50,354 results match your criteria assayed determination

Association between body temperature and leukocyte telomere length in middle-aged and older adults.

Epidemiol Health 2021 Sep 8:e2021063. Epub 2021 Sep 8.

Department of Foods and Nutrition, College of Natural Sciences, Kookmin University, 02707 Seoul, Republic of Korea.

Objectives: Data on the association between body temperature (BT) and leukocyte telomere length (LTL), which has been widely used as a biomarker of cellular senescence in recent epidemiological studies, are limited. Aims: The present study aimed to explore the association between a normal BT range (35.0 to 37. Read More

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September 2021

Assessment of Red Cell Distribution Width, Platelet/lymphocyte Ratio, Systemic Immune-inflammation Index, and Neutrophil/lymphocyte Ratio Values in Patients with Central Retinal Artery Occlusion.

Ocul Immunol Inflamm 2021 Sep 15:1-5. Epub 2021 Sep 15.

Department of Ophthalmology, Sakarya Training and Research Hospital, Sakarya, Turkey.

Purpose: To evaluate complete blood count (CBC)-derived inflammatory indices in central retinal artery occlusion (CRAO).

Methods: A total of 42 patients with CRAO (CRAO group) and 42 age- and sex-matched subjects without CRAO (Control group) were included. CBC parameters including red cell distribution width (RDW) levels were assayed. Read More

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September 2021

A qualitative and quantitative comparison of IgE antibody profiles with two multiplex platforms for component resolved diagnostics in allergic patients.

Clin Exp Allergy 2021 Sep 14. Epub 2021 Sep 14.

Clinical and Laboratory Molecular Allergy Unit - IDI - IRCCS, Rome, Italy.

Background: Clinically complex phenotypes require more and more sophisticated and comprehensive diagnostic approaches, able to discriminate genuine sensitizations from cross-reactivity. Interpretative complexity of multiplex diagnostic arrays has somewhat limited their diffusion. This study compares two currently available methods, namely ISAC® test and ALEX2® test. Read More

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September 2021

EPA and DHA confer protection against DON-induced endoplasmic reticulum stress and iron imbalance in IPEC-1 cells.

Br J Nutr 2021 Sep 14:1-29. Epub 2021 Sep 14.

Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, China 430023.

This study assessed the molecular mechanism of eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) protection against IPEC-1 cell damage induced by deoxynivalenol (DON). The cells were divided into six groups, including the CON group, the EPA group, the DHA group, the DON group, the EPA+DON group, and the DHA+DON group. RNA sequencing was used to investigate the potential mechanism, and qRT-PCR was employed to verify the expression of selected genes. Read More

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September 2021

Protein tyrosine phosphatase, receptor type B is a potential biomarker and facilitates cervical cancer metastasis via epithelial-mesenchymal transition.

Bioengineered 2021 Dec;12(1):5739-5748

Department of Radiotherapy, The Huizhou Municipal Central Hospital, Huizhou, Guangdong, China.

Cervical cancer (CC) is one of the most common malignant tumors. This study analyzed the impact of protein tyrosine phosphatase, receptor type B (PTPRB) on malignant behavior of CC and explored its possible molecular mechanism. RT-PCR, western blot and Immunohistochemistry were applied to examine the expression of PTPRB in CC specimens and cells. Read More

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December 2021

Exploration of the Potential Mechanism of Tao Hong Si Wu Decoction for the Treatment of Breast Cancer Based on Network Pharmacology and Experimental Verification.

Front Oncol 2021 26;11:731522. Epub 2021 Aug 26.

The First Affiliated Hospital of Anhui University of Chinese Medicinee, Hefei, China.

Background: Tao Hong Si Wu Decoction (THSWD) is a well-known traditional Chinese medicine used clinically alone or combined with drugs to treat breast cancer. However, there has been no study to date on the underlying mechanisms of its therapeutic effects.

Objectives: To explore the potential mechanism of THSWD for the treatment of breast cancer using network pharmacology and experimental research. Read More

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Hybrids of 4-hydroxy derivatives of goniothalamin and piplartine bearing a diester or a 1,2,3-triazole linker as antiproliferative agents.

Bioorg Chem 2021 Aug 25;116:105292. Epub 2021 Aug 25.

Department of Organic Chemistry, Institute of Chemistry, University of Campinas, UNICAMP, 13083-970 Campinas, Sao Paulo, Brazil. Electronic address:

A library of nine hybrids of 4-hydroxygoniothalamin (2), 4-hydroxypiplartine (4), monastrol (5) and oxo-monastrol (6) was prepared via a modular synthetic route with a diester or a 1,2,3-triazole as linkers. The compounds were assayed against a panel of human cancer cell lines, including MCF-7 (breast adenocarcinoma), HeLa (cervical adenocarcinoma), Caco-2 (colorectal adenocarcinoma) and PC3 (prostate adenocarcinoma), as well as against normal breast (MCF10A) and prostate (PNT2) cells. In general, hybrids with an ester linker containing 4-hydroxypiplartine (4) were more potent than the corresponding hybrids with 4-hydroxygoniothalamin (2). Read More

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FT-IR Study, Thermal Analysis, and Evaluation of the Antibacterial Activity of a MK-Geopolymer Mortar Using Glass Waste as Fine Aggregate.

Polymers (Basel) 2021 Aug 31;13(17). Epub 2021 Aug 31.

Department of Engineering, University of Campania "Luigi Vanvitelli", Via Roma n. 29, 81031 Aversa, Italy.

Food containers made from glass are separately collected from urban solid waste at 76% in most parts of Europe. The cullet glass finds its way to re-melting, while the debris is often disposed of. With this contribution, we suggest an upcycling process where glass debris is simply ground without any washing operation and added to an alkali-activated paste. Read More

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A Robust and Highly Precise Alternative against the Proliferation of Intestinal Carcinoma and Human Hepatocellular Carcinoma Cells Based on Lanthanum Strontium Manganite Nanoparticles.

Materials (Basel) 2021 Aug 31;14(17). Epub 2021 Aug 31.

Central Metallurgical Research and Development Institute, P.O. Box 87, Helwan 11912, Cairo, Egypt.

In this report, lanthanum strontium manganite at different Sr ion concentrations, as well as Gd or Sm ion substituted LaMSrMnO (M = Gd and Sm, = 0.2), have been purposefully tailored using a sol gel auto-combustion approach. XRD profiles confirmed the formation of a monoclinic perovskite phase. Read More

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The Chemical Profiling of Essential Oils from Different Tissues of L. and Their Antimicrobial Activities.

Molecules 2021 Aug 24;26(17). Epub 2021 Aug 24.

Aromatic Plant Research Center, 230 N 1200 E, Suite 100, Lehi, UT 84043, USA.

L. is grown as an ornamental plant, used as raw material for furniture, as a source of camphor, and its essential oil can be used as an important source for perfume as well as alternative medicine. A comparative investigation of essential oil compositions and antimicrobial activities of different tissues of was carried out. Read More

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Auxiliary Subunits Regulate the Dendritic Turnover of AMPA Receptors in Mouse Hippocampal Neurons.

Front Mol Neurosci 2021 23;14:728498. Epub 2021 Aug 23.

Institute for Physiology, Otto-von-Guericke University, Magdeburg, Germany.

Different families of auxiliary subunits regulate the function and trafficking of native α (AMPA) receptors in the central nervous system. While a facilitatory role of auxiliary subunits in ER export and forward trafficking of newly synthesized AMPA receptors is firmly established, it is unclear whether auxiliary subunits also control endosomal receptor turnover in dendrites. Here, we manipulated the composition of AMPA receptor complexes in cultured hippocampal neurons by overexpression of two auxiliary subunits, (TARP) γ-8 or (CKAMP) 44a, and monitored dendritic receptor cycling in live-cell imaging experiments. Read More

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Expression regulation of chitin-binding protein and metal-binding peptide in new Bacillus velezensis: MALDI-TOF MS/MS analysis.

J Basic Microbiol 2021 Sep 8. Epub 2021 Sep 8.

Department of Cellular and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.

Metallothionein and metal-binding peptides are small cysteine-rich proteins produced by different organisms in stress conditions. In this study, the metal-binding peptide was detected in extracellular proteins of a new Bacillus velezensis strain, isolated from metal contaminated soil, and grown on the lead-enriched medium, for the first time. The presence of sulfide peptide was assayed by two simple tests (lead sulfide and Ellman's reagent test) for preliminary, and subsequently confirmed using polyacrylamide gel electrophoresis at media with different lead concentrations that the low-molecular-weight protein fragments (≈10 kDa) were observed while none were detected in the medium containing sodium chloride or calcium salt. Read More

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September 2021

Trace analysis and identification of 33 sulfonamides and sulfonamide potentiators in eggs by ultrahigh-performance liquid chromatography coupled with quadrupole-high-field orbitrap high-resolution mass spectrometry.

Anal Methods 2021 Sep 8. Epub 2021 Sep 8.

Institute of Quality Standards and Testing Technology for Agricultural Product, Chinese Academy of Agricultural Science, Beijing, 100081, China.

A method was established for the simultaneous trace analysis and identification of 27 sulfonamides and 6 sulfonamide potentiators in eggs by ultrahigh-performance liquid chromatography coupled with quadrupole-high-field orbitrap high-resolution mass spectrometry and multifunction impurity adsorption cleaning. The appropriate extraction, purification, and instrument conditions were optimized. The drugs were extracted by acetonitrile with 1% formic acid and purified with optimized adsorption material based on multifunction impurity adsorption cleaning. Read More

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September 2021

Mithramycin delivery systems to develop effective therapies in sarcomas.

J Nanobiotechnology 2021 Sep 6;19(1):267. Epub 2021 Sep 6.

Sarcomas and Experimental Therapeutics Laboratory, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Hospital Universitario Central de Asturias, Avenida de Roma, s/n, 33011, Oviedo, Spain.

Background: Sarcomas comprise a group of aggressive malignancies with very little treatment options beyond standard chemotherapy. Reposition of approved drugs represents an attractive approach to identify effective therapeutic compounds. One example is mithramycin (MTM), a natural antibiotic which has demonstrated a strong antitumour activity in several tumour types, including sarcomas. Read More

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September 2021

Potential of inInhibiting Nitric Oxide Synthase.

Pak J Biol Sci 2021 Jan;24(8):840-846

<b>Background and Objective:</b> Inflammation occurs <i>via</i> several mechanisms, one of which includes the production of Nitric Oxide (NO) catalyzed by inducible nitric oxide synthase (iNOS), which is inhibited selectively by isothioureas. <i>Ageratum conyzoides</i> L. has shown activity in reducing pain and inflammation, although the molecular mechanism had not been undertaken. Read More

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January 2021

The additive value of copeptin for early diagnosis and prognosis of acute coronary syndromes.

Am J Emerg Med 2021 Aug 30;50:413-421. Epub 2021 Aug 30.

Cardiology Department, Assiut University Heart Hospital, Egypt.

Background: One promising biomarker that has received substantial interest for the evaluation of suspected acute coronary syndromes (ACS) is copeptin. Therefore, our goal was to assess the additive value of copeptin for early diagnosis and prognosis of Non-ST segment acute coronary syndromes (NSTE-ACS).

Methods: The study included ninety patients with suspected ACS. Read More

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Hypoxia-induced DEC1 mediates trophoblast cell proliferation and migration via HIF1α signaling pathway.

Tissue Cell 2021 Aug 12;73:101616. Epub 2021 Aug 12.

Department of Clinical Laboratory Medicine, Shandong Provincial Qianfoshan Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China. Electronic address:

In early pregnancy, hypoxia is a typical extrinsic factor that regulates EVT functions including proliferation, migration and invasion which are essential for a successful pregnancy. Human differentiated embryonic chondrocyte-expressed gene 1 (DEC1), a hypoxia-regulated gene, has been reported to be overexpressed in several types of cancers. Given that the placenta and the cancer share several similarities with respect to their capacity to proliferate and invade adjacent tissues, we focused on the role of DEC1 on trophoblast function in a physiologically hypoxic environment, which may be associated with unexplained recurrent spontaneous abortion (URSA). Read More

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Reduced sensitivity to thyroid hormone is associated with diabetes and hypertension.

J Clin Endocrinol Metab 2021 Sep 4. Epub 2021 Sep 4.

Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, I. R. Iran.

Background: Recently, reduced sensitivity to thyroid hormone as a more common finding in the general population and its possible association with metabolic parameters has been the focus of attention. We evaluated the cross-sectional association of thyroid hormone sensitivity with diabetes, metabolic syndrome (MetS), and its components.

Methods: The study included a Tehranian representative sample of 5124 subjects aged ≥20 years participating in the Tehran Thyroid Study (2008-2011). Read More

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September 2021

Dynamic changes of activated AHR in microglia and astrocytes in the substantia nigra-striatum system in an MPTP-induced Parkinson's disease mouse model.

Brain Res Bull 2021 Sep 1;176:174-183. Epub 2021 Sep 1.

Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, China; Department of Neurodegeneration and Injury, Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, China. Electronic address:

Aryl Hydrocarbon Receptor (AHR) is a ligand-activated transcription factor expressed in various brain regions. However, little is known about the role of AHR during neuroinflammation in the 1-methyl-4-phenyl-1,2,3,6-tetrathydropyridine (MPTP)-induced Parkinson's disease (PD) mouse model. Here, mice were sacrificed at day 4, day 6 and day 8 respectively after MPTP or saline treatment. Read More

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September 2021

Accessing Molecular Dimeric Ir Water Oxidation Catalysts from Coordination Precursors.

Inorg Chem 2021 Sep 3. Epub 2021 Sep 3.

Department of Chemistry, Yale University, 225 Prospect Street, New Haven, Connecticut 06520, United States.

One ongoing challenge in the field of iridium-based water oxidation catalysts is to develop a molecular precatalyst affording well-defined homogeneous active species for catalysis. Our previous work by using organometallic precatalysts Cp*Ir(pyalk)OH and Ir(pyalk)(CO) (pyalk = (2-pyridyl)-2-propanolate) suggested a μ-oxo-bridged Ir dimer as the probable resting state, although the structure of the active species remained elusive. During the activation, the ligands Cp* and CO were found to oxidatively degrade into acetic acid or other products, which coordinate to Ir centers and affect the catalytic reaction. Read More

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September 2021

Microbial aerosol particles in four seasons of sanitary landfill site: Molecular approaches, traceability and risk assessment.

J Environ Sci (China) 2021 Oct 4;108:120-133. Epub 2021 Mar 4.

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Landfill sites are regarded as prominent sources of bioaerosols for the surrounding atmosphere. The present study focused on the emission of airborne bacteria and fungi in four seasons of a sanitary landfill site. The main species found in bioaerosols were assayed using high-throughput sequencing. Read More

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October 2021

Associations Between Occupational Status, Support at Work, and Salivary Cortisol Levels.

Int J Behav Med 2021 Aug 30. Epub 2021 Aug 30.

Public Health, Department of Social Medicine, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita-shi, Osaka, 565-0871, Japan.

Objective: The present study investigated associations between occupation, job stress, and salivary cortisol levels after psychological tasks.

Methods: We examined 766 (273 men and 493 women) healthy employed Japanese participants aged 21 to 68 years (mean age = 46.4 years, standard deviation = 8. Read More

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A Promising, Novel Radiosensitizer Nanodrug Complex for Oral Cavity Cancer: Cetuximab and Cisplatin-Conjugated Gold Nanoparticles.

Balkan Med J 2021 Aug 31. Epub 2021 Aug 31.

Department of Translational Oncology, Institute of Oncology, Dokuz Eylül University, İzmir, Turkey.

Background: Nanomedicine has provided promising tools for the imaging, diagnosis, and treatment of cancer. Gold nanoparticles (GNPs) may be useful in enhancing the efficacy of radiotherapy, such as radiosensitization, in cancer therapy.

Aims: To develop a nanodrug complex containing cetuximab (C225,CTX) and cisplatin (CDDP) conjugated with GNPs and to investigate its cytotoxic effects on oral cavity cancer cells when combined with radiotherapy. Read More

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Chikungunya in Guerrero, Mexico, 2019 and Evidence of Gross Underreporting in the Region.

Am J Trop Med Hyg 2021 Aug 30. Epub 2021 Aug 30.

Veterinary Microbiology & Preventive Medicine, Iowa State University, Ames, Iowa.

The local public health authorities reported nine cases of chikungunya in Mexico in 2019, none of which occurred in Guerrero, a coastal state in the southwest. To test the hypothesis that chikungunya is grossly underreported in Mexico, acute sera were collected from 639 febrile patients from low-income households in Guerrero in 2019 and serologically assayed for chikungunya virus (CHIKV). Analysis of the sera by plaque reduction neutralization test revealed that 181 (28. Read More

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Preventive effect of a Kampo medicine, kososan, on recurrent depression in a mouse model of repeated social defeat stress.

Gene 2022 Jan 26;806:145920. Epub 2021 Aug 26.

Oriental Medicine Research Center, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan.

Depression is deemed a mood disorder characterized by a high rate of relapse. Therefore, overcoming of the recurrent depression is globally expecting. Kososan, a traditional Japanese herbal medicine, has been clinically used for mild depressive mood, and our previous studies have shown some evidence for its antidepressive-like efficacy in experimental animal models of depression. Read More

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January 2022

Comparison of the Efficacy of Disinfectant Pre-impregnated Wipes for Decontaminating Stainless Steel Carriers Experimentally Inoculated With Ebola Virus and Vesicular Stomatitis Virus.

Front Public Health 2021 10;9:657443. Epub 2021 Aug 10.

Reckitt Benckiser LLC, Global Research & Development for Lysol and Dettol, Montvale, NJ, United States.

The authors evaluated four disinfectant pre-impregnated wipes (DPW) for efficacy against Ebola virus Makona variant (EBOV) and vesicular stomatitis virus (VSV), Indiana serotype. Steel carriers were inoculated with the infectious virus and then were wiped with DPW in the Wiperator instrument per ASTM E2967-15. Following the use of J-Cloth impregnated with medium (negative control wipes) or the use of activated hydrogen peroxide (AHP)-, ethanol-, sodium hypochlorite (NaOCl)-, or single or dual quaternary ammonium compound (QAC)-based DPW, virus recovery from the carriers was assayed by titration assay and by two passages on Vero E6 cells in 6-well plates. Read More

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Screening Anti-Inflammatory Effects of Flavanones Solutions.

Int J Mol Sci 2021 Aug 18;22(16). Epub 2021 Aug 18.

Center for Chemical Research, Institute for Research Basic and Applied Sciences, Autonomous University of the State of Morelos, Av. Universidad 1001, Cuernavaca 62209, Mexico.

There are a large number of remedies in traditional medicine focused on relieving pain and inflammation. Flavanones have been a potential source in the search for leading compounds and biologically active components, and they have been the focus of much research and development in recent years. is used in traditional medicine for the treatment of kidney diseases, bladder infections, and diabetes mellitus. Read More

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Modulation of the PGE-Mediated Pathway in the Eclosion Blocking Effect of Flumethrin and Terpenoid Subfraction Isolated from in .

Molecules 2021 Aug 13;26(16). Epub 2021 Aug 13.

Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Lakkidi, P. O., Wayanad 673576, Kerala, India.

Prostaglandins are a group of important cell-signaling molecules involved in the regulation of ovarian maturation, oocyte development, egg laying and associated behaviors in invertebrates. However, the presence of prostaglandin E (PGE), the key enzymes for PGE biosynthesis and its interference by drugs were not investigated previously in the ovary of ticks. The present study was undertaken to assess the modulation of the PGE-mediated pathway in the eclosion blocking effect of flumethrin and terpenoid subfraction isolated from in ticks. Read More

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Determination of Oxidized Lipids in Commonly Consumed Foods and a Preliminary Analysis of Their Binding Affinity to PPARγ.

Foods 2021 Jul 22;10(8). Epub 2021 Jul 22.

Department of Nutrition, Byrdine F. Lewis College of Nursing and Health Professions, Georgia State University, Atlanta, GA 30302, USA.

Foods rich in poly unsaturated fatty acids (PUFA) are vulnerable to oxidation. While it is well established that endogenously derived oxidized lipids are ligands of the transcription factor PPARγ, the binding ability of diet-derived oxidized lipids is unknown. Our two-fold objective was to determine the oxidized lipid content and PPARγ binding ability of commonly consumed foods. Read More

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Impact of Plasma Xanthine Oxidoreductase Activity on the Mechanisms of Distal Symmetric Polyneuropathy Development in Patients with Type 2 Diabetes.

Biomedicines 2021 Aug 19;9(8). Epub 2021 Aug 19.

Department of Pharmacotherapy, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose 204-8588, Tokyo, Japan.

To unravel associations between plasma xanthine oxidoreductase (XOR) and diabetic vascular complications, especially distal symmetric polyneuropathy (DSP), we investigated plasma XOR activities using a novel assay. Patients with type 2 diabetes mellitus (T2DM) with available nerve conduction study (NCS) data were analyzed. None were currently taking XOR inhibitors. Read More

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