295 results match your criteria bacillus atrophaeus

Effect of Metabolites Produced by Bacillus atrophaeus and Brevibacterium frigoritolerans Strains on Postharvest Biocontrol of Alternaria alternata in Tomato (Solanum lycopersicum L.).

Biocontrol Sci 2021 ;26(2):67-74

Laboratorio Integral de Investigación en Alimentos/Departamento de Química y Bioquímica, Tecnológico Nacional de México-Instituto de Tepic.

In the present study, the antifungal activity of metabolites produced by Bacillus atrophaeus B5 and a new Brevibacterium strain against Alternaria alternata was evaluated. Assays in vitro and in vivo on tomato fruit during postharvest were made. Based on the 16S rDNA gene sequence analysis, the new strain (strain B7) was identified as Brevibacterium frigoritolerans. Read More

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January 2021

Nematicidal Volatiles from GBSC56 Promote Growth and Stimulate Induced Systemic Resistance in Tomato against .

Int J Mol Sci 2021 May 10;22(9). Epub 2021 May 10.

Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.

volatiles to control plant nematodes is a topic of great interest among researchers due to its safe and environmentally friendly nature. strain GBSC56 isolated from the Tibet region of China showed high nematicidal activity against , with 90% mortality as compared with control in a partition plate experiment. Pure volatiles produced by GBSC56 were identified through gas chromatography and mass spectrometry (GC-MS). Read More

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Levels and Characteristics of mRNAs in Spores of Firmicute Species.

J Bacteriol 2021 May 10. Epub 2021 May 10.

Departments of Molecular Biology and Biophysics, Medicine and Pediatrics UConn Health, Farmington, CT 06030-3305 USA

Spores of Firmicute species contain 100s of mRNAs, whose major function in is to provide ribonucleotides for new RNA synthesis when spores germinate. To determine if this is a general phenomenon, RNA was isolated from spores of multiple Firmicute species and relative mRNA levels determined by RNA-seq. Determination of RNA levels in single spores allowed calculation of RNA nt/spore, and assuming mRNA is 3% of spore RNA indicated that only ∼6% of spore mRNAs were present at > 1/spore. Read More

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Managing the Bacterial Contamination Risk in an Axenic Mice Animal Facility.

Can J Microbiol 2021 Apr 12. Epub 2021 Apr 12.

Institut universitaire de cardiologie et de pneumologie de Quebec, 55973, Research center, Quebec, Quebec, Canada.

A gap exists between good laboratory practises with axenic animals and procedures applied. This work aimed at choosing the appropriate disinfectant between sodium dichloroisocyanurate (MB-10) and potassium peroxymonosulfate (Virkon) disinfectants and to adjust the soaking time of the material used with ISOcage biosafety stations. Another aim was to compare the microbial load on cage systems hosting mice since two weeks in axenic (AR) rooms and typical specific-pathogen-free (SPF) non-axenic rooms (NAR) to identify resistant microorganisms targeted for longer soaking disinfection as well as evaluate microbial concentration reduction procedures in AR. Read More

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Synergistic Effects of Nisin, Lysozyme, Lactic Acid, and Citricidal for Enhancing Pressure-Based Inactivation of , , and Endospores.

Microorganisms 2021 Mar 21;9(3). Epub 2021 Mar 21.

Public Health Microbiology Laboratory, Tennessee State University, Nashville, TN 37209, USA.

The inactivation of bacterial endospores continues to be the main curtailment for further adoption of high-pressure processing in intrastate, interstate, and global food commerce. The current study investigated the effects of elevated hydrostatic pressure for the inactivation of endospore suspension of three indicator spore-forming bacteria of concern to the food industry. Additionally, the effects of four bacteriocin/bactericidal compounds were studied for augmenting the decontamination efficacy of the treatment. Read More

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Evaluation of supercritical CO sterilization efficacy for sanitizing personal protective equipment from the coronavirus SARS-CoV-2.

Sci Total Environ 2021 Aug 18;780:146519. Epub 2021 Mar 18.

Center for Applied NanoBioscience and Medicine, The University of Arizona, College of Medicine, 475 N Fifth Street, AZ 85004, Phoenix, USA; Department of Basic Medical Sciences, The University of Arizona, College of Medicine, 475 N 5th St., Phoenix, AZ 85004, USA; School of Pharmaceutical Sciences, University of Geneva, 1 rue Michel Servet, 1211, Geneva 4, Switzerland. Electronic address:

The purpose of this research is to evaluate the supercritical carbon dioxide (scCO) sterilization-based NovaClean process for decontamination and reprocessing of personal protective equipment (PPE) such as surgical masks, cloth masks, and N95 respirators. Preliminarily, Bacillus atrophaeus were inoculated into different environments (dry, hydrated, and saliva) to imitate coughing and sneezing and serve as a "worst-case" regarding challenged PPE. The inactivation of the microbes by scCO sterilization with NovaKill or HO sterilant was investigated as a function of exposure times ranging from 5 to 90 min with a goal of elucidating possible mechanisms. Read More

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Lipopeptide production by Bacillus atrophaeus strain B44 and its biocontrol efficacy against cotton rhizoctoniosis.

Biotechnol Lett 2021 Jun 18;43(6):1183-1193. Epub 2021 Mar 18.

Key Laboratory at Universities of Xinjiang Uygur Autonomous Region for Oasis Agricultural Pest Management and Plant Protection Resource Utilization/College of Agriculture, Shihezi University, Shihezi, 832003, China.

Objectives: An assay was conducted to show the comparisons the effects of nine metal ions on antagonistic metabolites (lipopeptides, siderophores and gibberellins) by Bacillus atrophaeus strain B44 using well-diffusion assays, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis, chrome azurol S plus mannitol salt agar (CAS-MSA) tests, and reversed-phase high-performance liquid chromatography (RP-HPLC) analysis. This assay is also designed to demonstrate the biocontrol efficacy of B44 against cotton rhizoctoniosis using pot culture tests.

Results: Both the lipopeptide yield and the antimicrobial activity of B44 increase with the MnSO, MgSO, CaCO, and CuSO treatments and either have no effect or decreased lipopeptide yield and antimicrobial activity with the FeSO, KHPO, KCl, KHPO and ZnSO treatments. Read More

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Toward a Test Protocol for Surface Decontamination Using a Mobile Whole-room UVGI Device.

Photochem Photobiol 2021 May 21;97(3):552-559. Epub 2021 Apr 21.

Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY.

Mobile whole-room UVGI devices are used in healthcare settings to control surface-borne pathogens. Unfortunately, no standard method comparing the efficacy of these devices is available. We accessed the effect of shadows on UVC 254 nm inactivation. Read More

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Biochemical characterization and synthetic application of aromatic L-amino acid decarboxylase from Bacillus atrophaeus.

Appl Microbiol Biotechnol 2021 Apr 13;105(7):2775-2785. Epub 2021 Mar 13.

Department of Biotechnology, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, South Korea.

Aromatic L-amino acid decarboxylases (AADCs) are ubiquitously found in higher organisms owing to their physiological role in the synthesis of neurotransmitters and alkaloids. However, bacterial AADC has not attracted much attention because of its rather limited availability and narrow substrate range. Here, we examined the biochemical properties of AADC from Bacillus atrophaeus (AADC-BA) and assessed the synthetic feasibility of the enzyme for the preparation of monoamine neurotransmitters. Read More

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Biocontrol of Cladosporium cladosporioides of mango fruit with Bacillus atrophaeus TE7 and effects on storage quality.

Curr Microbiol 2021 Feb 19;78(2):765-774. Epub 2021 Jan 19.

College of Food Science, Hainan University, Haikou, 570228, China.

The purpose of this study was to evaluate the control effect of Bacillus atrophaeus TE7 on Cladosporium cladosporioides of mango fruit and how it effects quality attributes during 'Tainong' mango fruit storage. The results showed that strain TE7 had inhibition ability with the biocontrol efficacy of 85.56%. Read More

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February 2021

Differentiation of bacterial spores via 2D-IR spectroscopy.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Mar 14;249:119319. Epub 2020 Dec 14.

Department of Chemistry and York Biomedical Research Institute, University of York, Heslington, York YO10 5DD, UK.

Ultrafast 2D-IR spectroscopy is a powerful tool for understanding the spectroscopy and dynamics of biological molecules in the solution phase. A number of recent studies have begun to explore the utility of the information-rich 2D-IR spectra for analytical applications. Here, we report the application of ultrafast 2D-IR spectroscopy for the detection and classification of bacterial spores. Read More

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Decontamination of soil contaminated at the surface with Bacillus anthracis spores using dry thermal treatment.

J Environ Manage 2021 Feb 7;280:111684. Epub 2020 Dec 7.

United States Environmental Protection Agency, Office of Research and Development, Research Triangle Park, NC, USA.

In the event of a large, aerosol release of Bacillus anthracis spores in a major metropolitan area, soils and other outdoor materials may become contaminated with the biological agent. A study was conducted to assess the in-situ remediation of soil using a dry thermal treatment approach to inactivate a B. anthracis spore surrogate inoculated into soil samples. Read More

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February 2021

Ultraviolet Box: An Innovative In-house Use of Ultraviolet Irradiation for Conservation of Respirators in COVID-19 Pandemic.

Indian J Crit Care Med 2020 Aug;24(8):713-715

Department of Pulmonary and Critical Care Medicine, Pt Bhagwat Dayal Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India.

Aim: To use ultraviolet (UV) radiations in an indigenous method for sterilization of respirators for reuse during COVID-19 outbreak.

Background: COVID-19 outbreak has infected more than 200 countries. In India, till now, more than 100,000 cases have been reported. Read More

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Potential Plant Growth-Promoting Bacteria with Heavy Metal Resistance.

Derya Efe

Curr Microbiol 2020 Dec 22;77(12):3861-3868. Epub 2020 Sep 22.

Department of Medicinal and Aromatic Plants, Espiye Vocational School, Giresun University, 28000, Giresun, Turkey.

Plant growth-promoting (PGP) bacteria commonly have many strategies to cope with heavy metal toxicity. Heavy metal-resistant PGP bacteria can be used to improve the growth of plants in heavy metal contaminated soils. In this study, the soil samples were collected from the lead-zinc mineral deposits in Gümüşhane Province, Turkey. Read More

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December 2020

Field evaluation of biocontrol agents against black-foot and Petri diseases of grapevine.

Pest Manag Sci 2021 Feb 12;77(2):697-708. Epub 2020 Sep 12.

Instituto de Ciencias de la Vid y del Vino (ICVV), Consejo Superior de Investigaciones Científicas, Universidad de la Rioja, Gobierno de La Rioja, Logroño, Spain.

Background: Black-foot and Petri diseases are the main fungal diseases associated with young grapevine decline. Two field experiments were established to evaluate the preventive effect of two potential biocontrol agents (BCAs), that is Streptomyces sp. E1 + R4 and Pythium oligandrum Po37, and three BCA-commercial products containing Trichoderma atroviride SC1, Trichoderma koningii TK7 and Pseudomonas fluorescens + Bacillus atrophaeus on fungal infection in grafted plants and plant growth parameters. Read More

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February 2021

Low-concentration hydrogen peroxide decontamination for spore contamination in buildings.

Remediation (N Y) 2019 Dec;30(1):47-56

Homeland Security Department, Jacobs Technology Inc., Durham, North Carolina.

Remediation and recovery efforts after a release of (anthrax) spores may be difficult and costly. In addition, response and recovery technologies may be focused on critical resources, leaving the small business or homeowner without remediation options. This study evaluates the efficacy of relatively low levels of hydrogen peroxide vapor (HPV) delivered from off-the-shelf equipment for the inactivation of spores within an indoor environment. Read More

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December 2019

Optimization of immobilization conditions of Bacillus atrophaeus FSHM2 lipase on maleic copolymer coated amine-modified graphene oxide nanosheets and its application for valeric acid esterification.

Int J Biol Macromol 2020 Nov 16;162:1790-1806. Epub 2020 Aug 16.

Pharmaceutical Sciences and Cosmetic Products Research Center, Kerman University of Medical Sciences, Kerman, Iran; Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran. Electronic address:

The thermoalkalophilic lipase from Bacillus atrophaeus (BaL) was immobilized onto amine-functionalized graphene oxide nanosheets coated with the poly (maleic anhydride-alt-1-octadecene) copolymer (GO-NH-PMAO) and activated with glutaraldehyde as spacer arm through interfacial activation and subsequent multipoint covalent attachment. Experimental design method was applied for optimization of immobilization conditions including GO-NH-PMAO concentration, buffer concentration, pH, sonication time, enzyme concentration, glutaraldehyde concentration, time, and temperature. The optimum specific activity of the immobilized BaL (105. Read More

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November 2020

Bioactivity of Bac70 Produced by Strain DDBCC70.

Avicenna J Med Biotechnol 2020 Jul-Sep;12(3):186-193

Department of Biotechnology, Faculty of Biotechnology, Semnan University, Semnan, Iran.

Background: Recently, using antibacterial peptides has been considered as a strategy to manage the worldwide antibiotic-resistance crisis. Screening of Dasht-Desert Bacterial Culture Collection (DDBCC) for bacteriocin or bacteriocin-like producer was aimed in this study to introduce native antibacterial agent(s).

Methods: In this study, 170 isolates were examined by the cross-streak method against G+ and G- indicators. Read More

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Profiling for Bioactive Peptides and Volatiles of Plant Growth Promoting Strains of the Complex of Industrial Relevance.

Front Microbiol 2020 30;11:1432. Epub 2020 Jun 30.

ABiTEP GmbH, Berlin, Germany.

Plant growth promoting rhizobacteria attain increasing importance in agriculture as biofertilizers and biocontrol agents. These properties significantly depend on the formation of bioactive compounds produced by such organisms. In our work we investigated the biosynthetic potential of 13 industrially important strains of the complex by mass spectrometric methodology. Read More

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Inactivation of Bacillus anthracis and Bacillus atrophaeus spores on different surfaces with ultraviolet light produced with a low-pressure mercury vapor lamp or light emitting diodes.

J Appl Microbiol 2020 Jul 21. Epub 2020 Jul 21.

U.S. Army Chemical Biological Center, Aberdeen Proving Ground, MD, USA.

Aims: To obtain quantitative efficacy data of two ultraviolet light (UVC) technologies for surface inactivation of Bacillus anthracis Ames and Bacillus atrophaeus spores.

Methods And Results: Spores were deposited onto test coupons and controls of four different materials, via liquid suspension or aerosol deposition. The test coupons were then exposed to UVC light from either a low-pressure mercury vapor lamp or a system comprised of light emitting diodes, with a range of dosages. Read More

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Microbial Barrier Properties of Cotton Fabric-Influence of Weave Architecture.

Polymers (Basel) 2020 Jul 15;12(7). Epub 2020 Jul 15.

Faculty of Natural Sciences and Engineering, University of Ljubljana, Aškerčeva 12, 1000 Ljubljana, Slovenia.

The subject of the paper focuses on the effect of weave architecture on microbial barrier properties of woven fabrics or more precisely on identifying crucial elements of weave architecture that dominantly influence bacteria penetration in dry condition. For that purpose, 12 samples of cotton fabrics were woven and examined. In their structure, all samples had the same yarns (36 tex) in warp and weft, same densities of warp (24 yarns/cm), two weft densities (24 and 20 yarns/cm) and six different basic weave structures. Read More

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Effect of Sporulation Conditions Following Submerged Cultivation on the Resistance of Spores against Inactivation by HO.

Molecules 2020 Jun 30;25(13). Epub 2020 Jun 30.

Chair of Food and Bioprocess Engineering, Technical University of Munich, 85354 Freising, Germany.

The resistance formation of spores in general and of in particular has long been the focus of science in the bio-defense, pharmaceutical and food industries. In the food industry, it is used as a biological indicator (BI) for the evaluation of the inactivation effects of hydrogen peroxide in processing and end packaging lines' sterilization. Defined BI resistances are critical to avoid false positive and negative tests, which are salient problems due to the variable resistance of currently available commercial BIs. Read More

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Effect of peracetic acid on ascospore-forming molds and test microorganisms used for bio-validations of sanitizing processes in food plants.

Int J Food Microbiol 2020 Nov 25;332:108772. Epub 2020 Jun 25.

Stazione Sperimentale per l'Industria delle Conserve Alimentari, SSICA, Viale F. Tanara, 31/A, 43121 Parma, Italy. Electronic address:

Industrial sterilization of packaging and filling machineries by peracetic acid (PAA) is a widespread practice. In our study we assessed the resistance to PAA of three ascospore-forming molds (Chaetomium globosum ATCC 6205; Talaromyces bacillisporus SSICA 10915; Aspergillus hiratsukae SSICA 3913) compared to that of Aspergillus brasiliensis ATCC 16404 and Bacillus atrophaeus DSM 675, that are currently used as test microorganisms during industrial bio-validations of food packaging and machineries. Tests were carried out at 40 °C using 1,000 mg/l of PAA, with or without a supporting material (aluminium, tin-plate, PET). Read More

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November 2020

Survival of the Halophilic Archaeon Halovarius luteus after Desiccation, Simulated Martian UV Radiation and Vacuum in Comparison to Bacillus atrophaeus.

Orig Life Evol Biosph 2020 Dec 2;50(3-4):157-173. Epub 2020 Jul 2.

Amsterdam UMC location VUmc, and Academic Centre for Dentistry Amsterdam (ACTA) Department of Oral and Maxillofacial Surgery/Pathology, Amsterdam Movement Sciences, Vrije Universiteit Amsterdam, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands.

Extraterrestrial environments influence the biochemistry of organisms through a variety of factors, including high levels of radiation and vacuum, temperature extremes and a lack of water and nutrients. A wide variety of terrestrial microorganisms, including those counted amongst the most ancient inhabitants of Earth, can cope with high levels of salinity, extreme temperatures, desiccation and high levels of radiation. Key among these are the haloarchaea, considered particularly relevant for astrobiological studies due to their ability to thrive in hypersaline environments. Read More

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December 2020

Comparison of surface sampling methods for an extended duration outdoor biological contamination study.

Environ Monit Assess 2020 Jun 24;192(7):455. Epub 2020 Jun 24.

Office of Research and Development, Center for Environmental Solutions and Emergency Response, US EPA, Cincinnati, OH, USA.

Bacillus anthracis, the causative agent for anthrax, is a dangerous pathogen to humans and has a history as a bioterrorism agent. While sampling methods have been developed and evaluated for characterizing and clearing contaminated indoor sites, the performance of these sampling methods is unknown for use in outdoor environments. This paper presents surface sampling data for Bacillus atrophaeus spores, a surrogate for B. Read More

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A surface loop modulates activity of the Bacillus class D β-lactamases.

J Struct Biol 2020 08 5;211(2):107544. Epub 2020 Jun 5.

Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, USA. Electronic address:

The expression of β-lactamases is a major mechanism of bacterial resistance to the β-lactam antibiotics. Four molecular classes of β-lactamases have been described (A, B, C and D), however until recently the class D enzymes were thought to exist only in Gram-negative bacteria. In the last few years, class D enzymes have been discovered in several species of Gram-positive microorganisms, such as Bacillus and Clostridia, and an investigation of their kinetic and structural properties has begun in earnest. Read More

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Endophytes from Gnetum gnemon L. can protect seedlings against the infection of phytopathogenic bacterium Ralstonia solanacearum as well as promote plant growth in tomato.

Microbiol Res 2020 Sep 16;238:126503. Epub 2020 May 16.

Department of Botany, Gauhati University, Gopinath Bordoloi Nagar, Jalukbari, Assam, 781014, India. Electronic address:

Endophytes are beneficial plant microbes which help the plants by producing various plant growth promoting substances and also by acting as biocontrol agents against various plant pathogens. In the present study, evaluation of endophytic bacteria isolated from Gnetum gnemon, an ethnomedicinal plant was carried out for their plant growth promoting (PGP) activity and antagonistic potential against bacterial wilt pathogen Ralstonia solanacearum. Initially a total of 40 endophytic bacteria were isolated which were clustered into 13 groups based on RFLP and BOX-PCR fingerprinting. Read More

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September 2020

High-Efficiency Secretion and Directed Evolution of Chitinase BcChiA1 in for the Conversion of Chitinaceous Wastes Into Chitooligosaccharides.

Front Bioeng Biotechnol 2020 7;8:432. Epub 2020 May 7.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.

Limitations of enzyme production and activity pose a challenge for efficient degradation of chitinaceous wastes. To solve this problem, we engineered a system for high-yielding extracellular secretion of chitinase A1 from (BcChiA1) in . Furthermore, an innovative chitinase high-throughput screening method based on colloidal chitin stained with Remazol Brilliant Blue R (CC-RBB) was established and used to identify three mutants with improved chitinase activity: Y10A/R301A/E327A (Mu1), Y10A/D81A/E327A (Mu2), and F38A/K88A/R301A (Mu3). Read More

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Spore inactivation on solid surfaces by vaporized hydrogen peroxide-Influence of carrier material surface properties.

J Food Sci 2020 May 6;85(5):1536-1541. Epub 2020 Apr 6.

Chair of Food and Bioprocess Engineering, Technical University of Munich, Weihenstephaner Berg 1, Freising, DE, Germany.

The aim of this study was to investigate the influence of surface hydrophobicity and roughness of carrier materials on the inactivation of bacterial spores with gaseous hydrogen peroxide whereas condensate formation is prevented. Spores of Bacillus subtilis and Bacillus atrophaeus were applied either as single spore culture or as a mixed spore population to simulate natural contamination with microorganisms of different characteristics. Inactivation with gaseous hydrogen peroxide was carried out at 5200 ppm hydrogen peroxide without condensate formation. Read More

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Influence of Washing and Sterilization on Properties of Polyurethane Coated Fabrics Used in Surgery and for Wrapping Sterile Items.

Polymers (Basel) 2020 Mar 12;12(3). Epub 2020 Mar 12.

Textile Department, Faculty of Technology, University of Nis, Bulevar Oslobodjenja 124, 16000 Leskovac, Serbia.

The objective of this work was to determine the influence of washing and sterilization under real hospital conditions on properties of microbial barrier offered by polyurethane coated fabrics used in surgery and for wrapping sterile items. Emphasis was put on the change of surface polyurethane coating by using FTIR analysis. The permeability and durability of the microbial barrier were determined after 0, 10, and 20 washing and sterilization procedures according to previously developed methods. Read More

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