Sci Total Environ 2021 Aug 18;780:146519. Epub 2021 Mar 18.
Center for Applied NanoBioscience and Medicine, The University of Arizona, College of Medicine, 475 N Fifth Street, AZ 85004, Phoenix, USA; Department of Basic Medical Sciences, The University of Arizona, College of Medicine, 475 N 5th St., Phoenix, AZ 85004, USA; School of Pharmaceutical Sciences, University of Geneva, 1 rue Michel Servet, 1211, Geneva 4, Switzerland. Electronic address:
The purpose of this research is to evaluate the supercritical carbon dioxide (scCO) sterilization-based NovaClean process for decontamination and reprocessing of personal protective equipment (PPE) such as surgical masks, cloth masks, and N95 respirators. Preliminarily, Bacillus atrophaeus were inoculated into different environments (dry, hydrated, and saliva) to imitate coughing and sneezing and serve as a "worst-case" regarding challenged PPE. The inactivation of the microbes by scCO sterilization with NovaKill or HO sterilant was investigated as a function of exposure times ranging from 5 to 90 min with a goal of elucidating possible mechanisms. Read More