1,181,373 results match your criteria beta 2-microglobulin

Relationship between DNA mismatch repair and CRISPR/Cas9-mediated knock-in in the bovine β-casein gene locus.

Anim Biosci 2021 Jun 24. Epub 2021 Jun 24.

Department of Animal Science, Chonnam National University, Gwangju 61186, Korea.

Objective: Efficient gene editing technology is critical for successful knock-in in domestic animals. RAD51 gene plays an important role in strand invasion during homologous recombination (HR) in mammals, and is regulated by CHK1 and CHK2 genes, which are upstream elements of RAD51. In addition, mismatch repair (MMR) system is inextricably linked to HR-related pathways and regulates HR via heteroduplex rejection. Read More

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Suffrutines A and B Inhibit the Expression of Inflammatory Mediators in LPS-Induced RAW264.7 Cells by Suppressing the NF-κB Signaling Pathway.

Planta Med 2021 Jul 22. Epub 2021 Jul 22.

School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China.

is a traditional Chinese medicine that has been commonly used for the treatment of inflammatory ailments, including rheumatism and lumbago. Suffrutines A and suffrutines B are a pair of novel E,E and Z,E isomeric indolizidine alkaloids isolated from the roots of . However, their anti-inflammatory activity has not been reported thus far. Read More

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Reductive soil disinfestation attenuates antibiotic resistance genes in greenhouse vegetable soils.

J Hazard Mater 2021 Jul 13;420:126632. Epub 2021 Jul 13.

School of Ecology and Environment, Northwestern Polytechnical University, 710000 Xi'an, Shaanxi, China.

Reductive soil disinfestation (RSD) is an emerging technique that ameliorates soil degradation, but its effects against antibiotic resistance genes (ARGs) were unclear. Here, we examined soil properties, ARG types and numbers, and ARG profiles, and bacterial community compositions following 4 soil treatments: control; straw addition (SA); water flooding (WF); and RSD, both straw addition and water flooding. The results showed that the numbers of ARG types and subtypes decreased by 10. Read More

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Economizing the lignocellulosic hydrolysis process using heterologously expressed auxiliary enzymes feruloyl esterase D (CE1) and β-xylosidase (GH43) derived from thermophilic fungi Scytalidium thermophilum.

Bioresour Technol 2021 Jul 17;339:125603. Epub 2021 Jul 17.

Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab-143005, India. Electronic address:

Two lignocellulolytic accessory enzymes, feruloyl esterase D (FAED_SCYTH) and β-xylosidase (XYL43B_SCYTH) were cloned and produced in the Pichia pastoris X33 as host. The molecular weight of recombinant enzymes FAED_SCYTH and XYL43B_SCYTH were ~ 31 and 40 kDa, respectively. FAED_SCYTH showed optimal activity at pH 6. Read More

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Efficacy and tolerability of Melatonin vs Triclofos to achieve sleep for pediatric electroencephalography: A single blinded randomized controlled trial.

Eur J Paediatr Neurol 2021 Jul 7;34:14-20. Epub 2021 Jul 7.

Department of Pediatrics, Bharati Vidyapeeth Deemed University Medical College, Pune, India. Electronic address:

Purpose: To compare Melatonin with Triclofos for efficacy (proportion of successful EEG, need of augmentation, sleep onset latency (SOL), yield of discharges, duration of sleep, presence and grade of artifacts) and tolerability (adverse effect profile).

Methods: A randomized trial was performed (block randomization). All children were advised regarding sleep deprivation, EEG technician administered the drug. Read More

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Structural insights in galectin-1-glycan recognition: Relevance of the glycosidic linkage and the N-acetylation pattern of sugar moieties.

Bioorg Med Chem 2021 Jul 14;44:116309. Epub 2021 Jul 14.

Laboratorio de Bioquímica, Departamento de Biociencias, Facultad de Química, UdelaR, Gral. Flores, 2124, 11800 Montevideo, Uruguay. Electronic address:

Galectins, soluble lectins widely expressed intra- and extracellularly in different cell types, play major roles in deciphering the cellular glycocode. Galectin-1 (Gal-1), a prototype member of this family, presents a carbohydrate recognition domain (CRD) with specific affinity for β-galactosides such as N-acetyllactosamine (β-d-Galp-(1 → 4)-d-GlcpNAc), and mediate numerous physiological and pathological processes. In this work, Gal-1 binding affinity for β-(1 → 6) galactosides, including β-d-Galp-(1 → 6)-β-d-GlcpNAc-(1 → 4)-d-GlcpNAc was evaluated, and their performance was compared to that of β-(1 → 4) and β-(1 → 3) galactosides. Read More

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Serum levels of TGF-β1, cytokines, angiogenic, and anti-angiogenic factors in pregnant women who smoke.

J Reprod Immunol 2021 Jul 7;147:103351. Epub 2021 Jul 7.

Department of Pediatrics, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada. Electronic address:

Introduction: Women who smoke during pregnancy have a reduced risk of preeclampsia. The mechanism of this association is poorly understood. Preeclampsia is an anti-angiogenic and inflammatory state. Read More

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Establishing amoxicillin allergy in children through direct graded oral challenge (GOC): evaluating risk factors for positive challenges, safety, and risk of cross reactivity to cephalosporines.

J Allergy Clin Immunol Pract 2021 Jul 19. Epub 2021 Jul 19.

Division of Allergy and Clinical Immunology, Department of Pediatrics, Montreal Children's Hospital, Montreal, Quebec, Canada; Infectious Diseases and Immunity in Global Health Program, The Research Institute of the McGill University Health Centre, McGill University, Montreal, Quebec, Canada.

Background: Data on the diagnostic properties of direct oral challenges without the use of skin tests in children with suspected amoxicillin allergy is sparse.

Objective: Assess the use of direct oral challenges.

Methods: A cohort study was conducted between March 2013 and March 2020, in Montreal and Winnipeg. Read More

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A More Universal Approach to Comprehensive Analysis of ThalaSsemia Alleles (CATSA).

J Mol Diagn 2021 Jul 19. Epub 2021 Jul 19.

Hunan Jiahui Genetics Hospital, Changsha, Hunan 410078, China; Center for Medical Genetics and Hunan Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha, Hunan 410078, China. Electronic address:

The aim of the study was to assess the clinical utility of a third generation sequencing (TGS) approach termed Comprehensive Analysis of ThalaSsemia Alleles (CATSA) for identifying both alpha and beta thalassemia genetic carrier status. Prospective blood samples (n = 1759) with abnormal hemoglobin parameters were screened for pathogenic thalassemia variants by CATSA on the PacBio TGS platform. In 1159 individuals, a total of 1317 pathogenic thalassemia variants were identified and confirmed by independent PCR based tests. Read More

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Molecular and epidemiological investigation of a colistin-resistant OXA-23/NDM-1-producing Acinetobacter baumannii outbreak in Southwest Indian Ocean.

Int J Antimicrob Agents 2021 Jul 19:106402. Epub 2021 Jul 19.

Centre National de Référence de la Résistance aux Antibiotiques, Centre Hospitalier Universitaire de Besançon, Besançon, France.; Laboratoire de Bactériologie, UMR CNRS 6249 Chrono-Environnement, Faculté de Médecine-Pharmacie, Université Bourgogne Franche-Comté, Besançon, France.. Electronic address:

Dual resistance to colistin and carbapenems is a milestone reached by certain extensively-drug resistant (XDR) Gram-negative bacteria. The present study describes the first outbreak of XDR colistin- and carbapenem-resistant OXA-23/NDM-1-producing Acinetobacter baumannii (CCRAB) in European overseas territory of Reunion Island (France, Indian Ocean). Between April 2019 and June 2020, 13 patients admitted to the University Hospital of Reunion Island were involved in the outbreak, of whom 8 were infected and 6 died. Read More

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Nonoperative and Operative Soft-Tissue, Cartilage, and Bony Regeneration and Orthopaedic Biologics of the Shoulder: An Orthoregeneration Network (ON) Foundation Review.

Arthroscopy 2021 Jul 19. Epub 2021 Jul 19.

Midwest Orthopaedics at Rush, Chicago, IL, USA. Electronic address:

Orthoregeneration is defined as a solution for orthopaedic conditions that harnesses the benefits of biology to improve healing, reduce pain, improve function, and optimally, provide an environment for tissue regeneration. Options include drugs, surgical intervention, scaffolds, biologics as a product of cells, and physical and electro-magnetic stimuli. The goal of regenerative medicine is to enhance the healing of tissue after musculoskeletal injuries as both isolated treatment and adjunct to surgical management, using novel therapies to improve recovery and outcomes. Read More

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Purification and characterization of an amyloidogenic repeat domain from the functional amyloid Pmel17.

Protein Expr Purif 2021 Jul 19:105944. Epub 2021 Jul 19.

Laboratory of Protein Conformation and Dynamics, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, United States. Electronic address:

The pre-melanosomal protein (Pmel17) is a human functional amyloid that supports melanin biosynthesis within melanocytes. This occurs in the melanosome, a membrane-bound organelle with an acidic intraluminal pH. The repeat region of Pmel17 (RPT, residues 315-444) has been previously shown to form amyloid aggregates under acidic melanosomal conditions, but not under neutral cytosolic conditions, when expressed and purified using a C-terminal hexa-histidine tag (RPT-His). Read More

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Formulation development, in vitro and in vivo evaluation of topical hydrogel formulation of econazole nitrate-loaded β-cyclodextrin nanosponges.

J Pharm Sci 2021 Jul 19. Epub 2021 Jul 19.

Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, 110062, India. Electronic address:

Econazole nitrate, an antifungal drug used in the handling of skin ailments, is commercially not efficient as these ailments typically require a more elevated concentration of the drug to offer an effective pharmacological retort. Like so, it is proposed to assess the effectiveness of the topical hydrogel of econazole-loaded nanosponge in the management of skin ailment(s). Econazole nitrate-laden β-cyclodextrin nanosponges were developed by employing the melt method using β-cyclodextrin as the organic polymer and N,N-carbonyldiimidazole as the crosslinker. Read More

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Design and evaluation of GLP-1 receptor G-protein biased agonist with prolonged efficacy on diabetes.

Life Sci 2021 Jul 19:119844. Epub 2021 Jul 19.

Peking University Third Hospital, Beijing 100191, PR China. Electronic address:

Aim: Glucagon-like peptide-1 receptor agonists (GLP-1RAs) have emerged as potent drug development target for treating diabetes and obesity. Here, we designed and evaluated novel long-acting GLP-1R G protein biased agonist with potent clinical application.

Main Methods: GLP-1R G-protein biased sequences were screened via a high-throughput autocrine-based strategy and then fused to Exendin (9-39). Read More

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Highly Branched Poly(β-amino ester)s for Gene Delivery in Hereditary Skin Diseases.

Adv Drug Deliv Rev 2021 Jul 19:113842. Epub 2021 Jul 19.

Charles Institute of Dermatology, University College Dublin, Dublin 4, Ireland. Electronic address:

Non-viral gene therapy for hereditary skin diseases is an attractive prospect. However, research efforts dedicated to this area are rare. Taking advantage of the branched structural possibilities of polymeric vectors, we have developed a gene delivery platform for the treatment of an incurable monogenic skin disease - recessive dystrophic epidermolysis bullosa (RDEB) - based on highly branched poly(β-amino ester)s (HPAEs). Read More

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Effect of Early Feeding on Intestinal Permeability and Inflammation Markers in Infants with Genetic Susceptibility to Type 1 Diabetes: A Randomized Clinical Trial.

J Pediatr 2021 Jul 19. Epub 2021 Jul 19.

Pediatric Research Center, Children's Hospital, University of Helsinki and Helsinki University Hospital, Helsinki, Finland; Research Program for Clinical and Molecular Metabolism, Faculty of Medicine, University of Helsinki, Helsinki, Finland; Center for Child Health Research, University of Tampere and Tampere University Hospital, Tampere, Finland; Department of Pediatrics, Tampere University Hospital, Tampere, Finland. Electronic address:

Objectives: To assess whether weaning to an extensively hydrolyzed formula (EHF) decreases gut permeability and/or markers of intestinal inflammation in infants with HLA-conferred diabetes susceptibility, when compared with conventional formula.

Study Design: By analyzing 1468 expecting biological parent pairs for HLA-conferred susceptibility for type 1 diabetes, 465 couples (32%) potentially eligible for the study were identified. After further parental consent, 332 babies to be born were randomized at 35 gestational week. Read More

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Subclinical Liver Disease is Associated with Subclinical Atherosclerosis in Psoriasis: Results from Two Observational Studies.

J Invest Dermatol 2021 Jul 19. Epub 2021 Jul 19.

National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA. Electronic address:

Psoriasis is associated with a higher risk of liver diseases. We investigated the impact of hepatic steatosis (European cohort) and hepatic inflammation (United States cohort) on subclinical atherosclerosis. In the European cohort (n=76 psoriasis participants and 76 controls), non-alcoholic fatty liver disease (NAFLD), assessed by the sonographic hepatorenal index (SHRI), was more prevalent in psoriasis than controls (61% vs 45%; p=. Read More

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PIP AQUAPORINS pH sensing is regulated by the length and charge of the C-terminal region.

FEBS J 2021 Jul 22. Epub 2021 Jul 22.

Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Fisicomatemática, Argentina.

Plant PIP aquaporins play a central role in controlling plant water status. The current structural model for PIP pH-gating states that the main pH sensor is located in loopD and that all the mobile cytosolic elements participate in a complex interactions network that ensures the closed structure. However, the precise participation of the last part of the C-terminal domain (CT) in PIP pH-gating remains unknown. Read More

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Transient hypertriglyceridaemia associated with propranolol for treatment of infantile hemangioma.

Australas J Dermatol 2021 Jul 22. Epub 2021 Jul 22.

Department of Dermatology, Wayne State University, Detroit, Michigan, USA.

We present a case of a one-month-old female patient with severe hypertriglyceridaemia as a side effect of treating an ulcerating infantile hemangioma with systemic propranolol. The remarkedly rapid increase in triglyceride returned to normal 96 hours after the discontinuation of the medication, and further follow-up revealed normalisation of the lipid profile. Further research is necessary to unveil the association of systemic propranolol with hypertriglyceridaemia. Read More

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Determination of β-Galactooligosaccharides (GOS) in Infant Formula and Adult Nutritionals: Single Laboratory Validation, First Action 2021.01.

J AOAC Int 2021 Jul 22. Epub 2021 Jul 22.

Société des Produits Nestlé S.A., Nestlé Research, Vers-Chez-Les-Blanc, 1000, Lausanne, Switzerland.

Background: β-Galactooligosaccharides (GOS) are typically used in infant formula and adult nutritionals as a source of non-digestible oligosaccharides, which may bring beneficial effects through modulation of the gut microbiota. However suitable methods for the determination of GOS in products with a high background of lactose do not exist.

Objective: The aim of this work was to develop a method suitable for the determination of GOS in infant formula and adult nutritionals and demonstrate suitability through single laboratory validation. Read More

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Male microchimerism in females: a quantitative study of twin pedigrees to investigate mechanisms.

Hum Reprod 2021 Jul 19. Epub 2021 Jul 19.

Amsterdam Reproduction and Development (AR&D) Research Institute, Amsterdam UMC, Vrije Universiteit, Amsterdam, The Netherlands.

Study Question: Does having a male co-twin, older brothers, or sons lead to an increased probability of persistent male microchimerism in female members of twin pedigrees?

Summary Answer: The presence of a male co-twin did not increase risk of male microchimerism and the prevalence of male microchimerism was not explained by having male offspring or by having an older brother.

What Is Known Already: Microchimerism describes the presence of cells within an organism that originate from another zygote and is commonly described as resulting from pregnancy in placental mammals. It is associated with diseases with a female predilection including autoimmune diseases and pregnancy-related complications. Read More

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Microtubules and Gαo-signaling modulate the preferential secretion of young insulin secretory granules in islet β cells via independent pathways.

PLoS One 2021 22;16(7):e0241939. Epub 2021 Jul 22.

Department of Cell and Developmental Biology, The Program of Developmental Biology and the Center for Stem Cell Biology, Vanderbilt University, Nashville, TN, United States of America.

For sustainable function, each pancreatic islet β cell maintains thousands of insulin secretory granules (SGs) at all times. Glucose stimulation induces the secretion of a small portion of these SGs and simultaneously boosts SG biosynthesis to sustain this stock. The failure of these processes, often induced by sustained high-insulin output, results in type 2 diabetes. Read More

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The effects of Cstb duplication on APP/amyloid-β pathology and cathepsin B activity in a mouse model.

PLoS One 2021 22;16(7):e0242236. Epub 2021 Jul 22.

UK Dementia Research Institute at UCL, London, United Kingdom.

People with Down syndrome (DS), caused by trisomy of chromosome 21 have a greatly increased risk of developing Alzheimer's disease (AD). This is in part because of triplication of a chromosome 21 gene, APP. This gene encodes amyloid precursor protein, which is cleaved to form amyloid-β that accumulates in the brains of people who have AD. Read More

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Self-Immolative System for Disclosure of Reactive Electrophilic Alkylating Agents: Understanding the Role of the Reporter Group.

J Org Chem 2021 Jul 22. Epub 2021 Jul 22.

Department of Chemistry, University of Reading, Whiteknights, Reading RG6 6AD, U.K.

The development of stable, efficient chemoselective self-immolative systems, for use in applications such as sensors, requires the optimization of the reactivity and degradation characteristics of the self-immolative unit. In this paper, we describe the effect that the structure of the reporter group has upon the self-immolative efficacy of a prototype system designed for the disclosure of electrophilic alkylating agents. The amine of the reporter group (a nitroaniline unit) was a constituent part of a carbamate that functioned as the self-immolative unit. Read More

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Cyclic, Hydrophobic Hexapeptide Fusahexin Is the Product of a Nonribosomal Peptide Synthetase in .

J Nat Prod 2021 Jul 22. Epub 2021 Jul 22.

Department of Chemistry and Bioscience, Aalborg University, Frederik Bajers Vej 7H, DK-9220 Aalborg, Denmark.

The plant pathogenic fungus is known to produce a wide array of secondary metabolites during plant infection. This includes several nonribosomal peptides. Recently, the fusaoctaxin (/9) and gramilin () gene clusters were shown to be induced by host interactions. Read More

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Solvent Relaxation NMR: A Tool for Real-Time Monitoring Water Dynamics in Protein Aggregation Landscape.

ACS Chem Neurosci 2021 Jul 22. Epub 2021 Jul 22.

Department of Biophysics, Bose Institute, Kolkata 700054, India.

Solvent dynamics strongly induce the fibrillation of an amyloidogenic system. Probing the solvation mechanism is crucial as it enables us to predict different proteins' functionalities, such as the aggregation propensity, structural flexibility, and toxicity. This work shows that a straightforward NMR method in conjunction with phenomenological models gives a global and qualitative picture of water dynamics at different concentrations and temperatures. Read More

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New Catalytic Radical Process Involving 1,4-Hydrogen Atom Abstraction: Asymmetric Construction of Cyclobutanones.

J Am Chem Soc 2021 Jul 22. Epub 2021 Jul 22.

Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467, United States.

While alkyl radicals have been well demonstrated to undergo both 1,5- and 1,6-hydrogen atom abstraction (HAA) reactions, 1,4-HAA is typically a challenging process both entropically and enthalpically. Consequently, chemical transformations based on 1,4-HAA have been scarcely developed. Guided by the general mechanistic principles of metalloradical catalysis (MRC), 1,4-HAA has been successfully incorporated as a key step, followed by 4-- radical substitution (RS), for the development of a new catalytic radical process that enables asymmetric 1,4-C-H alkylation of diazoketones for stereoselective construction of cyclobutanone structures. Read More

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Raman Imaging Reveals Accumulation of Hemoproteins in Plaques from Alzheimer's Diseased Tissues.

ACS Chem Neurosci 2021 Jul 22. Epub 2021 Jul 22.

Laboratoire de Bioélectrochimie et Spectroscopie, UMR 7140, Chimie De La Matière Complexe, Université de Strasbourg-CNRS, 4 Rue Blaise Pascal, 67081 Strasbourg, France.

Hemes have been suggested to play a central role in Alzheimer's disease since they show high peroxidase reactivity when bound to amyloid β peptides, leading to the production of reactive oxygen species. Here we used Fourier transform infrared and Raman imaging on Alzheimer's diseased mice and human brain tissue. Our finding suggests the accumulation of hemes in the senile plaques of both murine and human samples. Read More

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Role of antimicrobial peptides system in inflammatory periodontal diseases non-specific oral cavity protection.

Klin Lab Diagn 2021 Jul;66(7):422-427

FSBEI HE Northern State Medical University (Arkhangelsk) of the Ministry of Health of the Russian Federation.

The system of antimicrobial peptides (AMP) is one of the most ancient mechanisms of the macroorganism resistance to infectious pathogens invasion. The aim of the study was to determine the role of the antimicrobial peptides system and periodontal pathogenic markers in the development and progression of inflammatory periodontal diseases. Gingival pocket washes (91 samples in total) for the research were received from patients with inflammatory periodontal diseases (chronic periodontitis and gingivitis) and intact periodontium. Read More

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Plasma neurofilament light chain concentrations as a biomarker of clinical and radiologic outcomes in relapsing multiple sclerosis: Post hoc analysis of phase 3 ozanimod trials.

Eur J Neurol 2021 Jul 22. Epub 2021 Jul 22.

Mellen Center for MS Treatment and Research, Department of Neurology, Cleveland Clinic, Cleveland, OH, USA.

Background: We investigated plasma neurofilament light chain concentrations (pNfL) as a biomarker for neuroaxonal damage and disease activity using data from phase 3 trials of ozanimod in relapsing multiple sclerosis (RMS).

Methods: pNfL were measured before and after ozanimod 0.46 mg or 0. Read More

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