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Salidroside Attenuates Airway Inflammation and Remodeling via the miR-323-3p/SOCS5 Axis in Asthmatic Mice.

Int Arch Allergy Immunol 2021 Dec 2:1-11. Epub 2021 Dec 2.

First Clinical Medical College, Yunnan University of Chinese Medicine, Kunming, China.

Introduction: Salidroside (Sal) a bioactive component extracted from Rhodiola rosea is remarkable for its anti-asthmatic effects. The study aimed to explore the molecular mechanism of Sal in airway inflammation and remodeling in asthmatic mice and provide a novel theoretical basis for asthma treatment.

Methods: An asthmatic mouse model was established via ovalbumin (OVA) treatment, followed by injection of Sal and transfection of miR-323-3p-mimic and sh- suppressor of cytokine signaling 5 (SOCS5). Read More

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December 2021

Benzodiazepines safeguards nerve cells from the toxicity of lidocaine via miR-133a-3p/EGFR pathway.

Transpl Immunol 2021 Nov 29:101510. Epub 2021 Nov 29.

Anesthesiology Department, The Forth people's hospital of Jinan, China.

Background: Lidocaine was an anesthetic commonly used for analgesia, but the neurotoxicity could not be ignored. However, benzodiazepines could alleviate the toxicity when combined with other drugs.

Purpose: To explore the molecular mechanism of benzodiazepines in protecting nerve cells after the induction of lidocaine. Read More

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November 2021

The role of size-controlled CeO nanoparticles in enhancing the stability and photocatalytic performance of ZnO in natural sunlight exposure.

Chemosphere 2021 Nov 29:133092. Epub 2021 Nov 29.

Centre of Excellence in Environmental Studies (CEES), King Abdulaziz University, Jeddah, 21589, Saudi Arabia; National Centre for Physics, Quaid-e-Azam University, Islamabad, 44000, Pakistan. Electronic address:

In order to enhance the photocatalytic performance and stability, the various proportions of the size controlled cerium oxide (CeO) nanoparticles were dispersed at the pre-synthesized ZnO. Although, the expected dual absorption onsets, probably due to the diminutive difference between the bandgaps of CeO (∼2.9 eV) and ZnO (∼3. Read More

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November 2021

Simultaneous Evaluations of pH and Enzyme Activity with a CEST MRI Contrast Agent.

ACS Sens 2021 Dec 2. Epub 2021 Dec 2.

Department of Cancer Systems Imaging, University of Texas MD Anderson Cancer Center, Houston, Texas 77054, United States.

The extracellular tumor microenvironment of many solid tumors has high acidosis and high protease activity. Simultaneously assessing both characteristics may improve diagnostic evaluations of aggressive tumors and the effects of anticancer treatments. Noninvasive imaging methods have previously been developed that measure extracellular pH or can detect enzyme activity using chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI). Read More

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December 2021

Integrative investigation of the TF-miRNA coregulatory network involved in the inhibition of breast cancer cell proliferation by resveratrol.

FEBS Open Bio 2021 Dec 2. Epub 2021 Dec 2.

Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education, First Hospital, Jilin University, Changchun, 130021.

Resveratrol is a polyphenol with antiaging and anticancer effects. Most previous studies used a single analysis to determine the key functions of resveratrol in inhibiting cancer progression. However, most of the signal transmission pathways in biological processes are multilevel. Read More

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December 2021

Lnc-hipk1 inhibits mouse adipocyte apoptosis as a sponge of miR-497.

Biofactors 2021 Dec 2. Epub 2021 Dec 2.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.

Noncoding RNAs (ncRNAs) such as microRNAs (miRNAs), long noncoding RNAs (lncRNA), and circular RNAs are closely related to the biological processes related to obesity. As a miRNA that widely present in different cell types, miR497 is proved to be involved in cell development. However, research on the role of miR-497 as a key factor in regulating the development of adipocytes is still in gap. Read More

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December 2021

Can the dual-rating regulation improve the rating quality of Chinese corporate bonds?

Authors:
Xiangyun Zhou

PLoS One 2021 2;16(12):e0259759. Epub 2021 Dec 2.

Shenzhen Institute of Information Technology, Shenzhen, China.

We developed a dual-reputational rating shopping model to introduce public and institutional reputations. Investor's and regulator's penalty rates are described as public and institutional reputations, respectively. We achieved the available conditions of single-rating and dual-rating regulations to prevent rating inflation in this model. Read More

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December 2021

Understanding the usability issues in contact management of illiterate and semi-literate users.

PLoS One 2021 2;16(12):e0259719. Epub 2021 Dec 2.

Department of Computer Science, FAST National University of Computer and Emerging Sciences, Islamabad, Pakistan.

The effective utilization of a communication channel like calling a person involves two steps. The first step is storing the contact information of another user, and the second step is finding contact information to initiate a voice or text communication. However, the current smartphone interfaces for contact management are mainly textual; which leaves many emergent users at a severe disadvantage in using this most basic functionality to the fullest. Read More

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December 2021

Digital transformation of the mobile connected pharmacy: a first step toward community pharmacy 5.0.

Inform Health Soc Care 2021 Dec 2:1-14. Epub 2021 Dec 2.

Farmácia Saúde, Figueira Da Foz, Portugal.

Community pharmacies have made significant advances in digital technology; however, mobile systems are only emerging in this sector and mostly focusing patient-centric connections. This study reveals a case of digital transformation in a mobile connected pharmacy, balancing efficient pharmaceutical services and digital innovation. A mobile connected pharmacy solution (mPharmaCare) is developed for a community of near 100. Read More

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December 2021

Long non-coding RNA LINC01194 promotes the inflammatory response and apoptosis of LPS-treated MLE 12 cells through the miR-203a-3p /MIP-2 axis.

Can J Physiol Pharmacol 2021 Dec 2. Epub 2021 Dec 2.

Xincheng Branch of Zaozhuang Municipal Hospital, Zaozhuang, China;

Acute lung injury (ALI) induced by bacteria LPS is characterized by the upregulation of the apoptosis rate of tissue cells and aggravation of inflammatory response. Although many studies have focused on the pathogenesis of this disease, its mechanism remains unknown. This study examined the regulatory role of long non-coding RNA (lncRNA) LINC01194 in the progression of ALI through various bioinformatics analyses and experimental work, including ELISA assay, dual-luciferase reporter assay, biotinylated RNA pull-down assay, and western blot analysis. Read More

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December 2021

A Photoresponsive and Metal-Organic Framework Encapsulated DNA Tetrahedral Entropy-Driven Amplifier for High-Performance Imaging Intracellular MicroRNA.

Anal Chem 2021 Dec 2. Epub 2021 Dec 2.

Hubei Province Key Laboratory of Occupational Hazard Identification and Control, School of Public Health, Medical College, Wuhan University of Science and Technology, Wuhan 430065, People's Republic of China.

The further development of high-performance fluorescent biosensors to image intracellular microRNAs is beneficial to cancer medicine. By virtue of the need for enzymes and hairpin DNA probes, the entropy-driven reaction-assisted signal amplification strategy has shown an enormous potential to accomplish this task. Nevertheless, this good option still meets with poor biostability, low cell uptake efficiency, and unsatisfactory accuracy. Read More

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December 2021

Exosomal lnc-AFTR as a novel translation regulator of FAS ameliorates Staphylococcus aureus-induced mastitis.

Biofactors 2021 Dec 2. Epub 2021 Dec 2.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

Although the specific expression of long noncoding RNA (lncRNA) in mastitis tissue has been reported, few studies have involved the differential expression of lncRNA in mastitis exosomes (Exo) and its mechanism and function. We screened an lncRNA associated with FAS translational regulation (lnc-AFTR) through exosomal RNA sequencing, and clarified its function and molecular mechanism. Lnc-AFTR is markedly downregulated in Staphylococcus aureus-Exo and S. Read More

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December 2021

Ropivacaine Retards the Viability, Migration, and Invasion of Choriocarcinoma Cells by Regulating the Long Noncoding RNA OGFRP1/MicroRNA-4731-5p/HIF3A Axis.

Mol Biotechnol 2021 Dec 2. Epub 2021 Dec 2.

Department of Anaesthesiology, Fudan University Shanghai Cancer Center, No. 270, Dong'an Road, Shanghai City, 200032, China.

Choriocarcinoma is an aggressive gestational trophoblastic neoplasm. This study attempted to explore the biological functions and underlying mechanisms by which ropivacaine restrains the progression of choriocarcinoma. The expression of long noncoding RNA OGFRP1, microRNA-4731-5p (miR-4731-5p), and HIF3A in choriocarcinoma cells was assessed by qRT-PCR. Read More

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December 2021

MiRNA-339-5p promotes isoproterenol-induced cardiomyocyte hypertrophy by targeting VCP to activate the mTOR signaling.

Cell Biol Int 2021 Dec 2. Epub 2021 Dec 2.

Department of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China.

MicroRNAs (miRNAs) regulate multiple biological processes and participate in various cardiovascular diseases. This study aims to investigate the role of miR-339-5p in cardiomyocyte hypertrophy and the involved mechanism. Neonatal rat cardiomyocytes (NRCMs) were cultured and stimulated with isoproterenol (ISO). Read More

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December 2021

MiR-125a-3p inhibits cell proliferation and inflammation responses in fibroblast-like synovial cells in rheumatoid arthritis by mediating the Wnt/β-catenin and NF-κB pathways via targeting MAST3.

J Musculoskelet Neuronal Interact 2021 Dec;21(4):560-567

Department of Rheumatology, The Second Hospital of Shandong University, Shandong Province, China.

Objectives: To explore the role and mechanism of miR-125a-3p in rheumatoid arthritis (RA) progression.

Methods: The RA-tissues and fibroblast-like synovial cells in rheumatoid arthritis (RA-FLS) were used in this study. qRT-PCR, western blot and ELISA assay were performed to detect the expression levels of IL-6, IL-β and ΤΝF-α. Read More

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December 2021

Body composition and maximal exercise capacity after heart transplantation.

ESC Heart Fail 2021 Dec 2. Epub 2021 Dec 2.

Service de Cardiologie, Département Coeur-Vaisseaux, Lausanne University Hospital (CHUV) and University of Lausanne (UNIL), Lausanne, Switzerland.

Aims: Maximal exercise capacity as measured by peak oxygen consumption (pVO ) in cardiopulmonary exercise testing (CPET) of heart transplant recipients (HTR) is limited to a 50-70% level of healthy age-matched controls. This study investigated the relationship between body composition and pVO during the first decade post-transplant.

Methods And Results: Body composition was determined by dual-energy X-ray absorptiometry (DXA) and pVO by CPET in 48 HTR (n = 38 males; mean age 51 ± 12 years). Read More

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December 2021

Circ_0059354 aggravates the progression of papillary thyroid carcinoma by elevating ARFGEF1 through sponging miR-766-3p.

J Endocrinol Invest 2021 Dec 2. Epub 2021 Dec 2.

Department of General Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, No. 157, Xiwu Road, Xi'an, 710004, Shaanxi, China.

Background: Circular RNAs (circRNAs) have been identified as vital players in tumors, including papillary thyroid carcinoma (PTC). The purpose of this study is to explore the functions of circ_0059354 on PTC development.

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to examine the levels of circ_0059354, microRNA-766-3p (miR-766-3p) and ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1). Read More

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December 2021

Elsholtzia rugulosa: Phytochemical Profile and Antioxidant, Anti-Alzheimer's Disease, Antidiabetic, Antibacterial, Cytotoxic and Hepatoprotective Activities.

Plant Foods Hum Nutr 2021 Dec 1. Epub 2021 Dec 1.

Clinical Medical College, Yangzhou University, Yangzhou, 225001, China.

Elsholtzia rugulosa Hemsl., a species of the Labiatae family, has a long history of use as a honey plant, herbal tea, and folk medicine in China. However, little is known about its composition and biological activities. Read More

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December 2021

No effect of maternal calcium intake and bone resorption during pregnancy on offspring bone mineral density at age 5 years.

Osteoporos Int 2021 Dec 1. Epub 2021 Dec 1.

UCD Perinatal Research Centre, School of Medicine, University College Dublin, National Maternity Hospital, Dublin, Ireland.

Maternal nutrition during pregnancy plays a role in offspring bone health. In a prospective cohort study, offspring bone mineral density at 5 years was not associated with maternal calcium intake or maternal bone resorption during pregnancy.

Purpose: Suboptimal bone mineral density in childhood can result in osteoporosis later in life. Read More

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December 2021

Degree of conversion of resin-cements (light-cured/dual-cured) under different thicknesses of vitreous ceramics: systematic review.

J Prosthodont Res 2021 Dec 1. Epub 2021 Dec 1.

Facultad de Odontología, Universidad de Antioquia. Medellín, Colombia.

Purpose: This systematic review synthesized and analyzed the scientific evidence on the degree of conversion (DC) obtained by Fourier-transform infrared spectroscopy (FTIR) of light-cured and dual-cured resinous cements, photopolymerized under different thicknesses of vitreous ceramics.

Study Selection: The study protocol of this systematic review was registered at the International Prospective Register of Systematic Reviews (PROSPERO) (CRD42017069319). A comprehensive search (PubMed/MEDLINE, Scopus, EMBASE, and LILACS) was performed for papers including an in vitro design and indexed from January 2007 to December 2020 according to the study purposes. Read More

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December 2021

CircEAF2 counteracts Epstein-Barr virus-positive diffuse large B-cell lymphoma progression via miR-BART19-3p/APC/β-catenin axis.

Mol Cancer 2021 Dec 1;20(1):153. Epub 2021 Dec 1.

Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 197 Rui Jin Er Road, Shanghai, 200025, China.

Background: Epstein-Barr virus (EBV) represents an important pathogenic factor of lymphoma and is significantly associated with poor clinical outcome of diffuse large B-cell lymphoma (DLBCL). Circular RNAs (circRNAs) play an essential role in lymphoma progression. However, the underlying mechanism of circRNA on DLBCL progression related to EBV remains largely unknown. Read More

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December 2021

Stroke of antiplatelet and anticoagulant therapy in patients with coronary artery disease: a meta-analysis of randomized controlled trials.

BMC Cardiovasc Disord 2021 Dec 1;21(1):574. Epub 2021 Dec 1.

Anzhen Hospital, Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Disease, The Key Laboratory of Remodeling-Related Cardiovascular Disease, Ministry of Education, Beijing, China.

Background: We performed a meta-analysis sought to investigate the risk of stroke with antiplatelet and anticoagulant therapies among patients with coronary artery disease (CAD).

Methods: We searched PubMed, EMBASE, and Cochrane Library for randomized controlled trials from January 1995 to March 2020. Studies were retrieved if they reported data of stroke for patients with CAD and were randomized to receive intensive versus conservative antithrombotic therapies, including antiplatelet and oral anticoagulant (OAC). Read More

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December 2021

Circular RNA circFLNA inhibits the development of bladder carcinoma through microRNA miR-216a-3p/BTG2 axis.

Bioengineered 2021 Dec;12(2):11376-11389

Department of Urology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.

Recent studies have shown that circular RNA circFLNA is abnormally expressed in a variety of malignant tumors, but its role and mechanism in bladder carcinoma (BCa) are still unclear. The present paper aims to contribute to research on the effects and mechanism of circFLNA on the malignant phenotype of BCa. In this study, the expressions of circFLNA, miR-216a-3p and BTG2 in BCa and BCa cells (EJ, T24, 5637, TCC-SUP) were detected by qRT-PCR. Read More

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December 2021

Moyamoya Syndrome in an Infant with Aicardi-Goutières and Williams Syndromes: A Case Report.

Neuropediatrics 2021 Dec 1. Epub 2021 Dec 1.

Department of Pediatrics, Division of Pediatric Critical Care Medicine, Children's Hospital, London Health Sciences Centre, London, Ontario, Canada.

Stroke in infancy is a rare phenomenon but can lead to significant long-term disability. We present the story of a 6-month-old Old Order Amish infant with underlying Williams syndrome, a rare neurodevelopmental disorder caused by a microdeletion, encompassing the elastin gene that produces abnormalities in elastic fibers of the lungs and vessels. This infant presented with lethargy, irritability, and a new-onset generalized tonic-clonic seizure. Read More

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December 2021

Transcriptional activation and regulation of urokinase plasminogen activator inducted by LPS through MyD88 independent pathway in rat sertoli cells.

Folia Histochem Cytobiol 2021 Dec 1. Epub 2021 Dec 1.

Department of Human Anatomy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Introduction: Urokinase plasminogen activator (uPA) is a serine protease and it also demonstrates proinflammatory properties. We thus hypothesized that uPA is involved in immunity of Sertoli cells in the rat testis.

Materials And Methods: The uPA gene(PLAU) promoter was cloned by RT-PCR and the transcriptional activation of core domain was screened by using Dual-Luciferase Reporter Assay System. Read More

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December 2021

A dual-channel colorimetric and fluorescent sensor for the rapid and ultrasensitive detection of kanamycin based on gold nanoparticles-copper nanoclusters.

Anal Methods 2021 Dec 1. Epub 2021 Dec 1.

School of Ecological and Environmental Sciences, Shanghai Engineering Research Center of Biotransformation of Organic Solid Waste, Shanghai Key Lab for Urban Ecological Process and Eco-Restoration, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.

In this work, a dual-channel assay was constructed for the colorimetric and fluorescent detection of kanamycin (KAN) based on gold nanoparticles (Au NPs) and copper nanoclusters (Cu NCs). Initially, the fluorescence of Cu NCs was quenched by 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (AHMT)-functionalized Au NPs due to the inner filter effect (IFE). The existence of KAN acted as a molecular bridge to interact with AHMT hydrogen bonds and induced the aggregation of AHMT-Au NPs, leading to a change in the color of the gold colloidal solution from reddish-violet to blue within 2 min. Read More

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December 2021

Convergent Evidence Supports TH2LCRR as a Novel Asthma Susceptibility Gene.

Am J Respir Cell Mol Biol 2021 Dec 1. Epub 2021 Dec 1.

Shaanxi Normal University, 12401, College of Life Sciences, Xi'an, China;

Asthma is a common complex disease with apparent genetic predispositions, and previous genome-wide association studies suggest that rs1295686 within the IL13 (interleukin 13) gene is significantly associated with asthma. Analysis of the data provided by the 1000 Genomes Project indicated that there are additional four SNPs in nearly complete linkage disequilibrium with rs1295686 in Caucasians. However, the causal SNPs and the associated mechanism remain unclear. Read More

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December 2021

A Dual-Antigen SARS-CoV-2 Serological Assay Reflects Antibody Avidity.

J Clin Microbiol 2021 Dec 1:JCM0226221. Epub 2021 Dec 1.

Department of Parasitology.

Serial antibody measurements using an array of SARS-CoV-2 immunoassays have demonstrated differing kinetics among assay platforms (1).…. Read More

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December 2021

genetic polymorphism and incidence of in-stent restenosis in patients on clopidogrel: a matched case-control study.

Drug Metab Pers Ther 2021 Jan 1. Epub 2021 Jan 1.

Department of Pharmacy, Faculty of Medicine and Surgery, University of Malta, Msida, Malta.

Objectives: The cytochrome P450 2C19 () genetic polymorphism is associated with reduced clopidogrel bioactivation, increasing the risk of atherothrombotic complications after percutaneous coronary intervention (PCI). In-stent restenosis (ISR) is a complication that limits the long-term prognosis of PCI. The aim was to investigate the association between presence of the allele and ISR within one-year after PCI in patients prescribed dual antiplatelet therapy with aspirin and clopidogrel. Read More

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January 2021

Interfering TUG1 Attenuates Cerebrovascular Endothelial Apoptosis and Inflammatory injury After Cerebral Ischemia/Reperfusion via TUG1/miR-410/FOXO3 ceRNA Axis.

Neurotox Res 2021 Dec 1. Epub 2021 Dec 1.

Department of Neurology, Henan Provincial People's Hospital, Zhengzhou, 450003, Henan, China.

Background Emerging studies illustrate that long non-coding RNA TUG1 (TUG1) participates in neuron death after ischemia. However, the role of TUG1 in cerebral ischemia/reperfusion (CI/R) injury through cerebrovascular pathology was undetermined yet. Methods Expression of TUG1, miRNA-410-3p (miR-410), and forkhead box O3 (FOXO3) was detected by RT-qPCR and western blot. Read More

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December 2021