1,946 results match your criteria biochip applications

Assessment of the ANDE 6C Rapid DNA system and investigative biochip for the processing of calcified and muscle tissue.

Forensic Sci Int Genet 2021 May 7;53:102526. Epub 2021 May 7.

DNA Support Unit, Federal Bureau of Investigation Laboratory, 2501 Investigation Parkway, Quantico, VA 22135, USA. Electronic address:

The ANDE 6C Rapid DNA system could offer a potential alternative for the processing of calcified and soft tissue samples, often encountered in mass disaster scenarios. While originally designed for single source buccal swabs, interest in the performance of these instruments when using other types of single source samples continues to grow. To enhance the recovery of otherwise lesser quality samples, the manufacturer developed the investigative biochip, an alternative to the NDIS approved Arrestee biochip for reference sample buccal swabs. Read More

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An Oligonucleotide-Distortion-Responsive Organic Transistor for Platinum-Drug-Induced DNA-Damage Detection.

Adv Mater 2021 May 13:e2100489. Epub 2021 May 13.

Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.

Organic transistor with DNA-damage evaluation ability can open up novel opportunities for bioelectronic devices. Even though trace amounts of drugs can cause cumulative gene damage in vivo, the extremely low occurrence proportion makes them hardly transduced into detectable electric signals. Here, an ultrasensitive DNA-damage sensor based on an oligonucleotide-distortion-responsive organic transistor (DROT) is reported by creating controllable conformation change of double-stranded DNA on the surface of organic semiconductors. Read More

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A simple lectin-based biochip might display the potential clinical value of glycomics in patients with spontaneous intracerebral hemorrhage.

Ann Transl Med 2021 Apr;9(7):544

Department of Pathology, Anhui Medical University, Hefei, China.

Background: Intracerebral hemorrhage (ICH) is a cerebrovascular disease with extremely high disability and mortality rates. Glycans play critical roles in biological processes. However, whether glycans can serve as potential biomarkers for determining clinical diagnosis and prognosis in ICH remains determined. Read More

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A nano-integrated microfluidic biochip for enzyme-based point-of-care detection of creatinine.

Chem Commun (Camb) 2021 May;57(38):4726-4729

Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China. and University of Chinese Academy of Sciences, Beijing 100049, P. R. China and Institute of Molecular Medicine, Renji Hospital, School of Medicine and School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Shanghai Jiao Tong University, Shanghai 200127, P. R. China.

A nano-integrated portable enzymatic microfluidic electrochemical biochip was developed for single-step point-of-care testing of creatinine. The biochip could automatically eliminate a lot of interferences from practical biological samples and enzymatic intermediate products. Gold nanostructure- and carbon nanotube-based screen-printed carbon electrodes were integrated into microfluidic structures to improve the detection performance for creatinine. Read More

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Upregulation of Inflammatory Cytokines in Pulmonary Embolism Using Biochip-Array Profiling.

Clin Appl Thromb Hemost 2021 Jan-Dec;27:10760296211013107

Division of Cardiology, Department of Medicine, Loyola University Medical Center, Stritch School of Medicine, Maywood, IL, USA.

The complex pathophysiology of pulmonary embolism (PE) involves hemostatic activation, inflammatory processes, cellular dysfunction, and hemodynamic derangements. Due to the heterogeneity of this disease, risk stratification and diagnosis remains challenging. Biochip-array technology provides an integrated high throughput method for analyzing blood plasma samples for the simultaneous measurement of multiple biomarkers for potential risk stratification. Read More

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NEM-Tar: A Probabilistic Graphical Model for Cancer Regulatory Network Inference and Prioritization of Potential Therapeutic Targets From Multi-Omics Data.

Front Genet 2021 22;12:608042. Epub 2021 Apr 22.

Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China.

Targeted therapy has been widely adopted as an effective treatment strategy to battle against cancer. However, cancers are not single disease entities, but comprising multiple molecularly distinct subtypes, and the heterogeneity nature prevents precise selection of patients for optimized therapy. Dissecting cancer subtype-specific signaling pathways is crucial to pinpointing dysregulated genes for the prioritization of novel therapeutic targets. Read More

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Real-time PCR biochip for on-site detection of Coxiella burnetii in ticks.

Parasit Vectors 2021 May 6;14(1):239. Epub 2021 May 6.

Parasitic and Honeybee Disease Laboratory, Bacterial and Parasitic Disease Division, Department of Animal & Plant Health Research, Animal and Plant Quarantine Agency, Gimcheon, 39660, Republic of Korea.

Background: Q fever, a zoonosis caused by Coxiella burnetii, has adverse effects on public health. Ticks are vectors of C. burnetii and they contribute to the transmission of the pathogen. Read More

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Exposure of ovalbumin during pregnancy prevents the development of allergic rhinitis in offspring through the induction of mast cell autophagy.

Cell Immunol 2021 Apr 17;365:104362. Epub 2021 Apr 17.

Biochip Research Center, Hoseo University, Asan, Chungnam 31499, Republic of Korea; Department of Food Science & Technology, Hoseo University, Asan 31499, Republic of Korea. Electronic address:

Most allergic disease studies have focused on postnatal chemical or microbial exposure. Recent studies have indicated that allergic diseases are associated with the immunological interaction between the mother and her offspring, but the relevant mechanisms are unclear. The aim of this study was to assess whether maternal exposure to allergens during pregnancy could affect allergic rhinitis (AR) in the offspring. Read More

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Homoharringtonine inhibited breast cancer cells growth via miR-18a-3p/AKT/mTOR signaling pathway.

Int J Biol Sci 2021 2;17(4):995-1009. Epub 2021 Mar 2.

The General Hospital of Ningxia Medical University, Biochip Research Center, Yinchuan, 750001, China.

Homoharringtonine (HHT), a natural alkaloid derived from the , exhibited its anti-cancer effects in hematological malignancies clinically. However, its pesticide effects and mechanisms in treating solid tumors remain unclear. In this study, we found that HHT was capable of inhibiting tumor growth after 5-days treatment of breast cancer cells, MCF-7, . Read More

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Gene expression profiling of early Parkinson's disease patient reveals redox homeostasis.

Neurosci Lett 2021 05 12;753:135893. Epub 2021 Apr 12.

Neurotoxin Research Center of Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Neurological Department of Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, PR China. Electronic address:

Parkinson's disease (PD) is slowly progressive. Due to the lack of specific and sensitive biomarkers, the majority of PD patients are in the advanced stages when diagnosed. This study aimed to investigate biomarkers for early PD diagnosis. Read More

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Defining super-enhancer landscape in triple-negative breast cancer by multiomic profiling.

Nat Commun 2021 04 14;12(1):2242. Epub 2021 Apr 14.

Department of Biomedical Sciences, City University of Hong Kong, Kowloon, Hong Kong.

Breast cancer is a heterogeneous disease, affecting over 3.5 million women worldwide, yet the functional role of cis-regulatory elements including super-enhancers in different breast cancer subtypes remains poorly characterized. Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer with a poor prognosis. Read More

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Lithographic Processes for the Scalable Fabrication of Micro- and Nanostructures for Biochips and Biosensors.

ACS Sens 2021 Apr 8. Epub 2021 Apr 8.

Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, United Kingdom.

Since the early 2000s, extensive research has been performed to address numerous challenges in biochip and biosensor fabrication in order to use them for various biomedical applications. These biochips and biosensor devices either integrate biological elements (e.g. Read More

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Quantitative detection of circulating MT-ND1 as a potential biomarker for colorectal cancer.

Bosn J Basic Med Sci 2021 Mar 15. Epub 2021 Mar 15.

Shanghai National Engineering Research Center for Biochip at Shanghai and Shanghai Biochip Limited Corporation, Shanghai, China.

Liquid biopsy represents a diagnostic and monitoring tool and the circulating cell-free mitochondrial DNA (mtDNA) plays a vital role in tumor diagnosis and dynamic assessment. Colorectal cancer (CRC) is one of the most common fatal cancers worldwide. Mitochondrially encoded NADH dehydrogenase subunit 1 (MT-ND1) encodes the biggest subunit of respiratory complex I of mtDNA, and mutations in the MT-ND1 are common in CRC. Read More

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Clinical and etiological analysis of co-infections and secondary infections in COVID-19 patients: An observational study.

Clin Respir J 2021 Apr 5. Epub 2021 Apr 5.

Department of Clinical Laboratory, Shenzhen Third People's Hospital, Southern University of Science and Technology, National Clinical Research Center for Infectious Diseases, Shenzhen, China.

Background: Co-infections, secondary bacterial or fungal infections, are important risk factors for poor outcomes in viral infections. The prevalence of co-infection and secondary infection in patients infected with SARS-CoV-2 is not well understood.

Aims: To investigate the role of co-infections and secondary infections in disease severity of hospitalized individuals with COVID-19. Read More

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Microscopic Analysis of Bacterial Inoculum Effect Using Micropatterned Biochip.

Antibiotics (Basel) 2021 Mar 13;10(3). Epub 2021 Mar 13.

School of Mechanical Engineering, Kookmin University, 77 Jeongneung-ro, Seongbuk-gu, Seoul 02707, Korea.

Antimicrobial resistance has become a major problem in public health and clinical environments. Against this background, antibiotic susceptibility testing (AST) has become necessary to cure diseases in an appropriate and timely manner as it indicates the necessary concentration of antibiotics. Recently, microfluidic based rapid AST methods using microscopic analysis have been shown to reduce the time needed for the determination of the proper antibiotics. Read More

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Detecting Bacterial Cell Viability in Few µL Solutions from Impedance Measurements on Silicon-Based Biochips.

Int J Mol Sci 2021 Mar 29;22(7). Epub 2021 Mar 29.

Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745 Jena, Germany.

Using two different types of impedance biochips (PS5 and BS5) with ring top electrodes, a distinct change of measured impedance has been detected after adding 1-5 µL (with dead or live Gram-positive JG-A12 cells to 20 µL DI water inside the ring top electrode. We relate observed change of measured impedance to change of membrane potential of JG-A12 cells. In contrast to impedance measurements, optical density (OD) measurements cannot be used to distinguish between dead and live cells. Read More

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Low-Cost and Scalable Platform with Multiplexed Microwell Array Biochip for Rapid Diagnosis of COVID-19.

Research (Wash D C) 2021 12;2021:2813643. Epub 2021 Mar 12.

Department of Laboratory Medicine, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu 610041, China.

Sensitive detection of SARS-CoV-2 is of great importance for inhibiting the current pandemic of COVID-19. Here, we report a simple yet efficient platform integrating a portable and low-cost custom-made detector and a novel microwell array biochip for rapid and accurate detection of SARS-CoV-2. The instrument exhibits expedited amplification speed that enables colorimetric read-out within 25 minutes. Read More

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A Highly Automated Mobile Laboratory for On-site Molecular Diagnostics in the COVID-19 Pandemic.

Clin Chem 2021 03;67(4):672-683

Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China.

Background: Infectious disease outbreaks such as the COVID-19 (coronavirus disease 2019) pandemic call for rapid response and complete screening of the suspected community population to identify potential carriers of pathogens. Central laboratories rely on time-consuming sample collection methods that are rarely available in resource-limited settings.

Methods: We present a highly automated and fully integrated mobile laboratory for fast deployment in response to infectious disease outbreaks. Read More

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Biochip-based approach for comprehensive pharmacogenetic testing.

Drug Metab Pers Ther 2020 Dec 14. Epub 2020 Dec 14.

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.

Objectives: Individual sensitivity to many widely used drugs is significantly associated with genetic factors. The purpose of our work was to develop an instrument for simultaneous determination of the most clinically relevant pharmacogenetic markers to allow personalized treatment, mainly in patients with cardiovascular diseases.

Methods: Multiplex one-step polymerase chain reaction (PCR) followed by hybridization on a low-density biochip was applied to interrogate 15 polymorphisms in the following eight genes:  -1639 G>A 1297 G>A 2374 C>G *2,*3 (430 C>T, 1075 A>C) (2549delA, 1846 G>A, 1707delT, 2615_2617delAAG, 2988 G>A), (681 G>A, 636 G>A, -806 C>T) (3435 C>T). Read More

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December 2020

Standard operation procedure for switchSENSE DRX systems.

Eur Biophys J 2021 Mar 27. Epub 2021 Mar 27.

Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198, Gif-sur-Yvette, France.

There is currently a large panel of technologies available to address molecular interactions in vitro. Each technology presents individual advantages and drawbacks, and it becomes challenging to choose which technology will be best suited for a molecular interaction of interest. Approaches can be broadly categorized as either microfluidic surface-bound methods (such as Surface Plasmon Resonance (SPR) or switchSENSE) or in-solution methods (such as Isothermal Titration Calorimetry (ITC) or MicroScale Thermophoresis (MST)). Read More

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Point-of-Care Pathogen Testing Using Photonic Crystals and Machine Vision for Diagnosis of Urinary Tract Infections.

Nano Lett 2021 Apr 26;21(7):2854-2860. Epub 2021 Mar 26.

Key Laboratory of Biomedical Polymers of Ministry of Education, College of Chemistry and Molecular Sciences, School of Microelectronics, Wuhan University, Wuhan 430072, China.

Urinary tract infections (UTIs) caused by bacterial invasion can lead to life-threatening complications, posing a significant health threat to more than 150 million people worldwide. As a result, there is need for accurate and rapid diagnosis of UTIs to enable more effective treatment. Described here is an intelligent diagnostic system constructed for bacterial detection using an immunobiosensor, signal-amplification biochip, and image processing algorithm based on machine vision. Read More

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Comparative Analysis of BIOCHIP Mosaic-based Indirect Immunofluorescence with Direct Immunofluorescence in Diagnosis of Autoimmune Bullous Diseases: A Cross-Sectional Study.

Indian Dermatol Online J 2021 Jan-Feb;12(1):105-109. Epub 2021 Jan 16.

Department of Pathology, PSG Institute of Medical Sciences and Research, Coimbatore, Tamil Nadu, India.

Background: Autoimmune bullous diseases (AIBD) are a heterogeneous group of diseases characterized by autoantibodies against desmosomal proteins in the pemphigus group of disorders and adhesion molecules of the dermal-epidermal junction in pemphigoid group of diseases. Direct immunofluorescence (DIF) establishes the diagnosis of AIBD by demonstrating intercellular deposits of IgG and C3 in case of pemphigus and linear deposits of IgG and C3 along the basement membrane zone (BMZ) in bullous pemphigoid (BP). BIOCHIP mosaic-based indirect immunofluorescence (IIF), a novel diagnostic approach employs detection of characteristic staining pattern and target antigens in a single miniature incubation field. Read More

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January 2021

An integrated workflow for biomarker development using microRNAs in extracellular vesicles for cancer precision medicine.

Semin Cancer Biol 2021 Mar 22. Epub 2021 Mar 22.

Department of Biomedical Sciences, City University of Hong Kong, 31 To Yuen Street, Kowloon Tong, Hong Kong; Tung Biomedical Sciences Centre, City University of Hong Kong, Hong Kong; Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute, City University of Hong Kong, Shenzhen, Guangdong Province, China. Electronic address:

EV-miRNAs are microRNA (miRNA) molecules encapsulated in extracellular vesicles (EVs), which play crucial roles in tumor pathogenesis, progression, and metastasis. Recent studies about EV-miRNAs have gained novel insights into cancer biology and have demonstrated a great potential to develop novel liquid biopsy assays for various applications. Notably, compared to conventional liquid biomarkers, EV-miRNAs are more advantageous in representing host-cell molecular architecture and exhibiting higher stability and specificity. Read More

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Platelet-rich plasma for androgenic alopecia: A randomized, placebo-controlled, double-blind study and combined mice model experiment.

J Cosmet Dermatol 2021 Mar 22. Epub 2021 Mar 22.

Department of Plastic and Aesthetic Surgery, Nanfang Hospital of Southern Medical University, Guangzhou, China.

Background: Platelet rich plasma (PRP) has been accepted as a potential therapy for treating androgenetic alopecia (AGA).

Objective: To fully clarify the underling molecular mechanisms of PRP action on hair growth and promote its clinical applications.

Methods: In this study, we used mice models and protein biochip to explore the specific mechanisms of PRP regulating hair growth. Read More

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Cleavage and Polyadenylation Specific Factor 1 Promotes Tumor Progression Alternative Polyadenylation and Splicing in Hepatocellular Carcinoma.

Front Cell Dev Biol 2021 4;9:616835. Epub 2021 Mar 4.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.

Alternative polyadenylation (APA) is an important post-transcriptional regulatory mechanism required for cleavage and polyadenylation (CPA) of the 3' untranslated region (3' UTR) of mRNAs. Several aberrant APA events have been reported in hepatocellular carcinoma (HCC). However, the regulatory mechanisms underlying APA remain unclear. Read More

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Carboxypeptidase A4 negatively correlates with p53 expression and regulates the stemness of breast cancer cells.

Int J Med Sci 2021 18;18(8):1753-1759. Epub 2021 Feb 18.

State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, P.R. China.

Triple-negative breast cancer (TNBC) is an aggressive cancer subtype lacking effective treatment options, and p53 is the most frequently mutated or deleted gene. Carboxypeptidase A4 (CPA4) is an extracellular metallocarboxypeptidase, which was closely associated with aggressiveness. Although a recent study indicated that CPA4 could induce epithelial‑mesenchymal transition in breast cancer cells, no studies investigated its stemness-related function and the correlation between CPA4 and p53 in TNBC. Read More

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February 2021

A wideband picosecond pulsed electric fields (psPEF) exposure system for the nanoporation of biological cells.

Bioelectrochemistry 2021 Feb 26;140:107790. Epub 2021 Feb 26.

Department of Biological Science and Bioengineering, Key Laboratory of Biomedical Information Engineering of the Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.

The effects and mechanisms of ultrashort and intense pulsed electric fields on biological cells remain some unknown. Especially for picosecond pulsed electric fields (psPEF) with a high pulse repetition rate, electroporation or nanoporation effects could be induced on cell membranes and intracellular organelle membranes. In this work, the design, implementation, and experimental validation of a wideband psPEF exposure system (WPES) is reported, comprising picosecond pulser and wideband biochip, for the in vitro exposure of suspended cells to high-intensity psPEF. Read More

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February 2021

Attomolar-Level Ultrasensitive and Multiplex microRNA Detection Enabled by a Nanomaterial Locally Assembled Microfluidic Biochip for Cancer Diagnosis.

Anal Chem 2021 03 15;93(12):5129-5136. Epub 2021 Mar 15.

Institute of Marine Science and Technology, Shandong University, Qingdao, Shandong 266237, China.

Non-invasive early diagnosis is of great significance in disease pathologic development and subsequent medical treatments, and microRNA (miRNA) detection has attracted critical attention in early cancer screening and diagnosis. High-throughput, sensitive, economic, and fast miRNA sensing platforms are necessary to realize the low-concentration miRNA detection in clinical diagnosis and biological studies. Here, we developed an attomolar-level ultrasensitive, rapid, and multiple-miRNA simultaneous detection platform enabled by nanomaterial locally assembled microfluidic biochips. Read More

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Single cell capture, isolation, and long-term in-situ imaging using quantitative self-interference spectroscopy.

Cytometry A 2021 Mar 11. Epub 2021 Mar 11.

Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China.

Single cell research with microfluidic chip is of vital importance in biomedical studies and clinical medicine. Simultaneous microfluidic cell manipulations and long-term cell monitoring needs further investigations due to the lack of label-free quantitative imaging techniques and systems. In this work, single cell capture, isolation and long-term in-situ monitoring was realized with a microfluidic cell chip, compact cell incubator and quantitative self-interference spectroscopy. Read More

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Diagnosis of Epidermolysis Bullosa Acquisita: Multicentre Comparison of Different Assays for Serum Anti-type VII Collagen Reactivity.

Acta Derm Venereol 2021 Mar 23;101(3):adv00420. Epub 2021 Mar 23.

Department of Dermatology, University of Lubeck, DE-23522 Lubeck, Germany.

Epidermolysis bullosa acquisita is a pemphigoid disease characterized by autoantibodies against type VII collagen. This study compared the sensitivity and specificity of 6 diagnostic assays: type VII collagen non-collagenous domains enzyme-linked immunoassay (NC1/2 ELISA) (MBL, Nagoya, Japan); type VII collagen NC1 ELISA (Euroimmun, Lübeck, Germany); indirect immunofluorescence (IF) microscopy test based on the expression of recombinant NC1 in a human cell line (NC1 BIOCHIP®; Euroimmun); full-length recombinant type VII collagen ELISA; immunoblotting with full-length type VII collagen in the extract of human dermis; and immunoblotting with recombinant NC1. Immunoblotting with recombinant NC1 showed a sensitivity of 93. Read More

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