59,670 results match your criteria biofilm

Microbial corrosion of DSS 2205 in an acidic chloride environment under continuous flow.

PLoS One 2021 12;16(5):e0251524. Epub 2021 May 12.

Energy and Resources Institute, College of Engineering, Information Technology and Environment, Charles Darwin University, Darwin, Northern Territory, Australia.

Corrosion under flow conditions is a major problem in the transportation industry. Various studies have shown the direct impact of different flow rates on bacteria biofilm formation, mass transfer and resulting different corrosion behaviour of materials in neutral environments. However, little is understood on corrosion under acidic flow conditions. Read More

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Use of low-frequency contact ultrasonic debridement with and without polyhexamethylene biguanide in hard-to-heal leg ulcers: an RCT protocol.

J Wound Care 2021 May;30(5):372-379

School of Nursing, Midwifery and Paramedicine, The University of the Sunshine Coast, Locked Bag 4, Maroochydore, QLD 4558, Australia.

Objective: The purpose of this research is to investigate the effect of low-frequency contact ultrasonic debridement therapy (LFCUD) in hard-to-heal wounds with suspected biofilm, and compare the effect with or without a surfactant antimicrobial on bacterial colony counts and wound healing rates.

Method: A single-blinded randomised controlled trial (RCT) will investigate the combination of LFCUD and the antiseptic polyhexamethylene biguanide with a surfactant betaine (referred to in this paper as PHMB) as a topical solution post-treatment and in a sustained dressing, compared with use of LFCUD alone. Potential participants from a community wound clinic (n=50) will be invited to take part in the 12-week trial. Read More

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An audit to assess the impact of prescribing a monofilament fibre debridement pad for patients with unhealed wounds after six months.

J Wound Care 2021 May;30(5):381-388

Lohmann & Rauscher, Burton on Trent, Staffordshire, UK.

A monofilament fibre debridement pad has been found to be a rapid and effective mechanical method of removing dry skin, biofilm and debris from acute and chronic wounds with minimal patient discomfort. Evidence of its impact on prescribing and wound healing, however, has been more limited. The aim of this audit was to show evidence of the monofilament fibre debridement pad's impact on wound treatment costs through an analysis of NHS wound-care prescribing data in England. Read More

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A new model for silicification of cyanobacteria in Proterozoic tidal flats.

Geobiology 2021 May 12. Epub 2021 May 12.

Department of Earth, Atmospheric and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA.

Microbial fossils preserved by early diagenetic chert provide a window into the Proterozoic biosphere, but seawater chemistry, microbial processes, and the interactions between microbes and the environment that contributed to this preservation are not well constrained. Here, we use fossilization experiments to explore the processes that preserve marine cyanobacterial biofilms by the precipitation of amorphous silica in a seawater medium that is analogous to Proterozoic seawater. These experiments demonstrate that the exceptional silicification of benthic marine cyanobacteria analogous to the oldest diagnostic cyanobacterial fossils requires interactions among extracellular polymeric substances (EPS), photosynthetically induced pH changes, magnesium cations (Mg ), and >70 ppm silica. Read More

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Cytotoxic effect of galvanically coupled magnesium-titanium particles on Escherichia coli.

J Biomed Mater Res B Appl Biomater 2021 May 12. Epub 2021 May 12.

Department of Biomedical and Chemical Engineering, College of Engineering and Computer Science, Syracuse University, Syracuse, New York, USA.

Orthopedic device-related infections (ODRIs) are difficult to control due to microbial biofilm formation and associated with high-level resistance to conventional antibiotics. In many cases, the only treatment option for ODRI is explantation. Previous studies have shown that application of cathodic potentials at the metal surface can eradicate biofilms, and Mg and Mg-Ti particles have the same effect as cathodic potentials. Read More

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Tobramycin Liquid Crystal Nanoparticles Eradicate Cystic Fibrosis-Related Pseudomonas aeruginosa Biofilms.

Small 2021 May 12:e2100531. Epub 2021 May 12.

Clinical and Health Science, University of South Australia, North Tce, Adelaide, SA, 5000, Australia.

Pseudomonas aeruginosa biofilms cause persistent and chronic infections, most known clinically in cystic fibrosis (CF). Tobramycin (TOB) is a standard anti-pseudomonal antibiotic; however, in biofilm infections, its efficacy severely decreases due to limited permeability across the biofilm matrix. Herewith, a biomimetic, nanostructured, lipid liquid crystal nanoparticle-(LCNP)-formulation is discovered to significantly enhance the efficacy of TOB and eradicate P. Read More

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Anti biofilm effect of low concentration chlorine containing disinfectant assisted by multi enzyme detergent in dental unit waterlines.

New Microbiol 2021 May 1;44(2). Epub 2021 May 1.

The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, Zhejiang, China.

The highly structured biofilms on the surface of internal tubing are regarded as the most important source of water pollution in the dental unit waterline ( DUWL )). Herein, the study aim ed to evaluate the anti biofilm effect of combined application of chlorine containing disinfectant with multi enzyme detergent in the dental unit waterline. Six dental units were included and randomly divided into two groups Group A was treated with chlorine containing disinfectant and multi enzyme detergent; Group Bwa s treated only with chlorine containing disinfectant as control. Read More

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Transient measurement of kombucha biofilm growth and mechanical properties.

Food Funct 2021 May;12(9):4015-4020

Institute of Food Nutrition and Health, ETH Zurich, 8092 Zurich, Switzerland.

Kombucha is a traditional beverage obtained by the fermentation of sugared tea by a symbiotic culture of bacteria and yeast which has recently re-emerged as a popular lifestyle product with potential health benefits. The characteristic feature of kombucha is the formation of a cellulosic biofilm due to the excretion of bacterial cellulose with high purity and crystallinity. Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition. Read More

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pH-Triggered nanoreactors as oxidative stress amplifiers for combating multidrug-resistant biofilms.

Chem Commun (Camb) 2021 May;57(38):4662-4665

Department of Clinical Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. and Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Developing radical oxygen species (ROS)-generating nanoreactors as new "antibiotics" is a promising strategy for the treatment of multidrug-resistant (MDR) biofilm infections. Herein, we designed and fabricated silver nanoparticle-decorated calcium peroxide (CaO2) nanoreactors (CPA) for combating MDR biofilms. CPA could locally boost ROS production as oxidative stress amplifiers in a pH-triggered and self-catalytic manner in acidic biofilms, where H2O2 was released by the hydrolysis of CaO2 and sequentially catalyzed by Ag NPs in situ to generate O2˙-, thereby efficiently disrupting mature biofilms and killing bacteria. Read More

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Role of D-alanylation of Streptococcus mutans Lipoteichoic Acid in Interspecies Competitiveness.

Mol Oral Microbiol 2021 May 12. Epub 2021 May 12.

Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China.

Background: The D-alanylation of lipoteichoic acid (LTA) is essential for the physiological metabolism of Streptococcus mutans (S. mutans). This study was designed to investigate the influence of D-alanylation of LTA on interspecies competitiveness of S. Read More

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Characterization of R-pyocin activity against Gram-positive pathogens for the first time with special focus on Staphylococcus aureus.

J Appl Microbiol 2021 May 11. Epub 2021 May 11.

Department of Microbiology and Botany, Faculty of Science, Zagazig University, 44519, Zagazig, Egypt.

Aim: This study is aimed at characterization of both antimicrobial and anti-biofilm activity of R-pyocin from clinical Pseudomonas aeruginosa against Gram-positive pathogens including Staphylococcus aureus.

Methods And Results: Pyocinogenic P. aeruginosa was detected using reverse-side method and pyocinogeny typing was confirmed using revised-spotting method. Read More

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Treatment of mustard tuber wastewater (MTWW) using a pilot-scale packed cage rotating biological contactor system: process modeling and optimization.

Environ Sci Pollut Res Int 2021 May 12. Epub 2021 May 12.

National Base of International Science and Technology Cooperation for Intelligent Manufacturing Service, Chongqing Technology and Business University, Chongqing, 400067, China.

The water quality range for wastewater treatment projects in the food processing industry changes constantly. To fully understand the threshold for pollutant removal with the lowest possible energy consumption, the relationship between pollutant removal and wastewater treatment conditions was established using response surface methodology (RSM). The optimum conditions for total COD, TN, and NH-N removal from saline mustard tuber wastewater (MTWW) with a packed cage rotating biological contactor (RBC) system were investigated by experiments based on a Box-Behnken design (BBD). Read More

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Ultra-Conformable Ionic Skin with Multi-Modal Sensing, Broad-Spectrum Antimicrobial and Regenerative Capabilities for Smart and Expedited Wound Care.

Adv Sci (Weinh) 2021 05 15;8(9):2004627. Epub 2021 Feb 15.

Orthopedic Institute and Department of Orthopedics The First Affiliated Hospital, Soochow University Suzhou Jiangsu 215006 P. R. China.

While rapid wound healing is essential yet challenging, there is also an unmet need for functional restoration of sensation. Inspired by natural skin, an ultra-conformable, adhesive multi-functional ionic skin (MiS) with multi-modal sensing capability is devised for smart and expedited wound care. The base of MiS is a unique skin-like, conductive and self-adaptive adhesive polyacrylamide/starch double-network hydrogel (PSH) and self-powered, flexible, triboelectric sensor(s) is integrated on top of PSH for multi-tactile sensing. Read More

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Sustained release of usnic acid from graphene coatings ensures long term antibiofilm protection.

Sci Rep 2021 May 11;11(1):9956. Epub 2021 May 11.

Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 41296, Göteborg, Sweden.

Protecting surfaces from bacterial colonization and biofilm development is an important challenge for the medical sector, particularly when it comes to biomedical devices and implants that spend longer periods in contact with the human body. A particularly difficult challenge is ensuring long-term protection, which is usually attempted by ensuring sustained release of antibacterial compounds loaded onto various coatings. Graphene have a considerable potential to reversibly interact water insoluble molecules, which makes them promising cargo systems for sustained release of such compounds. Read More

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Identification of c-di-GMP/FleQ-Regulated New Target Genes, Including , Encoding Adenylate Cyclase, in Pseudomonas putida.

mSystems 2021 May 11;6(3). Epub 2021 May 11.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China

The bacterial second messenger cyclic diguanylate (c-di-GMP) modulates plankton-to-biofilm lifestyle transition of species through its transcriptional regulatory effector FleQ. FleQ regulates transcription of biofilm- and flagellum-related genes in response to c-di-GMP. Through transcriptomic analysis and FleQ-DNA binding assay, this study identified five new target genes of c-di-GMP/FleQ in , including , , (), (), and Except encoding an outer membrane pore protein and encoding an adenylate cyclase, the functions of the other three genes encoding hypothetical proteins remain unknown. Read More

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Evaluation of antimicrobial potential and surface morphology in thin films of titanium nitride and calcium phosphate on orthodontic brackets.

Am J Orthod Dentofacial Orthop 2021 May 8. Epub 2021 May 8.

Department of Prosthodontics, Graduate Program in Clinical Dentistry, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil.

Introduction: The goal of this research was to experimentally evaluate the surface morphology and adhesion capacity of Streptococcus mutans (U159) on brackets with thin films of titanium nitride (TN) and of titanium nitride doped with calcium phosphate (TNCP).

Methods: Twenty-four metallic brackets were equally allocated to 3 groups (n = 8), according to the type of covering (no covering, TNCP, and TN). The coatings were deposited by cathodic cage (TNCP and TN groups) and were evaluated by scanning electron microscopy and energy dispersive x-ray spectrometry. Read More

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An orthobiologics-free strategy for synergistic photocatalytic antibacterial and osseointegration.

Biomaterials 2021 May 4;274:120853. Epub 2021 May 4.

Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, PR China; Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang Province, PR China; Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang Province, PR China. Electronic address:

Tissue damage caused by hyperthermia during photothermal therapy (PTT) has largely limited its clinical applications for implant infection. However, rescue of tissue regeneration by conjugating orthobiologics with PTT has been problematic as they can easily deactivate biologics while eradicating bacteria. Herein, we report an orthobiologics-free strategy to synergistically couple photocatalytic antibacterial with pro-osteogenic capacity via self-assembly of copper sulphide nanoparticle (CuS NP) and reduced graphene oxide (rGO) on implant surface. Read More

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Carrier type induces anammox biofilm structure and the nitrogen removal pathway: Demonstration in a full-scale partial nitritation/anammox process.

Bioresour Technol 2021 May 4;334:125249. Epub 2021 May 4.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China. Electronic address:

In this study, two typical carrier types, microporous and macroporous carriers, were collected from a full-scale partial nitritation/anammox reactor for analysis and comparison of the biofilm structure characteristics, performance and removal nitrogen pathway. For microporous carriers, a thicker biofilm (>5 mm) was obtained with higher biomass and abundance of anammox bacteria as well as a higher nitrogen removal efficiency due to the integration of denitrifying and anammox bacteria. In addition, higher microbial community stability can be expected under varying environmental conditions. Read More

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ATP-dependent protease ClpP and its subunits ClpA, ClpB, and ClpX involved in the field bismerthiazol-resistance in pv. .

Phytopathology 2021 May 11. Epub 2021 May 11.

Plant Protection, Nanjing, Jiangsu, China;

Resistance of pv. (), which causes rice bacterial leaf blight, to bismerthiazol has been detected in China since the 1990s. Interestingly, the strains resistant to bismerthiazol on rice plants were more sensitive to bismerthiazol than wild-type (WT) strains . Read More

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Catalytic Cooperation between a Copper Oxide Electrocatalyst and a Microbial Community for Microbial Electrosynthesis.

Chempluschem 2021 May;86(5):763-777

Environmental Technology, Wageningen University & Research, Bornse Weilanden 9, 6708 WG, Wageningen, The Netherlands.

Electrocatalytic metals and microorganisms can be combined for CO conversion in microbial electrosynthesis (MES). However, a systematic investigation on the nature of interactions between metals and MES is still lacking. To investigate this nature, we integrated a copper electrocatalyst, converting CO to formate, with microorganisms, converting CO to acetate. Read More

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Synthesis of the fungal macrolide berkeleylactone A and its inhibition of microbial biofilm formation.

Org Biomol Chem 2021 May 11. Epub 2021 May 11.

Department of Chemistry, University Bayreuth, Universitaetsstr. 30, D-95440 Bayreuth, Germany.

The fungal macrolide berkeleylactone A was synthesised in 13 steps and 24% yield using (R)-propylene oxide and an asymmetric Noyori hydrogenation of a β-ketoester to install the stereogenic centres. A domino addition-Wittig olefination of a 13-hydroxytetradecanal intermediate with the cumulated ylide Ph3PCCO closed the macrocyle by establishing the α,β-unsaturated ester group, necessary for the attachment of the sidechain thiol via a thia-Michael reaction. The synthetic berkeleylactone A inhibited the formation of Staphylococcus aureus biofilms and showed significant dispersive effects on preformed biofilms of Candida albicans by at least 45% relative to untreated controls at concentrations as low as 1. Read More

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Bacterial Biofilms on Tracheostomy Tubes.

Indian J Otolaryngol Head Neck Surg 2021 May 6:1-5. Epub 2021 May 6.

Department of Otorhinolaryngology, Yenepoya Medical College, Mangalore, Karnataka India.

Tracheostomy is a commonly performed airway surgery for critically ill patients. Tracheostomy tube is an indwelling prosthesis, providing potential surface for growth of bacteria. Biofilm formation by bacteria as a self-protective mechanism, has led to worrisome antibacterial resistance and thus higher rate of nosocomial infections. Read More

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Numerical study of nano-biofilm stagnation flow from a nonlinear stretching/shrinking surface with variable nanofluid and bioconvection transport properties.

Sci Rep 2021 May 10;11(1):9877. Epub 2021 May 10.

Department of Mechanical/Aeronautical Engineering, Salford University, Manchester, M54WT, UK.

A mathematical model is developed for stagnation point flow toward a stretching or shrinking sheet of liquid nano-biofilm containing spherical nano-particles and bioconvecting gyrotactic micro-organisms. Variable transport properties of the liquid (viscosity, thermal conductivity, nano-particle species diffusivity) and micro-organisms (species diffusivity) are considered. Buongiorno's two-component nanoscale model is deployed and spherical nanoparticles in a dilute nanofluid considered. Read More

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The evolution and changing ecology of the African hominid oral microbiome.

Proc Natl Acad Sci U S A 2021 May;118(20)

Department of Humanities, University of Ferrara, 44121 Ferrara, Italy.

The oral microbiome plays key roles in human biology, health, and disease, but little is known about the global diversity, variation, or evolution of this microbial community. To better understand the evolution and changing ecology of the human oral microbiome, we analyzed 124 dental biofilm metagenomes from humans, including Neanderthals and Late Pleistocene to present-day modern humans, chimpanzees, and gorillas, as well as New World howler monkeys for comparison. We find that a core microbiome of primarily biofilm structural taxa has been maintained throughout African hominid evolution, and these microbial groups are also shared with howler monkeys, suggesting that they have been important oral members since before the catarrhine-platyrrhine split ca. Read More

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Cell-free expression of the outer membrane protein OprF of for vaccine purposes.

Life Sci Alliance 2021 Jun 10;4(6). Epub 2021 May 10.

TheREx and Synabi, University Grenoble Alpes, CNRS, Centre Hospitalier Universitaire Grenoble Alpes, Grenoble Institut Polytechnique (INP), Translational Innovation in Medicine and Complexity (TIMC), Grenoble, France

is the second-leading cause of nosocomial infections and pneumonia in hospitals. Because of its extraordinary capacity for developing resistance to antibiotics, treating infections by is becoming a challenge, lengthening hospital stays, and increasing medical costs and mortality. The outer membrane protein OprF is a well-conserved and immunogenic porin playing an important role in quorum sensing and in biofilm formation. Read More

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The mobile genetic element RD2 affects colonization potential of different GAS serotypes.

Laura C Cook

Infect Immun 2021 May 10. Epub 2021 May 10.

Binghamton Biofilm Research Center, Department of Biology, Binghamton University, Binghamton, New York, USA.

M-type 28 (Group A Strep, GAS) strains are highly associated with life-threatening puerperal infections. Genome sequencing has revealed a large mobile genetic element, RD2, present in most M28 GAS isolates but not found widely in other serotypes. Previous studies have linked RD2 to the ability of M28 GAS to colonize the vaginal tract. Read More

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Photothermal nanoparticles for ablation of bacteria associated with kidney stones.

Int J Hyperthermia 2021 ;38(1):760-770

Department of Plastic and Reconstructive Surgery, Wake Forest University Health Sciences, Winston-Salem, NC, USA.

Objective: To determine whether photothermal polymer nanoparticles (NPs) can interface with bacteria associated with kidney stones, generate heat when stimulated with near infrared (NIR) light, and aid in reducing bacterial burden.

Methods: Two types of kidney stones, artificial, and those removed during percutaneous nephrolithotomy (PCNL), were inoculated with () and then incubated with NPs composed of FITC-labeled Poly[4,4-bis(2-ethylhexyl)-cyclopenta[2,1-b;3,4-b']-dithiophene-2,6-diyl-alt-2,1,3-benzoselenadiazole-4,7-diyl] (PCPDTBSe). Association of the PCPDTBSe NPs was evaluated using fluorescence microscopy. Read More

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January 2021

Discovery of myricetin as an inhibitor against Streptococcus mutans and an anti-adhesion approach to biofilm formation.

Int J Med Microbiol 2021 May 5;311(4):151512. Epub 2021 May 5.

Center of Stomatology, Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology, No. 1095, Jiefang Road, Wuhan, Hubei, People's Republic of China; School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China. Electronic address:

Streptococcus mutans (S. mutans) are cariogenic microorganisms. Sortase A (SrtA) is a transpeptidase that attaches Pac to the cell surface. Read More

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Iodinated disinfection byproduct formation in a MnO/I/EPS system.

Chemosphere 2021 May 4;280:130643. Epub 2021 May 4.

Faculty of Environmental Engineering, University of Kitakyushu, 1-1 Hibikino, Wakamatsu, Kitakyushu, Fukuoka, Japan. Electronic address:

Manganese dioxide (MnO) is a Mn deposit widely accumulated in the corrosion layer of pipelines, and iodide (I) is a halogen ion frequently detected in waters. The biofilm dwelling on the corrosion scales often secretes extracellular polymeric substances (EPS) into drinking water. The paper aimed to study the I oxidation by MnO and iodinated disinfection byproducts (I-DBPs) formation with biofilm EPS as a precursor. Read More

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Accelerated start-up for photo-fermentative hydrogen production in biofilm reactor by adding waste effluent.

Environ Res 2021 May 7:111221. Epub 2021 May 7.

State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, P.O. Box 2614, 73 Huanghe Road, Harbin 150090, China. Electronic address:

The difficulty and long duration of start-up wastes numerous costs, labors and time and a little fluctuate during the process might fail it. However, studies dealing with the problem hindering accelerated start-up are still insufficient. Current research focused to develop a method for accelerated start-up in an efficient way. Read More

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