2,486 results match your criteria apoptotic patterns

Phosphoproteomics identifies PI3K inhibitor-selective adaptive responses in pancreatic cancer cell therapy and resistance.

Mol Cancer Ther 2021 Sep 22. Epub 2021 Sep 22.

U1037, Equipe Soutenue par la Région et par l'Inserm

The PI3K pathway is highly active in human cancers. The four class I isoforms of PI3K are activated by distinct mechanisms leading to a common downstream signaling. Their downstream redundancy is thought to be responsible for treatment failures of PI3K inhibitors. Read More

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September 2021

Bioinformatics and Immune Infiltration Analyses Reveal the Key Pathway and Immune Cells in the Pathogenesis of Hypertrophic Cardiomyopathy.

Front Cardiovasc Med 2021 23;8:696321. Epub 2021 Aug 23.

Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

This study was designed to identify the key pathway and immune cells for hypertrophic cardiomyopathy (HCM) via bioinformatics analyses of public datasets and evaluate the significance of immune infiltration in the pathogenesis of HCM. Expressional profiling from two public datasets (GSE36961 and GSE141910) of human HCM and healthy control cardiac tissues was obtained from the GEO database. After data preprocessing, differentially expressed genes (DEGs) were then screened between HCM and healthy control cardiac tissues in parallel. Read More

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Ferroptosis: A Trigger of Proinflammatory State Progression to Immunogenicity in Necroinflammatory Disease.

Front Immunol 2021 18;12:701163. Epub 2021 Aug 18.

Department of Emergency, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

Until recently, necrosis is generally regarded as traumatic cell death due to mechanical shear stress or other physicochemical factors, while apoptosis is commonly thought to be programmed cell death, which is silent to immunological response. Actually, multiple modalities of cell death are programmed to maintain systematic immunity. Programmed necrosis, such as necrosis, pyroptosis, and ferroptosis, are inherently more immunogenic than apoptosis. Read More

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TAZ is indispensable for c-MYC-induced hepatocarcinogenesis.

J Hepatol 2021 Aug 28. Epub 2021 Aug 28.

Department of Bioengineering and Therapeutic Sciences and Liver Center, University of California, San Francisco, California, USA. Electronic address:

Background & Aims: Mounting evidence implicates the Hippo downstream effectors Yes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ) in hepatocellular carcinoma (HCC). We investigated the functional contribution of YAP and/or TAZ during c-MYC induced liver tumor development.

Methods: YAP and/or TAZ requirement for c-Myc driven hepatocarcinogenesis was analyzed using conditional Yap, Taz, and Yap;Taz knockout (KO) mice. Read More

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Synthesis and Characterization of Zinc Oxide Nanoparticles of Solanum nigrum and Its Anticancer Activity via the Induction of Apoptosis in Cervical Cancer.

Biol Trace Elem Res 2021 Aug 27. Epub 2021 Aug 27.

Department of Biochemistry, Bharathiar University, Coimbatore, Tamil Nadu, India.

Effective cancer therapy can be achieved by using nano-drug delivery systems which provide a targeted drug delivery strategy by overcoming the drawbacks of conventional treatments like chemotherapy and radiation. ZnO nanoparticles are a potent anticancer agent that causes tumor cell destruction with the targeted drug delivery. In this present study, green synthesis of ZnO nanoparticles has been done using the plant Solanum nigrum. Read More

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Changes of Gene Expression Patterns from Aquatic Organisms Exposed to Metal Nanoparticles.

Int J Environ Res Public Health 2021 08 7;18(16). Epub 2021 Aug 7.

Institute of Biology, University of Szczecin, 71-415 Szczecin, Poland.

Metal nanoparticles are used in various branches of industry due to their physicochemical properties. However, with intensive use, most of the waste and by-products from industries and household items, and from weathering of products containing nanoparticles, end up in the waters. These pollutants pose a risk to aquatic organisms, one of which is a change in the expression of various genes. Read More

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Evolutionary profile for (host and viral) MLKL indicates its activities as a battlefront for extensive counteradaptation.

Mol Biol Evol 2021 Aug 26. Epub 2021 Aug 26.

Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America.

Pathogen infection triggers host innate defenses which may result in the activation of regulated cell death (RCD) pathways such as apoptosis. Given a vital role in immunity, apoptotic effectors are often counteracted by pathogen-encoded antagonists. Mounting evidence indicates that programmed necrosis, which is mediated by the RIPK3/MLKL axis and termed necroptosis, evolved as a countermeasure to pathogen-mediated inhibition of apoptosis. Read More

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Diverse transcriptional regulation and functional effects revealed by CRISPR/Cas9-directed epigenetic editing.

Oncotarget 2021 Aug 17;12(17):1651-1662. Epub 2021 Aug 17.

Division of Molecular Pathology, Oncode Institute, The Netherlands Cancer Institute, Amsterdam, The Netherlands.

DNA methylation is an epigenetic process that controls DNA accessibility and serves as a transcriptomic switch when deposited at regulatory regions. The adequate functioning of this process is indispensable for tissue homeostasis and cell fate determination. Conversely, altered DNA methylation patterns result in abnormal gene transcription profiles that contribute to tumor initiation and progression. Read More

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N-3-oxododecanoyl homoserine lactone exacerbates endothelial cell death by inducing receptor-interacting protein kinase 1-dependent apoptosis.

Am J Physiol Cell Physiol 2021 Oct 25;321(4):C644-C653. Epub 2021 Aug 25.

Department of Internal Medicine, Myongji Hospital, Hanyang University College of Medicine, Goyang, South Korea.

Endothelial dysfunction is associated with the initiation of sepsis-associated organ failure. Bacterial quorum-sensing molecules act as pathogen-associated molecular patterns; however, the effects of quorum-sensing molecules on endothelial cells remain less understood. This study investigated the molecular mechanisms of quorum-sensing molecule-induced cell death and their interaction with lipopolysaccharide (LPS) in human umbilical vein endothelial cells. Read More

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October 2021

Effects of demethylase KDM4B on the biological characteristics and function of yak cumulus cells in vitro.

Theriogenology 2021 Oct 17;174:85-93. Epub 2021 Aug 17.

Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Exploitation of Ministry of Education, Southwest Minzu University, Chengdu, Sichuan, 610041, PR China. Electronic address:

The present study aims to investigate the expression and function of lysine-specific demethylase 4B (KDM4B) in yak cumulus cells (CCs) in order to reveal the mechanisms by which KDM4B regulates biological characteristics and function of CCs. The cellular location of KDM4B and the methylation pattern of H3K9 were detected using immunofluorescence (IF) staining in CCs. The mRNA expression levels of apoptosis-related genes (BCL-2, HAX1 and BAX) and genes related to the estrogen pathway (ESR2, CYP17 and 3B-HSD) were estimated by qRT-PCR after knockdown of KDM4B expression by siRNA in yak CCs. Read More

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October 2021

Suppressed PLIN3 frequently occurs in prostate cancer, promoting docetaxel resistance via intensified autophagy, an event reversed by chloroquine.

Med Oncol 2021 Aug 19;38(10):116. Epub 2021 Aug 19.

Department of Radiotherapy/Oncology, Democritus University of Thrace, PO BOX 12, 68100, Alexandroupolis, Greece.

Lipid metabolism reprogramming is one of the adaptive events that drive tumor development and survival, and may account for resistance to chemotherapeutic drugs. Perilipins are structural proteins associated with lipophagy and lipid droplet integrity, and their overexpression is associated with tumor aggressiveness. Here, we sought to explore the role of lipid droplet-related protein perilipin-3 (PLIN3) in prostate cancer (PCa) chemotherapy. Read More

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Acute heat stress reduces viability but increases lactate secretion of porcine immature Sertoli cells through transcriptome reprogramming.

Theriogenology 2021 Oct 9;173:183-192. Epub 2021 Aug 9.

College of Animal Science, Yangtze University, Jingzhou, 434025, Hubei, China. Electronic address:

Sertoli cells, important constituents of the somatic niche, supports the growth and development of spermatogonia. Heat stress (HS), among multiple intrinsic and external factors, can induce physiological and biochemical changes in Sertoli cells. However, the underlying molecular mechanism remains largely unclear. Read More

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October 2021

Transcriptomic analysis of rat kidney reveals a potential mechanism of sex differences in susceptibility to cisplatin-induced nephrotoxicity.

Free Radic Biol Med 2021 10 9;174:100-109. Epub 2021 Aug 9.

Department of Biotechnology, The Catholic University of Korea, Bucheon, 14662, South Korea; Department of Medical and Biological Sciences, The Catholic University of Korea, Bucheon, 14662, South Korea. Electronic address:

Although cisplatin is an effective platinum-based anticancer drug against solid cancer, its availability is limited owing to its adverse side effects. Our study aimed to identify the potential relationship within cisplatin-induced multi-organ physiological changes and genetic factors associated with sex differences in nephrotoxicity susceptibility. To investigate this, mice received a single intraperitoneal injection of cisplatin. Read More

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October 2021

Patterns of Cell Death Induced by Thiohydantoins in Human MCF-7 Breast Cancer Cells.

Anticancer Agents Med Chem 2021 Aug 10. Epub 2021 Aug 10.

Laboratory of Immunoparasitology of Neglected Diseases and Cancer, State University of Londrina, PR. Brazil.

Background: Conventional therapies for breast cancer is still a challenge due to use of cytotoxic drugs not highly effective with major adverse effects. Thiohydantoins, are biologically active heterocyclic compounds reported by several biological activities, including anticarcinogenic properties, i.e. Read More

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Unfolded Protein Response Inhibition Reduces Middle East Respiratory Syndrome Coronavirus-Induced Acute Lung Injury.

mBio 2021 08 10;12(4):e0157221. Epub 2021 Aug 10.

Department of Epidemiology, University of North Carolina at Chapel Hillgrid.10698.36, Chapel Hill, North Carolina, USA.

Tissue- and cell-specific expression patterns are highly variable within and across individuals, leading to altered host responses after acute virus infection. Unraveling key tissue-specific response patterns provides novel opportunities for defining fundamental mechanisms of virus-host interaction in disease and the identification of critical tissue-specific networks for disease intervention in the lung. Currently, there are no approved therapeutics for Middle East respiratory syndrome coronavirus (MERS-CoV) patients, and little is understood about how lung cell types contribute to disease outcomes. Read More

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Anticancer effects of oleanolic acid on human melanoma cells.

Chem Biol Interact 2021 Sep 5;347:109619. Epub 2021 Aug 5.

Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Chungcheongnam, 32439, Republic of Korea. Electronic address:

Owing to the ineffectiveness of the currently used therapies against melanoma, there has been a shift in focus toward alternative therapies involving the use of natural compounds. This study assessed the anticancer effects of oleanolic acid (OA) and its ability to induce apoptosis in A375SM and A375P melanoma cells in vivo. Compared to the control group, viability of A375P and A375SM cells decreased following OA treatment. Read More

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September 2021

Mechanisms of the Cytotoxic Effect of Selenium Nanoparticles in Different Human Cancer Cell Lines.

Int J Mol Sci 2021 Jul 21;22(15). Epub 2021 Jul 21.

Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilove St., 119991 Moscow, Russia.

In recent decades, studies on the functional features of Se nanoparticles (SeNP) have gained great popularity due to their high biocompatibility, stability, and pronounced selectivity. A large number of works prove the anticarcinogenic effect of SeNP. In this work, the molecular mechanisms regulating the cytotoxic effects of SeNP, obtained by laser ablation, were studied by the example of four human cancer cell lines: A-172 (glioblastoma), Caco-2, (colorectal adenocarcinoma), DU-145 (prostate carcinoma), MCF-7 (breast adenocarcinoma). Read More

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Geospatial Cellular Distribution of Cancer-Associated Fibroblasts Significantly Impacts Clinical Outcomes in Metastatic Clear Cell Renal Cell Carcinoma.

Cancers (Basel) 2021 Jul 26;13(15). Epub 2021 Jul 26.

Department of Genitourinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA.

Cancer-associated fibroblasts (CAF) are highly prevalent cells in the tumor microenvironment in clear cell renal cell carcinoma (ccRCC). CAFs exhibit a pro-tumor effect in vitro and have been implicated in tumor cell proliferation, metastasis, and treatment resistance. Our objective is to analyze the geospatial distribution of CAFs with proliferating and apoptotic tumor cells in the ccRCC tumor microenvironment and determine associations with survival and systemic treatment. Read More

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Systematic screening identifies a TEAD4-S100A13 axis modulating cisplatin sensitivity of oral squamous cell carcinoma cells.

J Oral Pathol Med 2021 Aug 6. Epub 2021 Aug 6.

Health Promotion Center, West China hospital, Sichuan University, Chengdu, China.

Background: This study aimed to screen prognosis-related S100 protein family members in human paxpillomaviruses (HPV)-negative oral squamous cell carcinoma (OSCC) and their molecular regulations.

Methods: Bioinformatic screening was conducted based on single-cell RNA-seq data from Puram 2017 dataset and bulk-seq data from the Cancer Genome Atlas (TCGA). HPV-negative OSCC cell lines CAL-27 and SCC-4 were used as in vitro cell models. Read More

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Electroacupuncture stimulation at Yanglingquan acupoint ameliorates hepatic ischemia-reperfusion injury by down-regulating ET-1 to inhibit TAK1-JNK/p38 pathway.

Am J Physiol Gastrointest Liver Physiol 2021 08 4. Epub 2021 Aug 4.

Department of Anesthesiology, Hunan Provincial People's Hospital, Changsha, China.

The current study set out to investigate the molecular mechanism of electroacupuncture (EA) stimulation at Yanglingquan acupoint (GB34) in hepatic ischemia-reperfusion injury (HIRI) in rats via regulation of the endothelin-1 (ET-1) mediated transforming growth factor-β-activated kinase-1 (TAK1)-c-Jun NH2-terminal kinase (JNK)/p38 signaling pathway. First, EA stimulation was applied to the constructed rat model of HIRI at GB34. Subsequently, the activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and myeloperoxidase (MPO) in liver tissues were measured. Read More

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IGF2BP2 knockdown suppresses thyroid cancer progression by reducing the expression of long non-coding RNA HAGLR.

Pathol Res Pract 2021 Sep 13;225:153550. Epub 2021 Jul 13.

Department of Thyroid Surgery, The first Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China. Electronic address:

Background: N6-methyladenosine (m6A), a common internal modification on RNAs, has been found to be closely linked with RNA biosynthesis/metabolism and cancer development. In this text, the roles and molecular mechanisms of m6A-bind protein IGF2BP2 in the development of thyroid cancer (TC) were investigated in vitro.

Methods: IGF2BP2 and lncRNA HAGLR were screened out through multiple public databases such as TCGA, Ualcan, POSTAR2, Starbase, and GEPIA. Read More

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September 2021

Physiological and pathophysiological roles of acidic mammalian chitinase (CHIA) in multiple organs.

Biomed Pharmacother 2021 Jun 9;138:111465. Epub 2021 Apr 9.

Department of Gastroenterology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou Province 563003, China; Digestive Disease Institute of Guizhou Province, Zunyi, Guizhou Province 563003, China; Endoscopy center, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou Province 563003, China. Electronic address:

Acidic mammalian chitinase (CHIA) belongs to the 18-glycosidase family and is expressed in epithelial cells and certain immune cells (such as neutrophils and macrophages) in various organs. Under physiological conditions, as a hydrolase, CHIA can degrade chitin-containing pathogens, participate in Type 2 helper T (Th2)-mediated inflammation, and enhance innate and adaptive immunity to pathogen invasion. Under pathological conditions, such as rhinitis, ocular conjunctivitis, asthma, chronic atrophic gastritis, type 2 diabetes, and pulmonary interstitial fibrosis, CHIA expression is significantly changed. Read More

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Clinical and histological features of fixed drug eruption: a single-centre series of 73 cases with comparison between bullous and non-bullous forms.

Eur J Dermatol 2021 Jun;31(3):372-380

Pathology department, Henri Mondor hospital, AP-HP, 94010 Créteil Cedex, France, Intensive care unit, Henri Mondor hospital, AP-HP, 94010 Créteil Cedex, France, Reference centre for toxic bullous diseases and severe cutaneous adverse reactions TOXIBUL, Créteil, France.

The clinical and pathological aspects of fixed drug eruption (FDE) have been described based on a few case series. To compare bullous FDE (BFDE) and non-bullous FDE (NBFDE) and to determine whether BFDE can be histologically distinguished from other dermatoses presenting with an apoptotic pan-epidermolysis. In this retrospective monocentre study (2005-2016), FDE was classified as BFDE or NBFDE and localized (one anatomical site) or generalized (≥ two sites; GBFDE). Read More

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Cetyltrimethylammonium Bromide Attenuates the Mesenchymal Characteristics of Hypopharyngeal Squamous Cell Carcinoma Through Inhibiting the EGFR/PI3K/AKT Signaling Pathway.

Anticancer Res 2021 Aug;41(8):3789-3799

Department of Medical Research, Tungs' Taichung Metroharbor Hospital, Taichung, Taiwan, R.O.C.

Background/aim: Cetyltrimethylammonium bromide (CTAB), a quaternary ammonium surfactant, was shown to have antitumor effects in a cellular mode of head and neck squamous cell carcinoma (HNSCC), modulating apoptotic and cytotoxic processes. However, the mechanisms by which CTAB exerts its effects against the epithelial- mesenchymal transition in HNSCC remain poorly understood. In the present study, we investigated whether CTAB inhibits cellular mobility and invasiveness of hypopharyngeal squamous cell carcinoma (HPSCC) cells. Read More

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Investigating the Role of the microRNA-34/449 Family in Male Infertility: A Critical Analysis and Review of the Literature.

Front Endocrinol (Lausanne) 2021 1;12:709943. Epub 2021 Jul 1.

Laboratory of Physiology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.

There is a great body of evidence suggesting that in both humans and animal models the microRNA-34/449 (miR-34/449) family plays a crucial role for normal testicular functionality as well as for successful spermatogenesis, regulating spermatozoa maturation and functionality. This review and critical analysis aims to summarize the potential mechanisms which miR-34/449 dysregulation could lead to male infertility. Existing data indicate that miR-34/449 family members regulate ciliogenesis in the efferent ductules epithelium. Read More

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Pyrazoline derivatives as tubulin polymerization inhibitors with one hit for Vascular Endothelial Growth Factor Receptor 2 inhibition.

Bioorg Chem 2021 Sep 1;114:105134. Epub 2021 Jul 1.

School of Chemistry and Chemical Engineering, Nantong University, Nantong, Jiangsu 226019, China. Electronic address:

In this work, to check the effect of the transposition of the rings in typical patterns, a series of pyrazoline derivatives 3a-3t bearing the characteristic 3,4,5-trimethoxy phenyl and thiophene moieties were synthesized and evaluated as tubulin polymerization inhibitors. Basically, as the concise output of our design, a majority of the synthesized compounds showed potency in inhibiting the tubulin polymerization. The top hit, 3q, exhibited potent anti-proliferation activity on cancer cell lines. Read More

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September 2021

Upregulating promotes Hepatocellular Carcinoma progression.

J Cancer 2021 11;12(16):4791-4809. Epub 2021 Jun 11.

Department of Histology and Embryology, Guangxi Medical University.

: Hepatocellular carcinoma (HCC) presents a common malignant tumor worldwide. Although kinectin 1 () is the most frequently identified antigen in HCC tissues, the detailed roles of in HCC remain unknown. This study seeks to clarify the expression status and clinical value of in HCC and to explore the complicated biological functions of and its underlying mechanisms. Read More

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Caspase 9 and Caspase 3 Immunohistochemical Pattern in Skeletal and Cardiac Muscles at Different Times after Death: An Experimental Study on PMI Estimation.

Diagnostics (Basel) 2021 Jun 9;11(6). Epub 2021 Jun 9.

Legal Medicine Section, Department for Health Promotion and Mother-Child Care, University of Palermo, Via del Vespro, 129, 90127 Palermo, Italy.

(1) Background: The estimation of the post mortem interval (PMI) is a challenge for forensic pathologists because data emerging from methods commonly applied are not always conclusive, since several conditions exist that may affect the reliability of these parameters. Thus, new approaches have been proposed to overcome such a limit. In recent years, several studies have been performed on proteins analyzing their expression/degradation patterns in relation to the progressing of the post mortem interval. Read More

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Protein Aggregation Patterns Inform about Breast Cancer Response to Antiestrogens and Reveal the RNA Ligase RTCB as Mediator of Acquired Tamoxifen Resistance.

Cancers (Basel) 2021 Jun 26;13(13). Epub 2021 Jun 26.

iBiMED-Institute of Biomedicine, University of Aveiro, 3810-193 Aveiro, Portugal.

The protein quality control network, including autophagy, the proteasome and the unfolded protein response (UPR), is triggered by stress and is overactive in acquired antiestrogen therapy resistance. We show for the first time that the aggresome load correlates with apoptosis and is increased in antiestrogen-sensitive cells compared to endocrine-resistant variants. LC-MS/MS analysis of the aggregated proteins obtained after 4OH-tamoxifen and Fulvestrant treatment identified proteins with essential function in protein quality control in antiestrogen-sensitive cells, but not in resistant variants. Read More

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CaBir1 functions as an inhibitor-of-apoptosis and affects caspase-like activitiy in Candida albicans.

Fungal Genet Biol 2021 09 28;154:103600. Epub 2021 Jun 28.

Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea. Electronic address:

CaMca1 is the only metacaspase in Candida albicans, which shows structural homology to the mammalian caspases. CaMca1 consists of the caspase domain, the P20 and P10 regions, and the conserved catalytic histidine-cysteine dyad that is required for executing apoptosis in C. albicans. Read More

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September 2021