1,551,265 results match your criteria binding

A kinetic view of clathrin assembly and endocytic cargo sorting.

Min Wu Xudong Wu

Curr Opin Cell Biol 2021 Apr 13;71:130-138. Epub 2021 Apr 13.

Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA, 02115, USA.

Specificity and sensitivity in biochemical reactions can be achieved through regulation of equilibrium binding affinity or through proofreading mechanisms that allow for the dissociation of unwanted intermediates. In this essay, we aim to provide our perspectives on how the concept of kinetic proofreading might apply in the context of cargo sorting in clathrin-mediated endocytosis. Read More

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Osteocalcin promotes proliferation, differentiation, and survival of PC12 cells.

Biochem Biophys Res Commun 2021 Apr 14;557:174-179. Epub 2021 Apr 14.

Division of Applied Pharmacology, Department of Health Promotion, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu 803-8580, Japan. Electronic address:

Involvement of the bone matrix protein osteocalcin (OC) in the development of learning and memory, and the prevention of anxiety-like behaviors in mice. However, the direct effects of OC on neurons are still unknown comparing to the mechanism how OC affects systemic energy expenditure and glucose homeostasis. In this study, we investigated the effect of OC on proliferation, differentiation, and survival of neurons using the rat pheochromocytoma cell line PC12. Read More

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Design of artificial metalloenzymes for the reduction of nicotinamide cofactors.

J Inorg Biochem 2021 Apr 2;220:111446. Epub 2021 Apr 2.

Sustainable Process Technologies, Faculty of Engineering, University of Nottingham, Nottingham, United Kingdom. Electronic address:

Artificial metalloenzymes result from the insertion of a catalytically active metal complex into a biological scaffold, generally a protein devoid of other catalytic functionalities. As such, their design requires efforts to engineer substrate binding, in addition to accommodating the artificial catalyst. Here we constructed and characterised artificial metalloenzymes using alcohol dehydrogenase as starting point, an enzyme which has both a cofactor and a substrate binding pocket. Read More

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Investigation of protein-ligand complexes by ligand-based NMR methods.

Carbohydr Res 2021 Apr 9;503:108313. Epub 2021 Apr 9.

Dipartimento di Scienze Chimiche, Complesso Universitario Monte Sant'Angelo, Università di Napoli Federico II, Via Cintia 4, I-80126, Napoli, Italy; CNR, Institute for Polymers, Composites and Biomaterials, IPCB ss, Catania, Italy. Electronic address:

Molecular recognition is at the base of all biological events and its knowledge at atomic level is pivotal in the development of new drug design approaches. NMR spectroscopy is one of the most widely used technique to detect and characterize transient ligand-receptor interactions in solution. In particular, ligand-based NMR approaches, including NOE-based NMR techniques, diffusion experiments and relaxation methods, are excellent tools to investigate how ligands interact with their receptors. Read More

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Tumor vasculature-targeting PEGylated peptide-drug conjugate prodrug nanoparticles improve chemotherapy and prevent tumor metastasis.

Eur J Med Chem 2021 Apr 5;219:113430. Epub 2021 Apr 5.

China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, College of Biotechnology Tianjin University of Science and Technology, No.29 of 13 th Avenue, TEDA, Tianjin, 300457, PR China. Electronic address:

Metastasis is the main cause of death in cancer patients; therefore, new strategies or technologies that can inhibit the growth of primary tumors and their metastatic spread are extremely valuable. In this study, we selected an E-selectin-binding peptide as a targeting ligand and an inhibitor of metastasis, and conjugated this peptide with SN38 and PEG to produce an amphiphilic PEGylated peptide-drug conjugate (PDC). Novel self-assembled nanoparticles were then formed by the amphiphilic conjugate. Read More

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Comparative study of HSO/SO versus HPO functionalities anchored on TiO-supported antimony oxide-vanadium oxide-cerium oxide composites for low-temperature NO activation.

J Hazard Mater 2021 Apr 6;416:125780. Epub 2021 Apr 6.

Extreme Materials Research Center, Korea Institute of Science and Technology, Seoul 02792, South Korea. Electronic address:

TiO-supported antimony oxide-vanadium oxide-cerium oxide (SVC) imparts Lewis acidic (L)/Brönsted acidic (B) sites, labile (O)/mobile oxygens (O), and oxygen vacancies (O) for selective catalytic NO reduction (SCR). However, these species are harmonious occasionally, readily poisoned by HO/sulfur/phosphorus/carbon, thus limiting SCR performance of SVC. Herein, a synthetic means is reported for immobilizing HSO/SO (A= 3-4) or HPO (B= 1-3) on the L sites of SVC to form SVC-S and SVC-P. Read More

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Binding affinity of benzalkonium chloride on contact lens surfaces and the effects on their physical properties.

Colloids Surf B Biointerfaces 2021 Apr 3;203:111744. Epub 2021 Apr 3.

Department of Optometry, College of Energy and Biotechnology, Seoul National University of Science and Technology, Seoul, 01811, Republic of Korea; Convergence Institute of Biomaterials and Bioengineering, Seoul National University of Science and Technology, Seoul, 01811, Republic of Korea. Electronic address:

Benzalkonium chloride (BKC) is a cationic surfactant used as a component in ophthalmic eye drops. The effects of BKC on the eye and the simple binding of BKC on the contact lens surface have been reported in other studies. However, the exact value of the BKC binding affinity on the contact lens surfaces and its effects on the physical properties of contact lenses have not been studied. Read More

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Exploring the quorum sensing inhibition of isolated chrysin from Penicillium chrysogenum DXY-1.

Bioorg Chem 2021 Apr 8;111:104894. Epub 2021 Apr 8.

Engineering Research Center of Industrial Biocatalysis, Fujian Province University, Fujian Provincial Key Laboratory of Advanced Materials Oriented Chemical Engineering, Fujian Provincial Key Laboratory of Polymer Materials, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Chemistry and Materials Science, Fujian Normal University, 32 Shangsan Road, Fuzhou 350007, People's Republic of China. Electronic address:

We recently and for the first time reported that ethyl acetate extracts isolated from Penicillium chrysogenum DXY-1 exhibited anti-quorum sensing (anti-QS) activity. Herein, another active molecule in the extracts was identified as chrysin by NMR and MS. A 20 μg/mL dose of chrysin inhibited violacein production regulated by QS in C. Read More

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Performance of the rapid high-throughput automated electrochemiluminescence immunoassay targeting total antibodies to the SARS-CoV-2 spike protein receptor binding domain in comparison to the neutralization assay.

J Clin Virol 2021 Apr 10;139:104820. Epub 2021 Apr 10.

Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 1000 Ljubljana, Slovenia. Electronic address:

Background: Neutralization tests (NT) are the gold standard for detecting and quantifying anti-SARS-CoV-2 neutralizing antibodies (NAb), but their complexity restricts them to research settings or reference laboratories. Antibodies against S protein receptor binding domain (RBD) have been shown to confer a neutralizing activity against SARS-CoV-2. Assays quantitatively measuring anti-S1-RBD-SARS-CoV-2 antibodies could be of great value for NAb screening of potential donors for convalescent-phase plasma therapy, assessing natural or vaccine-induced immunity, stratifying individuals for vaccine receipt, and documenting vaccine response. Read More

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Neonatal exposure to monosodium glutamate results in impaired auditory brainstem structure and function.

Hear Res 2021 Apr 8;405:108243. Epub 2021 Apr 8.

Department of Anatomy, Lake Erie College of Osteopathic Medicine, 1858 West Grandview Blvd, Erie, PA 16504, United States. Electronic address:

Excitotoxic injury during the neonatal period has been shown to result in neurodegenerative changes in several different brain regions. Exposure to monosodium glutamate (MSG) during the first two postnatal weeks results in glutamate neurotoxicity in the cochlea and has been shown to result in damage to cochlear hair cells and fewer neurons in the spiral ganglion. Further, we have shown that such exposure results in fewer neurons in the cochlear nucleus and superior olivary complex and abnormal expression of the calcium binding proteins calbindin and calretinin. Read More

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High-mobility group box 1 serves as an inflammation driver of cardiovascular disease.

Biomed Pharmacother 2021 Apr 14;139:111555. Epub 2021 Apr 14.

Department of Cardiology, the Third Xiangya Hospital of Central South University, Changsha, Hunan, China. Electronic address:

Cardiovascular disease (CVD) is the most deadly disease, which can cause sudden death, in which inflammation is a key factor in its occurrence and development. High-mobility group box 1 (HMGB1) is a novel nuclear DNA-binding protein that activates innate immunity to induce inflammation in CVD. HMGB1 exists in the cytoplasm and nucleus of different cell types, including those in the heart. Read More

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Self-binding directives under the new Dutch Law on Compulsory Mental Health Care: An analysis of the legal framework and a proposal for reform.

Int J Law Psychiatry 2021 Apr 14;76:101699. Epub 2021 Apr 14.

Department of Ethics, Law and Humanities, Amsterdam University Medical Centers, VU University Amsterdam, Netherlands.

Self-binding directives (SBDs) are a special type of psychiatric advance directive by means of which mental health service users can give advance consent to compulsory hospital admission or treatment during a future mental health crisis. SBDs are legally binding in the Netherlands since 2008. On the 1st of January 2020, the Dutch Law on Special Admissions to Psychiatric Hospitals (Wet bijzondere opnemingen in psychiatrische ziekenhuizen; Bopz) was replaced by the new Law on Compulsory Mental Health Care (Wet verplichte geestelijke gezondheidszorg; Wvggz). Read More

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Role of IGFBP-2 in oral cancer metastasis.

Biochim Biophys Acta Mol Basis Dis 2021 Apr 14:166143. Epub 2021 Apr 14.

Department of Medical Science, Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, Taiwan. Electronic address:

Cancer metastasis is one of most main causes of failure in cancer treatment. Nonetheless, more than half of oral cancer patients were diagnosed as advanced oral cancer with dramatically decreased 5-year survival rate to lower than 20%, while the stages become more advanced. In order to improve oral cancer treatment, the identification of cancer metastatic biomarkers and mechanisms is critical. Read More

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Distinctive functioning of STARD1 in the fetal Leydig cells compared to adult Leydig and adrenal cells. Impact of Hedgehog signaling via the primary cilium.

Mol Cell Endocrinol 2021 Apr 14:111265. Epub 2021 Apr 14.

Department of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705. Electronic address:

STARD1 stimulates cholesterol transfer to mitochondrial CYP11A1 for conversion to pregnenolone. A cholesterol-binding START domain is guided by an N-terminal domain in a cell selective manner. Fetal and adult Leydig cells (FLC, ALC) show distinct Stard1 regulation. Read More

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Structural and functional analysis of the simultaneous binding of two duplex/quadruplex aptamers to human α-thrombin.

Int J Biol Macromol 2021 Apr 14. Epub 2021 Apr 14.

Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario di Monte Sant'Angelo, Via Cintia, I-80126 Naples, Italy. Electronic address:

The long-range communication between the two exosites of human α-thrombin (thrombin) tightly modulates the protein-effector interactions. Duplex/quadruplex aptamers represent an emerging class of very effective binders of thrombin. Among them, NU172 and HD22 aptamers are at the forefront of exosite I and II recognition, respectively. Read More

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A distinct structure of Cas1-Cas2 complex provides insights into the mechanism for the longer spacer acquisition in Pyrococcus furiosus.

Int J Biol Macromol 2021 Apr 14. Epub 2021 Apr 14.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, PR China. Electronic address:

In the adaptation stage of CRISPR-Cas systems, the Cas1-Cas2 integrase captures and integrates new invader-derived spacers into the CRISPR locus, serving as a molecular memory of prior infection. As of yet, the structural information of Cas1-Cas2 complex is available only for two species. Here we present the crystal structure of Cas1-Cas2 complex of Pyrococcus furiosus, which showed a distinct architecture from the known Cas1-Cas2 complexes. Read More

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Anti-inflammatory effects of FS48, the first potassium channel inhibitor from the salivary glands of the flea Xenopsylla cheopis.

J Biol Chem 2021 Apr 14:100670. Epub 2021 Apr 14.

Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. Electronic address:

The voltage-gated potassium (Kv) 1.3 channel plays a crucial role in the immune responsiveness of T-lymphocytes and macrophages, presenting a potential target for treatment of immune- and inflammation related-diseases. FS48, a protein from the rodent flea Xenopsylla cheopis, shares the three disulfide bond feature of scorpion toxins. Read More

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SAGA and SAGA-like SLIK transcriptional coactivators are structurally and biochemically equivalent.

J Biol Chem 2021 Apr 14:100671. Epub 2021 Apr 14.

Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia. Electronic address:

The SAGA-like complex SLIK is a modified version of the Spt-Ada-Gcn5-Acetyltransferase (SAGA) complex. SLIK is formed through C-terminal truncation of the Spt7 SAGA subunit, causing loss of Spt8, one of the subunits that interacts with the TATA-binding protein (TBP). SLIK and SAGA are both coactivators of RNA polymerase II transcription in yeast and both SAGA and SLIK perform chromatin modifications. Read More

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Safety, tolerability, and immunogenicity of the respiratory syncytial virus prefusion F subunit vaccine DS-Cav1: a phase 1, randomised, open-label, dose-escalation clinical trial.

Lancet Respir Med 2021 Apr 14. Epub 2021 Apr 14.

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA. Electronic address:

Background: Multiple active vaccination approaches have proven ineffective in reducing the substantial morbidity and mortality caused by respiratory syncytial virus (RSV) in infants and older adults (aged ≥65 years). A vaccine conferring a substantial and sustainable boost in neutralising activity is required to protect against severe RSV disease. To that end, we evaluated the safety and immunogenicity of DS-Cav1, a prefusion F subunit vaccine. Read More

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Host PDZ-containing proteins targeted by SARS-Cov-2.

FEBS J 2021 Apr 17. Epub 2021 Apr 17.

Institut Pasteur, Unité Récepteurs-Canaux, UMR CNRS 3571, 75015, Paris, France.

Small linear motif targeting protein interacting domains called PDZ have been identified at the C-terminus of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins E, 3a, and N. Using a high-throughput approach of affinity-profiling against the full human PDZome, we identified sixteen human PDZ binders of SARS-CoV-2 proteins E, 3A and N showing significant interactions with dissociation constants values ranging from 3 μM to 82 μM. Six of them (TJP1, PTPN13, HTRA1, PARD3, MLLT4, LNX2) are also recognized by SARS-CoV while three (NHERF1, MAST2, RADIL) are specific to SARS-CoV-2 E protein. Read More

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Pr-favoured variants of the bacteriophytochrome from the plant pathogen Xanthomonas campestris hint on light regulation of virulence-associated mechanisms.

FEBS J 2021 Apr 17. Epub 2021 Apr 17.

Fundación Instituto Leloir, IIBBA-CONICET, Av. Patricias Argentinas 435, (C1405BWE) Ciudad Autónoma de Buenos Aires.

Red/far-red light-sensing bacteriophytochrome photoreceptor (BphP) pathways play key roles in bacterial physiology and ecology. These bilin-binding proteins photoswitch between two states, Pr (red absorbing) and Pfr (far-red absorbing). The isomerization of the chromophore and the downstream structural changes result in the light signal transduction. Read More

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New Phytol 2021 Apr 17. Epub 2021 Apr 17.

College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.

Drought stress seriously limits crop productivity. Although studies have been carried out, it is still largely unknown that how plant responds to drought stress. Here we find that drought treatment can enhance the phosphorylation activity of brassinosteroid-signaling kinase 1 (ZmBSK1) in maize (Zea mays). Read More

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Diversity and complexity of mouse allergens in urine, house dust, and allergen extracts assessed with an immuno-allergomic approach.

Allergy 2021 Apr 17. Epub 2021 Apr 17.

Laboratory of Immunobiochemistry, Division of Bacterial, Food and Drug Administration, Parasitic, and Allergenic Products, Silver Spring, MD, USA.

Background: Mouse allergy is an important cause of indoor asthma and allergic rhinoconjunctivitis. The major mouse allergen, Mus m 1, is a complex of homologous pheromone-binding lipocalinscalled major urinary proteins (MUPs).

Methods: We analyzed the proteome of MUPs in mouse urine, commercial mouse epithelial extracts, and environmental samples using several approaches. Read More

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A transcriptional regulatory module controls lipid accumulation in soybean.

New Phytol 2021 Apr 17. Epub 2021 Apr 17.

State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, INASEED, Chinese Academy of Sciences, Beijing, 100101, China.

Soybean (Glycine max) is one of the most important oilseed crops. However, limited information is known about the regulatory mechanism of oil accumulation in soybean. In this study, a member of tandem CCCH zinc finger (TZF) proteins, GmZF392, from our previous RNA-seq analysis for seed-preferred transcription factors, was found to function as a positive regulator of lipid production. Read More

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Apoplastic lipid barriers regulated by conserved homeobox transcription factors extend seed longevity in multiple plant species.

New Phytol 2021 Apr 17. Epub 2021 Apr 17.

Instituto de Biología Molecular y Celular de Plantas, Universitat Politècnica de València-Consejo Superior de Investigaciones Científicas, Camino de Vera, 46022, Valencia, Spain.

Cutin and suberin are lipid polyesters deposited in specific apoplastic compartments. Their fundamental roles in plant biology include controlling the movement of gases, water and solutes, and conferring pathogen resistance. Both cutin and suberin have been shown to be present in the Arabidopsis seed coat where they regulate seed dormancy and longevity. Read More

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Hsa‑circRNA‑G004213 promotes cisplatin sensitivity by regulating miR‑513b‑5p/PRPF39 in liver cancer.

Mol Med Rep 2021 Jun 13;23(6). Epub 2021 Apr 13.

Department of Gastroenterology, Clinical Medical College and The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan 610000, P.R. China.

In recent years, increasing evidence has confirmed that exosomal circular RNAs (circRNAs) serve a crucial role in the prognostic prediction and diagnosis of liver cancer (LC). The present study compared the expression patterns of exosomal circRNAs during transarterial chemoembolization (TACE). CircRNA sequencing analysis identified 390 differentially expressed circRNAs between the prior TACE and following the first TACE operation groups and 489 differentially expressed circRNAs between the prior to TACE and following the second TACE operation groups. Read More

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Molecular basis for higher affinity of SARS-CoV-2 spike RBD for human ACE2 receptor.

Proteins 2021 Apr 17. Epub 2021 Apr 17.

Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, USA.

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has caused substantially more infections, deaths, and economic disruptions than the 2002-2003 SARS-CoV. The key to understanding SARS-CoV-2's higher infectivity lies partly in its host receptor recognition mechanism. Experiments show that the human ACE2 (angiotensin converting enzyme 2) protein, which serves as the primary receptor for both CoVs, binds to the receptor binding domain (RBD) of CoV-2's spike protein stronger than SARS-CoV's spike RBD. Read More

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Soyasaponin ameliorates obesity and reduces hepatic triacylglycerol accumulation by suppressing lipogenesis in high-fat diet-fed mice.

J Food Sci 2021 Apr 17. Epub 2021 Apr 17.

Laboratory of Nutrition Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan.

Soyasaponins are triterpenoid glycosides found in soybean. We investigated whether soyasaponin ameliorates lipid metabolism and its possible mechanisms. In C57BL/6J mice fed a high-fat diet (HFD), soyasaponin (SAP) was orally administered for 9 weeks. Read More

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High-throughput screening identifies established drugs as SARS-CoV-2 PLpro inhibitors.

Protein Cell 2021 Apr 17. Epub 2021 Apr 17.

Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.

A new coronavirus (SARS-CoV-2) has been identified as the etiologic agent for the COVID-19 outbreak. Currently, effective treatment options remain very limited for this disease; therefore, there is an urgent need to identify new anti-COVID-19 agents. In this study, we screened over 6,000 compounds that included approved drugs, drug candidates in clinical trials, and pharmacologically active compounds to identify leads that target the SARS-CoV-2 papain-like protease (PLpro). Read More

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Drosophila NSD deletion induces developmental anomalies similar to those seen in Sotos syndrome 1 patients.

Genes Genomics 2021 Apr 17. Epub 2021 Apr 17.

Department of Biological Sciences, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea.

Background: Haploinsufficiency of the human nuclear receptor binding suppressor of variegation 3-9, enhancer of zeste, and trithorax (SET) domain 1 (NSD1) gene causes a developmental disorder called Sotos syndrome 1 (SOTOS1), which is associated with overgrowth and macrocephaly. NSD family proteins encoding histone H3 lysine 36 (H3K36) methyltransferases are conserved in many species, and Drosophila has a single NSD homolog gene, NSD.

Objective: To gain insight into the biological functions of NSD1 deficiency in the developmental anomalies seen in SOTOS1 patients using an NSD-deleted Drosophila mutant. Read More

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