36,731 results match your criteria biofilm formation

Microbial corrosion of DSS 2205 in an acidic chloride environment under continuous flow.

PLoS One 2021 12;16(5):e0251524. Epub 2021 May 12.

Energy and Resources Institute, College of Engineering, Information Technology and Environment, Charles Darwin University, Darwin, Northern Territory, Australia.

Corrosion under flow conditions is a major problem in the transportation industry. Various studies have shown the direct impact of different flow rates on bacteria biofilm formation, mass transfer and resulting different corrosion behaviour of materials in neutral environments. However, little is understood on corrosion under acidic flow conditions. Read More

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Cytotoxic effect of galvanically coupled magnesium-titanium particles on Escherichia coli.

J Biomed Mater Res B Appl Biomater 2021 May 12. Epub 2021 May 12.

Department of Biomedical and Chemical Engineering, College of Engineering and Computer Science, Syracuse University, Syracuse, New York, USA.

Orthopedic device-related infections (ODRIs) are difficult to control due to microbial biofilm formation and associated with high-level resistance to conventional antibiotics. In many cases, the only treatment option for ODRI is explantation. Previous studies have shown that application of cathodic potentials at the metal surface can eradicate biofilms, and Mg and Mg-Ti particles have the same effect as cathodic potentials. Read More

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Transient measurement of kombucha biofilm growth and mechanical properties.

Food Funct 2021 May;12(9):4015-4020

Institute of Food Nutrition and Health, ETH Zurich, 8092 Zurich, Switzerland.

Kombucha is a traditional beverage obtained by the fermentation of sugared tea by a symbiotic culture of bacteria and yeast which has recently re-emerged as a popular lifestyle product with potential health benefits. The characteristic feature of kombucha is the formation of a cellulosic biofilm due to the excretion of bacterial cellulose with high purity and crystallinity. Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition. Read More

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Role of D-alanylation of Streptococcus mutans Lipoteichoic Acid in Interspecies Competitiveness.

Mol Oral Microbiol 2021 May 12. Epub 2021 May 12.

Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China.

Background: The D-alanylation of lipoteichoic acid (LTA) is essential for the physiological metabolism of Streptococcus mutans (S. mutans). This study was designed to investigate the influence of D-alanylation of LTA on interspecies competitiveness of S. Read More

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Characterization of R-pyocin activity against Gram-positive pathogens for the first time with special focus on Staphylococcus aureus.

J Appl Microbiol 2021 May 11. Epub 2021 May 11.

Department of Microbiology and Botany, Faculty of Science, Zagazig University, 44519, Zagazig, Egypt.

Aim: This study is aimed at characterization of both antimicrobial and anti-biofilm activity of R-pyocin from clinical Pseudomonas aeruginosa against Gram-positive pathogens including Staphylococcus aureus.

Methods And Results: Pyocinogenic P. aeruginosa was detected using reverse-side method and pyocinogeny typing was confirmed using revised-spotting method. Read More

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Ultra-Conformable Ionic Skin with Multi-Modal Sensing, Broad-Spectrum Antimicrobial and Regenerative Capabilities for Smart and Expedited Wound Care.

Adv Sci (Weinh) 2021 05 15;8(9):2004627. Epub 2021 Feb 15.

Orthopedic Institute and Department of Orthopedics The First Affiliated Hospital, Soochow University Suzhou Jiangsu 215006 P. R. China.

While rapid wound healing is essential yet challenging, there is also an unmet need for functional restoration of sensation. Inspired by natural skin, an ultra-conformable, adhesive multi-functional ionic skin (MiS) with multi-modal sensing capability is devised for smart and expedited wound care. The base of MiS is a unique skin-like, conductive and self-adaptive adhesive polyacrylamide/starch double-network hydrogel (PSH) and self-powered, flexible, triboelectric sensor(s) is integrated on top of PSH for multi-tactile sensing. Read More

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Sustained release of usnic acid from graphene coatings ensures long term antibiofilm protection.

Sci Rep 2021 May 11;11(1):9956. Epub 2021 May 11.

Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 41296, Göteborg, Sweden.

Protecting surfaces from bacterial colonization and biofilm development is an important challenge for the medical sector, particularly when it comes to biomedical devices and implants that spend longer periods in contact with the human body. A particularly difficult challenge is ensuring long-term protection, which is usually attempted by ensuring sustained release of antibacterial compounds loaded onto various coatings. Graphene have a considerable potential to reversibly interact water insoluble molecules, which makes them promising cargo systems for sustained release of such compounds. Read More

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Identification of c-di-GMP/FleQ-Regulated New Target Genes, Including , Encoding Adenylate Cyclase, in Pseudomonas putida.

mSystems 2021 May 11;6(3). Epub 2021 May 11.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China

The bacterial second messenger cyclic diguanylate (c-di-GMP) modulates plankton-to-biofilm lifestyle transition of species through its transcriptional regulatory effector FleQ. FleQ regulates transcription of biofilm- and flagellum-related genes in response to c-di-GMP. Through transcriptomic analysis and FleQ-DNA binding assay, this study identified five new target genes of c-di-GMP/FleQ in , including , , (), (), and Except encoding an outer membrane pore protein and encoding an adenylate cyclase, the functions of the other three genes encoding hypothetical proteins remain unknown. Read More

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Evaluation of antimicrobial potential and surface morphology in thin films of titanium nitride and calcium phosphate on orthodontic brackets.

Am J Orthod Dentofacial Orthop 2021 May 8. Epub 2021 May 8.

Department of Prosthodontics, Graduate Program in Clinical Dentistry, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil.

Introduction: The goal of this research was to experimentally evaluate the surface morphology and adhesion capacity of Streptococcus mutans (U159) on brackets with thin films of titanium nitride (TN) and of titanium nitride doped with calcium phosphate (TNCP).

Methods: Twenty-four metallic brackets were equally allocated to 3 groups (n = 8), according to the type of covering (no covering, TNCP, and TN). The coatings were deposited by cathodic cage (TNCP and TN groups) and were evaluated by scanning electron microscopy and energy dispersive x-ray spectrometry. Read More

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Catalytic Cooperation between a Copper Oxide Electrocatalyst and a Microbial Community for Microbial Electrosynthesis.

Chempluschem 2021 May;86(5):763-777

Environmental Technology, Wageningen University & Research, Bornse Weilanden 9, 6708 WG, Wageningen, The Netherlands.

Electrocatalytic metals and microorganisms can be combined for CO conversion in microbial electrosynthesis (MES). However, a systematic investigation on the nature of interactions between metals and MES is still lacking. To investigate this nature, we integrated a copper electrocatalyst, converting CO to formate, with microorganisms, converting CO to acetate. Read More

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Synthesis of the fungal macrolide berkeleylactone A and its inhibition of microbial biofilm formation.

Org Biomol Chem 2021 May 11. Epub 2021 May 11.

Department of Chemistry, University Bayreuth, Universitaetsstr. 30, D-95440 Bayreuth, Germany.

The fungal macrolide berkeleylactone A was synthesised in 13 steps and 24% yield using (R)-propylene oxide and an asymmetric Noyori hydrogenation of a β-ketoester to install the stereogenic centres. A domino addition-Wittig olefination of a 13-hydroxytetradecanal intermediate with the cumulated ylide Ph3PCCO closed the macrocyle by establishing the α,β-unsaturated ester group, necessary for the attachment of the sidechain thiol via a thia-Michael reaction. The synthetic berkeleylactone A inhibited the formation of Staphylococcus aureus biofilms and showed significant dispersive effects on preformed biofilms of Candida albicans by at least 45% relative to untreated controls at concentrations as low as 1. Read More

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Bacterial Biofilms on Tracheostomy Tubes.

Indian J Otolaryngol Head Neck Surg 2021 May 6:1-5. Epub 2021 May 6.

Department of Otorhinolaryngology, Yenepoya Medical College, Mangalore, Karnataka India.

Tracheostomy is a commonly performed airway surgery for critically ill patients. Tracheostomy tube is an indwelling prosthesis, providing potential surface for growth of bacteria. Biofilm formation by bacteria as a self-protective mechanism, has led to worrisome antibacterial resistance and thus higher rate of nosocomial infections. Read More

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The evolution and changing ecology of the African hominid oral microbiome.

Proc Natl Acad Sci U S A 2021 May;118(20)

Department of Humanities, University of Ferrara, 44121 Ferrara, Italy.

The oral microbiome plays key roles in human biology, health, and disease, but little is known about the global diversity, variation, or evolution of this microbial community. To better understand the evolution and changing ecology of the human oral microbiome, we analyzed 124 dental biofilm metagenomes from humans, including Neanderthals and Late Pleistocene to present-day modern humans, chimpanzees, and gorillas, as well as New World howler monkeys for comparison. We find that a core microbiome of primarily biofilm structural taxa has been maintained throughout African hominid evolution, and these microbial groups are also shared with howler monkeys, suggesting that they have been important oral members since before the catarrhine-platyrrhine split ca. Read More

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Cell-free expression of the outer membrane protein OprF of for vaccine purposes.

Life Sci Alliance 2021 Jun 10;4(6). Epub 2021 May 10.

TheREx and Synabi, University Grenoble Alpes, CNRS, Centre Hospitalier Universitaire Grenoble Alpes, Grenoble Institut Polytechnique (INP), Translational Innovation in Medicine and Complexity (TIMC), Grenoble, France

is the second-leading cause of nosocomial infections and pneumonia in hospitals. Because of its extraordinary capacity for developing resistance to antibiotics, treating infections by is becoming a challenge, lengthening hospital stays, and increasing medical costs and mortality. The outer membrane protein OprF is a well-conserved and immunogenic porin playing an important role in quorum sensing and in biofilm formation. Read More

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Discovery of myricetin as an inhibitor against Streptococcus mutans and an anti-adhesion approach to biofilm formation.

Int J Med Microbiol 2021 May 5;311(4):151512. Epub 2021 May 5.

Center of Stomatology, Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology, No. 1095, Jiefang Road, Wuhan, Hubei, People's Republic of China; School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China. Electronic address:

Streptococcus mutans (S. mutans) are cariogenic microorganisms. Sortase A (SrtA) is a transpeptidase that attaches Pac to the cell surface. Read More

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Iodinated disinfection byproduct formation in a MnO/I/EPS system.

Chemosphere 2021 May 4;280:130643. Epub 2021 May 4.

Faculty of Environmental Engineering, University of Kitakyushu, 1-1 Hibikino, Wakamatsu, Kitakyushu, Fukuoka, Japan. Electronic address:

Manganese dioxide (MnO) is a Mn deposit widely accumulated in the corrosion layer of pipelines, and iodide (I) is a halogen ion frequently detected in waters. The biofilm dwelling on the corrosion scales often secretes extracellular polymeric substances (EPS) into drinking water. The paper aimed to study the I oxidation by MnO and iodinated disinfection byproducts (I-DBPs) formation with biofilm EPS as a precursor. Read More

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Accelerated start-up for photo-fermentative hydrogen production in biofilm reactor by adding waste effluent.

Environ Res 2021 May 7;198:111221. Epub 2021 May 7.

State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, P.O. Box 2614, 73 Huanghe Road, Harbin, 150090, China. Electronic address:

The difficulty and long duration of start-up wastes numerous costs, labors and time and a little fluctuate during the process might fail it. However, studies dealing with the problem hindering accelerated start-up are still insufficient. Current research focused to develop a method for accelerated start-up in an efficient way. Read More

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Antimicrobial properties of Lactobacillus cell-free supernatants against multidrug-resistant urogenital pathogens.

Microbiologyopen 2021 Feb;10(2):e1173

Department of Biomedical and Biotechnological Sciences, Microbiology Section, University of Catania, Catania, Italy.

The healthy vaginal microbiota is dominated by Lactobacillus spp., which provide an important critical line of defense against pathogens, as well as giving beneficial effects to the host. We characterized L. Read More

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February 2021

[Infections of the spine : Pyogenic spondylodiscitis and implant-associated vertebral osteomyelitis].

Unfallchirurg 2021 May 10. Epub 2021 May 10.

Klinik und Poliklinik für Unfallchirurgie, Universitätsklinikum Regensburg (UKR), Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Deutschland.

Pyogenic (unspecific) spondylodiscitis and implant-associated vertebral osteomyelitis (IAVO) are important diseases with the risk of neurological and septic complications. An early diagnosis is essential in which magnetic resonance imaging (MRI), histopathology and microbiological identification of the pathogen play key roles. The goals of conservative treatment in uncomplicated spondylodiscitis are antibiotic infection control and pain management. Read More

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Potential prebiotic substrates modulate composition, metabolism, virulence and inflammatory potential of an in vitro multi-species oral biofilm.

J Oral Microbiol 2021 Apr 20;13(1):1910462. Epub 2021 Apr 20.

Department of Oral Health Sciences, University of Leuven (KU Leuven), Leuven, Belgium.

: Modulation of the commensal oral microbiota constitutes a promising preventive/therapeutic approach in oral healthcare. The use of prebiotics for maintaining/restoring the health-associated homeostasis of the oral microbiota has become an important research topic. : This study hypothesised that in vitro 14-species oral biofilms can be modulated by (in)direct stimulation of beneficial/commensal bacteria with new potential prebiotic substrates tested at 1 M and 1%, resulting in more host-compatible biofilms with fewer pathogens, decreased virulence and less inflammatory potential. Read More

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Multiple CheY Proteins Control Surface-Associated Lifestyles of .

Front Microbiol 2021 22;12:664826. Epub 2021 Apr 22.

Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, TN, United States.

Bacterial chemotaxis is the directed movement of motile bacteria in gradients of chemoeffectors. This behavior is mediated by dedicated signal transduction pathways that couple environment sensing with changes in the direction of rotation of flagellar motors to ultimately affect the motility pattern. uses two distinct chemotaxis pathways, named Che1 and Che4, and four different response regulators (CheY1, CheY4, CheY6, and CheY7) to control the swimming pattern during chemotaxis. Read More

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Azithromycin Exhibits Activity Against in Chronic Rat Lung Infection Model.

Front Microbiol 2021 23;12:603151. Epub 2021 Apr 23.

Department of Microbiology, Daiichi Sankyo India Pharma Private Limited, Gurgaon, India.

forms biofilms in the lungs of chronically infected cystic fibrosis patients, which are tolerant to both the treatment of antibiotics and the host immune system. Normally, antibiotics are less effective against bacteria growing in biofilms; azithromycin has shown a potent efficacy in cystic fibrosis patients chronically infected with and improved their lung function. The present study was conducted to evaluate the effect of azithromycin on biofilm. Read More

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Bioinspired double self-adhesion coating based on dopamine, coating resin and phosphorylcholine for surface lubrication and antifouling functionalization.

Des Monomers Polym 2021 Apr 22;24(1):106-112. Epub 2021 Apr 22.

School of Chemical Engineering and Technology, Tianjin University, Tianjin, P. R. China.

Implanted medical devices that have poor friction property or biofilm formation can limit their service life and cause discomfort in patients. Recently, some zwitterionic coatings have been studied to modify the biomaterials surface for lubricating function, but the grafting methods of coatings are complicated and also seldom take the bacterial antiadhesion property into account at the same time. In our studies, motivated by the properties of nature mussels and human articular, we firstly successfully synthesized double adhesion protection of self-adhesive ternary polymer coating and achieved the excellent lubrication and antifouling functionalization of the medical devices surface. Read More

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Activity of Biodegradable Polymeric Nanosponges against Dual-Species Bacterial Biofilms.

ACS Biomater Sci Eng 2021 May 1;7(5):1780-1786. Epub 2020 Dec 1.

Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.

Infections caused by multidrug-resistant (MDR) bacteria present an emerging global health crisis, and the threat is intensified by the involvement of biofilms. Some biofilm infections involve more than one species; this can further challenge treatment using traditional antibiotics. Nanomaterials are being developed as alternative therapeutics to traditional antibiotics; here we report biodegradable polymer-stabilized oil-in-water nanosponges (BNS) and show their activity against dual-species bacterial biofilms. Read More

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Antimicrobial and enzyme-responsive multi-peptide surfaces for bone-anchored devices.

Mater Sci Eng C Mater Biol Appl 2021 Jun 16;125:112108. Epub 2021 Apr 16.

MDRCBB-Minnesota Dental Research Center for Biomaterials and Biomechanics, University of Minnesota, Moos Tower, 515 Delaware St. SE, Minneapolis, MN 55455, USA. Electronic address:

Functionalization of dental and orthopedic implants with multiple bioactivities is desirable to obtain surfaces with improved biological performance and reduced infection rates. While many approaches have been explored to date, nearly all functionalized surfaces are static, i.e. Read More

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The gold nanoparticle reduces Vibrio cholerae pathogenesis by inhibition of biofilm formation and disruption of the production and structure of cholera toxin.

Colloids Surf B Biointerfaces 2021 Apr 30;204:111811. Epub 2021 Apr 30.

Department of Biochemistry, Bose Institute, P1/12 CIT Scheme VIIM, Kolkata, 700054, India.

Formation of biofilm by Vibrio cholerae plays a crucial role in pathogenesis and transmission of cholera. Lower infective dose of the biofilm form of V. cholerae compared to the planktonic counterpart, and its antibiotic resistance, make it challenging to combat cholera. Read More

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Ferrocene-functionalized hybrid hydrogel dressing with high-adhesion for combating biofilm.

Mater Sci Eng C Mater Biol Appl 2021 Jun 18;125:112111. Epub 2021 Apr 18.

Department of Microbiology, College of Life Science, Hunan Normal University, Changsha 410081, Hunan, People's Republic of China; State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha 410081, Hunan, People's Republic of China. Electronic address:

Bacterial infection is a common phenomenon in the process of postoperative wound healing. In severe cases, it may even lead to life-threatening, which brings a heavy burden to the clinical treatment and causes huge losses to the society and economy. As one of the most commonly applied medical materials for wound treatment, hydrogel dressings are mainly used to cover and protect wounds and provide a favorable environment to facilitate wound healing. Read More

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Iron oxide minerals promote simultaneous bio-reduction of Cr(VI) and nitrate: Implications for understanding natural attenuation.

Sci Total Environ 2021 Apr 29;786:147396. Epub 2021 Apr 29.

School of Water Resources and Environment, MOE Key Laboratory of Groundwater Circulation and Environmental Evolution, China University of Geosciences (Beijing), Beijing 100083, PR China.

Nitrate and Cr(VI) coexist in aquifers, posing a potential threat to ecological environment and public health. Iron oxide minerals (hematite and magnetite) exist ubiquitously in groundwater, which are hot spots for biogeochemical transformation. However, there is still a knowledge gap anout the effect of iron oxide minerals on bioreduction of nitrate and Cr(VI). Read More

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Application of an indigenous microorganisms-based fixed-bed GAC-biofilm reactor for passive and sustainable treatment of oil sands process water through combined adsorption and biodegradation processes.

Chemosphere 2021 Apr 30;280:130635. Epub 2021 Apr 30.

Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta, T6G 1H9, Canada. Electronic address:

In this study, a fixed-bed biofilm reactor (biofilter) was developed and applied for oil sands process water (OSPW) remediation by using granular activated carbon (GAC) as packing media. Using quantitative polymerase chain reaction (qPCR) detection, the total bacterial copy number (16S) in the GAC biofiltration system was found to reach a relatively stable level (1.3 ± 0. Read More

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Design, synthesis and evaluation of novel 9-arylalkyl-10-methylacridinium derivatives as highly potent FtsZ-targeting antibacterial agents.

Eur J Med Chem 2021 Apr 28;221:113480. Epub 2021 Apr 28.

Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, 44 West Wenhua Road, Jinan, 250012, China. Electronic address:

With the increasing incidence of antibiotic resistance, new antibacterial agents having novel mechanisms of action hence are in an urgent need to combat infectious diseases caused by multidrug-resistant (MDR) pathogens. Four novel series of substituted 9-arylalkyl-10-methylacridinium derivatives as FtsZ inhibitors were designed, synthesized and evaluated for their antibacterial activities against various Gram-positive and Gram-negative bacteria. The results demonstrated that they exhibited broad-spectrum activities with substantial efficacy against MRSA and VRE, which were superior or comparable to the berberine, sanguinarine, linezolid, ciprofloxacin and vancomycin. Read More

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