9,331 results match your criteria bombyx mori


Bombyx mori nucleopolyhedrovirus ptp and egt genes are dispensable for triggering enhanced locomotory activity and climbing behavior in Bombyx mandarina larvae.

J Invertebr Pathol 2021 May 7:107604. Epub 2021 May 7.

Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address:

Baculoviruses are classic pathogens that alter host behavior to enhance their dispersal and transmission. While viral protein tyrosine phosphatase (ptp) has been considered as a critical factor for inducing enhanced locomotory activity, preceding investigations have reported that viral ecdysteroid UDP-glucosyltransferase (egt) contributes to triggering climbing behavior in some virus and host species. Here we found that both egt and ptp were dispensable for these abnormal behaviors in Bombyx mandarina larvae induced by Bombyx mori nucleopolyhedrovirus, thus implying that there is an unknown core mechanism of baculovirus-induced alteration of host behaviors. Read More

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Composite Film with Antibacterial Gold Nanoparticles and Silk Fibroin for Treating Multidrug-Resistant -Infected Wounds.

ACS Biomater Sci Eng 2021 May 1;7(5):1827-1835. Epub 2020 Oct 1.

Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China.

The progressively increasing degree of multiple antibiotic resistance in Gram-negative bacteria challenges the treatment of severe bacterial infection-induced chronic skin wounds. To address this problem, we developed a straightforward strategy to prepare a composite film consisting of antibacterial nanoparticles (4,6-diamino-2-pyrimidinethiol-functionalized gold nanoparticles, DAPT-Au NPs) and a silk fibroin (SF) mixed-matrix membrane (DAPT-Au-SF MMM) as a wound dressing for treating multidrug-resistant (MDR) induced infection. The good hydrophilicity of SF allows the highly effective release of DAPT-Au NPs from the composite film to combat pathogens within minutes. Read More

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Silver nanoparticles are effective in controlling microsporidia.

Mater Sci Eng C Mater Biol Appl 2021 Jun 17;125:112106. Epub 2021 Apr 17.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing 400716, China. Electronic address:

Many approaches and technologies have been developed as treatments for microsporidian, infections but effective, broad-spectrum, and sustainable therapeutic approaches have not been found. Silver nanoparticles (AgNPs) have antimicrobial activity and are widely used against many different pathogens. AgNPs provide an opportunity to develop formulations that will control microsporidia. Read More

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Toxic effects of acute exposure to polystyrene microplastics and nanoplastics on the model insect, silkworm Bombyx mori.

Environ Pollut 2021 Apr 30;285:117255. Epub 2021 Apr 30.

Max Planck Partner Group, Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou, China; Key Laboratory for Molecular Animal Nutrition, Ministry of Education, Zhejiang University, Hangzhou, China. Electronic address:

Microplastics and nanoplastics (MPs and NPs, respectively) are major contaminants of environmental concern due to their potentially detrimental effects on aquatic and terrestrial ecosystems. However, little is known about their potential toxicity in terrestrial organisms. Here, we used the model insect silkworm (Bombyx mori) to evaluate the potential hazardous effects of acute exposure (72 h) to polystyrene (PS) MPs and NPs at physiological, molecular, and biochemical levels as well as their impact on pathogen infection. Read More

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Toll9 from functions as a pattern recognition receptor that shares features with Toll-like receptor 4 from mammals.

Proc Natl Acad Sci U S A 2021 May;118(19)

Guangdong Laboratory for Lingnan Modern Agriculture, Laboratory of Insect Molecular Biology and Biotechnology, Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, 510642 Guangzhou, China;

Toll/Toll-like receptors (TLRs) are key regulators of the innate immune system in both invertebrates and vertebrates. However, while mammalian TLRs directly recognize pathogen-associated molecular patterns, the insect Toll pathway is thought to be primarily activated by binding Spätzle cytokines that are processed from inactive precursors in response to microbial infection. Phylogenetic and structural data generated in this study supported earlier results showing that Toll9 members differ from other insect Tolls by clustering with the mammalian TLR4 group, which recognizes lipopolysaccharide (LPS) through interaction with myeloid differentiation-2 (MD-2)-like proteins. Read More

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Septin homologs cooperating in the Proliferative Stage of Microsporidia Nosema bombycis.

J Invertebr Pathol 2021 May 4;183:107600. Epub 2021 May 4.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; Chongqing Key Laboratory of Microsporidia Infection and Prevention, Chongqing 400716, China; Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agricultural, Southwest University, Chongqing 400716, China; College of Life Science, Chongqing Normal University, Chongqing 400047, China. Electronic address:

The single-celled pathogen Nosema bombycis, that can infect silkworm Bombyx mori and other lepidoptera including Spodoptera, is the first identified Microsporidia which has diplokaryotic nuclei throughout the life cycle. Septin proteins can form highly ordered filaments, bundles or ring structures related to the cytokinesis in fungi. Here, three septin proteins (NbSeptin1, NbSeptin2 and NbSeptin3) from Nosema bombycis CQ I are described. Read More

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Bmcas-1 plays an important role in response against BmNPV infection in vitro.

Arch Insect Biochem Physiol 2021 May 5:e21793. Epub 2021 May 5.

Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China.

Apoptosis, as one kind of innate immune system, is involved in host response against pathogens innovation. Caspases play a vital role in the execution stage of host cell apoptosis. It has been reported that Bmcaspase-1 (Bmcas-1) has a close relationship with Bombyx mori nucleopolyhedrovirus (BmNPV) infection for its differentially expressed patterns after viral infection. Read More

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MnO-capped silk fibroin (SF) nanoparticles with chlorin e6 (Ce6) encapsulation for augmented photo-driven therapy by modulating the tumor microenvironment.

J Mater Chem B 2021 May;9(17):3677-3688

State Key Laboratory of Silkworm Genome Biology, School of Materials and Energy, Southwest University, Chongqing 400715, China.

Silk fibroin (SF), derived from Bombyx mori, is a category of fibrous protein with outstanding potential for applications in the biomedical and biotechnological fields. In spite of its many advantageous properties, the exploration of SF as a versatile nanodrug precursor for tumor therapy has still been restricted in recent years. Herein, a multifunctional SF-derived nanoplatform was facilely developed via encapsulating the photosensitizer chlorin e6 (Ce6) into MnO2-capped SF nanoparticles (NPs). Read More

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Transcriptome reveal the response to Cry1Ac toxin in susceptible Bombyx mori.

Arch Insect Biochem Physiol 2021 May 5:e21794. Epub 2021 May 5.

Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China.

Bombyx mori as a representative in Lepidoptera is an important economic insect in agriculture production. Bacillus thuringiensis (Bt) is a bacterial pathogen in silkworm production. Understanding how silkworm respond to Bt-toxin can provide guidance to cultivate resistant silkworm strains. Read More

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Development of insect cell line using CRISPR technology.

Prog Mol Biol Transl Sci 2021 26;180:1-20. Epub 2021 Feb 26.

Synthetic Nanobiotechnology and Biomachines Group, ERA Synthetic Biology, Centre for Synthetic Biology and Precision Medicine, CSIR, Pretoria, South Africa. Electronic address:

In this chapter, we delineated the methods of CRISPR technology that has been used for the development of engineered insect cell line. We elaborated on how CRISPR/Cas9 genome editing in Drosophila melanogaster, Bombyx mori, Spodoptera frugiperda (Sf9 and Sf21), and Mosquitoes enabled the use of model or non-model insect system in various biological and medical applications. Also, the application of synthetic baculovirus genome along with CRISPR/Cas9 vector system to enable genome editing of insect cell systems for treatment of communicable and non-communicable diseases. Read More

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February 2021

Carbon Nanotubes/Regenerated Silk Composite as a Three-Dimensional Printable Bio-Adhesive Ink with Self-Powering Properties.

ACS Appl Mater Interfaces 2021 May 3;13(18):21007-21017. Epub 2021 May 3.

Dipartimento di Ingegneria Civile e Ambientale, Università degli Studi di Perugia, Strada di Pentima 4, Terni 05100, Italy.

In this study, regenerated silk (RS) obtained from cocoons is compounded with carboxyl-functionalized carbon nanotubes (f-CNTs) in an aqueous environment for the fabrication of functional bio-adhesives. Molecular interactions between RS and carboxyl groups of CNTs result in structural increase of the β-sheet formation, obtaining a resistant adhesive suitable for a wet biological substrate. Moreover, the functionalization of CNTs promotes their dispersion in RS, thus enabling the production of films with controlled electrical conductivity. Read More

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Loss of p24 from the Bombyx mori nucleopolyhedrovirus genome results in the formation of cuboidal occlusion bodies.

Virology 2021 Apr 1;559:173-181. Epub 2021 Apr 1.

Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan. Electronic address:

Some insect viruses produce the occlusion body (OB), a large crystalline particle comprising a viral protein that occludes virions to protect them from harsh environments. The shapes and sizes of OBs are diverse depending on baculovirus species, but the detailed molecular mechanism determining them has yet to be totally clarified yet. Here we generated Bombyx mori nucleopolyhedrovirus (BmNPV) mutants of the p24 gene that encodes a viral capsid protein and found that p24-mutated BmNPVs produced cuboidal OBs with a slightly larger size than typical truncated octahedral OBs produced by wild-type BmNPVs. Read More

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iTRAQ-based quantitative proteomic analysis of silkworm infected with Beauveria bassiana.

Mol Immunol 2021 Apr 28;135:204-216. Epub 2021 Apr 28.

School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212018, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, 212018, China. Electronic address:

Beauveria bassiana is a harmful pathogen to the economically important insect silkworm, always causes serious disease to the silkworm, which results in great losses to the sericulture industry. In order to explore the silkworm (Bombyx mori) response to B. bassiana infection, differential proteomes of the silkworm responsive to B. Read More

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Changes in expressions of ecdysteroidogenic enzyme and ecdysteroid signaling genes in relation to Bombyx embryonic development.

J Exp Zool A Ecol Integr Physiol 2021 Apr 30. Epub 2021 Apr 30.

Department of Biology, National Museum of Natural Science, Taichung, Taiwan, ROC.

Although the role of ecdysteroids in regulating egg diapause process in Bombyx mori is well documented, temporal changes in expression levels of genes involved in ecdysteroid biosynthesis and its downstream signaling are less well understood. In the present study, we studied changes in expression levels of genes involved in ecdysteroid biosynthesis and its downstream signaling during embryonic development of B. mori. Read More

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Synthesis, Quantification, and Characterization of Fatty Acid Amides from In Vitro and In Vivo Sources.

Molecules 2021 Apr 27;26(9). Epub 2021 Apr 27.

Department of Chemistry, University of South Florida, 4202 E. Fowler Ave., Tampa, FL 33620, USA.

Fatty acid amides are a diverse family of underappreciated, biologically occurring lipids. Herein, the methods for the chemical synthesis and subsequent characterization of specific members of the fatty acid amide family are described. The synthetically prepared fatty acid amides and those obtained commercially are used as standards for the characterization and quantification of the fatty acid amides produced by biological systems, a fatty acid amidome. Read More

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Effect of on the Liver Protection of Non-Alcoholic Fatty Liver Disease Based on In Vitro and In Vivo Models.

Curr Issues Mol Biol 2021 Apr 28;43(1). Epub 2021 Apr 28.

Department of Food and Nutrition, College of BioNano Technology, Gachon University, Gyeonggi-do 13120, Korea.

Edible insects, (silkworm; SW), which feed on mulberry leaves, have been consumed by humans for a long time as supplements or traditional medication. Non-alcoholic fatty liver disease (NAFLD) is a liver metabolic disorder that affects many people worldwide. We examined the hepatoprotective effects of SW using in vitro and high-fat and high-fructose (HFHF) diet-induced obese in vivo model mice by real-time PCR, immunoblot analysis, and fecal microbiota analysis. Read More

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Evaluating Oxygen Tensions Related to Bone Marrow and Matrix for MSC Differentiation in 2D and 3D Biomimetic Lamellar Scaffolds.

Int J Mol Sci 2021 Apr 13;22(8). Epub 2021 Apr 13.

Department of Biotechnology, Middle East Technical University, 06800 Ankara, Turkey.

The physiological O microenvironment of mesenchymal stem cells (MSCs) and osteoblasts and the dimensionality of a substrate are known to be important in regulating cell phenotype and function. By providing the physiologically normoxic environments of bone marrow (5%) and matrix (12%), we assessed their potential to maintain stemness, induce osteogenic differentiation, and enhance the material properties in the micropatterned collagen/silk fibroin scaffolds that were produced in 2D or 3D. Expression of osterix (OSX) and vascular endothelial growth factor A (VEGFA) was significantly enhanced in the 3D scaffold in all oxygen environments. Read More

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Pupae Efficiently Produce Recombinant AAV2/HBoV1 Vectors with a Nuclear Polyhedrosis Virus Expression System.

Viruses 2021 04 18;13(4). Epub 2021 Apr 18.

School of Life Sciences, Jiangsu University, Zhenjiang 212013, China.

Recombinant adeno-associated virus (AAV) vectors have broad application prospects in the field of gene therapy. The establishment of low-cost and large-scale manufacturing is now the general agenda for industry. The baculovirus-insect cell/larva expression system has great potential for these applications due to its scalability and predictable biosafety. Read More

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Estrogen-Related Receptor Influences the Hemolymph Glucose Content by Regulating Midgut Trehalase Gene Expression in the Last Instar Larvae of .

Int J Mol Sci 2021 Apr 21;22(9). Epub 2021 Apr 21.

Biological Science Research Center, Southwest University, Chongqing 400716, China.

The expression of trehalase in the midgut of insects plays an important role in glucose supply to the hemolymph. Energy metabolism is usually regulated by the estrogen-related receptor (ERR). A decrease in ATP levels is caused by the ERR hindering glycolysis. Read More

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Changes in Amino Acid Profiles and Bioactive Compounds of Thai Silk Cocoons as Affected by Water Extraction.

Molecules 2021 Apr 2;26(7). Epub 2021 Apr 2.

Research Unit of Process and Product Development of Functional Foods, Department of Food Technology and Nutrition, Faculty of Technology, Mahasarakham University, Kantarawichai, Mahasarakham 44150, Thailand.

Silk proteins have many advantageous components including proteins and pigments. The proteins-sericin and fibroin-have been widely studied for medical applications due to their good physiochemical properties and biological activities. Various strains of cocoon display different compositions such as amino-acid profiles and levels of antioxidant activity. Read More

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Lepidopteran wing scales contain abundant cross-linked film-forming histidine-rich cuticular proteins.

Commun Biol 2021 Apr 22;4(1):491. Epub 2021 Apr 22.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.

Scales are symbolic characteristic of Lepidoptera; however, nothing is known about the contribution of cuticular proteins (CPs) to the complex patterning of lepidopteran scales. This is because scales are resistant to solubilization, thus hindering molecular studies. Here we succeeded in dissolving developing wing scales from Bombyx mori, allowing analysis of their protein composition. Read More

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Homeodomain proteins POU-M2, antennapedia and abdominal-B are involved in regulation of the segment-specific expression of the clip-domain serine protease gene CLIP13 in the silkworm, Bombyx mori.

Insect Sci 2021 Apr 16. Epub 2021 Apr 16.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China.

Clip-domain serine proteases (CLIPs) play important roles in insect innate immunity and development. Our previous studies indicated that CLIP13, an epidermis-specific gene, was involved in cuticle remodeling during molting and metamorphosis in the silkworm, Bombyx mori. However, the transcriptional regulatory mechanism and regulatory pathways of CLIP13 remained unclear. Read More

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Encapsulation, release and insecticidal activity of (L.) seed oil.

Heliyon 2021 Mar 24;7(3):e06557. Epub 2021 Mar 24.

Department of Agricultural Chemicals, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, 741 252, Nadia, West Bengal, India.

(L.) seed oil is effective for its insecticidal and larvicidal activities. However, its low aqueous solubility, high photosensitivity, and high volatility restrict its application for the control of agricultural pests. Read More

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Comparisons in temperature and photoperiodic-dependent diapause induction between domestic and wild mulberry silkworms.

Sci Rep 2021 Apr 13;11(1):8052. Epub 2021 Apr 13.

Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, 386-8567, Japan.

The bivoltine strain of the domestic silkworm, Bombyx mori, has two generations per year. It shows a facultative diapause phenotype determined by environmental conditions, including photoperiod and temperature, and nutrient conditions during embryonic and larval development of the mother. However, it remains unclear how the environmental signals received during development are selectively utilized as cues to determine alternative diapause phenotypes. Read More

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Mutation of P-element somatic inhibitor induces male sterility in the diamondback moth, Plutella xylostella.

Pest Manag Sci 2021 Apr 11. Epub 2021 Apr 11.

CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences/Institute of Plant Physiology and Ecology, Shanghai, China.

Background: Genetic manipulation of sex determination pathways in insects provides the basis for a broad range of strategies to benefit agricultural security and human health. The P-element somatic inhibitor (PSI) protein, an exon splicing silencer that promotes male-specific splicing of dsx, plays a critical role in male sexual differentiation and development. The functions of PSI have been characterized in the lepidopteran model species Bombyx mori. Read More

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Low concentration acetamiprid-induced oxidative stress hinders the growth and development of silkworm posterior silk glands.

Pestic Biochem Physiol 2021 May 11;174:104824. Epub 2021 Mar 11.

School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, PR China; Sericulture Institute of Soochow University, Suzhou, Jiangsu 215123, PR China. Electronic address:

Acetamiprid is a new type of nicotinic insecticide that is widely used in pest control. Its environmental residues may cause silkworm cocooning disorder. In this study, silkworms that received continuous feeding of low concentration acetamiprid (0. Read More

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Transcriptome analysis reveals changes in silkworm energy metabolism during Nosema bombycis infection.

Pestic Biochem Physiol 2021 May 27;174:104809. Epub 2021 Feb 27.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Ministry of Agriculture and Rural Affairs, Chongqing 400716, China. Electronic address:

Energy metabolism is important for the proliferation of microsporidia in infected host cells, but there is limited information on the host response. The energy metabolism response of silkworm (Bombyx mori) to microsporidia may help manage Nosema bombycis infections. We analyzed differentially expressed genes in the B. Read More

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Towards a flexible electrochemical biosensor fabricated from biocompatible Bombyx mori silk.

Biosens Bioelectron 2021 Jul 28;183:113204. Epub 2021 Mar 28.

Institute of Nano- and Biotechnologies (INB), FH Aachen, Campus Jülich, Heinrich-Mußmann-Strasse 1, 52428, Jülich, Germany; Forschungszentrum Jülich GmbH, Institute of Biological Information Processing (IBI-3), Wilhelm-Johnen-Strasse 6, 52425, Jülich, Germany. Electronic address:

In modern days, there is an increasing relevance of and demand for flexible and biocompatible sensors for in-vivo and epidermal applications. One promising strategy is the implementation of biological (natural) polymers, which offer new opportunities for flexible biosensor devices due to their high biocompatibility and adjustable biodegradability. As a proof-of-concept experiment, a biosensor was fabricated by combining thin- (for Pt working- and counter electrode) and thick-film (for Ag/AgCl quasi-reference electrode) technologies: The biosensor consists of a fully bio-based and biodegradable fibroin substrate derived from silk fibroin of the silkworm Bombyx mori combined with immobilized enzyme glucose oxidase. Read More

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Studies on Microbial Quality, Protein Yield, and Antioxidant Properties of Some Frozen Edible Insects.

Int J Food Sci 2021 23;2021:5580976. Epub 2021 Mar 23.

Food Science and Technology, Science Division, International College, Mahidol University, Buddhamonthon 4 Rd, Nakhonpathom, Salaya 73170, Thailand.

This research investigated the microbial quality and the protein contents of Thai commercial frozen products of silkworm (), bamboo caterpillar (), and field cricket (). Total mesophilic aerobic counts were about 8-8.4 log cfu/g, while lactic acid bacteria numbers were up to 5. Read More

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Sob gene is critical to wing development in Bombyx mori and Tribolium castaneum.

Insect Sci 2021 Apr 6. Epub 2021 Apr 6.

State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, College of Biotechnology, Southwest University, Chongqing, China.

The development of insect appendages requires the expression of multiple genes in a strict spatial and temporal order. The odd-skipped family genes are vital transcriptional factors involved in embryonic development. The development and morphogenesis of the insect wing requires multiple transcription factors to regulate the expression of wing patterning genes at the transcriptional level. Read More

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