678 results match your criteria biofilm-related infections

Extracellular DNA released by glycine-auxotrophic Staphylococcus epidermidis small colony variant facilitates catheter-related infections.

Commun Biol 2021 Jul 22;4(1):904. Epub 2021 Jul 22.

Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.

Though a definitive link between small colony variants (SCVs) and implant-related staphylococcal infections has been well-established, the specific underlying mechanism remains an ill-explored field. The present study analyzes the role SCVs play in catheter infection by performing genomic and metabolic analyses, as well as analyzing biofilm formation and impacts of glycine on growth and peptidoglycan-linking rate, on a clinically typical Staphylococcus epidermidis case harboring stable SCV, normal counterpart (NC) and nonstable SCV. Our findings reveal that S. Read More

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Alternatives to Conventional Antibiotic Therapy: Potential Therapeutic Strategies of Combating Antimicrobial-Resistance and Biofilm-Related Infections.

Mol Biotechnol 2021 Jul 26. Epub 2021 Jul 26.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610065, Sichuan, People's Republic of China.

Antibiotics have been denoted as the orthodox therapeutic agents for fighting bacteria-related infections in clinical practices for decades. Nevertheless, overuse of antibiotics has led to the upsurge of species with antimicrobial resistance (AMR) or multi-drug resistance. Bacteria can also grow into the biofilm, which accounts for at least two-thirds of infections. Read More

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Outcomes of patients with unexpected diagnosis of infection at total hip or total knee arthroplasty revisions.

Int Orthop 2021 Jul 22. Epub 2021 Jul 22.

IRCCS Humanitas Research Hospital, Via Manzoni 56, Rozzano, 20089, Milan, Italy.

Purpose: The pre-operative differential diagnosis between periprosthetic joint infections (PJIs) and aseptic failure is challenging particularly in low virulence and biofilm-related infections. This study aimed to assess the incidence and survival of patients with unexpected PJIs in a presumed aseptic revision of total hip (THA) and knee (TKA) arthroplasties.

Methods: A retrospective analysis of a prospective cohort of patients was performed with 295 patients undergoing THA (n = 241) or TKA (n = 54) revision for presumed aseptic causes. Read More

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rpoS-mutation variants are selected in Pseudomonas aeruginosa biofilms under imipenem pressure.

Cell Biosci 2021 Jul 21;11(1):138. Epub 2021 Jul 21.

School of Medicine, Southern University of Science and Technology, Shenzhen, 518055, Guangdong, People's Republic of China.

Background: Pseudomonas aeruginosa is a notorious opportunistic pathogen causing various types of biofilm-related infections. Biofilm formation is a unique microbial strategy that allows P. aeruginosa to survive adverse conditions such as antibiotic treatment and human immune clearance. Read More

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Antibacterial, Antibiofilm, and Antioxidant Activity of Polysaccharides Obtained from Fresh Sarcotesta of : Bioactive Polysaccharide that Can Be Exploited as a Novel Biocontrol Agent.

Evid Based Complement Alternat Med 2021 15;2021:5518403. Epub 2021 Jun 15.

Institute of Animal Husbandry and Veterinary Medicine, Jinzhou Medical University, Jinzhou 121001, Liaoning, China.

() biofilm plays an important role in the persistence of chronic infection due to its resistance to antibiotics. Because of their functional diversity, active polysaccharide is increasingly being applied as a biocontrol agent to inhibit the formation of biofilm by pathogens. In this study, a new polysaccharide, GBSPII-1, isolated from the fresh sarcotesta of . Read More

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Anti-Biofilm Molecules Targeting Functional Amyloids.

Antibiotics (Basel) 2021 Jun 29;10(7). Epub 2021 Jun 29.

Instituto de Agrobiotecnología (Idab), CSIC-Gobierno de Navarra, 31192 Navarra, Spain.

The choice of an effective therapeutic strategy in the treatment of biofilm-related infections is a significant issue. Amyloids, which have been historically related to human diseases, are now considered to be prevailing structural components of the biofilm matrix in a wide range of bacteria. This assumption creates the potential for an exciting research area, in which functional amyloids are considered to be attractive targets for drug development to dissemble biofilm structures. Read More

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Effect of the Combination of Levofloxacin with Cationic Carbosilane Dendron and Peptide in the Prevention and Treatment of Biofilms.

Polymers (Basel) 2021 Jun 29;13(13). Epub 2021 Jun 29.

Department of Organic and Inorganic Chemistry, and Research Institute in Chemistry "Andrés M. del Río" (IQAR), Universidad de Alcala, 28805 Madrid, Spain.

Antibiotic resistance and biofilm-related infections, persistent in conventional antimicrobial treatment, are continuously increasing and represent a major health problem worldwide. Therefore, the development of new effective treatments to prevent and treat biofilm-related infections represents a crucial challenge. Unfortunately, the extensive use of antibiotics has led to an increase of resistant bacteria with the subsequent loss of effectivity of commercial antibiotics, mainly due to antibiotic resistance and the ability of some bacteria to form microbial communities in biotic or abiotic surfaces (biofilms). Read More

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Strategies for Interfering With Bacterial Early Stage Biofilms.

Front Microbiol 2021 8;12:675843. Epub 2021 Jun 8.

Innovative Engineering Research Center of Veterinary Pharmaceutics, Department of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.

Biofilm-related bacteria show high resistance to antimicrobial treatments, posing a remarkable challenge to human health. Given bacterial dormancy and high expression of efflux pumps, persistent infections caused by mature biofilms are not easy to treat, thereby driving researchers toward the discovery of many anti-biofilm molecules that can intervene in early stage biofilms formation to inhibit further development and maturity. Compared with mature biofilms, early stage biofilms have fragile structures, vigorous metabolisms, and early attached bacteria are higher susceptibility to antimicrobials. Read More

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Inhibition of polymicrobial biofilm formation by saw palmetto oil, lauric acid and myristic acid.

Microb Biotechnol 2021 Jun 22. Epub 2021 Jun 22.

School of Chemical Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan, Korea.

Biofilms are communities of bacteria, fungi or yeasts that form on diverse biotic or abiotic surfaces, and play important roles in pathogenesis and drug resistance. A generic saw palmetto oil inhibited biofilm formation by Staphylococcus aureus, Escherichia coli O157:H7 and fungal Candida albicans without affecting their planktonic cell growth. Two main components of the oil, lauric acid and myristic acid, are responsible for this antibiofilm activity. Read More

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Antiplanktonic and antibiofilm effects of two synthetic antimicrobial peptides against .

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 May;46(5):481-487

Hunan Normal University School of Medicine, Changsha 410013.

Objectives: is one of the most common Gram-positive cocci in nosocomial infection, which could adhere to the surface of medical apparatus and causes biofilm-related infections. In the present study, we aim to explore the antimicrobial effects of GH12 and SAAP-148 against

Methods: Micro-dilution methods were used to detect the minimal inhibitory/bactericidal concentration of peptides on . Biofilm formation positive type strain was used to determine the antibiofilm effects of the peptides. Read More

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Enhanced Eradication of Mucin-Embedded Bacterial Biofilm by Locally Delivered Antibiotics in Functionalized Microcontainers.

Macromol Biosci 2021 Jun 12:e2100150. Epub 2021 Jun 12.

The Danish National Research Foundation and Villum Foundation's Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics (IDUN), Department of Health Technology, Technical University of Denmark, Ørsteds Plads 345C, Lyngby, 2800 Kgs., Denmark.

Bacterial biofilm-related infections are difficult to eradicate and require repeated treatments with high doses of antibiotics. Thus, there is an urgent need for new treatment strategies that minimize the use of antibiotics while enhancing biofilm eradication. Functionalized reservoir-based microdevices, such as, microcontainers (MCs), offer, high drug loading capacity, mucus embedment, and tuneable drug release. Read More

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Antibiofilm Activity of Phorbaketals from the Marine Sponge sp. against .

Mar Drugs 2021 May 24;19(6). Epub 2021 May 24.

School of Chemical Engineering, Yeungnam University, Gyeongsan-si 38541, Gyeongsangbukdo, Korea.

Biofilm formation by plays a critical role in the persistence of chronic infections due to its tolerance against antimicrobial agents. Here, we investigated the antibiofilm efficacy of six phorbaketals: phorbaketal A (), phorbaketal A acetate (), phorbaketal B (), phorbaketal B acetate (), phorbaketal C (), and phorbaketal C acetate (), isolated from the Korean marine sponge sp. Of these six compounds, and were found to be effective inhibitors of biofilm formation by two strains, which included a methicillin-resistant . Read More

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Activation of Deoxyribonuclease I by Nicotinamide as a New Strategy to Attenuate Tetracycline-Resistant Biofilms of .

Pharmaceutics 2021 May 31;13(6). Epub 2021 May 31.

Department of Dermatology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.

Biofilms of () (formerly ) are responsible for the persistence and antibiotic resistance of acne vulgaris. In addition to the standard treatments for acne vulgaris, a common adjunctive treatment is the topical administration of nicotinamide (NAM). However, the effects of NAM on biofilms of have never been explored. Read More

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An Antisense RNA Modulates Biofilm Organization of Methicillin-Resistant and Pathogenicity in a Rat Model of Osteomyelitis.

Antibiotics (Basel) 2021 May 19;10(5). Epub 2021 May 19.

Department of Orthopedics, West China Hospital, Sichuan University, Chengdu 610041, China.

() is one of most common opportunistic pathogens and is attributed to several human infections. The increasing incidence of methicillin-resistant (MRSA) is a serious clinical threat for osteomyelitis crisis. The YycFG two-component system of regulates genes associated with biofilm formation. Read More

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Inverse correlation between biofilm production efficiency and antimicrobial resistance in clinical isolates of Pseudomonas aeruginosa.

Microb Pathog 2021 Aug 25;157:104989. Epub 2021 May 25.

Department of Microbiology, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia. Electronic address:

This study assessed the correlation between biofilm formation in Pseudomonas aeruginosa strains with both the level of antibiotic resistance, and the number of virulence- and biofilm-related genes encoded. A total of sixty-six, non-replicate and prospectively collected P. aeruginosa strains were identified and tested. Read More

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Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii.

Sci Rep 2021 May 24;11(1):10751. Epub 2021 May 24.

Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.

We aimed to isolate Acinetobacter baumannii (A. baumannii) from wound infections, determine their resistance and virulence profile, and assess the impact of Silver nanoparticles (AgNPs) on the bacterial growth, virulence and biofilm-related gene expression. AgNPs were synthesized and characterized using TEM, XRD and FTIR spectroscopy. Read More

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A Novel Biofilm-Disrupting Wound Care Technology for the Prevention of Surgical Site Infections Following Total Joint Arthroplasty: A Conceptual Review.

Surg Technol Int 2021 05 18;38. Epub 2021 May 18.

Northwell Health Orthopaedics, Lenox Hill Hospital, New York, New York.

Surgical site infections (SSIs) are a major driver for increased costs following lower extremity joint arthroplasty procedures. It has been estimated that these account for over $2 billion in annual costs in the United States. While many of the current strategies for the prevention and treatment of SSIs target planktonic bacteria, 80 to 90% of bacterial pathogens exist in a sessile state. Read More

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Non-thermal plasma-induced DMPO-OH yields hydrogen peroxide.

Arch Biochem Biophys 2021 Jul 6;705:108901. Epub 2021 May 6.

Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Showa-Ku, Nagoya, 466-8550, Japan; Center for Low-temperature Plasma Sciences, Nagoya University, Chikusa-ku, Nagoya, 464-8603, Japan. Electronic address:

Recent developments in electronics have enabled the medical applications of non-thermal plasma (NTP), which elicits reactive oxygen species (ROS) and reactive nitrogen species (RNS), such as hydroxyl radical (OH), hydrogen peroxide (HO), singlet oxygen (O), superoxide (O), ozone, and nitric oxide at near-physiological temperatures. In preclinical studies or human clinical trials, NTP promotes blood coagulation, eradication of bacterial, viral and biofilm-related infections, wound healing, and cancer cell death. To elucidate the solution-phase biological effects of NTP in the presence of biocompatible reducing agents, we employed electron paramagnetic resonance (EPR) spectroscopy to quantify OH using a spin-trapping probe, 5,5-dimethyl-1-pyrroline-N-oxide (DMPO); O using a fluorescent probe; and O and HO using luminescent probes in the presence of thiols or tempol. Read More

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In Vitro Antimicrobial Susceptibility Testing of Biofilm-Growing Bacteria: Current and Emerging Methods.

Adv Exp Med Biol 2021 May 8. Epub 2021 May 8.

Department of Medical, Oral and Biotechnological Sciences, and Center for Advanced Studies and Technology (CAST), "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy.

The antibiotic susceptibility of bacterial pathogens is typically determined based on planktonic cells, as recommended by several international guidelines. However, most of chronic infections - such as those established in wounds, cystic fibrosis lung, and onto indwelling devices - are associated to the formation of biofilms, communities of clustered bacteria attached onto a surface, abiotic or biotic, and embedded in an extracellular matrix produced by the bacteria and complexed with molecules from the host. Sessile microorganisms show significantly increased tolerance/resistance to antibiotics compared with planktonic counterparts. Read More

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Phenotypic and genotypic characteristics of Acinetobacter baumannii enrolled in the relationship among antibiotic resistance, biofilm formation and motility.

Microb Pathog 2021 Jun 28;155:104922. Epub 2021 Apr 28.

Department of Microbiology and Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, China. Electronic address:

Acinetobacter baumannii is an important pathogen in clinical. The factors of biofilm formation, antibiotic resistance and motility contribute great to A. baumannii in persisting in stressed environment, and further leads to nosocomial infections. Read More

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Combining Visible Light and Non-Focused Ultrasound Significantly Reduces Biofilm While Having Limited Effect on Host Cells.

Microorganisms 2021 Apr 26;9(5). Epub 2021 Apr 26.

Photosonix Medical, Inc., Fort Washington, PA 19034, USA.

Bacterial biofilms are highly resistant to antibiotics and have been implicated in the etiology of 60%-80% of chronic microbial infections. We tested a novel combination of low intensity ultrasound and blue light against biofilm and planktonic bacteria. A laboratory prototype was built which produced both energies uniformly and coincidently from a single treatment head, impinging upon a 4. Read More

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Activity of Liquid and Volatile Fractions of Essential Oils against Biofilm Formed by Selected Reference Strains on Polystyrene and Hydroxyapatite Surfaces.

Pathogens 2021 Apr 23;10(5). Epub 2021 Apr 23.

Department of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy, Medical University of Silesian Piasts in Wroclaw, 50-367 Wrocław, Poland.

Biofilms are surface-attached, structured microbial communities displaying higher tolerance to antimicrobial agents in comparison to planktonic cells. An estimated 80% of all infections are thought to be biofilm-related. The drying pipeline of new antibiotics efficient against biofilm-forming pathogens urges the search for alternative routes of treatment. Read More

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Novel Strategies to Combat Bacterial Biofilms.

Mol Biotechnol 2021 Jul 29;63(7):569-586. Epub 2021 Apr 29.

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Biofilms are considered as a severe problem in the treatment of bacterial infections; their development causes some noticeable resistance to antibacterial agents. Biofilms are responsible for at least two-thirds of all infections, displaying promoted resistance to classical antibiotic treatments. Therefore, finding new alternative therapeutic approaches is essential for the treatment and inhibition of biofilm-related infections. Read More

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A Facile High-Throughput Model of Surface-Independent Staphylococcus aureus Biofilms by Spontaneous Aggregation.

mSphere 2021 04 28;6(2). Epub 2021 Apr 28.

Department of Chemical and Materials Engineering, San José State University, San José, California, USA

Many microbes in their natural habitats are found in biofilm ecosystems attached to surfaces and not as free-floating (planktonic) organisms. Furthermore, it is estimated that nearly 80% of human infections are associated with biofilms. Biofilms are traditionally defined as three-dimensional, structured microbial communities that are attached to a surface and encased in a matrix of exopolymeric material. Read More

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[Effect of N-acetylcystein on Antibiofilm Efficiency of Antibiotics in Staphylococci Isolates].

Mikrobiyol Bul 2021 Apr;55(2):125-145

Trakya University Faculty of Medicine, Department of Medical Microbiology, Edirne, Turkey.

Biofilms are often responsible for the difficulties in the treatment of infectious diseases due to their properties that facilitate escape from antibiotic effect and their antiphagocytic effects. At least 65% of all infectious diseases are associated with biofilm-forming bacteria. As Staphylococcus aureus and Staphylococcus epidermidis are among the most common agents of hospital infections and the infections are mostly biofilm-related, they pose an important problem. Read More

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Antibacterial Albumin-Tannic Acid Coatings for Scaffold-Guided Breast Reconstruction.

Front Bioeng Biotechnol 2021 31;9:638577. Epub 2021 Mar 31.

Centre in Regenerative Medicine, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD, Australia.

Infection is the major cause of morbidity after breast implant surgery. Biodegradable medical-grade polycaprolactone (mPCL) scaffolds designed and rooted in evidence-based research offer a promising alternative to overcome the limitations of routinely used silicone implants for breast reconstruction. Nevertheless, as with any implant, biodegradable scaffolds are susceptible to bacterial infection too, especially as bacteria can rapidly colonize the biomaterial surface and form biofilms. Read More

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Novel treatment dynamics for biofilm-related infections.

Jose L Del Pozo

Expert Rev Anti Infect Ther 2021 Apr 22:1-14. Epub 2021 Apr 22.

Infectious Diseases Division, Clínica Universidad De Navarra, Pamplona, Spain.

: As a result of progress in medical care, a huge number of medical devices are used in the treatment of human diseases. In turn, biofilm-related infection has become a growing threat due to the tolerance of biofilms to antimicrobials, a problem magnified by the development of antimicrobial resistance worldwide. As a result, successful treatment of biofilm-disease using only antimicrobials is problematic. Read More

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SC5005 dissipates the membrane potential to kill Staphylococcus aureus persisters without detectable resistance.

J Antimicrob Chemother 2021 Jul;76(8):2049-2056

Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, Taipei 10048, Taiwan.

Objectives: In the past few decades, multiple-antibiotic-resistant Staphylococcus aureus has emerged and quickly spread in hospitals and communities worldwide. Additionally, the formation of antibiotic-tolerant persisters and biofilms further reduces treatment efficacy. Previously, we identified a sorafenib derivative, SC5005, with bactericidal activity against MRSA in vitro and in vivo. Read More

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The antimicrobial peptide TAT-RasGAP inhibits the formation and expansion of bacterial biofilms in vitro.

J Glob Antimicrob Resist 2021 06 20;25:227-231. Epub 2021 Apr 20.

Institute of Microbiology, Lausanne University Hospital and University of Lausanne, Rue du Bugnon 48, 1011 Lausanne, Switzerland. Electronic address:

Objectives: Biofilms are structured aggregates of bacteria embedded in a self-produced matrix that develop in diverse ecological niches. Pathogenic bacteria can form biofilms on surfaces and in tissues, causing nosocomial and chronic infections that are difficult to treat. While antibiotics are largely inefficient in limiting biofilm formation and expansion, antimicrobial peptides (AMPs) are emerging as alternative antibiofilm treatments. Read More

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On-demand pH-sensitive surface charge-switchable polymeric micelles for targeting Pseudomonas aeruginosa biofilms development.

J Nanobiotechnology 2021 Apr 9;19(1):99. Epub 2021 Apr 9.

School of Pharmacy, Shandong New Drug Loading & Release Technology and Preparation Engineering Laboratory, Binzhou Medical University, 346 Guanhai Road, Yantai, 264003, People's Republic of China.

Bacterial biofilm is the complicated clinical issues, which usually results in bacterial resistance and reduce the therapeutic efficacy of antibiotics. Although micelles have been drawn attention in treatment of the biofilms, the micelles effectively permeate and retain in biofilms still facing a big challenge. In this study, we fabricated on-demand pH-sensitive surface charge-switchable azithromycin (AZM)-encapsulated micelles (denoted as AZM-SCSMs), aiming to act as therapeutic agent for treating Pseudomonas aeruginosa (P. Read More

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