364,712 results match your criteria blood samples

Nasopharyngeal cancer cell-derived exosomal PD-L1 inhibits CD8+ T cell activity and promotes immune escape.

Cancer Sci 2022 May 22. Epub 2022 May 22.

Department of Radiation Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou, P. R. China.

Programmed cell death ligand 1 (PD-L1) is an immune surface protein that binds to programmed cell death 1 (PD-1) and allows tumors to evade T cell immunity. This study aims to define the role of PD-L1 shuttled by tumor cell-derived exosomes in the immune escape of nasopharyngeal carcinoma (NPC). PD-L1 expression was determined in the exosomes isolated from the plasma of NPC patients or from NPC cells. Read More

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Stability and reproducibility of proteomic profiles in epidemiological studies: Comparing the Olink and SOMAscan platforms.

Proteomics 2022 May 21:e2100170. Epub 2022 May 21.

Channing Division of Network Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, MA, USA.

Limited data exist on the performance of high-throughput proteomics profiling in epidemiological settings, including the impact of specimen collection and within-person variability over time. Thus, the Olink (972 proteins) and SOMAscan7Kv4.1 (7,322 proteoforms of 6,596 proteins) assays were utilized to measure protein concentrations in archived plasma samples from the Nurses' Health Studies and Health Professionals Follow-Up Study. Read More

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Association of bone-related biomarkers with femoral neck bone strength.

BMC Musculoskelet Disord 2022 May 21;23(1):482. Epub 2022 May 21.

Department of Orthopedics, The General Hospital of Western Theater Command, Rongdu Avenue No. 270, Chengdu, 610083, China.

Background: Femoral neck fractures are the worst consequence of osteoporosis (OP), and its early prevention and treatment have become a public health problem. This study aims to investigate the relationship of bone-related biomarkers, femoral neck bone mineral density (BMD) and maximum load (L), selecting the indicator which can reflect femoral neck bone loss and reduced bone strength.

Methods: A total of 108 patients were recruited from January 2017 to December 2019. Read More

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Blood compatibility of widely used central venous catheters; an experimental study.

Sci Rep 2022 May 21;12(1):8600. Epub 2022 May 21.

Department of Clinical Sciences, Karolinska Institute, Danderyd Hospital, Sweden and Bactiguard AB, Tullinge, Stockholm, Sweden.

An inserted central venous catheter (CVC) is considered foreign material by the inert host defence systems and induce inflammation and thrombus formation. The objective of this study was to evaluate blood compatibility of six commonly used CVCs. Three coated and three uncoated CVC materials were tested in a modified Chandler loop model. Read More

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Urinary cadmium and peripheral blood telomere length predict the risk of renal function impairment: a study of 547 community residents of Shanxi, China.

Environ Sci Pollut Res Int 2022 May 21. Epub 2022 May 21.

Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan, 030001, China.

Few reports have investigated the predictive value of urinary cadmium (UCd) and telomere length on renal function impairment. Therefore, we constructed nomogram models, using a cross-sectional survey to analyze the potential function of UCd and telomere length in renal function impairment risk. We randomly selected two community populations in Shanxi, China, and general information of the subjects was collected through face-to-face questionnaire surveys. Read More

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Costus speciosus extract protects against the oxidative damage of zearalenone via modulation of inflammatory cytokines, Nrf2 and iNOS gene expression in rats.

Toxicon 2022 May 18. Epub 2022 May 18.

Food Toxicology & Contaminants Department, National Research Center, Dokki, Cairo, Egypt. Electronic address:

Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin that induces severe health disturbances in humans and animals. This study aimed to determine the bioactive compounds in Costus speciosus extract (CSE) using GC-MS and evaluate its protective capability against ZEN-induced oxidative damage, genotoxicity, and cytotoxicity in rats. Six groups of male Sprague Dawley rats were treated orally for 15 days including the control group, CSE-treated groups at low (200 mg/kg b. Read More

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Myxovirus resistance protein A for discriminating between viral and bacterial lower respiratory tract infections in children - The TREND study.

Clin Microbiol Infect 2022 May 18. Epub 2022 May 18.

Pediatric Emergency Unit, Sachs' Children and Youth Hospital, Stockholm, Sweden; Department of Global Public Health, Karolinska Institutet, Stockholm, Sweden.

Objective: Discriminating between viral and bacterial lower respiratory tract infection (LRTI) in children is challenging, leading to an excessive use of antibiotics. Myxovirus resistance protein A (MxA) is a promising biomarker for viral infections. The primary aim of the study was to assess differences in blood MxA levels between children with viral and bacterial LRTI. Read More

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SARS-COV-2 RBD (Receptor binding domain) mutations and variants (A sectional-analytical study).

Microb Pathog 2022 May 18:105595. Epub 2022 May 18.

Department of Virology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.

An essential step in SARS-CoV-2 infection is binding the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein to the ACE2 receptor on the surface of host cells. Therefore, variation in this region can have crucial effects on clinical outcomes and the emergence of variants of concern (VOCs) and variants of interest (VOIs). In this cross-sectional descriptive study, 54 patients with SARS-COV-2 infection were enrolled. Read More

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Higher free thyroxine associated with PFAS exposure in first trimester. The Odense Child Cohort.

Environ Res 2022 May 18:113492. Epub 2022 May 18.

Department of Endocrinology, Odense University Hospital, Søndre Blvd. 29, 5000, Odense C, Denmark; Department of Clinical Research, Faculty of Health Sciences, University of Southern Denmark, 5000, Odense, Denmark.

Background: Perfluoroalkyl substances (PFAS) are endocrine disrupting chemicals with elimination half-lives ranging from four to eight years. Experimental studies found PFAS able to interfere with thyroid hormone-binding proteins. During the first 20 weeks of gestation (GW), the fetus is reliant on placental transfer of maternal thyroid hormones, mainly free thyroxine (FT4). Read More

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Nickel and cadmium tissue bioaccumulation and blood parameters in Chelon auratus and Mugil cephalus from Anzali free zone in the south Caspian Sea (Iran) and Faro Lake (Italy): A comparative analysis.

J Trace Elem Med Biol 2022 May 16;72:126999. Epub 2022 May 16.

Department of Veterinary Science, Polo Universitario dell'Annunziata, University of Messina, 98168 Messina, Italy. Electronic address:

Background: Analysis of heavy metal concentrations in fish blood is a valuable tool in environmental pollution monitoring. Among different type of fish, detritivorous fish are a very good indicator for monitoring pollution and environmental stress, along with hematological studies, which are an important indicator in eco-toxicological and biological studies.

Aim: The aim of this study was to evaluate the influence of environment on bioaccumulation of Ni and Cd and on blood parameters in Chelon auratus and Mugil cephalus (a detritivorous fish which is widely distributed in the world) captured in Caspian Sea and Faro Lake. Read More

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Effect of flunixin meglumine on pain-related behaviours following cautery disbudding in dairy goat kids.

Vet Res Commun 2022 May 21. Epub 2022 May 21.

Estación Experimental Agropecuaria Salta, INTA, Cerrillos, 4403, Salta, Argentina.

The aim of this research was to evaluate the physiological and behavioural parameters in Saanen goat kids treated with flunixin meglumine compared with no analgesic treatment after cautery disbudding using a device fueled by propane gas. At 7 days of age, 30 goat kids were randomly allocated to three groups: Sham (Sh) control, (i.e. Read More

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Correlation of chemokines and growth factors with radiation-induced liver injury after interstitial high dose rate (HDR) brachytherapy of liver metastases.

J Cancer Res Clin Oncol 2022 May 21. Epub 2022 May 21.

Department of Radiology and Nuclear Medicine, Otto-von-Guericke-University, Magdeburg, Germany.

Background: Locoregional therapies, as imaging-guided tumor-directed procedures, are emerging treatment strategies in the management of primary and secondary liver malignancies such as e.g. colorectal cancer liver metastases. Read More

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Club cell protein (CC)16 as potential lung injury marker in a porcine 72 h polytrauma model.

Eur J Trauma Emerg Surg 2022 May 21. Epub 2022 May 21.

Experimental Radiology, Department of Radiology and Nuclear Medicine, Otto-von-Guericke University, 39120, Magdeburg, Germany.

Background: Polytrauma and respiratory tract damage after thoracic trauma cause about 25% of mortality among severely injured patients. Thoracic trauma can lead to the development of severe lung complications such as acute respiratory distress syndrome, and is, therefore, of great interest for monitoring in intensive care units (ICU). In recent years, club cell protein (CC)16 with its antioxidant properties has proven to be a potential outcome-related marker. Read More

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Acquired platelet defects are responsible for non-surgical bleeding in left ventricular assist device recipients.

Artif Organs 2022 May 21. Epub 2022 May 21.

Fischell Department of Bioengineering, A. James Clark School of Engineering, University of Maryland, College Park, MD, USA.

Background: Left ventricular assist devices (LVADs) have been used as a standard treatment option for patients with advanced heart failure. However, these devices are prone to adverse events. Non-surgical bleeding (NSB) is the most common complication in patients with continuous flow (CF) LVADs. Read More

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The mediating effect of DNA methylation in the association between maternal sleep during pregnancy and offspring adiposity status: a prospective cohort study.

Clin Epigenetics 2022 May 20;14(1):66. Epub 2022 May 20.

Department of Developmental and Behavioral Pediatrics, Pediatric Translational Medicine Institute, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, 1678 Dong Fang Road, Shanghai, 200127, China.

Background: Childhood overweight/obesity is a global public health concern. It is important to identify its early-life risk factors. Maternal poor sleep is common in late pregnancy, and previous studies indicated that poor sleep may influence the offspring's adiposity status. Read More

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WBC image classification and generative models based on convolutional neural network.

BMC Med Imaging 2022 May 20;22(1):94. Epub 2022 May 20.

Department of Cyber Security, Ewha Womans University, 52, Ewhayeodae-gil, Seodaemun-gu, Seoul, 03760, Republic of Korea.

Background: Computer-aided methods for analyzing white blood cells (WBC) are popular due to the complexity of the manual alternatives. Recent works have shown highly accurate segmentation and detection of white blood cells from microscopic blood images. However, the classification of the observed cells is still a challenge, in part due to the distribution of the five types that affect the condition of the immune system. Read More

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Stroke-derived neutrophils demonstrate higher formation potential and impaired resolution of CD66b + driven neutrophil extracellular traps.

BMC Neurol 2022 May 20;22(1):186. Epub 2022 May 20.

Department of Neurosurgery, KLW St Paulus Corporation, St Marien Academic Hospital Lünen, Westfälische Wilhelms-University Münster, Altstadtstrasse 23, 44534, Lünen, Germany.

Background: Recent evidence suggests a merging role of immunothrombosis in the formation of arterial thrombosis. Our study aims to investigate its relevance in stroke patients.

Methods: We compared the peripheral immunological profile of stroke patients vs. Read More

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Alterations of the gut microbial community structure and function with aging in the spontaneously hypertensive stroke prone rat.

Sci Rep 2022 May 20;12(1):8534. Epub 2022 May 20.

Department of Anesthesiology, Baylor College of Medicine, Houston, TX, 77030, USA.

Gut dysbiosis, a pathological imbalance of bacteria, has been shown to contribute to the development of hypertension (HT), systemic- and neuro-inflammation, and blood-brain barrier (BBB) disruption in spontaneously hypertensive stroke prone rats (SHRSP). However, to date individual species that contribute to HT in the SHRSP model have not been identified. One potential reason, is that nearly all studies of the SHRSP gut microbiota have analyzed samples from rats with established HT. Read More

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Quantification of immunosuppressants from one 3.2 mm dried blood spot by a novel cold-induced phase separation based LC-MS/MS method.

Anal Chim Acta 2022 Jun 1;1210:339889. Epub 2022 May 1.

Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China. Electronic address:

Dried blood spots (DBS) have been regarded as a promising alternative for therapeutic drug monitoring (TDM) of immunosuppressants (ISDs) for over fifteen years. Nonetheless, there are still three main issues impeding its preference: (i) the requirement of relatively large disc; (ii) the controversial and intricate desorption approaches; (iii) the lack of feasible extraction strategies. For improvement, this work described a new LC-MS/MS method realizing quantification of four ISDs from one piece of 3. Read More

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Benzidine color-crystal test; modified test to confirm the presence of blood stain.

Hemraj Verma

Forensic Sci Int 2022 Jun 21;335:111313. Epub 2022 Apr 21.

State Forensic Science Laboratory, Jaipur 302016, Rajasthan, India. Electronic address:

In this study, author presented a color-crystal test, to confirm the presence of blood stain. In this test, blue colored micro-crystals were observed in case of positive blood sample. Since author included same reagents used for primary benzidine color test, hence in positive samples, used reagent impart blue color to the crystals and aid in proper visibility to the developed micro-crystals, observed under 20x and 40x magnification. Read More

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High lead exposure and clinical signs of toxicosis in wild Nile crocodiles (Crocodylus niloticus) from a World Heritage site: Lake St Lucia estuarine system, South Africa.

Chemosphere 2022 May 17:134977. Epub 2022 May 17.

Department of Nature Conservation, Tshwane University of Technology, South Africa.

Lead (Pb) exposure is a widespread wildlife conservation threat, but impacts on reptile populations remain poorly documented. In this study, we examined Pb exposure and accumulation in a wild population of Nile crocodiles (Crocodylus niloticus) at Lake St Lucia, South Africa. Recreational angling has occurred in the area since the 1930s and incidental ingestion of Pb fishing weights has previously been identified as a major source of Pb poisoning in the local crocodile population. Read More

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Usefulness of real-time RT-PCR to understand the kinetics of SARS-CoV-2 in blood: A prospective study.

J Clin Virol 2022 Apr 26;152:105166. Epub 2022 Apr 26.

Department of Clinical Microbiology, Hospital Universitario de la Princesa, Madrid, Spain.

Background: SARS-CoV-2 viral load and kinetics assessed in serial blood samples from hospitalised COVID-19 patients by RT-PCR are poorly understood.

Methods: We conducted an observational, prospective case series study in hospitalised COVID-19 patients. Clinical outcome data (Intensive Care Unit admission and mortality) were collected from all patients until discharge. Read More

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Age-associated B cells indicate disease activity in rheumatoid arthritis.

Cell Immunol 2022 May 6;377:104533. Epub 2022 May 6.

Department of Rheumatology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. Electronic address:

Age-associated B cells (ABCs) are characterized by CD11c and T-bet expression. ABCs are elevated in several autoimmune diseases and may be associated with RA. This study aimed to investigate ABCs' role in RA and identify potential factors affecting ABCs in RA patients. Read More

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Assessment of cell-free DNA (cfDNA) concentrations in the perioperative period can predict risk of recurrence in patients with non-metastatic breast cancer.

Surg Oncol 2022 Apr 3;42:101753. Epub 2022 Apr 3.

Department of Breast Surgery, Cork University Hospital, Cork, Ireland; SURGUVANT Research Lab, University College Cork, Cork, Ireland.

Background: Circulating cell-free DNA (cfDNA) is a potential non-invasive biomarker of disease status in patients with cancer, and provides important diagnostic and prognostic information in breast cancer. The goal of this study was to quantify cfDNA concentrations during the perioperative period and investigate its potential utility to detect recurrence outcomes in patients with breast cancer.

Methods: Sixty-two (n = 62) patients with non-metastatic breast cancer, undergoing curative-intent surgery were screened for inclusion. Read More

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Serological evidence of the circulation of the Rift Valley fever virus in sheep and goats slaughtered in Yaoundé, Cameroon.

Vet Med Sci 2022 May 20. Epub 2022 May 20.

Department of Virology, Centre Pasteur of Cameroon, Yaoundé, Cameroon.

Background: Rift Valley fever (RVF) is an emerging mosquito-borne haemorrhagic fever disease capable of causing severe outbreaks with high mortality and morbidity in human, livestock, and wildlife species, particularly in Africa. The onset of the disease in humans is often preceded by epizootic circulation in animals. Therefore, this study was conducted to investigate the seroprevalence of Rift Valley fever virus (RVFV) infection in animals slaughtered in the "Marché huitième" slaughterhouse in Yaoundé, Cameroon. Read More

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Preinduction Serum Vitamin D3 Levels and Induction Chemotherapy Remission Rates in Patients with Acute Leukemia.

Nutr Cancer 2022 May 20:1-6. Epub 2022 May 20.

Department of Clinical Hematology, King George's Medical University, Chowk, Lucknow, India.

Vitamin D deficiency(<20 ng/mL) is a common condition, associated with an inferior prognosis in some cancers. However, the prognostic significance of vitamin D deficiency in acute leukemia is largely unknown. The present study aimed to assess the baseline status of vitamin D [25-(OH) D3] and find its association with induction remission rate and mortality using standard chemotherapy in patients with acute leukemia. Read More

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Circulating tumor DNA-based MRD assessment in patients with CLL treated with obinutuzumab, acalabrutinib and venetoclax.

Clin Cancer Res 2022 May 20. Epub 2022 May 20.

University Hospital Cologne, Cologne, Germany.

Background: With the advent of highly efficacious time-limited combination treatments in CLL, minimal residual disease (MRD) assessment has gained importance as a measure for therapeutic success. The currently most widely used method is multicolor flow cytometry, which detects circulating CLL cells in the peripheral blood. However, it seems to be less sensitive for the detection of MRD in the lymph node compartment. Read More

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Accuracy of the Standard GlucoNavii Mentor in Blood Glucose Monitoring According to International Organization for Standardization 15197:2013 Criteria.

JMIR Diabetes 2022 May 20;7(2):e20774. Epub 2022 May 20.

CORESEARCH Srl, Center for Outcomes Research and Clinical Epidemiology, Pescara, Italy.

This study was performed to assess the system accuracy of the blood glucose monitoring system SD GlucoNavii Mentor (SD Biosensor Inc, Korea). The study procedures were based on International Organization for Standardization 15197:2013, in that capillary blood samples from 100 participants' fingertips were measured with three reagent system lots of the self-monitoring blood glucose system. Samples were collected for comparison measurements on a hexokinase-based glucose analyzer (Cobas Integra400 Plus, Roche Instrument Center, Switzerland). Read More

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Blood cell quantification on dry blood samples: toward patient-centric complete blood counts.

Bioanalysis 2022 May 20. Epub 2022 May 20.

KTH Royal Institute of Technology, Micro & Nanosystems, Stockholm, 10044, Sweden.

Performing complete blood counts from patients' homes could have a transformative impact on e-based healthcare. Blood microsampling and sample drying are enabling elements for patient-centric healthcare. The aim of this study was to investigate the potential of dry blood samples for image-based cell quantification of red and white blood cells. Read More

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Evaluation of IL-17A expressions in high-grade glial tumors receiving radiotherapy.

Niger J Clin Pract 2022 May;25(5):582-588

Department of Microbiology, Çukurova University School of Medicine, Adana, Turkey.

Aim: In this study, we aimed to investigate the interleukin-17A (IL-17A) levels in patients with high-grade glial tumors before receiving radiotherapy, immediately after radiotherapy, and 3 months after radiotherapy.

Patients And Methods: A total of 33 patients who applied to Adana City Training and Research Hospital, Department of Radiation Oncology between December 2016 and May 2018 was included in this study. A total of three blood samples was taken from each patient to assess IL-17A levels before and after radiotherapy and 3 months after the completion of radiotherapy. Read More

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