New Phytol 2002 Mar 5;153(3):527-533. Epub 2002 Mar 5.
University of California, San Diego, Division of Biology, Cell and Developmental Biology Section and Center for Molecular Genetics, La Jolla, CA 92093-0116, USA; 1Present address: Department of Applied Biological Science, Tokyo University of Science, Noda, Chiba, 278-8510 Japan; 2Present address: Department of Plant Biology, University of Minnesota, St. Paul, MN 55108-1095, USA.
• Cytosolic calcium and pH changes are integral components of guard cell signal transduction. Acetoxymethyl (AM) ester-linked Ca sensitive dyes are usually degraded before loading by extracellular esterases or partitioned into plant vacuoles using standard techniques, thereby preventing cytoplasmic Ca imaging. • Here a method is described for improved loading of the calcium sensitive fluorescent dyes Calcium Green-1 and Fura-2 AM ester into the cytoplasm of Commelina and Arabidopsis guard cells in epidermal strips, allowing fluorescence from several guard cells in an epidermal strip to be imaged and measured simultaneously. Read More