29 results match your criteria biology zavareh

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Effect of Mouse Ovarian Vitrification on Promoter Methylation of Inhba and Inhbb in Granulosa Cells of Follicles.

Cryo Letters 2021 Mar-Apr;42(2):67-72

School of Biology, Damghan University, Damghan; Institute of Biological Sciences, Damghan University, Damghan, Iran.

Background: Cryopreservation can induce cellular, genomic, and epigenetic abnormalities.

Objective: To analyse the impact of ovarian vitrification on follicular development and its epigenetic effect on promoter methylation of Inhba and Inhbb in granulosa cells.

Materials And Methods: Mouse ovaries were divided into control, toxicity, and vitrified groups. Read More

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Epigenetic effects of Bisphenol A on granulosa cells of mouse follicles during in vitro culture: An experimental study.

Int J Reprod Biomed 2021 Feb 21;19(2):129-136. Epub 2021 Feb 21.

School of Biology, Damghan University, Damghan, Iran.

Background: Bisphenol A (BPA), a synthetic endocrine-disrupting chemical, is a reproductive toxicant. Granulosa cells have significant roles in follicle development, and KIT ligand (KITL) and Anti-Müllerian hormone (AMH) are essential biomolecules produced by them during folliculogenesis.

Objective: Due to the widespread use of BPA and its potential epigenetic effects, this study examined the impact of BPA on promoter methylation of and genes in mouse granulosa cells. Read More

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February 2021

The effect of sodium selenite on apoptotic gene expression and development of cultured mouse oocytes in comparison with obtained oocytes.

Vet Res Forum 2020 15;11(4):377-383. Epub 2020 Dec 15.

Prevention of Metabolic Research Disorder Center, Research Institute for Endocrine Disorder, Shahid Beheshti University of Medical Science, Tehran, Iran.

maturation (IVM) of oocytes is widely used in assisted reproduction technologies. The present study aimed to improve the oocyte maturation and its development through enriching the culture media with sodium selenite (SS). Moreover, the effects of SS on the expression of the oocytes apoptosis-related genes were assessed. Read More

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December 2020

Making HSP90 Inhibitors Great Again? Unite for Better Cancer Immunotherapy.

Authors:
Michael W Graner

Cell Chem Biol 2021 Feb;28(2):118-120

Department of Neurosurgery, University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA. Electronic address:

HSP90 inhibitors are in numerous cancer clinical trials, but treatments often induce toxicity at effective dosages. In this issue of Cell Chemical Biology, Zavareh et al. (2020) serendipitously found that HSP90 inhibitors, at manageable doses, can reduce target cell expression of immune checkpoint molecules, potentially enabling improved anti-cancer immunotherapy. Read More

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February 2021

Assessment of DNA Repair Gene Expressions in Vitrified Mouse Preantral Follicles.

Cell J 2020 Jul 18;22(Suppl 1):81-88. Epub 2020 Jul 18.

School of Biology, Damghan University, Damghan, Iran.

Objective: Vitrification of the ovarian tissue is one of the techniques recommended for preserving the fertility of women who are dealing with infertility. Despite its benefits, our information about the molecular aspects of ovarian follicles vitrification is somehow ambiguous. Therefore, the aim of this study was to evaluate the expression pattern of DNA repair genes in vitrified preantral follicles. Read More

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Exposure to cell phone induce oxidative stress in mice preantral follicles during in vitro cultivation: An experimental study.

Int J Reprod Biomed 2019 Sep 22;17(9):637-646. Epub 2019 Sep 22.

School of Biology Damghan University Damghan Iran.

Background: Radiations emitting from mobile phones have been proposed to affect people's health, mediated by various mechanisms like induction of oxidative stress.

Objective: This study aims to investigate the effect of cell phone exposure on the oxidative status of mice preantral follicles (PFs) during in vitro culture.

Materials And Methods: PFs (n░=░2580) were isolated mechanically from 16 to 18 day-old NMRI mice (n░=░50) and divided into control and cell phone-exposed groups. Read More

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September 2019

The Effect of Radiation Emitted by Cell Phone on The Gelatinolytic Activity of Matrix Metalloproteinase-2 and -9 of Mouse Pre-Antral Follicles during In Vitro Culture.

Cell J 2020 Apr 8;22(1):1-8. Epub 2019 Sep 8.

School of Biology, Damghan University, Damghan, Iran.

Objective: The unfavorable effects of electromagnetic radiation (EMR) emitted by the cell phone on reproduction health are controversial. Metalloproteinases play a vital role in ovarian follicle development. This study was designed to investigate the effects of exposure to the cell phone on the gelatinolytic activity of cultured mouse pre-antral follicle. Read More

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Implications from early life stress on the development of mouse ovarian follicles: Focus on oxidative stress.

J Obstet Gynaecol Res 2019 Aug 17;45(8):1506-1514. Epub 2019 Jun 17.

School of Biology, Damghan University, Damghan, Iran.

Aim: The early life stress has significant long-term effects on the development of the offspring. This study was undertaken to verify if maternal separation as a stressor agent affects the oxidative status and developmental competence of mouse pre-antral follicles (PF) during in vitro culture period.

Methods: Female litters of National Medical Research Institute mice were divided into two groups: maternally separated group (MS), separated from the mothers for 6 h per day from postnatal days 2-16; and the rest considered as the control group, which left undisturbed over the 14 days. Read More

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Assessment of novel core-shell FeO@poly l‑DOPA nanoparticles for targeted Taxol® delivery to breast tumor in a mouse model.

Mater Sci Eng C Mater Biol Appl 2018 Dec 4;93:1036-1043. Epub 2018 Sep 4.

Department of Biohemistry, Damghan Branch, Islamic Azad University, Damghan, Iran.

Drug delivery systems using nanoparticles can deliver to tumor cells without affecting normal cells. In this study, a novel well dispersed magnetic nano drug was synthesized. Thus, a selective drug delivery system was designed for potential cancer treatment. Read More

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December 2018

Discovery and optimization of aspartate aminotransferase 1 inhibitors to target redox balance in pancreatic ductal adenocarcinoma.

Bioorg Med Chem Lett 2018 09 27;28(16):2675-2678. Epub 2018 Apr 27.

California Institute for Biomedical Research, 11119 North Torrey Pines Road, La Jolla, CA 92037, USA; Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. Electronic address:

Pancreatic ductal adenocarcinoma (PDAC) is a lethal malignancy that is extremely refractory to the therapeutic approaches that have been evaluated to date. Recently, it has been demonstrated that PDAC tumors are dependent upon a metabolic pathway involving aspartate aminotransferase 1, also known as glutamate-oxaloacetate transaminase 1 (GOT1), for the maintenance of redox homeostasis and sustained proliferation. As such, small molecule inhibitors targeting this metabolic pathway may provide a novel therapeutic approach for the treatment of this devastating disease. Read More

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September 2018

Evaluation of changes in the expression of Wnt/β-catenin target genes in mouse reproductive tissues during estrous cycle: An experimental study.

Int J Reprod Biomed 2018 Feb;16(2):69-76

School of Biology, Damghan University, Damghan, Iran.

Background: The Wingless-type (Wnt)/β-catenin signaling pathway controls cell homeostasis. Reproductive tissues are dynamic in response to steroidal hormone changes. Steroidal hormones are known to control the Wnt/β-catenin pathway, but their role in reproductive tissues remains unknown. Read More

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February 2018

Changes in the expression of OCT4 in mouse ovary during estrous cycle.

Vet Res Forum 2017 15;8(1):43-48. Epub 2017 Mar 15.

Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

The transcriptional factor OCT4 regulates pluripotency of stem cells and has an important role during oocyte growth. Whereas, its role has remained ambiguous in ovarian tissue during reproductive cycle. Therefore, this study was aimed to investigate the expression patterns of OCT4 in mouse ovaries during the normal estrous cycle. Read More

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Antioxidant properties of coenzyme Q10-pretreated mouse pre-antral follicles derived from vitrified ovaries.

J Obstet Gynaecol Res 2017 Jan 9;43(1):140-148. Epub 2016 Dec 9.

School of Biology, Damghan University, Damghan, Iran.

Aim: This study evaluated the antioxidant status of pre-antral follicles derived from vitrified ovaries pretreated with coenzyme Q10 (CoQ10).

Methods: Mouse pre-antral follicles derived from fresh and vitrified warmed ovarian tissue were cultured with or without CoQ10 (50 μmol/L). Follicular growth, total antioxidant capacity (TAC), malondialdehyde (MDA) level, and superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) activity during cultivation were assessed. Read More

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January 2017

The Biology of β-D-mannuronic acid (M2000) on Human Dendritic Cell Based on MicroRNA-155 and MicroRNA-221.

Curr Drug Discov Technol 2017 ;14(1):53-58

Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Box: 14155-6446, Tehran, Iran.

Background: The aim of this study was to evaluate the effect of ß-Dmannuronic acid (M2000) on related miRNAs to dendritic cells (DCs) differentiation. DC-based immunosuppressive drugs can suppress the progression of autoimmune diseases, however, their notable side effects in increasing the risk of infectious diseases and cancers should be considered. The β-D-mannuronic acid, as a novel non-steroidal anti-inflammatory agent, has been tested in various experimental models. Read More

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Expression of pluripotent stem cell markers in mouse uterine tissue during estrous cycle.

Vet Res Forum 2016 15;7(3):181-188. Epub 2016 Sep 15.

Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran; ; Institute of Biological Sciences, Damghan University, Damghan, Iran.

It was assumed that uterine stem cells are responsible for the unique regenerative capacity of uterine. Therefore, the aim of the present study was to investigate the expression of the pluripotent stem cell markers in the mice uterine tissue during different stages of estrous cycles. Twelve virgin female NMRI mice (6 to 8 weeks old) were considered at proestrus, estrus, metestrus and diestrus according to the cell types observed in the vaginal smear and underwent hysterectomy operation. Read More

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September 2016

Synthesis of polydopamine as a new and biocompatible coating of magnetic nanoparticles for delivery of doxorubicin in mouse breast adenocarcinoma.

Cancer Chemother Pharmacol 2016 Nov 17;78(5):1073-1084. Epub 2016 Oct 17.

Department of Chemistry, Damghan Branch, Islamic Azad University, Damghan, Iran.

Purpose: Carrier-mediated drug delivery systems can be used to increase the intracellular concentration of drugs in cancerous cells, thereby improving drug biodistribution and minimizing unwanted side effects. This study aimed to investigate the effect of synthesized magnetic molecularly imprinted polydopamine for controlled doxorubicin (DOX) delivery in a breast adenocarcinoma model of BALB/c mice with an external magnetic field.

Methods: The synthesized DOX-imprinted polydopamine (DOX-IP) was characterized using Fourier transform infrared spectroscopy and scanning electron microscopy. Read More

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November 2016

Augmenting effect of vitrification on lipid peroxidation in mouse preantral follicle during cultivation: Modulation by coenzyme Q.

Syst Biol Reprod Med 2016 Dec 3;62(6):404-414. Epub 2016 Oct 3.

a School of Biology, Damghan University , Damghan , Iran.

Cryopreservation-induced oxidative stress (OS) may lead to lipid peroxidation, which may be responsible for decreased cell survival rate. Coenzyme Q10 (CoQ10) as a potent antioxidant may improve cell viability by neutralizing OS. In this study, oxidative lipid injury following the vitrification of preantral follicles was investigated. Read More

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December 2016

The effect of steroid hormones on the mRNA expression of oct4 and sox2 in uterine tissue of the ovariectomized mice model of menopause.

Int J Reprod Biomed 2016 Jul;14(7):471-6

School of Biology, Damghan University, Damghan, Iran.; Institute of Biological Sciences, Damghan University, Damghan, Iran.

Background: The uterus is a dynamic tissue responding to hormonal changes during reproductive cycles. As such, uterine stem cells have been studied in recent years. Transcription factors oct4 and sox2 are critical for effective maintenance of pluripotent cell identity. Read More

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Vitrification affects the expression of matrix metalloproteinases and their tissue inhibitors of mouse ovarian tissue.

Int J Reprod Biomed 2016 Mar;14(3):173-80

School of Biology, Damghan University, Damghan, Iran.; Institute of Biological Sciences, Damghan University, Damghan, Iran.

Background: One of the most major obstacles of ovarian tissue vitrification is suboptimal developmental competence of follicles. Matrix metalloproteinases 2 (MMP-2) and 9 (MMP-9) and their tissue inhibitors TIMP-1 and TIMP-2 are involved in the remodeling of the extracellular matrix in the ovaries.

Objective: This study aimed to evaluate the expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 genes in the preantral follicles derived from vitrified mouse ovaries. Read More

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Effect of In Vitro Maturation Technique and Alpha Lipoic Acid Supplementation on Oocyte Maturation Rate: Focus on Oxidative Status of Oocytes.

Int J Fertil Steril 2016 Jan-Mar;9(4):442-51. Epub 2015 Dec 23.

School of Biology, Damghan University, Damghan, Iran.

Background: Therapeutic potential of in vitro maturation (IVM) in infertility is growing with great promise. Although significant progress is obtained in recent years, existing IVM protocols are far from favorable results. The first aim of this study was to investigate whether two step IVM manner change reactive oxygen species (ROS) and total anti- oxidant capacity (TAC) levels. Read More

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Evaluation of magnetic nanoparticles coated by 5-fluorouracil imprinted polymer for controlled drug delivery in mouse breast cancer model.

Int J Pharm 2016 Jan 2;497(1-2):228-38. Epub 2015 Dec 2.

School of Biology, Damghan University, Damghan, Iran.

Nanoparticles (NPs) have been extensively investigated to improve delivery efficiency of therapeutic and diagnostic agents. In this study, magnetic molecularly imprinted polymer (MIP) was synthesized by using polydopamine. Synthesized MIP was used for controlled 5-fluorouracil (5-FU) delivery in a spontaneous model of breast adenocarcinoma in Balb/c mice in the presence of an external magnetic field. Read More

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January 2016

Comparison of oxidative status of mouse pre-antral follicles derived from vitrified whole ovarian tissue and vitrified pre-antral follicles in the presence of alpha lipoic acid.

J Obstet Gynaecol Res 2014 Jun;40(6):1680-8

School of Biology, Damghan University, Damghan, Islamic Republic of Iran.

Aim: The main goal of this study was to compare developmental competence and oxidative status of vitrified-warmed pre-antral follicles (VPF) with pre-antral follicles derived from vitrified-warmed ovarian tissue (VOF) in the presence of alpha lipoic acid (ALA).

Materials And Methods: Ovarian tissue and isolated pre-antral follicles were exposed to equilibration solution and then vitrification solution. After thawing of LN2 snap-frozen samples, pre-antral follicles were cultured with or without ALA for 12 days that followed by hCG-induced ovulation. Read More

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Total oxidative status of mouse vitrified pre-antral follicles with pre-treatment of alpha lipoic acid.

Iran Biomed J 2014 07;18(3):181-8

Laboratory of Molecular and Cellular Biology, Department of Basic Veterinary Sciences, School of Veterinary Medicine, Razi University, Kermanshah, Iran.

Background: Cryopreservation of pre-antral follicles is a hopeful technique to preserve female fertility. The aim of the present study was to evaluate reactive oxygen species (ROS) and total antioxidant capacity (TAC) levels of mouse vitrified pre-antral follicles in the presence of alpha lipoic acid (ALA).

Methods: Isolated pre-antral follicles (140-150 µm in diameter) were divided into vitrified-warmed and fresh groups. Read More

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The impact of alpha lipoic acid on developmental competence of mouse vitrified pre-antral follicles in comparison to those isolated from vitrified ovaries.

Iran J Reprod Med 2014 Jan;12(1):57-64

Laboratory of Molecular and Cellular Biology, Department of Basic Veterinary Sciences, School of Veterinary Medicine, Razi University, Kermanshah, Iran.

Background: Cryopreservation of ovarian tissues and pre-antral follicles is a promising prospect for preservation of women fertility.

Objective: The aim of this study was to evaluate the in vitro developmental competence of mouse vitrified pre-antral follicles in comparison to isolated pre-antral follicles derived from vitrified ovaries in the presence of alpha lipoic acid (ALA).

Materials And Methods: Pre-antral follicles derived from fresh, vitrified-warmed ovarian tissues and vitrified-warmed pre-antral follicles were cultured individually with or without ALA, followed by adding hCG to induce ovulation. Read More

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January 2014

The Effects of cAMP-elevating Agents and Alpha Lipoic Acid on In Vitro Maturation of Mouse Germinal Vesicle Oocytes.

J Reprod Infertil 2013 Oct;14(4):173-83

Laboratory of Molecular and Cellular Biology, Department of Basic Veterinary Sciences, School of Veterinary Medicine, Razi University, Kermanshah, Iran.

Background: In spite of extensive efforts to improve in vitro oocyte maturation, the obtained results are not very efficient. This study was conducted to assess impacts of cAMP elevating agents and alpha lipoic acid (ALA) on in vitro oocyte maturation and fertilization.

Methods: Mouse germinal vesicle (GV) oocytes were categorized into cumulus denuded oocytes (DOs; n=896) and cumulus oocyte complexes (COCs; n=1077) groups. Read More

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October 2013

The effects of progesterone on oocyte maturation and embryo development.

Int J Fertil Steril 2013 Jul 31;7(2):74-81. Epub 2013 Jul 31.

Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran.

Oocyte maturation and embryo development are controlled by intra-ovarian factors such as steroid hormones. Progesterone (P4) exists in the follicular fluid that contributes to normal mammalian ovarian function and has several critical functions during embryo development and implantation, including endometrial receptivity, embryonic survival during gestation and transformation of the endometrial stromal cells to decidual cells. It is well known that the physiological effects of P4 during the pre-implantation stages of some mammal's embryos are mediated by P4 receptors and their gene expression is determined. Read More

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Combined Treatment of Androgen-Independent Prostate Cancer Cell Line DU145 with Chemotherapeutic Agents and Lithium Chloride: Effect on Growth Arrest and/or Apoptosis.

Avicenna J Med Biotechnol 2012 Apr;4(2):75-87

School of Biology, University College of Science, University of Tehran, Tehran, Iran.

Hormone-independent prostate cancer cell lines are resistant to antineoplastic drugs, this study sought to determine the usefulness of lithium chloride as an inhibitor of glycogen synthase kinase-3β to increase the cytotoxic effect of doxorubicin, etoposide or vinblastine antineoplastic drugs on DU145 cells. Combination effect was assessed by using low and IC(50) doses of drugs + lithium chloride. Subsequently, cell cycle analysis and p53 levels and its subcellular localization as a key regulator of cell cycle were assessed. Read More

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Complex network analysis in microbial systems: theory and examples.

Methods Mol Biol 2012 ;881:551-71

Department of Biotechnology, Norwegian University of Science and Technology, NTNU, Trondheim, Norway.

An essential idea in the area of Systems Biology is that a good understanding of interactions between components is crucial for developing deep knowledge of the functioning of the system as a whole. Network analysis is an approach uniquely suited to uncover patterns and organizing principles in a wide variety of complex systems. In this chapter, we will give a detailed description of central network concepts and their algorithmic implementation, and demonstrate how they may be applied on two biological networks: the protein-interaction network of Mus musculus and the reconstructed genome-scale metabolic network of the bacterium Yersinia pestis. Read More

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September 2012

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles.

J Assist Reprod Genet 2012 Feb 10;29(2):175-83. Epub 2012 Jan 10.

School of Biology, Damghan University, Damghan, Iran.

Purpose: This study was designed to investigate the effect of alpha-lipoic acid (ALA) on reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and developmental competence of cultured pre-antral follicles derived from mouse ovarian tissue.

Methods: Pre-antral follicles were isolated from immature mouse ovaries and were cultured in α- minimal essential medium supplemented with different concentrations (0, 50, 100, 250 and 500 uM) of ALA. Follicular growth, oocyte maturation and embryo development were evaluated. Read More

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February 2012
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