1,495 results match your criteria biosensor targeting

Biochemical Activity Architectures Visualized-Using Genetically Encoded Fluorescent Biosensors to Map the Spatial Boundaries of Signaling Compartments.

Acc Chem Res 2021 May 5. Epub 2021 May 5.

Departments of Pharmacology, Bioengineering, and Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States.

ConspectusAll biological processes arise through the coordinated actions of biochemical pathways. How such functional diversity is achieved by a finite cast of molecular players remains a central mystery in biology. Spatial compartmentation-the idea that biochemical activities are organized around discrete spatial domains within cells-was first proposed nearly 40 years ago and has become firmly rooted in our understanding of how biochemical pathways are regulated to ensure specificity. Read More

View Article and Full-Text PDF

Rapid Detection of Using Multiple Cross Displacement Amplification Combined With Nanoparticles-Based Lateral Flow.

Front Cell Infect Microbiol 2021 13;11:622402. Epub 2021 Apr 13.

Department of Respiratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China.

is an opportunistic, ubiquitous, saprophytic mold which can cause infection in the lungs, nose, eyes, brain, and bones in humans, especially in immunocompromised patients. However, it is difficult to diagnose infection quickly. Here, we introduce a new detection method, namely multiple cross displacement amplification (MCDA) combined with nanoparticle-based lateral flow biosensor (LFB) (MCDA-LFB), which was proved to be fast, reliable, and simple for detecting . Read More

View Article and Full-Text PDF

Rapid and Easy-to-Use Method for Accurate Characterization of Target Binding and Kinetics of Magnetic Particle Bioconjugates for Biosensing.

Sensors (Basel) 2021 Apr 15;21(8). Epub 2021 Apr 15.

Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov Str., 119991 Moscow, Russia.

The ever-increasing use of magnetic particle bioconjugates (MPB) in biosensors calls for methods of comprehensive characterization of their interaction with targets. Label-free optical sensors commonly used for studying inter-molecular interactions have limited potential for MPB because of their large size and multi-component non-transparent structure. We present an easy-to-use method that requires only three 20-min express measurements to determine the key parameters for selection of optimal MPB for a biosensor: kinetic and equilibrium characteristics, and a fraction of biomolecules on the MPB surface that are capable of active targeting. Read More

View Article and Full-Text PDF

Plasmonic Biosensors for Single-Molecule Biomedical Analysis.

Biosensors (Basel) 2021 Apr 15;11(4). Epub 2021 Apr 15.

Nanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, BIST, and CIBER-BBN, Campus UAB, 08193 Barcelona, Spain.

The rapid spread of epidemic diseases (i.e., coronavirus disease 2019 (COVID-19)) has contributed to focus global attention on the diagnosis of medical conditions by ultrasensitive detection methods. Read More

View Article and Full-Text PDF

A Real-Time Thermal Sensor System for Quantifying the Inhibitory Effect of Antimicrobial Peptides on Bacterial Adhesion and Biofilm Formation.

Sensors (Basel) 2021 Apr 14;21(8). Epub 2021 Apr 14.

Department of Microsystems Engineering (IMTEK)-Laboratory of Sensors, University of Freiburg, 79110 Freiburg, Germany.

The increasing rate of antimicrobial resistance (AMR) in pathogenic bacteria is a global threat to human and veterinary medicine. Beyond antibiotics, antimicrobial peptides (AMPs) might be an alternative to inhibit the growth of bacteria, including AMR pathogens, on different surfaces. Biofilm formation, which starts out as bacterial adhesion, poses additional challenges for antibiotics targeting bacterial cells. Read More

View Article and Full-Text PDF

Multienzyme-Targeted Fluorescent Probe as a Biosensing Platform for Broad Detection of Pesticide Residues.

Anal Chem 2021 May 28;93(18):7079-7085. Epub 2021 Apr 28.

Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensor Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China.

Pesticide residues, significantly hampering the overall environmental and human health, have become an increasingly severe issue. Thus, developing rapid, cost-effective, and sensitive tools for monitoring the pesticide residues in food and water is extremely important. Compared to the conventional and chromatographic techniques, enzyme inhibition-based biosensors conjugated with the fluorogenic probes provide effective alternative methods for detecting pesticide residues due to the inherent advantages including high selectivity and sensitivity, simple operation, and capability of providing in situ and real-time information. Read More

View Article and Full-Text PDF

AQP8 is a crucial HO transporter in insulin-producing RINm5F cells.

Redox Biol 2021 Apr 1;43:101962. Epub 2021 Apr 1.

Institute of Clinical Biochemistry, Hannover Medical School, 30623, Hannover, Germany. Electronic address:

Peroxiporins are distinct aquaporins (AQP) which, beside water, also facilitate the bidirectional transport of hydrogen peroxide (HO) across cellular membranes. HO serves as the major reactive oxygen species that mediates essential cell signaling events. In pancreatic β-cells, HO has been associated with the regulation of cell growth but in excess it leads to failure of insulin secretion, making it important for diabetes mellitus (DM) pathogenesis. Read More

View Article and Full-Text PDF

Web resources facilitate drug discovery in treatment of COVID-19.

Drug Discov Today 2021 Apr 20. Epub 2021 Apr 20.

Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China; International Joint Research Center for Intelligent Biosensor Technology and Health, Central China Normal University, Wuhan 430079, China; Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, China.

The infectious disease Coronavirus 2019 (COVID-19) continues to cause a global pandemic and, thus, the need for effective therapeutics remains urgent. Global research targeting COVID-19 treatments has produced numerous therapy-related data and established data repositories. However, these data are disseminated throughout the literature and web resources, which could lead to a reduction in the levels of their use. Read More

View Article and Full-Text PDF

Sensitive quantitative detection of SARS-CoV-2 in clinical samples using digital warm-start CRISPR assay.

Biosens Bioelectron 2021 Jul 17;184:113218. Epub 2021 Apr 17.

Department of Biomedical Engineering, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT, 06030, United States. Electronic address:

Quantifying severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in clinical samples is crucial for early diagnosis and timely medical treatment of coronavirus disease 2019. Here, we describe a digital warm-start CRISPR (dWS-CRISPR) assay for sensitive quantitative detection of SARS-CoV-2 in clinical samples. The dWS-CRISPR assay is initiated at above 50 °C and overcomes undesired premature target amplification at room temperature, enabling accurate and reliable digital quantification of SARS-CoV-2. Read More

View Article and Full-Text PDF

Review on the recent progress in the development of fluorescent probes targeting enzymes.

Methods Appl Fluoresc 2021 Apr 30;9(3). Epub 2021 Apr 30.

Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensor Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, People's Republic of China.

Enzymes are very important for biological processes in a living being, performing similar or multiple tasks in and out of cells, tissues and other organisms at a particular location. The abnormal activity of particular enzyme usually caused serious diseases such as Alzheimer's disease, Parkinson's disease, cancers, diabetes, cardiovascular diseases, arthritis etc. Hence, nondestructive and real-time visualization for certain enzyme is very important for understanding the biological issues, as well as the drug administration and drug metabolism. Read More

View Article and Full-Text PDF

Array-Based Biosensors for Bacteria Detection: From the Perspective of Recognition.

Small 2021 Apr 18:e2006230. Epub 2021 Apr 18.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.

Array-based biosensors have shown as effective and powerful tools to distinguish intricate mixtures with infinitesimal differences among analytes such as nucleic acids, proteins, microorganisms, and other biomolecules. In array-based bacterial sensing, the recognition of bacteria is the initial step that can crucially influence the analytical performance of a biosensor array. Bacteria recognition as well as the signal readout and mathematical analysis are indispensable to ensure the discrimination ability of array-based biosensors. Read More

View Article and Full-Text PDF

Diagnostic and Therapeutic Nanomedicine.

Jinmyoung Joo

Adv Exp Med Biol 2021 ;1310:401-447

Department of Biomedical Engineering, Ulsan National Institute of Science and Technology, Ulsan, South Korea.

Nanotechnology has been widely applied to medical interventions for prevention, diagnostics, and therapeutics of diseases, and the application of nanotechnology for medical purposes, which is called as a term "nanomedicine" has received tremendous attention. In particular, the design and development of nanoparticle for biosensors have received a great deal of attention, since those are most impactful area of clinical translation showing potential breakthrough in early diagnosis of diseases such as cancers and infections. For example, the nanoparticles that have intrinsic unique features such as magnetic responsive characteristics or photoluminescence can be utilized for noninvasive visualization of inner body. Read More

View Article and Full-Text PDF

Rapid Quantum Dot Nanobead-mAb Probe-Based Immunochromatographic Assay for Antibody Monitoring of Infection.

Int J Nanomedicine 2021 29;16:2477-2486. Epub 2021 Mar 29.

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, People's Republic of China.

Purpose: Sensitive and selective point-of-care biosensor is an urgent pursuit of serological antibody detection to control parasite pathogen. For specific, quantitative and on-site screening of infection in livestock, a quantum dot nanobead-monoclonal antibody (QB-mAb) probe-based immunochromatographic assay (ICA) was developed by introducing a competitive sandwich strategy (QB-CICA).

Methods: In the QB-CICA, QB-mAb probes competed with serum antibody for a particular epitope, followed by immunocomplexes binding to capture antibody on the test line. Read More

View Article and Full-Text PDF

Discrimination of single-point mutations in unamplified genomic DNA via Cas9 immobilized on a graphene field-effect transistor.

Nat Biomed Eng 2021 Apr 5. Epub 2021 Apr 5.

Keck Graduate Institute, The Claremont Colleges, Claremont, CA, USA.

Simple and fast methods for the detection of target genes with single-nucleotide specificity could open up genetic research and diagnostics beyond laboratory settings. We recently reported a biosensor for the electronic detection of unamplified target genes using liquid-gated graphene field-effect transistors employing an RNA-guided catalytically deactivated CRISPR-associated protein 9 (Cas9) anchored to a graphene monolayer. Here, using unamplified genomic samples from patients and by measuring multiple types of electrical response, we show that the biosensors can discriminate within one hour between wild-type and homozygous mutant alleles differing by a single nucleotide. Read More

View Article and Full-Text PDF

Capture-free deactivation of CTCs in the bloodstream; a metastasis suppression method by electrostatic stimulation of the peripheral blood.

Biosens Bioelectron 2021 Jul 26;183:113194. Epub 2021 Mar 26.

Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran, P.O. Box 14395/515; Cancer Institute, Imam-Khomeini Hospital, Tehran University of Medical Sciences, P.O. BOX 13145-158, Tehran, Iran; UT&TUMS Cancer Electrotechnique Research Center, YAS Hospital, P.O. Box 1598718311, Tehran, Iran. Electronic address:

While limited investigations have been reported on CTC elimination and its profits, recently, some new works were reported on detection followed by the destruction of CTCs. Limitations and complications of CTC capturing procedures have highly reduced the chance of selective destruction of CTCs in the bloodstream in the therapeutic guidelines of the patients. Here, we selectively deactivated the invasive function of CTCs during their circulation in the bloodstream by exposing the whole blood to pure positive electrostatic charge stimulation (PPECS). Read More

View Article and Full-Text PDF

Engineering of Challenging G Protein-Coupled Receptors for Structure Determination and Biophysical Studies.

Molecules 2021 Mar 8;26(5). Epub 2021 Mar 8.

Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.

Membrane proteins such as G protein-coupled receptors (GPCRs) exert fundamental biological functions and are involved in a multitude of physiological responses, making these receptors ideal drug targets. Drug discovery programs targeting GPCRs have been greatly facilitated by the emergence of high-resolution structures and the resulting opportunities to identify new chemical entities through structure-based drug design. To enable the determination of high-resolution structures of GPCRs, most receptors have to be engineered to overcome intrinsic hurdles such as their poor stability and low expression levels. Read More

View Article and Full-Text PDF

Quantifying single-cell ERK dynamics in colorectal cancer organoids reveals EGFR as an amplifier of oncogenic MAPK pathway signalling.

Nat Cell Biol 2021 Apr 1;23(4):377-390. Epub 2021 Apr 1.

Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands.

Direct targeting of the downstream mitogen-activated protein kinase (MAPK) pathway to suppress extracellular-regulated kinase (ERK) activation in KRAS and BRAF mutant colorectal cancer (CRC) has proven clinically unsuccessful, but promising results have been obtained with combination therapies including epidermal growth factor receptor (EGFR) inhibition. To elucidate the interplay between EGF signalling and ERK activation in tumours, we used patient-derived organoids (PDOs) from KRAS and BRAF mutant CRCs. PDOs resemble in vivo tumours, model treatment response and are compatible with live-cell microscopy. Read More

View Article and Full-Text PDF

Expanding the Chemical Space of Succinate Dehydrogenase Inhibitors via the Carbon-Silicon Switch Strategy.

J Agric Food Chem 2021 Apr 28;69(13):3965-3971. Epub 2021 Mar 28.

Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensor Technology and Health of Ministry of Science and Technology, Central China Normal University, Luoyu Road 152, Wuhan 430079, People's Republic of China.

The carbon-silicon switch strategy has become a key technique for structural optimization of drugs to widen the chemical space, increase drug activity against targeted proteins, and generate novel and patentable lead compounds. Flubeneteram, targeting succinate dehydrogenase (SDH), is a promising fungicide candidate recently developed in China. We describe the synthesis of novel SDH inhibitors with enhanced fungicidal activity to enlarge the chemical space of flubeneteram by employing the C-Si switch strategy. Read More

View Article and Full-Text PDF

Continuous glucose monitoring systems - Current status and future perspectives of the flagship technologies in biosensor research.

Biosens Bioelectron 2021 Jun 2;181:113054. Epub 2021 Feb 2.

Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC, 27599, USA. Electronic address:

Diabetes mellitus is a chronic illness in the United States affecting nearly 120 million adults, as well as increasing in children under the age of 18. Diabetes was also the 7th leading cause of death in the United States with 270 K deaths in 2017. Diabetes is best managed by tight glycemic control, as achieving near-normal glucose levels is key to reduce the risk of microvascular complications. Read More

View Article and Full-Text PDF

High-throughput human DNA purification on a centrifugal microfluidic device for rapid forensic sex-typing.

Biosens Bioelectron 2021 Jun 13;181:113161. Epub 2021 Mar 13.

Department of Chemical Engineering (BK21 FOUR Integrated Engineering Program), Kyung Hee University, Yongin, 17104, South Korea. Electronic address:

We introduce a novel centrifugal disc for purifying nucleic acid (NA) in a high-throughput manner to perform the human sex-typing of forensic samples. The centrifugal disc was designed with double-sided etched channels to fabricate 30 extraction units, which was capable of purifying 30 forensic samples in a single run. In order to introduce the washing solution (70% ethanol) and the elution buffer for the 30 extraction units in an automatic manner, we designed the aliquoting chambers that were connected with a zigzag delivery channel. Read More

View Article and Full-Text PDF

Systematic Approach to DNA Aptamer Design Using Amino Acid-Nucleic Acid Hybrids (ANHs) Targeting Thrombin.

ACS Biomater Sci Eng 2021 04 23;7(4):1338-1343. Epub 2021 Mar 23.

Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.

Chemical modifications of innate DNA/RNA aptamers facilitate the improvement of their function. Herein, we report our modular strategy to manipulate a thrombin-binding DNA aptamer (TBA) to improve its anticoagulation activity and binding affinity. A set of amino acid conjugates, termed amino acid-nucleic acid hybrids or ANHs, was synthesized and incorporated into a TBA loop sequences. Read More

View Article and Full-Text PDF

Novel gene-encoded intermolecular FRET sensor for tracking glycosylation of CD147 in living cells.

Anal Bioanal Chem 2021 May 23;413(12):3193-3199. Epub 2021 Mar 23.

Hefei National Laboratory for Physical Sciences at Microscale, the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, Anhui, China.

CD147 is involved in various physiological processes and plays important roles for tumor metastasis. Glycosylation of the protein determines numerous functions of CD147. Up to now, hardly any sensor has been developed for detecting glycosylation of CD147 in live cells. Read More

View Article and Full-Text PDF

Kinetic Characterization of ASXL1/2-Mediated Allosteric Regulation of the BAP1 Deubiquitinase.

Mol Cancer Res 2021 Mar 17. Epub 2021 Mar 17.

The Wistar Institute, Philadelphia, Pennsylvania.

BAP1 is an ubiquitin hydrolase whose deubiquitinase activity is mediated by polycomb group-like protein ASXL2. Cancer-related mutations/deletions lead to loss-of-function by targeting the catalytic ubiquitin C-terminal hydrolase (UCH) or UCH37-like domain (ULD) domains of BAP1, and the latter disrupts binding to ASXL2, an obligate partner for BAP1 enzymatic activity. However, the biochemical and biophysical properties of domains involved in forming the enzymatically active complex are unknown. Read More

View Article and Full-Text PDF

SARS-CoV-2 S1 NanoBiT: A nanoluciferase complementation-based biosensor to rapidly probe SARS-CoV-2 receptor recognition.

Biosens Bioelectron 2021 May 2;180:113122. Epub 2021 Mar 2.

Ottawa Hospital Research Institute, Ottawa, ON, K1H 8L6, Canada; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, K1H 8M5, Canada. Electronic address:

As the COVID-19 pandemic continues, there is an imminent need for rapid diagnostic tools and effective antivirals targeting SARS-CoV-2. We have developed a novel bioluminescence-based biosensor to probe a key host-virus interaction during viral entry: the binding of SARS-CoV-2 viral spike (S) protein to its receptor, angiotensin-converting enzyme 2 (ACE2). Derived from Nanoluciferase binary technology (NanoBiT), the biosensor is composed of Nanoluciferase split into two complementary subunits, Large BiT and Small BiT, fused to the Spike S1 domain of the SARS-CoV-2 S protein and ACE2 ectodomain, respectively. Read More

View Article and Full-Text PDF

Biomaterials in treatment of Alzheimer's disease.

Neurochem Int 2021 05 5;145:105008. Epub 2021 Mar 5.

Department of Pharmaceutical Technology (Formulations), National Institute of Pharmaceutical Education and Research (NIPER-Guwahati), Sila Katamur (Halugurisuk), Changsari, Kamrup, 781101, Guwahati, Assam, India. Electronic address:

Alzheimer's disease (AD) is a non-recoverable progressive neurodegenerative disorder most prevalent but not limited to the old age population. After all the scientific efforts, there are still many unmet criteria and loopholes in available treatment and diagnostic strategies, limiting their efficacy. The poor drug efficacy is attributed to various biological hurdles, including blood-brain barrier (BBB) and peripheral side effects as most prominent ones and the lack of promising carriers to precisely deliver the drug to the brain by conserving its therapeutic potency. Read More

View Article and Full-Text PDF

Monotherapy and Combination Therapy Using Anti-Angiogenic Nanoagents to Fight Cancer.

Adv Mater 2021 Apr 8;33(15):e2005155. Epub 2021 Mar 8.

Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing, 211800, China.

Anti-angiogenic therapy, targeting vascular endothelial cells (ECs) to prevent tumor growth, has been attracting increasing attention in recent years, beginning with bevacizumab (Avastin) through its Phase II/III clinical trials on solid tumors. However, these trials showed only modest clinical efficiency; moreover, anti-angiogenic therapy may induce acquired resistance to the drugs employed. Combining advanced drug delivery techniques (e. Read More

View Article and Full-Text PDF

An ultrasensitive heart-failure BNP biosensor using B/N co-doped graphene oxide gel FET.

Biosens Bioelectron 2021 May 26;180:113114. Epub 2021 Feb 26.

Mechanical and Mechatronics Engineering, University of Waterloo, Waterloo, ON, Canada; Waterloo Institute for Nanotechnology (WIN), University of Waterloo, Waterloo, ON, Canada.

Heart failure (HF) is the number one cause of death in the world. B-type natriuretic peptide (BNP) is a recognized biomarker for HF and can be used for early detection. Field effect transistor (FET) biosensors have the ability to sense BNP in much shorter times than conventional clinical studies. Read More

View Article and Full-Text PDF

Analysis of Recent Bio-/Nanotechnologies for Coronavirus Diagnosis and Therapy.

Sensors (Basel) 2021 Feb 20;21(4). Epub 2021 Feb 20.

Laboratoire BAE, Université de Perpignan Via domitia, 66860 Perpignan, France.

Despite barrier measures and physical distancing tailored by the populations worldwide, coronavirus continues to spread causing severe health and social-economic problems. Therefore, researchers are focusing on developing efficient detection and therapeutic platforms for SARS-CoV2. In this context, various biotechnologies, based on novel molecules targeting the virus with high specificity and affinity, have been described. Read More

View Article and Full-Text PDF
February 2021

Lifetime-Engineered Ruby Nanoparticles (Tau-Rubies) for Multiplexed Imaging of μ-Opioid Receptors.

ACS Sens 2021 03 4;6(3):1375-1383. Epub 2021 Mar 4.

MQ Photonics, Faculty of Science and Engineering, Macquarie University, Sydney, 2109, Australia.

To address the growing demand for simultaneous imaging of multiple biomarkers in highly scattering media such as organotypic cell cultures, we introduce a new type of photoluminescent nanomaterial termed "tau-ruby" composed of ruby nanocrystals (AlO:Cr) with tunable emission lifetime. The lifetime tuning range from 2.4 to 3. Read More

View Article and Full-Text PDF

Rapid, Ultrasensitive, and Highly Specific Diagnosis of COVID-19 by CRISPR-Based Detection.

ACS Sens 2021 03 1;6(3):881-888. Epub 2021 Mar 1.

Experimental Research Center, Capital Institute of Pediatrics, Beijing100020, P. R. China.

Coronavirus Disease 2019 (COVID-19), which is caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2), has rapidly spread leading to a global pandemic. Here, we combined ultiple cross displacement amplification (MCDA) with RISPR-as12a-based etection to develop a novel diagnostic test (MCCD) and applied for the diagnosis of COVID-19, called COVID-19 MCCD. The MCCD protocol conducts reverse transcription MCDA (RT-MCDA) reaction for RNA templates followed by CRISPR-Cas12a/CrRNA complex detection of predefined target sequences after which degradation of a single-strand DNA (ssDNA) molecule confirms detection of the target sequence. Read More

View Article and Full-Text PDF