2,255 results match your criteria biomolecules fluorescent

Stimulated Chiral Light-Matter Interactions in Biological Microlasers.

ACS Nano 2021 May 14. Epub 2021 May 14.

School of Electrical and Electronic Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798 Singapore.

Chiral light-matter interactions have emerged as a promising area in biophysics and quantum optics. Great progress in enhancing chiral light-matter interactions have been investigated through passive resonators or spontaneous emission. Nevertheless, the interaction between chiral biomolecules and stimulated emission remains unexplored. Read More

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Potential Use of Exosomes as Diagnostic Biomarkers and in Targeted Drug Delivery: Progress in Clinical and Preclinical Applications.

ACS Biomater Sci Eng 2021 May 14. Epub 2021 May 14.

Environmental Science & Engineering, University of Texas at El Paso, El Paso, Texas 79968, United States.

Exosomes are cell-derived vesicles containing heterogeneous active biomolecules such as proteins, lipids, mRNAs, receptors, immune regulatory molecules, and nucleic acids. They typically range in size from 30 to 150 nm in diameter. An exosome's surfaces can be bioengineered with antibodies, fluorescent dye, peptides, and tailored for small molecule and large active biologics. Read More

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Affinity Series of Genetically Encoded Förster Resonance Energy-Transfer Sensors for Sucrose.

ACS Sens 2021 May 11. Epub 2021 May 11.

Institute for Molecular Physiology, Heinrich Heine University Düsseldorf, Düsseldorf 40225, Germany.

Genetically encoded fluorescent sugar sensors are valuable tools for the discovery of transporters and for quantitative monitoring of sugar steady-state levels in intact tissues. Genetically encoded Förster resonance energy-transfer sensors for glucose have been designed and optimized extensively, and a full series of affinity mutants is available for in vivo studies. However, to date, only a single improved sucrose sensor FLIPsuc-90μΔ1 with for sucrose of ∼90 μM was available. Read More

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A 1,8-naphthalimide-based lysosome-targeting dual-analyte fluorescent probe for the detection of pH and palladium in biological samples.

Talanta 2021 Aug 29;231:122365. Epub 2021 Mar 29.

Institute of Natural Medicine and Health Products, School of Life Science, Taizhou University, Taizhou, 318000, PR China; Department of Pharmaceutics, School of Pharmacy & Key Laboratory of Smart Drug Delivery, Fudan University, Shanghai, 200433, PR China. Electronic address:

Fluorescent probes containing 1,8-naphthalimide dyes have been used to detect biomolecules in the environmental and biological fields. However, most of the probes only exhibit single fluorescent output to one analyte, making them insufficient for detection of more analytes. Herein, we developed a novel 1,8-naphthalimide-based lysosome-targeting dual-analyte sensitive fluorescent probe (DPPP) for the detection of pH and palladium (Pd) using two different emissive channels. Read More

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Non-thermal plasma-induced DMPO-OH yields hydrogen peroxide.

Arch Biochem Biophys 2021 May 6;705:108901. Epub 2021 May 6.

Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Showa-Ku, Nagoya, 466-8550, Japan; Center for Low-temperature Plasma Sciences, Nagoya University, Chikusa-ku, Nagoya, 464-8603, Japan. Electronic address:

Recent developments in electronics have enabled the medical applications of non-thermal plasma (NTP), which elicits reactive oxygen species (ROS) and reactive nitrogen species (RNS), such as hydroxyl radical (OH), hydrogen peroxide (HO), singlet oxygen (O), superoxide (O), ozone, and nitric oxide at near-physiological temperatures. In preclinical studies or human clinical trials, NTP promotes blood coagulation, eradication of bacterial, viral and biofilm-related infections, wound healing, and cancer cell death. To elucidate the solution-phase biological effects of NTP in the presence of biocompatible reducing agents, we employed electron paramagnetic resonance (EPR) spectroscopy to quantify OH using a spin-trapping probe, 5,5-dimethyl-1-pyrroline-N-oxide (DMPO); O using a fluorescent probe; and O and HO using luminescent probes in the presence of thiols or tempol. Read More

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Fundamental photophysics of isomorphic and expanded fluorescent nucleoside analogues.

Chem Soc Rev 2021 May 6. Epub 2021 May 6.

Laboratoire de Bioimagerie et Pathologies, UMR 7021, Université de Strasbourg, 74 route du Rhin, 67401 Illkirch, France.

Fluorescent nucleoside analogues (FNAs) are structurally diverse mimics of the natural essentially non-fluorescent nucleosides which have found numerous applications in probing the structure and dynamics of nucleic acids as well as their interactions with various biomolecules. In order to minimize disturbance in the labelled nucleic acid sequences, the FNA chromophoric groups should resemble the natural nucleobases in size and hydrogen-bonding patterns. Isomorphic and expanded FNAs are the two groups that best meet the criteria of non-perturbing fluorescent labels for DNA and RNA. Read More

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A Compact Detection Platform Based on Gradient Guided-Mode Resonance for Colorimetric and Fluorescence Liquid Assay Detection.

Sensors (Basel) 2021 Apr 15;21(8). Epub 2021 Apr 15.

Department of Mechanical Engineering, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan.

This paper presents a compact spectral detection system for common fluorescent and colorimetric assays. This system includes a gradient grating period guided-mode resonance (GGP-GMR) filter and charge-coupled device. In its current form, the GGP-GMR filter, which has a size of less than 2. Read More

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Contribution of Glycation and Oxidative Stress to Thyroid Gland Pathology-A Pilot Study.

Biomolecules 2021 Apr 10;11(4). Epub 2021 Apr 10.

Department of Medical Biochemistry, Wroclaw Medical University, T. Chałubińskiego 10, 50-368 Wrocław, Poland.

The patho-mechanism of changes in the thyroid gland, including carcinogenesis, is a complex process, which involves oxidative stress. The goal of our investigation was to verify the extent of stress in the thyroid gland related to glycation. The study samples were comprised of blood sera, thyroid, and adipose tissue sections probed from 37 patients diagnosed with thyroid cancers and goiter. Read More

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Comparison of the Benzanthrone Luminophores: They Are Not Equal for Rapid Examination of (Trematoda: Digenea).

Biomolecules 2021 Apr 18;11(4). Epub 2021 Apr 18.

Institute of Life Sciences and Technology, Daugavpils University, Parades Street 1A, LV-5401 Daugavpils, Latvia.

Luminescent derivatives of benzanthrone are becoming more useful based on their light-absorbing and fluorescent-emitting properties. Our previous studies showed that luminescent staining properties of the same benzanthrone dye differ for variable parasite samples. Therefore, two types of benzanthrone dyes were prepared. Read More

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Metal-Modified Montmorillonite as Plasmonic Microstructure for Direct Protein Detection.

Sensors (Basel) 2021 Apr 9;21(8). Epub 2021 Apr 9.

Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomimetic Membranes and Textiles, Lerchenfeldstrasse 5, CH-9014 St. Gallen, Switzerland.

Thanks to its negative surface charge and high swelling behavior, montmorillonite (MMT) has been widely used to design hybrid materials for applications in metal ion adsorption, drug delivery, or antibacterial substrates. The changes in photophysical and photochemical properties observed when fluorophores interact with MMT make these hybrid materials attractive for designing novel optical sensors. Sensor technology is making huge strides forward, achieving high sensitivity and selectivity, but the fabrication of the sensing platform is often time-consuming and requires expensive chemicals and facilities. Read More

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Naphthalimide-Containing BP100 Leads to Higher Model Membranes Interactions and Antimicrobial Activity.

Biomolecules 2021 Apr 8;11(4). Epub 2021 Apr 8.

Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP 05508000, Brazil.

In a large variety of organisms, antimicrobial peptides (AMPs) are primary defenses against pathogens. BP100 (KKLFKKILKYL-NH), a short, synthetic, cationic AMP, is active against bacteria and displays low toxicity towards eukaryotic cells. BP100 acquires a α-helical conformation upon interaction with membranes and increases membrane permeability. Read More

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Simultaneously monitoring endogenous MAPK members in single living cells by multi-channel fluorescence correlation spectroscopy.

Analyst 2021 Apr;146(8):2581-2590

School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.

The mitogen-activated protein kinase (MAPK) pathway is a major module for cellular signal transduction. The dysregulation of the MAPK pathway has been involved in the pathogenesis of multiple diseases ranging from cancers to chronic inflammations. So far, we have not fully understood the influences of external factors and signaling networks on the MAPK pathway due to the lack of in situ methods for simultaneous detection of multiple kinases in the pathway in living cells. Read More

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Development of a one-step synthesized red emission fluorescent probe for sensitive detection of viscosity in vitro and in vivo.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Apr 8;258:119808. Epub 2021 Apr 8.

Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, PR China. Electronic address:

Diseases caused by metabolic abnormalities, such as inflammation and fatty liver, which are characterized by high viscosity, so it is necessary to detect the change of viscosity in vivo and in vitro. Due to the advantages of high sensitivity, noninvasive detection, high selectivity and real-time imaging, fluorescence imaging has become an effective means to detect biological parameters of biomolecules and life systems. Therefore, we have prepared a red emitting fluorescent probe NBI-V with easy synthesis which can ensure that the probe can be developed for the widely used to detection of viscosity changes in vivo and in vitro. Read More

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The molecular basis of spectral tuning in blue- and red-shifted flavin-binding fluorescent proteins.

J Biol Chem 2021 Apr 13:100662. Epub 2021 Apr 13.

Institut für Molekulare Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf, Forschungszentrum Jülich GmbH, D-52425 Jülich, Germany; IBG-1: Biotechnology: Forschungszentrum Jülich GmbH, D-52425 Jülich, Germany. Electronic address:

Photoactive biological systems modify the optical properties of their chromophores, known as spectral tuning. Determining the molecular origin of spectral tuning is instrumental for understanding function and developing applications of these biomolecules. Spectral tuning in flavin-binding fluorescent proteins (FbFPs), an emerging class of fluorescent reporters, is limited by their dependency on protein-bound flavins, whose structure and hence electronic properties cannot be altered by mutation. Read More

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A far-red hybrid voltage indicator enabled by bioorthogonal engineering of rhodopsin on live neurons.

Nat Chem 2021 May 15;13(5):472-479. Epub 2021 Apr 15.

College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of the Ministry of Education, Peking University, Beijing, China.

Membrane potential is a key aspect of cellular signalling and is dynamically regulated by an array of ion-selective pumps and channels. Fluorescent voltage indicators enable non-invasive optical recording of the cellular membrane potential with high spatial resolution. Here, we report a palette of bright and sensitive hybrid voltage indicators (HVIs) with fluorescence intensities sensitive to changes in membrane potential via electrochromic Förster resonance energy transfer. Read More

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Magneto-conducting multifunctional Janus microbots for intracellular delivery of biomolecules.

J Tissue Eng Regen Med 2021 Apr 13. Epub 2021 Apr 13.

Centre Biosystems Science and Engineering, Indian Institute of Science, Bengaluru, India.

Although several advances have been made in the field of medicine during the last few decades, yet targeted delivery of biomolecules is still a significant challenge. Thus, the present study illustrates the fabrication of dual nature magneto-conducting Fe O -SU8 derived carbon-based Janus microbots that could deliver biomolecules efficiently inside cells. These microsystems possess dual properties, that is, the half part is magneto-conducting, and another half is only conducting for sufficing the therapeutic payloads efficiently under electromagnetic stimulations. Read More

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Biodistribution of fluorescence-labelled EGF protein from slow release NanoZolid depots in mouse.

Int J Pharm 2021 May 15;601:120588. Epub 2021 Apr 15.

Experimental Cancer Medicine, Clinical Research Center, Karolinska Institutet, Stockholm, Sweden; Clinical Research Center (KFC) and Center for Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital Huddinge, Stockholm, Sweden. Electronic address:

Aim: The study was designed to evaluate the ability of the calcium sulfate based NanoZolid® drug delivery technology to locally release the epidermal growth factor (EGF) protein while maintaining its biological activity.

Methods: NanoZolid-formulated EGF protein labelled with a near infrared dye (EGF-NIR) depots or EGF-NIR dissolved in PBS were injected subcutaneously into mice bearing EGF receptor (EGFR) positive human A549 lung cancer tumors inoculated subcutaneously. The release and biodistribution of the EGF-NIR were investigated in vivo longitudinally up to 96 h post administration, utilizing whole body fluorescence imaging. Read More

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Graphene-Based Sensors for the Detection of Bioactive Compounds: A Review.

Int J Mol Sci 2021 Mar 24;22(7). Epub 2021 Mar 24.

Universidad de Alcalá, Facultad de Ciencias, Departamento de Química Analítica, Química Física e Ingeniería Química, Ctra. Madrid-Barcelona Km. 33.6, 28805 Alcalá de Henares, Madrid, España (Spain).

Over the last years, different nanomaterials have been investigated to design highly selective and sensitive sensors, reaching nano/picomolar concentrations of biomolecules, which is crucial for medical sciences and the healthcare industry in order to assess physiological and metabolic parameters. The discovery of graphene (G) has unexpectedly impulsed research on developing cost-effective electrode materials owed to its unique physical and chemical properties, including high specific surface area, elevated carrier mobility, exceptional electrical and thermal conductivity, strong stiffness and strength combined with flexibility and optical transparency. G and its derivatives, including graphene oxide (GO) and reduced graphene oxide (rGO), are becoming an important class of nanomaterials in the area of optical and electrochemical sensors. Read More

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Cytotoxicity of β-Cyclodextrins in Retinal Explants for Intravitreal Drug Formulations.

Molecules 2021 Mar 9;26(5). Epub 2021 Mar 9.

Faculty of Pharmaceutical Sciences, University of Iceland, Hofsvallagata 53, IS-107 Reykjavik, Iceland.

Cyclodextrins (CDs) have been widely used as pharmaceutical excipients for formulation purposes for different delivery systems. Recent studies have shown that CDs are able to form complexes with a variety of biomolecules, such as cholesterol. This has subsequently paved the way for the possibility of using CDs as drugs in certain retinal diseases, such as Stargardt disease and retinal artery occlusion, where CDs could absorb cholesterol lumps. Read More

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Influence of Dopamine on Fluorescent Advanced Glycation End Products Formation Using .

Biomolecules 2021 Mar 17;11(3). Epub 2021 Mar 17.

Department of Biotechnology, University of Rijeka, 51000 Rijeka, Croatia.

Non-enzymatic glycation and covalent modification of proteins leads to Advanced Glycation End products (AGEs). AGEs are biomarkers of aging and neurodegenerative disease, and can be induced by impaired neuronal signaling. The objective of this study was to investigate if manipulation of dopamine (DA) in vitro using the model protein, bovine serum albumin (BSA), and in vivo using the model organism influences fluorescent AGEs (fAGEs) formation as an indicator of dopamine-induced oxidation events. Read More

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Fluorescence spectroscopy as a novel technique for premarital screening of sickle cell disorders.

Photodiagnosis Photodyn Ther 2021 Mar 30;34:102276. Epub 2021 Mar 30.

Department of Urology, Cancer Research Chair, College of Medicine, King Saud University, Riyadh, 11451, Saudi Arabia.

Spectral diagnostic screening for sickle cell disease was carried out on volunteer blood samples (N = 100). The samples were subjected to different diagnostic methods including conventional complete blood count (CBC), hemoglobin electrophoresis (HBE) and spectral diagnosis. For the spectral diagnostic method, we discriminated three different characteristic spectral features. Read More

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Investigation of lipase-ligand interactions in porcine pancreatic extracts by microscale thermophoresis.

Anal Bioanal Chem 2021 Apr 2. Epub 2021 Apr 2.

Institut de Chimie Organique et Analytique (ICOA), CNRS FR 2708 - UMR 7311, Université d'Orléans, 45067, Orléans, France.

The evaluation of binding affinities between large biomolecules and small ligands is challenging and requires highly sensitive techniques. Microscale thermophoresis (MST) is an emerging biophysical technique used to overcome this limitation. This work describes the first MST binding method to evaluate binding affinities of small ligands to lipases from crude porcine pancreatic extracts. Read More

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Detection and Quantification of Tightly Bound Zn in Blood Serum Using a Photocaged Chelator and a DNAzyme Fluorescent Sensor.

Anal Chem 2021 04 31;93(14):5856-5861. Epub 2021 Mar 31.

Department of Chemistry, Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.

DNAzymes have emerged as a powerful class of sensors for metal ions due to their high selectivity over a wide range of metal ions, allowing for on-site and real-time detection. Despite much progress made in this area, detecting and quantifying tightly bound metal ions, such as those in the blood serum, remain a challenge because the DNAzyme sensors reported so far can detect only mobile metal ions that are accessible to bind the DNAzymes. To overcome this major limitation, we report the use of a photocaged chelator, XDPAdeCage to extract the Zn from the blood serum and then release the chelated Zn into a buffer using 365 nm light for quantification by an 8-17 DNAzyme sensor. Read More

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A potential role of green engineered TiO nanocatalyst towards enhanced photocatalytic and biomedical applications.

Environ Sci Pollut Res Int 2021 Mar 29. Epub 2021 Mar 29.

Department of Biotechnology, Periyar University, Salem, TN, 636011, India.

This study demonstrates a simple protocol for phytofabrication of titanium dioxide nanoparticles (TiONPs) wrapped with bioactive molecules from Ludwigia octovalvis leaf extract and their characterization by UV-visible absorption spectroscopy, Fourier transform spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), X-ray photoelectron spectrum (XPS), and diffuse reflectance spectrum (DRS). The bandgap energy of pure green engineered TiO nanoparticles was determined by DRS analysis. The XPS analysis confirmed the purity of the TiO nanoparticles. Read More

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Nanoscale Visualization of Morphological Alteration of Live-Cell Membranes by the Interaction with Oligoarginine Cell-Penetrating Peptides.

Anal Chem 2021 04 26;93(13):5383-5393. Epub 2021 Mar 26.

Graduate School of Environmental Studies, Tohoku University, Sendai, Miyagi 980-8579, Japan.

The interactions between the cell membrane and biomolecules remain poorly understood. For example, arginine-rich cell-penetrating peptides (CPPs), including octaarginines (R8), are internalized by interactions with cell membranes. However, during the internalization process, the exact membrane dynamics introduced by these CPPs are still unknown. Read More

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An Approach for the Real-Time Quantification of Cytosolic Protein-Protein Interactions in Living Cells.

ACS Sens 2021 04 24;6(4):1572-1582. Epub 2021 Mar 24.

Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, 8092 Zurich, Switzerland.

In recent years, cell-based assays have been frequently used in molecular interaction analysis. Cell-based assays complement traditional biochemical and biophysical methods, as they allow for molecular interaction analysis, mode of action studies, and even drug screening processes to be performed under physiologically relevant conditions. In most cellular assays, biomolecules are usually labeled to achieve specificity. Read More

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Engineered Regulon to Enable Autonomous Azide Ion Biosensing, Recombinant Protein Production, and Glycoengineering.

ACS Synth Biol 2021 04 22;10(4):682-689. Epub 2021 Mar 22.

Department of Chemical & Biochemical Engineering, Rutgers, The State University of New Jersey, 98 Brett Road, Piscataway, New Jersey 08854, United States.

Detection of azide-tagged biomolecules (, azido sugars) inside living cells using "click" chemistry has been revolutionary to the field of chemical biology. However, we currently still lack suitable synthetic biology tools to autonomously and rapidly detect azide ions. Here, we have developed an engineered synthetic promoter system called regulon, and complementary engineered strains, to selectively detect azide ions and autonomously induce downstream expression of reporter genes. Read More

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Progress in Tuning Emission of the Excited-State Intramolecular Proton Transfer (ESIPT)-Based Fluorescent Probes.

ACS Omega 2021 Mar 4;6(10):6547-6553. Epub 2021 Mar 4.

Department of Chemistry, University of Akron, Akron, Ohio 44325, United States.

In this review, we will summarize our recent progress in the design and application of novel organic sensors with emission in the near-infrared region (600-900 nm). By coupling different functional groups with excited-state intramolecular proton transfer (ESIPT) segments, new probes are developed to achieve a large Stokes shift, high sensitivity, and selectivity and to tune the emission toward the near-infrared region. The developed probes exhibit attractive optical properties for bioimaging and environmental science applications. Read More

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A crosslinked submicro-hydrogel formed by DNA circuit-driven protein aggregation amplified fluorescence anisotropy for biomolecules detection.

Anal Chim Acta 2021 Apr 12;1154:338319. Epub 2021 Feb 12.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, 400715, Chongqing, PR China. Electronic address:

Protein is an excellent molecular mass amplifier without fluorescence quenching effect for fluorescence anisotropy (FA) assay. However, in traditional protein amplified FA methods, the binding ratio between amplifier and dye-modified probe is 1:1 or one target can only induce FA change of one fluorophore on probe, resulting in low sensitivity. Herein, we developed a simple FA strategy with high accuracy and sensitivity by using a crosslinked submicro-hydrogel that was formed through a catalyzed hairpin assembly (CHA) assisted protein aggregation as a novel FA amplifier. Read More

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Uptake and Intracellular Trafficking Studies of Multiple Dye-Doped Core-Shell Silica Nanoparticles in Lymphoid and Myeloid Cells.

Nanotechnol Sci Appl 2021 8;14:29-48. Epub 2021 Mar 8.

Department of Biomolecular Sciences, University of Urbino Carlo Bo, Urbino, PU, 61029, Italy.

Introduction: Since most biologically active macromolecules are natural nanostructures, operating in the same scale of biomolecules gives the great advantage to enhance the interaction with cellular components. Noteworthy efforts in nanotechnology, particularly in biomedical and pharmaceutical fields, have propelled a high number of studies on the biological effects of nanomaterials. Moreover, the determination of specific physicochemical properties of nanomaterials is crucial for the evaluation and design of novel safe and efficient therapeutics and diagnostic tools. Read More

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