965 results match your criteria bacteria subcultured

Helicobacter canis bacteraemia in a rheumatoid arthritis patient treated with tofacitinib: case report and literature review.

Ann Clin Microbiol Antimicrob 2021 Apr 8;20(1):22. Epub 2021 Apr 8.

Division of Internal Medicine, Department of Rheumatology, University Medical Centre Maribor, Ljubljanska ulica 5, 2000, Maribor, Slovenia.

Background: Non-Helicobacter pylori species (NHPS) are newly emerging bacteria that naturally inhabit birds and mammals apart from humans and rarely cause diseases in humans. In recent years, a rise in the number of cases associated with NHPS infections in humans has been observed. Among them, infections with Helicobacter (H. Read More

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Heterogeneity of Strains Isolated from Patients with Gastric Disorders in Guiyang, China.

Infect Drug Resist 2021 11;14:535-545. Epub 2021 Feb 11.

Key Laboratory of Microbiology and Parasitology of Education Department of Guizhou, School of Basic Medical Science, Guizhou Medical University/Joint Laboratory of Helicobacter Pylori and Intestinal Microecology of Affiliated Hospital of Guizhou Medical University, Guiyang, People's Republic of China.

Purpose: Chronic infection causes peptic ulcers in a subpopulation of individuals and is a risk factor for the development of gastric cancer. Multiple infections and heteroresistant contribute to poor treatment efficacy. Here, we investigated the extent of genetic diversity among strains within a given host and its influence on the results of antibiotic (metronidazole, levofloxacin, clarithromycin, amoxicillin, and tetracycline) susceptibility testing. Read More

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February 2021

A stress-induced block in dicarboxylate uptake and utilization in .

J Bacteriol 2021 Feb 16. Epub 2021 Feb 16.

Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada

Bacteria have evolved to sense and respond to their environment by altering gene expression and metabolism to promote growth and survival. In this work we demonstrate that displays an extensive (>30 hour) lag in growth when subcultured into media where dicarboxylates such as succinate are the sole carbon source. This growth lag is regulated in part by RpoS, the RssB anti-adaptor IraP, translation elongation factor P, and to a lesser degree the stringent response. Read More

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February 2021

Soybean-associated endophytic fungi as potential source for anti-COVID-19 metabolites supported by docking analysis.

J Appl Microbiol 2021 Feb 8. Epub 2021 Feb 8.

Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt.

Aims: To identify the metabolites produced by the endophytic fungus, Aspergillus terreus and to explore the anti-viral activity of the identified metabolites against the pandemic disease COVID-19 in-silico.

Methods And Results: Herein, we reported the isolation of A. terreus, the endophytic fungus associated with soybean roots, which is then subcultured using OSMAC approach in five different culture media. Read More

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February 2021

Unique method for human villous trophoblasts isolation from placental tissue explants.

Clin Obstet Gynecol Reprod Med 2020 Dec 10;6(6). Epub 2020 Nov 10.

Center for AIDS Health Disparities Research, Department of Microbiology, Immunology, and Physiology, Meharry Medical College, School of Medicine, Nashville, TN, USA.

Isolation of cytotrophoblasts from primary placental tissue may be costly and time consuming with variable results. In this paper, we provide a simple, affordable, and efficient method that may performed using common laboratory supplies to achieve consistent isolation of cytotrophoblasts from villous tissue. Trophoblast populations are identified based on morphology and phenotyping, which employs the timely extraction of villous nodes from the placenta prior to cultivation and isolation of nodal outgrowth by visual guidance for selective capture of cytotrophoblast populations and subculture. Read More

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December 2020

Thousand cankers disease in : Optimizing sampling and identification procedures for the vector , and the causal agent .

MethodsX 2020 3;7:101174. Epub 2020 Dec 3.

Department of Agricultural, Food, Environmental and Forestry Science and Technology (DAGRI), Plant Pathology and Entomology section, University of Florence, Italy.

Lindgren funnel traps were used to monitor occurrence Traps were placed directly on walnut trees, with the top tied to one of the lower branches (about 2m high). An 8-funnel model was used instead of a 4-funnel trap, with the specific pheromone bait positioned between the fourth and the fifth funnel. Traps were customized with a 5mm metal mesh which was placed inside the bottom funnel so that debris (mainly foliage) and larger non-target insects would not end up inside the collecting jar. Read More

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December 2020

Isolation of human coronaviruses OC43, HKU1, NL63, and 229E in Yamagata, Japan, using primary human airway epithelium cells cultured by employing an air-liquid interface culture.

Jpn J Infect Dis 2020 Nov 30. Epub 2020 Nov 30.

Department of Microbiology, Yamagata Prefectural Institute of Public Health, Japan.

Isolation of seasonal coronaviruses, which include human coronavirus (HCoV) OC43, HCoV-HKU1, and HCoV-NL63, from primary cultures is difficult because it requires experienced handling, an exception being HCoV-229E, which can be isolated using cell lines such as RD-18S and HeLa-ACE2-TMPRSS2. We aimed to isolate seasonal CoVs in Yamagata, Japan to obtain infective virions useful for further research and to accelerate fundamental studies on HCoVs and SARS-CoV-2. Using modified air-liquid interface (ALI) culture of the normal human airway epithelium from earlier studies, we isolated 29 HCoVs (80. Read More

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November 2020

Characterization of a new cell line from ornamental fish Amphiprion ocellaris (Cuvier, 1830) and its susceptibility to nervous necrosis virus.

Sci Rep 2020 11 18;10(1):20051. Epub 2020 Nov 18.

Fish Genetics and Biotechnology Division, ICAR-Central Institute of Fisheries Education, Mumbai, 400061, India.

Amphiprion ocellaris (ocellaris clownfish) is one of the most commercially important marine ornamental fish. A cell line designated as OCF was developed for the first time from the caudal fin of this fish species. The cell line was maintained in Leibovitz's-15 medium supplemented with 15% FBS (Fetal Bovine Serum) and was successfully subcultured up to 34 passages. Read More

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November 2020

Rapid method for direct identification of positive blood cultures by MALDI-TOF MS.

Exp Ther Med 2020 Dec 16;20(6):235. Epub 2020 Oct 16.

Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, P.R. China.

Application of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) using positive blood cultures (BCs) is a revolution in identification of microorganisms in clinical microbiology laboratories. Although there are several commercial pretreatment protocols they are expensive. Here, we evaluated the performance of a locally produced Bioyong pre-treatment kit for the direct identification of microorganisms in positive BCs by MALDI-TOF MS method. Read More

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December 2020

Antibiotics Profile, Prevalence of Extended-Spectrum Beta-Lactamase (ESBL), and Multidrug-Resistant from Different Clinical Samples in Khartoum State, Sudan.

Ehssan H Moglad

Int J Microbiol 2020 24;2020:8898430. Epub 2020 Oct 24.

Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O.Box 173, Alkharj 11942, Saudi Arabia.

One of the global requirements for controlling the occurrence of resistance to antimicrobial drugs is to understanding the resistivity profile of various clinical isolates. Therefore, this study aimed to deliver the indication of different resistant profiles of clinically isolated from different sources of samples from Khartoum state, Sudan, and to determine the prevalence rate of extended-spectrum beta-lactamase (ESBL), multidrug-resistant (MDR), extensively drug-resistant (XDR), and pandrug-resistant (PDR) bacteria. A total of 144 Gram-negative bacteria were collected from different sources (vaginal swab, urine, catheter tip, sputum, blood, tracheal aspirate, pus, stool, pleural fluid, and throat swab). Read More

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October 2020

Antimicrobial Resistance Patterns of Bacterial Isolates from Blood Culture among HIV/AIDS Patients at Felege Hiwot Referral Hospital, Northwest Ethiopia.

Int J Microbiol 2020 19;2020:8893266. Epub 2020 Oct 19.

Bahir Dar University, College of Medicine and Health Sciences, School of Health Sciences, Department of Medical Laboratory Sciences, Unit of Medical Microbiology, Bahir Dar, Ethiopia.

Background: The emergence and spread of antimicrobial resistance in bacteria is recognized as a global public health problem. Bloodstream infection with antimicrobial-resistant bacteria in HIV/AIDS patients makes the problem more challenging. So, regular and periodic diagnosis and use of the appropriate antimicrobial susceptibility pattern determination is the only option for decreasing the prevalence and development of drug-resistant bacteria. Read More

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October 2020

A pioneer calf foetus microbiome.

Sci Rep 2020 10 19;10(1):17712. Epub 2020 Oct 19.

Department of Physiology, Anatomy and Microbiology, La Trobe University, Melbourne, VIC, 3086, Australia.

Foetus sterility until parturition is under debate due to reports of microorganisms in the foetal environment and meconium. Sufficient controls to overcome sample contamination and provide direct evidence of microorganism viability in the pre-rectal gastrointestinal tract (GIT) have been lacking. We conducted molecular and culture-based analyses to investigate the presence of a microbiome in the foetal GIT of calves at 5, 6 and 7 months gestation, while controlling for contamination. Read More

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October 2020

In vitro plant regeneration and Agrobacterium-mediated genetic transformation of a carnivorous plant, Nepenthes mirabilis.

Sci Rep 2020 10 15;10(1):17482. Epub 2020 Oct 15.

Plant Advanced Technologies SA, 54500, Vandœuvre-lès-Nancy, France.

In nutrient-poor habitats, carnivorous plants have developed novel feeding strategies based on the capture and digestion of prey and the assimilation of prey-derived nutrients by specialized traps. The Nepenthes genus, comprising nearly 160 species, presents a remarkable pitcher-shaped trap, leading to great interest among biologists, but the species of this genus are listed as threatened. In this work, we developed a protocol for reproducing Nepenthes mirabilis through shoot regeneration from calli. Read More

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October 2020

The effect of anti-tuberculosis drugs therapy on mRNA efflux pump gene expression of in collected from tuberculosis patients.

New Microbes New Infect 2019 Nov 8;32:100609. Epub 2019 Oct 8.

Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.

Efflux pumps are transmembrane proteins that vigorously participate in extruding a wide range of substrates, including drugs, outside the bacterial cell. We aimed to investigate the mRNA expression level of the efflux pump gene in isolated from individuals with tuberculosis who received drug therapy, at the 1st, 3rd and 5th months, and newly diagnosed patients with tuberculosis who will receive drug therapy (0 month). The study was a multiple cross-sectional longitudinal design-50 different isolates and a reference strain H37Rv were subcultured in LJ medium and confirmed by multiplex PCR for identification of and collected for RNA extraction. Read More

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November 2019

Performance of lipid fingerprint-based MALDI-ToF for the diagnosis of mycobacterial infections.

Clin Microbiol Infect 2020 Aug 27. Epub 2020 Aug 27.

MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, SW7 2AZ, UK. Electronic address:

Objectives: Bacterial diagnosis of mycobacteria is often challenging because of the variability of the sensitivity and specificity of the assay used, and it can be expensive to perform accurately. Although matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) has become the workhorse of clinical laboratories, the current MALDI methodology (which is based on cytosolic protein profiling) for mycobacteria is still challenging due to the number of steps involved (up to seven) and potential biosafety concerns. Knowing that mycobacteria produce surface-exposed species-specific lipids, we here hypothesized that the detection of those molecules could offer a rapid, reproducible and robust method for mycobacterial identification. Read More

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Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies.

Viruses 2020 08 18;12(8). Epub 2020 Aug 18.

Faculty of Science, University of South Bohemia in České Budějovice, 37005 České Budějovice, Czech Republic.

A highly virulent strain (Hypr) of tick-borne encephalitis virus (TBEV) was serially subcultured in the mammalian porcine kidney stable (PS) and tick (IRE/CTVM19) cell lines, producing three viral variants. These variants exhibited distinct plaque sizes and virulence in a mouse model. Comparing the full-genome sequences of all variants, several nucleotide changes were identified in different genomic regions. Read More

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Impact of Sequential Passaging on Protein Expression of Using Proteomics Analysis.

Int J Microbiol 2020 30;2020:2716202. Epub 2020 Jul 30.

Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80324, Jeddah 21859, Saudi Arabia.

Urinary tract infection (UTI) is one of the most prevalent bacterial infections in the world affecting the bladder and the kidney. () is the main causative agent of 80-90% of community-acquired UTIs, about 40% of nosocomial UTIs, and 25% of recurrent UTIs. The field of proteomics has emerged as a great tool to analyze expressed proteins to identify possible biomarkers associated with many pathological states and, to the same extent, those associated with bacterial pathogenesis and their ability to cause recurrent infections. Read More

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Impact of pH on succession of sourdough lactic acid bacteria communities and their fermentation properties.

Biosci Microbiota Food Health 2020 6;39(3):152-159. Epub 2020 Mar 6.

Laboratory of Microbial Technology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.

Sourdough, a traditional fermented dough, is made via natural fermentation by lactic acid bacteria (LAB). Its pH changes from near neutral to acid during the subculture process. However, the product quality of subcultured sourdough depends on the unpredictable succession of LAB communities, the influential factors of which are still unclear. Read More

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Comparative evaluation of various herbal and synthetic solutions on disinfection of guttapercha: An study.

Indian J Dent Res 2020 May-Jun;31(3):376-381

Department of Conservative Dentistry and Endodontics, Meghna Institute of Dental Sciences, Nizamabad, Telangana, India.

Background: Although gutta-percha cones are usually supplied in aseptic packages, once opened and used, they may be contaminated. Chair side disinfection of these cones is important and critical for success of endodontic therapy. Gutta-percha being heat labile, chemical disinfection is the only viable alternative for the sterilization of these cones. Read More

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Search for spp. Reveals High Prevalence and Pronounced Genetic Diversity of Arcobacter butzleri in Floodwater Samples Associated with Hurricane Florence in North Carolina, USA.

Appl Environ Microbiol 2020 10 1;86(20). Epub 2020 Oct 1.

North Carolina State University, Department of Food, Nutrition and Bioprocessing Sciences, Raleigh, North Carolina, USA

In September 2018, Hurricane Florence caused extreme flooding in eastern North Carolina, USA, a region highly dense in concentrated animal production, especially swine and poultry. In this study, floodwater samples ( = 96) were collected as promptly post-hurricane as possible and for up to approximately 30 days and selectively enriched for using Bolton broth enrichment and isolation on modified charcoal cefoperazone deoxycholate agar (mCCDA) microaerobically at 42°C. Only one sample yielded , which was found to be with the novel sequence type 2866 (ST-2866). Read More

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October 2020

and Comparison of Changes in Antibiotics Susceptibility of and Chicken's Intestinal Flora after Exposure to Amoxicillin or Thymol.

Vet Med Int 2020 7;2020:8824008. Epub 2020 Jul 7.

Department of Biology, Faculty of Science Dhar El-Mahraz, University Sidi Mohammed Ben Abdellah, P.O. Box 1796, Atlas, Fez, Morocco.

This study aims at verifying, , the extent to which the use of amoxicillin or thymol induces the selection of resistant bacteria and at evaluating their effects on the development of antimicrobial resistance in the intestinal flora of poultry. strain was subcultured on agar plates containing increasing concentrations of either amoxicillin or thymol. Thereafter, minimal inhibitory concentrations (MICs) of thymol, amoxicillin, and two other antibiotics, tylosin and colistin, were determined using the microdilution method. Read More

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Seasonal dynamics of the bacterial communities associated with cyanobacterial blooms in the Han River.

Environ Pollut 2020 Nov 9;266(Pt 2):115198. Epub 2020 Jul 9.

Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea University, Seoul, 02841, Republic of Korea. Electronic address:

DNA-based analyses of bacterial communities were performed to identify the bacteria co-occurring with cyanobacterial blooms in samples collected at a single site over 2 years. Microcystis aeruginosa was the most predominant species (81% in 2018, and 94% in 2019) within the phylum Cyanobacteria, and microcystins were detected during all cyanobacterial blooms. The stereo microscope and scanning electron microscope observations showed bacterial associations on and around the aggregated M. Read More

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November 2020

Candida albicans culture from a rectal swab can be associated with newly diagnosed colorectal cancer.

Folia Microbiol (Praha) 2020 Dec 29;65(6):989-994. Epub 2020 Jun 29.

Department of Microbiology, Faculty of Medicine & Dentistry, Palacký University Olomouc, Hněvotínská 3, Olomouc, Czech Republic.

Plenty of metagenomic studies have suggested possible associations between microbiome composition and colorectal cancer (CRC). However, these techniques are not economic enough for routine use so far. Therefore, we explored the possibility to detect species associated with colorectal cancer by conventional culture from rectal swab. Read More

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December 2020

Application of Phenotype Microarray for Profiling Carbon Sources Utilization between Biofilm and Non-Biofilm of Pseudomonas aeruginosa from Clinical Isolates.

Curr Pharm Biotechnol 2020 ;21(14):1539-1550

Department of Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

Background: This is the fastest work in obtaining the metabolic profiles of Pseudomonas aeruginosa in order to combat the infection diseases which leads to high morbidity and mortality rates. Pseudomonas aeruginosa is a high versatility of gram-negative bacteria that can undergo aerobic and anaerobic respiration. Capabilities in deploying different carbon sources, energy metabolism and regulatory system, ensure the survival of this microorganism in the diverse environment condition. Read More

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January 2021

Development of primary cell culture from spleen of giant gourami Osphronemus goramy for propagation of giant gourami iridovirus (GGIV).

J Fish Dis 2020 Aug 8;43(8):829-838. Epub 2020 Jun 8.

Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University (Bogor Agricultural University), Bogor, Indonesia.

The severe mortality of fish due to the infection of megalocytivirus caused significant economic losses. Since 2011, megalocytivirus (giant gourami iridovirus (GGIV)) has become the main pathogen in giant gourami (Osphronemus goramy), particularly in West Java, Central Java and Bali. This study aimed to develop primary cell culture from spleen as the target organ for propagating megalocytivirus in vitro, which was developed by explant method with enzymatic dissociation. Read More

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Bacteriological and Phytochemical Assessment of Linn. Infusion.

Biomed Res Int 2020 13;2020:9762639. Epub 2020 May 13.

Microbiology Department, Faculty of Science, Olabisi Onabanjo University, Ago Iwoye, Ogun State, Nigeria.

Linn. known as "Eepin" in Yoruba language, or sand paper tree, is a monoecious fig tree whose leaves, bark, seeds, and roots have been used locally in treating many infectious and noninfectious diseases. The study is aimed at investigating the bacteriological and phytochemical potential of Linn. Read More

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Isolation, culture and characterization of primary cell lines of human buccal mucosal fibroblasts: A combination of explant enzamytic technique.

J Oral Maxillofac Pathol 2020 Jan-Apr;24(1):68-75. Epub 2020 May 8.

Dr Prabhakar Kore Basic Science Research Center, KLE Academy of Higher Education and Research, Belgaum, Karnataka, India.

Background: The cell culture technique has become a routine and a popular method for its wide applications in the field of cell biology and biotechnology and in medical research. Isolation of primary cells over the cancer cells is an essential component of cell culture technology as they are the reliable source to understand normal physiological, morphological and molecular process of human cells. As fibroblasts are the prominent cells of the connective tissue of oral mucosa, many disease entities and histogenesis are linked to fibroblasts. Read More

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Prevalence of Asymptomatic Bacteriuria and Antibiotic Susceptibility Patterns of Bacterial Isolates among Cancer Patients and Healthy Blood Donors at the University of Gondar Specialized Hospital.

Int J Microbiol 2020 16;2020:3091564. Epub 2020 Apr 16.

Department of Medical Microbiology, School of Biomedical and Laboratory Sciences, University of Gondar, Gondar, Ethiopia.

Background: Urinary tract infections are the common types of infections in the community and health care settings. Despite the widespread availability of antibiotics, urinary tract infection remains a worldwide therapeutic problem. It is a continuous and significant problem in cancer patients. Read More

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High carriage rates of extended-spectrum beta-lactamase-producing in children at admission into paediatric wards of a university teaching hospital in Lagos, Nigeria.

Niger Postgrad Med J 2020 Apr-Jun;27(2):136-142

Research and Innovation Unit, University of Lagos, Akoka, Lagos, Nigeria.

Context: Bowel carriage has been identified as the main reservoir of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) and hospital-acquired infections. There are gaps in the knowledge of trends of these rates, which need to be filled for the development and implementation of hospital surveillance systems and antibiotic stewardship programmes in Nigeria.

Aim: This study investigated the carriage rates of ESBL-PE among 273 children admitted to the paediatric wards of a university teaching hospital, Nigeria, using a prospective cohort study design over a 6-month period. Read More

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Whole Genome Sequencing Differentiates Presumptive Extended Spectrum Beta-Lactamase Producing along Segments of the One Health Continuum.

Microorganisms 2020 Mar 22;8(3). Epub 2020 Mar 22.

Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.

Antimicrobial resistance (AMR) has important implications for the continued use of antibiotics to control infectious diseases in both beef cattle and humans. AMR along the One Health continuum of the beef production system is largely unknown. Here, whole genomes of presumptive extended-spectrum β-lactamase (ESBL-EC) from cattle feces ( = 40), feedlot catch basins ( = 42), surrounding streams ( = 21), a beef processing plant ( = 4), municipal sewage ( = 30), and clinical patients ( = 25) are described. Read More

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