809 results match your criteria bioluminescent signal

A lasting symbiosis: how Vibrio fischeri finds a squid partner and persists within its natural host.

Nat Rev Microbiol 2021 Jun 4. Epub 2021 Jun 4.

Pacific Biosciences Research Center, Kewalo Marine Laboratory, University of Hawai'i at Manoa, Honolulu, HI, USA.

As our understanding of the human microbiome progresses, so does the need for natural experimental animal models that promote a mechanistic understanding of beneficial microorganism-host interactions. Years of research into the exclusive symbiosis between the Hawaiian bobtail squid, Euprymna scolopes, and the bioluminescent bacterium Vibrio fischeri have permitted a detailed understanding of those bacterial genes underlying signal exchange and rhythmic activities that result in a persistent, beneficial association, as well as glimpses into the evolution of symbiotic competence. Migrating from the ambient seawater to regions deep inside the light-emitting organ of the squid, V. Read More

View Article and Full-Text PDF

Bioluminescence Imaging of Neuronal Network Dynamics Using Aequorin-Based Calcium Sensors.

Methods Mol Biol 2021 ;2274:281-294

Neuroscience Paris Seine-Institut de Biologie Paris Seine (NPS-IBPS), Sorbonne Université UM119, CNRS UMR8246, INSERM U1130, Paris, France.

Optogenetic calcium sensors enable the imaging in real-time of the activities of single or multiple neurons in brain slices and in vivo. Bioluminescent probes engineered from the natural calcium sensor aequorin do not require illumination, are virtually devoid of background signal, and exhibit wide dynamic range and low cytotoxicity. These probes are thus well suited for long-duration, whole-field recordings of multiple neurons simultaneously. Read More

View Article and Full-Text PDF
January 2021

A Real-Time, Bioluminescent Apoptosis Assay.

Methods Mol Biol 2021 ;2255:55-67

Promega, Madison, WI, USA.

This chapter describes a real-time, bioluminescent apoptosis assay technique, which circumvents the well-documented "timing condundrum" encountered when employing traditional apoptosis detection chemistries after exposures with inducers of unknown potential. The assay continuously reports the translocation of phosphatidylserine (PS) from the inner membrane leaflet of a cell to the exofacial surface during apoptosis. This homogenous, no-wash, plate-based assay is made possible by two different annexin V fusion proteins, which contain complementing NanoBiT™ luciferase enzyme subunits, a time-released luciferase substrate, and a fluorescent membrane integrity reagent. Read More

View Article and Full-Text PDF
January 2021

Bacterial bioreporters for the detection of trace explosives: performance enhancement by DNA shuffling and random mutagenesis.

Appl Microbiol Biotechnol 2021 May 4;105(10):4329-4337. Epub 2021 May 4.

Department of Plant & Environmental Sciences, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.

Landmines and other explosive remnants of war pose a global humanitarian problem that claims numerous casualties long after the conflict has ended. As there are no acceptable methodologies for the remote discovery of such devices, current detection practices still require the risky presence of personnel in the minefield. We have recently described bacterial sensor strains capable of reporting the existence of 2,4-dinitrotoluene (DNT) vapors in the soil above 2,4,6-trinitrotoluene (TNT)-based landmines, by generating a bioluminescent or a fluorescent signal. Read More

View Article and Full-Text PDF

Model-based evaluation of image-guided fractionated whole-brain radiation therapy in pediatric diffuse intrinsic pontine glioma xenografts.

CPT Pharmacometrics Syst Pharmacol 2021 May 3. Epub 2021 May 3.

Department of Pharmaceutical Sciences, St. Jude Children's Research Hospital, Memphis, TN, USA.

Radiation therapy (RT) is currently the standard treatment for diffuse intrinsic pontine glioma (DIPG), the most common cause of death in children with brain cancer. A pharmacodynamic model was developed to describe the radiation-induced tumor shrinkage and overall survival in mice bearing DIPG. CD1-nude mice were implanted in the brain cortex with luciferase-labeled patient-derived orthotopic xenografts of DIPG (SJDIPGx7 H3F3A and SJDIPGx37 H3F3A ). Read More

View Article and Full-Text PDF

An autonomous bioluminescent bacterial biosensor module for outdoor sensor networks, and its application for the detection of buried explosives.

Biosens Bioelectron 2021 Aug 15;185:113253. Epub 2021 Apr 15.

Department of Plant & Environmental Sciences, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, 9190401, Israel.

We describe a miniaturized field-deployable biosensor module, designed to function as an element in a sensor network for standoff monitoring and mapping of environmental hazards. The module harbors live bacterial sensor cells, genetically engineered to emit a bioluminescent signal in the presence of preselected target materials, which act as its core sensing elements. The module, which detects and processes the biological signal, composes a digital record that describes its findings, and can be transmitted to a remote receiver. Read More

View Article and Full-Text PDF

Development of a CRISPR-Cas9 Based Luciferase Turn-On System as Nonhomologous End Joining Pathway Reporter.

Chembiochem 2021 Jun 7;22(12):2177-2181. Epub 2021 May 7.

Department of Breast Cancer Pathology and Research Laboratory, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin's Clinical Research Center for Cancer, Tianjin, 300060, P. R. China.

There is a need of a non-homologous end joining (NHEJ) pathway reporter system that facilitates screening and discovery of NHEJ chemical inhibitors. In this study, we developed a CRISPR-Cas9 based luciferase turn-on system as a NHEJ pathway reporter. By substituting nucleotide 205C with ATC, we introduced a reading-frame shift and a pre-stop codon into the luciferase coding region and thereby generated a bioluminescent signal mute HEK293T reporter cell line. Read More

View Article and Full-Text PDF

Bioluminescent Tumor Signal Is Mouse Strain and Pelt Color Dependent: Experience in a Disseminated Lymphoma Model.

Mol Imaging Biol 2021 Apr 8. Epub 2021 Apr 8.

Mallinckrodt Institute of Radiology, Washington University School of Medicine, Campus Box 8131, 510 South Kingshighway Boulevard, St Louis, MO, 63110, USA.

Background: Many preclinical cancer studies use mice with varied phenotypes to monitor tumor treatment. We compared survival and optical imaging characteristics of strains with varied coat colors harboring luciferase-expressing disseminated lymphoma.

Results: Luciferase-expressing lymphoma cells (Raji-luc) were injected via tail vein into severe combined immunodeficient (SCID) and Rag2-IL2rg (R2G2) mice, and survival was tracked. Read More

View Article and Full-Text PDF

COR758, a negative allosteric modulator of GABA receptors.

Neuropharmacology 2021 05 30;189:108537. Epub 2021 Mar 30.

Department of Biomedical Sciences, University of Cagliari, 09042, Monserrato, Italy; Guy Everett Laboratory, University of Cagliari, 09042, Monserrato, Italy; Center of Excellence "Neurobiology of Addiction", University of Cagliari, 09042, Monserrato, Italy. Electronic address:

Allosteric modulators of G protein coupled receptors (GPCRs), including GABARs (GABARs), are promising therapeutic candidates. While several positive allosteric modulators (PAM) of GABARs have been characterized, only recently the first negative allosteric modulator (NAM) has been described. In the present study, we report the characterization of COR758, which acts as GABAR NAM in rat cortical membranes and CHO cells stably expressing GABARs (CHO-GABA). Read More

View Article and Full-Text PDF

A novel bioluminescent approach to the loop-mediated isothermal amplification-based detection of Lactobacillus salivarius in feed samples.

J Microbiol Methods 2021 Mar 23:106209. Epub 2021 Mar 23.

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China. Electronic address:

Coupling loop-mediated isothermal amplification (LAMP) with a bioluminescent assay in real-time (LAMP-BART) is a strategy that can be readily leveraged to detect bacteria in particular samples of interest without the need for costly or complicated equipments. However, this approach exhibits poor sensitivity, and it additionally amplifies all target DNA including that derived from non-viable cells. Herein, we sought to overcome these traditional pyrophosphate bioluminescent assay limitations by utilizing 2-deoxyadenosine-5-(α-thio) -triphosphate (dATPαS) in place of dATP when conducting LAMP, thereby markedly reducing and stabilizing overall background signal levels, resulting in a detection limit of 3 CFU/μL. Read More

View Article and Full-Text PDF

The G1 phase optical reporter serves as a sensor of CDK4/6 inhibition .

Int J Biol Sci 2021 31;17(3):728-741. Epub 2021 Jan 31.

Cancer Center & Department of Breast and Thyroid Surgery, Xiang'an Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, Fujian, China.

Visualization of cell-cycle G1 phase for monitoring the early response of cell cycle specific drug remains challenging. In this study, we developed genetically engineered bioluminescent reporters by fusing full-length cyclin E to the C-terminal luciferase (named as CycE-Luc and CycE-Luc2). Next, HeLa cell line or an ER-positive breast cancer cell line MCF-7 was transfected with these reporters. Read More

View Article and Full-Text PDF
January 2021

Equilibrium and Kinetic Measurements of Ligand Binding to HiBiT-tagged GPCRs on the Surface of Living Cells.

Bio Protoc 2020 Dec 20;10(24):e3861. Epub 2020 Dec 20.

Promega Corporation, Fitchburg, Wisconsin, USA.

G-protein coupled receptors (GPCRs) remain at the forefront of drug discovery efforts. Detailed assessment of features contributing to GPCR ligand engagement in a physiologically relevant environment is imperative to the development of new therapeutics with improved efficacy. Traditionally, binding properties such as affinity and kinetics were obtained using biochemical radioligand binding assays. Read More

View Article and Full-Text PDF
December 2020

Application of bioluminescence resonance energy transfer-based cell tracking approach in bone tissue engineering.

J Tissue Eng 2021 Jan-Dec;12:2041731421995465. Epub 2021 Feb 16.

Division of Orthodontics, The Ohio State University College of Dentistry, Columbus, OH, USA.

Bioluminescent imaging (BLI) has emerged as a popular in vivo tracking modality in bone regeneration studies stemming from its clear advantages: non-invasive, real-time, and inexpensive. We recently adopted bioluminescence resonance energy transfer (BRET) principle to improve BLI cell tracking and generated the brightest bioluminescent signal known to date, which thus enables more sensitive real-time cell tracking at deep tissue level. In the present study, we brought BRET-based cell tracking strategy into the field of bone tissue engineering for the first time. Read More

View Article and Full-Text PDF
February 2021

Physical activity modulates mononuclear phagocytes in mammary tissue and inhibits tumor growth in mice.

PeerJ 2021 19;9:e10725. Epub 2021 Jan 19.

Norman Cousins Center for PNI, Semel Institute for Neuroscience, University of California, Los Angeles, CA, United States of America.

The risk for breast cancer is significantly reduced in persons who engage in greater amounts of physical activity, and greater physical activity before or after diagnosis associates with reduced disease-specific mortality. Previous mechanistic studies indicate that components of innate immunity can mediate an inhibitory effect of physical activity on several types of tumor. However, in breast cancer specifically, the myeloid compartment of innate immunity is thought to exhibit high propensity for an immunosuppressive role that obstructs anti-tumor immunity. Read More

View Article and Full-Text PDF
January 2021

Evaluation of NanoLuc substrates for bioluminescence imaging of transferred cells in mice.

J Photochem Photobiol B 2021 Mar 26;216:112128. Epub 2021 Jan 26.

Erasmus Medical Center, Optical molecular Imaging, Department of Radiology and Nuclear Medicine, Rotterdam, Netherlands; Erasmus Medical Center, Department of Molecular Genetics, Rotterdam, Netherlands. Electronic address:

NanoLuc luciferase recently gained popularity due to its small size and superior bioluminescence performance. For in vivo imaging applications, NanoLuc has been limited by its substrate furimazine, which has low solubility and bioavailability. Herein, we compared the performances of recently reported NanoLuc luciferase substrates for in vivo imaging in mice. Read More

View Article and Full-Text PDF

Olfactory marker protein interacts with adenosine nucleotide derivatives.

Biochem Biophys Rep 2021 Mar 12;25:100887. Epub 2021 Jan 12.

Department of Physiology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.

Olfactory marker protein (OMP) is a genetic signature for mature olfactory receptor neurons (ORNs). Recently, it has been proposed that OMP directly captures odour-induced cAMP to swiftly terminate the olfactory signal transduction to maintain neuronal sensitivity. In the present study, we show that OMP can also interact with other adenosine nucleotides as ATP, ADP and AMP with different affinities. Read More

View Article and Full-Text PDF

High road mortality during female-biased larval dispersal in an iconic beetle.

Behav Ecol Sociobiol 2021 16;75(1):26. Epub 2021 Jan 16.

Department of Ecology and Genetics, University of Oulu, Post Box 3000, 90014 Oulu, Finland.

Abstract: Animals often disperse from one habitat to another to access mates or suitable breeding sites. The costs and benefits of such movements depend, in part, on the dispersing individuals' phenotypes, including their sex and age. Here we investigated dispersal and road-related mortality in larvae of a bioluminescent beetle, the European common glow-worm, , in relation to habitat, sex and proximity of pupation. Read More

View Article and Full-Text PDF
January 2021

Mycobacteriophage SWU1-Functionalized magnetic particles for facile bioluminescent detection of Mycobacterium smegmatis.

Anal Chim Acta 2021 Feb 8;1145:17-25. Epub 2020 Dec 8.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing, 400716, China. Electronic address:

Mycobacterium tuberculosis (M. tuberculosis), the causative agent of tuberculosis, ranks one of the most dangerous pathogens for its large deaths toll. Due to its characteristic extremely slow growth, the conventional culture-based protocol cannot meet the requirement for the efficient diagnosis of M. Read More

View Article and Full-Text PDF
February 2021

Ratiometric Bioluminescent Indicator for Simple and Rapid Diagnosis of Bilirubin.

ACS Sens 2021 03 14;6(3):889-895. Epub 2021 Jan 14.

Graduate School of Frontier Biosciences, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Japan.

Bilirubin in human blood is highly important as a general index of one's physical condition because its concentration changes under the influence of several diseases. In particular, in newborns, jaundice is one of the most common diseases involving unconjugated bilirubin (UCBR), causing serious symptoms such as nuclear jaundice and deafness. Therefore, a frequent measurement of the UCBR levels in the blood is important. Read More

View Article and Full-Text PDF

Development of a Murine Model of Pyogenic Flexor Tenosynovitis.

J Bone Joint Surg Am 2021 Mar;103(5):432-438

Center for Musculoskeletal Research, Department of Orthopaedics and Rehabilitation, University of Rochester Medical Center, Rochester, New York.

Background: Pyogenic flexor tenosynovitis is a debilitating infection of the hand flexor tendon sheath with high morbidity despite standard treatments of empiric antibiotics with irrigation and debridement. In vivo studies in the available literature have used avian models, but these models are difficult to scale and maintain. The purpose of this study was to demonstrate the plausibility of a murine model of pyogenic flexor tenosynovitis utilizing bioluminescence imaging and tissue analysis at harvest. Read More

View Article and Full-Text PDF

Catalytic polymersomes to produce strong and long-lasting bioluminescence.

Nanoscale 2021 Jan 22;13(1):66-70. Epub 2020 Dec 22.

Department of Chemistry, University of Basel, Mattenstrasse 24a, Basel-4002, Switzerland.

Here, we introduce an artificial bioluminescent nanocompartment based on the encapsulation of light-producing enzymes, luciferases, inside polymersomes. We exploit nanocompartmentalization to enhance luciferase stability in a cellular environment but also to positively modulate enzyme kinetics to achieve a long-lasting glow type signal. These features pave the way for expanding bioluminescence to nanotechnology-based applications. Read More

View Article and Full-Text PDF
January 2021

Improved Aliivibrio fischeri based-toxicity assay: Graphene-oxide as a sensitivity booster with a mobile-phone application.

J Hazard Mater 2021 03 31;406:124434. Epub 2020 Oct 31.

Nanobioelectronics & Biosensors Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, Bellaterra, 08193 Barcelona, Spain; ICREA, Institució Catalana de Recerca i Estudis Avançats, Pg. Lluís Companys 23, 08010 Barcelona, Spain. Electronic address:

Recently, many bioluminescence-based applications have arisen in several fields, such as biosensing, bioimaging, molecular biology, and human health diagnosis. Among all bioluminescent organisms, Aliivibrio fischeri (A. fischeri) is a bioluminescent bacterium used to carry out water toxicity assays since the late 1970s. Read More

View Article and Full-Text PDF

Simultaneous diffuse optical and bioluminescence tomography to account for signal attenuation to improve source localization.

Biomed Opt Express 2020 Nov 16;11(11):6428-6444. Epub 2020 Oct 16.

School of Computer Science, College of Engineering and Physical Sciences, University of Birmingham, UK.

Photonics based pre-clinical imaging is an extensively used technique to allow for the study of biologically relevant activity typically within a small-mouse model. Namely, bioluminescent tomography (BLT) attempts to tomographically reconstruct the 3-dimensional spatial light distribution of luminophores within a small animal given surface light measurements and known underlying optical parameters. Often it is the case where these optical parameters are unknown leading to the use of a 'best' guess approach or to direct measurements using either a multi-modal or dedicated system. Read More

View Article and Full-Text PDF
November 2020

Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated Macrophages.

Cancers (Basel) 2020 Nov 26;12(12). Epub 2020 Nov 26.

Translational Research Center, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.

Visfatin, an adipocytokine highly expressed in breast tumor tissues, is associated with breast cancer progression. Recent studies showed that adipocytokines mediate tumor development through adipocytokine tumor-stromal interactions in the tumor microenvironment. This study focused on the interaction between one key stromal constituent-tumor-associated macrophages-and visfatin. Read More

View Article and Full-Text PDF
November 2020

Solution structures of the Shewanella woodyi H-NOX protein in the presence and absence of soluble guanylyl cyclase stimulator IWP-051.

Protein Sci 2021 02 10;30(2):448-463. Epub 2020 Dec 10.

Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona, USA.

Heme-nitric oxide/oxygen binding (H-NOX) domains bind gaseous ligands for signal transduction in organisms spanning prokaryotic and eukaryotic kingdoms. In the bioluminescent marine bacterium Shewanella woodyi (Sw), H-NOX proteins regulate quorum sensing and biofilm formation. In higher animals, soluble guanylyl cyclase (sGC) binds nitric oxide with an H-NOX domain to induce cyclase activity and regulate vascular tone, wound healing and memory formation. Read More

View Article and Full-Text PDF
February 2021

Tandem reassembly of split luciferase-DNA chimeras for bioluminescent detection of attomolar circulating microRNAs using a smartphone.

Biosens Bioelectron 2021 Feb 18;173:112824. Epub 2020 Nov 18.

College of Life Sciences, South-Central University for Nationalities, Wuhan, 430074, PR China. Electronic address:

Detection of dysregulated circulating microRNAs (miRNAs) in human biofluids is a fundamental ability to determine tumor occurrence and metastasis in a minimally invasive fashion. However, the requirements for sophisticated instruments and professional personnel impede the translation of miRNA tests into routine clinical diagnostics, especially for resource-limited regions. Herein, we developed a DNA-guided bioluminescence strategy for the detection of circulating miRNAs. Read More

View Article and Full-Text PDF
February 2021

The size of signal detection and emission organs in a synchronous firefly: sexual dimorphism, allometry and assortative mating.

PeerJ 2020 27;8:e10127. Epub 2020 Oct 27.

Departamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Mexico City, México.

The size of the organs responsible for emitting and detecting sexual communication signals is a likely target for selection. Communication via bioluminescent signals in synchronous fireflies is a promising model to test hypotheses regarding differences between males and females in the effect of the size of signal emission and detection organs on fitness components. Synchronous firefly species congregate in large numbers during the mating season, displaying bioluminescent signals aimed at potential mates during relatively short nightly periods. Read More

View Article and Full-Text PDF
October 2020

A Bioluminescent Probe for Simultaneously Imaging Esterase and Histone Deacetylase Activity in a Tumor.

Anal Chem 2020 12 10;92(23):15275-15279. Epub 2020 Nov 10.

Hefei National Laboratory of Physical Sciences at Microscale, Department of Chemistry, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, China.

The monitoring of esterase (CES) and histone deacetylase (HDAC) activity in living cells has great potential for rapid diagnosis of malignant tumors. At present, using one bioluminescence (BL) probe to simultaneously detect (or image) these two enzymes' activity in tumors has not been reported. Herein, a bioluminescence "turn-on" probe (6-acetamidohexanoic acid-d-luciferin) was rationally designed for simultaneously imaging CES and HDAC activity with excellent sensitivity and selectivity. Read More

View Article and Full-Text PDF
December 2020

Retraction of "Novel Bioluminescent Activatable Reporter for Src Tyrosine Kinase Activity in Living Mice".

Theranostics 2020 30;10(25):11862. Epub 2020 Sep 30.

Department of Medical Oncology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.

[This retracts the article DOI: 10.7150/thno.14306. Read More

View Article and Full-Text PDF
September 2020

Selection, drift, and constraint in cypridinid luciferases and the diversification of bioluminescent signals in sea fireflies.

Mol Ecol 2021 04 5;30(8):1864-1879. Epub 2020 Nov 5.

Department of Ecology, Evolution, & Marine Biology, University of California, Santa Barbara, Santa Barbara, CA, USA.

Understanding the genetic causes of evolutionary diversification is challenging because differences across species are complex, often involving many genes. However, cases where single or few genetic loci affect a trait that varies dramatically across a radiation of species provide tractable opportunities to understand the genetics of diversification. Here, we begin to explore how diversification of bioluminescent signals across species of cypridinid ostracods ("sea fireflies") was influenced by evolution of a single gene, cypridinid-luciferase. Read More

View Article and Full-Text PDF