24,965 results match your criteria bioenergetic membranes


Interaction of membrane-embedded cytochrome b-complexes with quinols: Classical Q-cycle and murburn model.

Cell Biochem Funct 2022 Jan 13. Epub 2022 Jan 13.

Karel de Grote University College, Antwerp University Association, Campus Hoboken, Hoboken, Belgium.

We recently proposed a diffusible reactive (oxygen) species (DRS/DROS) based function for cytochrome b complexes (CBC) and quinones (Q)/quinols (QH ) in the murburn model of bioenergetics. This proposal is in direct conflict with the classical purview of Q-cycle. Via extensive analyses of the structure-function correlations of membrane-quinones/quinols and proteins, we present qualitative and quantitative arguments to infer that the classical model cannot explain the energetics, kinetics, mechanism and probabilistic considerations. Read More

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January 2022

Analysis of microbial community resistance mechanisms in groundwater contaminated with SAs and high NH-Fe-Mn.

Sci Total Environ 2022 Jan 10:153036. Epub 2022 Jan 10.

Key Lab of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun 130021, People's Republic of China; College of New Energy and Environment, Jilin University, Changchun 130021, People's Republic of China; Institute of Water Resources and Environment, Jilin University, Changchun 130021, People's Republic of China.

The resistance mechanism of microbial communities in contaminated groundwater under the combined stress of sulfonamide antibiotics (SAs), NH, and Fe-Mn exceeding the standard levels was studied in an agricultural area along the Songhua River in Northeast China with developed livestock and poultry breeding. Representative points were selected in the study area to explore the response of environmental parameters and microbial communities, and microscopic experiments with different SA concentrations were conducted with background groundwater. The results showed a complex relationship between microbial communities and environmental factors. Read More

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January 2022

Anionic Lipids Confine Cytochrome to the Surface of Bioenergetic Membranes without Compromising Its Interaction with Redox Partners.

Biochemistry 2022 Jan 13. Epub 2022 Jan 13.

Theoretical and Computational Biophysics Group, NIH Center for Macromolecular Modeling and Bioinformatics, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.

Cytochrome (cyt. ) is a major element in electron transfer between redox proteins in bioenergetic membranes. While the interaction between cyt. Read More

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January 2022

The Effect of Sleep Deprivation and Subsequent Recovery Period on the Synaptic Proteome of Rat Cerebral Cortex.

Mol Neurobiol 2022 Jan 5. Epub 2022 Jan 5.

Laboratory of Proteomics, Institute of Biology, ELTE Eötvös Loránd University, Budapest, H-1117, Hungary.

Sleep deprivation (SD) is commonplace in the modern way of life and has a substantial social, medical, and human cost. Sleep deprivation induces cognitive impairment such as loss of executive attention, working memory decline, poor emotion regulation, increased reaction times, and higher cognitive functions are particularly vulnerable to sleep loss. Furthermore, SD is associated with obesity, diabetes, cardiovascular diseases, cancer, and a vast majority of psychiatric and neurodegenerative disorders are accompanied by sleep disturbances. Read More

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January 2022

Hydrogen, a Novel Therapeutic Molecule, Regulates Oxidative Stress, Inflammation, and Apoptosis.

Front Physiol 2021 20;12:789507. Epub 2021 Dec 20.

Research Center for Translational Medicine, Tongji University Affiliated East Hospital, Shanghai, China.

Molecular hydrogen (H) is a colorless and odorless gas. Studies have shown that H inhalation has the therapeutic effects in many animal studies and clinical trials, and its application is recommended in the novel coronavirus pneumonia treatment guidelines in China recently. H has a relatively small molecular mass, which helps it quickly spread and penetrate cell membranes to exert a wide range of biological effects. Read More

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December 2021

Differences in Cell-Intrinsic Inflammatory Programs of Yolk Sac and Bone Marrow Macrophages.

Cells 2021 12 17;10(12). Epub 2021 Dec 17.

Medizinische Klinik und Poliklinik I, LMU Klinikum, Ludwig-Maximilians-Universität, 81377 Munich, Germany.

Background: Tissue-resident macrophages have mixed developmental origins. They derive in variable extent from yolk sac (YS) hematopoiesis during embryonic development. Bone marrow (BM) hematopoietic progenitors give rise to tissue macrophages in postnatal life, and their contribution increases upon organ injury. Read More

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December 2021

Role of Mitochondrial Protein Import in Age-Related Neurodegenerative and Cardiovascular Diseases.

Cells 2021 12 14;10(12). Epub 2021 Dec 14.

Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia.

Mitochondria play a critical role in providing energy, maintaining cellular metabolism, and regulating cell survival and death. To carry out these crucial functions, mitochondria employ more than 1500 proteins, distributed between two membranes and two aqueous compartments. An extensive network of dedicated proteins is engaged in importing and sorting these nuclear-encoded proteins into their designated mitochondrial compartments. Read More

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December 2021

Isolation and Measurement of Respiration and Structural Studies of Purified Mitochondria from Heterotrophic Plant Tissues.

Curr Protoc 2021 Dec;1(12):e326

National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, India.

Mitochondria are the power houses of eukaryotic cells. These organelles contain various oxidoreductase complexes. Electron transfer from different reducing equivalents channeled via these complexes drives proton translocation across the inner mitochondrial membrane, leading to ATP generation. Read More

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December 2021

Reductive Stress of Sulfur-Containing Amino Acids within Proteins and Implication of Tandem Protein-Lipid Damage.

Int J Mol Sci 2021 Nov 28;22(23). Epub 2021 Nov 28.

ISOF, Consiglio Nazionale delle Ricerche, 40129 Bologna, Italy.

Reductive radical stress represents the other side of the redox spectrum, less studied but equally important compared to oxidative stress. The reactivity of hydrogen atoms (H) and hydrated electrons (e) connected with peptides/proteins is summarized, focusing on the chemical transformations of methionine (Met) and cystine (CysS-SCys) residues into α-aminobutyric acid and alanine, respectively. Chemical and mechanistic aspects of desulfurization processes with formation of diffusible sulfur-centered radicals, such as methanethiyl (CHS) and sulfhydryl (HS) radicals, are discussed. Read More

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November 2021

Blocking NHE Channels Reduces the Ability of In Vitro Capacitated Mammalian Sperm to Respond to Progesterone Stimulus.

Int J Mol Sci 2021 Nov 23;22(23). Epub 2021 Nov 23.

Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, ES-17003 Girona, Spain.

Few data exist about the presence and physiological role of Na+/H+ exchangers (NHEs) in the plasma membrane of mammalian sperm. In addition, the involvement of these channels in the ability of sperm to undergo capacitation and acrosomal reaction has not been investigated in any mammalian species. In the present study, we addressed whether these channels are implicated in these two sperm events using the pig as a model. Read More

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November 2021

Linking function to global and local dynamics in an elevator-type transporter.

Proc Natl Acad Sci U S A 2021 12;118(49)

Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY 10065;

Transporters cycle through large structural changes to translocate molecules across biological membranes. The temporal relationships between these changes and function, and the molecular properties setting their rates, determine transport efficiency-yet remain mostly unknown. Using single-molecule fluorescence microscopy, we compare the timing of conformational transitions and substrate uptake in the elevator-type transporter Glt We show that the elevator-like movements of the substrate-loaded transport domain across membranes and substrate release are kinetically heterogeneous, with rates varying by orders of magnitude between individual molecules. Read More

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December 2021

Effects of pharmaceuticals on membrane bioreactor: Review on membrane fouling mechanisms and fouling control strategies.

Sci Total Environ 2022 Feb 1;808:152132. Epub 2021 Dec 1.

Department of Civil Engineering, Indian Institute of Technology Delhi, New Delhi 110016, India. Electronic address:

Pharmaceuticals have become contaminants of emerging concern due to their toxicity towards aquatic life and pseudo persistent nature in the environment. Membrane bioreactor (MBR) is one such technology that has the potential to act as a barrier against the release of pharmaceuticals into the environment. Fouling is the deposition of the constituents of the mixed liquor on the membrane surface and it limit the world-wide applicability of MBRs. Read More

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February 2022

Insight of the role of mitochondrial calcium homeostasis in hepatic insulin resistance.

Mitochondrion 2022 01 29;62:128-138. Epub 2021 Nov 29.

Department of Occupational and Environmental Health, Dalian Medical University, 9 W Lushun South Road, Dalian 116044, PR China. Electronic address:

Due to the rapid rise in the prevalence of chronic metabolic disease, more and more clinicians and basic medical researchers focus their eyesight on insulin resistance (IR), an early and central event of metabolic diseases. The occurrence and development of IR are primarily caused by excessive energy intake and reduced energy consumption. Liver is the central organ that controls glucose homeostasis, playing a considerable role in systemic IR. Read More

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January 2022

Involvement of aldehyde oxidase in the metabolism of aromatic and aliphatic aldehyde-odorants in the mouse olfactory epithelium.

Arch Biochem Biophys 2022 01 29;715:109099. Epub 2021 Nov 29.

Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.

Xenobiotic-metabolizing enzymes (XMEs) expressed in the olfactory epithelium (OE) are known to metabolize odorants. Aldehyde oxidase (AOX) recognizes a wide range of substrates among which are substrates with aldehyde groups. Some of these AOX substrates are odorants, such as benzaldehyde and n-octanal. Read More

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January 2022

Engineered Nanoconfinement Accelerating Spontaneous Manganese-Catalyzed Degradation of Organic Contaminants.

Environ Sci Technol 2021 12 1;55(24):16708-16715. Epub 2021 Dec 1.

Department of Chemical and Environmental Engineering, Yale University, 17 Hillhouse Ave, New Haven, Connecticut 06511, United States.

Manganese(III/IV) oxide minerals are known to spontaneously degrade organic pollutants in nature. However, the kinetics are too slow to be useful for engineered water treatment processes. Herein, we demonstrate that nanoscale MnO particles under nanoscale spatial confinement (down to 3-5 nm) can significantly accelerate the kinetics of pollutant degradation, nearly 3 orders of magnitude faster compared to the same reaction in the unconfined bulk phase. Read More

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December 2021

Functional Dynamics of an Ancient Membrane-Bound Hydrogenase.

J Am Chem Soc 2021 Dec 30;143(49):20873-20883. Epub 2021 Nov 30.

Department of Biochemistry and Biophysics, Stockholm University, 10691 Stockholm, Sweden.

The membrane-bound hydrogenase (Mbh) is a redox-driven Na/H transporter that employs the energy from hydrogen gas (H) production to catalyze proton pumping and Na/H exchange across cytoplasmic membranes of archaea. Despite a recently resolved structure of this ancient energy-transducing enzyme [Yu et al. , , 1636-1649], the molecular principles of its redox-driven ion-transport mechanism remain puzzling and of major interest for understanding bioenergetic principles of early cells. Read More

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December 2021

Dynamic Role of Phospholipases A2 in Health and Diseases in the Central Nervous System.

Cells 2021 10 30;10(11). Epub 2021 Oct 30.

Department of Biomedical Engineering, University of Illinois at Chicago, Chicago, IL 60607, USA.

Phospholipids are major components in the lipid bilayer of cell membranes. These molecules are comprised of two acyl or alkyl groups and different phospho-base groups linked to the glycerol backbone. Over the years, substantial interest has focused on metabolism of phospholipids by phospholipases and the role of their metabolic products in mediating cell functions. Read More

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October 2021

Disulfide Dimerization of Neuronal Calcium Sensor-1: Implications for Zinc and Redox Signaling.

Int J Mol Sci 2021 Nov 22;22(22). Epub 2021 Nov 22.

Belozersky Institute of Physico Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia.

Neuronal calcium sensor-1 (NCS-1) is a four-EF-hand ubiquitous signaling protein modulating neuronal function and survival, which participates in neurodegeneration and carcinogenesis. NCS-1 recognizes specific sites on cellular membranes and regulates numerous targets, including G-protein coupled receptors and their kinases (GRKs). Here, with the use of cellular models and various biophysical and computational techniques, we demonstrate that NCS-1 is a redox-sensitive protein, which responds to oxidizing conditions by the formation of disulfide dimer (dNCS-1), involving its single, highly conservative cysteine C38. Read More

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November 2021

Coenzyme Q at the Hinge of Health and Metabolic Diseases.

Antioxidants (Basel) 2021 Nov 8;10(11). Epub 2021 Nov 8.

Centro Andaluz de Biología del Desarrollo, Instituto de Salud Carlos III, Universidad Pablo de Olavide-CSIC-JA, and CIBERER, 41013 Sevilla, Spain.

Coenzyme Q is a unique lipidic molecule highly conserved in evolution and essential to maintaining aerobic metabolism. It is endogenously synthesized in all cells by a very complex pathway involving a group of nuclear genes that share high homology among species. This pathway is tightly regulated at transcription and translation, but also by environment and energy requirements. Read More

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November 2021

Long-chain fatty acid oxidation and respiratory complex I deficiencies distinguish Barth Syndrome from idiopathic pediatric cardiomyopathy.

J Inherit Metab Dis 2022 Jan 7;45(1):111-124. Epub 2021 Dec 7.

Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA.

Barth syndrome (BTHS) is an X-linked disorder that results from mutations in the TAFAZZIN gene, which encodes a phospholipid transacylase responsible for generating the mature form of cardiolipin in inner mitochondrial membranes. BTHS patients develop early onset cardiomyopathy and a derangement of intermediary metabolism consistent with mitochondrial disease, but the precise alterations in cardiac metabolism that distinguish BTHS from idiopathic forms of cardiomyopathy are unknown. We performed the first metabolic analysis of myocardial tissue from BTHS cardiomyopathy patients compared to age- and sex-matched patients with idiopathic dilated cardiomyopathy (DCM) and nonfailing controls. Read More

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January 2022

( -)-Epicatechin and cardiometabolic risk factors: a focus on potential mechanisms of action.

Pflugers Arch 2022 Jan 23;474(1):99-115. Epub 2021 Nov 23.

Facultad de Farmacia y Bioquímica, Departamento de Ciencias Químicas, Cátedra de Fisicoquímica, Universidad de Buenos Aires, Junín 956, 1113, Buenos Aires, Argentina.

This review summarizes experimental evidence on the beneficial effects of ( -)-epicatechin (EC) attenuating major cardiometabolic risk factors, i.e., dyslipidemias, obesity (adipose tissue dysfunction), hyperglycemia (insulin resistance), and hypertension (endothelial dysfunction). Read More

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January 2022

SARS-CoV-2 deregulates the vascular and immune functions of brain pericytes via Spike protein.

Neurobiol Dis 2021 12 13;161:105561. Epub 2021 Nov 13.

Neuroscience Axis, Research Center of CHU de Québec - Université Laval, Quebec City, QC, Canada; Department of Psychiatry and Neuroscience, Faculty of Medicine, Université Laval, Quebec City, QC, Canada. Electronic address:

Coronavirus disease 19 (COVID-19) is a respiratory illness caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). COVID-19 pathogenesis causes vascular-mediated neurological disorders via elusive mechanisms. SARS-CoV-2 infects host cells via the binding of viral Spike (S) protein to transmembrane receptor, angiotensin-converting enzyme 2 (ACE2). Read More

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December 2021

Comparison of photodynamic efficiency of cholesterol, selected cholesterol esters, metabolites and oxidation products on lipid peroxidation processes.

Acta Biochim Pol 2021 Nov;68(4):527-533

Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.

Cholesterol (Ch) is one of the most important components of biological membranes, which has a significant impact on their biophysical properties. As a key component of lipid membranes, Ch along with other unsaturated lipids present in a biological membrane undergoes oxidation reaction during oxidative stress. Cholesterol oxidation products, cholesteryl esters and metabolites are also localise in lipid membranes, where they may modify membrane properties. Read More

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November 2021

Balancing of mitochondrial translation through METTL8-mediated mC modification of mitochondrial tRNAs.

Mol Cell 2021 12 12;81(23):4810-4825.e12. Epub 2021 Nov 12.

Regensburg Center for Biochemistry (RCB), Laboratory for RNA Biology, University of Regensburg, 93053 Regensburg, Germany. Electronic address:

Mitochondria contain a specific translation machinery for the synthesis of mitochondria-encoded respiratory chain components. Mitochondrial tRNAs (mt-tRNAs) are also generated from the mitochondrial DNA and, similar to their cytoplasmic counterparts, are post-transcriptionally modified. Here, we find that the RNA methyltransferase METTL8 is a mitochondrial protein that facilitates 3-methyl-cytidine (mC) methylation at position C of the mt-tRNA and mt-tRNA. Read More

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December 2021

Antioxidant Properties of Hydrogen Gas Attenuates Oxidative Stress in Airway Epithelial Cells.

Molecules 2021 Oct 21;26(21). Epub 2021 Oct 21.

Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University, Wonju, Gangwon-do, Chuncheon 26426, Korea.

Oxidative stress plays a crucial role in the development of airway diseases. Recently, hydrogen (H) gas has been explored for its antioxidant properties. This study investigated the role of H gas in oxidative stress-induced alveolar and bronchial airway injury, where A549 and NCI-H292 cells were stimulated with hydrogen peroxide (HO) and lipopolysaccharide (LPS) in vitro. Read More

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October 2021

Simple Does Not Mean Trivial: Behavior of Phosphatidic Acid in Lipid Mono- and Bilayers.

Int J Mol Sci 2021 Oct 26;22(21). Epub 2021 Oct 26.

Laboratory of Cytobiochemistry, Faculty of Biotechnology, University of Wroclaw, F. Joliot-Curie 14a, 50-383 Wroclaw, Poland.

Phosphatidic acid (PA) is one of the simplest membrane phospholipids, yet it plays a crucial role in various biologically relevant processes that take place in cells. Since PA generation may be triggered by a variety of factors, very often of antagonistic character, the specific nature of physiological responses driven by PA is not clear. In order to shed more light on these issues, we carried out a systematic characterization of membranes containing one of the three biologically significant PA molecular species. Read More

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October 2021

Lipid metabolism in cancer progression and therapeutic strategies.

MedComm (2020) 2021 Mar 24;2(1):27-59. Epub 2020 Dec 24.

Department of General Surgery, Huashan Hospital & Cancer Metastasis Institute & Institutes of Biomedical Sciences Fudan University Shanghai China.

Dysregulated lipid metabolism represents an important metabolic alteration in cancer. Fatty acids, cholesterol, and phospholipid are the three most prevalent lipids that act as energy producers, signaling molecules, and source material for the biogenesis of cell membranes. The enhanced synthesis, storage, and uptake of lipids contribute to cancer progression. Read More

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Synaptic Zn potentiates the effects of cocaine on striatal dopamine neurotransmission and behavior.

Transl Psychiatry 2021 Nov 8;11(1):570. Epub 2021 Nov 8.

Biobehavioral Imaging and Molecular Neuropsychopharmacology Unit, National Institute on Drug Abuse Intramural Research Program, Baltimore, MD, 21224, USA.

Cocaine binds to the dopamine (DA) transporter (DAT) to regulate cocaine reward and seeking behavior. Zinc (Zn) also binds to the DAT, but the in vivo relevance of this interaction is unknown. We found that Zn concentrations in postmortem brain (caudate) tissue from humans who died of cocaine overdose were significantly lower than in control subjects. Read More

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November 2021

Does silicon really matter for the photosynthetic machinery in plants…?

Plant Physiol Biochem 2021 Dec 4;169:40-48. Epub 2021 Nov 4.

Department of Plant Physiology, Slovak University of Agriculture, A. Hlinku 2, 94976, Nitra, Slovak Republic; Department of Botany and Plant Physiology, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 16500, Prague, Czech Republic. Electronic address:

Silicon (Si) is known to alleviate the adverse impact of different abiotic and biotic stresses by different mechanisms including morphological, physiological, and genetic changes. Photosynthesis, one of the most important physiological processes in the plant is sensitive to different stress factors. Several studies have shown that Si ameliorates the stress effects on photosynthesis by protecting photosynthetic machinery and its function. Read More

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December 2021

Bicontinuous inverted cubic phase stabilization as an index of antimicrobial and membrane fusion peptide activity.

Authors:
D P Siegel

Biochim Biophys Acta Biomembr 2022 02 5;1864(1):183815. Epub 2021 Nov 5.

Givaudan Inc., 1199 Edison Drive, Cincinnati, OH 45216, United States of America. Electronic address:

Some antimicrobial peptides (AMPs) and membrane fusion-catalyzing peptides (FPs) stabilize bicontinuous inverted cubic (Q) phases. Previous authors proposed a topological rationale: since AMP-induced pores, fusion intermediates, and Q phases all have negative Gaussian curvature (NGC), peptides which produce NGC in one structure also do it in another. This assumes that peptides change the curvature energy of the lipid membranes. Read More

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February 2022