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Development of duplex TaqMan-based real-time PCR assay for the simultaneous detection of Perkinsus olseni and P. chesapeaki in host Manila clam tissue samples.

J Invertebr Pathol 2021 May 7:107603. Epub 2021 May 7.

Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280, Plouzané, France. Electronic address:

The aetiological agent Perkinsus olseni is globally recognised as a major threat for shellfish production considering its wide geographical distribution across Asia, Europe, Australia and South America. Another species, Perkinsus chesapeaki, which has never been known to be associated with significant mortality events, was recently detected along French coasts infecting clam populations sporadically in association with P. olseni. Read More

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Effectiveness of ozone therapy in addition to conventional treatment on mortality in patients with COVID-19.

Int J Clin Pract 2021 May 10:e14321. Epub 2021 May 10.

University of Health Sciences, Haydarpasa Numune Training and Research Hospital, Department of Infectious Disease and Clinical Microbiology, Istanbul, Turkey.

Aim: In this study, we aimed to investigate the effectiveness of ozone therapy, which is one of the integrative medicine applications that has been used safely for many years, on the prevalence of mortality in patients receiving COVID-19 treatment.

Methods: This was a prospective, controlled study conducted on patients with COVID-19 who were hospitalized. In this study, 55 patients were included. Read More

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Microfabricated Device to Record Axonal Conduction under Pharmacological Treatment for Functional Evaluation of Axon Ion Channel.

IEEE Trans Biomed Eng 2021 May 10;PP. Epub 2021 May 10.

Objective: Neuronal networks are fundamental structures for information processing in the central nervous system. This processing function is severely impaired by abnormal axonal conduction from changes in functional ion channel expression. The evaluation of axonal conduction properties can be effective in the early diagnosis of information-processing abnormalities. Read More

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Pharmacokinetics Parameters of Diagoxin among Saudi Patients in Qassim Region, Saudi Arabia.

Drug Metab Lett 2021 May 6. Epub 2021 May 6.

Department of Respiratory Medicine, Our Lady of Lourdes Hospital, Drogheda, Ireland.

Background: The pharmacodynamic effects of digoxin are susceptible to multiple factors, most notably, heart uptake of the digoxin dose and its concentration in the serum. Another important factor to mention is the renal function state of an individual.

Objective: In this study, we aimed to develop a simple algorithm based on subsets of clinically relevant information, which will help to personalize digoxin based on pharmacokinetic (PK) approach, which can help on marketing the appropriate utilization of this medication. Read More

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Standardizing salivary lactoferrin measurements to obtain a robust diagnostic biomarker for Alzheimer's disease.

Alzheimers Dement (Amst) 2021 2;13(1):e12173. Epub 2021 May 2.

Network Center for Biomedical Research in Neurodegenerative Diseases (CIBERNED) Cordoba Spain.

The search for new, robust, and reproducible biomarkers for Alzheimer's disease (AD) diagnosis is a challenge. We recently reported that salivary lactoferrin (Lf) could be presented as new biomarker candidate for AD, being both non-invasive and cost-effective, as well as having appropriate diagnostic performance for the clinical detection of AD subjects. Saliva is an attractive sample type for biomarker-based testing approaches for several other diseases; however, its composition may change under certain circumstances. Read More

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Maximum Entropy Modeling to Predict the Impact of Climate Change on Pine Wilt Disease in China.

Front Plant Sci 2021 23;12:652500. Epub 2021 Apr 23.

Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Province Key Laboratory of Soil and Water Conservation and Ecological Restoration, Nanjing Forestry University, Nanjing, China.

Pine wilt disease is a devastating forest disease caused by the pinewood nematode , which has been listed as the object of quarantine in China. Climate change influences species and may exacerbate the risk of forest diseases, such as the pine wilt disease. The maximum entropy (MaxEnt) model was used in this study to identify the current and potential distribution and habitat suitability of three pine species and in China. Read More

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Preparation of ROS active and photothermal responsive hydroxyapatite nanoplatforms for anticancer therapy.

Mater Sci Eng C Mater Biol Appl 2021 Jun 14;125:112098. Epub 2021 Apr 14.

Jinzhou Medical University, Jinzhou 121001, Liaoning, China. Electronic address:

Photothermal responsive nanoplatforms are attracting for photothermal therapy (PTT) of cancer. Herein, we propose a strategy to prepare IR-780 modified hydroxyapatite (HAP) nanorods as photothermic agents (HAP@IR-780). The results demonstrated that the obtained HAP@IR-780 was photothermal responsive under near-infrared laser irradiation the photothermal conversion efficiency was 69. Read More

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A simple analytical methodology for platinum nanoparticles control in complex clinical matrices via SP-ICP-MS.

Talanta 2021 Aug 30;231:122370. Epub 2021 Mar 30.

Department of Analytical Chemistry and Food Technology, Faculty of Environmental Sciences and Biochemistry, University of Castilla-La Mancha, Avenida Carlos III s/n, 45071, Toledo, Spain. Electronic address:

A simple method based on the use of inductively coupled plasma mass spectrometry in single particle mode (SP-ICP-MS) has been proposed, for the first time, for the study of platinum nanoparticles (PtNPs) in complex clinical matrices such as human urine and blood serum. Critical parameters for signal acquisition were optimized to achieve a correct and simultaneous sizing and counting (particle-based in particles L and mass-based in ng L) of 50 and 70 nm PtNPs. Different reagents, as tetramethylammonium hydroxide (TMAH) and/or Triton X-100, and concentrations have been tested to ensure an adequate stabilization and extraction of PtNPs. Read More

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Organic carbon bioavailability: Is it a good driver to choose the best biological nitrogen removal process?

Sci Total Environ 2021 Apr 29;786:147390. Epub 2021 Apr 29.

Western Paraná State University, 85819-110 Cascavel, PR, Brazil; Federal University of Fronteira Sul, 99700-000 Erechim, Brazil; Embrapa Suínos e Aves, 89715-899 Concórdia, SC, Brazil. Electronic address:

Organic carbon can affect the biological nitrogen removal process since the Anammox, heterotrophic and denitrifying bacteria have different affinities and feedback in relation to carbon/nitrogen ratio. Therefore, we reviewed the wastewater carbon concentration, its biodegradability and bioavailability to choose the appropriate nitrogen removal process between conventional (nitrification-denitrification) and Anammox-based process (i.e. Read More

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Persistence of SARS-CoV-2 virus and viral RNA in relation to surface type and contamination concentration.

Appl Environ Microbiol 2021 May 7. Epub 2021 May 7.

Public Health England, National Infection Service, Porton Down, Wiltshire, UK

The transmission of SARS-CoV-2 is likely to occur through a number of routes, including contact with contaminated surfaces. Many studies have used RT-PCR analysis to detect SARS-CoV-2 RNA on surfaces but seldom has viable virus been detected. This paper investigates the viability over time of SARS-CoV-2 dried onto a range of materials and compares viability of the virus to RNA copies recovered, and whether virus viability is concentration dependant. Read More

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Tacrolimus Immunosuppression Causes Drug-Induced Hypersensitivity Reactions in Liver-Transplant Patients: A 3-Case Report.

Transplant Proc 2021 May 4. Epub 2021 May 4.

2nd Propaedeutic Department of Internal Medicine, Gastroenterology Section, Aristotle University of Thessaloniki, Hippokration Hospital, Thessaloniki, Greece.

Background: Drug-induced hypersensitivity reactions attributed to the immunosuppressive agent tacrolimus after an organ transplant are rare in the literature. We present 3 cases of male adult patients grafted with a cadaveric liver who developed delayed hypersensitivity reactions to tacrolimus in the form of the prolonged-release capsules (Advagraf). Furthermore, the appropriate drug concentration solutions used for allergy testing are proposed. Read More

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Darcy-Forchheimer hybrid nanofluid flow over a stretching curved surface with heat and mass transfer.

PLoS One 2021 7;16(5):e0249434. Epub 2021 May 7.

Faculty of Science and Technology, Department of Mathematics and Computer Science, Program in Applied Statistics, Rajamangala University of Technology Thanyaburi, Thanyaburi, Pathumthani, Thailand.

The present article provides a detailed analysis of the Darcy Forchheimer flow of hybrid nanoliquid past an exponentially extending curved surface. In the porous space, the viscous fluid is expressed by Darcy-Forchheimer. The cylindrical shaped carbon nanotubes (SWCNTs and MWCNTs) and Fe3O4 (iron oxide) are used to synthesize hybrid nanofluid. Read More

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Effect of Vitamin D Supplementation on Improvement of Symptoms in Mild-to-Moderate Asthma Patients with Vitamin D Insufficiency and Deficiency.

Tanaffos 2020 Dec;19(4):322-329

Department of Internal Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Background: In the current study, we assessed the effect of vitamin D supplementation on improvement of symptoms in mild-to-moderate asthma patients with vitamin D insufficiency and deficiency.

Materials And Methods: This randomized, controlled clinical trial included 132 mild-to-moderate asthma patients with vitamin D insufficiency (n=66) and those with vitamin D deficiency (n=66). They were assigned randomly to two groups of cases (with two subgroups) and controls (with two subgroups). Read More

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December 2020

Large scale purification and characterization of A21 deamidated variant-most prominent post translational modification (PTM) for insulins which is also widely observed in insulins pharmaceutical manufacturing and storage.

Protein Expr Purif 2021 May 3;185:105895. Epub 2021 May 3.

Biocon Biologics Limited (BBL), Research and Development (RND), Biocon Park, Bommasandra Jigani Link Road, Bangalore, 560 099, India. Electronic address:

Biopharmaceutical development demands appropriate understanding of product related variants, which are formed due to post-translational modification and during downstream processing. These variants can lead to low yield, reduced biological activity, and suboptimal product quality. In addition, these variants may undergo immune reactions, henceforth need to be appropriately controlled to ensure consistent product quality and patient safety. Read More

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Minimum inhibitory concentration (MIC) of Ceftriaxone and Azithromycin for blood culture isolates of Salmonella enterica spp.

J Infect Dev Ctries 2021 Apr 30;15(4):538-543. Epub 2021 Apr 30.

Department of Pathology and Microbiology, Breach Candy Hospital Trust, Mumbai, India.

Introduction: Enteric fever caused by Salmonella enterica continues to be a major public health problem worldwide. In the last decade, ceftriaxone and azithromycin have become the drugs of choice for treating enteric fever caused by Nalidixic acid resistant Salmonella (NARS) enterica. This has led to reports of drug resistance to both drugs. Read More

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Enhancing between-facility reproducibility of the SH test as an in vitro skin sensitization test by the improved test method.

J Toxicol Sci 2021 ;46(5):235-248

Graduate School of Environment and Information Sciences, Yokohama National University.

There has been an increased demand to eliminate animal experiments and to replace the experiments with alternative tests for assessing the safety of cosmetics. The SH test is an in vitro skin sensitization test that evaluates the protein binding abilities of a test substance. Skin sensitization must be evaluated by multiple test methods. Read More

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January 2021

Determination of spatial mercury concentration by laser ablation-inductively coupled plasma mass spectrometry.

J Toxicol Sci 2021 ;46(5):193-198

Graduate School of Pharmaceutical Sciences, Chiba University.

Laser ablation-inductively coupled plasma mass spectrometry (LA-ICP-MS) is capable of metal imaging by acquiring local spatial information. However, the preparation of an appropriate standard for quantitative analysis is difficult because the matrices between the standard and the sample should match, and homogeneity of metal concentration in the standard is required. Hence, the aim of this study was to establish a highly quantitative mercury imaging method that utilizes LA-ICP-MS and an appropriate mercury standard consisting of rat tissue. Read More

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January 2021

Targeted Protein Quantification Using Parallel Reaction Monitoring (PRM).

Methods Mol Biol 2021 ;2228:145-157

Medizinisches Proteom-Center (MPC), Ruhr-Universität Bochum, Bochum, Germany.

Targeted proteomics represents an efficient method to quantify proteins of interest with high sensitivity and accuracy. Targeted approaches were first established for triple quadrupole instruments, but the emergence of hybrid instruments allowing for high-resolution and accurate-mass measurements of MS/MS fragment ions enabled the development of parallel reaction monitoring (PRM). In PRM analysis, specific peptides are measured as representatives of proteins in complex samples, with the full product ion spectra being acquired, allowing for identification and quantification of the peptides. Read More

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January 2021

Oral bioavailability and pharmacokinetics of esculetin following intravenous and oral administration in rats.

Xenobiotica 2021 May 5:1-24. Epub 2021 May 5.

College of Pharmacy, Kyungsung University, 309, Suyeong-ro, Nam-gu, Busan, 48434, Republic of Korea.

Esculetin is the main active ingredient isolated from (Nakai) Pamp. and L. The present study investigated the oral bioavailability and pharmacokinetics of esculetin in rats, following intravenous and oral administration. Read More

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Establishing local reference intervals for full blood count and white blood cell differential counts in Cape Town, South Africa.

S Afr Med J 2021 Mar 31;111(4):327-332. Epub 2021 Mar 31.

Division of Haematology, Department of Pathology, Faculty of Health Sciences, University of Cape Town, South Africa; National Health Laboratory Service, Groote Schuur Hospital, Cape Town, South Africa.

Background: Accurate laboratory reference intervals (RIs) are essential to differentiate between health and disease. There are variations in haematological indices within populations relating to gender, age, ethnicity and environment. Iron deficiency is common, has a wide range of clinical morbidities and affects red cell indices. Read More

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Evaluation of the Roche MagNA Pure 96 nucleic acid extraction platform for the Seegene Anyplex II HPV28 detection assay.

J Appl Microbiol 2021 May 3. Epub 2021 May 3.

Centre for Women's Infectious Diseases, The Royal Women's Hospital, Melbourne, Victoria, Australia.

Aim: Validate the Roche, MagNAPure96 (MP96) nucleic acid extraction platform for Seegene Anyplex II HPV28 (Anyplex28) detection of Human Papillomavirus.

Methods And Results: Comparisons were made for Anyplex28 genotyping from 115 cervical samples extracted on the Hamilton, STARlet and the MP96. Two DNA concentrations were used for the MP96, one matched for sample input to the STARlet, and another 5x concentration (laboratory standard). Read More

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Pharmacokinetic comparison of nalbuphine with single injection and patient-controlled analgesia mimic method in healthy Chinese volunteers.

J Clin Pharm Ther 2021 May 3. Epub 2021 May 3.

Department of Clinical Research Center, Central Hospital Affiliated to Shandong First Medical University, Jinan, China.

What Is Known And Objective: Nalbuphine is a mu (μ) receptor partial antagonist/kappa (κ) receptor agonist analgesic and can be administered as a single injection or using patient-controlled analgesia (PCA) in the clinical setting. However, differences in the pharmacokinetics of the two administration methods are unclear. Here, a clinical trial was performed to compare the pharmacokinetic characteristics and superiority of nalbuphine with a single-injection or PCA-mimic method to provide a reference for the selection of an appropriate administration method. Read More

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The relationship of different levels of high iodine and goiter in school children: a meta-analysis.

Nutr Metab (Lond) 2021 May 3;18(1):46. Epub 2021 May 3.

Department of Nutrition and Food Hygiene, School of Public Health, Tianjin Medical University, Heping District, No. 22, Qixiangtai Road, Tianjin, China.

Background: Over the past decade, the phenomenon of high urine iodine (HUI) and high water iodine (HWI) has become more common. But the risk of goiter caused by different levels of HUI and HWI remains unclear.

Objectives: To explore the risk of goiter development caused by HUI and HWI, and compare the risk of goiter development from different levels of high iodine. Read More

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Measuring transfer of human norovirus during sandwich production: Simulating the role of food, food handlers and the environment.

Int J Food Microbiol 2021 Mar 11;348:109151. Epub 2021 Mar 11.

Institute of Infection and Global Health, University of Liverpool, Liverpool, UK; NIHR Health Protection Research Unit in Gastrointestinal Infections, UK.

Foodborne outbreaks associated with transmission of norovirus are increasingly becoming a public health concern. Foods can be contaminated with faecal material at the point of production or during food preparation, in both the home and in commercial premises. Transmission of norovirus occurs through the faecal-oral route, either via person-to-person contact or through faecal-contamination of food, water, or environmental surfaces. Read More

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Improved extraction of acid-insoluble monosulfide minerals by stannous chloride reduction and its application to the separation of mono- and disulfide minerals in the presence of ferric iron.

Sci Total Environ 2021 Apr 27;785:147367. Epub 2021 Apr 27.

School of Environment and Energy, South China University of Technology, Guangzhou 510006, China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, South China University of Technology, Guangzhou 510006, China; Guangdong Provincial Key Laboratory of Solid Wastes Pollution Control and Recycling, South China University of Technology, Guangzhou 510006, China. Electronic address:

Metal sulfides, which are important indicators of sulfur cycling, are usually divided into two categories according to sulfur chemical valence: (1) monosulfides (S) and (2) disulfides (S). The two sulfur species are separated and quantified by a sequential-extraction method. Specifically, monosulfides are extracted as acid-volatile sulfide (AVS) using 6 M HCl prior to the extraction of disulfides using acidic CrCl, which is defined as chromium-reducible sulfur (CRS). Read More

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Investigation of intra-day variability of gaseous measurements in sheep using Portable accumulation chambers 1.

J Anim Sci 2021 May 3. Epub 2021 May 3.

Teagasc, Animal and Grassland Research and Innovation Centre, Athenry, Co. Galway, Ireland.

Portable accumulation chambers (PAC) enable short term spot measurements of gaseous emissions including methane (CH4), carbon dioxide (CO2) and oxygen (O2) consumption from small ruminants. To date the differences in morning and evening gaseous measurements in the PAC have not been investigated. The objectives of this study were to investigate: 1) the optimal measurement time in the PAC, 2) the appropriate method of accounting for the animal's size when calculating the animal's gaseous output, and 3) the intra-day variability of gaseous measurements. Read More

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Long-term effects of liquid swine manure land surface application in an apple orchard field on soil bacterial community and heavy metal contents in apple (Malus pumila Mill.).

Environ Sci Pollut Res Int 2021 May 3. Epub 2021 May 3.

Department of Animal Science and Technology, Shandong Agricultural University, Taian, 271018, People's Republic of China.

This study investigated the impact of liquid swine manure (LSM) land surface application in an apple orchard on soil health and copper (Cu) and zinc (Zn) in soil and apple. Three apple plots were selected, among which two for LSM application for 5 (AY5) and 11 (AY11) years with different application rates, a long-term inorganic fertilizer application plot as the control treatment (AY0). The soil and apple samples were collected for analysis of soil physicochemical properties, bacterial diversity and abundance, and the contents of Cu and Zn in soil and apple. Read More

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The Impact of Acute and Chronic Exercise on Immunoglobulins and Cytokines in Elderly: Insights From a Critical Review of the Literature.

Front Immunol 2021 14;12:631873. Epub 2021 Apr 14.

Biomedical Research Center, Qatar University, Doha, Qatar.

The level of immunoglobulins and cytokines changes with an ageing immune system. This review summarizes findings from studies that have examined the impact of acute and chronic exercise on immunoglobulins and cytokines in the elderly. Our literature analysis revealed that acute endurance exercise resulted in increased secretory salivary immunoglobulin A (SIgA), while acute bouts of muscle strengthening exercise (i. Read More

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Dehydrocostus Lactone Attenuates the Senescence of Nucleus Pulposus Cells and Ameliorates Intervertebral Disc Degeneration via Inhibition of STING-TBK1/NF-κB and MAPK Signaling.

Front Pharmacol 2021 14;12:641098. Epub 2021 Apr 14.

Shanghai Key Laboratory of Orthopaedic Implant, Shanghai Ninth People's Hospital, Shanghai, China.

The progression of intervertebral disc degeneration (IDD) is multifactorial with the senescence of nucleus pulposus (NP) cells and closely related to inflammation in NP cells. Dehydrocostus lactone (DHE) is a natural sesquiterpene lactone isolated from medicinal plants that has anti-inflammatory properties. Thus, DHE may have a therapeutic effect on the progression of IDD. Read More

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UV spectroscopic method for estimation of temozolomide: Application in stability studies in simulated plasma pH, degradation rate kinetics, formulation design, and selection of dissolution media.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Apr 17;258:119848. Epub 2021 Apr 17.

Industrial Research Laboratory, Department of Pharmacy, Birla Institute of Technology and Science, Pilani, Pilani Campus, India. Electronic address:

Temozolomide (TMZ) is a broad spectrum alkylating agent found effective in the treatment of glioblastoma multiforme, refractory anaplastic astrocytoma, and metastatic melanoma. The major drawback associated with TMZ is pH-dependent stability and short half-life. At physiological pH, it undergoes conversion to MTIC (methyltriazine imidazole carboxamide) and AIC (amino imidazole carboxamide), resulting in only 20-30% brain bioavailability. Read More

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