3,162 results match your criteria avium subsp

Application of Volatilome Analysis to the Diagnosis of Mycobacteria Infection in Livestock.

Front Vet Sci 2021 24;8:635155. Epub 2021 May 24.

Department of Anatomy and Comparative Pathology and Toxicology, International Agrifood Campus of Excellence (ceiA3), University of Córdoba, Córdoba, Spain.

Volatile organic compounds (VOCs) are small molecular mass metabolites which compose the volatilome, whose analysis has been widely employed in different areas. This innovative approach has emerged in research as a diagnostic alternative to different diseases in human and veterinary medicine, which still present constraints regarding analytical and diagnostic sensitivity. Such is the case of the infection by mycobacteria responsible for tuberculosis and paratuberculosis in livestock. Read More

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Effect of the deletion of lprG and p55 genes in the K10 strain of Mycobacterium avium subspecies paratuberculosis.

Res Vet Sci 2021 May 28;138:1-10. Epub 2021 May 28.

Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTA-CONICET, Hurlingham, Buenos Aires 1686, Argentina.

The lprG-p55 operon of Mycobacterium tuberculosis, M. bovis and M. avium strain D4ER has been identified as a virulence factor involved in the transport of toxic compounds. Read More

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Development of a reference standard for the detection and quantification of Mycobacterium avium subsp. paratuberculosis by quantitative PCR.

Sci Rep 2021 Jun 2;11(1):11622. Epub 2021 Jun 2.

Department of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, Brno, Czech Republic.

Quantitative PCR (qPCR) has become a frequently employed direct method for the detection and quantification of Mycobacterium avium subsp. paratuberculosis (MAP). The quantity of MAP determined by qPCR, however, may be affected by the type of qPCR quantification standard used (PCR product, plasmid, genomic DNA) and the way in which standard DNA quantity is determined (absorbance, fluorescence). Read More

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Detection of Subspecies in Pooled Fecal Samples by Fecal Culture and Real-Time PCR in Relation to Bacterial Density.

Animals (Basel) 2021 May 29;11(6). Epub 2021 May 29.

Animal Health Service, Thuringian Animal Diseases Fund, Victor-Goerttler-Straße 4, 07745 Jena, Germany.

Within paratuberculosis control programs subsp. (MAP)-infected herds have to be detected with minimum effort but with sufficient reliability. We aimed to evaluate a combination of random sampling (RS) and pooling for the detection of MAP-infected herds, simulating repeated RS in imitated dairy herds (within-herd prevalence 1. Read More

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Bovine Intelectin 2 Expression as a Biomarker of Paratuberculosis Disease Progression.

Animals (Basel) 2021 May 12;11(5). Epub 2021 May 12.

Center for Animal Biotechnology, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), 33394 Deva, Spain.

Paratuberculosis (PTB), a chronic granulomatous enteritis caused by   subsp. (MAP), is responsible for important economic losses in the dairy industry. Our previous RNA-sequencing (RNA-Seq) analysis showed that bovine intelectin 2 (ITLN2) precursor gene was overexpressed in ileocecal valve (ICV) samples of animals with focal (log2 fold-change = 10. Read More

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Early Detection of subsp. Infected Cattle: Use of Experimental Johnins and Innovative Interferon-Gamma Test Interpretative Criteria.

Front Vet Sci 2021 14;8:638890. Epub 2021 May 14.

Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati", Perugia, Italy.

Paratuberculosis (PTB), also known as Johne's disease, is a chronic proliferative enteritis of ruminants caused by subsp. (MAP). To date, PTB diagnosis, based on serology, fecal culture, and real-time polymerase chain reaction, has identified animals in advanced stages of infection. Read More

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Hematologic values in domestic pigeons naturally infected with Mycobacterium avium subsp. avium.

Vet Clin Pathol 2021 May 27. Epub 2021 May 27.

Razi Vaccine and Serum Research Institute, Karaj, Iran.

Avian mycobacteriosis is an important disease of birds and is most often caused by Mycobacterium avium or Mycobacterium genavense. However, little information on the hematologic changes associated with this infectious disease in pigeons has been published. The aim of this investigation was to compare the hematologic parameters of domestic pigeons (Columba livia var. Read More

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Whole-Genome Analysis of subsp. IS-.

Front Microbiol 2021 10;12:660002. Epub 2021 May 10.

INRAE, ISP, Université de Tours, Nouzilly, France.

subsp. () is the etiological agent of Johne's disease in ruminants. The IS insertion sequence (IS) has been used widely as an epidemiological marker and target for PCR diagnosis. Read More

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Comparative cellular immune responses in calves after infection with Mycobacterium avium subsp. paratuberculosis, M. avium subsp. avium, M. kansasii and M. bovis.

Vet Immunol Immunopathol 2021 Jul 19;237:110268. Epub 2021 May 19.

USDA-ARS, National Animal Disease Center, Ames, IA 50010, United States.

In the present study, calves were infected with Mycobacterium avium subsp. paratuberculosis (MAP), Mycobacterium avium subsp. avium (M. Read More

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Mycobacterium avium subsp. hominissuis (MAH) Microaggregate induction of host innate immunity is linked to biofilm formation.

Microb Pathog 2021 May 18;157:104977. Epub 2021 May 18.

Department of Biomedical Sciences, Carlson College of Veterinary Medicine, Oregon State University, Corvallis, OR, USA; Department of Microbiology, College of Science, Oregon State University, Corvallis, OR, USA. Electronic address:

Bacterial aggregation is a strategy employed by many pathogens to establish infection. Mycobacterium avium subsp. hominissuis (MAH) undergoes a phenotypic change, microaggregation, when exposed to the respiratory epithelium. Read More

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Early response of monocyte-derived macrophages from vaccinated and non-vaccinated goats against in vitro infection with Mycobacterium avium subsp. paratuberculosis.

Vet Res 2021 May 12;52(1):69. Epub 2021 May 12.

Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, Campus de Vegazana s/n, 24007, León, Spain.

Paratuberculosis is a disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). Vaccination is the most cost-effective control method. Read More

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Engineering Synthetic Lipopeptide Antigen for Specific Detection of subsp. Infection.

Front Vet Sci 2021 23;8:637841. Epub 2021 Apr 23.

INRAE, Université de Tours, ISP, Nouzilly, France.

Unlike other MAC members, subsp. (MAP) does not produce glycopeptidolipids (GPL) on the surface of the cell wall but a lipopentapeptide called L5P (also termed Lipopeptide-I or Para-LP-01) characterized in C-type (bovine) strains. This lipopeptide antigen contains a pentapeptide core, D-Phenylalanine-N-methyl-L-Valine-L-Isoleucine-L-Phenylalanine-L-Alanine, in which the N-terminal D-Phenylalanine is amido-linked with a fatty acid (C18-C20). Read More

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Reduced tissue colonization of Mycobacterium avium subsp. paratuberculosis in neonatal calves vaccinated with a cocktail of recombinant proteins.

Vaccine 2021 05 7;39(23):3131-3140. Epub 2021 May 7.

USDA-ARS, National Animal Disease Center, Ames, IA 50010, United States.

An increasing prevalence of paratuberculosis supports the need for new efficacious vaccines as an essential management tool. Two separate studies were performed in neonatal calves to evaluate the effectiveness of pooled recombinant Mycobacterium avium subsp. paratuberculosis (MAP) proteins (MAP1087, MAP1204, MAP1272c, MAP2077c) as a potential vaccine. Read More

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MAP, Johne's disease and the microbiome; current knowledge and future considerations.

Anim Microbiome 2021 May 7;3(1):34. Epub 2021 May 7.

Cork Institute of Technology, Bishopstown, Co. Cork, Ireland.

Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease in ruminants. As an infectious disease that causes reduced milk yields, effects fertility and, eventually, the loss of the animal, it is a huge financial burden for associated industries. Read More

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Complete Genome Sequence of Mycobacterium avium subsp. Strain 42-13-1, Isolated in Japan.

Microbiol Resour Announc 2021 Apr 15;10(15). Epub 2021 Apr 15.

National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan.

Here, we report the complete genome sequence of subsp. strain 42-13-1, isolated from cattle presenting with chronic diarrhea caused by Johne's disease in Japan, which was assembled via long- and short-read hybrid assembly. Read More

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Prediction of Johne's disease state based on quantification of T cell markers and their interaction with macrophages in the bovine intestine.

Vet Res 2021 Apr 13;52(1):55. Epub 2021 Apr 13.

USDA-Agricultural Research Service (ARS), National Animal Disease Center, Ames, IA, USA.

Cell-mediated immune responses to Mycobacterium avium subsp. paratuberculosis (MAP) are regulated by various types of T lymphocytes. The aim of this study was to quantitate T cell subsets in the mid-ileum of cows naturally infected with MAP to identify differences during different stages of infection, and to determine whether these subsets could be used as predictors of disease state. Read More

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Comparison of Susceptibility of Delafloxacin with Ciprofloxacin, Moxifloxacin, and other Comparator Antimicrobials Against Isolates of Nontuberculous Mycobacteria.

Antimicrob Agents Chemother 2021 Apr 12. Epub 2021 Apr 12.

The University of Texas Health Science Center at Tyler, Mycobacteria/Nocardia Laboratory, 11937 U.S. Hwy. 271, Tyler Texas 75708.

Nontuberculous mycobacteria (NTM) infections are increasing globally. complex (MAC) and complex are the most commonly reported NTM. Oral treatment options are limited, especially for the We tested delafloxacin, a new oral fluoroquinolone, against 131 isolates of NTM. Read More

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Interferon-γ Response of subsp. Infected Goats to Recombinant and Synthetic Mycobacterial Antigens.

Front Vet Sci 2021 26;8:645251. Epub 2021 Mar 26.

Animal and Plant Health Agency, Addlestone, United Kingdom.

Despite its potential for early diagnosis of subsp. (MAP) infection, the IFN-γ release assay is not used routinely, because of low specificity of the established crude antigen preparation Johnin (PPDj). Limited data are available assessing the potential of MAP-derived protein and lipopeptide antigens to replace PPDj in assays for goats, while cattle and sheep have been studied more extensively. Read More

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J Wildl Dis 2021 04;57(2):321-326

Programa de Ganadería, Campus Montecillo, Colegio de Postgraduados (ColPos), Km 36.5 Carretera México-Texcoco, Montecillo, Texcoco, Mexico, 56230.

Paratuberculosis (PTB) is a disease that affects cattle (Bos taurus), goats (Capra aegagrus hircus), sheep (Ovis aries), and wild animals, such as white-tailed deer (Odocoileus virginianus), since all ruminants are susceptible. The causal agent is Mycobacterium avium subsp. paratuberculosis (MAP). Read More

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Bovine Neutrophils Release Extracellular Traps and Cooperate With Macrophages in subsp. clearance .

Front Immunol 2021 17;12:645304. Epub 2021 Mar 17.

Animal Health Department, NEIKER-Basque Institute for Agricultural Research and Development-Basque Research and Technology Allianca (BRTA), Derio, Spain.

subsp. (Map) is the underlying pathogen causing bovine paratuberculosis (PTB), an enteric granulomatous disease that mainly affects ruminants and for which an effective treatment is needed. Macrophages are the primary target cells for Map, which survives and replicates intracellularly by inhibiting phagosome maturation. Read More

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Preparation of immunomagnetic beads coupled with a rhodamine hydrazine immunosensor for the detection of Mycobacterium avium subspecies paratuberculosis in bovine feces, milk, and colostrum.

J Dairy Sci 2021 Jun 2;104(6):6944-6960. Epub 2021 Apr 2.

Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana-Champaign 61802.

The aim of this study was to develop and evaluate a method for detecting Mycobacterium avium ssp. paratuberculosis (MAP) bacteria in bovine fecal, milk, and colostrum samples using immunomagnetic beads (IMB) and a rhodamine hydrazone immunosensor. Immunomagnetic beads were prepared by using purified antibodies from hyperimmunized sera that were coupled to Fe nanoparticles with diethylene triamine pentaacetic acid (DTPA) or ethyl (dimethyl aminopropyl) carbodiimide (EDC)-N-hydroxy succinimide (NHS) as linkers. Read More

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Genetic Involvement of Complex in the Regulation and Manipulation of Innate Immune Functions of Host Cells.

Int J Mol Sci 2021 Mar 16;22(6). Epub 2021 Mar 16.

Department of Microbiology and Institute for Immunology and Immunological Diseases, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 03722, Korea.

complex (MAC), a collection of mycobacterial species representing nontuberculous mycobacteria, are characterized as ubiquitous and opportunistic pathogens. The incidence and prevalence of infectious diseases caused by MAC have been emerging globally due to complications in the treatment of MAC-pulmonary disease (PD) in humans and the lack of understating individual differences in genetic traits and pathogenesis of MAC species or subspecies. Despite genetically close one to another, mycobacteria species belonging to the MAC cause diseases to different host range along with a distinct spectrum of disease. Read More

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Bio-typing of Mycobacterium avium subspecies paratuberculosis isolates recovered from the Himalayan sheep and goats.

Trop Anim Health Prod 2021 Mar 31;53(2):237. Epub 2021 Mar 31.

Microbiology Lab, Animal Health Division, Central Institute for Research on Goats, Mathura, U.P., India.

Information on bio-type profile of Mycobacterium avium subspecies paratubeculosis (MAP) in sheep flocks and goat herds of Himalayan region is not reported earlier. The aim of our study was to determine the bio-type of MAP infecting livestock of this region. A total of 71 faecal samples (sheep-57, goats-14) were screened by Ziehl-Neelsen (ZN) staining and IS900 PCR, and then processed for culture on Herrold's egg yolk medium (HEYM) having mycobactin J (MJ). Read More

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Electrochemical Detection of Serum Antibodies Against Subspecies .

Front Vet Sci 2021 9;8:642833. Epub 2021 Mar 9.

Department of Forestry, Wildlife and Fisheries, University of Tennessee Institute of Agriculture, Knoxville, Knoxville, TN, United States.

subsp. (MAP) causes a chronic inflammatory intestinal disease, called Johne's disease (JD) in many ruminants. In the dairy industry, JD is responsible for significant economic losses due to decreased milk production and premature culling of infected animals. Read More

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Molecular characterisation of Mycobacterium avium subsp. paratuberculosis in Australia.

BMC Microbiol 2021 Apr 1;21(1):101. Epub 2021 Apr 1.

Agriculture Victoria, AgriBio, La Trobe University, Bundoora, Victoria, Australia.

Background: Mycobacterium avium subsp. paratuberculosis (Map) causes Johne's disease (JD), a chronic enteritis widespread in ruminants, resulting in substantial economic losses, especially to the dairy industry. Understanding the genetic diversity of Map in Australia will assist epidemiological studies for tracking disease transmission and identify subtype characteristics for use in development of improved diagnostic typing methods. Read More

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Association of TLR2 haplotypes encoding Q650 with reduced susceptibility to ovine Johne's disease in Turkish sheep.

Sci Rep 2021 Mar 29;11(1):7088. Epub 2021 Mar 29.

USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE, 68933, USA.

Ovine Johne's disease (OJD) is caused by Mycobacterium avium subsp. paratuberculosis (MAP) and carries a potential zoonotic risk for humans. Selective breeding strategies for reduced OJD susceptibility would be welcome tools in disease eradication efforts, if available. Read More

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Bacteriophage-Based Methods for Detection of Viable subsp. and Their Potential for Diagnosis of Johne's Disease.

Irene R Grant

Front Vet Sci 2021 11;8:632498. Epub 2021 Mar 11.

School of Biological Sciences, Institute for Global Food Security, Queen's University Belfast, Belfast, United Kingdom.

Bacteriophage-based methods for detecting subsp. paratuberculosis (MAP) are a potential new approach for diagnosis of Johne's disease (JD). The basis of these tests is a mycobacteriophage (D29) with a lytic lifecycle that is able to infect a range of spp. Read More

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Alpha-2-Macroglobulin as a New Promising Biomarker Improving the Diagnostic Sensitivity of Bovine Paratuberculosis.

Front Vet Sci 2021 5;8:637716. Epub 2021 Mar 5.

Department of Microbiology, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, South Korea.

Johne's disease (JD) is a chronic granulomatous enteritis of ruminants caused by subsp. (MAP), which induces persistent diarrhea and cachexia. JD causes huge economic losses to the dairy industry due to reduced milk production and premature culling. Read More

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Genomic diversity of Mycobacterium avium subsp. paratuberculosis: pangenomic approach for highlighting unique genomic features with newly constructed complete genomes.

Vet Res 2021 Mar 18;52(1):46. Epub 2021 Mar 18.

School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan, Korea.

Mycobacterium avium subsp. paratuberculosis (MAP) is a causative agent of Johne's disease, which is a chronic granulomatous enteropathy in ruminants. Determining the genetic diversity of MAP is necessary to understand the epidemiology and biology of MAP, as well as establishing disease control strategies. Read More

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Identification and Characterization of and subsp. Zinc Transporters.

J Bacteriol 2021 Mar 15. Epub 2021 Mar 15.

Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Hannover, Germany

Zinc uptake in bacteria is essential to maintain cellular homeostasis and survival. ZnuABC is an important zinc importer of numerous bacterial genera, which is expressed to restore zinc homeostasis when the cytosolic concentration decreases beyond a critical threshold. Upon zinc limitation the fast-growing nonpathogenic organism (MSMEG) as well as the ruminant pathogen subsp. Read More

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