15,523 results match your criteria biomass enzyme

Dynamic modeling of the activated sludge microbial growth and activity under exposure to heavy metals.

Bioresour Technol 2021 Jul 21;339:125623. Epub 2021 Jul 21.

Wroclaw University of Science and Technology, Faculty of Chemistry, Department of Micro, Nano, and Bioprocess Engineering, Norwida 4/6, 50-373 Wrocław, Poland.

The presence of heavy metals in the environment can lead to ecological and health problems. The evolution of biological systems, such as activated sludge, exposed to heavy metals is still underexplored. Therefore, this study sought to develop a model of microorganism activity and growth in activated sludge and used it to investigate the toxicity of five metals: Cu, Cd, Ni, Zn, and Ag. Read More

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Assessing the arsenic-saturated biochar recycling potential of vermitechnology: Insights on nutrient recovery, metal benignity, and microbial activity.

Chemosphere 2021 Jul 23;286(Pt 1):131660. Epub 2021 Jul 23.

Agricultural and Ecological Research Unit, Indian Statistical Institute, Giridih, Jharkhand, 815301, India. Electronic address:

Biochar mediated pollutant removal is gaining attention because of high efficiency of the process. However, effective recycling avenues of the pollutant-saturated biochars are scarce in the knowledge base; while such materials can be a new source of long-range contamination. Therefore, potential of vermitechnology for eco-friendly recycling of pollutant-loaded biochar was assessed by using arsenic-saturated native (NBC) and exfoliated (EBC) biochars as feedstocks for the first time. Read More

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Cellulolytic enzymes production guided by morphology engineering.

Enzyme Microb Technol 2021 Sep 26;149:109833. Epub 2021 May 26.

Purdue University, West Lafayette, IN, 47907-2032, United States. Electronic address:

Endoglucanase and xylanase are critical enzymes for liquefaction and enzyme hydrolysis of high solids lignocellulosic biomass to facilitate its transport and production of desired derived products. Here is reported how combinations of different spore concentrations and pH influence microbial morphology, and how this may be used to direct expression and secretion of enzymes by Aspergillus niger. While xylanase production is not affected by A. Read More

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September 2021

Use of green light to improve the production of lignocellulose-decay enzymes by Pleurotus spp. in liquid cultivation.

Enzyme Microb Technol 2021 Sep 25;149:109860. Epub 2021 Jun 25.

Graduate Program in Biotechnology Applied to Agriculture, Paranaense University, Praça Mascarenhas de Moraes, 4282, Umuarama, PR, Brazil. Electronic address:

The influence of green light on mycelium biomass growth and extracellular enzyme activities of edible mushrooms from the Pleurotus genus, which is popularly cultivated all over the world, were investigated. The mycelium of seven strains of five species of Pleurotus (P. citrinopileatus, P. Read More

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September 2021

Anthocyanin extract from Lycium ruthenicum enhanced production of biomass and polysaccharides during submerged fermentation of Agaricus bitorquis (Quél.) Sacc. Chaidam.

Bioprocess Biosyst Eng 2021 Jul 23. Epub 2021 Jul 23.

College of Agriculture and Animal Husbandry, Qinghai University, No.251 Ningda Road, Xining, 810016, Qinghai, China.

Agaricus bitorquis (Quél.) Sacc. Chaidam (ABSC) is a wild edible fungus uniquely found in the Tibet Plateau. Read More

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Terricolous mosses impact soil microbial biomass carbon and enzymatic activity under temperate forest types of the Garhwal Himalayas.

Environ Monit Assess 2021 Jul 26;193(8):516. Epub 2021 Jul 26.

Ecology and Ecosystem Research Laboratory, Department of Botany, University of Delhi, Delhi, 110007, India.

Estimates of enzymatic activity are used as indices for soil quality, microbial nutrient demand, microbial growth, and activity. Mosses trap soil moisture, influence soil temperature, and create a microenvironment promoting an overall higher level of microbial activity, thus making the decomposition of organic matter more favorable. This study determines the role of mosses in influencing soil biochemical properties in three temperate forest types of the Garhwal Himalayas, Uttarakhand, viz. Read More

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Enhanced Production of (S)-2-arylpropionic Acids by Protein Engineering and Whole-Cell Catalysis.

Front Bioeng Biotechnol 2021 7;9:697677. Epub 2021 Jul 7.

Hefei National Laboratory for Physical Sciences at the Microscale, CAS Key Laboratory of Urban Pollutant Conversion, Anhui Province Key Laboratory of Biomass Clean Energy, iChEM, University of Science and Technology of China, Hefei, China.

Esterases are important biocatalysts for chemical synthesis. Several bHSL family esterases have been used to prepare ()-2-arylpropionic acids with stronger anti-inflammatory effects kinetic resolution. Here, we presented the discovery of key residues that controlled the enantioselectivity of bHSL family esterases to ethyl 2-arylpropionates, through careful analysis of the structural information and molecular docking. Read More

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Combined effects of biochar and chicken manure on maize ( L.) growth, lead uptake and soil enzyme activities under lead stress.

PeerJ 2021 12;9:e11754. Epub 2021 Jul 12.

College of Agriculture, Henan University of Science and Technology, Luoyang, Henan, China.

The goal of the present work was to evaluate the additive effects of biochar and chicken manure on maize growth in Pb-contaminated soils. In this study, we conducted a pot experiment to investigate how biochar in soil (20, 40 g·kg), chicken manure in soil (20, 40 g·kg), or a combination of biochar and chicken manure in soil (each at 20 g·kg) effect maize growth, Pb uptake, leaves' antioxidant enzymatic activities, and soil enzyme activities under artificial conditions to simulate moderate soil pollution (800 Pb mg·kg). The results showed that all biochar and/or chicken manure treatments significantly ( < 0. Read More

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Valorisation of biomass and diaper waste into a sustainable production of the medical mushroom Lingzhi Ganoderma lucidum.

Chemosphere 2021 Jul 10;286(Pt 1):131477. Epub 2021 Jul 10.

Henan Province International Collaboration Lab of Forest Resources Utilization, School of Forestry, Henan Agricultural University, Zhengzhou, 450002, China; Department of Bioscience, Aarhus University, Arctic Research Center (ARC), Frederiksborgvej 399, PO box 358, DK- 4000, Roskilde, Denmark. Electronic address:

Global solid waste is expected to increase by at least 70% annually until year 2050. The mixture of solid waste including food waste from food industry and domestic diaper waste in landfills is causing environmental and human health issues. Nevertheless, food and diaper waste containing high lignocellulose can easily degrade using lignocellulolytic enzymes thereby converted into energy for the development and growth of mushroom. Read More

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Enhancement of salt tolerance in corn using Azospirillum brasilense: an approach on antioxidant systems.

J Plant Res 2021 Jul 24. Epub 2021 Jul 24.

Universidade Estadual Paulista (UNESP), Faculdade de Ciências Agrárias e Veterinárias, Depto. de Biologia Aplicada à Agropecuária, Jaboticabal, SP, 14884-900, Brazil.

Salinity has become one of the major factors limiting agricultural production. In this regard, different cost-effective management strategies such as the use of plant growth-promoting bacteria (PGPB) as inoculants to alleviate salt-stress conditions and minimize plant productivity losses have been used in agricultural systems. The aim of this study was to characterize induced antioxidant responses in corn through inoculation with Azospirillum brasilense and examine the relationship between these responses and the acquired salt-stress tolerance. Read More

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Anaerobic hydrolysis of complex substrates in full-scale aerobic granular sludge: enzymatic activity determined in different sludge fractions.

Appl Microbiol Biotechnol 2021 Jul 24. Epub 2021 Jul 24.

Section Sanitary Engineering, Department of Water Management, Delft University of Technology, Stevinweg 1, Delft, 2628 CN, The Netherlands.

Complex substrates, like proteins, carbohydrates, and lipids, are major components of domestic wastewater, and yet their degradation in biofilm-based wastewater treatment technologies, such as aerobic granular sludge (AGS), is not well understood. Hydrolysis is considered the rate-limiting step in the bioconversion of complex substrates, and as such, it will impact the utilization of a large wastewater COD (chemical oxygen demand) fraction by the biofilms or granules. To study the hydrolysis of complex substrates within these types of biomass, this paper investigates the anaerobic activity of major hydrolytic enzymes in the different sludge fractions of a full-scale AGS reactor. Read More

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Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping.

Biotechnol Biofuels 2021 Jul 23;14(1):162. Epub 2021 Jul 23.

School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an, 710049, China.

Background: As one of the major components of lignocellulosic biomass, lignin has been considered as the most abundant renewable aromatic feedstock in the world. Comparing with thermal or catalytic strategies for lignin degradation, biological conversion is a promising approach featuring with mild conditions and diversity, and has received great attention nowadays.

Results: In this study, a consortium of white rot fungi composed of Lenzites betulina and Trametes versicolor was employed to enhance the ligninolytic enzyme activity of laccase (Lac) and manganese peroxidase (MnP) under microbial synergism. Read More

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Enzymatic utilization of oil and lignocellulosic biomass using halophilic marine bacteria and .

3 Biotech 2021 Jul 1;11(7):360. Epub 2021 Jul 1.

Department of Environmental Engineering, College of Engineering, Ajou University, Suwon, Gyeonggi-do South Korea.

In this study, hydrolytic and oxidative activities of enzymes isolated from halophilic microbes were characterized and applied for biomass utilization. First, lipase from , and peroxidase and laccase from and were selected and their catalytic activities were determined, respectively. The lipase encoding gene was synthesized after codon-optimization and could be successfully expressed in with the assist of the Tif chaperone protein. Read More

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Hybrid Genome Assembly for Predicting Functional Potential of a Novel Strain as Plant Biomass Valorisation Agent.

Indian J Microbiol 2021 Sep 22;61(3):283-290. Epub 2021 Apr 22.

NUS Environmental Research Institute, National University of Singapore, Singapore, 117411 Singapore.

Environmental bioremediation relies heavily on the realized potential of efficient bioremediation agents or microbial strains of interest. Identifying suitable microbial agents for plant biomass waste valorization requires (i) high-quality genome assemblies to predict the full metabolic and functional potential, (ii) accurate mapping of lignocellulose metabolizing enzymes. However, fragmented nature of the sequenced genomes often limits the prediction ability due to breaks occurring in coding sequences. Read More

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September 2021

Economizing the lignocellulosic hydrolysis process using heterologously expressed auxiliary enzymes feruloyl esterase D (CE1) and β-xylosidase (GH43) derived from thermophilic fungi Scytalidium thermophilum.

Bioresour Technol 2021 Jul 17;339:125603. Epub 2021 Jul 17.

Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab-143005, India. Electronic address:

Two lignocellulolytic accessory enzymes, feruloyl esterase D (FAED_SCYTH) and β-xylosidase (XYL43B_SCYTH) were cloned and produced in the Pichia pastoris X33 as host. The molecular weight of recombinant enzymes FAED_SCYTH and XYL43B_SCYTH were ~ 31 and 40 kDa, respectively. FAED_SCYTH showed optimal activity at pH 6. Read More

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Nitrogenous Compound Utilization and Production of Volatile Organic Compounds among Commercial Wine Yeasts Highlight Strain-Specific Metabolic Diversity.

Microbiol Spectr 2021 Jul 21:e0048521. Epub 2021 Jul 21.

Food Microbiology, Wageningen University & Research, Wageningen, The Netherlands.

Genetic background and environmental conditions affect the production of sensory impact compounds by Saccharomyces cerevisiae. The relative importance of the strain-specific metabolic capabilities for the production of volatile organic compounds (VOCs) remains unclear. We investigated which amino acids contribute to VOC production and whether amino acid-VOC relations are conserved among yeast strains. Read More

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Exogenous salicylic acid mediated herbicide (Paraquat) resistance in cyanobacterial biofertilizer Microchaete sp. NCCU-342.

Environ Sci Pollut Res Int 2021 Jul 20. Epub 2021 Jul 20.

Cyanobacterial Biotechnology Laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi, India.

Paddy field farming remains the dominant form of growing rice in modern times as the rice is the staple food for over half the world's population and is closely associated with food security and political stability of many countries. Record increase in rice production have been observed since the start of the Green Revolution. India is one of the largest paddy producer and exporter in the world. Read More

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Cleaving arene rings for acyclic alkenylnitrile synthesis.

Nature 2021 Jul 19. Epub 2021 Jul 19.

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.

Synthetic chemistry is built around the formation of carbon-carbon bonds. Conversely, selective methods for C-C bond cleavage is a largely unmet challenge, the solution of which will provide promising applications in synthesis, coal liquefaction, petroleum cracking, polymer degradation and biomass conversion. For example, aromatic rings are ubiquitous skeletal features in inert chemical feed stocks, but are inert to many reaction conditions owing to their aromaticity and low polarity. Read More

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Critical insights into psychrophilic anaerobic digestion: Novel strategies for improving biogas production.

Waste Manag 2021 Jul 16;131:513-526. Epub 2021 Jul 16.

Global Institute for Energy, Environment and Sustainability, Lenexa, USA.

Anaerobic digestion (AD) under psychrophilic temperature has only recently garnered deserved attention. In major parts of Europe, USA, Canada and Australia, climatic conditions are more suited for psychrophilic (<20 ℃) rather than mesophilic (35 - 37 ℃) and thermophilic (55 - 60 ℃) AD. Low temperature has adverse effects on important cellular processes which may render the cell biology inactive. Read More

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Catalase Activity is Critical for Biofilm Development, EPS Composition, and Dissemination During Catheter-Associated Urinary Tract Infection.

Infect Immun 2021 Jul 19:IAI0017721. Epub 2021 Jul 19.

Department of Microbiology and Immunology, Jacobs School of Medicine and Biomedical Sciences, State University of New York at Buffalo.

is a leading uropathogen of catheter-associated urinary tract infections (CAUTIs), which are among the most common healthcare-associated infections worldwide. A key factor that contributes to pathogenesis and persistence during CAUTI is the formation of catheter biofilms, which provide increased resistance to antibiotic treatment and host defense mechanisms. Another factor that is important for bacterial persistence during CAUTI is the ability to resist reactive oxygen species (ROS), such as through the action of the catalase enzyme. Read More

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Response and tolerance ability of to cadmium pollution stress.

Environ Technol 2021 Jul 18:1-11. Epub 2021 Jul 18.

College of Safety and Environment Engineering, Shandong University of Science and Technology, Qingdao, People's Republic of China.

Cadmium, which is widely used in electroplating industry, chemical industry, electronic industry and nuclear industry, is harmful to human health and ecological environment. The effects of Cd at different initial concentrations on biomass, antioxidant enzyme activity and ultrastructure of were analysed in the present study. The results showed that maintained a slow-growth trend at 3. Read More

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Mycorrhizal-Assisted Phytoremediation and Intercropping Strategies Improved the Health of Contaminated Soil in a Peri-Urban Area.

Front Plant Sci 2021 2;12:693044. Epub 2021 Jul 2.

Department of Plant Biology and Ecology, University of the Basque Country (UPV/EHU), Leioa, Spain.

Soils of abandoned and vacant lands in the periphery of cities are frequently subjected to illegal dumping and can undergo degradation processes such as depletion of organic matter and nutrients, reduced biodiversity, and the presence of contaminants, which may exert an intense abiotic stress on biological communities. Mycorrhizal-assisted phytoremediation and intercropping strategies are highly suitable options for remediation of these sites. A two-year field experiment was conducted at a peri-urban site contaminated with petroleum hydrocarbons and polychlorinated biphenyls, to assess the effects of plant growth (spontaneous plant species, , and × , alone intercropped) and inoculation of a commercial arbuscular mycorrhizal and ectomycorrhizal inoculum. Read More

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Cellulosic biofuel production using emulsified simultaneous saccharification and fermentation (eSSF) with conventional and thermotolerant yeasts.

Biotechnol Biofuels 2021 Jul 17;14(1):157. Epub 2021 Jul 17.

Department of Chemical and Biological Engineering, Hoyt Laboratory, Princeton University, 101 Hoyt Laboratory, William Street, Princeton, NJ, 08544, USA.

Background: Future expansion of corn-derived ethanol raises concerns of sustainability and competition with the food industry. Therefore, cellulosic biofuels derived from agricultural waste and dedicated energy crops are necessary. To date, slow and incomplete saccharification as well as high enzyme costs have hindered the economic viability of cellulosic biofuels, and while approaches like simultaneous saccharification and fermentation (SSF) and the use of thermotolerant microorganisms can enhance production, further improvements are needed. Read More

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Root cell wall chemistry remodelling enhanced arsenic fixation of a cabbage cultivar.

J Hazard Mater 2021 May 20;420:126165. Epub 2021 May 20.

Guangdong Provincial Research Centre for Environment Pollution Control and Remediation Materials, Department of Ecology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. Electronic address:

The low- and high-arsenic (As) transferring cultivars (LTC and HTC) of cabbage showed significant differences in As uptake and distribution. We hypothesise that chemistry of root cell wall matrix plays a critical role. LTC and HTC were treated with As and grown for 60 days. Read More

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C:N:P stoichiometry responses to 10 years of nitrogen addition differ across soil components and plant organs in a subtropical Pleioblastus amarus forest.

Sci Total Environ 2021 Jul 8;796:148925. Epub 2021 Jul 8.

Key Laboratory of National Forestry, Grassland Administration on Forest Resources Conservation and Ecological Safety in the Upper Reaches of the Yangtze River, College of Forestry, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Electronic address:

How stoichiometry in different ecosystem components responds to long-term nitrogen (N) addition is crucial for understanding within-ecosystem biogeochemistry cycling processes in the context of global change. To explore the effects of long-term N addition on nutrient stoichiometry in soil and plant components in forest ecosystem, a 10-year N addition experiment using ammonium nitrate (NHNO) was conducted in a bamboo forest in the Rainy Zone of West China, where the background N deposition is the highest in the world. Four N treatment levels (+0, +50, +150, +300 kg N ha yr) (CK, LN, MN, HN) were applied monthly since November 2007, and then, the C:N:P stoichiometry of soil, microbial biomass, and enzymes in rhizosphere soil and bulk soil, and plant organs were measured. Read More

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Efficiency enhancement of a new cellulase cocktail at low enzyme loading for high solid digestion of alkali catalyzed atmospheric glycerol organosolvent pre-treated sugarcane bagasse.

Bioresour Technol 2021 Jul 10;338:125505. Epub 2021 Jul 10.

School of Life Sciences, University of Science and Technology of China, Hefei, China.

The acquisition during biomass saccharification of elevated levels of fermentable sugars with lower cellulase concentration is central to ensuring an economically viable and industrially relevant hydrolytic process. Thus, using a new cellulase preparation (LT4) at low cellulase loading (2 mg protein/g dried substrate), this study assessed the possible boosting effect of integrating accessory enzymes and additives on high-solids hydrolysis of sugarcane bagasse via fed-batch feeding. Hydrolysis which commenced with initial 8% solids loading and subsequent substrate feeding of 4% solids at 6 h, 18 h, and 24 h respectively, proved optimal for the 20% high-solids saccharification producing 158 g/L total sugars and 83% glucose yield after 72 h with the combined optimized additives and accessory enzymes. Read More

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Novel catalytic potential of a hyperthermostable mono‑copper oxidase (LPMO-AOAA17) for the oxidation of lignin monomers and depolymerisation of lignin dimer in aqueous media.

Int J Biol Macromol 2021 Jul 14;186:563-573. Epub 2021 Jul 14.

Center of Innovative and Applied Bioprocessing (CIAB), Knowledge City, Sector-81, Mohali 140306, Punjab, India. Electronic address:

Lytic polysaccharide monooxygenase (LPMO) are mono‑copper enzymes known for the oxidative cleavage of recalcitrant polysaccharides with their intriguing and unique catalytic chemistry. Such impeccable oxidation potential has made them highly valuable in the enzymatic consortia for the degradation of ligno-cellulosic biomass. Bioinformatic analysis has revealed an unannotated LPMO gene in the genome of A. Read More

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Efficient pullulan production by Aureobasidium pullulans using cost-effective substrates.

Int J Biol Macromol 2021 Jul 14;186:544-553. Epub 2021 Jul 14.

School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, PR China. Electronic address:

In this study, cost-effective substrates such as cassava starch, corn steep liquor (CSL) and soybean meal hydrolysate (SMH) were used for pullulan production by Aureobasidium pullulans CCTCC M 2012259. The medium was optimized using response surface methodology (RSM) and artificial neural network (ANN) approaches, and analysis of variance indicated that the ANN model achieved higher prediction accuracy. The optimal medium predicted by ANN was used to produce high molecular weight pullulan in high yield. Read More

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Phytostabilization of a contaminated military site using miscanthus and soil amendments.

J Environ Qual 2021 Jul 17. Epub 2021 Jul 17.

Department of Environmental Chemistry and Technology, J.E. Purkyně University, Ústí nad Labem, 40096, Czech Republic.

Military activities can contaminate productive land with potentially toxic substances. The most common trace metal contaminant on military bases is lead (Pb). A field experiment was begun in 2016 at Fort Riley, KS, in an area with total soil Pb concentrations ranging from 900 to 1,500 mg kg and near-neutral pH. Read More

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Salicylic acid underpins silicon in ameliorating chromium toxicity in rice by modulating antioxidant defense, ion homeostasis and cellular ultrastructure.

Plant Physiol Biochem 2021 Jul 12;166:1001-1013. Epub 2021 Jul 12.

Institute of Crop Science, Ministry of Agriculture and Rural Affairs Laboratory of Spectroscopy Sensing, Zhejiang University, Hangzhou, 310058, China. Electronic address:

Chromium (Cr) phytotoxicity affirmed the need of mitigation strategies to remediate polluted soils and restricts its accumulation in the food chains. Salicylic acid (SA) and silicon (Si) play pivotal roles in stimulating the plant performance and stress resilience. So far, their interactive effects against Cr-phytotoxicities are less known. Read More

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