128,678 results match your criteria assays vitro

In vitro cellular activity of maghemite/cerium oxide magnetic nanoparticles with antioxidant properties.

Colloids Surf B Biointerfaces 2021 May 6;204:111824. Epub 2021 May 6.

Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 06, Prague 6, Czech Republic. Electronic address:

Magnetic γ-FeO/CeO nanoparticles were obtained by precipitation of Ce(NO) with ammonia in the presence of γ-FeO seeds. The formation of CeO nanoparticles on the seeds was confirmed by transmission electron microscopy linked with selected area electron diffraction, energy-dispersive X-ray spectroscopy, electron energy loss spectroscopy, and dynamic light scattering. The γ-FeO/CeO particle surface was functionalized with PEG-neridronate to improve the colloidal stability in PBS and biocompatibility. Read More

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The lncRNA RP3-439F8.1 promotes GBM cell proliferation and progression by sponging miR-139-5p to upregulate NR5A2.

Pathol Res Pract 2021 Jan 2;223:153319. Epub 2021 Jan 2.

Department of Neurosurgery, Yunnan Cancer Hospital, Kunming, China. Electronic address:

Background: Nuclear Receptor Subfamily 5 Group A Member 2 (NR5A2, LRH-1) is an oncogene in a wide range of cancer types. Bioinformatics analysis on glioblastoma multiforme (GBM) tumors has revealed that the miR-139-5p-NR5A2 axis may be putatively regulated by the long non-coding RNA (lncRNA) RP3-439F8.1. Read More

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January 2021

Brazilian Red Propolis shows antifungal and immunomodulatory activities against Paracoccidioides brasiliensis.

J Ethnopharmacol 2021 May 12:114181. Epub 2021 May 12.

Federal University of Alfenas - UNIFAL. Alfenas, MG, Brazil. Electronic address:

Ethnopharmacological Relevance: Paracoccidioidomycosis (PCM) is a systemic mycosis with high prevalence in South America and especially in Brazil with severe clinical consequences that need broadened therapeutic options. Propolis is a natural resin from bees used in folk medicine for centuries with the first report in the ancient history of Egypt by Eberly papyrus, in Middle-Ages used to wash the newborn's umbilical cord and World War II as antiseptic or antibiotics. Nowadays it is a natural product worldwide consumed as food and traditionally used for oral and systemic diseases as an anti-inflammatory, antimicrobial, antifungal, and other diseases. Read More

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The anti-inflammatory effects of an ethanolic extract of the rhizome of Atractylodes lancea, involves Akt/NF-κB signaling pathway inhibition.

J Ethnopharmacol 2021 May 12:114183. Epub 2021 May 12.

Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China; Research and Development Center for Natural Health Products, Shenzhen Institute of Research and Continuing Education, China. Electronic address:

Ethnopharmacological Relevance: The dried rhizome of Atractylodes lancea (Thumb.) DC. (Compositae) has been prescribed in folk medicine for the management of various inflammatory conditions such as rheumatic diseases, gastritis and hepatitis. Read More

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Postprandial administration but not controlled release in the colon increases oral bioavailability of DF030263, a promising drug candidate for chronic lymphocytic leukemia.

Eur J Pharm Biopharm 2021 May 12. Epub 2021 May 12.

School of Pharmacy & Centre for Biomolecular Sciences, University of Nottingham, Nottingham, UK. Electronic address:

For treatment of chronic cancers, the oral administration route is preferred as it provides numerous advantages over other delivery routes. However, these benefits of oral chemotherapy can be limited due to unfavorable pharmacokinetics. Accordingly, pharmacokinetic development of chemotherapeutic agents is crucial to the improvement of cancer treatment. Read More

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Mouse totipotent stem cells captured and maintained through spliceosomal repression.

Cell 2021 May 10. Epub 2021 May 10.

MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking University, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China. Electronic address:

Since establishment of the first embryonic stem cells (ESCs), in vitro culture of totipotent cells functionally and molecularly comparable with in vivo blastomeres with embryonic and extraembryonic developmental potential has been a challenge. Here we report that spliceosomal repression in mouse ESCs drives a pluripotent-to-totipotent state transition. Using the splicing inhibitor pladienolide B, we achieve stable in vitro culture of totipotent ESCs comparable at molecular levels with 2- and 4-cell blastomeres, which we call totipotent blastomere-like cells (TBLCs). Read More

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The role of the HIF-1α/ALYREF/PKM2 axis in glycolysis and tumorigenesis of bladder cancer.

Cancer Commun (Lond) 2021 May 15. Epub 2021 May 15.

Department of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210000, P. R. China.

Background: As a rate-limiting enzyme of glycolysis, pyruvate kinase muscle isozyme M2 (PKM2) participates in tumor metabolism and growth. The regulatory network of PKM2 in cancer is complex and has not been fully studied in bladder cancer. The 5-methylcytidine (m5C) modification in PKM2 mRNA might participate in the pathogenesis of bladder cancer and need to be further clarified. Read More

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Photobiomodulation of oral fibroblasts stimulated with periodontal pathogens.

Lasers Med Sci 2021 May 15. Epub 2021 May 15.

School of Dentistry, University of Birmingham, Birmingham, UK.

Photobiomodulation (PBM) utilises light energy to treat oral disease, periodontitis. However, there remains inconsistency in the reporting of treatment parameters and a lack of knowledge as to how PBM elicits its molecular effects in vitro. Therefore, this study aimed to establish the potential immunomodulatory effects of blue and near infra-red light irradiation on gingival fibroblasts (GFs), a key cell involved in the pathogenesis of periodontitis. Read More

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Circ_CHFR promotes PDGF-BB-induced proliferation, invasion and migration in VSMCs via miR-149-5p/NRP2 axis.

J Cardiovasc Pharmacol 2021 May 10. Epub 2021 May 10.

Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi'an Medical University, Xi'an, 710077, Shaanxi, China Department of General Practice, The First Affiliated Hospital of Xi'an Medical University, Xi'an, 710077, Shaanxi, China.

Abstract: Circular RNA Checkpoint With Forkhead And Ring Finger Domains (circ_CHFR) was reported to regulate vascular smooth muscle cell (VSMC) dysfunction during atherosclerosis (AS). However, the molecule mechanism of circ_CHFR in AS remains largely unclear. Human VSMCs (HVSMCs) were exposed to platelet-derived growth factor-bb (PDGF-BB) in vitro. Read More

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Antifungal Azoles as Tetracycline Resistance-Modifiers in .

Appl Environ Microbiol 2021 May 14. Epub 2021 May 14.

Clinical Microbiology & Antimicrobial Research Laboratory, CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh-160036, India

has developed resistance to antimicrobials since its first use. The major facilitator superfamily (MFS) efflux pump Tet(K) contributes to resistance to tetracyclines. The efflux pump diminishes antibiotic accumulation, and biofilm hampers the diffusion of antibiotics. Read More

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Evaluation of anticancer effects in vitro of new iridium(III) complexes targeting the mitochondria.

J Inorg Biochem 2021 May 11;221:111465. Epub 2021 May 11.

School of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, PR China. Electronic address:

Iridium(III) complexes have the potential to serve as novel therapeutic drugs for treating tumor. In this work, three new complexes [Ir(ppy)(cdppz)](PF) (1) (ppy = 2-phenylpyridine, cdppz = 11-chlorodipyrido[3,2-a,2',3'-c]phenazine), [Ir(bzq)(cdppz)](PF) (2) (bzq = benzo[h]quinolone) and [Ir(piq)(cdppz)](PF) (3) (piq = 1-phenylisoquinoline) were prepared as well as characterized. MTT (3-(4,5-dimethylthiazole)-2,5-diphenyltetraazolium bromide) assay revealed that the complex 2 exerted potent cytotoxicity against to various cancer cells lines and particularly for SGC-7901 cells. Read More

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Systematic review of Ethiopian medicinal plants used for their anti-inflammatory and wound healing activities.

J Ethnopharmacol 2021 May 11:114179. Epub 2021 May 11.

Centre for Global Health Research, Brighton and Sussex Medical School, University of Sussex, Brighton, BN1 9PX, United Kingdom; School of Public Health, Addis Ababa University, Addis Ababa, Ethiopia.

Ethnopharmacological Relevance: Plant materials are used worldwide as complementary and alternative therapeutics for the treatment of various illnesses. In Ethiopia, folk medicines are utilized across a wide range of cultures and settings. Ethiopia has numerous plant species of which around 12% are endemic, making it a rich source of medicinal plants that are potentially important for human wellbeing. Read More

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SOX9 promotes epidermal growth factor receptor-tyrosine kinase inhibitor resistance via targeting β-catenin and epithelial to mesenchymal transition in lung cancer.

Life Sci 2021 May 11:119608. Epub 2021 May 11.

Cancer Drug Resistance Laboratory, Department of Life Science, National Institute of Technology Rourkela, Odisha 769008, India. Electronic address:

Aims: The first-generation epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI), gefitinib, continues to be a primary treatment option for lung cancer patients. However, acquisition of resistance to gefitinib is a major obstacle in lung cancer treatment and its cause is poorly understood. The present study aimed to implicate the role of SOX9-β-catenin in developed resistance to gefitinib through epithelial to mesenchymal transition (EMT) in lung cancer in vitro and ex vitro. Read More

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Superoxide Dismutase in Extender Improves the in vitro Quality and in vivo Fertility of Cryopreserved Water Buffalo (Bubalus bubalis) Spermatozoa.

Cryo Letters 2020 Jul-Aug;41(4):194-201

Animal Reproduction Laboratory, Animal Sciences Institute, National Agricultural Research Center, Islamabad, Pakistan.

Background: Superoxide dismutase (SOD) as an antioxidant in semen extender may be used for the cryopreservation of buffalo spermatozoa and in vivo fertility.

Objective: This study was aimed to evaluate the effects of SOD (SOD1, 100 IU/mL; SOD2, 200 IU/mL; SOD3, 300 IU/mL) and control (0.0) in Tris citric acid extender on in vitro quality and in vivo fertility of cryopreserved water buffalo bull spermatozoa. Read More

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Sulfasalazine, a potent suppressor of gastric cancer proliferation and metastasis by inhibition of xCT: Conventional drug in new use.

J Cell Mol Med 2021 May 14. Epub 2021 May 14.

Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.

The aim of this study was to explore the role of sulfasalazine on proliferation and metastasis in gastric cancer by inhibition of xCT. The relationships between clinical characteristics and xCT expression were analysed. An immunohistochemical staining assay and Western blot were performed among gastric cancers and normal gastric tissues. Read More

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Lysophosphatidylcholine 17:1 from the Leaf Surface of the Wild Potato Species Inhibits .

J Agric Food Chem 2021 May 14. Epub 2021 May 14.

Department Biochemistry of Plant Interactions, Leibniz Institute of Plant Biochemistry, Weinberg 3, Halle (Saale) D-06120, Germany.

Late blight, caused by the oomycete , is economically the most important foliar disease of potato. To assess the importance of the leaf surface, as the site of the first encounter of pathogen and host, we performed untargeted profiling by liquid chromatography-mass spectrometry of leaf surface metabolites of the susceptible cultivated potato and the resistant wild potato species . Hydroxycinnamic acid amides, typical phytoalexins of potato, were abundant on the surface of , but not on . Read More

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The circular RNA circ_GRHPR promotes NSCLC cell proliferation and invasion via interactions with the RNA-binding protein PCBP2.

Clin Exp Pharmacol Physiol 2021 May 13. Epub 2021 May 13.

Department of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.

As the most common malignancy, lung cancer is characterised by high rates of occurrence and mortality. Although circular RNAs (circRNAs) are known to act as important regulators in cancer, their role in lung cancer remains poorly understood. In this study, circ_GRHPR expression was found to be significantly upregulated in the serum of five patients with non-small cell lung cancer (NSCLC), compared to that in healthy controls. Read More

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hsa-miR-199a-3p Inhibits Motility, Invasiveness, and Contractility of Ovarian Endometriotic Stromal Cells.

Reprod Sci 2021 May 13. Epub 2021 May 13.

Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, Idaigaoka 1-1, Hasama-machi, Yufu-shi, Oita, 879-5593, Japan.

It is suggested that aberrantly expressed microRNAs are involved in the pathogenesis of endometriosis. Our previous study demonstrated that expression of the microRNA hsa-miR-199a-3p is attenuated in human endometriotic cyst stromal cells (ECSCs). The current study aimed to define the roles of hsa-miR-199a-3p in the development of endometriosis. Read More

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Adiponectin receptor agonist AdipoRon ameliorates renal inflammation in diet-induced obese mice and endotoxin-treated human glomeruli ex vivo.

Diabetologia 2021 May 14. Epub 2021 May 14.

Research Program for Clinical and Molecular Metabolism, Faculty of Medicine, University of Helsinki, Helsinki, Finland.

Aims/hypothesis: Chronic low-grade inflammation with local upregulation of proinflammatory molecules plays a role in the progression of obesity-related renal injury. Reduced serum concentration of anti-inflammatory adiponectin may promote chronic inflammation. Here, we investigated the potential anti-inflammatory and renoprotective effects and mechanisms of action of AdipoRon, an adiponectin receptor agonist. Read More

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-Amylase and -Glucosidase Inhibitory and Antioxidant Activities of the Crude Extract and Solvent Fractions of Leaves.

Biomed Res Int 2021 19;2021:6652777. Epub 2021 Apr 19.

Department of Pharmacy, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia.

Background: The leaves of have been used in the management of diabetes mellitus in Ethiopian folk medicine. Thus, this study is aimed at investigating the -amylase and -glucosidase inhibitory and antioxidant activities of the crude extract and solvent fractions of leaves.

Methods: The -amylase and -glucosidase inhibitory and antioxidant activities of the plant extract were assessed using 3,5-dinitrosalicylic acid (DNSA), p-nitro-phenyl-a-D glucopyranoside (p-NPG), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays, respectively. Read More

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The G199X and V157fs mutations in the gene promote malignancy in serous ovarian cancer: an analysis using whole-exome sequencing.

Ann Transl Med 2021 Apr;9(8):710

Department of Gynecology and Obstetrics, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, China.

Background: The carcinogenic mechanisms underlying serous ovarian cancer are not fully understood.

Methods: Whole-exome sequencing and targeted sequencing were performed in ovarian cancer samples to identify novel molecular markers involved in the process of cell malignancy in ovarian cancer. experiments, the oncogenic roles such as cell proliferation, migration and invasion of G199X and V157fs mutations were investigated deeply. Read More

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PSMD12 promotes glioma progression by upregulating the expression of Nrf2.

Ann Transl Med 2021 Apr;9(8):700

Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, China.

Background: Glioma is the most common and aggressive primary brain tumor in adults. Proteasome 26S subunit, non-ATPase 12 (PSMD12), an important subunit in the 26S proteasome, is known to be involved in the growth and apoptosis of breast cancer cells. However, its exact function and underlying molecular mechanisms in glioma remain unknown. Read More

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Inhibition of bone morphogenetic protein receptor 2 suppresses pancreatic ductal adenocarcinoma growth by regulating GRB2/PI3K/AKT axis.

Ann Transl Med 2021 Apr;9(7):557

Department of General Surgery, Peking University First Hospital, Beijing, China.

Background: Bone morphogenetic protein receptor 2 (BMPR2) is an important transmembrane serine/threonine kinase that involves oncogenic processes in multiple cancers. However, the role of BMPR2 and its regulatory mechanism in pancreatic ductal adenocarcinoma (PDAC) remain unknown.

Methods: We performed a tissue array to explore the expression of BMPR2 in PDAC tissues. Read More

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Centromere protein N may be a novel malignant prognostic biomarker for hepatocellular carcinoma.

PeerJ 2021 3;9:e11342. Epub 2021 May 3.

Hubei Cancer Clinical Study Center, Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan, China.

Background: Hepatocellular carcinoma (HCC) is one of the deadliest tumors. The majority of HCC is detected in the late stage, and the clinical results for HCC patients are poor. There is an urgent need to discover early diagnostic biomarkers and potential therapeutic targets for HCC. Read More

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Antioxidant Activities of (Mart.) J. B. Gillett) (Burseraceae) Leaf Extracts Using and Assays.

Oxid Med Cell Longev 2021 24;2021:3043720. Epub 2021 Apr 24.

Laboratório de Transformação de Plantas e Análise em Microscopia (LTPAM), Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59078-970, Brazil.

is widely used in folk medicine without any scientific basis. Considering this, the aim of this study was to evaluate the chemical profile and the antioxidant activity of leaf extracts using and assays. Six extracts were obtained from fresh leaves using a serial extraction (nonpolar to polar solvents). Read More

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Long non-coding RNA USP30-AS1 aggravates the malignant progression of cervical cancer by sequestering microRNA-299-3p and thereby overexpressing PTP4A1.

Oncol Lett 2021 Jul 29;22(1):505. Epub 2021 Apr 29.

Department of Gynaecology, The First People's Hospital of Chongqing Liangjiang New Area, Chongqing 401120, P.R. China.

USP30 antisense RNA 1 (USP30-AS1) has been studied in bladder urothelial carcinoma. However, the detailed role of USP30-AS1 in cervical cancer remains to be elucidated. Therefore, the present study determined whether USP30-AS1 is implicated in cervical cancer malignancy, and investigated relevant molecular mechanisms. Read More

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Overexpression of miRNA-22-3p attenuates osteoporosis by targeting MAPK14.

Exp Ther Med 2021 Jul 2;22(1):692. Epub 2021 May 2.

Department of Orthopaedics, Chongqing Public Health Medical Center, Chongqing 400036, P.R. China.

Osteoporosis (OP) results from an imbalance between bone formation, which is regulated by osteoblasts, and bone resorption, which is mediated by osteoclasts. MicroRNA-22-3p (miR-22-3p) expression is decreased during the process of osteoclast differentiation and p38α mitogen-activated protein kinase (MAPK)14 promotes the proliferation and differentiation of osteoclast progenitors. However, whether miR-22-3p could target MAPK14 to regulate the progression of OP remains unknown, which was the aim of the present study. Read More

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NK Cells and PMN-MDSCs in the Graft From G-CSF Mobilized Haploidentical Donors Display Distinct Gene Expression Profiles From Those of the Non-Mobilized Counterpart.

Front Immunol 2021 27;12:657329. Epub 2021 Apr 27.

Immunology Research Area, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.

A recent approach of hematopoietic stem cell (HSC) transplantation from haploidentical donors "mobilized" with G-CSF is based on the selective depletion of T and B lymphocytes from the graft. Through this approach, the patient receives both HSC and mature donor-derived effector cells (including NK cells), which exert both anti-leukemia activity and protection against infections. We previously showed that donor HSC mobilization with G-CSF results in accumulation in the graft of polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs), capable of inhibiting the anti-leukemia activity of allogeneic NK cells. Read More

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Interactions of spp. Root Extracts and Alkylamides With the Endocannabinoid System and Peripheral Inflammatory Pain.

Front Pharmacol 2021 27;12:651292. Epub 2021 Apr 27.

Department of Biology, University of Ottawa, Ottawa, ON, Canada.

Historical ethnobotanies of indigenous peoples of the North American prairies reveal treatment of many painful conditions by spp. Recent evidence suggests a pharmacological basis for such use as the bioactivity of and is mediated, in part, through activation of the endocannabinoid system (ECS). Whereas the cannabimimetic effects of individual echinacea products and alkylamides have been described, the activity of crude extracts has not been compared between cannabinoid (CB) receptors or across species or genotypes. Read More

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Prediction of Synergistic Drug Combinations for Prostate Cancer by Transcriptomic and Network Characteristics.

Front Pharmacol 2021 12;12:634097. Epub 2021 Apr 12.

College of Chemistry, Sichuan University, Chengdu, China.

Prostate cancer (PRAD) is a major cause of cancer-related deaths. Current monotherapies show limited efficacy due to often rapidly emerging resistance. Combination therapies could provide an alternative solution to address this problem with enhanced therapeutic effect, reduced cytotoxicity, and delayed the appearance of drug resistance. Read More

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