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Dietary Lactobacillus acidophilus ATCC 4356 Relieves the Impacts of Aflatoxin B Toxicity on the Growth Performance, Hepatorenal Functions, and Antioxidative Capacity of Thinlip Grey Mullet (Liza ramada) (Risso 1826).

Probiotics Antimicrob Proteins 2022 Jan 20. Epub 2022 Jan 20.

Animal Production Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh, Egypt.

Dietary Lactobacillus acidophilus ATCC 4356 was used to relieve the impacts of aflatoxin B toxicity on the performances of Liza ramada. The control diet was without any additives, while the second and third diets were supplemented with aflatoxin B at 0.5 and 1 mg/kg. Read More

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January 2022

Randomized control trial on the efficacy of Limosilactobacillus reuteri ATCC PTA 4659 in reducing inflammatory markers in acute uncomplicated diverticulitis.

Eur J Gastroenterol Hepatol 2022 Jan 17. Epub 2022 Jan 17.

Emergency Department - Fondazione Policlinico Universitario A. Gemelli, IRCCS, Roma Emergency Department - Università Cattolica del Sacro Cuore, Roma Ospedale San Carlo di Nancy GVM care and Research, Rome.

Introduction: Recent guidelines suggest treating acute uncomplicated diverticulitis (AUD) without antibiotics. We tested the efficacy of Limosilactobacillus reuteri ATCC PTA 4659 in AUD. Primary outcome was the reduction of abdominal pain and inflammatory markers [C-reactive protein (C-RP) and calprotectin]. Read More

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January 2022

Organic hydroperoxide induces prodigiosin biosynthesis in sp. ATCC 39006 in an OhrR-dependent manner.

Appl Environ Microbiol 2022 Jan 19:AEM0204121. Epub 2022 Jan 19.

Jiangsu Key Laboratory of Phylogenomics & Comparative Genomics, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, Jiangsu Province, China.

The biosynthesis of prodigiosin in the model prodigiosin-producing strain, sp. ATCC 39006, is significantly influenced by environmental and cellular signals. However, a comprehensive regulatory mechanism for this process has not been well established. Read More

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January 2022

Synthesis, Crystal Structure, and Antibacterial Properties of Silver-Functionalized Low-Dimensional Layered Zirconium Phosphonates.

Inorg Chem 2022 Jan 19. Epub 2022 Jan 19.

Department of Pharmaceutical Sciences, University of Perugia, Via del Liceo, 1, 06123 Perugia, Italy.

New insoluble layered zirconium phosphate carboxyaminophosphonates (ZPs), with the general formula Zr(PO)H[(OPCH)N(CH)COO]·HO ( = 3, 4, and 5), have been prepared and characterized. The crystal structure for = 3 and 4 samples was determined from X-ray powder diffraction data. The structure for = 3 was monoclinic in space group 2/ with the following unit cell parameters: = 34. Read More

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January 2022

Developing an understanding of sophorolipid synthesis through application of a central composite design model.

Microb Biotechnol 2022 Jan 17. Epub 2022 Jan 17.

Department of Chemical Engineering and Analytical Science, The University of Manchester, Oxford Road, Manchester, M13 9PL, UK.

A key barrier to market penetration for sophorolipid biosurfactants is the ability to improve productivity and utilize alternative feedstocks to reduce the cost of production. To do this, a suitable screening tool is required that is able to model the interactions between media components and alter conditions to maximize productivity. In the following work, a central composite design is applied to analyse the effects of altering glucose, rapeseed oil, corn steep liquor and ammonium sulphate concentrations on sophorolipid production with Starmerella bombicola ATCC 222144 after 168 h. Read More

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January 2022

Comparative genomics of the black rot pathogen pv. and non-pathogenic co-inhabitant from Trinidad reveal unique pathogenicity determinants and secretion system profiles.

PeerJ 2022 3;9:e12632. Epub 2022 Jan 3.

Department of Life Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.

Black-rot disease caused by the phytopathogen pv. (Xcc) continues to have considerable impacts on the productivity of cruciferous crops in Trinidad and Tobago and the wider Caribbean region. While the widespread occurrence of resistance of Xcc against bactericidal agrochemicals can contribute to the high disease burdens, the role of virulence and pathogenicity features of local strains on disease prevalence and severity has not been investigated yet. Read More

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January 2022

A Combined In Vitro and In Vivo Assessment of the Safety of the Yeast Strains Kluyveromyces marxianus A4 and A5 Isolated from Korean Kefir.

Probiotics Antimicrob Proteins 2022 Jan 16. Epub 2022 Jan 16.

Center for One Health, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, South Korea.

Kefir is a traditional fermented milk containing beneficial bacteria and yeasts. Despite Kluyveromyces marxianus, isolated from kefir, gaining increasing attention as a potential probiotic yeast owing to its biological function, Saccharomyces boulardii is the only species considered as a probiotic yeast. We evaluated the safety of K. Read More

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January 2022

A study of various factors affecting satellitism tests of Haemophilus influenzae and Haemophilus parainfluenzae using Staphylococcus aureus as the source of NAD.

J Microbiol Methods 2022 Jan 13;193:106417. Epub 2022 Jan 13.

R&D Center, Autobio Diagnostics Co., Ltd, Zhengzhou, Henan, China. Electronic address:

Many factors affecting satellitism tests are unclear, and it is difficult to avoid misidentification, even if the medium is properly selected. We investigated the factors causing false-positive results for Haemophilus influenzae and false-negative results for Haemophilus parainfluenzae in the satellitism tests using Staphylococcus aureus as the source of nicotinamide adenine dinucleotide (NAD). H. Read More

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January 2022

Evaluation of the substitution of poliomyelitis virus for testing virucidal activities of instrument and surface disinfection.

J Hosp Infect 2022 Jan 13. Epub 2022 Jan 13.

Deutsche Vereinigung zur Bekämpfung der Viruskrankheiten, Berlin, Germany.

The Global Polio Eradication initiative has the goal to eradicate poliomyelitis worldwide. This means that poliomyelitisvirus type 1 strain LSc 2ab (PV-1) can no longer be used for the evaluation of virucidal activity of chemical disinfectants. In this study, we evaluated murine parvovirus ATCC VR 1346 (minute virus of mice, MVM) as suitable surrogate for PV-1 when testing virucidal activity of biocides in instrument and surface disinfectants. Read More

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January 2022

Attenuation of Enterococcus faecalis biofilm formation by Rhodethrin: A combinatorial study with an antibiotic.

Microb Pathog 2022 Jan 13;163:105401. Epub 2022 Jan 13.

Department of Genomic Science, School of Biological Sciences, Central University of Kerala, Tejaswini Hills, Periya (P.O), Kasaragod, Kerala, 671320, India. Electronic address:

The nosocomial pathogen Enterococcus faecalis critically implicated in the hospital environment. Its major virulence attributes biofilm formation and antibiotic resistance. The novel therapeutics are required to inhibit E. Read More

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January 2022

Expression, Purification, and Characterization of the Recombinant, Two-Component, Response Regulator ArlR from Fusobacterium nucleatum.

Appl Biochem Biotechnol 2022 Jan 14. Epub 2022 Jan 14.

Key Laboratory of Biotechnology and Bioresources Utilization (Ministry of Education), College of Life Science, Dalian Minzu University, Dalian, China.

Fusobacterium nucleatum is associated with the incidence and development of multiple diseases, such as periodontitis and colorectal cancer (CRC). Until now, studies have proved only a few proteins to be associated with such pathogenic diseases. The two-component system is one of the most prevalent forms of bacterial signal transduction related to intestinal diseases. Read More

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January 2022

De-novo fabrication of sunlight irradiated silver nanoparticles and their efficacy against E. coli and S. epidermidis.

Sci Rep 2022 Jan 13;12(1):676. Epub 2022 Jan 13.

Department of Electrical and Computer Engineering, School of Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.

Silver nanoparticles (AgNPs) gained significant attention due to their activity against microbial pathogens, cancer cells, and viral particles etc. Traditional fabrication methods require hazardous chemicals as reducing agents and their usage and disposal pose a significant hazard to environmental ecosystem. Here, a de novo, robust, cost effective and an eco-friendly method is reported to fabricate AgNPs irradiated with sunlight (SL) while using Salvadora persica root extract (SPE) as reducing agent. Read More

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January 2022

The fish pathogen Flavobacterium columnare represents four distinct species: Flavobacterium columnare, Flavobacterium covae sp. nov., Flavobacterium davisii sp. nov. and Flavobacterium oreochromis sp. nov., and emended description of Flavobacterium columnare.

Syst Appl Microbiol 2021 Dec 23;45(2):126293. Epub 2021 Dec 23.

Washington Animal Disease Diagnostic Laboratory, Pullman, WA, United States; Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, United States.

Flavobacterium columnare is the causative agent of columnaris disease in freshwater fish and four discrete genetic groups exist within the species, suggesting that the species designation requires revision. The present study determined the taxonomic status of the four genetic groups of F. columnare using polyphasic and phylogenomic approaches and included five representative isolates from each genetic group (including type strain ATCC 23463; genetic group 1). Read More

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December 2021

A short plus long-amplicon based sequencing approach improves genomic coverage and variant detection in the SARS-CoV-2 genome.

PLoS One 2022 13;17(1):e0261014. Epub 2022 Jan 13.

Department of Immunology, University of Texas Southwestern Medical Center, Dallas, TX, United States of America.

High viral transmission in the COVID-19 pandemic has enabled SARS-CoV-2 to acquire new mutations that may impact genome sequencing methods. The ARTIC.v3 primer pool that amplifies short amplicons in a multiplex-PCR reaction is one of the most widely used methods for sequencing the SARS-CoV-2 genome. Read More

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January 2022

A practical approach to producing isomaltomegalosaccharide using dextran dextrinase from Gluconobacter oxydans ATCC 11894.

Appl Microbiol Biotechnol 2022 Jan 13;106(2):689-698. Epub 2022 Jan 13.

Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan.

Dextran dextrinase (DDase) catalyzes formation of the polysaccharide dextran from maltodextrin. During the synthesis of dextran, DDase also generates the beneficial material isomaltomegalosaccharide (IMS). The term megalosaccharide is used for a saccharide having DP = 10-100 or 10-200 (DP, degree of polymerization). Read More

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January 2022

Essential Oils of Six Achillea Species: Chemical Profiles, Antimicrobial Potential and Toxicity toward Crustaceans.

Chem Biodivers 2022 Jan 13. Epub 2022 Jan 13.

University of Niš: Univerzitet u Nisu, Department of Biology and Ecology, Višegradska 33, 18000, Niš, SERBIA.

This is the first comparative study on antimicrobial activity of essential oils (EOs) of six Achillea species ( A. crithmifolia , A. distans , A. Read More

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January 2022

Heterogeneous vancomycin resistance in Staphylococcus aureus does not predict development of vancomycin resistance upon vancomycin pressure.

J Antimicrob Chemother 2022 Jan 11. Epub 2022 Jan 11.

Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, Lyon, France.

Background: It is unclear whether Staphylococcus aureus with heterogeneous intermediate vancomycin resistance (hVISA) can develop vancomycin resistance faster than vancomycin-susceptible S. aureus (VSSA) strains.

Methods: We compared the kinetics of vancomycin MIC increase for 15 days of sustained in vitro vancomycin exposure for clinical hVISA (n = 12) and VSSA (n = 24) isolates, as well as for reference strains Mu3 (hVISA) and ATCC 29213 (VSSA). Read More

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January 2022

In vitro evaluation of EDTA combined with photodynamic therapy to reduce Streptococcus mutans in carious dentin.

Photodiagnosis Photodyn Ther 2022 Jan 10;37:102718. Epub 2022 Jan 10.

Center for Health Sciences, PUC- Campinas, Campinas, SP, Brazil. Electronic address:

Background: This in vitro study aimed to evaluate the use of EDTA combined with photodynamic therapy to reduce Streptococcus mutans in carious dentin.

Methods: Sixty third molars were sectioned to obtain flat dentin surfaces. All specimens were waterproofed, except for the coronal dentin, and subjected to cariogenic challenge in brain-heart infusion (BHI) broth supplemented with 0. Read More

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January 2022

MntP and YiiP Contribute to Manganese Efflux in Salmonella enterica Serovar Typhimurium under Conditions of Manganese Overload and Nitrosative Stress.

Microbiol Spectr 2022 Jan 12:e0131621. Epub 2022 Jan 12.

Rhodes Collegegrid.262541.6 Biology Department, Memphis, Tennessee, USA.

The divalent transition metal cation manganese is important for protein function, particularly under conditions of iron limitation, nitrosative stress, and oxidative stress, but can mediate substantial toxicity in excess. Salmonella enterica serovar Typhimurium possesses multiple manganese importers, but the pathways for manganese efflux remain poorly defined. The . Read More

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January 2022

Induction of innate immune responses by KPC-producing of the pandemic sequence type 258-clade I.

Access Microbiol 2021 2;3(11):000275. Epub 2021 Nov 2.

Infectious Diseases Research Laboratory, 4th Department of Internal Medicine, School of Medicine, National and Kapodistrian University of Athens, General University Hospital "Attikon", Athens, Greece.

-carbapenemase-producing (KPC) sequence-type 258 (ST258) has emerged as an important human pathogen throughout the world. Although lacking known virulence factors, it is associated with significant morbidity and high mortality rates. The pathogenicity of KPC ST258 strains has not been fully elucidated yet. Read More

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November 2021

Antimicrobial Efficacy of Green Synthesis of Silver Nanoparticles against Cariogenic Pathogens - An Study.

J Pharm Bioallied Sci 2021 Nov 10;13(Suppl 2):S1188-S1192. Epub 2021 Nov 10.

Department of Conservative, Dentistry and Endodontics, Vivekanandha Dental College for Women, Namakkal, The Tamil Nadu Dr. M.G.R. Medical University, Tamil Nadu, India.

Background: Nowadays, emergence of drug resistance might happen in the world. Hence, invention of new dental material had started by researchers for the same. The present study evaluated the antimicrobial property of green synthesized nanosilver particles against dental cariogenic microorganisms such as and . Read More

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November 2021

Emerione A, a novel fungal metabolite as an inhibitor of New Delhi metallo-β-lactamase-1, restores carbapenem susceptibility in carbapenem-resistant isolates.

J Glob Antimicrob Resist 2022 Jan 8. Epub 2022 Jan 8.

Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China; Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China. Electronic address:

Objectives: Bacterial strains that produce New Delhi metal-β-lactamase 1 (NDM-1) are worldwide threats. It is still a challenging task to find a potent NDM-1 inhibitor for clinical practice.

Methods: Molecular docking and virtual screening of an in-house fungal natural product database for NDM-1 inhibitors were performed. Read More

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January 2022

Isolation and Characterization of Chitosans from Different Fungi with Special Emphasis on Zygomycetous Dimorphic Fungus : Evaluation of Its Chitosan Nanoparticles for the Inhibition of Human Pathogenic Fungi.

Biomacromolecules 2022 Jan 11. Epub 2022 Jan 11.

Greenvention Biotech Pvt. Ltd., Pune 412202, India.

The cell wall chitosan was extracted from fungi belonging to different taxonomic classes, namely, (Zygomycetes, dimorphic), , , , and (Ascomycetes, yeasts), and and (Basidiomycetes). The maximum yield of chitosan was 60.89 ± 2. Read More

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January 2022

Proteomic analysis of hexahydro-β-acids/hydroxypropyl-β-cyclodextrin inhibit Listeria monocytogenes.

Appl Microbiol Biotechnol 2022 Jan 11;106(2):755-771. Epub 2022 Jan 11.

School of Chemical Engineering and Technology, Xinjiang University, Urumqi, 830046, China.

Food safety affected by food-borne pathogen has received increasing attention by researchers. Listeria monocytogenes (L. monocytogenes), widespread in the environment, could easily cause some diseases. Read More

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January 2022

Hackflex: low-cost, high-throughput, Illumina Nextera Flex library construction.

Microb Genom 2022 Jan;8(1)

iThree Institute, University of Technology Sydney, Sydney, NSW, Australia.

We developed a low-cost method for the production of Illumina-compatible sequencing libraries that allows up to 14 times more libraries for high-throughput Illumina sequencing to be generated for the same cost. We call this new method Hackflex. The quality of library preparation was tested by constructing libraries from MG1655 genomic DNA using either Hackflex, standard Nextera Flex (recently renamed as Illumina DNA Prep) or a variation of standard Nextera Flex in which the bead-linked transposase is diluted prior to use. Read More

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January 2022

Characterization of cisplatin-loaded chitosan nanoparticles and rituximab-linked surfaces as target-specific injectable nano-formulations for combating cancer.

Sci Rep 2022 Jan 10;12(1):468. Epub 2022 Jan 10.

Department of Pharmaceutics, College of Pharmacy, Jazan University, Jazan, Saudi Arabia.

The present study was carried out to develop cisplatin-loaded chitosan nanoparticles (CCNP) and cisplatin-loaded chitosan nanoparticle surface linked to rituximab (mAbCCNP) as targeted delivery formulations. The two formulations (CCNP and mAbCCNP) exhibited significant physicochemical properties. The zetapotential (ZP) values of CCNP and mAbCCNP were 30. Read More

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January 2022

Biosynthesis and Characterization of Extracellular Silver Nanoparticles from : Antimicrobial, Anti Larval, and Anticancer Activities.

Molecules 2021 Dec 30;27(1). Epub 2021 Dec 30.

Agricultural Microbiology Department, Faculty of Agriculture, Ain Shams University, Cairo 11566, Egypt.

The ability of microorganisms to reduce inorganic metals has launched an exciting eco-friendly approach towards developing green nanotechnology. Thus, the synthesis of metal nanoparticles through a biological approach is an important aspect of current nanotechnology. In this study, ATCC 14921 gave the small particle of silver nanoparticles (AgNPs) a size of 38. Read More

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December 2021

Isolate MK212927: A Natural Producer of Allylamine Antifungal 'Terbinafine'.

Molecules 2021 Dec 29;27(1). Epub 2021 Dec 29.

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity St., Cairo 11566, Egypt.

Resistance to antifungal agents represents a major clinical challenge, leading to high morbidity and mortality rates, especially in immunocompromised patients. In this study, we screened soil bacterial isolates for the capability of producing metabolites with antifungal activities via the cross-streak and agar cup-plate methods. One isolate, coded S6, showed observable antifungal activity against ATCC 10231 and clinical isolate. Read More

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December 2021

() Hairy Roots with Antigenotoxic and Anti-Photogenotoxic Activity.

Molecules 2021 Dec 29;27(1). Epub 2021 Dec 29.

Department of Pharmaceutical Biology and Medicinal Plant Biotechnology, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha St., 02-097 Warsaw, Poland.

Hairy root cultures are considered as a valuable source of bioactive phytoconstituents with expanding applicability for their production. In the present study, hairy root cultures of (), a traditional Southeast Asian medicinal plant, were established. The transformation with ATCC 15834 allowed to obtain 15 root lines. Read More

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December 2021

Ionic Silver and Electrical Treatment for Susceptibility and Disinfection of Biofilm-Contaminated Titanium Surface.

Molecules 2021 Dec 28;27(1). Epub 2021 Dec 28.

Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand.

In this work, surface disinfection and biofilm susceptibility were investigated by applying ionic silver of 0.4-1.6 µg/mL and cathodic voltage-controlled electrical treatment of 1. Read More

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December 2021