180,278 results match your criteria atpase


Effects of acetylation on dissociation and phosphorylation of actomyosin in postmortem ovine muscle during incubation at 4 °C in vitro.

Food Chem 2021 Mar 27;356:129696. Epub 2021 Mar 27.

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, PR China.

This study aimed to assess the effects of acetylation levels on actomyosin disassociation and phosphorylation of lamb during incubation at 4 °C. Samples of whole proteins from lamb longissimus thoracis muscles were prepared and assigned into three treatments (high, middle and low acetylation groups). The results showed that deacetylation of myosin heavy chain and actin was inhibited by lysine deacetylase inhibitor trichostatin A and nicotinamide in this study. Read More

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Structural basis of drug recognition by the multidrug transporter ABCG2.

J Mol Biol 2021 Apr 7:166980. Epub 2021 Apr 7.

Institute of Molecular Biology and Biophysics, ETH Zurich, Switzerland. Electronic address:

ABCG2 is an ATP-binding cassette (ABC) transporter whose function affects the pharmacokinetics of drugs and contributes to multidrug resistance of cancer cells. While its interaction with the endogenous substrate estrone-3-sulfate (ES) has been elucidated at a structural level, the recognition and recruitment of exogenous compounds is not understood at sufficiently high resolution. Here we present three cryo-EM structures of nanodisc-reconstituted, human ABCG2 bound to anticancer drugs tariquidar, topotecan and mitoxantrone. Read More

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Structure and assembly of archaeal viruses.

Adv Virus Res 2020 5;108:127-164. Epub 2020 Oct 5.

Archaeal Virology Unit, Department of Microbiology, Institut Pasteur, Paris, France. Electronic address:

Viruses of archaea represent one of the most enigmatic parts of the virosphere. Most of the characterized archaeal viruses infect extremophilic hosts and display remarkable diversity of virion morphotypes, many of which have never been observed among bacteriophages or viruses of eukaryotes. However, recent environmental studies have shown that archaeal viruses are widespread also in moderate ecosystems, where they play an important ecological role by influencing the turnover of microbial communities, with a global impact on the carbon and nitrogen cycles. Read More

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October 2020

SOX4 and SMARCA4 cooperatively regulate PI3k signaling through transcriptional activation of TGFBR2.

NPJ Breast Cancer 2021 Apr 9;7(1):40. Epub 2021 Apr 9.

Department of Radiation Oncology, Robert Wood Johnson Medical School, New Brunswick, NJ, USA.

Dysregulation of PI3K/Akt signaling is a dominant feature in basal-like or triple-negative breast cancers (TNBC). However, the mechanisms regulating this pathway are largely unknown in this subset of aggressive tumors. Here we demonstrate that the transcription factor SOX4 is a key regulator of PI3K signaling in TNBC. Read More

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Clutch mechanism of chemomechanical coupling in a DNA resecting motor nuclease.

Proc Natl Acad Sci U S A 2021 Mar;118(11)

Molecular Biology Program, Sloan-Kettering Institute, New York, NY 10065;

Mycobacterial AdnAB is a heterodimeric helicase-nuclease that initiates homologous recombination by resecting DNA double-strand breaks (DSBs). The N-terminal motor domain of the AdnB subunit hydrolyzes ATP to drive rapid and processive 3' to 5' translocation of AdnAB on the tracking DNA strand. ATP hydrolysis is mechanically productive when oscillating protein domain motions synchronized with the ATPase cycle propel the DNA tracking strand forward by a single-nucleotide step, in what is thought to entail a pawl-and-ratchet-like fashion. Read More

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The pentapeptide-repeat protein, MfpA, interacts with mycobacterial DNA gyrase as a DNA T-segment mimic.

Proc Natl Acad Sci U S A 2021 Mar;118(11)

Department of Biological Chemistry, John Innes Centre, NR4 7UH Norwich, United Kingdom;

DNA gyrase, a type II topoisomerase, introduces negative supercoils into DNA using ATP hydrolysis. The highly effective gyrase-targeted drugs, fluoroquinolones (FQs), interrupt gyrase by stabilizing a DNA-cleavage complex, a transient intermediate in the supercoiling cycle, leading to double-stranded DNA breaks. MfpA, a pentapeptide-repeat protein in mycobacteria, protects gyrase from FQs, but its molecular mechanism remains unknown. Read More

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ATP6V0A1 encoding the a1-subunit of the V0 domain of vacuolar H-ATPases is essential for brain development in humans and mice.

Nat Commun 2021 04 8;12(1):2107. Epub 2021 Apr 8.

Department of Biochemistry, Hamamatsu University School of Medicine, Hamamatsu, Japan.

Vacuolar H-ATPases (V-ATPases) transport protons across cellular membranes to acidify various organelles. ATP6V0A1 encodes the a1-subunit of the V0 domain of V-ATPases, which is strongly expressed in neurons. However, its role in brain development is unknown. Read More

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Hsp90 co-chaperones, FKBP52 and Aha1, promote tau pathogenesis in aged wild-type mice.

Acta Neuropathol Commun 2021 Apr 8;9(1):65. Epub 2021 Apr 8.

USF Health Byrd Alzheimer's Institute, University of South Florida, Tampa, FL, 33613, USA.

The microtubule associated protein tau is an intrinsically disordered phosphoprotein that accumulates under pathological conditions leading to formation of neurofibrillary tangles, a hallmark of Alzheimer's disease (AD). The mechanisms that initiate the accumulation of phospho-tau aggregates and filamentous deposits are largely unknown. In the past, our work and others' have shown that molecular chaperones play a crucial role in maintaining protein homeostasis and that imbalance in their levels or activity can drive tau pathogenesis. Read More

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A novel W/O/W double emulsion co-delivering brassinolide and cinnamon essential oil delayed the senescence of broccoli via regulating chlorophyll degradation and energy metabolism.

Food Chem 2021 Mar 27;356:129704. Epub 2021 Mar 27.

Zhejiang University, College of Biosystems Engineering and Food Science, Ningbo Research Institute, Yuhangtang Road 866, Hangzhou 310058, People's Republic of China; Key Laboratory of Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou 310058, People's Republic of China; Fuli Institute of Food Science, Hangzhou 310058, People's Republic of China. Electronic address:

The postharvest senescence accompanied by yellowing limited the shelf-life of broccoli. In this study, we developed a novel W/O/W double emulsion co-delivering brassinolide and cinnamon essential oil and applied it to broccoli for preservation. Results showed that double emulsion prepared by whey protein concentrate-high methoxyl pectin (1:3) exhibited best storage stability with largest particle size (581. Read More

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Chronic electrical stimulation reduces reliance on anaerobic metabolism in locust jumping muscle.

Comp Biochem Physiol A Mol Integr Physiol 2021 Apr 5:110954. Epub 2021 Apr 5.

Department of Biological Sciences, Union College, Schenectady, NY 12308, United States of America.

Chronic electrical stimulation (CES) is a well-documented method for changing mammalian muscle from more fast-twitch to slow-twitch metabolic and contractile profiles. Although both mammalian and insect muscles have many similar anatomical and physiological properties, it is unknown if CES produces similar muscle plasticity changes in insects. To test this idea, we separated Schistocerca americana grasshoppers into two groups (n = 37 to 47): one that was subjected to CES for 180 min each day for five consecutive days and one group that was not. Read More

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ddcP, pstB, and excess D-lactate impact synergism between vancomycin and chlorhexidine against Enterococcus faecium 1,231,410.

PLoS One 2021 8;16(4):e0249631. Epub 2021 Apr 8.

Department of Biological Sciences, University of Texas at Dallas, Richardson, Texas, United States of America.

Vancomycin-resistant enterococci (VRE) are important nosocomial pathogens that cause life-threatening infections. To control hospital-associated infections, skin antisepsis and bathing utilizing chlorhexidine is recommended for VRE patients in acute care hospitals. Previously, we reported that exposure to inhibitory chlorhexidine levels induced the expression of vancomycin resistance genes in VanA-type Enterococcus faecium. Read More

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Atractylenolide I enhances responsiveness to immune checkpoint blockade therapy by activating tumor antigen presentation.

J Clin Invest 2021 Apr 8. Epub 2021 Apr 8.

Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, United States of America.

One of the primary mechanisms of tumor cell immune evasion is the loss of antigenicity, which arises due to lack of immunogenic tumor antigens as well as dysregulation of the antigen processing machinery. In a screen for small-molecule compounds from herbal medicine that potentiate T cell-mediated cytotoxicity, we identified atractylenolide I (ATT-I) that significantly promotes tumor antigen presentation of both human and mouse colorectal cancer (CRC) cells and thereby enhances the cytotoxic response of CD8+ T cells. Cellular thermal shift assay (CETSA) with multiplexed quantitative mass spectrometry identified the proteasome 26S subunit non-ATPase 4 (PSMD4), an essential component of the immunoproteasome complex, as a primary target protein of ATT-I. Read More

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Structural glycoprotein E2 of classical swine fever virus critically interacts with host protein Torsin-1A during the virus infectious cycle.

J Virol 2021 Apr 7. Epub 2021 Apr 7.

Plum Island Animal Disease Center, ARS, USDA, Greenport, NY 11944, USA.

The classical swine fever virus (CSFV) glycoprotein E2 is the major structural component of the virus particle. E2 is involved in several functions such as virus adsorption to the cell, the elicitation of protective immune responses, and virus virulence in swine. Using a yeast two-hybrid system, we previously identified the swine host protein Torsin-1A, an ATPase protein residing in the endoplasmic reticulum and inner nucleus membrane of the cell, as a specific binding partner for E2. Read More

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The structure of Prp2 bound to RNA and ADP-BeF reveals structural features important for RNA unwinding by DEAH-box ATPases.

Acta Crystallogr D Struct Biol 2021 Apr 30;77(Pt 4):496-509. Epub 2021 Mar 30.

Department of Molecular Structural Biology, Institute of Microbiology and Genetics, Göttingen Center for Molecular Biosciences (GZMB), Georg-August-University Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.

Noncoding intron sequences present in precursor mRNAs need to be removed prior to translation, and they are excised via the spliceosome, a multimegadalton molecular machine composed of numerous protein and RNA components. The DEAH-box ATPase Prp2 plays a crucial role during pre-mRNA splicing as it ensures the catalytic activation of the spliceosome. Despite high structural similarity to other spliceosomal DEAH-box helicases, Prp2 does not seem to function as an RNA helicase, but rather as an RNA-dependent ribonucleoprotein particle-modifying ATPase. Read More

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Effects of three kinds of head acupuncture therapies on regulation of brain microenvironment and rehabilitation of nerve function in rats with cerebral palsy.

J Tradit Chin Med 2021 04;41(2):276-283

Department of Child Rehabilitation Division, Huai'an maternal and child care hospital Huai'an, Jiangsu 223002, China.

Objective: To compare and observe the effects of three kinds of cephalic acupuncture therapies commonly used in the clinic on promoting nerve function rehabilitation in the brain microenvironment of rats with cerebral palsy.

Methods: A negative control group, positive control group, and three cephalic acupuncture groups based on the administration of three cephalic acupuncture therapies were established. Ten experimental rats were selected from each group at 1, 2, and 3 weeks after modeling. Read More

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The neonicotinoid alternative sulfoxaflor causes chronic toxicity and impairs mitochondrial energy production in Chironomus kiinensis.

Aquat Toxicol 2021 Mar 28;235:105822. Epub 2021 Mar 28.

School of Environment and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 511443, China.

Unintentional environmental consequences caused by neonicotinoids reinforce the development of safer alternatives. Sulfoxaflor is considered such an alternative. However, ecological risk of sulfoxaflor remains largely unknown. Read More

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Pharmacological activation of SERCA ameliorates dystrophic phenotypes in dystrophin-deficient mdx mice.

Hum Mol Genet 2021 Apr 5. Epub 2021 Apr 5.

Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan.

Duchenne muscular dystrophy (DMD) is an X-linked genetic disorder characterized by progressive muscular weakness due to the loss of dystrophin. Extracellular Ca2+ flows into the cytoplasm through membrane tears in dystrophin-deficient myofibers, which leads to muscle contracture and necrosis. Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) takes up cytosolic Ca2+ into the sarcoplasmic reticulum (SR), but its activity is decreased in dystrophic muscle. Read More

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Dynamic membranes: the multiple roles of P4 and P5 ATPases.

Plant Physiol 2021 Apr;185(3):619-631

Department of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg, Denmark.

The lipid bilayer of biological membranes has a complex composition, including high chemical heterogeneity, the presence of nanodomains of specific lipids, and asymmetry with respect to lipid composition between the two membrane leaflets. In membrane trafficking, membrane vesicles constantly bud off from one membrane compartment and fuse with another, and both budding and fusion events have been proposed to require membrane lipid asymmetry. One mechanism for generating asymmetry in lipid bilayers involves the action of the P4 ATPase family of lipid flippases; these are biological pumps that use ATP as an energy source to flip lipids from one leaflet to the other. Read More

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Zinc Import Mediated by AdcABC is Critical for Colonization of the Dental Biofilm by Streptococcus mutans in an Animal Model.

Mol Oral Microbiol 2021 Apr 5. Epub 2021 Apr 5.

Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, Florida, USA.

Trace metals are essential to all domains of life but toxic when found at high concentrations. While the importance of iron in host-pathogen interactions is firmly established, contemporary studies indicate that other trace metals, including manganese and zinc, are also critical to the infectious process. In this study, we sought to identify and characterize the zinc uptake system(s) of S. Read More

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Changes in gill and air-breathing organ characteristics during the transition from water- to air-breathing in juvenile Arapaima gigas.

J Exp Zool A Ecol Integr Physiol 2021 Apr 5. Epub 2021 Apr 5.

Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada.

The obligate air-breathing Amazonian fish, Arapaima gigas, hatch as water-breathing larvae but with development, they modify their swim bladder to an air-breathing organ (ABO) while reducing their gill filaments to avoid oxygen loss. Here, we show that significant changes already take place between 4 weeks (1.6 g) and 11 weeks (5 g) post hatch, with a reduction in gill lamellar surface area, increase in gill diffusion distance, and proliferation of the parenchyma in the ABO. Read More

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Effects of different temperatures on seawater acclimation in rainbow trout Oncorhynchus mykiss: osmoregulation and branchial phospholipid fatty acid composition.

J Comp Physiol B 2021 Apr 5. Epub 2021 Apr 5.

Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266100, Shandong, China.

This study aimed to investigate the effects of different temperatures on seawater acclimation in rainbow trout (Oncorhynchus mykiss), in terms of growth performance, osmoregulatory capacity, and branchial phospholipid fatty acid (PLFA) composition. The fish (initial weight, 94.73 g) were reared at 9, 12. Read More

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Dead or alive: DEAD-box ATPases as regulators of ribonucleoprotein complex condensation.

Authors:
Karsten Weis

Biol Chem 2021 Apr 2. Epub 2021 Apr 2.

Institute of Biochemistry, ETH Zurich, Otto-Stern-Weg 3, CH-8093Zurich, Switzerland.

DEAD-box ATPase proteins are found in all clades of life and have been associated with a diverse array of RNA-processing reactions in eukaryotes, bacteria and archaea. Their highly conserved core enables them to bind RNA, often in an ATP-dependent manner. In the course of the ATP hydrolysis cycle, they undergo conformational rearrangements, which enable them to unwind short RNA duplexes or remodel RNA-protein complexes. Read More

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The Role of Metal Oxide Nanoparticles, , and on Small Intestinal Enzyme Activity.

Environ Sci Nano 2020 Dec 9;7(12):3940-3964. Epub 2020 Nov 9.

Department of Biomedical Engineering, Binghamton University, Binghamton, NY, 13902, USA.

Engineered nanomaterials (ENMs) have become common in the food industry, which motivates the need to evaluate ENM effects on human health. Gastrointestinal (GI) models (e.g. Read More

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December 2020

Disposition of Proteins and Lipids in Synaptic Membrane Compartments Is Altered in Q175/Q7 Huntington's Disease Mouse Striatum.

Front Synaptic Neurosci 2021 18;13:618391. Epub 2021 Mar 18.

Department of Neurology, Massachusetts General Hospital, Boston, MA, United States.

Dysfunction at synapses is thought to be an early change contributing to cognitive, psychiatric and motor disturbances in Huntington's disease (HD). In neurons, mutant Huntingtin collects in aggregates and distributes to the same sites as wild-type Huntingtin including on membranes and in synapses. In this study, we investigated the biochemical integrity of synapses in HD mouse striatum. Read More

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HBx-upregulated MAFG-AS1 promotes cell proliferation and migration of hepatoma cells by enhancing MAFG expression and stabilizing nonmuscle myosin IIA.

FASEB J 2021 May;35(5):e21529

Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China.

To identify hepatitis B virus (HBV)-related lncRNA(s), we previously examined the transcription profiles of the HBV-transgenic cell line HepG2-4D14 and parental HepG2 cells by RNA deep sequencing and identified 38 upregulated long noncoding RNAs (lncRNAs). In the present study, the lncRNA MAFG-AS1 is investigated in detail because its gene is located adjacent to the MAFG gene, which is an important transcription factor involved in cell proliferation. The level of MAFG-AS1 is significantly higher in HCC tissue than in nontumor tissues. Read More

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A PROSPECTIVE STUDY TO ANALYZE THE SPECIFICITY OF CHLAMYDIAL HEAT SHOCK PROTEIN (CHSP60) ANTIBODIES TO DIAGNOSE TUBAL INFERTILITY.

Wiad Lek 2021 ;74(2):184-189

DEPARTMENT OF OBSTETRICS AND GYNECOLOGY OF POSTGRADUATE DEPARTMENT, BOGOMOLETS NATIONAL MEDICAL UNIVERSITY, KYIV, UKRAINE; DEPARTMENT OF OBSTETRICS AND GYNECOLOGY, KYIV STATE MATERNITY HOSPITAL №5, KYIV, UKRAINEDEPARTMENT OF OBSTETRICS AND GYNECOLOGY OF POSTGRADUATE DEPARTMENT, BOGOMOLETS NATIONAL MEDICAL UNIVERSITY, KYIV, UKRAINE.

Objective: The aim: To investigate the utility of testing for chlamydial heat shock protein 60 (CHSP60) antibodies in the diagnosis of tubal infertility.

Patients And Methods: Materials and methods: All the collected samples were assayed for IgM and IgG antibodies to chlamydia trachomatis and chlamydial heat shock protein 60 (CHSP60) by using immunofluorescence and enzyme-linked immunosorbent assay (ELISA) techniques, respectively.

Results: Results: There were no substantial differences between antibodies to C. Read More

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Suppressing CHD1L reduces the proliferation and chemoresistance in osteosarcoma.

Biochem Biophys Res Commun 2021 Apr 1;554:214-221. Epub 2021 Apr 1.

Department of Orthopedics, The Jinling Hospital of Nanjing, Nanjing, 210002, China. Electronic address:

Osteosarcoma (OS) is the most common bone malignant tumor. However, the genetic basis of OS pathogenesis is still not understood, and occurrence of chemo-resistance is a major reason for the high morbidity of OS patients. Recently, chromodomain helicase/ATPase DNA binding protein 1-like gene (CHD1L) has been identified as a gene related to malignant tumor progression. Read More

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Harpagide exerts a neuroprotective effect by inhibiting endoplasmic reticulum stress via SERCA following oxygen-glucose deprivation/reoxygenation injury.

Neurosci Lett 2021 Apr 1:135874. Epub 2021 Apr 1.

College of Pharmacy, Zhe Jiang Chinese Medical University, Hangzhou, 310053, China. Electronic address:

Cerebrovascular diseases endanger human health, and the physiological and pathological processes of cerebral ischemia/reperfusion injury (CIRI) are critical for the occurrence of these diseases and as targets for their treatment. Here, we evaluated the effects of harpagide-mediated pharmacological and genetic inhibition of sarco-endoplasmic reticulum Ca-ATPase (SERCA) in vitro in PC12 cells. The molecular mechanism by which harpagide protects PC12 cells against oxygen-glucose deprivation/reoxygenation (OGD/R) injury was investigated by evaluating the cell survival rate with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, assessing apoptosis by flow cytometry, determining the intracellular Ca concentration ([Ca]) by laser scanning confocal microscopy (LSCM), and measuring the expression of proteins related to SERCA and endoplasmic reticulum stress (ERS) by Western blotting. Read More

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[Analysis of Clinical Characteristics and Prognosis in Children with Acute Megakaryoblastic Leukemia without Down Syndrome].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Apr;29(2):374-380

Department of Pediatrics, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province,

Objective: To analyze the clinical characteristics and treatment effects of children with acute megakaryoblastic leukemia without down syndrome (non-DS-AMKL).

Methods: The clinical data of 19 children with non-DS-AMKL treated in the Pediatric Hematology Ward in Sun Yat-sen Memorial Hospital of Sun Yat-sen University from May 2008 to April 2018 were analyzed retrospectively. The clinical characteristics, laboratory test and treatment methods of the children were concluded. Read More

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[The Prognostic Factors for AML Children with CBFβ/MYH11 Positive].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Apr;29(2):369-373

Department of Hematology Jinan City People's Hospital, Jinan 271199, Shandong Province, China.

Objective: To analyze the prognostic factors of AML children with CBFβ/MYH11 positive.

Methods: Twenty-eight children with CBFβ/MYH11 positive treated in our hospital from May 2012 to June 2018 were selected, the clinical data and curative were analyzed and evaluated.

Results: Five-year OS and 5-year EFS rate of CBFβ/MYH11 positive AML children was 76. Read More

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