeze-drying (FD) process. This opportunity was used for determining how a small amount of glycerol enhances the bioprotective efficiency of trehalose during FD of lysozyme formulations. Three lysozyme formulations were analyzed: lysozyme dissolved in DO (wt% 1:9), in trehalose-DO mixture (wt% 1:1:8), and in the trehalose-glycerol-DO mixture (wt% 1:1:0. Read More

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October 2017

An unexplored remarkable PNIPAM-osmolyte interaction study: An integrated experimental and simulation approach.

J Colloid Interface Sci 2017 Oct 30;504:417-428. Epub 2017 May 30.

Discipline of Pharmaceutical Sciences, School of Health Sciences, University of KwaZulu - Natal (UKZN), Westville Campus, Durban 4000, South Africa.

We investigate the aggregation and collapse of water soluble amphiphilic polymer, poly(N-isopropylacrylamide) (PNIPAM), in aqueous solution containing variable amount of trehalose, sucrose and sorbitol. The effect of these osmolytes on the coil to globular transition of the PNIPAM is studied by the use of comprehensive biophysical techniques like UV-visible spectroscopy, fluorescence spectroscopy, dynamic light scattering and Fourier transform infrared spectroscopy (FTIR). The polarization induced by these additives promotes the collapsed state of PNIPAM at much lower temperature as compared to the pure PNIPAM in aqueous solution. Read More

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October 2017

Revisiting Hydrogen Bond Thermodynamics in Molecular Simulations.

J Chem Theory Comput 2017 Jun 1;13(6):2851-2857. Epub 2017 Jun 1.

Institute of Chemistry and The Fritz Haber Research Center, The Hebrew University , Jerusalem 91904, Israel.

In processes involving aqueous solutions and in almost every biomolecular interaction, hydrogen bonds play important roles. Though weak compared to the covalent bond, hydrogen bonds modify the stability and conformation of numerous small and large molecules and modulate their intermolecular interactions. We propose a simple methodology for extracting hydrogen bond strength from atomistic level simulations. Read More

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Molecular dynamics approach to understand the denaturing effect of a millimolar concentration of dodine on a λ-repressor and counteraction by trehalose.

Phys Chem Chem Phys 2017 May;19(20):13160-13171

Department of Chemistry, Indian Institute of Technology, Guwahati, India.

Commonly used denaturants for protein denaturation are conventionally required in high concentrations in order to produce their effects. In this study, a molecular dynamics simulation of a mutated version of the N-terminal domain of a λ-repressor is carried out in the presence of a 10 millimolar (mM) concentration of dodine. Such a small concentration is found to be effective for denaturation of the protein. Read More

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Cryoprotective roles of trehalose and alginate oligosaccharides during frozen storage of peeled shrimp (Litopenaeus vannamei).

Food Chem 2017 Aug 28;228:257-264. Epub 2017 Jan 28.

Zhejiang Provincial Key Laboratory of Health Risk Factors for Seafood, College of Food Science and Pharmacy, Zhejiang Ocean University, China.

Cryoprotective saccharides are widely accepted additives that reduce thawing loss, maintain texture, and retard protein denaturation in the frozen seafood. The present study aimed to investigate the roles of trehalose and alginate oligosaccharides on cryoprotection of frozen shrimp, primarily focusing on the interactions between myosin and saccharide molecules using a molecular dynamics (MD) simulation analysis. The results indicated that soaking in the trehalose and alginate oligosaccharides solutions markedly reduced thawing and cooking losses in frozen shrimp, with respective values decreasing to 6. Read More

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