14,473 results match your criteria bacteria colony-forming


Comparative genomics reveals an SNP potentially leading to phenotypic diversity of serovar Enteritidis.

Microb Genom 2021 May;7(5)

National Research Laboratory of Molecular Microbiology and Toxicology, Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul, Republic of Korea.

An SNP is a spontaneous genetic change having a potential to modify the functions of the original genes and to lead to phenotypic diversity of bacteria in nature. In this study, a phylogenetic analysis of serovar Enteritidis, a major food-borne pathogen, showed that eight strains of . Enteritidis isolated in South Korea, including FORC_075 and FORC_078, have almost identical genome sequences. Read More

View Article and Full-Text PDF

The Safety and Antimicrobial Properties of Stabilized Hypochlorous Acid in Acetic Acid Buffer for the Treatment of Acute Wounds-a Human Pilot Study and In Vitro Data.

Int J Low Extrem Wounds 2021 May 5:15347346211015656. Epub 2021 May 5.

SoftOx Solutions AS, Oslo, Norway.

Acute wounds may require cleansing to reduce the risk of infection. Stabilized hypochlorous acid in acetic buffer (HOCl + buffer) is a novel wound irrigation solution with antimicrobial properties. We performed a first-in-man, prospective, open-label pilot study to document preliminary safety and performance in the treatment of acute wounds. Read More

View Article and Full-Text PDF

Revisiting the inoculum effect for Streptococcus pyogenes with a hollow fibre infection model.

Eur J Clin Microbiol Infect Dis 2021 May 4. Epub 2021 May 4.

Faculty of Health and Medical Sciences, University of Western Australia, Perth, Western Australia, Australia.

Severe, invasive Streptococcus pyogenes (Strep A) infections result in greater than 500,000 deaths annually. First line treatment for such infections is benzylpenicillin, often with the addition of clindamycin, but treatment failure can occur with this regimen. This failure has been partially attributed to the inoculum effect, which presents as reduced antibiotic susceptibility during high bacterial density and plateau-phase growth. Read More

View Article and Full-Text PDF

Enzymatic Time-Temperature Indicator Prototype Developed by Immobilizing Laccase on Electrospun Fibers to Predict Lactic Acid Bacterial Growth in Milk during Storage.

Nanomaterials (Basel) 2021 Apr 29;11(5). Epub 2021 Apr 29.

Department of Food Science, National Ilan University, 1, Sec. 1, Shennong Rd., Yilan County, Yilan City 26047, Taiwan.

Laccase was immobilized on a chitosan/polyvinyl alcohol/tetraethylorthosilicate electrospun film (ceCPTL) and colored with guaiacol to obtain a laccase time-temperature indicator (TTI) prototype. The activation energy () of coloration of the prototype was 50.89-33. Read More

View Article and Full-Text PDF

Fast and Sensitive Bacteria Detection by Boronic Acid Modified Fluorescent Dendrimer.

Sensors (Basel) 2021 Apr 30;21(9). Epub 2021 Apr 30.

Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, Tokyo 102-8554, Japan.

This study reports a novel, fast, easy, and sensitive detection method for bacteria which is urgently needed to diagnose infections in their early stages. Our work presents a complex of poly(amidoamine) dendrimer modified by phenylboronic acid and labeled by a fluorescent dansyl group (Dan-B8.5-PAMAM). Read More

View Article and Full-Text PDF

Changes in growth kinetic parameters, morphology and mitotic activity of yeasts Candida guilliermondii exposed to the low-intensity waves of 51.8-GHz frequency.

Arch Microbiol 2021 May 3. Epub 2021 May 3.

Department of Biochemistry, Microbiology and Biotechnology, Yerevan State University, 1 A. Manoogyan Str, 0025, Yerevan, Armenia.

Under the influence of electromagnetic waves of millimeter range with the frequency of 51.8 GHz, changes in the morphology, growth parameters and mitotic activity of yeasts C. guilliermondii NP-4 are revealed. Read More

View Article and Full-Text PDF

Treatment of cradle cap in infants with a new cosmetic non-steroidal gel cream: Clinical, laboratory, and instrumental evaluation.

J Cosmet Dermatol 2021 Apr;20 Suppl 1:14-17

Dermatology Clinic, University of Catania, Catania, Italy.

Background: Cradle cap is a benign and self-limiting variant of seborrheic dermatitis (SD) that can be distressing for parents.

Aims: To assess by clinical/laboratory/instrumental evaluation the efficacy/tolerability of a gel cream containing piroctone olamine (antifungal), biosaccharide gum-2 (antifungal), stearyl glycyrrhetinate (anti-inflammatory), and zinc l-pyrrolidone carboxylate (zinc-PCA) (antiseborrheic) in the treatment of mild/ moderate cradle cap.

Methods: In this prospective, open-label trial, 10 infants, with mild/moderate cradle cap enrolled at the Dermatology University Clinic of Catania (Italy) used the tested gel cream twice daily for 30 days. Read More

View Article and Full-Text PDF

Quantification of biofilm produced by clinical, environment and hands' isolates Klebsiella species using colorimetric and classical methods.

J Microbiol Methods 2021 Apr 27:106231. Epub 2021 Apr 27.

Clinical Microbiology, Institute of Biomedical Sciences, Federal University of Uberlândia (UFU), Brazil.

Some species of Klebsiella, such as Klebsiella pneumoniae and Klebsiella oxytoca, are important nosocomial pathogens frequently involved in outbreaks in Neonatal Intensive Care Units (NICU) and have the ability to form a biofilm. This study aims to evaluate the biofilm production of K. pneumoniae and K. Read More

View Article and Full-Text PDF

Topical Application of Ozonated Oils for the Treatment of MRSA Skin Infection in an Animal Model of Infected Ulcer.

Biology (Basel) 2021 Apr 26;10(5). Epub 2021 Apr 26.

Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal.

Diabetic foot ulcers are a common cause of morbidity in diabetic patients. One of the main pathogens found in these ulcers is methicillin-resistant (MRSA). MRSA often carries resistance to several classes of antibiotics and their infections are becoming harder to treat. Read More

View Article and Full-Text PDF

A Rapid and Sensitive Europium Nanoparticle-Based Lateral Flow Immunoassay Combined with Recombinase Polymerase Amplification for Simultaneous Detection of Three Food-Borne Pathogens.

Int J Environ Res Public Health 2021 Apr 26;18(9). Epub 2021 Apr 26.

Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, China.

Food-borne pathogens have become an important public threat to human health. There are many kinds of pathogenic bacteria in food consumed daily. A rapid and sensitive testing method for multiple food-borne pathogens is essential. Read More

View Article and Full-Text PDF

Co-Microencapsulated Black Rice Anthocyanins and Lactic Acid Bacteria: Evidence on Powders Profile and In Vitro Digestion.

Molecules 2021 Apr 28;26(9). Epub 2021 Apr 28.

Faculty of Food Science and Engineering, Dunarea de Jos University of Galati, Romania, 111 Domneasca Street, 800201 Galati, Romania.

Two multi-functional powders, in terms of anthocyanins from black rice ( L.) and lactic acid bacteria () were obtained through co-microencapsulation into a biopolymer matrix composed of milk proteins and inulin. Two extracts were obtained using black rice flour as a raw material and hot water and ethanol as solvents. Read More

View Article and Full-Text PDF

Bacterial Microbiota of Field-Collected (Lepidoptera: Noctuidae) from Transgenic Bt and Non-Bt Cotton.

Microorganisms 2021 Apr 20;9(4). Epub 2021 Apr 20.

Department of Entomology and Plant Pathology, North Carolina State University, 3230 Ligon Street, Campus Box 7647, Raleigh, NC 27695-7647, USA.

The bollworm, (Boddie) (Lepidoptera: Noctuidae), is an important agricultural pest in U.S. cotton and is managed using transgenic hybrids that produce insecticidal proteins from the bacterium, (Bt). Read More

View Article and Full-Text PDF

Comparison of In Vitro Killing Activity of Rezafungin, Anidulafungin, Caspofungin, and Micafungin against Four Clades in RPMI-1640 in the Absence and Presence of Human Serum.

Microorganisms 2021 Apr 16;9(4). Epub 2021 Apr 16.

Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.

is an emerging and frequently multidrug-resistant pathogen against which the echinocandins are the preferred therapeutic option. We compared killing activities of anidulafungin, caspofungin, micafungin, and rezafungin against 13 isolates representing four clades (South Asian = 3; East Asian = 3; South African = 3; South American = 4, of which two were of environmental origin). Minimum inhibitory concentration MICs and killing kinetics in RPMI-1640 and RPMI-1640 plus 50% serum (50% serum) were determined. Read More

View Article and Full-Text PDF

Cultivation-Based Quantification and Identification of Bacteria at Two Hygienic Key Sides of Domestic Washing Machines.

Microorganisms 2021 Apr 23;9(5). Epub 2021 Apr 23.

Faculty of Medical and Life Sciences, Institute of Precision Medicine, Microbiology and Hygiene Group, Furtwangen University, 78054 Villingen-Schwenningen, Germany.

Detergent drawer and door seal represent important sites for microbial life in domestic washing machines. Interestingly, quantitative data on the microbial contamination of these sites is scarce. Here, 10 domestic washing machines were swab-sampled for subsequent bacterial cultivation at four different sampling sites: detergent drawer and detergent drawer chamber, as well as the top and bottom part of the rubber door seal. Read More

View Article and Full-Text PDF

Investigating the Use of Ultraviolet Light Emitting Diodes (UV-LEDs) for the Inactivation of Bacteria in Powdered Food Ingredients.

Foods 2021 Apr 8;10(4). Epub 2021 Apr 8.

Department of Biological Sciences, Munster Technological University, T12 P928 Cork, Ireland.

The addition of contaminated powdered spices and seasonings to finished products which do not undergo further processing represents a significant concern for food manufacturers. To reduce the incidence of bacterial contamination, seasoning ingredients should be subjected to a decontamination process. Ultraviolet light emitting diodes (UV-LEDs) have been suggested as an alternative to UV lamps for reducing the microbial load of foods, due to their increasing efficiency, robustness and decreasing cost. Read More

View Article and Full-Text PDF

Surface Topography, Bacterial Carrying Capacity, and the Prospect of Microbiome Manipulation in the Sea Anemone Coral Model Aiptasia.

Front Microbiol 2021 8;12:637834. Epub 2021 Apr 8.

Division of Biological and Environmental Science and Engineering, Red Sea Research Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.

Aiptasia is an emerging model organism to study cnidarian symbioses due to its taxonomic relatedness to other anthozoans such as stony corals and similarities of its microalgal and bacterial partners, complementing the existing (Hydrozoa) and (Anthozoa) model systems. Despite the availability of studies characterizing the microbiomes of several natural Aiptasia populations and laboratory strains, knowledge on basic information, such as surface topography, bacterial carrying capacity, or the prospect of microbiome manipulation is lacking. Here we address these knowledge gaps. Read More

View Article and Full-Text PDF

Clinical evaluation of the acuitas® AMR gene panel for rapid detection of bacteria and genotypic antibiotic resistance determinants.

Diagn Microbiol Infect Dis 2021 Mar 26;100(4):115383. Epub 2021 Mar 26.

OpGen, Inc., Gaithersburg, MD, USA.

Urinary tract infections are leading causes of hospital admissions. Accurate and timely diagnosis is important due to increasing morbidity and mortality from antimicrobial resistance. We evaluated a polymerase chain reaction test (Acuitas AMR Gene Panel with the Acuitas Lighthouse Software) for detection of 5 common uropathogens (Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Enterococcus faecalis) and antibiotic resistance genes directly from urine for prediction of phenotypic resistance. Read More

View Article and Full-Text PDF

Comparative Evaluation of the Efficacy of Novel Root Canal Irrigation Techniques on Reduction of Count: An Study.

J Contemp Dent Pract 2020 Dec 1;21(12):1379-1383. Epub 2020 Dec 1.

Department of Conservative Dentistry and Endodontics, Adhiprasakthi Dental College and Hospital, Tamil Nadu Dr. MGR Medical University, Melmaruvathur, Tamil Nadu, India, Phone: +91 9444792260, e-mail:

Aim And Objective: To compare the effectiveness of three irrigation systems, namely, Endovac system, Max I probe, and Navitip FX, in reduction of population from the root canal using agar diffusion method.

Materials And Methods: Sixty extracted intact human permanent maxillary anterior teeth were selected for this study. In group I, root canals were irrigated using brush covered 30-gauge NaviTipFX. Read More

View Article and Full-Text PDF
December 2020

Comparative Study of Antifungal Efficacy of Various Endodontic Irrigants with and without Clotrimazole in Extracted Teeth Inoculated with .

J Contemp Dent Pract 2020 Dec 1;21(12):1325-1330. Epub 2020 Dec 1.

Department of Oral and Maxillofacial Surgery, Narsinbhai Patel Dental College and Hospital, Sankalchand Patel University, Visnagar, Gujarat, India.

Aim And Objective: To assess the application of clotrimazole (1%) as a complementary antifungal agent along with sodium hypochlorite (5.25%), chlorhexidine gluconate (2%), and doxycycline hydrochloride (5%) against .

Materials And Methods: Seventy freshly extracted single-rooted premolars with matured apices were collected, stored, and handled according to the Occupational Safety and Health Administration (OSHA) and the Center for Disease Control and Prevention (CDC) guidelines and recommendations. Read More

View Article and Full-Text PDF
December 2020

Remineralization effectiveness of adhesive containing amorphous calcium phosphate nanoparticles on artificial initial enamel caries in a biofilm-challenged environment.

Clin Oral Investig 2021 Apr 23. Epub 2021 Apr 23.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd section, Renmin Road South, Chengdu, 610041, Sichuan, China.

Objectives: Dental caries is closely associated with acid-producing bacteria, and Streptococcus mutans is one of the primary etiological agents. Bacterial accumulation and dental demineralization lead to destruction of bonding interface, thus limiting the longevity of composite. The present study investigated remineralization effectiveness of adhesive containing nanoparticles of amorphous calcium phosphate (NACP) in a stimulated oral biofilm environment. Read More

View Article and Full-Text PDF

The Acute Host-Response of Turkeys Colonized With .

Front Vet Sci 2021 6;8:613203. Epub 2021 Apr 6.

Food Safety and Enteric Pathogens Research Unit, National Animal Disease Center, Agricultural Research Services, U.S. Department of Agriculture, Ames, IA, United States.

Consumption of contaminated poultry products is one of the main sources of human campylobacteriosis, of which subsp. () and are responsible for ~98% of the cases. In turkeys, the ceca are an important anatomical site where asymptomatically colonizes. Read More

View Article and Full-Text PDF

A quasi-experimental study on stethoscopes contamination with multidrug-resistant bacteria: Its role as a vehicle of transmission.

PLoS One 2021 22;16(4):e0250455. Epub 2021 Apr 22.

Department of Microbiology, Yeouido St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.

Stethoscopes have been suggested to be a possible vector of contact transmission. However, only a few studies have focused on the prevalence of contamination by multidrug-resistant (MDR) bacteria and effectiveness of disinfection training to reduce. This study is to investigate the burden of stethoscope contamination with nosocomial pathogens and multidrug-resistant (MDR) bacteria and to analyze habit changes in disinfection of stethoscopes among healthcare workers (HCWs) before and after education and training. Read More

View Article and Full-Text PDF

Bacterial contamination of nonsterile gloves versus hands after hand hygiene.

Am J Infect Control 2021 Apr 18. Epub 2021 Apr 18.

Department of Hospital Hygiene, Klinikum Stuttgart, Kriegsbergstraße 60, 70174 Stuttgart Germany.

Background: Nonsterile gloves (NSG) are often overused, while the emphasis should lie in hand hygiene (HH). Furthermore, improper HH leads to contamination of NSG in glove boxes. The aim of this study was to compare microbial loads on hands from health-care workers (HCW) after HH to NSG and to study the influence of position and filling level of glove boxes on contamination rates. Read More

View Article and Full-Text PDF

Reduction of Norovirus Surrogates Alone and in Association with Bacteria on Leaf Lettuce and Tomatoes During Application of Aqueous Ozone.

Food Environ Virol 2021 Apr 20. Epub 2021 Apr 20.

Department of Food Science, University of Arkansas System Division of Agriculture, 1371 West Altheimer Dr., Fayetteville, AR, 72704, USA.

Retail foodservice establishments (FSE) frequently utilize washes with sanitizing agents during fresh produce preparation. This study evaluated the efficacy of ozonated water on the inactivation of viruses, bacteria, and viruses in association with bacteria on produce surfaces. Boston bibb lettuce (BB) and cherry tomatoes were spot inoculated with viruses (murine norovirus (MNV) and MS2 bacteriophage), bacteria (Enterobacter cloacae and Bacillus cereus), or MNV associated with E. Read More

View Article and Full-Text PDF

Bioactivity Ingredients of against Microbes: Characterization by LC-MS and Activity Evaluation.

J Agric Food Chem 2021 Apr 20;69(16):4686-4696. Epub 2021 Apr 20.

Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education; Yunnan Provincial Center for Research & Development of Natural Products; School of Chemical Science and Technology, Yunnan University, Kunming 650091, P. R. China.

(Sweet) Nakai is a dual-purpose Chinese herbal medicine and functional food favored by minorities in Southwest China, and its fruits are used for the treatment of dyspepsia, dysentery, enteritis, and rheumatism inflammation. Some diseases may be related to microbial infection; however, it is not known how the fruits possess antimicrobial activity. We evaluated the antimicrobial bioctivity of different evaluation extracts of fruits by in vitro and in vivo with colony-forming unit assays, and the strongest bioactive-guided fraction was selected for column chromatography (CC), UHPLC-QTOF-MS/MS, and NMR spectroscopy to confirm the chemical constituents. Read More

View Article and Full-Text PDF

Fermentation characteristics and bacterial dynamics during Chinese sauerkraut fermentation by Lactobacillus curvatus LC-20 under varied salt concentrations reveal its potential in low-salt suan cai production.

J Biosci Bioeng 2021 Apr 14. Epub 2021 Apr 14.

College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang 150036, China. Electronic address:

Salt profoundly affects the physicochemical properties and microbial abundance of fermented foods such as suan cai, a popular traditional fermented food in China. It is vital to systematically investigate the effects of salt concentrations on fermented suan cai for high fermentation quality and large-scale production. We elucidated the effects of salt concentrations on Lactobacillus curvatus (LC-20) and suan cai during fermentation, and found that salt (0-1%) favoured an increase in LC-20 growth and a decrease in pH (salt: 0-2%). Read More

View Article and Full-Text PDF

Resistance-guided isolation and characterization of antibiotic-producing bacteria from river sediments.

BMC Microbiol 2021 Apr 17;21(1):116. Epub 2021 Apr 17.

Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh.

Background: To tackle the problem of antibiotic resistance, an extensive search for novel antibiotics is one of the top research priorities. Around 60% of the antibiotics used today were obtained from the genus Streptomyces. The river sediments of Bangladesh are still an unexplored source for antibiotic-producing bacteria (APB). Read More

View Article and Full-Text PDF

ANTIBIOFILM ACTIVITY OF DICLOFENAC AND ANTIBIOTIC SOLUTIONS IN ENDODONTIC THERAPY.

J Endod 2021 Apr 14. Epub 2021 Apr 14.

Department of Stomatology, School of Dentistry, University of Granada, Granada, Spain.

Introduction: The aim of this study was to compare the antibiofilm effects of a triple antibiotic solution (TAs), a double antibiotic solution (DAs), and 5%, 2.5% and 1.25% Diclofenac solutions (DCs) against Enteroccocus faecalis biofilm. Read More

View Article and Full-Text PDF

Antivirulence activity and in vivo efficacy of a thiazole derivative against candidiasis.

J Mycol Med 2021 Apr 6;31(2):101134. Epub 2021 Apr 6.

Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, PO Box 486, 31270-901 Belo Horizonte, Minas Gerais, Brazil. Electronic address:

Candida albicans is a pathogen equipped with a variety of commensal and virulence traits that help it colonize the microbiota and invade host tissue during infection. In this study, we investigated the potential anticandidal activity of 3-[2-(4-(4-methoxyphenyl)thiazol-2-yl)hydrazino)]butan-1-ol (MT), a thiazolylhydrazone compound synthesized by our group, and identified it as a promising antifungal agent. The activity of MT was evaluated in vitro and in vivo against C. Read More

View Article and Full-Text PDF

β-Glycyrrhetinic acid inhibits the bacterial growth and biofilm formation by supragingival plaque commensals.

Microbiol Immunol 2021 Apr 16. Epub 2021 Apr 16.

Department of Oral Microbiology, Faculty of Dentistry, Matsumoto Dental University, Shiojiri, Nagano, Japan.

β-Glycyrrhetinic acid (BGA) is a natural antibacterial agent. Previous studies reported that BGA has antibacterial effects against several bacteria. In this study, we evaluated the effects of BGA on the regulation of supragingival plaque bacteria. Read More

View Article and Full-Text PDF