10,363 results match your criteria aspergillus niger

Biochar as a biocompatible mild anti-inflammatory supplement for animal feed and agricultural fields.

Chem Biodivers 2021 Apr 9. Epub 2021 Apr 9.

Mersin Universitesi, Biotechnology, Çiftlikköy Kampüsü Fen Edeb. Fakültesi Biyoteknoloji Bölümü, 33310, Mersin, TURKEY.

Biochar is an organic material and high in carbon content, besides its use for energy purposes, it is also a material that serves the purpose of improving soil fertility, organic matter content of soils and removing heavy metals from water and soil. This study aims to investigate the antimicrobial effects of biochar whose beneficial effects on agricultural productivity has been proven by different studies. Scientific literature concerning the antibacterial, antifungal and antiviral effects of the apricot seed and olive seed biochar is limited. Read More

View Article and Full-Text PDF

Regioselective chemoenzymatic syntheses of ferulate conjugates as chromogenic substrates for feruloyl esterases.

Beilstein J Org Chem 2021 1;17:325-333. Epub 2021 Feb 1.

Toulouse Biotechnology Institute, Bio & Chemical Engineering (TBI), Université de Toulouse, CNRS 5504, INRAE 792, INSA de Toulouse, 135 Avenue de Rangueil, 31077 Toulouse, France.

Generally, carbohydrate-active enzymes are studied using chromogenic substrates that provide quick and easy color-based detection of enzyme-mediated hydrolysis. For feruloyl esterases, commercially available chromogenic ferulate derivatives are both costly and limited in terms of their experimental application. In this study, we describe solutions for these two issues, using a chemoenzymatic approach to synthesize different ferulate compounds. Read More

View Article and Full-Text PDF
February 2021

An overview of Trichoderma reesei co-cultures for the production of lignocellulolytic enzymes.

Appl Microbiol Biotechnol 2021 Apr 7. Epub 2021 Apr 7.

Laboratory of Enzymology, Department of Cellular Biology, University of Brasília, Brasília, Brazil.

Biorefineries are core facilities for implementing a sustainable circular bioeconomy. These facilities rely on microbial enzymes to hydrolyze lignocellulosic substrates into fermentable sugars. Fungal co-cultures mimic the process of natural biodegradation and have been shown to increase certain enzyme activities. Read More

View Article and Full-Text PDF


Antimicrob Agents Chemother 2021 Apr 5. Epub 2021 Apr 5.

Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, DK-9220, Aalborg East, Denmark

is an opportunistic pathogen commonly found in a variety of indoor and outdoor environments. An environmental isolate of from a pig farm were resistant to itraconazole and in-depth investigations were conducted to better understand cellular responses during growth when exposed to an antifungal.Using a combination of cultivation techniques, antibiotic stress-testing, and label-free proteomics, this study has investigated the physiological and metabolic responses of to sublethal levels of antifungal stress. Read More

View Article and Full-Text PDF

Identification of Dairy Fungal Contamination and Reduction of Aflatoxin M1 Amount by Three Acid and Bile Resistant Probiotic Bacteria.

Arch Razi Inst 2021 Mar 1;76(1):119-126. Epub 2021 Mar 1.

Department of Food Microbiology, Faculty of Veterinary Specialized Science, Science and Research Branch, Tehran Central Branch, Islamic Azad University, Tehran, Iran.

Aflatoxins (AFs) released by fungi are observed in the cow’s milk even after pasteurization. Aflatoxin M1 (AFM1) has particularly an incredible clinical significance, as a critical carcinogenic agent for humans. Several strategies have been implemented for lowering the AFM1 amount, such as the employment of probiotics, particularly lactobacilli or lactic acid bacteria (LAB). Read More

View Article and Full-Text PDF

Microwave-assisted solvent-free extraction of essential oil from anti-phytopathogenic potential for fruit post-harvesting.

3 Biotech 2021 Apr 15;11(4):166. Epub 2021 Mar 15.

Institute of Sustainable Agrotechnology, Universiti Malaysia Perlis, 02100 Padang Besar, Perlis Malaysia.

This work evaluates the fungicidal effect of essential oil from () by solvent-free microwave-assisted extraction with a yield of 0.54%. Fourier-transform infrared spectroscopy was utilised to identify the functional groups, which were O-H, C-O, C-H, and C=C. Read More

View Article and Full-Text PDF

Design and Synthesis, Biological Evaluation of Bis-(1,2,3- and 1,2,4)-Triazole Derivatives as Potential Antimicrobial and Antifungal Agents.

Bioorg Med Chem Lett 2021 Mar 31:128004. Epub 2021 Mar 31.

Department of Chemistry, Nizam College, Osmania University, Hyderabad, Telangana, 500001, India. Electronic address:

A new series of bis-1,2,3- and 1,2,4-triazoles (10a-m) were designed and efficiently synthesized using methyl salicylate as a starting material as potential antimicrobial agents. All compounds were characterized by their proton &C NMR, IR, Mass spectral data, and elemental analysis. The final compounds 10a-m were in vitro screened for antimicrobial and antifungal activity against gram negative Pseudomonas aeruginosa, Escherichia coli, gram positive Bacillus subtilis, Staphylococcus aureus strains and Aspergillus niger &Saccharomyces cerevisiae. Read More

View Article and Full-Text PDF

Repeated exposure of spores to the antifungal bacterium Ter331 selects for delayed spore germination.

Appl Environ Microbiol 2021 Apr 2. Epub 2021 Apr 2.

Department of Plant Pathology, University of California Davis, Davis, California, USA.

The bacterial strain Ter331 (CfTer331) inhibits mycelial growth and spore germination in N402 (AnN402). The mechanisms underlying this antagonistic bacterial-fungal interaction have been extensively studied, but knowledge on the long-term outcome of this interaction is currently lacking. Here, we used experimental evolution to explore the dynamics of fungal adaptation to recurrent exposure to CfTer331. Read More

View Article and Full-Text PDF

Microbial Application to Improve Olive Mill Wastewater Phenolic Extracts.

Molecules 2021 Mar 30;26(7). Epub 2021 Mar 30.

Council for Agricultural Research and Economics (CREA), Research Centre for Olive, Fruit and Citrus Crops, 95024 Acireale, Italy.

Olive mill wastewater (OMW) contains valuable and interesting bioactive compounds, among which is hydroxytyrosol, which is characterized by a remarkable antioxidant activity. Due to the health claims related to olive polyphenols, the aim of this study was to obtain an extract from OMW with an increased level of hydroxytyrosol by means of microbial enzymatic activity. For this purpose, four commercial adsorbent resins were selected and tested. Read More

View Article and Full-Text PDF

Stimulatory Effects of Oleci Acid and Fungal Elicitor on Betulinic Acid Production by Submerged Cultivation of Medicinal Mushroom .

J Fungi (Basel) 2021 Mar 31;7(4). Epub 2021 Mar 31.

Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China.

To evaluate the novel strategy of oleic acid and fungal elicitor (made from ) to elicit betulinic acid biosynthesis in medicinal mushroom , we conduct the stimulatory effects investigation for synthesizing betulinic acid from betulin. HPLC results indicated oleic acid and fungal elicitor were effective stimulators. The supplementation of 1. Read More

View Article and Full-Text PDF

Technological and Safety Attributes of Lactic Acid Bacteria and Yeasts Isolated from Spontaneously Fermented Greek Wheat Sourdoughs.

Microorganisms 2021 Mar 24;9(4). Epub 2021 Mar 24.

Laboratory of Food Quality Control and Hygiene, Department of Food Science and Human Nutrition, Agricultural University of Athens, 11855 Athens, Greece.

The aim of the present study was to assess the technological and safety potential of 207 lactic acid bacteria (LAB) and 195 yeast strains isolated from spontaneously fermented Greek wheat sourdoughs. More accurately, the amylolytic, proteolytic, lipolytic, phytase and amino acid decarboxylase activities, along with the production of exopolysaccharides and antimicrobial compounds by the LAB and yeast isolates, were assessed. A well diffusion assay revealed seven proteolytic LAB and eight yeast strains; hydrolysis of tributyrin was evident only in 11 LAB strains. Read More

View Article and Full-Text PDF

New Insights into Antibacterial and Antifungal Properties, Cytotoxicity and Aquatic Ecotoxicity of Flame Retardant PA6/DOPO-Derivative Nanocomposite Textile Fibers.

Polymers (Basel) 2021 Mar 15;13(6). Epub 2021 Mar 15.

National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia.

The aim of this study was to evaluate the antibacterial and antifungal activity, cytotoxicity, leaching, and ecotoxicity of novel flame retardant polyamide 6 (PA6) textile fibers developed by our research group. The textile fibers were produced by the incorporation of flame-retardant bridged 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide (DOPO) derivative (PHED) in the PA6 matrix during the in situ polymerization process at concentrations equal to 10 and 15 wt% (PA6/10PHED and PA6/15PHED, respectively). Whilst the nanodispersed PHED provided highly efficient flame retardancy, its biological activity led to excellent antibacterial activity against and , as well as excellent antifungal activity against and . Read More

View Article and Full-Text PDF

Enhanced Antimould Action of Surface Modified Copper Oxide Nanoparticles with Phenylboronic Acid Surface Functionality.

Biomimetics (Basel) 2021 Mar 15;6(1). Epub 2021 Mar 15.

Department of Chemistry and Biochemistry, University of Hull, Hull HU67RX, UK.

Antimould agents are widely used in different applications, such as specialty paints, building materials, wood preservation and crop protection. However, many antimould agents can be toxic to the environment. This work aims to evaluate the application of copper oxide nanoparticles (CuONPs) surface modified with boronic acid (BA) terminal groups as antimould agents. Read More

View Article and Full-Text PDF

Efficacy Assessment of Biosynthesized Copper Oxide Nanoparticles (CuO-NPs) on Stored Grain Insects and Their Impacts on Morphological and Physiological Traits of Wheat ( L.) Plant.

Biology (Basel) 2021 Mar 17;10(3). Epub 2021 Mar 17.

Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, Egypt.

Herein, CuO-NPs were fabricated by harnessing metabolites of strain (G3-1) and characterized using UV-vis spectroscopy, XRD, TEM, SEM-EDX, FT-IR, and XPS. Spherical, crystallographic CuO-NPs were synthesized in sizes ranging from 14.0 to 47. Read More

View Article and Full-Text PDF

Comparative Study on the Essential Oils from Five Wild Egyptian Species: Effective Extraction Techniques, Antimicrobial Activity and In-Silico Analyses.

Antibiotics (Basel) 2021 Mar 3;10(3). Epub 2021 Mar 3.

Chemistry of Medicinal Plants Department, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, Egypt.

The genus is recognized in folk medicine for anti-inflammatory, anti-itch, antitussive, purgative, astringent, and tonic activities. To study the chemical determinant for antimicrobial activity essential oils (EOs), five species were analyzed including: , , , and . Conventional hydro-distillation (HD) and microwave-assisted extraction (MAE), as new green technologies, were compared for the extraction of essential oils. Read More

View Article and Full-Text PDF

New Approach for Using of L. and L. Essential Oils as Wood-Biofungicides: GC-MS, SEM, and MNDO Quantum Chemical Studies.

Materials (Basel) 2021 Mar 11;14(6). Epub 2021 Mar 11.

Botany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.

Background: Fungi growing on wood cause deterioration of stored food materials or discoloration of the wood itself, and the search for new and safe bioagents is recently needed.

Methods: Essential oils (EOs) from aerial parts from L. and L. Read More

View Article and Full-Text PDF

Antagonistic Activity of Bacteria Isolated from the L. Gut against Some Multidrug-Resistant Human Pathogens.

Antibiotics (Basel) 2021 Mar 11;10(3). Epub 2021 Mar 11.

Department of Entomology, Faculty of Science, Ain Shams University, P.O. Box 11566 Cairo, Egypt.

The insect gut is home to a wide range of microorganisms, including several bacterial species. Such bacterial symbionts provide various benefits to their insect hosts. One of such services is providing metabolites that resist infections. Read More

View Article and Full-Text PDF

Preservation stress resistance of melanin deficient conidia from Paecilomyces variotii and Penicillium roqueforti mutants generated via CRISPR/Cas9 genome editing.

Fungal Biol Biotechnol 2021 Apr 2;8(1). Epub 2021 Apr 2.

TIFN, Agro Business Park 82, 6708 PW, Wageningen, The Netherlands.

Background: The filamentous fungi Paecilomyces variotii and Penicillium roqueforti are prevalent food spoilers and are of interest as potential future cell factories. A functional CRISPR/Cas9 genome editing system would be beneficial for biotechnological advances as well as future (genetic) research in P. variotii and P. Read More

View Article and Full-Text PDF

N-1, 3-Benzenedicarbonyl-Bis-(Amino Acid) and Dipeptide Candidates: Synthesis, Cytotoxic, Antimicrobial and Molecular Docking Investigation.

Drug Des Devel Ther 2021 25;15:1315-1332. Epub 2021 Mar 25.

Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia.

Purpose: The objective of our work was to prepare a potent and safe antimicrobial and anticancer agents, through synthesis of several peptides and examine their biological activities, namely as, cytotoxically potent and antimicrobial and antifungal agents.

Introduction: Multidrug-resistant microbial strains have arisen against all antibiotics in clinical use. Infections caused by these bacteria threaten global public health and are associated with high mortality rates. Read More

View Article and Full-Text PDF

Successful use of Nicotiana tabacum hairy roots for the recombinant production of Cecropin A peptide.

Biotechnol Appl Biochem 2021 Mar 31. Epub 2021 Mar 31.

Institute of Biotechnology, Shiraz University, Shiraz, Iran.

Cecropin A, as an antimicrobial peptide (AMP), is possible to use in medical and agricultural fields as a new and safe bio-control agent. Therefore, it is highly necessary to find a cost-effective and scalable approach to generate a large scale of it. In this research, the Agrobacterium rhizogenes strain ATCC 15834 was used to transfer the Cecropin A gene to the Nicotiana tabacum. Read More

View Article and Full-Text PDF

Characterization and optimization of extracellular enzymes production by Aspergillus niger strains isolated from date by-products.

J Genet Eng Biotechnol 2021 Mar 31;19(1):50. Epub 2021 Mar 31.

Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed Premier University, 60 000, Oujda, Morocco.

Background: This work aims to study the optimal conditions of the fermentation culture medium used for the production of extracellular enzymes (amylase, cellulase, lipase, and protease) from previously isolated Aspergillus niger strains in date by-products.

Results: The five most powerful isolates selected based on the zone of degradation formed on Petri plates by the substrate were subjected to the quantitative evaluation of their enzymatic production. All five strains showed almost similar API-ZYM profiles, with minor variations observed at the level of some specific enzyme expression. Read More

View Article and Full-Text PDF

[CRISPR/Cas-based genome editing in Aspergillus niger].

Sheng Wu Gong Cheng Xue Bao 2021 Mar;37(3):980-990

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.

Aspergillus niger is a vital industrial workhouse widely used for the production of organic acids and industrial enzymes. This fungus is a crucial cell factory due to its innate tolerance to a diverse range of abiotic conditions, high production titres, robust growth during industrial scale fermentation, and status as a generally recognized as safe (GRAS) organism. Rapid development of synthetic biology and systems biology not only offer powerful approaches to unveil the molecular mechanisms of A. Read More

View Article and Full-Text PDF

Quality control, HPTLC analysis, antioxidant and antimicrobial activity of hydroalcoholic extract of roots of ( C.B Clarke).

Drug Metab Pers Ther 2020 Dec 11. Epub 2020 Dec 11.

Department of Biotechnology, Faculty of Natural Sciences, Jamia Millia Islamia, New Delhi, India.

Objectives: , CB Clarke () is a perennial herb of the Compositae family. The root of has been used for the treatment of various diseases such as hepatitis, jaundice, intestinal worms, bronchial asthma, and a variety of skin diseases. The aim of the study was to ensure quality control of and its preparation HAESL (hydroalcoholic extract of ) along with assessment of HAESL antimicrobial and antioxidant activities . Read More

View Article and Full-Text PDF
December 2020

CreA-mediated repression of gene expression occurs at low monosaccharide levels during fungal plant biomass conversion in a time and substrate dependent manner.

Cell Surf 2021 Dec 3;7:100050. Epub 2021 Mar 3.

Fungal Physiology, Westerdijk Fungal Biodiversity Institute, & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.

Carbon catabolite repression enables fungi to utilize the most favourable carbon source in the environment, and is mediated by a key regulator, CreA, in most fungi. CreA-mediated regulation has mainly been studied at high monosaccharide concentrations, an uncommon situation in most natural biotopes. In nature, many fungi rely on plant biomass as their major carbon source by producing enzymes to degrade plant cell wall polysaccharides into metabolizable sugars. Read More

View Article and Full-Text PDF
December 2021

Gene Expression, Biochemical Characterization of a sn-1, 3 Extracellular Lipase From GZUF36 and Its Model-Structure Analysis.

Front Microbiol 2021 12;12:633489. Epub 2021 Mar 12.

Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guizhou University, Guiyang, China.

In this study, a sn-1, 3 extracellular lipases from GZUF36 (PEXANL1) was expressed in , characterized, and the predicted structural model was analyzed. The optimized culture conditions of showed that the highest lipase activity of 66.5 ± 1. Read More

View Article and Full-Text PDF

Spray-induced gene silencing for disease control is dependent on the efficiency of pathogen RNA uptake.

Plant Biotechnol J 2021 Mar 27. Epub 2021 Mar 27.

Department of Microbiology & Plant Pathology, Center for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, California, 92521, USA.

Recent discoveries show that fungi can take up environmental RNA, which can then silence fungal genes through environmental RNA interference. This discovery prompted the development of Spray-Induced Gene Silencing (SIGS) for plant disease management. In this study, we aimed to determine the efficacy of SIGS across a variety of eukaryotic microbes. Read More

View Article and Full-Text PDF

Ochratoxin A and related fungi in Brazilian black pepper (Piper nigrum L.).

Food Res Int 2021 Apr 10;142:110207. Epub 2021 Feb 10.

Food Technology Institute, Microbiology Laboratory, Campinas, São Paulo, Brazil. Electronic address:

Ochratoxin A (OTA) is a mycotoxin with nephrotoxic, genotoxic, teratogenic and carcinogenic properties, produced by several species of Aspergillus, mainly those belonging to the A. section Circumdati and A. section Nigri. Read More

View Article and Full-Text PDF

Essential Oils of Polyalthia suberosa Leaf and Twig and Their cytotoxic and Anti-microbial Activities.

Chem Biodivers 2021 Mar 27. Epub 2021 Mar 27.

Vietnam Academy of Science and Technology, Institute of Natural Products Chemistry, Hoang Quoc Viet, Hanoi, VIET NAM.

Essential oils from the leaf and twig of Polyalthia suberosa (Roxb.) Thwaites were analyzed using GC-MS/FID. A total of sixty-three constituents were namely identified accounting for 96. Read More

View Article and Full-Text PDF

Rhizoremediation of hydrocarbon contaminated soil using (Mill) and associated fungi.

Int J Phytoremediation 2021 Mar 25:1-13. Epub 2021 Mar 25.

Department of Cell Biology and Genetics, University of Lagos, Lagos, Nigeria.

The potentials of and its rhizospheric, non-mycorrhizal fungi in biodegrading and bio-remediating hydrocarbon contaminated soil were investigated and Biodegradation study was done in two stages: preliminary study using hydrocarbon treated filter paper and with Mineral Salt Media (MSM) read on Spectrophotometer at two photo synthetically active wavelengths (530 nm and 620 nm) while rhizoremediation study was done in contaminated plot of land. Hydrocarbon utilization ability of the fungi and plant were confirmed using total petroleum hydrocarbon (TPH) analysis and gas chromatography mass spectroscopy (GC-MS). Results show differing rates of hydrocarbon utilization by isolated fungi. Read More

View Article and Full-Text PDF

Re-routing of Sugar Catabolism Provides a Better Insight Into Fungal Flexibility in Using Plant Biomass-Derived Monomers as Substrates.

Front Bioeng Biotechnol 2021 8;9:644216. Epub 2021 Mar 8.

Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Utrecht, Netherlands.

The filamentous ascomycete has received increasing interest as a cell factory, being able to efficiently degrade plant cell wall polysaccharides as well as having an extensive metabolism to convert the released monosaccharides into value added compounds. The pentoses D-xylose and L-arabinose are the most abundant monosaccharides in plant biomass after the hexose D-glucose, being major constituents of xylan, pectin and xyloglucan. In this study, the influence of selected pentose catabolic pathway (PCP) deletion strains on growth on plant biomass and re-routing of sugar catabolism was addressed to gain a better understanding of the flexibility of this fungus in using plant biomass-derived monomers. Read More

View Article and Full-Text PDF