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The transcriptional characteristics of mast cells derived from skin tissue in type 2 diabetes patients at the single-cell level.

Acta Histochem 2021 Sep 21;123(7):151789. Epub 2021 Sep 21.

Wound Treatment Department, TCM-Integrated Hospital of Southern Medical University, Guangzhou, Guangdong Province, China. Electronic address:

Objectives: The mechanisms underlying the role of mast cells in wound healing have not been thoroughly studied, and even fewer data are available on studies related to mast cells in the skin of patients with type 2 diabetes mellitus (T2DM). Therefore, this study aims to explore the transcriptional characteristics of mast cells at the single-cell level in patients with T2DM and provide experimental data for studying mast cell behaviors under abnormal glucose metabolism.

Methods: Two patients with T2DM and one trauma patient without diabetes were enrolled. Read More

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September 2021

ATP-binding cassette transporter subfamily C members 2, 3 and cadherin protein are susceptibility-determining factors in Bombyx mori for multiple Bacillus thuringiensis Cry1 toxins.

Insect Biochem Mol Biol 2021 Sep 21:103649. Epub 2021 Sep 21.

Institute of Agrobiological Sciences, NARO, 1-2 Ohwashi, Tsukuba, Ibaraki 305-8634, Japan. Electronic address:

Field-evolved resistance of insect pests to Bacillus thuringiensis (Bt) toxins (Cry toxins) is a threat to the efficacy of Bt-based bio-insecticides and transgenic crops. Recent reports have suggested that ATP-binding cassette transporter subfamily C2 (ABCC2) and cadherin-like receptor play important roles in conferring susceptibility to Cry1 toxins. However, the receptors involved in Bt susceptibility in each insect remain unclear. Read More

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September 2021

Multivalent nanobody as capture antibody-based enzyme linked immunosorbent assay for detection of 3-phenoxybenzoic acid in urine.

Anal Biochem 2021 Sep 21:114390. Epub 2021 Sep 21.

College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030810, PR China. Electronic address:

Nanobodies (Nbs) as capture antibodies in enzyme-linked immunosorbent assays (ELISAs) is greatly hampered by their poor performance after attaching onto polystyrene microplates. Reasons behind those phenomena remain unknown. One of possible explanation is that Nbs with a single domain might lose their accessibility of paratope when adsorbed on the plates. Read More

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September 2021

Analysis of changes in the volume of edema around brain contusions and the influencing factors: A single-center, retrospective, observational study.

Medicine (Baltimore) 2021 Sep;100(38):e27246

Department of Neurosurgery of the 900th Hospital, Fuzong Clinical Medical School of Fujian Medical University, Fuzhou, Fujian Province, China.

Abstract: Traumatic brain injury (TBI), a common neurosurgical condition, has well-known treatment guidelines. However, the mechanisms underlying the varying severity of brain edema secondary to TBI are largely unknown, leading to controversial treatments.This study seeks to measure edema volumes around brain contusions in different regions, analyze factors related to differences in edema volume and provide a theoretical basis for brain edema treatment. Read More

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September 2021

The effectiveness and safety of Chuna manual therapy adjuvant to Western medicine in patients with chronic obstructive pulmonary disease: Study protocol for a randomized, single-blind, investigator-initiated, pilot trial.

Medicine (Baltimore) 2021 Sep;100(38):e27217

Department of Clinical Korean Medicine, Graduate School, Kyung Hee University, Dongdaemun-gu, Seoul, Republic of Korea.

Background: In chronic obstructive pulmonary disease (COPD) management, greater emphasis has been placed on symptomatic improvement and enhanced quality of life in patients. Manual therapy among respiratory rehabilitation programs has received much attention recently, with the publication of numerous studies. In South Korea, a method known as Chuna Manual Therapy (CMT) has been applied in the management of COPD patients and in clinical practice, but the clinical basis for safety and effectiveness is yet to be established. Read More

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September 2021

Integrative Analysis of mA Regulator-Mediated RNA Methylation Modification Patterns and Immune Characteristics in Lupus Nephritis.

Front Cell Dev Biol 2021 7;9:724837. Epub 2021 Sep 7.

Department of Nephrology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Background: There is growing evidence to demonstrate that the epigenetic regulation of immune characteristics, especially for N6-methyladenosine (mA) RNA methylation. However, how mA methylation is involved in lupus nephritis (LN) is still unclear. This study aimed to determine the role of mA RNA methylation and their association with the immune microenvironment in LN. Read More

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September 2021

Association of CNVs with methylation variation.

NPJ Genom Med 2020 Sep 24;5(1):41. Epub 2020 Sep 24.

Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, 02115, USA.

Germline copy number variants (CNVs) and single-nucleotide polymorphisms (SNPs) form the basis of inter-individual genetic variation. Although the phenotypic effects of SNPs have been extensively investigated, the effects of CNVs is relatively less understood. To better characterize mechanisms by which CNVs affect cellular phenotype, we tested their association with variable CpG methylation in a genome-wide manner. Read More

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September 2020

Self-catalytic growth of one-dimensional materials within dislocations in gold.

Proc Natl Acad Sci U S A 2021 Sep;118(39)

Department of Materials Science and Engineering, Technion-Israel Institute of Technology, 32000 Haifa, Israel;

Dislocations in metals affect their properties on the macro- and the microscales. For example, they increase a metal's hardness and strength. Dislocation outcrops exist on the surfaces of such metals, and atoms in the proximity of these outcrops are more loosely bonded, facilitating local chemical corrosion and reactivity. Read More

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September 2021

Understanding Symmetry Breaking at the Single-Particle Level the Growth of Tetrahedron-Shaped Nanocrystals from Higher-Symmetry Precursors.

ACS Nano 2021 Sep 23. Epub 2021 Sep 23.

The vast majority of single crystalline metal nanoparticles adopt shapes in the point group as a consequence of the symmetry of the underlying face-centered cubic (FCC) crystal lattice. Tetrahedra are a notable exception to this rule, and although they have been observed in several syntheses, their growth mechanism, and the symmetry-reduction process that necessarily characterizes it, is poorly understood. Here, a symmetry breaking mechanism is revealed by liquid flow cell transmission electron microscopy (TEM) observation of seeded growth in which tetrahedra nanoparticles are formed from higher symmetry seeds. Read More

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September 2021

Treatment Impact on Patient-Reported Outcomes in Peripheral Neuropathic Pain: Comparing Single Intervention With Topical High-Concentration Capsaicin to Daily Oral Pregabalin.

Pain Physician 2021 Sep;24(6):453-463

Grünenthal GmbH, Aachen, Germany.

Background: Peripheral neuropathic pain (PNP) is a complex, subjective experience affecting both physical and psychological aspects of functioning. Assessing patient-reported outcomes (PROs) beyond pain relief is important and aligns with the recommendations of IMMPACT (Initiative on Methods, Measurement, and Pain Assessment in Clinical Trials). Moreover, PRO data are key to clinical decision-making when evaluating treatment options. Read More

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September 2021

Allelic variation in candidate genes associated with wood properties of cultivated poplars (Populus).

Biol Futur 2019 Dec 1;70(4):286-294. Epub 2019 Dec 1.

Simonyi Karoly Faculty of Engineering, Wood Sciences and Applied Arts, University of Sopron, Bajcsy Zs. u. 4, 9400, Sopron, Hungary.

Introduction: Since Populus has veritable value as timber, plywood, pulp, and paper, genomic research should create the sound basis for further breeding toward desirable wood quality attributes.

Materials And Methods: In this study, we addressed the need for a research methodology that initially identifies and then characterize candidate genes encoding enzymes with wood property phenotypic traits, toward the aim of developing a genomics-based breeding technology.

Results: On 23 different poplar species/hybrid samples, we successfully amplified 55 primers designed on Populus trichocarpa L. Read More

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December 2019

HTL-Free Sb(S, Se) Solar Cells with an Optimal Detailed Balance Band Gap.

ACS Appl Mater Interfaces 2021 Sep 23. Epub 2021 Sep 23.

Wuhan National Laboratory for Optoelectronics and School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China.

Antimony chalcogenides are widely studied as a light-absorbing material due to their merits of low toxicity, efficient cost, and excellent photovoltaic properties. However, the band gaps of antimony selenide (approximately 1.1 eV) and antimony sulfide (approximately 1. Read More

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September 2021

EZH2 identifies the precursors of human natural killer cells with trained immunity.

Cancer Biol Med 2021 Sep 24. Epub 2021 Sep 24.

Department of Immunology and Biotherapy, Key Laboratory of Cancer Immunology and Biotherapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin's Clinical Research Center for Cancer, Tianjin 300060, China.

Objective: Trained immunity of natural killer (NK) cells has shown great potential in the treatment of cancers by eliciting enhanced effector responses to restimulation by cytokines or cancer cells for long time periods after preactivation. However, the human NK cells responsible for the generation and maintenance of trained immunity are largely unknown. We hypothesized that heterogeneous human NK cells would respond differentially to stimulation with a combination of IL-12, IL-15, and IL-18, and that an NK cell subset might exist that is mainly responsible for the induction of trained immunity. Read More

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September 2021

Evaluation of patient compliance with the use of scrotal cooling devices.

F S Rep 2021 Sep 30;2(3):289-295. Epub 2021 Jun 30.

Division of Urology, Department of Surgery, University of Toronto, Toronto, Ontario, Canada.

Objective: To evaluate the compliance of infertile men with the use of scrotal cooling devices. As a secondary objective, sperm parameters, deoxyribonucleic acid fragmentation, and hormone profiles were examined.

Design: This exploratory study on scrotal cooling provided scrotal cooling devices to men with primary infertility and abnormal semen parameters. Read More

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September 2021

Tunable Wide-Field Illumination and Single-Molecule Photoswitching with a Single MEMS Mirror.

ACS Photonics 2021 Sep 25;8(9):2728-2736. Epub 2021 Aug 25.

Department of Physics, SUPA, University of Strathclyde, Glasgow, Scotland, United Kingdom.

Homogeneous illumination in single-molecule localization microscopy (SMLM) is key for the quantitative analysis of super-resolution images. Therefore, different approaches for flat-field illumination have been introduced as alternative to the conventional Gaussian illumination. Here, we introduce a single microelectromechanical systems (MEMS) mirror as a tunable and cost-effective device for adapting wide-field illumination in SMLM. Read More

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September 2021

Changes in barriers to implementing early mobilization in the intensive care unit: a single center retrospective cohort study.

Nagoya J Med Sci 2021 Aug;83(3):443-464

Department of Intensive Care Medicine, Showa University School of Medicine, Tokyo, Japan.

This study was undertaken to investigate the rate of mobilization, defined as a rehabilitation level of sitting on the edge of a bed or higher, and its association with changes in barriers in the intensive care unit (ICU). Consecutive patients from January 2016 to March 2019 admitted to the ICU, 18 years old or older, who did not meet exclusion criteria, were eligible. The primary outcome was the rate of mobilization. Read More

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Efficient implementations of the symmetric and asymmetric triple excitation corrections for the orbital-optimized coupled-cluster doubles method with the density-fitting approximation.

J Chem Phys 2021 Sep;155(11):114104

Department of Chemistry, Hacettepe University, Ankara 06800, Turkey.

Efficient implementations of the symmetric and asymmetric triple excitation corrections for the orbital-optimized coupled-cluster doubles (OCCD) method with the density-fitting approach, denoted by DF-OCCD(T) and DF-OCCD(T), are presented. The computational cost of the DF-OCCD(T) method is compared with that of the conventional OCCD(T). In the conventional OCCD(T) and OCCD(T) methods, one needs to perform four-index integral transformations at each coupled-cluster doubles iterations, which limits its applications to large chemical systems. Read More

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September 2021

The curious case of DMSO: A CCSD(T)/CBS(aQ56+d) benchmark and DFT study.

J Chem Phys 2021 Sep;155(11):114304

Department of Chemistry, Mississippi State University, Mississippi State, Mississippi 39762-9573, USA.

This work addresses the pathological behavior of the energetics of dimethyl sulfoxide and related sulfur-containing compounds by providing the computational benchmark energetics of RE species, where R = H/CH and E = O/S, with bent and pyramidal geometries using state-of-the-art methodologies. These 22 geometries were fully characterized with coupled-cluster with single, double, and perturbative triple excitations [CCSD(T)], second-order Møller-Plesset perturbation theory (MP2), and 22 density functional theory (DFT) methods with 8, 12, and 12, respectively, correlation consistent basis sets of double-, triple-, or quadruple-ζ quality. The relative energetics were determined at the MP2 and CCSD(T) complete basis set (CBS) limits using 17 basis sets up to sextuple-ζ and include augmented, tight-d, and core-valence correlation consistent basis sets. Read More

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September 2021

Spin-free formulation of the multireference driven similarity renormalization group: A benchmark study of first-row diatomic molecules and spin-crossover energetics.

J Chem Phys 2021 Sep;155(11):114111

Department of Chemistry and Cherry Emerson Center for Scientific Computation, Emory University, Atlanta, Georgia 30322, USA.

We report a spin-free formulation of the multireference (MR) driven similarity renormalization group (DSRG) based on the ensemble normal ordering of Mukherjee and Kutzelnigg [J. Chem. Phys. Read More

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September 2021

High accuracy ab initio potential energy surface for the HO-H van der Waals dimer.

J Chem Phys 2021 Sep;155(11):114302

Department of Chemistry, University of Tennessee, Knoxville, Tennessee 37996-1600, USA.

A representation of the three-dimensional potential energy surface (PES) of the HO-H van der Waals dimer is presented. The HO molecule is treated as a rigid body held at its experimentally determined equilibrium geometry, with the OH bond length set to 1.809 650 34 a and the HOH bond angle set to 1. Read More

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September 2021

Effects of increasing dietary inclusion of camelina cake on growth performance of growing-finishing pigs.

Transl Anim Sci 2021 Jul 26;5(3):txab140. Epub 2021 Aug 26.

West Central Research and Outreach Center, University of Minnesota, Morris, MN, 56267, USA.

The objective of this experiment was to determine the dietary inclusion rate of camelina cake (CC) that would support the growth performance of growing-finishing pigs similar to that of a corn-soybean meal-based diet. Pigs ( = 192; BW = 35.2 kg; Duroc x (Yorkshire x Landrace)), balanced for sex and initial weight, were assigned to pens (8 pigs/pen) and pens were assigned randomly to one of four dietary treatments (6 pens/treatment). Read More

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A single mutation weakens symbiont-induced reproductive manipulation through reductions in deubiquitylation efficiency.

Proc Natl Acad Sci U S A 2021 09;118(39)

Division of Biological Sciences, University of Montana, Missoula, MT 59801

Animals interact with microbes that affect their performance and fitness, including endosymbionts that reside inside their cells. Maternally transmitted bacteria are the most common known endosymbionts, in large part because of their manipulation of host reproduction. For example, many cause cytoplasmic incompatibility (CI) that reduces host embryonic viability when -modified sperm fertilize uninfected eggs. Read More

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September 2021

A single-cell tumor immune atlas for precision oncology.

Genome Res 2021 Sep 21. Epub 2021 Sep 21.

CNAG-CRG, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain.

The tumor immune microenvironment is a main contributor to cancer progression and a promising therapeutic target for oncology. However, immune microenvironments vary profoundly between patients, and biomarkers for prognosis and treatment response lack precision. A comprehensive compendium of tumor immune cells is required to pinpoint predictive cellular states and their spatial localization. Read More

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September 2021

Treatment of acute aneurysmal subarachnoid haemorrhage with primary flow diversion: 5-year single-centre experience.

Clin Radiol 2021 Sep 18. Epub 2021 Sep 18.

Neuroradiology Department, Royal Victoria Hospital, Belfast, UK.

Aim: To evaluate the safety and efficacy of treatment of patients presenting with acute aneurysmal subarachnoid haemorrhage (SAH) with primary flow-diverting stents (FDS; with or without adjuncts), with comparison to the published literature.

Materials And Methods: A retrospective single-centre review was undertaken of prospectively obtained data on patients treated for SAH over a 60-month period. Of 354 patients treated for SAH during that time period, 24 patients with a total of 25 aneurysms were identified. Read More

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September 2021

Clinico-etiological Profile of Melasma among Female Health Workers in a Tertiary Care Center of Central Nepal - A Cross Sectional Study.

Authors:
D Karn A Subedi S Kc

Kathmandu Univ Med J (KUMJ) 2017 Apr-Jun;17(58):160-163

Department of Dermatology, Dhulikhel Hospital, Kathmandu University Hospital, Dhulikhel, Kavre, Nepal.

Background Melasma is an acquired symmetrical dyschromia with profound psychosocial impacts. It is a common pigmentary disorder with less clear etiology and limited management options. There are limited data regarding melasma in our scenario. Read More

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September 2021

Single and simultaneous effects of naphthalene and salinity on anaerobic digestion: Response surface methodology, microbial community analysis and potential functions prediction.

Environ Pollut 2021 Sep 16;291:118188. Epub 2021 Sep 16.

School of Environmental Science and Engineering, China-America CRC for Environment & Health, Shandong University, 72#Jimo Binhai Road, Qingdao, Shandong Province, 266237, China. Electronic address:

Polycyclic aromatic hydrocarbons (PAHs) are a persistent and prevalent class of pollutants in petroleum-contaminated saline environment, which pose potential harm to organisms. Researches on anaerobic biodegradation of PAHs are gradually emerging, but the response of anaerobic microorganisms to salinity changes and the co-effects of salinity and PAHs in anaerobic digestion (AD) system have seldom been reported. Thus, we investigated the variations of AD system performance and anaerobic microbial community caused by different concentrations of naphthalene (Nap) or/and NaCl based on Box-Behnken Design (0 mg/L ≤ Nap ≤150 mg/L, 0 g/L ≤ NaCl ≤25 g/L). Read More

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September 2021

The nedd-8 activating enzyme gene underlies genetic resistance to infectious pancreatic necrosis virus in Atlantic salmon.

Genomics 2021 Sep 18. Epub 2021 Sep 18.

The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian EH25 9RG, UK. Electronic address:

Genetic resistance to infectious pancreatic necrosis virus (IPNV) in Atlantic salmon is a rare example of a trait where a single locus (QTL) explains almost all of the genetic variation. Genetic marker tests based on this QTL on salmon chromosome 26 have been widely applied in selective breeding to markedly reduce the incidence of the disease. In the current study, whole genome sequencing and functional annotation approaches were applied to characterise genes and variants in the QTL region. Read More

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September 2021

Assessing Density Functional Theory for Chemically Relevant Open-Shell Transition Metal Reactions.

J Chem Theory Comput 2021 Sep 21. Epub 2021 Sep 21.

Mulliken Center for Theoretical Chemistry, Institute for Physical and Theoretical Chemistry, University of Bonn, Beringstr. 4, 53115 Bonn, Germany.

Due to the principle lack of systematic improvement possibilities of density functional theory, careful assessment of the performance of density functional approximations (DFAs) on well-designed benchmark sets, for example, for reaction energies and barrier heights, is crucial. While main-group chemistry is well covered by several available sets, benchmark data for transition metal chemistry is sparse. This is especially the case for larger, chemically relevant molecules. Read More

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September 2021

The Acute Effect of Packed Red Blood Cell Transfusion in Mechanically Ventilated Children after the Norwood Operation.

Pediatr Cardiol 2021 Sep 21. Epub 2021 Sep 21.

Division of Cardiology, Advocate Children's Hospital, Chicago, IL, USA.

Packed red blood cell (PRBC) transfusions are commonly administered in pediatric patients following the Norwood operation. This study was conducted to determine the effect of PRBC transfusions on hemodynamic parameters in pediatric patients with single-ventricle physiology and parallel circulation. A single-center, retrospective chart review was conducted. Read More

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September 2021