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Isolation, characterization, and antimicrobial activity of communic acid from .

J Complement Integr Med 2021 Jul 29. Epub 2021 Jul 29.

Laboratory of Organic Synthesis, Extraction and Valorisation, Faculty of Sciences Aïn Chock, Hassan II University of Casablanca, BP. 20000, Casablanca, Morocco.

Objectives: A mixture of Z and E communic acid is isolated for the first time from the cones of Its biological activity was studied.

Methods: The plant material was extracted in a Soxhlet apparatus with -hexane, the resulting extract was subjected to column chromatography (CC) on silica gel. The structure elucidation of the constituents of the isolated fraction was identified by comparison of its spectroscopic properties H and C NMR data with those reported in the literature. Read More

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DeepCervix: A deep learning-based framework for the classification of cervical cells using hybrid deep feature fusion techniques.

Comput Biol Med 2021 Jul 20;136:104649. Epub 2021 Jul 20.

Cancer Hospital of China Medical University, Liaoning Hospital and Institute, Shenyang, 110042, China.

Cervical cancer, one of the most common fatal cancers among women, can be prevented by regular screening to detect any precancerous lesions at early stages and treat them. Pap smear test is a widely performed screening technique for early detection of cervical cancer, whereas this manual screening method suffers from high false-positive results because of human errors. To improve the manual screening practice, machine learning (ML) and deep learning (DL) based computer-aided diagnostic (CAD) systems have been investigated widely to classify cervical Pap cells. Read More

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Should temozolomide be used on the basis of O-methylguanine DNA methyltransferase status in patients with advanced neuroendocrine tumors? A systematic review and meta-analysis.

Cancer Treat Rev 2021 Jul 22;99:102261. Epub 2021 Jul 22.

IEO, European Institute of Oncology, IRCCS, Milan, Italy. Electronic address:

Background: Temozolomide (TEM) is an active treatment in metastatic neuroendocrine tumors (NETs). Patients affected by glioblastoma multiforme or advanced melanoma treated with TEM who have deficiency of O-methylguanine DNA methyltransferase (MGMT) have a better responses and survival. However, the predictive role of MGMT in patients with NETs treated with TEM is still debated. Read More

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Missed HIV diagnoses when screening only emergency department patients who have blood samples obtained for other clinical purposes.

Am J Emerg Med 2021 Jul 21;50:102-105. Epub 2021 Jul 21.

Department of Emergency Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA. Electronic address:

Objective: Emergency departments (EDs) are highly valued settings for HIV screening. Most large-volume ED HIV screening programs have attenuated operational barriers by screening only ED patients who already have a blood sample available for other clinical reasons. Our objective was to estimate the proportion of HIV positive patients who are missed when an ED excludes patients for whom HIV screening would be the only indication to obtain a blood sample. Read More

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Endotyping asthma related to three different work exposures.

J Allergy Clin Immunol 2021 Jul 28. Epub 2021 Jul 28.

Faculty of Medicine, Human Microbiome Research Program, University of Helsinki, Helsinki, Finland; Institute of Environmental Medicine (IMM), Karolinska Institutet, Stockholm, Sweden.

Background: Work exposures play a significant role in adult-onset asthma, but mechanisms of work-related asthma are not fully elucidated.

Objective: We aimed to reveal the molecular mechanisms of work-related asthma associated with flour (FA), isocyanate (IA) or welding fume (WA) exposures and identify potential biomarkers that distinguish these groups from each other.

Methods: We used a combination of clinical tests, transcriptomic analysis and associated pathway analyses to investigate underlying disease mechanisms of the blood immune cells and the airway epithelium of 61 men. Read More

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Maternal immune activation leads to atypical turning asymmetry and reduced DRD2 mRNA expression in a rat model of schizophrenia.

Behav Brain Res 2021 Jul 28:113504. Epub 2021 Jul 28.

Division of Experimental and Molecular Psychiatry, Department of Psychiatry, Psychotherapy and Preventive Medicine, LWL University Hospital, Ruhr-University Bochum, Germany. Electronic address:

Atypical asymmetries have been reported in individuals diagnosed with schizophrenia, linking higher symptom severity to weaker lateralization. Furthermore, both lateralization and schizophrenia are influenced by the dopaminergic system. However, whether a direct link between the etiology of schizophrenia and atypical asymmetries exists is yet to be investigated. Read More

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Short-term exposure to nitrogen dioxide and mortality: A systematic review and meta-analysis.

Environ Res 2021 Jul 28:111766. Epub 2021 Jul 28.

Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, USA. Electronic address:

Background: Ambient air pollution has been characterized as a leading cause of mortality worldwide and has been associated with cardiovascular and respiratory diseases. There is increasing evidence that short-term exposure to nitrogen dioxide (NO), is related to adverse health effects and mortality.

Methods: We conducted a systematic review of short-term NO and daily mortality, which were indexed in PubMed and Embase up to June 2021. Read More

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Development and Validation of a Simplified Risk Score for the Prediction of Critical COVID-19 Illness in Newly Diagnosed Patients.

J Med Virol 2021 Jul 31. Epub 2021 Jul 31.

Department of Nephrology, Technical University of Munich, School of Medicine, Klinikum rechts der Isar, Munich, Germany.

Objectives: Scores to identify patients at high risk of progression of coronavirus disease (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), may become instrumental for clinical decision-making and patient management.

Methods: We used patient data from the multicentre Lean European Open Survey on SARS-CoV-2-Infected Patients (LEOSS) and applied variable selection to develop a simplified scoring system to identify patients at increased risk of critical illness or death. A total of 1,946 patients who tested positive for SARS-CoV-2 were included in the initial analysis and assigned to derivation and validation cohorts (n=1,297 and n=649, respectively). Read More

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Two-way comparison of brain perfusion image processing software for patients with acute ischemic strokes in real-world.

Neuroradiology 2021 Jul 31. Epub 2021 Jul 31.

Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang, 110004, People's Republic of China.

Purpose: Perfusion imaging generates multimaps of ischemic tissues and is a proven decision-making tool in patients with acute ischemic stroke. However, the reliability of perfusion post-processing outcomes has been debated, given disparate results of various software applications, especially for patients with small ischemic core volume. This study was undertaken to compare ischemic volume estimates determined by imSTROKE (a software with new imaging protocol) and RAPID computer applications, respectively. Read More

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Defining the Role of Cellular Immune Signatures in Diagnostic Evaluation of Suspected Tuberculosis.

J Infect Dis 2021 Jul 31. Epub 2021 Jul 31.

TB Research Centre, National Heart and Lung Institute, Imperial College London, London, United Kingdom.

Background: Diagnosis of paucibacillary tuberculosis (TB) including extrapulmonary TB is a significant challenge, particularly in high-income, low-incidence settings. Measurement of Mycobacterium tuberculosis (Mtb)-specific cellular immune signatures by flow cytometry discriminates active TB from latent TB infection (LTBI) in case-control studies; however, their diagnostic accuracy and clinical utility in routine clinical practice is unknown.

Methods: Using a nested case-control study design within a prospective multicenter cohort of patients presenting with suspected TB in England, we assessed diagnostic accuracy of signatures in 134 patients who tested interferon-gamma release assay (IGRA)-positive and had final diagnoses of TB or non-TB diseases with coincident LTBI. Read More

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High-Throughput Feeding Bioassay for Lepidoptera Larvae.

J Chem Ecol 2021 Jul 31. Epub 2021 Jul 31.

Université Paris-Saclay, CNRS, IRD, UMR Évolution, Génomes, Comportement et Écologie, 91198, Gif-sur-Yvette, France.

Finding plant cultivars that are resistant or tolerant against lepidopteran pests, takes time, effort and is costly. We present here a high throughput leaf-disk consumption assay system, to screen plants for resistance or chemicals for their deterrence. A webcam capturing images at regular intervals can follow the feeding activities of 150 larvae placed into individual cages. Read More

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Hepatozoon in Eurasian red squirrels Sciurus vulgaris, its taxonomic identity, and phylogenetic placement.

Parasitol Res 2021 Jul 31. Epub 2021 Jul 31.

CEITEC, University of Veterinary Sciences, Brno, Czech Republic.

Adeleorid apicomplexan parasites of the genus Hepatozoon Miller, 1908 are broadly distributed among the rodents. Broader molecular data on Hepatozoon from Palaearctic squirrels are necessary for evaluation of diversity and origin of Hepatozoon in Eurasian red squirrel Sciurus vulgaris populations, considering ongoing invasion by Gray squirrel S. carolinensis. Read More

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Validation of nasospheroids to assay CFTR functionality and modulator responses in cystic fibrosis.

Sci Rep 2021 Jul 30;11(1):15511. Epub 2021 Jul 30.

Medicine Genetics Group, Vall D'Hebron Research Institute, 08035, Barcelona, Spain.

The availability of a simple, robust and non-invasive in vitro airway model would be useful to study the functionality of the cystic fibrosis transmembrane regulator (CFTR) protein and to personalize modulator therapy for cystic fibrosis (CF) patients. Our aim was to validate a CFTR functional study using nasospheroids, a patient-derived nasal cell 3D-culture. We performed live-cell experiments in nasospheroids obtained from wild-type individuals and CF patients with different genotypes and phenotypes. Read More

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Characterisation of seizure induction methods in .

eNeuro 2021 Jul 30. Epub 2021 Jul 30.

Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Manchester, M13 9PL, UK

Epilepsy is one of the most common neurological disorders. Around one third of patients do not respond to current medications. This lack of treatment indicates a need for better understanding of the underlying mechanisms and, importantly, the identification of novel targets for drug manipulation. Read More

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Global patterns of enhancer activity during sea urchin embryogenesis assessed by eRNA profiling.

Genome Res 2021 Jul 30. Epub 2021 Jul 30.

Carnegie Mellon University

We used CAGE-seq (Capped Analysis of Gene Expression with Sequencing) to profile eRNA expression and enhancer activity during embryogenesis of a model echinoderm- the sea urchin, We identified >18,000 enhancers that were active in mature oocytes and developing embryos and documented a burst of enhancer activation during cleavage and early blastula stages. We found that a large fraction (73.8%) of all enhancers active during the first 48 hr of embryogenesis were hyperaccessible no later than the 128-cell stage, and possibly even earlier. Read More

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Neural evidence for representational persistence within events.

J Neurosci 2021 Jul 30. Epub 2021 Jul 30.

Department of Psychology, Columbia University, New York, NY 10027.

How does the brain process continuous experiences so they can be remembered? Evidence suggests that people perceive their experience as a series of distinct and meaningful events. Information encountered within the same event shows greater temporal integration in memory, as well as enhanced neural representational similarity. Although these data support the theory that the brain builds and maintains a mental model of the current event that represents recently encountered stimulus information, this hypothesis has not been directly tested. Read More

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Preoperative and perioperative intervention reduces the risk of recurrence of endometriosis in mice caused by either incomplete excision or spillage and dissemination.

Reprod Biomed Online 2021 Apr 29. Epub 2021 Apr 29.

Shanghai OB/GYN Hospital, Fudan University, Shanghai 200011, China; Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Fudan University, Shanghai, China. Electronic address:

Research Question: Can preoperative or perioperative intervention reduce the risk of recurrence of endometriosis caused by either incomplete excision or spillage and dissemination?

Design: A mouse model of endometriosis recurrence caused by spillage and dissemination was first established using 24 female Balb/c mice. The spillage and dissemination model was used to test the efficacy of preoperative use of ketorolac, perioperative use of aprepitant and combined use of propranolol and andrographolide in a prospective, randomized mouse experiment involving 75 mice. The efficacy of these preoperative and perioperative interventions in a mouse recurrence model caused by incomplete excision was also tested using 72 mice. Read More

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Coronary Artery Bypass Grafting in Patients With High Risk of Bleeding.

Heart Lung Circ 2021 Jul 27. Epub 2021 Jul 27.

Research Unit of Surgery, Anesthesiology and Critical Care, University of Oulu, Oulu, Finland; Heart and Lung Center, Helsinki University Hospital, Helsinki, Finland; Department of Surgery, University of Turku, Turku, Finland.

Background: Postoperative bleeding after cardiac surgery is associated with increased morbidity and mortality. We tested the hypothesis that patients with a preoperatively estimated high risk of severe perioperative bleeding may have impaired early outcome after on-pump versus off-pump coronary artery bypass grafting (CABG).

Method: Data from 7,352 consecutive patients who underwent isolated CABG from January 2015 to May 2017 were included in the multicentre European Coronary Artery Bypass Grafting registry. Read More

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Molecular features and vulnerabilities of recurrent chordomas.

J Exp Clin Cancer Res 2021 Jul 30;40(1):244. Epub 2021 Jul 30.

Institute of Pathology, University Hospital Ulm, Albert-Einstein-Allee 11, 89081, Ulm, Germany.

Background: Tumor recurrence is one of the major challenges in clinical management of chordoma. Despite R0-resection, approximately 50% of chordomas recur within ten years after initial surgery. The underlying molecular processes are poorly understood resulting in the lack of associated therapeutic options. Read More

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Assessing the antigenicity of different VP3 regions of infectious bursal disease virus in chickens from South Brazil.

BMC Vet Res 2021 Jul 30;17(1):259. Epub 2021 Jul 30.

Instituto Carlos Chagas/ICC, Fundação Oswaldo Cruz/Fiocruz, Curitiba, PR, Brazil.

Background: Infectious bursal disease (IBD), also known as Gumboro disease, is a viral infection that causes mortality and immunosuppression in chickens (Gallus gallus). VP2 and VP3 are the major structural viral capsid components and are the most immunogenic proteins of IBD virus (IBDV). Reliable diagnostic tests using VP2 and VP3 produced in heterologous systems are important tools to control this infection. Read More

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First Report of Cucurbit chlorotic yellows virus infecting Cucumber Plants in Spain.

Plant Dis 2021 Jul 30. Epub 2021 Jul 30.

ValGenetics, Plant Pathology and Microbiology Laboratory, Paterna, Valencia, Spain.

During the winter 2018, symptoms of leaf chlorotic spots (Figure 1) followed by symptoms of leaf interveinal chlorosis (Figure 2) and severe chlorosis in basal leaves were observed in cucumber cv Laredo (Cucumis sativus) plants in three separated greenhouses, sited in distinct locations in southern Spain. In all cases, Bemisia tabaci populations were observed on infected plants. The symptomology observed was similar to that caused by whitefly transmitted Cucurbit yellow stunting disorder virus (CYSDV, genus Crinivirus, family Closteroviridae), which is usually found infecting cucumber plants in this geographical area (1). Read More

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Antibody response induced by the BNT162b2 mRNA COVID-19 vaccine in a cohort of health-care workers, with or without prior SARS-CoV-2 infection: a prospective study.

Clin Microbiol Infect 2021 Jul 27. Epub 2021 Jul 27.

Department of Infectious Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria hospital, via Sempreboni 5, 37024 Negrar di Valpolicella, Verona, Italy; Department of Diagnostics and Public Health, University of Verona, Verona, Italy.

Objectives: We assessed the antibody response to BNT162b2 mRNA COVID-19 vaccine in a cohort of health care workers (HCW), comparing subjects with previous SARS-CoV-2 infection and naïve subjects.

Methods: HCW were tested at T0 (day of first dose), T1 (day of second dose), and T2 (2-3 weeks after) for IgG anti nucleocapside protein, IgM anti spike protein and IgG anti receptor binding domain (IgG-RBD-S). The antibody response was compared between 4 main groups: A) Subjects with previous infection and positive antibodies at baseline; B) subjects with same history but negative antibodies; C) subjects with no infection history but positive antibodies; D) naïve subjects. Read More

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Identification of a potent Nrf2 displacement activator among aspirin-containing prodrugs.

Neurochem Int 2021 Jul 27:105148. Epub 2021 Jul 27.

Darby Children's Research Institute, Medical University of South Carolina, Charleston, SC, USA; Department of Pediatrics, Medical University of South Carolina, Charleston, SC, USA; Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA; Department of Drug Discovery, Medical University of South Carolina, Charleston, SC, USA. Electronic address:

Aspirin is a desired leaving group in prodrugs aimed at treatment of neurodegeneration and other conditions. A library of aspirin derivatives of various scaffolds potentially activating Nrf2 has been tested in Neh2-luc reporter assay which screens for direct Nrf2 protein stabilizers working via disruption of Nrf2-Keap1 interaction. Most aspirin prodrugs had a pro-alkylating or pro-oxidant motif in the structure and, therefore, were toxic at high concentrations. Read More

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Inhibition of α-glucosidase by flavonoids of Cymbopogon citratus (DC) Stapf.

J Ethnopharmacol 2021 Jul 27:114470. Epub 2021 Jul 27.

University of Coimbra, Faculty of Pharmacy of University of Coimbra, Coimbra, Portugal; LAQV, REQUIMTE, Faculty of Pharmacy, University of Coimbra, Azinhaga de Santa Comba, 3000-548, Coimbra, Portugal. Electronic address:

Ethnopharmacological Relevance: Leaves extracts from Cymbopogon citratus (DC) Stapf. are widely used in traditional medicine exhibiting several in vivo biological activities, including antidiabetic. Several flavonoids, including aglycones and glycosides, were reported in this plant and previous studies suggested that flavonoids may interact with targets related to diabetes. Read More

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Development of dual crosslinked mumio-based hydrogel dressing for wound healing application: Physico-chemistry and antimicrobial activity.

Int J Pharm 2021 Jul 27:120952. Epub 2021 Jul 27.

Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tr. T. Bati 5678, 76001, Zlin, Czech Republic; Footwear Research Centre, University Institute, Tomas Bata University in Zlin, Nad Ovcirnou IV, 3685, Zlin, Czech Republic; Faculty of Technology, Tomas Bata University in Zlin, Vavrečkova 275, 76001 Zlin, Czech Republic.

In this study, an antimicrobial mumio-based hydrogel dressing was developed for wound healing application. The mechanism of gel formation was achieved via a double crosslink network formation between gelatin (GT) and polyvinyl alcohol (PVA) using polyethylene glycol diglycidyl ether (PEGDE) and borax as crosslinking agents. To enhance the mechanical integrity of the hydrogel matrix, bacterial cellulose (BC) was integrated into the GT-PVA hydrogel to produce a composite gel dressing. Read More

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Effects of new antiseizure drugs on seizure activity and anxiety-like behavior in adult zebrafish.

Toxicol Appl Pharmacol 2021 Jul 27:115655. Epub 2021 Jul 27.

Department of Animal Physiology and Pharmacology, Institute of Biological Sciences, Maria Curie-Sklodowska University, Akademicka 19, PL 20-033 Lublin, Poland. Electronic address:

Several studies with larvae and adult zebrafish have shown that old and new antiseizure drugs (ASDs) produce discrepant results in seizure tests, locomotor activity or anxiety models. In this study, the pentylenetetrazole seizure test (PTZ) was performed to assess the effectiveness of four new ASDs: lamotrigine (LTG), topiramate (TPM), felbamate (FBM), and levetiracetam (LEV) in the subsequent stages of seizures in adult fish. All ASDs were administered intraperitoneally (i. Read More

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External quality assessment in the absence of proficiency testing: a split-sample testing program experience.

Clin Biochem 2021 Jul 27. Epub 2021 Jul 27.

Quality Department. Biomedical Diagnostic Center. Hospital Clínic of Barcelona.

In the field of laboratory medicine, proficiency testing is a vehicle used to improve the reliability of reported results. When proficiency tests are unavailable for a given analyte, an alternative approach is required to ensure adherence to the International Organization for Standardization (ISO) 15189:2012 standard. In this study, we report the results of a split-sample testing program performed as an alternative to a formal PT. Read More

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Meta-analysis of time to extrusion of tympanostomy tubes by tympanic membrane quadrant.

Clin Otolaryngol 2021 Jul 30. Epub 2021 Jul 30.

Department of Otolaryngology Head, Neck and Maxillofacial Surgery, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel, affiliated to the Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.

Objectives: To investigate the published time to extrusion of tympanostomy tubes inserted in the various quadrants of the tympanic membrane.

Methods: Publications were selected by a search with "PubMed", "Embase", and "Web of Science". A meta-analysis of time to extrusion as a function of tympanic membrane quadrant intubation was carried out. Read More

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Multi-Site Evaluation of Immunoassays for Antipsychotic Drug Measurement in Clinical Samples.

J Appl Lab Med 2021 Jul 30. Epub 2021 Jul 30.

Department of Pathology and Laboratory Medicine, Perelman School of Medicine of the University of Pennsylvania, Philadelphia, PA.

Background: Atypical antipsychotic drugs are frequently used in the treatment of serious mental illness (SMI), specifically schizophrenia and bipolar disorder. Adherence to these prescribed drug regimens is a challenge to successful treatment with these drugs. For some of the more common drugs in this class, novel turbidimetric immunoassays have been developed for therapeutic drug monitoring (TDM) to aid in the management of patients prescribed these drugs. Read More

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A methodologically sound survey of Chinese consumers' willingness to participate in courier, express, and parcel companies' green logistics.

PLoS One 2021 30;16(7):e0255532. Epub 2021 Jul 30.

Anhui Vocational College of Electronics and Information Technology, Bengbu, Anhui, China.

The environmental footprint of courier, express, and parcel (CEP) logistics is significant and growing, owing to increased e-commerce. Consumer willingness to participate in the green logistics of CEPs, however, has been understudied. This study addresses this knowledge gap by surveying 155 Chinese consumers about their willingness to participate in CEP green logistics. Read More

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