18,733 results match your criteria body regeneration


Forever young: stem cell and plant regeneration one century after Haberlandt 1921.

Protoplasma 2021 Jul 22. Epub 2021 Jul 22.

Department of Biology, Stanford University, Stanford, CA, 94305, USA.

Plants are characterized by a post-embryonic mode of organ development, which results in a need for these photoautotrophic organisms to regenerate lost parts in the course of their life cycle. This capacity depends on the presence of "pluripotent stem cells," which are part of the meristems within the plant body. One hundred years ago, the botanist Gottlieb Haberlandt (1854-1945) published experiments showing wounding-induced callus formation, which led ultimately to plant regeneration in tissue culture and thence to the techniques of "plant biotechnology," with practical applications for mankind. Read More

View Article and Full-Text PDF

Genome-Wide DNA (hydroxy)methylation reveals the individual epigenetic landscape importance on osteogenic phenotype acquisition in periodontal ligament cells.

J Periodontol 2021 Jul 22. Epub 2021 Jul 22.

Department of Dentistry, University of Taubaté, Taubaté, Brazil.

Background: Mesenchymal cells' biology has been an important investigative tool to maximize bone regeneration through tissue engineering. Here we used mesenchymal cells from periodontal ligament (PDLCs) with high (h-) and low (l-) osteogenic potential, isolated from different donors, to investigate the impact of the individual epigenetic and transcriptional profiles on the osteogenic potential.

Materials And Methods: Genome-wide and gene-specific DNA (hydroxy)methylation, mRNA expression and immunofluorescence analysis were carried out in h- and l-PDLCs at DMEM (non-induced to osteogenesis) and OM (induced - 3 and 10 days of osteogenic differentiation) groups in vitro. Read More

View Article and Full-Text PDF

Recent Advances in the Application of Two-Dimensional Nanomaterials for Neural Tissue Engineering and Regeneration.

ACS Biomater Sci Eng 2021 Jul 22. Epub 2021 Jul 22.

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P.R. China.

The complexity of the nervous system structure and function, and its slow regeneration rate, makes it more difficult to treat compared to other tissues in the human body when an injury occurs. Moreover, the current therapeutic approaches including the use of autografts, allografts, and pharmacological agents have several drawbacks and can not fully restore nervous system injuries. Recently, nanotechnology and tissue engineering approaches have attracted many researchers to guide tissue regeneration in an effective manner. Read More

View Article and Full-Text PDF

Neural mechanisms underlying the Rubber Hand Illusion: A systematic review of related neurophysiological studies.

Brain Behav 2021 Jul 21:e02124. Epub 2021 Jul 21.

Department of Neurology, Christian Doppler Klinik, Paracelsus Medical University, Salzburg, Austria.

Introduction: Many researchers took advantage of the well-established rubber hand illusion (RHI) paradigm to explore the link between the sense of body ownership and the different brain structures and networks. Here, we aimed to review the studies that have investigated this phenomenon by means of neurophysiological techniques.

Methods: The MEDLINE, accessed by Pubmed and EMBASE electronic databases, was searched using the medical subject headings: "Rubber hand illusion" AND "Transcranial magnetic stimulation (TMS)" OR "Evoked potentials (EP)" OR "Event related potentials (ERP)" OR "Electroencephalography (EEG)". Read More

View Article and Full-Text PDF

Mapping Human Pluripotent Stem Cell-derived Erythroid Differentiation by Single-cell Transcriptome Analysis.

Genomics Proteomics Bioinformatics 2021 Jul 17. Epub 2021 Jul 17.

CAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences/China National Center of Bioinformation, Beijing 100101, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; Sino-Danish College, University of Chinese Academy of Sciences, Beijing 100190, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China; Beijing Key Laboratory of Genome and Precision Medicine Technologies, Beijing, 100101, China. Electronic address:

There is an imbalance between the supply and demand of functional red blood cells (RBCs) in clinical applications. This imbalance can be addressed by regenerating RBCs using several in vitro methods. Induced pluripotent stem cells (iPSCs) can handle the low supply of cord blood and the ethical issues in embryonic stem cell research and provide a promising strategy to eliminate immune rejection. Read More

View Article and Full-Text PDF

Kupffer cell restoration after partial hepatectomy is mainly driven by local cell proliferation in IL-6-dependent autocrine and paracrine manners.

Cell Mol Immunol 2021 Jul 19. Epub 2021 Jul 19.

Laboratory of Liver Diseases, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD, USA.

Kupffer cells (KCs), which are liver-resident macrophages, originate from the fetal yolk sac and represent one of the largest macrophage populations in the body. However, the current data on the origin of the cells that restore macrophages during liver injury and regeneration remain controversial. Here, we address the question of whether liver macrophage restoration results from circulating monocyte infiltration or local KC proliferation in regenerating livers after partial hepatectomy (PHx) and uncover the underlying mechanisms. Read More

View Article and Full-Text PDF

Drosophila attack inhibits hyphal regeneration and defense mechanisms activation for the fungus Trichoderma atroviride.

ISME J 2021 Jul 19. Epub 2021 Jul 19.

Advanced Genomics Unit-Langebio, Guanajuato, Mexico.

The capability to respond to wounding is a process shared by organisms of different kingdoms that can result in the regeneration of whole-body parts or lost structures or organs. Filamentous fungi constitute a rich food source that ensures survival and reproduction of their predators and are therefore continuously exposed to mechanical damage. Nevertheless, our understanding of how fungi respond to wounding and predators is scarce. Read More

View Article and Full-Text PDF

Injectable catalyst-free "click" organic-inorganic nanohybrid (click-ON) cement for minimally invasive in vivo bone repair.

Biomaterials 2021 Jul 6;276:121014. Epub 2021 Jul 6.

Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55905, USA; Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, 55905, USA. Electronic address:

Injectable polymers have attracted intensive attention in tissue engineering and drug delivery applications. Current injectable polymer systems often require free-radical or heavy-metal initiators and catalysts for the crosslinking process, which may be extremely toxic to the human body. Here, we report a novel polyhedral oligomeric silsesquioxane (POSS) based strain-promoted alkyne-azide cycloaddition (SPAAC) "click" organic-inorganic nanohybrids (click-ON) system that can be click-crosslinked without any toxic initiators or catalysts. Read More

View Article and Full-Text PDF

Roles of Extracellular Vesicles in Cerebral Protection of Ischemic Stroke.

Neuro Endocrinol Lett 2021 Jul 19;42(3):160-170. Epub 2021 Jul 19.

Department of Cardiothoracic Surgery, the First Affiliated Hospital of Ganna Medical University, 23 Qinnian Road, Zhanggong, Ganzhou, Jiangxi 341000, China.

Stroke is the second-leading cause of death worldwide and exhibits a high disability rate. Ischemic stroke accounts for approximately 80% of all stroke cases. Inflammatory responses induced by innate immunity are involved in all stages of stroke-related injury, including early cerebral-infarction tissue repair and regeneration after ischemia. Read More

View Article and Full-Text PDF

Molecular Mechanisms of Intermittent Fasting-induced Ischemic Tolerance.

Cond Med 2020 Feb;3(1):9-17

Department of Physiology, National University of Singapore, Singapore.

Diet is a significant factor in determining human well-being. Excessive eating and/or diets with higher than needed amounts of carbohydrates, salt, and fat are known to cause metabolic disorders and functional changes in the body. To compensate the ill effects, many designer diets including the Mediterranean diet, the Okinawa diet, vegetarian/vegan diets, keto diet, anti-inflammatory diet, and the anti-oxidant diet have been introduced in the past 2 decades. Read More

View Article and Full-Text PDF
February 2020

Spinal Palpation Error and Its Impact on Skin Marker-Based Spinal Alignment Measurement in Adult Spinal Deformity.

Front Bioeng Biotechnol 2021 23;9:687323. Epub 2021 Jun 23.

Department of Development and Regeneration, Faculty of Medicine, Institute for Orthopaedic Research and Training, KU Leuven, Leuven, Belgium.

Spinal alignment measurement in spinal deformity research has recently shifted from using mainly two-dimensional static radiography toward skin marker-based motion capture approaches, allowing three-dimensional (3D) assessments during dynamic conditions. The validity and accuracy of such skin marker-based methods is highly depending on correct marker placement. In this study we quantified, for the first time, the 3D spinal palpation error in adult spinal deformity (ASD) and compared it to the error in healthy spines. Read More

View Article and Full-Text PDF

Alginate and alginate composites for biomedical applications.

Asian J Pharm Sci 2021 May 5;16(3):280-306. Epub 2020 Nov 5.

Department of Biotechnology Engineering, International Islamic University Malaysia, Kuala Lumpur 50728, Malaysia.

Alginate is an edible heteropolysaccharide that abundantly available in the brown seaweed and the capsule of bacteria such as sp. and sp. Owing to alginate gel forming capability, it is widely used in food, textile and paper industries; and to a lesser extent in biomedical applications as biomaterial to promote wound healing and tissue regeneration. Read More

View Article and Full-Text PDF

Local Translation in Nervous System Pathologies.

Front Integr Neurosci 2021 29;15:689208. Epub 2021 Jun 29.

Laboratory of Local Translation in Neurons and Glia, Achucarro Basque Center for Neuroscience, Leioa, Spain.

Dendrites and axons can extend dozens to hundreds of centimeters away from the cell body so that a single neuron can sense and respond to thousands of stimuli. Thus, for an accurate function of dendrites and axons the neuronal proteome needs to be asymmetrically distributed within neurons. Protein asymmetry can be achieved by the transport of the protein itself or the transport of the mRNA that is then translated at target sites in neuronal processes. Read More

View Article and Full-Text PDF

The relationship between three-dimensional craniofacial and upper airway anatomical variables and severity of obstructive sleep apnoea in adults.

Eur J Orthod 2021 Jul 16. Epub 2021 Jul 16.

Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, China.

Objective: The aim of this study was to evaluate the relationship between three-dimensional craniofacial and upper airway anatomical variables and severity of obstructive sleep apnoea (OSA) using cone beam computed tomography (CBCT).

Methods: This was a prospective observational study, consisted of 95 adult OSA whose polysomnography and CBCT were available. Three-dimensional craniofacial and upper airway anatomical analysis were performed using 12 CBCT variables, including sagittal and vertical jaw relationships, maxillary width, the volume, length and minimum axial area of upper airway. Read More

View Article and Full-Text PDF

Do the positioning variables of the cage contribute to adjacent facet joint degeneration? Radiological and clinical analysis following intervertebral fusion.

Ann Transl Med 2021 May;9(9):776

Department of Spine Surgery, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.

Background: Compared to other risk factors, adjacent facet joint degeneration (AFD) is the main contributor to adjacent segment disease (ASD). The interbody cage may be a potential indirect risk of AFD. This study investigated the correlations among the lumbar sagittal balance parameters, the inter-body cage's intraoperative positioning variables, and adjacent facet joint degeneration following the transforaminal lumbar interbody fusion (TLIF) technique. Read More

View Article and Full-Text PDF

A self-powered implantable and bioresorbable electrostimulation device for biofeedback bone fracture healing.

Proc Natl Acad Sci U S A 2021 Jul;118(28)

Department of Materials Science and Engineering, University of Wisconsin-Madison, Madison, WI 53706;

Electrostimulation has been recognized as a promising nonpharmacological treatment in orthopedics to promote bone fracture healing. However, clinical applications have been largely limited by the complexity of equipment operation and stimulation implementation. Here, we present a self-powered implantable and bioresorbable bone fracture electrostimulation device, which consists of a triboelectric nanogenerator for electricity generation and a pair of dressing electrodes for applying electrostimulations directly toward the fracture. Read More

View Article and Full-Text PDF

Long-term clinical outcomes of periodontal regeneration with Enamel Matrix Derivative (EMD): a retrospective cohort study with a mean follow-up of 10 years.

J Periodontol 2021 Jul 14. Epub 2021 Jul 14.

Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland.

Background: Despite the large body of evidence on the efficacy of Enamel Matrix Derivative (EMD) in the treatment of periodontal intrabony defects, few studies reported long-term data (≥ 10-year).

Methods: Periodontal patients treated with regenerative surgery with EMD between 1999 and 2012 were invited to participate in a clinical examination. The following clinical parameters were recorded and compared at baseline (T0), 6 months after surgery (T1) and after at least 8 years of follow-up (T2): probing depth (PD), gingival recession (GR), clinical attachment level (CAL), plaque and bleeding scores. Read More

View Article and Full-Text PDF

The Enigmatic Reissner's Fiber and the Origin of Chordates.

Front Neuroanat 2021 23;15:703835. Epub 2021 Jun 23.

Centro de Investigación Biomédica, Facultad de Medicina, Universidad Diego Portales, Santiago, Chile.

Reissner's fiber (RF) is a secreted filament that floats in the neural canal of chordates. Since its discovery in 1860, there has been no agreement on its primary function, and its strong conservation across chordate species has remained a mystery for comparative neuroanatomists. Several findings, including the chemical composition and the phylogenetic history of RF, clinical observations associating RF with the development of the neural canal, and more recent studies suggesting that RF is needed to develop a straight vertebral column, may shed light on the functions of this structure across chordates. Read More

View Article and Full-Text PDF

A novel therapeutic phosphate-based glass improves full-thickness wound healing in a rat model.

Biotechnol J 2021 Jul 9:e2100031. Epub 2021 Jul 9.

Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania.

Wound healing is a highly dynamic process and innovative therapeutic approaches are currently developed to address challenges of providing optimal wound care. In this study, phosphate-based glasses in the (CuO) ·(KPO ) ·(ZnO) ·(Ag O) system (CuKPO ZnAg), with different CuO/ KPO ratios were prepared by melt-quenching technique. Constant Cu concentrations were released from the samples during immersion in Simulated Body Fluid (SBF), while Zn concentrations were slightly decreased over time. Read More

View Article and Full-Text PDF

Importance of brain alterations in spinal cord injury.

Sci Prog 2021 Jul-Sep;104(3):368504211031117

Beijing Key Laboratory for Biomaterials and Neural Regeneration, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, China.

Spinal cord injury (SCI) destroys the sensorimotor pathway and blocks the information flow between the peripheral nerve and the brain, resulting in autonomic function loss. Numerous studies have explored the effects of obstructed information flow on brain structure and function and proved the extensive plasticity of the brain after SCI. Great progress has also been achieved in therapeutic strategies for SCI to restore the "re-innervation" of the cerebral cortex to the limbs to some extent. Read More

View Article and Full-Text PDF

Emerging Potential of Exosomes on Adipogenic Differentiation of Mesenchymal Stem Cells.

Front Cell Dev Biol 2021 22;9:649552. Epub 2021 Jun 22.

Department of Tissue Engineering, School of Fundamental Science, China Medical University, Shenyang, China.

The mesenchymal stem cells have multidirectional differentiation potential and can differentiate into adipocytes, osteoblasts, cartilage tissue, muscle cells and so on. The adipogenic differentiation of mesenchymal stem cells is of great significance for the construction of tissue-engineered fat and the treatment of soft tissue defects. Exosomes are nanoscale vesicles secreted by cells and widely exist in body fluids. Read More

View Article and Full-Text PDF

2D biointerfaces to study stem cell-ligand interactions.

Acta Biomater 2021 Jul 5. Epub 2021 Jul 5.

Department of Instructive Biomaterials Engineering, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, P.O. Box 616, 6200 MD Maastricht, the Netherlands. Electronic address:

Stem cells have great potential in the field of tissue engineering and regenerative medicine due to their inherent regenerative capabilities. However, an ongoing challenge within their clinical translation is to elicit or predict the desired stem cell behavior once transplanted. Stem cell behavior and function are regulated by their interaction with biophysical and biochemical signals present in their natural environment (i. Read More

View Article and Full-Text PDF

Axonal injury alters the extracellular glial environment of the axon initial segment and allows substantial mitochondrial influx into axon initial segment.

J Comp Neurol 2021 Jul 7. Epub 2021 Jul 7.

Functional Anatomy and Neuroscience, Nagoya University Graduate School of Medicine, Nagoya, Japan.

The axon initial segment (AIS) is structurally and functionally distinct from other regions of the axon, yet alterations in the milieu of the AIS after brain injury have not been well characterized. In this study, we have examined extracellular and intracellular changes in the AIS after hypoglossal nerve injury. Microglial adhesions to the AIS were rarely observed in healthy controls, whereas microglial adhesions to the AIS became apparent in the axonal injury model. Read More

View Article and Full-Text PDF

Autoimmunity as an Etiological Factor of Cancer: The Transformative Potential of Chronic Type 2 Inflammation.

Front Cell Dev Biol 2021 21;9:664305. Epub 2021 Jun 21.

Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, FL, United States.

Recent epidemiological studies have found an alarming trend of increased cancer incidence in adults younger than 50 years of age and projected a substantial rise in cancer incidence over the next 10 years in this age group. This trend was exemplified in the incidence of non-cardia gastric cancer and its disproportionate impact on non-Hispanic white females under the age of 50. The trend is concurrent with the increasing incidence of autoimmune diseases in industrialized countries, suggesting a causal link between the two. Read More

View Article and Full-Text PDF

Graphene Oxide: A Promising Material for Regenerative Medicine and Tissue Engineering.

Biomol Concepts 2020 Dec 31;11(1):182-200. Epub 2020 Dec 31.

Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Regenerative medicine and tissue engineering have been considered pioneer fields in the life sciences, with an ultimate goal of restoring or switching lost or impaired body parts. Graphene oxide (GO) is the product of graphene oxidation and presents a great opportunity to make substantial progress in the field of regenerative medicine; for example, it supports the possibility of creating a cellular niche for stem cells on a nanoparticle surface. GO creates a fascinating structure for regulating stem cell behavior, as it can potentially applied to the noninvasive chase of stem cells , the liberation of active biological factors from stem cell-containing delivery systems, and the intracellular delivery of factors such as growth factors, DNA, or synthetic proteins in order to modulate stem cell differentiation and proliferation. Read More

View Article and Full-Text PDF
December 2020

Effects of aerobic exercise on gut microbiota in adolescents with subthreshold mood syndromes and healthy adolescents: A 12-week, randomized controlled trial.

J Affect Disord 2021 Jun 20;293:363-372. Epub 2021 Jun 20.

School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing. Electronic address:

Background: Animal and human studies have revealed reciprocal association between exercise and gut-brain axis. However, the clinical evidence from randomized controlled trials (RCT) are still limited to directly assess the effects of aerobic exercise on gut microbiota. To fill this gap, we conducted this 12-week RCT in both groups of adolescents with and without sub-threshold mood symptoms. Read More

View Article and Full-Text PDF

Cellular, Metabolic, and Developmental Dimensions of Whole-Body Regeneration in .

Cold Spring Harb Perspect Biol 2021 Jul 6. Epub 2021 Jul 6.

Department of Genetics and Evolution, Institute of Genetics and Genomics in Geneva (iGE3), Faculty of Sciences, University of Geneva, Geneva 4, Switzerland.

Here we discuss the developmental and homeostatic conditions necessary for regeneration. is characterized by populations of adult stem cells paused in the G2 phase of the cell cycle, ready to respond to injury signals. The body column can be compared to a blastema-like structure, populated with multifunctional epithelial stem cells that show low sensitivity to proapoptotic signals, and high inducibility of autophagy that promotes resistance to stress and starvation. Read More

View Article and Full-Text PDF

Aging-related upregulation of the homeobox gene caudal represses intestinal stem cell differentiation in Drosophila.

PLoS Genet 2021 Jul 6;17(7):e1009649. Epub 2021 Jul 6.

Laboratory for Aging and Stem Cell Research, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

The differentiation efficiency of adult stem cells undergoes a significant decline in aged animals, which is closely related to the decline in organ function and age-associated diseases. However, the underlying mechanisms that ultimately lead to this observed decline of the differentiation efficiency of stem cells remain largely unclear. This study investigated Drosophila midguts and identified an obvious upregulation of caudal (cad), which encodes a homeobox transcription factor. Read More

View Article and Full-Text PDF

Valproic acid stimulates myogenesis in pluripotent stem cell-derived mesodermal progenitors in a NOTCH-dependent manner.

Cell Death Dis 2021 Jul 5;12(7):677. Epub 2021 Jul 5.

Laboratory of Translational Cardiomyology, Department of Development and Regeneration, Stem Cell Research Institute, KU Leuven, 3000, Leuven, Belgium.

Muscular dystrophies are debilitating neuromuscular disorders for which no cure exists. As this disorder affects both cardiac and skeletal muscle, patients would benefit from a cellular therapy that can simultaneously regenerate both tissues. The current protocol to derive bipotent mesodermal progenitors which can differentiate into cardiac and skeletal muscle relies on the spontaneous formation of embryoid bodies, thereby hampering further clinical translation. Read More

View Article and Full-Text PDF

Comprehensive in vitro comparison of cellular and osteogenic response to alternative biomaterials for spinal implants.

Mater Sci Eng C Mater Biol Appl 2021 Aug 10;127:112251. Epub 2021 Jun 10.

ETH Zurich, Institute for Biomechanics, Zurich, Switzerland. Electronic address:

A variety of novel biomaterials are emerging as alternatives to conventional metals and alloys, for use in spinal implants. These promise potential advantages with respect to e.g. Read More

View Article and Full-Text PDF