683,458 results match your criteria assay developed


LC-MS/MS method for simultaneous quantification of the first-line anti-tuberculosis drugs and six primary metabolites in patient plasma: Implications for therapeutic drug monitoring.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Oct 14;1185:122986. Epub 2021 Oct 14.

Latvian Biomedical Research and Study Centre, Ratsupites Street 1, k-1, Riga, LV1067, Latvia; Riga Stradins University, Dzirciema Street 16, Riga, LV1007, Latvia. Electronic address:

The pharmacokinetic profiling of drug substances and corresponding metabolites in the biological matrix is one of the most informative tools for the treatment efficacy assessment. Therefore, to satisfy the need for comprehensive monitoring of anti-tuberculosis drugs in human plasma, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous quantification of first-line anti-tuberculosis drugs (ethambutol, isoniazid, pyrazinamide, and rifampicin) along with their six primary metabolites. Simple single-step protein precipitation with methanol was chosen as the most convenient sample pre-treatment method. Read More

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October 2021

Determination of morphine, codeine, and thebaine concentrations from poppy seed tea using magnetic carbon nanotubes facilitated dispersive micro-solid phase extraction and GC-MS analysis.

Forensic Sci Int 2021 Oct 12;329:111052. Epub 2021 Oct 12.

Department of Forensic Science, Sam Houston State University, 1003 Bowers Blvd, Huntsville, TX, USA. Electronic address:

With tightening enforcement and restrictions amid the opioid epidemic, poppy seed tea is consumed as an alternative to mitigate the withdrawal symptoms or as a home remedy to relieve pain and stress. Previously published studies suggested the potential danger of consuming tea brewed with a moderate to a large amount of poppy seed. In this study, the effects of small quantity and repeat brewing on opiate concentrations were evaluated. Read More

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October 2021

Prognostic capacity of inferior vena cava diameter for severe postpartum hemorrhage.

Eur J Obstet Gynecol Reprod Biol 2021 Oct 16;267:6-10. Epub 2021 Oct 16.

Department of Gynecology and Obstetrics, Aksaray University Education and Research Hospital, Aksaray, Turkey. Electronic address:

Purpose: This study sought to evaluate whether or not the sonographic measurement of the inferior vena cava (IVC) diameter predicts severe bleeding in women with postpartum hemorrhage (PPH). It further compared the efficacy of the IVC diameter, shock index, and serum lactate levels in this prediction.

Methods: This prospective study included female patients with ≥500 mL of bleeding after vaginal delivery between September 1, 2019 and May 31, 2021. Read More

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October 2021

Assessing the differential sensitivities of wave-CAIPI ViSTa myelin water fraction and magnetization transfer saturation for efficiently quantifying tissue damage in MS.

Mult Scler Relat Disord 2021 Oct 4;56:103309. Epub 2021 Oct 4.

McConnell Brain Imaging Centre, Montreal Neurological Institute and Hospital, Montreal, QC, Canada; Department of Biomedical Engineering, McGill University, Montreal, QC, Canada; Department of Neurology and Neurosurgery, McGill University, Montreal, QC, Canada.

Background: Wave-CAIPI Visualization of Short Transverse relaxation time component (ViSTa) is a recently developed, short-T-sensitized MRI method for fast quantification of myelin water fraction (MWF) in the human brain. It represents a promising technique for the evaluation of subtle, early signals of demyelination in the cerebral white matter of multiple sclerosis (MS) patients. Currently however, few studies exist that robustly assess the utility of ViSTa MWF measures of myelin compared to more conventional MRI measures of myelin in the brain of MS patients. Read More

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October 2021

A large margin piecewise linear classifier with fusion of deep features in the diagnosis of COVID-19.

Comput Biol Med 2021 Oct 11;139:104927. Epub 2021 Oct 11.

Department of Business Information Systems University of Applied Sciences Western Switzerland, Sierre (HES SO), Switzerland. Electronic address:

The world has experienced epidemics of coronavirus infections several times over the last two decades. Recent studies have shown that using medical imaging techniques can be useful in developing an automatic computer-aided diagnosis system to detect pandemic diseases with high accuracy at an early stage. In this study, a large margin piecewise linear classifier was developed to diagnose COVID-19 compared to a wide range of viral pneumonia, including SARS and MERS, using chest x-ray images. Read More

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October 2021

The effect of dental restoration geometry and material properties on biomechanical behaviour of a treated molar tooth: A 3D finite element analysis.

J Mech Behav Biomed Mater 2021 Oct 14;125:104892. Epub 2021 Oct 14.

School of Mechanical and Manufacturing Engineering, University of New South Wales, Sydney, NSW, 2052, Australia; ARC Centre for Automated Manufacture of Advanced Composites, School of Mechanical and Manufacturing Engineering, University of New South Wales, Sydney, NSW, Australia.

Objectives: To test the hypothesis that restoration of class II mesio-occlusal-distal (MOD) cavities can be strengthened through judicious choice of restoration geometry and material properties.

Methods: An intact extracted human maxillary molar tooth was digitized, segmented, reconstructed, and four 3D restored tooth models were developed with four different restoration geometries: one straight, one single-curved, and two double-curved. Stress analysis was conducted for representative loading using finite element analysis, and maximum principal stresses were determined at the dentine-enamel and restoration-enamel junctions. Read More

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October 2021

A highly polymorphic panel of 40-plex microhaplotypes for the Chinese Han population and its application in estimating the number of contributors in DNA mixtures.

Forensic Sci Int Genet 2021 Oct 8;56:102600. Epub 2021 Oct 8.

Department of Forensic Medicine, Nanjing Medical University, Nanjing, Jiangsu, 211166, PR China; Key Laboratory of Targeted Intervention of Cardiovascular Disease, Collaborative Innovation Center for Cardiovascular Disease Translational Medicine, Nanjing Medical University, Nanjing, Jiangsu, 211166, PR China. Electronic address:

Microhaplotypes (MHs) have great potential in multiple forensic applications and have proven to be promising markers in complex DNA mixture analysis. In this study, we developed a multiplex panel of 40 highly polymorphic MHs for the Chinese Han population, evaluated its forensic values, and explored its application in predicting the number of contributors (NOCs) in DNA mixtures. The panel consisted of 20 newly proposed loci and 20 previously reported loci with lengths spanning less than 120 bp. Read More

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October 2021

Factors predicting morbidity in surgically-staged high-risk endometrial cancer patients.

Eur J Obstet Gynecol Reprod Biol 2021 Sep 28;266:169-174. Epub 2021 Sep 28.

Gynecological Oncology Unit, Fondazione IRCCS Istituto Nazionale dei Tumori di Milano, Milano, Italy.

Objective: To investigate factors predicting the risk of developing 90-day postoperative complications and lymphatic-specific morbidity in patients undergoing surgical staging for high-risk endometrial cancer.

Methods: This is a multi-institutional retrospective cohort study. Patients affected by apparent early-stage high-risk endometrial cancer (endometrioid FIGO grade 3 with deep myometrial invasion and non-endometrioid endometrial cancer) undergoing surgical staging between 2007 and 2019. Read More

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September 2021

Evidence for retained spike-binding and neutralizing activity against emerging SARS-CoV-2 variants in serum of COVID-19 mRNA vaccine recipients.

EBioMedicine 2021 Oct 20;73:103626. Epub 2021 Oct 20.

Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA; Department of Pathology, Molecular and Cell Based Medicine Icahn School of Medicine at Mount Sinai, New York, NY, USA; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA; The Global Health and Emerging Pathogen Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. Electronic address:

Background: Highly efficacious vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been developed. However, the emergence of viral variants that are more infectious than the earlier SARS-CoV-2 strains is concerning. Several of these viral variants have the potential to partially escape neutralizing antibody responses, warranting continued immune-monitoring. Read More

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October 2021

Novel 5-fluorouracil sensitizers for colorectal cancer therapy: Design and synthesis of S1P receptor 2 (S1PR2) antagonists.

Eur J Med Chem 2021 Oct 15;227:113923. Epub 2021 Oct 15.

Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, 266003, Qingdao, China. Electronic address:

Sphingosine-1-phosphate receptor 2 (S1PR2) has been identified as a brand-new GPCR target for designing antagonists to reverse 5-FU resistance. We herein report the structural optimization and structure-activity relationship of JTE-013 derivatives as S1PR2 antagonists. Compound 9d was the most potent S1PR2 antagonist (K = 34. Read More

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October 2021

Cognitive Assessment scale for Stroke Patients (CASP): a multicentric validation study.

Ann Phys Rehabil Med 2021 Oct 20:101594. Epub 2021 Oct 20.

Centre d'Investigation Clinique CIC 1432 and University of Bourgogne-Franche-Comté, France.

Background: The Mini Mental State Examination and Montreal Cognitive Assessment are commonly used as short screening batteries for assessing cognitive impairment after stroke. However, aphasia or hemispatial neglect may interfere with the results. For this reason, we developed the Cognitive Assessment scale for Stroke Patients (CASP), which takes these conditions into consideration and previously demonstrated its superiority over these scales in terms of feasibility. Read More

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October 2021

Development of a Preoperative Clinical Risk Assessment Tool for Postoperative Complications After Hysterectomy.

J Minim Invasive Gynecol 2021 Oct 20. Epub 2021 Oct 20.

the Department of Obstetrics and Gynecology, University of Michigan, 1500 E. Medical Center Dr., Ann Arbor, MI 48109 USA. Electronic address:

Study Objective: To develop a preoperative risk assessment tool that quantifies the risk of postoperative complications within 30 days of hysterectomy.

Design: Retrospective analysis.

Setting: Michigan Surgical Quality Collaborative hospitals. Read More

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October 2021

Enhanced Recovery After Surgery (ERAS) for Open Transforaminal Lumbar Interbody Fusion: A Retrospective Propensity-Matched Cohort Study.

Spine J 2021 Oct 20. Epub 2021 Oct 20.

College of Medicine, University of Florida, Gainesville, FL, USA; Department of Anesthesiology, University of Florida, Gainesville, FL, USA.

Background: The enhanced recovery after surgery (ERAS) protocol is a multidisciplinary, multimodal approach which has been shown to facilitate recovery of physiological function, and reduce postoperative pain, complication rates, and length of stay without adversely affecting readmission rates. Design and implementation of ERAS protocols in the recent spine surgery literature has primarily focused on patients undergoing minimally invasive lumbar surgery. However, conventional open transforaminal lumbar interbody fusion (TLIF) remains a common procedure and to date there are no studies assessing an ERAS protocol in this patient population. Read More

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October 2021

Oral administration of berberine limits post-traumatic osteoarthritis development and associated pain via AMP-activated protein kinase (AMPK) in mice.

Osteoarthritis Cartilage 2021 Oct 20. Epub 2021 Oct 20.

VA San Diego Healthcare System, San Diego, USA; Department of Medicine, University of California San Diego, La Jolla, USA. Electronic address:

Objective: We investigated the effect of berberine, a natural plant product that can activate AMP-activated protein kinase (AMPK), on OA development and associated pain in mice.

Design: Human primary knee chondrocytes were utilized to investigate how AMPK is activated by berberine. Both global knockout (KO) of AMPKα1 and congenic wild type (WT) mice were subjected to the post-traumatic OA through destabilization of medial meniscus (DMM) surgery. Read More

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October 2021

Changes in Anesthesia Can Reduce Periprocedural Urinary Retention After EVAR.

Ann Vasc Surg 2021 Oct 20. Epub 2021 Oct 20.

Northwestern Feinberg School of Medicine, Division of Vascular Surgery, Surgery Department, 676 N St. Clair Street, Suite 650, Chicago, Illinois 60611.

Objective: Enhanced recovery after surgery (ERAS) programs provide a streamlined approach for expedient post-operative care of high-volume procedures. Endovascular aortic repair (EVAR) has become standard treatment for abdominal aortic aneurysms and implementation of an early recovery program is warranted. Post-operative urinary retention (POUR) remains a problem lending to longer hospital stays and patient discomfort. Read More

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October 2021

Predictive factors of surgical complications in the first year following kidney transplantation.

Ann Vasc Surg 2021 Oct 20. Epub 2021 Oct 20.

Department of Vascular Surgery, Lausanne University Hospital (CHUV) and University of Lausanne, Suisse. Electronic address:

Background: In the recent years, an increased use of marginal donors and grafts and a growing prevalence of peripheral arterial disease in the recipients have been observed. Meanwhile, the open surgical technique for kidney transplantation has not changed. The aim of this study is to analyze all surgical complications occurring in the first year after kidney transplant and to determine potential predictive risk factors. Read More

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October 2021

A green initiative for oiled sand cleanup using chitosan/rhamnolipid complex dispersion with pH-stimulus response.

Chemosphere 2021 Oct 20:132628. Epub 2021 Oct 20.

Fisheries and Oceans Canada, Ecosystem Science, Ottawa, ON, K1A 0E6, Canada.

The released oil can affect the vulnerable shoreline environment if the oil spills happen in coastal waters. The stranded oil on shorelines is persistent, posing a long-term influence on the intertidal ecosystem after weathering. Therefore, shoreline cleanup techniques are required to remove the oil from the shoreline environment. Read More

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October 2021

Layered filter paper-silver nanoparticle-ZIF-8 composite for efficient multi-mode enrichment and sensitive SERS detection of thiram.

Chemosphere 2021 Oct 20:132635. Epub 2021 Oct 20.

College of Chemistry and Chemical Engineering & Analytical and Testing Centre, Jiangxi Normal University, Nanchang, Jiangxi, People's Republic of China.

A SERS substrate FP/Ag/ZIF-8 composed of filter paper (FP), silver nanoparticles (AgNPs) and zeolitic imidazolate framework (ZIF-8) film arranged in a layered structure was developed for sensitive detection of pesticide thiram in various samples. Roles of these components in analyte adsorption and Raman signal enhancement were studied using a pesticide intermediate 4-Aminothiophenol (4-ATP) as the probe. The substrate showed high adsorption and optimized SERS response with thick metal organic framework (MOF) coating (125 nm), which is different from previous reported plasmonic particle-MOF composite substrate, where thinnest MOF coating produced the strongest SERS signal. Read More

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October 2021

Improved methods to characterize the length and quantity of highly unstable PolySialic acids subject category: (Carbohydrates, chromatographic techniques).

Anal Biochem 2021 Oct 20:114426. Epub 2021 Oct 20.

Department of Cellular & Molecular Medicine, Center for Academic Research and Training in Anthropogeny (CARTA), Glycobiology Research and Training Center (GRTC), University of California, San Diego, La Jolla, CA, USA. Electronic address:

Polysialic acid (polySia) is a linear homopolymer of α2-8-linked sialic acids that is highly expressed during early stages of mammalian brain development and modulates a multitude of cellular functions. While degree of polymerization (DP) can affect such functions, currently available methods do not accurately characterize this parameter, because of the instability of the polymer. We developed two improved methods to characterize the DP and total polySia content in biological samples. Read More

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October 2021

SARS-CoV-2 infection among hospitalized pregnant women and impact of different viral strains on COVID-19 severity in Italy: a national prospective population-based cohort study.

BJOG 2021 Oct 23. Epub 2021 Oct 23.

National Centre for Disease Prevention and Health Promotion, Istituto Superiore di Sanità - Italian National Institute of Health, Viale Regina Elena 299, 00161, Rome, Italy.

Objective: The primary aim of this paper was to describe SARS-CoV-2 infection among pregnant women during the Wild-type and the Alpha period in Italy, the secondary aim was to compare the impact of the virus variants on the severity of maternal and perinatal outcomes.

Design: National population-based prospective cohort study.

Setting: 315 Italian maternity hospitals. Read More

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October 2021

Cormatrix® for vessel reconstruction in paediatric cardiac surgery-a word of caution.

Interact Cardiovasc Thorac Surg 2021 Oct 23. Epub 2021 Oct 23.

Division of Cardiac Surgery, Department of Surgery, Pediatric Heart Center Vienna, Medical University Vienna, Vienna, Austria.

Objectives: The aim of this retrospective study was to determine if Cormatrix® (CM) represents a safe alternative to conventional patch materials used in congenital heart surgery.

Methods: A total of 57 paediatric patients who underwent cardiac surgery using an Extracellular Matrix Bioscaffold (CM) were categorized into 4 groups according to the patch implant location. Patch-related complications and reintervention rates were analysed. Read More

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October 2021

Standardized Workflow for Precise Mid- and High-Throughput Proteomics of Blood Biofluids.

Clin Chem 2021 Oct 23. Epub 2021 Oct 23.

Smidt Heart Institute, Advanced Clinical Biosystems Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.

Background: Accurate discovery assay workflows are critical for identifying authentic circulating protein biomarkers in diverse blood matrices. Maximizing the commonalities in the proteomic workflows between different biofluids simplifies the approach and increases the likelihood for reproducibility. We developed a workflow that can accommodate 3 blood-based proteomes: naive plasma, depleted plasma and dried blood. Read More

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October 2021

Structural valve deterioration of the Labcor Dokimos aortic prosthesis: a single-centre experience.

Interact Cardiovasc Thorac Surg 2021 Oct 23. Epub 2021 Oct 23.

Division of Cardiology, American University of Beirut Medical Center, Beirut, Lebanon.

Objectives: The goal of this study was to assess the performance and incidence of the deterioration of the Labcor Dokimos bioprosthetic aortic valve.

Methods: We performed a retrospective medical chart review of 116 patients who underwent surgical aortic valve replacement with the Labcor Dokimos aortic valve between 2010 and 2018. Abstracted data included patient demographic and echocardiographic data. Read More

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October 2021

PERINATAL CENTRILOBULAR HEPATIC NECROSIS IN GIANT PANDAS (): A RETROSPECTIVE STUDY.

J Zoo Wildl Med 2021 Sep;52(3):926-938

Department of Wildlife Health Sciences, Smithsonian's National Zoo and Conservation Biology Institute, MRC 5501 Veterinary Hospital, Washington, D.C., USA 20013-7012.

Between 1983 and 2012, six giant panda cubs () born at a zoological institution were stillborn or died between the ages of 3 and 200 h. Two of the six cubs had panhepatic centrilobular hepatic necrosis (CHN), granulocytic extramedullary hematopoiesis (GEM), positive liver culture for species, and terminal liver failure. Another low-weight cub was administered oxygen therapy immediately after birth and developed hyaline membranes in air spaces and hepatic necrosis restricted to the hilar region. Read More

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September 2021

Lactate and pyruvate levels correlation with lactate dehydrogenase gene expression and glucose consumption in Tamoxifen resistant MCF-7 cells using capillary electrophoresis with contactless conductivity detection (CE-C D).

Electrophoresis 2021 Oct 23. Epub 2021 Oct 23.

Department of Analytical Chemistry, Faculty of Pharmacy, Helwan University, Cairo, Egypt.

Breast cancer is the second leading cause of cancer death in women after lung cancer. The first line treatment of metastatic breast cancer in premenopausal women relies on tamoxifen. The development of tamoxifen resistance is not fully understood. Read More

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October 2021

A Facile Probe for Fluorescence Turn-on and Simultaneous Naked-Eyes Discrimination of HS and biothiols (Cys and GSH) and Its Application.

J Fluoresc 2021 Oct 23. Epub 2021 Oct 23.

Department of Chemistry, College of Art and Sciences, Northeast Agricultural University, Harbin, 150030, PR China.

Hydrogen sulfide and biothiol molecules such as Cys and GSH acted important roles in many physiological processes. To simultaneously detect and distinguish them was quite necessary by a suitable fluorescent probe. A novel chemosensor 4-(4-(benzo[d]thiazol-2-yl)-2-methoxyphenoxy)-7-nitrobenzo[c][1,2,5]oxadiazole (BMNO) was designed to detect HS/Cys/GSH using the combination of nitrobenzofurazan (NBD) and benzothiazole fluorophores linked by a facile ether bond. Read More

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October 2021

Computed-tomography angiography in arterial acute intestinal ischemia: prognostic interest of vascular semiology.

Abdom Radiol (NY) 2021 Oct 23. Epub 2021 Oct 23.

CHRU Brest Cavale Blanche, Bretagne, France.

Purpose: To investigate vascular features on abdominal Computed-Tomography Angiography (CTA) correlated with 48-h mortality in patients who underwent arterial acute intestinal ischemia (AAII) surgery. The secondary objective was to create a prognostic score on the 48-h mortality after surgery, based on the most relevant signs.

Method: We included 104 patients who underwent surgery for acute mesenteric ischemia. Read More

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October 2021

REPRISAL: Mapping lignification dynamics using chemistry, data segmentation, and ratiometric analysis.

Plant Physiol 2021 Oct 23. Epub 2021 Oct 23.

Univ. Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France.

This article describes a methodology for detailed mapping of the lignification capacity of plant cell walls that we have called "REPRISAL" for REPorter Ratiometrics Integrating Segmentation for Analyzing Lignification. REPRISAL consists of the combination of three separate approaches. In the first approach, H*, G* and S* monolignol chemical reporters, corresponding to p-coumaryl alcohol, coniferyl alcohol and sinapyl alcohol, are used to label the growing lignin polymer in a fluorescent triple labelling strategy based on the sequential use of 3 main bioorthogonal chemical reactions. Read More

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October 2021

The natural history of emerging diabetic retinopathy and microalbuminuria from prepuberty to early adulthood in Type 1 diabetes: A 19-year prospective clinical follow-up study.

Diabet Med 2021 Oct 23:e14732. Epub 2021 Oct 23.

Department of Pediatrics, PEDEGO Research Group and Medical Research Center, University of Oulu, Oulu University Hospital, PO Box 23, 90029 OYS, Oulu, Finland.

Objective: To evaluate the impact of long-term glycemic control and glycemic variability on microvascular complications in adolescents and young adults with childhood-onset Type 1 diabetes.

Methods: Twenty-six participants took part in a prospective follow-up study. We used univariate generalised estimating equations (GEE) analysis with first-order autoregressive AR(1) covariance structure for repeated measurements to evaluate relationship between emerging diabetic retinopathy (DR) and each single explanatory variable, namely age at developmental stages from late prepuberty until early adulthood, duration of diabetes and long-term HbA1c. Read More

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October 2021

High D-index during mobilization predicts poor mobilization of CD34+ cells after anti-lymphoma salvage chemotherapy.

J Clin Apher 2021 Oct 23. Epub 2021 Oct 23.

Department of Hematology & Oncology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.

Background: Performing stem cell collection after mobilization chemotherapy was a well-balanced strategy between anti-tumor effect and efficient collection of CD34+ cells, but deep and prolonged nadir exposed patients to risk of febrile neutropenia. Febrile neutropenia was known to be associated with lower yields of CD34+ cells, but quantitative data referring to association between yields of CD34+ cells and severity of neutropenia was lacking. We hypothesized that D-index, which was developed for quantitative evaluation of severity of neutropenia especially in the field of hematologic malignancies, could predict yields of CD34+ cells. Read More

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October 2021